Composition for repairing sensitive skin tough skin barrier and preparation method and application thereof

Abstract

The invention discloses a composition for repairing a tough skin barrier of sensitive skin and a preparation method and application thereof, the composition for repairing the tough skin barrier of the sensitive skin comprises bisabolol, a ginger root extract and ceramide, and the mass ratio of the bisabolol to the ginger root extract to the ceramide is (0.001-5): (0.0001-1): (0.001-5). The bisabolol has anti-inflammatory and soothing effects; the ginger root extract has natural oxidation resistance and can help relieve damage of the external environment to the skin; ceramide can effectively supplement skin lipid and repair a damaged barrier structure. Under the synergistic effect of the bisabolol, the ginger root extract and the ceramide, uncomfortable symptoms such as redness and pruritus of sensitive skin can be quickly relieved, meanwhile, the skin barrier function is fundamentally enhanced, the skin tolerance and the water retention capacity are improved, and the composition can be applied to cosmetics as a composition for repairing the sensitive skin and strengthening the skin barrier.

Description

TECHNICAL FIELD

[0001] The present application relates to the cosmetic technology field, and in particular to a composition for repairing sensitive skin and a preparation method and application thereof. BACKGROUND

[0002] There are many factors that can induce sensitive skin, which can be divided into exogenous factors and internal factors. Exogenous factors include seasonal alternation, air pollution, ultraviolet intensity, excessive and inappropriate skin care, staying up late, stimulation of chemical substances in cosmetics, etc. Internal factors include endocrine, genetic inheritance, disease, physiological cycle, etc. The main cause of sensitive skin is the damage of skin barrier function. The damaged skin barrier function can lead to the transdermal loss of water and nutrients. The weak epidermal barrier can also enhance the penetration of irritants and allergens, inducing local inflammation of the skin, and causing symptoms such as redness, itching, burning and stinging. Properly supplementing moisturizers such as ceramides, plant oils, cholesterol, triglycerides, squalane, fatty acids, and occlusive agents can improve the skin's water retention capacity and reduce the transdermal loss of water, but can only provide immediate relief for redness and itching, and cannot effectively enhance the skin barrier function from the root. SUMMARY

[0003] The technical problem to be solved by the present application is to provide a composition for repairing sensitive skin and strengthening skin barrier, which can quickly relieve the discomfort symptoms such as redness and itching of sensitive skin, and effectively enhance the skin barrier function from the root.

[0004] The present application also aims to provide a preparation method of the composition for repairing sensitive skin and strengthening skin barrier.

[0005] The present application also aims to provide an application of the composition for repairing sensitive skin and strengthening skin barrier.

[0006] To solve the above problems, the present application discloses a composition for repairing sensitive skin and strengthening skin barrier, which comprises bisabolol, ginger root extract and ceramide, and the mass ratio of bisabolol, ginger root extract and ceramide is (0.001-5):(0.0001-1):(0.001-5).

[0007] As an improvement of the above technical solution, the ginger root extract comprises volatile ginger essential oil, non-volatile resin oil and enzymatic hydrolysate, and the mass ratio of volatile ginger essential oil, non-volatile resin oil and enzymatic hydrolysate is 1:(5-8):(1.5-3).

[0008] As an improvement of the above technical solution, the ginger root extract is prepared by the following method: The ginger root is pretreated, dried and crushed to obtain ginger powder; The ginger powder was subjected to a first CO2 supercritical extraction to obtain volatile ginger essential oil; The ginger powder was subjected to a second CO2 supercritical extraction to obtain a non-volatile resin oil; The ginger residue after CO2 supercritical extraction was enzymatically hydrolyzed to obtain the enzymatic hydrolysis product; The ginger root extract is obtained by mixing the volatile ginger essential oil, the non-volatile resin oil, and the enzymatic hydrolysis product.

[0009] As an improvement to the above technical solution, the extraction pressure of the first CO2 supercritical extraction is 80 bar to 100 bar, the extraction temperature is 35°C to 55°C, and the extraction time is 1 h to 2 h. The extraction pressure for the second CO2 supercritical extraction is 300 bar to 400 bar, the extraction temperature is 35°C to 55°C, and the extraction time is 1 h to 2 h.

[0010] As an improvement to the above technical solution, ginger residue is mixed with a buffer solution and a compound enzyme is added for enzymatic hydrolysis. The mass ratio of ginger residue to compound enzyme is 1:(0.005~0.02); The complex enzyme includes one or more of cellulase, hemicellulase, pectinase, amylase, and protease.

