Sisal hemp residue vegetable seedling organic fertilizer and preparation method and application thereof

Abstract

The invention provides a sisal hemp residue vegetable seedling organic fertilizer and a preparation method and application thereof. The sisal hemp residue vegetable seedling organic fertilizer comprises a first fertilizer bag, the first fertilizer bag is prepared by fermenting the following raw materials in parts by weight through a microbial composition: 90-150 parts of fresh sisal residues, 5-20 parts of sugarcane leaves, 5-20 parts of an organic nitrogen source, 2-5 parts of molasses, 0.5-1 part of monopotassium phosphate, 0.05-0.2 part of magnesium sulfate and 0.3-0.8 part of calcium superphosphate; the sisal hemp residues and other raw materials are compounded for vegetable seedling raising, nutrients are rich, the vegetable planting benefits are improved, and the planting cost is reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of organic fertilizer processing, and particularly relates to a sisal residue vegetable seedling raising organic fertilizer and a preparation method and application thereof. BACKGROUND

[0002] Sisal is a perennial monocotyledonous fiber crop with tropical characteristics. Its leaf fiber is the most widely used hard fiber in the world today, and is widely used in important fields such as national defense, fisheries, navigation, petroleum and mining. Sisal is native to Mexico and mainly distributed in Africa, Latin America and Asia.

[0003] Sisal residue is a by-product after sisal fresh leaves are processed, accounting for 95% to 97% of sisal leaves. The sisal residue produced each year amounts to millions of tons. Research shows that the sisal residue is not only abundant in yield, but also rich in organic matter and nutrients necessary for plant growth, and is a high-quality organic fertilizer. However, the sisal residue is not effectively utilized, and most of the sisal residue and juice is discarded or discharged around the fiber processing plant, causing great resource waste and environmental pollution. Resource utilization of agricultural and forestry waste is an effective way to utilize organic waste. In-situ material and development of environmentally friendly agricultural organic fertilizer are increasingly valued. At present, some people use sugarcane residue, Chinese medicine residue and mushroom residue as crop cultivation organic fertilizer and achieve good results. However, the fertilizer utilization of sisal residue in China is still at the stage of direct field covering, and most of the sisal residue is directly applied to sisal fields. However, the traditional sisal residue field covering method has the disadvantages of low utilization rate, long fermentation period and low composting efficiency. Therefore, further development of sisal residue into organic fertilizer with better fertilizer efficiency has become an important research direction for extending the sisal industry chain. SUMMARY

[0004] The present application provides a sisal residue vegetable seedling raising organic fertilizer and a preparation method and application thereof. The sisal residue is compounded with other raw materials for vegetable seedling raising, and is rich in nutrients, which is beneficial to improve the vegetable planting benefit and reduce the planting cost.

[0005] To achieve the above-mentioned purpose, the technical scheme of the present application is as follows:

[0006] In one aspect, the present application provides a sisal residue vegetable seedling raising organic fertilizer, which comprises a first fertilizer package, and the first fertilizer package is prepared by microbial composition fermentation of the following material raw materials in parts by weight: fresh sisal residue 90-150 parts, sugarcane leaves 5-20 parts, organic nitrogen source 5-20 parts, molasses 2-5 parts, potassium dihydrogen phosphate 0.5-1 part, magnesium sulfate 0.05-0.2 part, and calcium superphosphate 0.3-0.8 part.

[0007] The microbial composition is composed of Trichoderma asperellum, Bacillus subtilis, Bacillus megaterium, actinomycetes in a weight ratio of (5-10):(2-8):(1-3):(1-2).

[0008] Preferably, a second fertilizer package is further included, which is composed of sisal extract and garlic extract in a weight ratio of 1-5:10-15, and the weight ratio of the second fertilizer package to the first fertilizer package is 1:90-500.

[0009] Preferably, the organic nitrogen source is peanut cake powder, soybean cake powder or corn syrup.

