Fly maggot powder and organic selenium grass powder-containing blood fat-reducing pill based on ancient prescription idea

Abstract

A blood fat-reducing pill containing fly maggot powder and organic selenium grass powder based on an ancient prescription idea belongs to the technical field of Chinese patent medicine production, all the raw materials need to be processed into powder, and all the powdery raw materials are crushed into fine powder with the particle size of 200 meshes by a crusher. The feed additive is prepared from the following raw materials in parts by weight: 30-60 parts of fly maggot powder, 20-50 parts of organic selenium grass powder and 10-30 parts of pharmaceutic adjuvants, the pharmaceutic adjuvant comprises one or more of ingredient medicine powder, an adhesive, a filler and a lubricant, the ingredient medicine powder is prepared from hawthorn, semen cassiae, polygonum multiflorum, salvia miltiorrhiza and rhizoma alismatis, and the ingredients are prepared into superfine powder and then are equivalently mixed; experimental results show that the product disclosed by the invention has a remarkable effect of reducing blood fat metabolism. The traditional Chinese medicine composition has the advantages of synergistic interaction, obvious effect, innovative source, ancient use, safety, nature, small side effect, advanced dosage form, convenience in taking, low cost and obvious effect.

Description

Technical Field

[0001] This invention relates to a lipid-lowering pill containing fly larvae powder and organic selenium grass powder, based on ancient medicinal formulas, belonging to the field of traditional Chinese medicine manufacturing technology. Specifically, it relates to a pill composition with the function of assisting in lowering blood lipids, and in particular, a medicinal or health food composition that integrates traditional Chinese medicine theory and uses fly larvae powder and organic selenium grass powder as the main active ingredients. Background Technology

[0002] Hyperlipidemia is a common and prevalent disease in modern society, and a significant risk factor for cardiovascular and cerebrovascular diseases such as atherosclerosis, coronary heart disease, and stroke. Currently, commonly used lipid-lowering drugs such as statins and fibrates are highly effective, but long-term use may lead to side effects such as liver and kidney damage, and muscle pain. Therefore, developing natural, safe, and effective lipid-lowering products is of significant practical importance.

[0003] Traditional Chinese medicine has accumulated rich experience in regulating blood lipids. Many ancient prescriptions (such as the "Zexie Tang" in *Jinkui Yaolue* and modified versions of "Baohe Wan" in *Danxi Xinfa*) embody the approach of treating "phlegm turbidity" and "blood stasis," employing methods such as strengthening the spleen, resolving phlegm, eliminating dampness, and promoting blood circulation to regulate blood lipids. Insect medicines occupy a unique position in the treasure trove of Chinese medicine. While housefly larvae are recorded in classics such as *Compendium of Materia Medica*, they are mostly used for clearing heat and detoxifying, and treating sores; their specific lipid-lowering activity and applications have not been systematically elucidated. Meanwhile, modern nutrition science has discovered that selenium is an essential trace element for the human body and a core component of glutathione peroxidase (GSH-Px). This enzyme can scavenge peroxides and free radicals in the body, protect cell membrane structure, and play an important regulatory role in lipid metabolism. Organic selenium (such as selenomethionine) is highly favored due to its high safety and good bioavailability. Organic selenium grass powder, obtained through biotransformation of grasses (such as selenium-enriched wheatgrass and selenium-enriched wheat seedlings), combines the physiological activity of selenium with phytonutrients.

[0004] While there are reports of using insect protein or selenium products alone for health care in the existing technology, there is no public information on the scientific combination of fly larvae powder, which originates from ancient medicine prescriptions, and organic selenium grass powder to enhance the lipid-lowering effect through synergistic effects, and the preparation of it into convenient pill form. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of existing technologies and provide a lipid-lowering pill that is scientifically formulated, safe, effective, and convenient to take. This pill integrates the ancient Chinese medical concepts of "medicine and food sharing the same origin" and "strengthening the body's resistance and eliminating pathogens," and is manufactured using modern technology. It has a significant effect on lowering serum total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C), while raising high-density lipoprotein cholesterol (HDL-C).

[0006] The technical solution of the present invention is as follows:

[0007] A lipid-lowering pill containing fly larvae powder and organic selenium grass powder, based on ancient medicinal formulas, requires all raw materials to be processed into powder form. All powdered raw materials are pulverized into fine powder with a particle size of 200 mesh using a pulverizer. The pill is characterized by being made from the following raw materials in parts by weight: 30-60 parts fly larvae powder; 20-50 parts organic selenium grass powder; and 10-30 parts pharmaceutical excipients.

