Composition containing fermented peony seed oil and application of composition in skin care products

Abstract

The invention relates to a composition containing fermented peony seed oil and application of the composition in skin care products, and belongs to the technical field of skin care products, the composition is composed of 20-25 wt% of fermented peony seed oil, 2-4.5 wt% of a saw palmetto fruit extract, 4 wt% of a bitter melon fruit extract, 1.5 wt% of an abelmoschus esculentus fruit extract, 0.5-1 wt% of an emulsifier and the balance of deionized water, the fermented peony seed oil is prepared through a fermentation process, and fermentation strains comprise lactobacillus plantarum and bacillus licheniformis. The composition containing fermented peony seed oil provided by the invention does not contain chemical components with high irritation or high skin burden, is mild and non-irritant, can effectively improve eczema, has a remarkable effect, and is suitable for being used as an active component to be added into a skin care product.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of skin care products, and particularly relates to a composition containing fermented peony seed oil and application thereof in skin care products. BACKGROUND

[0002] Eczema is a typical skin barrier impaired disease, which is caused by congenital barrier weakness or acquired barrier function decline, so that the skin cannot effectively resist external stimuli, and then induces persistent itching, dryness and inflammation. In recent years, due to the acceleration of life rhythm, complex and variable environmental factors and other multiple influences, the incidence of eczema shows an increasing trend year by year, and eczema has become a very common skin problem in modern people. Due to the characteristics of eczema that is easy to recur and difficult to be completely cured, the daily care and non-drug relief methods for eczema are increasingly concerned.

[0003] Under this background, the skin care industry has devoted to the research and development of special soothing and repairing products for eczema, focusing on skin barrier repair, inflammation inhibition and other core improvement paths, and has achieved certain results. However, the application of current related skin care products still has significant technical bottlenecks: on the one hand, the active ingredients of most products have limited effects, and the actual improvement effect is not significant, which cannot play a repair and repair effect in the skin; on the other hand, in order to pursue short-term and visible effects, some skin care products add chemical ingredients with high irritation or high skin burden, which makes it difficult for some people to tolerate, and not only does not bring real improvement, but also further aggravates the discomfort symptoms. SUMMARY

[0004] The purpose of the present application is to overcome the deficiencies of the prior art and provide a composition containing fermented peony seed oil and application thereof in skin care products.

[0005] To achieve the above-mentioned purpose, the technical solution adopted by the present application is as follows:

[0006] Firstly, the present application provides a composition containing fermented peony seed oil, which is composed of fermented peony seed oil, saw palmetto fruit extract, bitter gourd fruit extract, coffee sida fruit extract, emulsifier and deionized water.

[0007] In a preferred embodiment of the present application, the composition is composed of 20-25wt% of fermented peony seed oil, 2-4.5wt% of saw palmetto fruit extract, 4wt% of bitter gourd fruit extract, 1.5wt% of coffee sida fruit extract, 0.5-1wt% of emulsifier and the balance of deionized water.

[0008] In a preferred embodiment of the present application, the composition is prepared by the following steps:

[0009] fermented mulberry seed oil, saw palmetto fruit extract, bitter gourd fruit extract, abelmoschus manihot fruit extract, emulsifier and deionized water are homogenized in a homogenizer; the homogenization refers to stirring at a stirring speed of 2500-4000 rpm for 10-20 min at 40-50 ℃.

[0010] In a preferred embodiment of the present application, the fermented mulberry seed oil is prepared by the following steps:

[0011] The deionized water and the bouillon medium are mixed, sterilized, cooled, inoculated with Lactobacillus plantarum seed liquid and Bacillus licheniformis seed liquid, fermented under air atmosphere, centrifuged, the oil phase is taken, and microfiltration is performed.

[0012] Preferably, the mass ratio of the deionized water, the bouillon medium and the mulberry seed oil is 160-200:5-6.5:25; the inoculation amount of the Lactobacillus plantarum seed liquid is 2-4% of the sum of the mass of the deionized water, the bouillon medium and the mulberry seed oil; the inoculation amount of the Bacillus licheniformis seed liquid is 2-2.5% of the sum of the mass of the deionized water, the bouillon medium and the mulberry seed oil.