[0010] Preferably, the preparation method of the sisal extract comprises the following steps: taking sisal leaves, chopping, drying, crushing, adding 10-20 times weight of methanol, extracting at room temperature for 36-48 hours, separating sisal extract, repeating the above methanol extraction step 1-3 times, combining the sisal extract, and reducing pressure to concentrate to obtain sisal methanol extract.

[0011] Preferably, the preparation method of the garlic extract comprises the following steps: slicing garlic bulbs, adding alliinase, mixing and sealing for enzyme hydrolysis for 2-6 hours, then adding 5-10 times weight of 90-95% ethanol solution, soaking at room temperature, ultrasonic extraction for 36-48 hours, separating the garlic extract, repeating the above ethanol extraction step 1-3 times, combining the garlic extract, and reducing pressure to concentrate to obtain the garlic extract.

[0012] The second aspect of the application provides a preparation method of the sisal residue vegetable seedling organic fertilizer described above, and the preparation method of the first fertilizer package comprises the following steps:

[0013] (1) taking Trichoderma asperellum, Bacillus subtilis, Bacillus megaterium and actinomycetes, respectively activating them in PDA medium, LB medium, LB medium and Gause No. 1 medium to a spore content of not less than 1×10 8 CFU / g, mixing them in a weight ratio to obtain the microbial composition;

[0014] (2) chopping fresh sisal residue and sugarcane leaves to a length of not more than 3 cm, weighing fresh sisal residue, sugarcane leaves, organic nitrogen source, molasses, potassium dihydrogen phosphate, magnesium sulfate and superphosphate according to weight parts to obtain mixed materials, adjusting the moisture content of the mixed materials to 40-50%, inoculating the microbial composition according to an inoculation amount of 0.1-0.5%, and mixing and fermenting for 20-28 days, and then drying at a temperature of not higher than 40℃, crushing to obtain the first fertilizer package.

[0015] The third aspect of the present application provides the application of the above-mentioned organic fertilizer for vegetable seedling raising prepared from sisal hemp dregs in the cultivation of vegetable seedling raising.

[0016] The fourth aspect of the present application provides the application of the above-mentioned organic fertilizer for vegetable seedling raising prepared from sisal hemp dregs in the prevention and treatment of soft rot in the cultivation of vegetable seedling raising.

[0017] The above-mentioned organic fertilizer for vegetable seedling raising is prepared from fresh sisal hemp dregs as the main fermentation material, and sugarcane leaves and organic nitrogen source as auxiliary materials, and is formed into high-nutrient organic fertilizer after composting, which can provide sufficient nutrients for vegetable seedling raising, improve the germination rate of vegetables and promote root growth, and can also be used in the whole process of vegetable cultivation to improve the yield of vegetables.

[0018] Further, the present application also introduces natural extracts from sisal hemp and garlic, which have good prevention and treatment effect on soft rot of vegetables, and when used in combination with the organic fertilizer prepared by fermentation of sisal hemp dregs, can better improve the soil environment, promote nutrient absorption and inhibit the growth of soil-borne pathogenic bacteria.

[0019] The present application uses sisal hemp dregs to prepare organic fertilizer, which can effectively improve the fertilizer utilization rate of sisal hemp dregs, provides a broader idea for efficient recycling of resources and promotes the development of green ecology in agriculture, and also provides important technical support for extending the sisal hemp industry chain. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical scheme and advantages of the present application clearer, the technical scheme of the present application will be described clearly and completely below in combination with the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0021] Example 1: Screening of the first fertilizer package

[0022] 1.1 Preparation of organic fertilizer

[0023] Pre-treatment before experiment: After removing impurities such as random fibers from fresh sisal hemp dregs, the sisal hemp dregs were used for the experiment in this embodiment.

[0024] Example 1-1

[0025] An organic fertilizer for vegetable seedling raising prepared from sisal hemp dregs, which is prepared from the following material raw materials by microbial composition fermentation: fresh sisal hemp dregs 120 parts, sugarcane leaves 10 parts, corn syrup 20 parts, sugar cane 2 parts, potassium dihydrogen phosphate 0.8 parts, magnesium sulfate 0.1 parts, superphosphate 0.5 parts;

[0026] The microbial composition is composed of Trichoderma asperellum, Bacillus subtilis, Bacillus megaterium and Actinomyces according to a weight ratio of 8:6:2:1.5.