[0008] Furthermore, the organic selenium grass powder contains 100-500 μg / kg of organic selenium, and the organic selenium grass powder is prepared by drying and ultra-fine grinding one or more of selenium-enriched wheatgrass, selenium-enriched wheat seedlings or selenium-enriched rice seedlings.

[0009] Furthermore, the pharmaceutical excipients include one or more of the following: pharmaceutical powder, binder, filler, and lubricant. The pharmaceutical powder consists of: hawthorn, cassia seed, fleeceflower root, salvia miltiorrhiza, and alisma plantago-aquatica, each component prepared into ultrafine powder and then mixed in equal amounts; the binder is selected from microcrystalline cellulose, hydroxypropyl methylcellulose (HPMC), and starch paste; the filler is selected from pregelatinized starch, lactose, and mannitol; and the lubricant is selected from magnesium stearate and micronized silica gel.

[0010] Furthermore, the preparation method of this pill includes the following steps:

[0011] 1. Raw material pretreatment: Aseptically cultured housefly larvae are cleaned, freeze-sterilized, and vacuum freeze-dried, then ultra-finely pulverized and passed through a 100-200 mesh sieve to obtain fly larvae powder; selenium-enriched grass is cleaned, dried, ultra-finely pulverized, and passed through a 100-200 mesh sieve to obtain organic selenium grass powder; the ingredient powder is made by purchasing raw materials from the medicinal materials market, then crushing each component into ultra-fine powder, ultra-finely pulverizing, and passing through a 100-200 mesh sieve to obtain ultra-fine powder with a fineness of 100-200 mesh, and then mixing them in equal amounts.

[0012] 2. Mixing: Weigh the fly larvae powder, organic selenium grass powder and pharmaceutical excipients according to the above ratio, put them into the mixer and mix them thoroughly to obtain the mixed powder.

[0013] 3. Pelletizing: Add an appropriate amount of aqueous ethanol solution (such as 70%-80% ethanol) to the mixed powder as a wetting agent and soften the material, and then prepare pellets using a pellet extruder or a pelletizing machine.

[0014] 4. Drying and polishing: Dry the wet pills at a low temperature of 50-60℃ until the moisture content is ≤5%, and then polish them in a polishing pan to obtain the final pill product.

[0015] The beneficial effects of this invention are as follows:

[0016] 1. Synergistic Effect and Significant Efficacy: This invention creatively combines fly larvae powder with organic selenium-enriched grass powder. Fly larvae powder is rich in active substances such as antimicrobial peptides, chitin, and unsaturated fatty acids, which can regulate blood lipids through multiple pathways, including regulating intestinal flora and inhibiting cholesterol absorption. Organic selenium-enriched grass powder provides highly bioavailable selenium, significantly enhancing GSH-Px activity in the body, strengthening antioxidant properties, and protecting vascular endothelial function. The combination of these two ingredients works synergistically, acting on multiple aspects of lipid metabolism, producing a synergistic lipid-lowering effect of "1+1>2".

[0017] 2. Original Innovation, Applying the Past to the Present: This invention draws on the wisdom of "treating diseases with insects" and "dietary therapy" from ancient prescriptions, but does not simply replicate the ancient prescriptions. Instead, it modernizes the traditional medicinal material (fly larvae) (such as ultra-fine grinding to improve bioavailability) and innovatively combines it with "organic selenium" discovered by modern nutrition science, giving the ancient prescriptions new life and a clearer modern scientific connotation.

[0018] 3. Safe and natural with few side effects: All raw materials are derived from nature, avoiding the potential side effects of chemically synthesized drugs. It is highly safe and suitable for sub-healthy people and patients who need long-term lipid regulation.

[0019] 4. Advanced dosage form and convenient administration: Using modern pill preparation technology, the product has stable quality and accurate dosage, and masks some of the unpleasant odors of the raw materials. It is easy to carry and take, and patients have good compliance. Attached Figure Description

[0020] Figure 1 This is a flowchart illustrating the preparation process of a lipid-lowering pill containing fly larvae powder and organic selenium grass powder, based on ancient medicinal formulas.