[0013] Preferably, the mixing refers to mixing at 25 ℃ for 10-25 min.

[0014] Preferably, the sterilization refers to sterilization at 121 ℃ under a pressure of 1.05 kg / cm 2 .

[0015] Preferably, the cooling refers to cooling to 25-28 ℃ at room temperature.

[0016] Preferably, the cell concentration of the Lactobacillus plantarum seed liquid is 10 7 -10 8 CFU / mL; the Lactobacillus plantarum seed liquid is obtained by culturing Lactobacillus plantarum in MRS medium; the cell concentration of the Bacillus licheniformis seed liquid is 10 7 -10 8 CFU / mL; the Bacillus licheniformis seed liquid is obtained by culturing Bacillus licheniformis in LB medium. Since the culture method of the cells is known in the art, the present application will not be described in detail, and in the technical solution of the present application, only the corresponding cell concentration needs to be ensured.

[0017] Preferably, the fermentation conditions are temperature 30-37 ℃, time 30-40 h, and stirring speed 180-230 rpm.

[0018] Preferably, the centrifugation conditions are speed 10000-12000 rpm and time 15 min.

[0019] Preferably, the microfiltration refers to microfiltration through a polyether sulfone membrane with a pore size of 0.22 µm.

[0020] In a preferred embodiment of the present application, the emulsifier is at least one of Tween 20 and Tween 80.

[0021] Finally, the present application further provides a use of the composition containing fermented peony seed oil in skin care products, wherein the use refers to adding the composition containing fermented peony seed oil into the skin care products, and the adding amount is 20-25% of the mass of the skin care products.

[0022] The present application has the following advantages:

[0023] (1) The present application provides a composition containing fermented peony seed oil, which takes fermented peony seed oil as a basic component. Peony seed oil is rich in α-linolenic acid and other unsaturated fatty acids and sterols. It can alleviate the symptoms of eczema by inhibiting the release of inflammatory factors. At the same time, the rich unsaturated fatty acids can supplement skin lipids and repair the damaged skin barrier, thereby reducing eczema and skin inflammation caused by external stimuli. The fermentation process can further optimize the efficacy. The present application converts large molecular lipids into small molecular lipids that are more easily absorbed through the synergistic fermentation of Lactobacillus plantarum and Bacillus licheniformis, thereby improving the utilization and enhancing the anti-inflammatory and soothing effects.

[0024] (2) The present application takes fermented peony seed oil as a basic component and has achieved certain results in improving eczema. However, there is still room for improvement. Among them, the compounding as a commonly used synergistic technology in the field of skin care has a very big advantage in cost and operation simplicity. Therefore, after long-term research, the inventors found that three kinds of fruit extracts and fermented peony seed oil have a good synergistic effect, which can jointly improve eczema. The three kinds of fruit extracts are saw palmetto fruit extract, bitter gourd fruit extract and coffee yellow lotus fruit extract. Saw palmetto fruit extract contains active ingredients such as saw palmetto fatty acid, which can inhibit the reproduction of bacteria at the eczema site, reduce the stress response and skin sensitivity, and is especially suitable for the early-stage care of eczema, which can strengthen the skin resistance. Bitter gourd fruit extract is rich in bitter gourd saponins and steroidal components, which can form a natural plant barrier on dry and rough eczema skin, repair damaged keratin layer, and gradually restore the density and integrity of the skin at the eczema site. The flavonoids and polysaccharides in coffee yellow lotus fruit extract can affect the trace environment on the skin surface, maintain a more suitable humidity and homeostasis, and delay the outward loss of moisture, providing necessary conditions for barrier repair.

[0025] (3) The composition containing fermented peony seed oil provided by the present application does not add high-stimulating or high-skin-burden chemical components, is mild and non-irritating, can effectively improve the eczema condition, has a remarkable effect, and is suitable for being added as an active ingredient into skin care products. DETAILED DESCRIPTION

[0026] In order to further illustrate the technical means and effects adopted by the present application to achieve the predetermined inventive purpose, the specific embodiments, structures, features and effects according to the present application are described in detail below in combination with examples.