[0027] The preparation method comprises the following steps:

[0028] (1) Take Trichoderma asperellum, Bacillus subtilis, Bacillus megaterium and Actinomyces, respectively activate them in PDA medium, LB medium, LB medium and Gao's No. 1 medium to a spore content of not less than 1×10 8 CFU / g, and mix them according to a weight ratio to obtain the microbial composition;

[0029] (2) Fresh sisal residue and sugarcane leaves are cut to a length of not more than 3 cm, and then fresh sisal residue, sugarcane leaves, organic nitrogen source, molasses, potassium dihydrogen phosphate, magnesium sulfate and superphosphate are weighed according to weight parts to obtain mixed materials, the moisture content of the mixed materials is adjusted to 50%, the microbial composition is inoculated according to an inoculation amount of 0.3%, and then mixed fermentation is carried out for 21 days; after fermentation is completed, drying is carried out at a temperature of not more than 40℃, and then crushing is carried out to obtain the sisal residue vegetable seedling organic fertilizer.

[0030] Comparative Example 1-1

[0031] A sisal residue vegetable seedling organic fertilizer is prepared by fermenting fresh sisal residue by a microbial composition. The microbial composition is composed of Trichoderma asperellum, Bacillus subtilis, Bacillus megaterium and Actinomyces according to a weight ratio of 8:6:2:1.5.

[0032] The preparation method is referred to Example 1-1, and the mixed materials are replaced by fresh sisal residue.

[0033] Comparative Example 1-2

[0034] The microbial composition is composed of Bacillus subtilis, Bacillus megaterium and Actinomyces according to a weight ratio of 6:2:1.5.

[0035] Comparative Example 1-3

[0036] A sisal residue vegetable seedling organic fertilizer is prepared by spreading fresh sisal residue on the ground and naturally fermenting for 21 days, and then drying at a temperature of not more than 40℃ and crushing to obtain the sisal residue vegetable seedling organic fertilizer.

[0037] Comparative Example 1-4

[0038] A sisal residue vegetable seedling organic fertilizer is prepared by taking fresh sisal residue, drying at a temperature of not more than 40℃ and crushing to obtain the sisal residue vegetable seedling organic fertilizer.

[0039] 1.2 Test of various indexes of the organic fertilizer

[0040] 1.2.1 Analysis of the components of the organic fertilizer of sisal residue

[0041] The nutritional components of Example 1-1 and Comparative Examples 1-1 to 1-4, including pH, organic matter, total nitrogen, P2O5, and K2O, were analyzed, and the results are shown in Table 1.

[0042] Table 1 Analysis of the components of the organic fertilizer of sisal residue

[0043]

[0044] As can be seen from Table 1, the fresh sisal residue of Comparative Example 1-1, which was simply fermented by adding the microbial composition, had a lower pH value than the fresh sisal residue of Comparative Example 1-4 and the naturally fermented sisal residue of Comparative Example 1-3. The organic fertilizer of sisal residue prepared by microbial fermentation had a neutral pH value, and the contents of organic matter, total nitrogen, P2O5, and K2O were all improved. Among them, the content of organic matter was increased by 3.38% and 60.26% compared with the fresh sisal residue and the naturally fermented sisal residue, respectively; the total nitrogen was increased by 35.72% and 7.54%, respectively; P2O5 was increased by 92.86% and 35%, respectively; and K2O was increased by 67.05% and 51.13%, respectively.

[0045] Compared with the simple microbial fermentation of fresh sisal residue, the addition of sugarcane leaves, organic nitrogen sources, and inorganic salts further improved the content of organic matter in the organic fertilizer. The content of organic matter in Example 1-1 was increased by 14.58% compared with Comparative Example 1-1, indicating that the added components were beneficial to further improve the activity of microorganisms in the microbial composition, thereby improving the fertilizer utilization rate of sisal residue and sugarcane leaves. In addition, other nitrogen, phosphorus, potassium, and other components were also improved to a certain extent, making them provide more comprehensive nutrients for crop growth.