[0021] Figure 2 This image shows the results of a full biochemical test conducted on a human patient on an empty stomach in the morning before the experiment, before the patient had taken any pills.

[0022] Figure 3 The image shows the results of a full biochemical test conducted on a human on an empty stomach after a 43-day trial of the drug. Detailed Implementation

[0023] The specific embodiments of the present invention will be further described in detail below with reference to the accompanying drawings, examples, and experimental cases. The following examples are for illustrative purposes only and are not intended to limit the scope of the invention.

[0024] Example 1

[0025] I. Preparation of the core ingredients for the pills: fly larvae powder and organic selenium-enriched grass powder

[0026] (I) Technical Scheme for Aseptic Housefly Larvae Rearing

[0027] The core objective of this technology is to produce high-quality fly larvae free from pathogenic microorganisms, parasites, heavy metals, and harmful substance residues under controlled conditions, in order to meet the stringent requirements for pharmaceutical or health food raw materials.

[0028] 1. Core Technology Principles

[0029] By establishing a closed system, the three key elements of housefly (Musca domestica) – breeding stock, feed, and environment – ​​are strictly purified and controlled, blocking the invasion of external pathogens and pollutants, and achieving sterile breeding or reaching specific hygiene standards (such as microbial limit standards).

[0030] 2. Aquaculture facilities and equipment

[0031] 2.1 Fly breeding room: An independent, sealed, positive pressure room equipped with air conditioning, HEPA high-efficiency air filters, ultraviolet lamps and ozone generators to control temperature (25-28℃), humidity (60-70%) and light cycle (12h light / 12h dark).

[0032] 2.2 Larval rearing room: It is also a closed and controllable environment, requiring good ventilation and an exhaust gas treatment system (such as an alkaline spray tower to treat ammonia).

[0033] 2.3 Fly cage: Used for raising adult flies (breeding flies), made of stainless steel or PVC frame and nylon mesh, equipped with sleeve-type operating port, feed tray, drinking tray and egg-laying trough.

[0034] 2.4 Larval rearing boxes / trays: Made of food-grade PP plastic, moderately sized, easy to stack and handle.

[0035] 2.5 Automation System: This includes automatic feeders, temperature and humidity sensors, monitoring cameras, etc., to minimize human intervention.

[0036] 2.6 Sterilization equipment: high-pressure steam sterilizer, ozone generator, ultraviolet lamp, chemical disinfectants (such as 75% ethanol, benzalkonium chloride, etc.).

[0037] 3. Aquaculture process

[0038] 3.1 Aseptic Acquisition and Rearing of Fly Breeders

[0039] 3.1.1 Seed source purification:

[0040] 3.1.1.1 Source: Purchase identified and sterilized housefly strains from authoritative biological research institutions, or collect superior individuals from the wild and then sterilize them.

[0041] 3.1.1.2 Aseptic Treatment: Surface disinfection of fly eggs or pupae. Common method: First, soak in a 1-2% sodium hypochlorite solution for 2-3 minutes, then rinse several times with sterile water, and finally rinse with 75% ethanol for 30 seconds, then air dry on a clean bench. The disinfected eggs or pupae are then transferred to a sterile fly cage for molting.

[0042] 3.1.2 Fly rearing:

[0043] 3.1.2.1 Feed: Feed sterile feed, usually a mixture of high-pressure sterilized milk powder, white sugar and water (ratio of approximately 1:1:some), or special fly food sterilized by cobalt-60 irradiation.

[0044] 3.1.2.2 Drinking water: Provide sterile water or autoclaved milk.

[0045] 3.1.2.3 Egg-laying induction: Place an egg-laying trough in the fly cage, put fermented and autoclaved wheat bran in the trough (humidity controlled at about 60%), and add a small amount of ammonia water (sterilized by a filter membrane) to attract female flies to lay eggs.

[0046] 3.2 Large-scale breeding of aseptic larvae

[0047] 3.2.1 Culture medium preparation:

[0048] 3.2.1.1 Raw materials: mainly wheat bran, soybean meal, corn flour, etc. The formula must meet the nutritional needs of larval growth, and the sources must be stable and free of pesticide residues.