[0027] Some raw materials in the following examples and comparative examples are commercially available, specifically:

[0028] The saw palmetto fruit extract is purchased from Shaanxi Haoyang Biological Technology Co., Ltd., with a specification of saw palmetto fatty acid 25%;

[0029] The bitter gourd fruit extract is purchased from Shaanxi Xinyanghe Biological Technology Co., Ltd., with a specification of 75%;

[0030] The Abrus precatorius fruit extract is purchased from Liupan Yun Biological Technology Co., Ltd., with a specification of 10:1;

[0031] The peony seed oil is purchased from Jiangxi Global Natural Spices Co., Ltd., with a purity of 99%;

[0032] The meat broth medium, MRS medium and LB medium are purchased from Henan Standard Material Research and Development Center, with model numbers of WJ6095, WJ6496 and H17379, respectively;

[0033] The Lactobacillus plantarum and Bacillus licheniformis are purchased from Beina Biological Technology Co., Ltd. (BNCC), with BNCC numbers of BNCC361864 and BNCC168439, respectively.

[0034] The following culture methods of bacterial cells are known in the art, and thus the present application will not be described in detail. In the technical solutions of the present application, only the corresponding bacterial cell concentration needs to be ensured; the Lactobacillus plantarum seed liquid is obtained by culturing Lactobacillus plantarum in MRS medium; and the Bacillus licheniformis seed liquid is obtained by culturing Bacillus licheniformis in LB medium.

[0035] Example 1

[0036] A composition containing fermented peony seed oil, which is composed of 20 wt% of fermented peony seed oil, 2 wt% of saw palmetto fruit extract, 4 wt% of bitter gourd fruit extract, 1.5 wt% of Abrus precatorius fruit extract, 0.5 wt% of Tween 20 and the balance of deionized water.

[0037] The composition is prepared by the following steps:

[0038] The fermented peony seed oil, saw palmetto fruit extract, bitter gourd fruit extract, Abrus precatorius fruit extract, Tween 20 and deionized water are placed in a homogenizer and homogenized at 40°C with a stirring speed of 2500 rpm for 10 min.

[0039] The fermented mulberry seed oil is prepared by the following steps:

[0040] The deionized water and the bouillon medium are mixed under stirring at 25°C for 10 min, sterilized at 121°C for 15 min under a pressure of 1.05 kg / cm 2 , cooled to 25°C at room temperature, the mulberry seed oil is added, inoculated with Lactobacillus plantarum seed liquid (10 7 CFU / mL, inoculation amount is 2% of the sum of the mass of the deionized water, the bouillon medium and the mulberry seed oil) and Bacillus licheniformis seed liquid (10 7 CFU / mL, inoculation amount is 2.1% of the sum of the mass of the deionized water, the bouillon medium and the mulberry seed oil), fermented under an air atmosphere, centrifuged (10000 rpm, 15 min), the oil phase is taken, micro-filtered under a pressure of 0.2 MPa through a polyether sulfone membrane with a pore size of 0.22 µm; the mass ratio of the deionized water, the bouillon medium and the mulberry seed oil is 160:5:25; the fermentation condition is temperature 30°C, time 30 h, stirring speed 180 rpm.

[0041] The application of a composition containing fermented mulberry seed oil in skin care products, the application refers to adding the composition containing fermented mulberry seed oil to the skin care products, the addition amount is 20% of the mass of the skin care products.

[0042] Example 2

[0043] A composition containing fermented mulberry seed oil, the composition is composed of 25wt% fermented mulberry seed oil, 4.5wt% saw palmetto fruit extract, 4wt% bitter gourd fruit extract, 1.5wt% kandahar coffee fruit extract, 1wt% Tween 80 and the balance of deionized water.

[0044] The composition is prepared by the following steps:

[0045] The fermented mulberry seed oil, saw palmetto fruit extract, bitter gourd fruit extract, kandahar coffee fruit extract, Tween 80 and deionized water are placed in a homogenizer, homogenized under stirring at 50°C at a stirring speed of 4000 rpm for 20 min.