[0046] The heavy metal content in the above-mentioned organic fertilizer meets the requirements of the organic fertilizer standard, and the technical application can effectively reduce the environmental risk of heavy metal pollution.

[0047] 1.2.2 Effect of sisal residue organic fertilizer on seed germination of vegetables

[0048] Referring to the organic fertilizer treated in Example 1-1 and Comparative Examples 1-1 to 1-3, 130g was sampled every 7 days of fermentation, 3 replicates were taken each time for each treatment, and a total of 4 times (7d, 14d, 21d, 28d) were sampled. Tap water 500ml was added to each treatment, soaked for 24h, and then filtered with double-layer gauze to obtain sisal residue fermentation liquor. The seed germination index was used as an indicator of compost maturity. 2mL of sisal residue fermentation liquor of different treatments was taken in a culture dish lined with filter paper, 30 seeds of fully-grown Chinese cabbage were placed in each culture dish, and the culture dishes were placed at room temperature for 48h. The number of seedlings and root length were measured, and the seed germination index (GI) was calculated. Each sample was repeated 3 times, and water was used as a control.

[0049] The seed germination index (GI) is calculated as follows:

[0050]

[0051] The results are shown in Table 2-4:

[0052] Table 2. Changes in seed germination rate of sisal residue organic fertilizer under different treatments.

[0053]

[0054] Table 3. Changes in root length of seeds treated with sisal residue organic fertilizer

[0055]

[0056] Table 4. Changes in seed germination index (GI value) of sisal residue organic fertilizer under different treatments

[0057]

[0058] As shown in Tables 2-4, the addition of microbial fermentation to sisal residue organic fertilizer can promote the root growth of cabbage seeds. Furthermore, compared with simple sisal residue fermentation, the organic fertilizer with added sugarcane leaves, organic nitrogen sources, and inorganic salts can better improve the germination rate of cabbage and promote the root growth of cabbage seeds. In addition, as a biological indicator, the GI value can reflect the phytotoxicity of fermented compost and is considered the most accurate and effective indicator for evaluating compost maturity. Generally, a GI value greater than 50% can be considered that the compost is basically non-toxic to seeds and that the compost is basically mature; a GI value greater than 80% can be considered that the compost is fully mature. As shown in Table 4, the GI values ​​of the fertilizers in Examples 1-1, Comparative Examples 1-1, and Comparative Examples 1-2 were all greater than 80% on day 7 after the addition of microbial fermentation, while the GI value of the organic fertilizer with added sugarcane leaves, organic nitrogen sources, and inorganic salts reached its highest value after 21 days.

[0059] Example 2: Screening of the second fertilizer pack (soft rot fungicide composition)

[0060] 2.1 Materials

[0061] Test strain: Soft rot pathogen, collected from Chinese cabbage infected with soft rot, isolated and purified by the strain.

[0062] Test reagents: sisal extract and garlic extract.

[0063] 2.2 Preparation method of sisal extract

[0064] (1) Take the healthy plant leaves of sisal (variety: Guangxi 76416), clean them, cut into pieces of about 3 cm x 3 cm, place them in a constant temperature drying oven, control the temperature at 105°C, and kill green for 1 hour, then adjust the temperature to 75°C, dry to constant weight, crush, obtain sisal leaf dry powder, add 10 times the weight of methanol, soak at room temperature for 48 hours, separate the extraction liquid, repeat the above methanol extraction steps 3 times, combine the extraction liquids, and reduce pressure to concentrate to obtain the sisal extract.

[0065] 2.3 Preparation method of garlic extract

[0066] Cut the garlic cloves into slices with a thickness of not more than 0.8 cm, add allinase, mix, seal and enzymatically hydrolyze for 2-6 hours, then add 10 times the weight of 95% ethanol solution, soak at room temperature, ultrasonic extraction for 48 hours, separate the garlic extraction liquid, repeat the above ethanol extraction steps 2 times, combine the garlic extraction liquids, reduce pressure to recover ethanol, and obtain the garlic extract.