[0049] 3.2.1.2 Sterilization: This is the most crucial step. Mix the prepared culture medium raw materials with an appropriate amount of water (adjust the humidity to 60-65%), put it into a high-temperature resistant container, and sterilize it by high-pressure steam (121℃, more than 30 minutes) or cobalt-60 irradiation to thoroughly kill all microorganisms, insect eggs and weed seeds.

[0050] 3.2.2 Inoculation: In a clean bench or sealed inoculation room, collect sterile fly eggs (which are easier to handle after hatching into newly hatched larvae) and evenly spread them on the surface of the sterilized culture medium using sterile instruments. The inoculation density is generally 1-2 g eggs / kg of culture medium.

[0051] 3.2.3 Larval Rearing and Management:

[0052] 3.2.3.1 Temperature: Maintain at 28-30℃, which is the optimal temperature for larval growth.

[0053] 3.2.3.2 Humidity: The humidity of the culture medium should be maintained at 60-65%, and the ambient air humidity at 70-80%. Excessive humidity can easily lead to the growth of miscellaneous bacteria, while insufficient humidity can affect larval development.

[0054] 3.2.3.3 Ventilation: The breeding box should be covered with a lid with micro-holes (the hole diameter is smaller than the size of the larvae) or placed on a shelf with forced ventilation to ensure that the larvae have sufficient oxygen while expelling CO2 and ammonia.

[0055] 3.2.3.4 Turning the feed: During the middle stage of larval growth (around day 2-3), the feed can be turned once to ensure more thorough contact between the larvae and the feed, more even heat dissipation, and increased yield.

[0056] 3.2.4 Harvesting and Pretreatment:

[0057] 3.2.4.1 Timing: When the larvae have grown to their maximum size (about 4-5 days) and are about to pupate, stop feeding them and let them cleanse themselves for a few hours to empty their intestines.

[0058] 3.2.4.2 Separation: Utilizing the negative phototaxis of larvae, the culture medium and larvae are poured into the separation device together. The larvae will burrow downwards, thus separating from the feed.

[0059] 3.2.4.3 Cleaning and Disinfection: Rinse the harvested larvae with sterile water to remove any residue from their bodies. For final disinfection, briefly soak them in low-concentration ozone water or food-grade hydrogen peroxide water.

[0060] 3.2.4.4 Inactivation: Immediately after cleaning and before processing, inactivation treatment should be carried out to meet hygiene and safety requirements. Common methods include scalding with boiling water (2-3 seconds) or instant steaming. This step can thoroughly kill larvae and inactivate enzymes, prevent spoilage, and fix nutrients.

[0061] 4. Quality Control and Testing

[0062] 4.1 Microbiological Testing: Regularly test seed flies, larvae, feed, and environmental swabs. Indicators include: total bacterial count, coliform bacteria, Salmonella, Staphylococcus aureus, mold and yeast counts, etc. The microbiological limits must meet the standards for pharmaceutical / food raw materials.

[0063] 4.2 Heavy metal and hazardous substance testing: Each batch of finished larvae is tested for heavy metal content such as lead, cadmium, mercury, and arsenic to ensure compliance with the Chinese Pharmacopoeia or national food safety standards.

[0064] 4.3 Nutritional analysis: Regularly test the content of crude protein, crude fat, amino acid composition, chitin, etc., to ensure stable product quality.

[0065] 5. Advantages and Precautions

[0066] 5.1 Advantages:

[0067] 5.1.1 Safe and controllable: It fundamentally eliminates pathogenic microbial contamination, and the product has extremely high safety and consistency.

[0068] 5.1.2 Excellent quality: Larvae grow healthier and have more stable nutritional components in a sterile environment.

[0069] 5.1.3 Environmentally friendly: Closed-loop farming eliminates odor leakage and does not disturb residents.

[0070] 5.2 Precautions:

[0071] 5.2.1 High cost: Equipment investment and operating energy consumption are far higher than those of traditional aquaculture.

[0072] 5.2.2 High technical threshold: It requires interdisciplinary knowledge of microbiology, entomology and automation control.

[0073] 5.2.3 Biosafety: Strict measures must be taken to prevent the escape of breeding flies to avoid ecological problems. All waste gas, wastewater, and waste materials must undergo harmless treatment.

[0074] (II) Production and processing technology scheme for organic selenium grass powder (selenium-enriched wheatgrass powder)

[0075] This solution aims to produce high-quality powder with high organic selenium content, complete nutritional components, and microbiological and heavy metal indicators that meet the requirements for pharmaceutical / health food raw materials.