[0011] As an improvement to the above technical solution, the composite enzyme includes cellulase, pectinase and bromelain; the mass ratio of cellulase, pectinase and bromelain is 1:(1.5~3):(0.25~0.5); The enzyme activity of cellulase is 5000U / g~10000U / g, the enzyme activity of pectinase is 2000U / g~5000U / g, and the enzyme activity of bromelain is 1000U / g~3000U / g. The enzymatic hydrolysis time is 1h~3h, the enzymatic hydrolysis temperature is 50℃~60℃, and the enzymatic hydrolysis pH is 4.5~5.5.

[0012] As an improvement to the above technical solution, the ceramide includes one or more of ceramide NP, ceramide EOP, ceramide AS, ceramide AP, ceramide NS, and ceramide NG.

[0013] Accordingly, the present invention also discloses a method for preparing the above-mentioned composition for repairing sensitive skin and strengthening the skin barrier, comprising the following steps: Ceramide was dissolved in an oil phase at 70℃~80℃ to obtain the first component; After cooling the first component to 40℃~45℃, add the ginger extract mixture and stir well; Continue cooling to 30℃~35℃, add bisabolol, stir well, and you will get the composition for repairing sensitive skin and strengthening the skin barrier.

[0014] As an improvement to the above technical solution, the following steps are included: Ceramide was dissolved in an oil phase at 70℃~80℃ to obtain the first component; Ginger root extract was mixed with a penetration enhancer at 50℃~60℃ to obtain an activated ginger extract mixture. After cooling the first component to 40℃~45℃, add the activated ginger extract mixture and stir well; Continue cooling to 30℃~35℃, add bisabolol, stir well, and you will get the composition for repairing sensitive skin and strengthening the skin barrier.

[0015] Accordingly, the present invention also discloses the application of the above-mentioned composition for repairing sensitive skin and strengthening the skin barrier in the preparation of cosmetics, wherein the cosmetics contain 0.21wt% to 11wt% of the above-mentioned composition for repairing sensitive skin and strengthening the skin barrier.

[0016] Implementing this invention has the following beneficial effects: 1. In the composition for repairing sensitive skin and strengthening the skin barrier provided by this invention, bisabolol has anti-inflammatory and soothing effects; ginger root extract has natural antioxidant properties, which can help reduce the damage to the skin caused by the external environment; and ceramides can effectively replenish skin lipids and repair damaged barrier structures. The three work synergistically to quickly relieve discomfort symptoms such as redness and itching in sensitive skin, while strengthening the skin barrier function from the root, and improving skin tolerance and water retention capacity.

[0017] 2. This invention first performs a first CO2 supercritical extraction on ginger root to obtain volatile ginger essential oil, which promotes skin microcirculation. Then, a second CO2 supercritical extraction is performed to obtain non-volatile resin oil, which has anti-inflammatory and soothing properties and repairs the skin barrier. Finally, the ginger residue after supercritical extraction is enzymatically hydrolyzed. The peptides and polysaccharides in the enzymatic hydrolysis products have moisturizing and soothing effects, while also improving skin feel and stabilizing the composition of the ginger root extract. This invention mixes the extracted volatile ginger essential oil, non-volatile resin oil, and enzymatic hydrolysis products in a preset ratio, resulting in high utilization rate and excellent performance of the ginger root extract. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be described in further detail below.

[0019] This invention provides a composition for repairing sensitive skin and strengthening the skin barrier, comprising bisabolol, ginger root extract, and ceramide, wherein the mass ratio of bisabolol, ginger root extract, and ceramide is (0.001~5):(0.0001~1):(0.001~5). In a preferred embodiment, the mass ratio of bisabolol, ginger root extract, and ceramide is (0.01~2):(0.01~0.5):(0.01~5).

[0020] Bisabolol has anti-inflammatory and soothing effects; ginger root extract has natural antioxidant properties that can help reduce environmental damage to the skin; ceramides can effectively replenish skin lipids and repair damaged barrier structures. The synergistic effect of these three ingredients can quickly relieve discomfort symptoms such as redness and itching in sensitive skin, while simultaneously strengthening the skin barrier function from the root, improving skin tolerance and moisture retention.