[0067] 2.4 Antibacterial test of sisal extract and garlic extract

[0068] Dissolve the sisal extract and garlic extract in ethanol in advance, then dilute them into 5 mass concentrations of each single agent mother liquor and different ratios of mixed agents using 0.1% Tween-80 aqueous solution.

[0069] Method for preparing drug-containing medium: under sterile conditions, according to the test treatment, add the pre-melted sterilized PDA medium to a sterile conical flask, quantitatively draw each single agent mother liquor and mixed agent from low concentration to high concentration in turn, and then add them to the conical flask, then pour an equal amount into three culture dishes with a diameter of 9 cm to prepare the corresponding concentration of drug-containing medium, and set up a blank treatment without drug agents.

[0070] The mycelial growth rate method is used to determine the inhibition rate: take the pathogenic bacteria pre-cultured on the PDA medium, use a 5 mm diameter puncher to cut a consistent mycelial cake at the edge of the colony, transplant it to the center of the culture dish containing the drug-containing medium or the blank control, repeat three times for each treatment, and cultivate at 26°C upside down. When the colony grows to 2 / 3 of the diameter of the culture dish, measure and record the colony diameter using the cross method, and calculate the inhibition rate using the following formula:

[0071]

[0072] Based on the above inhibition rate calculation formula, use SPSS statistical software to obtain the virulence regression equation to further calculate the EC 50 of each agent on the soft rot pathogen.

[0073] According to the method of Sun Yunpei, calculate the co-toxicity coefficient (CTC) to evaluate the synergistic effect of the agents.

[0074] Actual toxicity index (ATI) = (standard EC 50 ÷ mixture EC 50 ) x 100;

[0075] Theoretical toxicity index (TTI) = A agent toxicity index x percentage of A in mixture + B agent toxicity index x percentage of B in mixture;

[0076] Co-toxicity coefficient (CTC) = [mixture actual toxicity index (ATI) ÷ mixture theoretical toxicity index (TTI)] x 100.

[0077] According to the joint action classification standard: co-toxicity coefficient (CTC) ≥ 120 shows synergistic effect; co-toxicity coefficient (CTC) ≤ 80 shows antagonistic effect; 80 < co-toxicity (CTC) < 120 shows additive effect.

[0078] The test results are shown in Table 5:

[0079] Table 5 Toxicity test results of soft rot pathogen by sisal extract and garlic extract

[0080]

[0081] As can be seen from Table 5, after compounding the garlic extract and the sisal extract, the co-toxicity coefficients of the soft rot pathogen in the range of 1-5:10-15 by weight are all greater than 120, showing synergistic effect.

[0082] Example 3 First fertilizer package and second fertilizer package mixing experiment

[0083] The organic fertilizer (first fertilizer package) of Example 1-1, Comparative Example 1-1 and Comparative Example 1-2 was mixed with the second fertilizer package (soft rot bactericidal composition: garlic extract 1:sisal extract 10) to perform the test.

[0084] Ten treatments were set for the test, each with three repetitions, and the details are as follows:

[0085] CK: no organic fertilizer + no conventional fertilization;

[0086] Treatment 1: no organic fertilizer + conventional fertilization;

[0087] Treatment 2: Example 1-1 organic fertilizer + conventional fertilization;

[0088] Treatment 3: Comparative Example 1-1 organic fertilizer + conventional fertilization;

[0089] Treatment 4: Comparative Example 1-2 organic fertilizer + conventional fertilization;

[0090] Treatment 5: Example 1-1 organic fertilizer + second fertilizer package + conventional fertilization;

[0091] Treatment 6: Comparative Example 1-1 organic fertilizer + second fertilizer package + conventional fertilization;

[0092] Treatment 7: Comparative Example 1-2 organic fertilizer + second fertilizer package + conventional fertilization;

[0093] Treatment 8: second fertilizer package + conventional fertilization;

[0094] Treatment 9: Example 1-1 organic fertilizer + 72% agricultural streptomycin seed soaking + conventional fertilization.