[0076] 1. Core Objectives

[0077] Selenium-enriched wheat seedlings are produced under controlled conditions through biotransformation (plant transformation) and then processed into powder that meets the following standards:

[0078] 1.1 High organic selenium content: Selenium content is stable at 200-500 μg / kg (or other target range).

[0079] 1.2 High proportion of organic selenium: Organic selenium forms such as selenomethionine account for >85%.

[0080] 1.3 Nutrient retention: Retains chlorophyll, SOD enzyme, vitamins, dietary fiber and other active ingredients to the greatest extent.

[0081] 1.4 Food safety: The limits for microorganisms, heavy metals (lead, cadmium, mercury, arsenic), pesticide residues and other indicators meet the requirements of the Chinese Pharmacopoeia and GB 16740-2014 National Food Safety Standard for Health Food.

[0082] 2. Production process flow

[0083] The entire process can be divided into three main stages: selenium-enriched planting → harvesting and pretreatment → deep processing.

[0084] 2.1 Phase One: Selenium-Enriched Cultivation (Soilless Cultivation - Hydroponics)

[0085] Hydroponics is the preferred method for achieving sterile and standardized production, and it can precisely control the form and dosage of selenium.

[0086] 2.1.1 Variety selection: Select wheat varieties with large biomass, strong selenium enrichment capacity, and high nutritional value (such as "Yu Mai" or specific sprout vegetable varieties).

[0087] 2.1.2 Seed treatment:

[0088] 2.1.2.1 Selection: Remove shriveled seeds, broken seeds, and impurities.

[0089] 2.1.2.2 Disinfection: Soak in a 1-2% sodium hypochlorite solution or a 0.1% potassium permanganate solution for 10-15 minutes to thoroughly kill surface pathogens. Then rinse repeatedly with sterile water until no disinfectant residue remains.

[0090] 2.2.2.3 Soaking and germination: Soak the seeds in warm water (25°C) for 8-12 hours in a sterile environment until they show signs of sprouting.

[0091] 2.1.3 Sowing and Cultivation:

[0092] 2.1.3.1 Cultivation tray: Multi-layer seedling tray made of food-grade PP material.

[0093] 2.1.3.2 Basic nutrient solution formula: Use Hoagland standard nutrient solution or modified formula to provide essential elements such as nitrogen, phosphorus, and potassium.

[0094] 2.1.3.3 Selenium source addition: This is a core technical step.

[0095] 2.1.3.3.1 Selenium source selection: Sodium selenite (Na2SeO3) is preferred. It is easily converted into selenoamino acids in plants, has high biocompatibility, and is cost-effective.

[0096] 2.1.3.3.2 Timing and Concentration of Addition: Add sodium selenite to the nutrient solution when the seedlings reach a height of 3-5 cm. The concentration needs to be precisely controlled, usually 5-15 mg / L (calculated as elemental selenium). Too low a concentration will result in poor enrichment, while too high a concentration will cause selenium toxicity and inhibit growth.

[0097] 2.1.3.3.3 Conversion time: Continue cultivation until the seedlings are 15-20cm tall (about 7-10 days) to ensure that selenium is fully absorbed and converted into organic form.

[0098] 2.1.4 Environmental Control:

[0099] 2.1.4.1 Lighting: LED plant growth lights are used, providing red and blue light spectrum, with a light cycle of 12 hours / day and an intensity of 10,000-15,000 Lux.

[0100] 2.1.4.2 Temperature: Maintain a suitable growth temperature of 18-25℃.

[0101] 2.1.4.3 Humidity: The relative humidity of the air should be controlled at 60-70%.

[0102] 2.1.4.4 Ventilation: Maintain air circulation in the cultivation workshop to reduce mold growth.

[0103] 2.2 Phase Two: Harvesting and Pre-treatment

[0104] 2.2.1 Harvesting:

[0105] Harvest at the end of the vegetative growth period, when the selenium content reaches its peak (approximately 15-20cm in height). Use sterile stainless steel knives or automated harvesting equipment to cut the seedlings 1-2cm from the substrate.

[0106] 2.2.2 Cleaning: Rinse with clean water that meets drinking water standards to remove residual nutrient solution and deposits from the surface. A bubble cleaner or a spray cleaner can be used.