[0021] In one embodiment, the ginger root extract comprises volatile ginger essential oil, non-volatile resin oil, and enzymatic hydrolysis product, wherein the mass ratio of the volatile ginger essential oil, non-volatile resin oil, and enzymatic hydrolysis product is 1:(5~8):(1.5~3), exemplarily 1:5:3, 1:8:1.5, 1:6:2, 1:6:3, or 1:7:2, but is not limited thereto.

[0022] Ginger essential oil is a volatile aromatic substance, and its core function comes from terpenes such as gingerene. Ginger essential oil can open up channels for other active ingredients, significantly enhancing the overall efficacy of the product; secondly, it can stimulate skin microcirculation, improve facial complexion, and relieve muscle tension; in addition, it has natural antibacterial properties, which helps the product's preservative system and is beneficial for acne-prone skin.

[0023] Gingerol resin oil is a highly concentrated fat-soluble component, specifically gingerol and its derivatives. It is a potent natural antioxidant that neutralizes free radicals, combats oxidative stress, and delays photoaging of the skin. Simultaneously, it possesses anti-inflammatory and soothing properties, effectively inhibiting the release of pro-inflammatory factors, thereby reducing skin redness, irritation, and sensitivity. It is particularly beneficial for soothing irritated skin and improving symptoms such as rosacea. Furthermore, it can inhibit tyrosinase activity, thus having a certain auxiliary whitening effect.

[0024] Enzymatic hydrolysis products are derived from the resource utilization of ginger residue, and their main components include ginger polysaccharides, peptides, and amino acids. Ginger polysaccharides have good moisturizing abilities, preventing moisture loss; at the same time, they have soothing and repairing properties, reducing skin inflammation and promoting barrier repair. Furthermore, the small-molecule peptides and amino acids produced by enzymatic hydrolysis are easily absorbed by the skin, providing nutrition and helping to maintain skin hydration and health. The enzymatic hydrolysis products have a gentle texture and can effectively buffer the potential irritation from ginger essential oil and resin oil.

[0025] This invention limits the specific components and mass ratios of ginger root extract. The penetration-enhancing effect of ginger essential oil helps the core active ingredients in resin oil and the soothing polysaccharides in enzymatic hydrolysates to penetrate more deeply. Resin oil provides powerful antioxidant and anti-inflammatory effects. The polysaccharides and peptides in the enzymatic hydrolysates not only moisturize and repair but also buffer the potential irritation from ginger essential oil and resin oil. The resulting ginger root extract is suitable for moisturizing and soothing products for sensitive skin, achieving a balance between gentleness and efficacy. If the content of volatile ginger essential oil is too high, it may exacerbate irritation such as burning, stinging, and redness, and an overly pungent odor may cause discomfort to consumers. If the content of non-volatile resin oil is too high, the product will have a sticky texture, affecting the user experience, and excessive oil-soluble components may clog pores and increase the burden on the skin. If the enzymatic hydrolysates are too high, it will hinder the enhancement of the product's antioxidant and anti-inflammatory effects and also impede the penetration of ginger essential oil and resin oil.

[0026] In one embodiment, the ginger root extract is prepared by the following method: S11. Pre-treat ginger roots, dry them, and then pulverize them to obtain ginger powder.

[0027] Specifically, ginger is cleaned by removing the roots and leaves, sliced, and dried at 20℃~45℃ for 24h~96h. The dried ginger slices are then pulverized and passed through a 100-200 mesh sieve to obtain ginger powder. Alternatively, ginger can be dried using low-temperature freeze-drying or low-temperature vacuum drying.

[0028] S12. The ginger powder is subjected to a first CO2 supercritical extraction to obtain volatile ginger essential oil.

[0029] Specifically, the first CO2 supercritical extraction is carried out under the combined action of liquid CO2 and an entrainer, which is 85wt%~95wt% ethanol. The extraction pressure is 80bar~100bar, the extraction temperature is 35℃~55℃, and the extraction time is 1h~2h. Operating at a lower pressure preferentially extracts volatile ginger essential oil.

[0030] S13. The ginger powder is subjected to a second CO2 supercritical extraction to obtain a non-volatile resin oil.

[0031] Specifically, the second CO2 supercritical extraction is carried out under the combined action of liquid CO2 and an entrainer, which is 85wt%~95wt% ethanol. The extraction pressure is 300bar~400bar, the extraction temperature is 35℃~55℃, and the extraction time is 1h~2h. Increasing the pressure extracts non-volatile resin oils such as gingerol.