[0095] The amount of conventional fertilization for each treatment is: urea 1 g·kg -1 , calcium magnesium phosphate 2 g·kg -1 , potassium chloride 1 g·kg -1 , the application amount of organic fertilizer is 375 g·kg -1 , and the amount of second fertilizer package is 4 g·kg -1 . The calcium magnesium phosphate, potassium chloride and organic fertilizer are mixed with the soil as base fertilizer before transplanting, and 1 / 2 of the urea is base fertilizer and 1 / 2 is topdressing (after 15 days of planting); for the treatment group using organic fertilizer and fertilizer package, the second fertilizer package is mixed with the soil after 5 days of base fertilizer and soil mixing and before planting; for the treatment group using the second fertilizer package alone, the second fertilizer package is mixed with the soil before planting; 72% agricultural streptomycin seed soaking, the specific steps are to dilute the agricultural streptomycin 2000 times and soak the seeds for 1 h. The cultivation pots are ordinary plastic pots with specifications of: mouth diameter 23 cm, bottom diameter 18 cm, height 21 cm, and filled with 2 kg of air-dried soil (the air-dried soil is taken from the soil in which the gold vegetable crisp sweet four seasons pak choi was planted, and the pak choi planted in the soil was infected with soft rot). The test crop is gold vegetable crisp sweet four seasons pak choi, and 20 seeds are sown per pot.

[0096] After the above treatments, the incidence of pak choi soft rot was investigated at the harvest period, and the control effect was calculated.

[0097] Pak choi soft rot investigation grading standard:

[0098] 0 level: no lesion;

[0099] 1 level: lesion area accounts for less than 5% of the whole area;

[0100] 2 level: lesion area accounts for 6-10% of the whole area;

[0101] 3 level: lesion area accounts for 11-20% of the whole area;

[0102] 4 level: lesion area accounts for 21-50% of the whole area;

[0103] 5 level: lesion area accounts for more than 50% of the whole area.

[0104] The following two formulas are used to calculate the prevention and control effect:

[0105] Disease index = [(∑ (number of disease-grade plants × representative grade)) / (total number of plants × highest representative grade value)] × 100

[0106] Prevention and control effect (%) = [(disease index of control group - disease index of treatment group) / disease index of control group] × 100%.

[0107] Meanwhile, when the bok choy matures, it is collected, rinsed clean with water, dried to constant weight, weighed, and its biomass is calculated, with the average value taken. After the bok choy is removed, approximately 400g of soil sample is taken from the root system from top to bottom, roots and other impurities are removed, and the sample is air-dried in a ventilated place before its various physicochemical indicators are tested.

[0108] The effectiveness of soft rot disease control is shown in Table 6:

[0109] Table 6 Results of the efficacy test for soft rot disease control

[0110]

[0111] As can be seen from the results in Table 6, the use of organic fertilizer from the first fertilizer pack in combination with that from the second fertilizer pack can effectively prevent the occurrence and spread of soft rot in the soil.

[0112] The results of the survey on the biomass of Chinese cabbage are shown in Table 7:

[0113] Table 7 Results of the survey on the biomass of Chinese cabbage

[0114]

[0115] As can be seen from the results in Table 7, the organic fertilizer with the first fertilizer pack added significantly increased the biomass of Chinese cabbage compared to treatment 1.

[0116] The results of soil physicochemical index tests are shown in Table 8:

[0117] Table 8. Effects of organic fertilizer on soil physicochemical properties

[0118]

[0119] As can be seen from the results in Table 8, the organic fertilizer of Example 1-1 significantly increased the content of organic matter, total nitrogen, alkaline nitrogen, available phosphorus, and available potassium in the soil compared to treatment 1. After adding the second fertilizer pack, the content of organic matter, total nitrogen, alkaline nitrogen, available phosphorus, and available potassium remained at a high level. Combined with the control of soft rot by the second fertilizer pack, it can better promote the healthy growth of Chinese cabbage, and the biomass is higher than that of other treatments.