[0107] 2.2.3 Draining: Use a centrifugal dryer to dehydrate, quickly remove excess moisture from the surface, and shorten the subsequent drying time.

[0108] 2.3 Phase Three: Intensive Processing

[0109] 2.3.1 Finishing and Color Protection:

[0110] 2.3.1.1 Objective: To inactivate endogenous enzymes such as peroxidase to prevent enzymatic browning, chlorophyll degradation, and nutrient loss during drying and storage; and to fix the form of organic selenium.

[0111] 2.3.1.2 Method: Use steam blanching or microwave sterilization. Recommended conditions: Steam treatment at 100℃ for 60-90 seconds. Instant high temperature can maximize the preservation of color and heat-sensitive nutrients.

[0112] 2.3.2 Drying:

[0113] 2.3.2.1 Method 1 (Preferred): Vacuum freeze drying

[0114] 2.3.2.1.1 Process: Quick-freeze the blanched wheat seedlings (below -30℃) → place them in a vacuum freeze-drying chamber → allow the ice crystals to sublimate directly under low temperature (-40℃ to -20℃) and high vacuum conditions.

[0115] 2.3.2.1.2 Advantages: The product's form, color, flavor, and nutritional components are almost perfectly preserved; it has good rehydration properties and no loss of organic selenium. It is the optimal drying method for pharmaceutical-grade raw materials.

[0116] 2.3.2.1.3 Disadvantages: High cost.

[0117] 2.3.2.2 Method Two (Secondary Option): Low-Temperature Airflow Drying

[0118] 2.3.2.2.1 Process: After the material is cut into sections, it is fed into a belt dryer or tower dryer and dried rapidly with hot air at 60-75℃.

[0119] 2.3.2.2.2 Advantages: Lower cost, suitable for large-scale production.

[0120] 2.3.2.2.3 Disadvantages: There is some loss of heat-sensitive nutrients.

[0121] 2.3.3 Crushing and Sieving:

[0122] 2.3.3.1 Crushing: Use an ultrafine pulverizer (such as an air jet mill or a cryogenic mill) to crush the dried wheat seedlings.

[0123] 2.3.3.2 Sieving: Pass the powder through a 100-200 mesh sieve to obtain a fine, bright green powder. Ultrafine grinding improves the bioavailability of nutrients and also enhances the taste.

[0124] 2.3.4 Sterilization (optional, but strongly recommended): To ensure that microbiological indicators meet standards, the powder can be sterilized by cobalt-60 irradiation (the dosage is usually 5-10 kGy). This method is carried out at room temperature and has minimal impact on nutrient components and organic selenium forms. Ethylene oxide sterilization can also be used (attention should be paid to residue issues).

[0125] 3. Quality control points and testing indicators

[0126] 3.1 Raw material control: seed purity and germination rate; purity and heavy metal content of nutrient solution and selenium source.

[0127] 3.2 Process control: Selenium concentration, pH value, and EC value of nutrient solution; temperature and humidity of the growth environment; blanching temperature and time; drying temperature.

[0128] 3.3 Finished Product Inspection:

[0129] 3.3.1 Sensory characteristics: color (emerald green), odor (fresh grassy smell), and texture (uniform powder).

[0130] 3.3.2 Physicochemical indicators: total selenium content, organic selenium ratio (to be detected using HPLC-ICP-MS), moisture (≤7%), and ash content.

[0131] 3.3.3 Active ingredients: chlorophyll content, SOD enzyme activity.

[0132] 3.3.4 Safety indicators: total bacterial count, coliform bacteria, mold and yeast; lead, cadmium, total arsenic, total mercury; pesticide residues.

[0133] 4. Technological advantages

[0134] 4.1 Standardization: The entire process is controllable, and the stability between product batches is high.

[0135] 4.2 High safety: Control pollution from the source (hydroponics, sterile environment) and eliminate the risk of heavy metals in the soil.

[0136] 4.3 High efficiency in selenium enrichment: The selenium utilization rate of hydroponics is much higher than that of soil cultivation, and the organic selenium conversion rate is high.

[0137] 4.4 Maximizing Nutrition: Low-temperature processes (freeze-drying, ultra-fine grinding) are used to retain active ingredients to the greatest extent.

[0138] This solution provides a high-standard, industrialized production path that ensures the effectiveness, safety, and quality stability of "selenium-enriched wheatgrass powder" as the core ingredient in lipid-lowering pills.