[0046] The fermented mulberry seed oil is prepared by the following steps:

[0047] The deionized water and the bouillon medium are mixed under stirring at 25°C for 10 min, sterilized at 121°C for 15 min under a pressure of 1.05 kg / cm 2 , cooled to 25°C at room temperature, the mulberry seed oil is added, inoculated with Lactobacillus plantarum seed liquid (10 8CFU / mL, inoculation amount is 4% of the sum of the mass of the deionized water, the broth medium and the peony seed oil)and bacillus licheniformis seed liquid (10 8 CFU / mL, inoculation amount is 2.5% of the sum of the mass of the deionized water, the broth medium and the peony seed oil), fermentation under air atmosphere, centrifugation (12000 rpm, 15 min), taking the oil phase, microfiltration through a polyether sulfone membrane with a pore size of 0.22 µm under a pressure of 0.2 MPa; the mass ratio of the deionized water, the broth medium and the peony seed oil is 200:6.5:25; the fermentation condition is temperature 37℃, time 40h, stirring speed 230 rpm.

[0048] The application of a composition containing fermented peony seed oil in skin care products, wherein the application refers to adding the composition containing fermented peony seed oil to the skin care products, and the adding amount is 25% of the mass of the skin care products.

[0049] Example 3

[0050] A composition containing fermented peony seed oil, which is composed of 23wt% of fermented peony seed oil, 4wt% of saw palmetto fruit extract, 4wt% of bitter gourd fruit extract, 1.5wt% of kandapahim fruit extract, 0.7wt% of Tween 20 and the balance of deionized water.

[0051] The composition is prepared by the following steps:

[0052] The fermented peony seed oil, saw palmetto fruit extract, bitter gourd fruit extract, kandapahim fruit extract, Tween 20 and deionized water are placed in a homogenizer and homogenized at 45℃ with stirring at a stirring speed of 3000 rpm for 15 min.

[0053] The fermented peony seed oil is prepared by the following steps:

[0054] The deionized water and the broth medium are mixed by stirring at 25℃ for 20 min, sterilized at 121℃ under a pressure of 1.05 kg / cm 2 for 17 min, cooled to 26℃ at room temperature, and then the peony seed oil is added, inoculated with lactobacillus plantarum seed liquid (10 8 CFU / mL, inoculation amount is 4% of the sum of the mass of the deionized water, the broth medium and the peony seed oil)and bacillus licheniformis seed liquid (10 8CFU / mL, inoculation amount was 2.4% of the sum of the mass of the deionized water, the broth medium and the peony seed oil, fermentation under air atmosphere, centrifugation (11000 rpm, 15 min), taking the oil phase, microfiltration through a polyether sulfone membrane with a pore size of 0.22 µm under a pressure of 0.2 MPa; the mass ratio of the deionized water, the broth medium and the peony seed oil was 180:6:25; the fermentation condition was a temperature of 35 ℃, a time of 36 h and a stirring speed of 200 rpm.

[0055] The application of a composition containing fermented peony seed oil in a skin care product, wherein the application refers to adding the composition containing fermented peony seed oil to the skin care product, and the addition amount is 24% of the mass of the skin care product.

[0056] Comparative Example 1

[0057] On the basis of Example 3, equal amounts of bitter gourd fruit extract and coffee sedge fruit extract were used to replace saw palmetto fruit extract at a weight ratio of 4:1.5, and other conditions and settings were the same as those in Example 3.

[0058] Comparative Example 2

[0059] On the basis of Example 3, equal amounts of saw palmetto fruit extract and coffee sedge fruit extract were used to replace bitter gourd fruit extract at a weight ratio of 4:1.5, and other conditions and settings were the same as those in Example 3.

[0060] Comparative Example 3

[0061] On the basis of Example 3, equal amounts of saw palmetto fruit extract and bitter gourd fruit extract were used to replace coffee sedge fruit extract at a weight ratio of 4:4, and other conditions and settings were the same as those in Example 3.