[0032] In a preferred embodiment, the entrainer is ethanol and a eutectic solvent in a mass ratio of 1:(0.05~0.15), wherein the mass fraction of ethanol is 85%~95%, and the eutectic solvent includes choline chloride and glycerol in a molar ratio of 1:(1.8~2.2). In the resin oil, gingerol has phenolic hydroxyl groups; glycerol, rich in hydroxyl groups, is selected to form strong hydrogen bonds with gingerol, achieving directional enrichment, improving the purity of gingerol in the extract, and reducing impurities.

[0033] S14. The ginger residue after CO2 supercritical extraction is enzymatically hydrolyzed to obtain the enzymatic hydrolysis product.

[0034] Specifically, ginger residue is mixed with a buffer solution, and a compound enzyme is added for enzymatic hydrolysis. It is understood that the buffer solution can be deionized water, or a dilute acid solution such as citric acid or hydrochloric acid, or a dilute alkaline solution such as sodium hydroxide; no specific limitation is made here. After enzymatic hydrolysis, enzyme inactivation is performed, followed by crushing and drying. Specifically, enzyme inactivation can be achieved through heat treatment, pH adjustment, or organic solvent precipitation. This invention preferably uses heat treatment at 80℃~90℃ for 5~8 minutes.

[0035] The mass ratio of ginger residue to the compound enzyme is 1:(0.005~0.02), exemplarily 1:0.008, 1:0.01, 1:0.012, 1:0.015, or 1:0.018, but not limited to these. Excessive or insufficient addition of the compound enzyme will reduce the enzymatic hydrolysis efficiency. Specifically, the hydrolysis time is 1h~3h, the hydrolysis temperature is 50℃~60℃, and the hydrolysis pH is 4.5~5.5.

[0036] Optionally, the complex enzyme includes one or more of cellulase, hemicellulase, pectinase, amylase, and protease. In one embodiment, the complex enzyme includes cellulase, pectinase, and bromelain, wherein the mass ratio of cellulase, pectinase, and bromelain is 1:(1.5~3):(0.25~0.5), exemplary ratios are 1:1.5:0.5, 1:3:0.25, 1:2:0.5, 1:2:0.3, or 1:2.5:0.35, but is not limited thereto. More preferably, the mass ratio of cellulase, pectinase, and bromelain is 1:(2~3):(0.3~0.45).

[0037] Pectinase hydrolyzes pectin between cells, allowing cellulase and bromelain to more easily and deeply contact and attack their respective substrates, significantly improving overall enzymatic hydrolysis efficiency.

[0038] Cellulase hydrolyzes cellulose and hemicellulose, breaking down the insoluble fibers of the robust cell wall skeleton into functional polysaccharides.

[0039] Bromelain efficiently hydrolyzes residual proteins in the residue, generating small molecule peptides and amino acids, which have antioxidant and osmotic-enhancing biological activities, thus enhancing the efficacy of the final product.

[0040] Through the synergistic effect of three enzymes, functionally rich enzymatic hydrolysates can be obtained from ginger residue, which have excellent moisturizing, film-forming and soothing effects, as well as certain antioxidant capacity.

[0041] In a preferred embodiment, the cellulase activity is 5000 U / g to 10000 U / g, the pectinase activity is 2000 U / g to 5000 U / g, and the bromelain activity is 1000 U / g to 3000 U / g. The structure of the ginger residue can be effectively disrupted, significantly increasing sugar yield and protein hydrolysis degree.

[0042] S15. The volatile ginger essential oil, non-volatile resin oil and enzymatic hydrolysis product are mixed to obtain the ginger root extract.

[0043] This invention first performs a first CO2 supercritical extraction on ginger root to obtain volatile ginger essential oil, which promotes skin microcirculation. Then, a second CO2 supercritical extraction is performed to obtain non-volatile resin oil, which has anti-inflammatory and soothing properties and repairs the skin barrier. Finally, the ginger residue after supercritical extraction is enzymatically hydrolyzed. The peptides and polysaccharides in the enzymatic hydrolysis products have moisturizing and soothing effects, while also improving skin feel and stabilizing the composition of the ginger root extract. The extraction method provided by this invention yields ginger root extract with high utilization rate and good stability.

[0044] In one embodiment, the ceramide includes one or more of ceramide NP, ceramide EOP, ceramide AS, ceramide AP, ceramide NS, and ceramide NG.