[0139] II. Pill Preparation

[0140] 1. The pills are made from the following raw materials in parts by weight: 30-60 parts fly larvae powder; 20-50 parts organic selenium grass powder; and 10-30 parts pharmaceutical excipients. In this embodiment, the raw materials are weighed according to the following proportions.

[0141] 1.1 Fly larvae powder: 40kg (made from sterile housefly larvae that have been freeze-dried and then ultra-finely pulverized);

[0142] 1.2 Organic selenium grass powder: 30 kg (selenium-enriched wheatgrass powder with an organic selenium content of 200 μg / kg);

[0143] 1.3 Ingredient powder: 10kg (Composition: hawthorn, cassia seed, he shou wu, dan shen, alisma, raw materials purchased from the medicinal herb market, then each ingredient is crushed into ultrafine powder, ultrafine pulverized, passed through a 100-200 mesh sieve, ultrafine powder fineness 100-200 mesh, then mixed in equal amounts);

[0144] 1.4 Microcrystalline cellulose: 15 kg (filler and binder);

[0145] 1.5 Magnesium stearate: 5 kg (lubricant).

[0146] 2. Preparation method

[0147] 2.1 Raw material mixing: Put all the above raw materials into the mixer and mix for 45 minutes until fully homogeneous.

[0148] 2.2 Adding a wetting agent: Slowly spray an 80% ethanol aqueous solution into the mixed powder as a wetting agent while stirring, to make a suitable soft material that can be formed into a ball when squeezed by hand and easily dispersed when pressed.

[0149] 2.3 Wet pellet rolling: The soft material is put into the pellet extruder to make strips, and then rolled into round pellets in the pellet rolling pan.

[0150] 2.4 Drying wet pellets: Place the wet pellets in a hot air circulating drying oven and dry at 55°C for 4 hours to reduce the moisture content to 4.5%.

[0151] 2.5 Pill polishing: The dried pills are put into a polishing pan for polishing to obtain approximately 1,000,000 pills with smooth surfaces and uniform particle size (each pill weighs approximately 0.1g).

[0152] Experimental Example 1: Lipid-lowering Function of the Pills of the Invention in Rats

[0153] 1. Experimental materials: The pills prepared in Example 1 were used.

[0154] 2. Experimental Animals and Model Establishment: Fifty healthy SD rats were randomly divided into five groups: blank control group, model control group, positive drug group (simvastatin, 5 mg / kg / d), low-dose group of the present invention (0.5 g / kg / d), and high-dose group of the present invention (1.0 g / kg / d). Except for the blank group, the other groups were fed a high-fat diet to induce a hyperlipidemia model.

[0155] 3. Administration method: After successful modeling, each administration group was administered the designed dose by gavage, while the blank group and model group were administered an equal volume of physiological saline by gavage for 4 consecutive weeks.

[0156] 4. Detection indicators: After fasting for 12 hours after the last administration, blood was collected from the orbital cavity, serum was separated, and the contents of TC, TG, LDL-C and HDL-C were detected by a fully automated biochemical analyzer.

[0157] 5. Experimental Results: As shown in Table 1, compared with the model control group, both the high- and low-dose groups of this invention significantly reduced serum TC, TG, and LDL-C levels in hyperlipidemic rats (p<0.05 or p<0.01) and significantly increased HDL-C levels (p<0.05). The results indicate that the product of this invention has a significant lipid-lowering effect.

[0158] Table 1: Effects of the effect on blood lipid levels in hyperlipidemic rats (x±s, n=10)

[0159]

[0160] Note: Compared with the control group, ##P<0.01; compared with the model group, *P<0.05, **P<0.01.

[0161] Experimental Example 2: Human Trial of the Lipid-Lowering Function of the Pills of the Invention

[0162] 1. Experimental materials: The pills prepared in Example 1 were used.

[0163] 2. Food consumption test by the inventor's family: Before the test, family members were asked to go to the hospital to test their blood lipid levels on an empty stomach after getting up in the morning. After that, they took the pills three times a day, morning, noon and evening, with each dose being 1 gram of pills. They took the pills for 43 consecutive days, and then went to the same hospital to test their blood lipid levels again.