[0062] Comparative Example 4

[0063] On the basis of Example 3, fermented peony seed oil was used to replace saw palmetto fruit extract, bitter gourd fruit extract and coffee sedge fruit extract, respectively, and other conditions and settings were the same as those in Example 3.

[0064] Comparative Example 5

[0065] On the basis of Example 3, Lactobacillus plantarum seed liquid was used to replace Bacillus licheniformis seed liquid, and other conditions and settings were the same as those in Example 3.

[0066] Comparative Example 6

[0067] On the basis of Example 3, Bacillus licheniformis seed liquid was used to replace Lactobacillus plantarum seed liquid, and other conditions and settings were the same as those in Example 3.

[0068] Comparative Example 7

[0069] On the basis of Example 3, use peony seed oil to replace fermented peony seed oil by equal amount, and other conditions and settings are the same as those in Example 3.

[0070] Effect verification experiment:

[0071] 1. Animal experiment

[0072] Specifically comprising the following steps:

[0073] (1) Take 220 healthy guinea pigs, weighing 225-240 g, male;

[0074] (2) Prepare 5wt% and 0.1wt% dinitrochlorobenzene solutions with acetone as the solvent;

[0075] (3) Prepare a skin care product composed of 5wt% glycerol, 0.1wt% hyaluronic acid, 1wt% sweet almond oil, 0.4wt% cetostearyl alcohol polyether-25 and the balance of deionized water;

[0076] (4) Add the compositions prepared in Example 3 and Comparative Examples 1-7 to the skin care product, respectively, in an amount of 24% of the mass of the skin care product, to obtain a total of 9 experimental reagents, including the skin care product without the composition;

[0077] (5) Shave the guinea pigs on the back, size 2cm*2cm, apply 20μL of 5wt% dinitrochlorobenzene solution to the shaved area for sensitization, and after 14 days, apply 100μL of 0.1wt% dinitrochlorobenzene solution to the inner side of the right ear of the guinea pig for challenge, which needs to be challenged 4 times, with 7 days interval between each challenge, and 48h after the last challenge, the guinea pigs are randomly divided into 11 groups, and the guinea pigs are given drugs on the inner side of the right ear respectively;

[0078] (6) The drugs are given in the following manner: 9 groups are respectively given experimental reagents, 1 group is given normal saline, and 1 group is given compound dexamethasone acetate ointment, with a dosage of 25μL each time, once a day, for 14 consecutive days;

[0079] (7) 8h after the last administration, the guinea pigs are cut off both ears, weighed, and the mass difference is calculated to obtain the average value within the group, which represents the degree of swelling, i.e. the improvement effect.

[0080] Table 1. Effect verification of animal experiment

[0081] Mean / mg Normal saline 41.8 Compound dexamethasone acetate ointment 21.9 Skin care product 39.2 Example 3 25.6 Comparative Example 1 27.6 Comparative Example 2 30.8 Comparative Example 3 29.9 Comparative Example 4 32.2 Comparative Example 5 28.1 Comparative Example 6 29.3 Comparative Example 7 31.5

[0082] The results of the animal experiment show that the effect of the present application, i.e. Example 3, is better than that of the comparative examples, and can well improve the eczema condition, with significant effect.

[0083] 2. Volunteer experiment

[0084] Specifically comprising the following steps:

[0085] (1) Select 200 patients with confirmed eczema, half male and half female;

[0086] (2) Randomly divide the patients into 10 groups, with half male and half female in each group;

[0087] (3) Prepare 9 experimental reagents, which are the same as the experimental reagents used in the animal experiment;

[0088] (4) Each group of patients is respectively smeared with experimental reagents and normal saline every day, 3 times a day, continuously for one week, and the patients score the improvement of eczema 24 hours after the last smearing;

[0089] (5) Scoring criteria: 5 points, eczema symptoms completely disappear, 4 points, eczema symptoms mostly disappear, 3 points, eczema symptoms partially disappear, 2 points, eczema symptoms slightly disappear, 1 point, eczema symptoms have no change;

[0090] (6) Score and calculate the average value in the group, at the same time, count the number of patients whose eczema symptoms worsen during the experiment.