[0045] Optionally, bisabolol can be obtained by extraction from plants such as German chamomile and Brazilian immortelle, or by biotransformation using genetically engineered yeast or bacteria; no specific limitation is made here.

[0046] Accordingly, the present invention also discloses a method for preparing the above-mentioned composition for repairing sensitive skin and strengthening the skin barrier, comprising the following steps: S21. Ceramide is dissolved in an oil phase at 70℃~80℃ to obtain the first component.

[0047] High-temperature oil dissolution ensures complete dispersion of ceramides, preventing later crystallization and precipitation that could affect product stability and skin feel. The oil phase can be conventional oil phases such as phytosterols or glycerides; no specific limitations are specified here.

[0048] S22. After cooling the first component to 40℃~45℃, add the ginger extract mixture and stir well.

[0049] After cooling, ginger root extract is added. However, gingerol in the ginger root extract will convert to shogaol at high temperatures. Shogaol is highly irritating and not suitable for consumers with damaged skin barriers, extreme sensitivity, or skin inflammation. Furthermore, the conversion process can cause the cosmetic's color to darken and its odor to change, leading to unstable quality.

[0050] S23. Continue cooling to 30℃~35℃, add bisabolol, stir evenly, and you will get the composition for repairing sensitive skin and strengthening the skin barrier.

[0051] Bisabolol has a certain degree of volatility and heat sensitivity. Continuing to cool down and adding it last at a low temperature can maximize the preservation of its activity and stability.

[0052] By strictly controlling the degree of cooling, improving the uniformity of the system, and avoiding the deactivation of active ingredients in the composition, the efficacy and stability of the final cosmetic product are superior to those of a simple mixture.

[0053] In a preferred embodiment, a method for preparing a composition for repairing sensitive skin and strengthening the skin barrier includes the following steps: S21. Ceramide is dissolved in an oil phase at 70℃~80℃ to obtain the first component.

[0054] S22. Ginger root extract and penetration enhancer are mixed at 50℃~60℃ to obtain activated ginger extract mixture.

[0055] Preheating and pre-mixing ginger root extract with a penetration enhancer can stabilize its active ingredients and form a complex with the penetration enhancer, thereby enhancing skin permeability. The penetration enhancer can be lecithin, etc., and is not specifically limited here.

[0056] S23. After cooling the first component to 40℃~45℃, add the activated ginger extract mixture and stir until homogeneous.

[0057] S24. Continue cooling to 30℃~35℃, add bisabolol, stir evenly, and you will get the composition for repairing sensitive skin and strengthening the skin barrier.

[0058] Accordingly, this invention also discloses the application of the above-mentioned composition for repairing sensitive skin and strengthening the skin barrier in the preparation of cosmetics, wherein the cosmetics contain 0.21wt% to 11wt% of the above-mentioned composition for repairing sensitive skin and strengthening the skin barrier. The remaining ingredients are excipients, which are commercially available excipients conventional in the art. Preferably, the cosmetics contain 0.5wt% to 5wt% of the above-mentioned composition for repairing sensitive skin and strengthening the skin barrier.

[0059] The present invention will be further described below with reference to specific embodiments: Example 1 This embodiment provides a composition for repairing sensitive skin and strengthening the skin barrier, comprising bisabolol, ginger root extract and ceramide, wherein the mass ratio of bisabolol, ginger root extract and ceramide is 1:0.1:1.

[0060] Both bisabolol and ceramide are commercially available.

[0061] The ginger root extract was prepared by the following method: dried ginger root slices were extracted with 1,3-propanediol at 70°C for 2 hours. The extract was then filtered through 5 μm and 0.22 μm polypropylene membranes to obtain the ginger root extract.

[0062] A method for preparing a composition for repairing sensitive skin and strengthening the skin barrier includes the following steps: S21. Ceramide is dissolved in the oil phase at 75°C to obtain the first component.

[0063] S22. After cooling the first component to 45°C, add the ginger extract mixture and stir until well mixed.

[0064] S23. Continue cooling to 35℃, add bisabolol, stir well, and you will get a composition for repairing sensitive skin and strengthening the skin barrier.

[0065] Example 2 This embodiment provides a composition for repairing sensitive skin and strengthening the skin barrier. The difference between this composition and that of Example 1 is that the mass ratio of bisabolol, ginger root extract and ceramide is 1:0.5:2.

[0066] Everything else is the same as in Example 1.