[0164] 3. Experimental results: such as Figure 2 and Figure 3 As shown, before the experiment, the blood triglyceride (TG) level was 4.03 mmol / L (reference range 0.45-1.7 mmol / L); the total cholesterol (CHOL) level was 5.33 mmol / L (reference range 2.85-5.7 mmol / L); the high-density lipoprotein (HDL) level was 1.00 mmol / L (reference range 0.93-1.81 mmol / L); and the low-density lipoprotein (LDL) level was 3.60 mmol / L (reference range 2.07-3.63 mmol / L). It can be seen that before the experiment, the inventor's family member had high triglyceride (TG) levels, indicating mild chylous blood. After the experiment, the blood triglyceride (TG) level was measured at 3.22 mmol / L (reference range 0.45-1.7 mmol / L); total cholesterol (CHOL) level was 5.21 mmol / L (reference range 2.85-5.7 mmol / L); high-density lipoprotein (HDL) level was 0.99 mmol / L (reference range 0.93-1.81 mmol / L); and low-density lipoprotein (LDL) level was 3.56 mmol / L (reference range 2.07-3.63 mmol / L). It can be seen that before the experiment, the inventor's family member had high triglyceride (TG) levels, indicating mild chylous blood. After 43 days of use, although the inventor's family member's triglyceride (TG) level remained high, indicating mild chylous blood, the levels of triglyceride (TG), total cholesterol (CHOL), HDL, and LDL all decreased, especially the triglyceride (TG) level, which decreased significantly. The results indicate that the product of this invention has a significant lipid-lowering effect.

[0165] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present invention, and these improvements and modifications assumed above should also be considered within the scope of protection of the present invention.

Claims

1. A lipid-lowering pill containing fly larvae powder and organic selenium-enriched grass powder, based on ancient medicinal formulas, wherein all raw materials are processed into powder, and all powdered raw materials are ultra-finely pulverized into fine powder with a particle size of 200 mesh using an ultra-micro pulverizer, characterized in that... It is made from the following raw materials in parts by weight: 30-60 parts fly larvae powder, 20-50 parts organic selenium grass powder, and 10-30 parts pharmaceutical excipients.

2. The lipid-lowering pill containing fly larvae powder and organic selenium-enriched grass powder, based on ancient medicinal formulas, as described in claim 1, is characterized in that: The organic selenium grass powder contains 100-500 μg / kg of organic selenium, and is prepared by drying and ultra-fine grinding one or more of selenium-enriched wheatgrass, selenium-enriched wheat seedlings or selenium-enriched rice seedlings.

3. A lipid-lowering pill containing fly larvae powder and organic selenium-rich grass powder, based on ancient medicinal formulas, as described in claim 1, is characterized in that: The pharmaceutical excipients include one or more of the following: ingredient powder, binder, filler, and lubricant; the ingredient powder consists of: hawthorn, cassia seed, fleeceflower root, salvia miltiorrhiza, and alisma plantago-aquatica, each component is made into ultrafine powder, and then mixed in equal amounts; the binder is selected from one of microcrystalline cellulose, hydroxypropyl methylcellulose (HPMC), and starch paste; the filler is selected from one of pregelatinized starch, lactose, and mannitol; the lubricant is selected from one of magnesium stearate and micronized silica gel.

4. A lipid-lowering pill containing fly larvae powder and organic selenium-enriched grass powder, based on ancient medicinal formulas, as described in claim 1, is characterized in that... Includes the following steps: (a) Raw material pretreatment: Aseptically cultured housefly larvae are cleaned, freeze-sterilized, and vacuum freeze-dried, then ultra-finely pulverized and passed through a 100-200 mesh sieve to obtain fly larvae powder; selenium-enriched grass is cleaned, dried, ultra-finely pulverized, and passed through a 100-200 mesh sieve to obtain organic selenium grass powder; the ingredients are made by purchasing raw materials from the medicinal materials market, then crushing each component into ultra-fine powder, ultra-finely pulverizing, and passing through a 100-200 mesh sieve to obtain ultra-fine powder with a fineness of 100-200 mesh, and then mixing them in equal amounts; (b) Fly larvae powder, organic selenium grass powder, and pharmaceutical excipients are weighed according to the formula, put into a mixer and mixed thoroughly to obtain a mixed powder; (c) A wetting agent is added to the mixed powder to make a soft material, then pelleted, dried, and polished to obtain the final product.

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