[0091] Table 2. Volunteer experiment effect verification

[0092] Mean / min Number of people with increased eczema symptoms Normal saline 1.4 0 Skin care product 1.7 0 Example 3 4.5 0 Comparative Example 1 4.0 0 Comparative Example 2 3.1 0 Comparative Example 3 3.3 0 Comparative Example 4 2.8 0 Comparative Example 5 4.0 0 Comparative Example 6 3.5 0 Comparative Example 7 3.0 0

[0093] The volunteer experiment results show that the effect of the present application, i.e. Example 3, is better than that of the comparative examples, and can improve eczema, at the same time, the ingredients are mild and non-irritating, and have high tolerance.

[0094] The above is only a preferred embodiment of the present application, and does not limit the present application in any form. Although the present application has been disclosed as above with a preferred embodiment, it is not intended to limit the present application. Any person skilled in the art can make some changes or modifications to the above disclosed technical content to obtain equivalent embodiments with equivalent changes, without departing from the technical solution of the present application. Any modification, equivalent change and modification of the above embodiments made in accordance with the technical essence of the present application, without departing from the technical solution of the present application, are still within the scope of the technical solution of the present application.

Claims

1. A composition containing fermented peony seed oil, characterized in that: The composition consists of fermented peony seed oil, saw palmetto fruit extract, bitter melon fruit extract, coffee okra fruit extract, emulsifier, and deionized water.

2. The composition containing fermented peony seed oil according to claim 1, characterized in that: The composition consists of 20-25 wt% fermented peony seed oil, 2-4.5 wt% saw palmetto fruit extract, 4 wt% bitter melon fruit extract, 1.5 wt% okra fruit extract, 0.5-1 wt% emulsifier, and the balance being deionized water.

3. The composition containing fermented peony seed oil according to claim 1, characterized in that: The fermented peony seed oil is prepared through the following steps: Deionized water and broth culture medium were mixed, sterilized, cooled, and peony seed oil was added. The mixture was then inoculated with Lactobacillus plantarum seed liquid and Bacillus licheniformis seed liquid, fermented in air, centrifuged, and the oil phase was collected and microfiltered.

4. The composition containing fermented peony seed oil according to claim 3, characterized in that: The mass ratio of the deionized water, the broth culture medium, and the peony seed oil is 160-200:5-6.5:25; the inoculation amount of the *Lactobacillus plantarum* seed solution is 2-4% of the sum of the mass of the deionized water, the broth culture medium, and the peony seed oil; the inoculation amount of the *Bacillus licheniformis* seed solution is 2-2.5% of the sum of the mass of the deionized water, the broth culture medium, and the peony seed oil.

5. The composition containing fermented peony seed oil according to claim 3, characterized in that: The mixing refers to stirring at 25°C for 10-25 minutes.

6. The composition containing fermented peony seed oil according to claim 3, characterized in that: The bacterial concentration of the *Lactobacillus plantarum* seed solution is 10. 7 -10 8 CFU / mL; the *Lactobacillus plantarum* seed solution was obtained by culturing *Lactobacillus plantarum* on MRS medium; the cell concentration of the *Bacillus licheniformis* seed solution was 10. 7 -10 8 CFU / mL; the Bacillus licheniformis seed solution was obtained by culturing Bacillus licheniformis on LB medium.

7. The composition containing fermented peony seed oil according to claim 3, characterized in that: The fermentation conditions are a temperature of 30-37℃, a time of 30-40 hours, and a stirring speed of 180-230 rpm.

8. The composition containing fermented peony seed oil according to claim 3, characterized in that: The centrifugation conditions were 10,000-12,000 rpm for 15 minutes.

9. The composition containing fermented peony seed oil according to claim 1, characterized in that: The emulsifier is at least one of Tween 20 and Tween 80.

10. The use of a composition containing fermented peony seed oil according to any one of claims 1-9 in a skin care product, characterized in that: The application refers to adding the composition containing fermented peony seed oil to the skin care product, with the addition amount being 20-25% of the mass of the skin care product.