[0067] Example 3 This embodiment provides a composition for repairing sensitive skin and strengthening the skin barrier. The difference between this composition and that of Example 2 is that the ginger root extract includes volatile ginger essential oil, non-volatile resin oil and enzymatic hydrolysate, and the mass ratio of volatile ginger essential oil, non-volatile resin oil and enzymatic hydrolysate is 1:6:2.

[0068] Ginger root extract was prepared using the following method: S11. Pre-treat ginger roots, dry them, and then pulverize them to obtain ginger powder.

[0069] S12. Ginger powder was subjected to a first CO2 supercritical extraction to obtain volatile ginger essential oil. The extraction pressure of the first CO2 supercritical extraction was 90 bar, the extraction temperature was 45℃, and the extraction time was 1.5 h.

[0070] S13. Ginger powder was subjected to a second supercritical CO2 extraction to obtain a non-volatile resin oil. The extraction pressure of the second supercritical CO2 extraction was 350 bar, the extraction temperature was 45℃, and the extraction time was 1.5 h.

[0071] S14. The ginger residue after CO2 supercritical extraction was subjected to enzymatic hydrolysis to obtain the enzymatic hydrolysis product. Cellulase was used for enzymatic hydrolysis, the mass ratio of ginger residue to cellulase was 1:0.01, the hydrolysis time was 1.5 h, the hydrolysis temperature was 55 ℃, and the hydrolysis pH was 5.

[0072] S15. Mix volatile ginger essential oil, non-volatile resin oil and enzymatic hydrolysis products to obtain ginger root extract.

[0073] Everything else is the same as in Example 2.

[0074] Example 4 This embodiment provides a composition for repairing sensitive skin and strengthening the skin barrier. The difference between this composition and that of Example 3 is that the enzymatic hydrolysis uses a complex enzyme, which includes cellulase, pectinase and bromelain, and the mass ratio of cellulase, pectinase and bromelain is 1:2:0.3.

[0075] Everything else is the same as in Example 3.

[0076] Example 5 This embodiment provides a composition for repairing and strengthening the skin barrier of sensitive skin. The difference between this embodiment and Example 4 is that the preparation method of the composition for repairing and strengthening the skin barrier of sensitive skin includes the following steps: S21. Ceramide is dissolved in the oil phase at 75°C to obtain the first component.

[0077] S22. Ginger root extract and penetration enhancer are mixed at 55°C to obtain an activated ginger extract mixture.

[0078] S23. After cooling the first component to 40°C, add the activated ginger extract mixture and stir until homogeneous.

[0079] S24. Continue cooling to 35℃, add bisabolol, stir well, and you will get a composition for repairing sensitive skin and strengthening the skin barrier.

[0080] Everything else is the same as in Example 4.

[0081] The compositions provided in Examples 1 and 5 were mixed with excipients to prepare cosmetics, wherein the mass percentage of the compositions was 1%, resulting in Application Example 1 and Application Example 2. The compositions provided in Examples 1 and 5 were mixed with excipients to prepare cosmetics, wherein the mass percentage of the compositions was 5%, resulting in Application Example 3 and Application Example 4.

[0082] Performance testing: (1) Evaluation of the antibacterial efficacy of the composition (1.1) Under aseptic conditions, *Propionibacterium acnes* and *Staphylococcus aureus* were inoculated onto slant agar using the streak method and incubated at 37°C for 24 hours. Nutrient agar and liquid culture medium were autoclaved, cooled, and poured into sterilized petri dishes. The activated bacterial strains were inoculated into sterilized liquid culture medium using an inoculation loop and incubated at 37°C for 24 hours. The total bacterial count of the suspension was adjusted to 1 × 10⁻⁶ using 0.9% physiological saline. 6 CFU / mL ~ 1×10 7 CFU / mL.

[0083] (1.2) Accurately pipette 100L of bacterial suspension and add it to the solid culture medium. Spread it evenly and use sterilized tweezers to pick up the round filter paper containing the sample solutions of Examples 1 to 5 and place it on the petri dish. The diameter of the round filter paper is 6mm. Incubate in a constant temperature incubator at 37℃ for 24h. Take it out and measure the diameter of the inhibition zone with vernier calipers. Perform three parallel measurements and take the average value.

[0084] (1.3) The antibacterial effect of the sample is determined based on the diameter of the inhibition zone. The criteria are as follows: inhibition zone diameter > 20 mm indicates extremely sensitive, 15 mm < inhibition zone diameter ≤ 20 mm indicates highly sensitive, 9 mm < inhibition zone diameter ≤ 15 mm indicates moderately sensitive, 7 mm < inhibition zone diameter ≤ 9 mm indicates lowly sensitive, and no inhibition zone indicates no sensitivity.