[0120] The above merely illustrates the specific embodiments of the present application, but the protection scope of the present application is not limited thereto, any person skilled in the art can easily think of the changes or replacements within the technical range disclosed by the present application, which should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. An organic fertilizer for vegetable seedling cultivation using sisal residue, characterized in that: The fertilizer includes a first fertilizer pack, which is made by fermentation of the following raw materials in parts by weight using a microbial composition: 90-150 parts fresh sisal residue, 5-20 parts sugarcane leaves, 5-20 parts organic nitrogen source, 2-5 parts molasses, 0.5-1 part potassium dihydrogen phosphate, 0.05-0.2 parts magnesium sulfate, and 0.3-0.8 parts superphosphate. The microbial composition consists of Trichoderma hygroscopicum, Bacillus subtilis, Bacillus megaterium, and actinomycetes in a weight ratio of (5-10):(2-8):(1-3):(1-2).

2. The sisal residue organic fertilizer for vegetable seedling cultivation according to claim 1, characterized in that: It also includes a second fertilizer pack, which consists of sisal extract and garlic extract in a weight ratio of 1-5:10-15, and the weight ratio of the second fertilizer pack to the first fertilizer pack is 1:90-500.

3. The sisal residue organic fertilizer for vegetable seedling cultivation according to claim 1, characterized in that: The organic nitrogen source is peanut cake powder, soybean cake powder, or corn syrup.

4. The organic fertilizer for vegetable seedling cultivation based on sisal residue according to claim 2, characterized in that: The preparation method of the sisal extract includes the following steps: take sisal leaves, chop, dry, pulverize, add 10-20 times the weight of methanol, extract at room temperature for 36-48 hours, separate the sisal extract, repeat the above methanol extraction step 1-3 times, combine the sisal extracts, and concentrate under reduced pressure to obtain the sisal extract.

5. The sisal residue organic fertilizer for vegetable seedling cultivation according to claim 2, characterized in that: The preparation method of the garlic extract includes the following steps: garlic bulbs are sliced, alliinase is added, the mixture is sealed and enzymatically hydrolyzed for 2-6 hours, then 5-10 times the weight volume of 90-95% ethanol solution is added, the mixture is soaked at room temperature, ultrasonically extracted for 36-48 hours, the garlic extract is separated, the above ethanol extraction steps are repeated 1-3 times, the garlic extracts are combined, and concentrated under reduced pressure to obtain the garlic extract.

6. The method for preparing organic fertilizer for vegetable seedling cultivation using sisal residue according to any one of claims 1-5, characterized in that: The preparation method of the first fertilizer pack includes the following steps: (1) Take Trichoderma echinosporum, Bacillus subtilis, Bacillus megaterium, and actinomycetes, and activate them respectively with PDA medium, LB medium, LB medium, and Gao's No. 1 medium until the spore content is not less than 1×10⁻⁶. 8 The microbial composition is obtained by mixing CFU / g at a weight ratio; (2) Fresh sisal residue and sugarcane leaves are chopped to a length not exceeding 3cm. Fresh sisal residue, sugarcane leaves, organic nitrogen source, molasses, potassium dihydrogen phosphate, magnesium sulfate and superphosphate are weighed according to the weight to obtain a mixture. The moisture content of the mixture is adjusted to 40-50%. The microbial composition is then inoculated at an inoculation rate of 0.1-0.5%. The mixture is fermented for 20-28 days. After fermentation, it is dried at a temperature not exceeding 40℃ and pulverized to obtain the first fertilizer pack.

7. The application of the sisal residue vegetable seedling organic fertilizer according to any one of claims 1-5 or the sisal residue vegetable seedling organic fertilizer prepared by the preparation method according to claim 6 in vegetable seedling cultivation.

8. The application of the sisal residue vegetable seedling organic fertilizer according to any one of claims 1-5 or the sisal residue vegetable seedling organic fertilizer prepared by the preparation method according to claim 6 in the prevention and control of soft rot in vegetable seedling cultivation.

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