[0085] The specific results are shown in the table below:

[0086] (2) Evaluation of the soothing efficacy of the composition Hyaluronidase activity inhibition assay: Prepare four 10mL stoppered test tubes, named A, B, C, and D respectively. Add 0.5mL of sample solution to A and B respectively, and 0.5mL of deionized water to C and D respectively. Then add 0.5mL of hyaluronidase solution to A and C respectively, and 0.5mL of acetate buffer to B and D respectively. Place A, B, C, and D together in an oven and incubate at 37℃ for 20min. Add 0.25mmol·L⁻¹ of hyaluronidase solution to A, B, C, and D respectively. -1 After adding 0.1 mL of sodium chloride solution, place it in an oven and keep it at 37°C for 20 min; then add 0.5 mg·mL⁻¹ to A and C respectively. -12 mL of sodium hyaluronate was added to each of the following solutions: B and D. 2 mL of acetate buffer was added to each solution, and the solutions were placed in an oven at 37°C for 20 min. After incubation, the solutions were removed and allowed to stand at room temperature for 10 min. Then, 1 mL of acetylacetone solution was added to each of the following solutions. The solutions were reacted in a water bath at boiling temperature for 15 min, and then immediately placed in an ice-water bath for 15 min. After standing at room temperature for 10 min, Ehrlich chromogenic reagent was added to each of the following solutions and the mixture was shaken thoroughly. The solutions were then diluted with 3.0 mL of anhydrous ethanol and allowed to stand at room temperature for 40 min. The absorbance was measured at 520 nm using a UV-Vis spectrophotometer. The hyaluronidase activity inhibition rate was calculated using the following formula:

[0087] In the formula, OD A OD B OD C OD D The absorbance values ​​at 520 nm are shown in the table below for the solutions in test tubes A, B, C, and D, respectively. The specific results are shown in the table below:

[0088] (3) Evaluation of the repair efficacy of cosmetics Thirty subjects with normal skin (15 men and 15 women, aged 18-50 years) were tested in an environment of 25±2℃ and 60% humidity. All subjects washed their arms with water and waited for 30 minutes. Five test areas were selected on the inner forearms of both subjects and marked. 20 μL of 1% histamine solution was applied to a patch and adhered to the test area. After 30 minutes, the patch was removed, and samples corresponding to Application Examples 1-4 were evenly applied to the test area using a latex finger cot. The test area without any sample was the blank group. The time for the erythema and itching to subside at the patch location was recorded. Specific results are shown in the table below:

[0089] The above description is a preferred embodiment of the invention. It should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the principle of the invention, and these improvements and modifications are also considered to be within the scope of protection of the invention.

Claims

1. A composition for repairing the barrier of sensitive skin, characterized in that it comprises, The composition comprises bisabolol, ginger extract and ceramide, and the mass ratio of the bisabolol, ginger extract and ceramide is (0.001-5):(0.0001-1):(0.001-5).

2. The composition for repairing the skin barrier of sensitive skin to strengthen the skin of claim 1, wherein The ginger extract comprises volatile ginger essential oil, non-volatile resin oil and enzymatic hydrolysate, and the mass ratio of the volatile ginger essential oil, non-volatile resin oil and enzymatic hydrolysate is 1:(5-8):(1.5-3).

3. The composition for repairing the skin barrier of sensitive skin to strengthen the skin of claim 1, wherein The ginger extract is prepared by the following method: The ginger is pretreated, dried and then crushed to obtain ginger powder; The ginger powder is subjected to first CO2 supercritical extraction to obtain volatile ginger essential oil; The ginger powder is subjected to second CO2 supercritical extraction to obtain non-volatile resin oil; The ginger residue after CO2 supercritical extraction is subjected to enzymatic hydrolysis to obtain enzymatic hydrolysate; The volatile ginger essential oil, non-volatile resin oil and enzymatic hydrolysate are mixed to obtain the ginger extract.

4. The composition for repairing the skin barrier of sensitive skin to strengthen the skin of claim 3, wherein The extraction pressure of the first CO2 supercritical extraction is 80-100 bar, the extraction temperature is 35-55 DEG C, and the extraction time is 1-2 h; The extraction pressure of the second CO2 supercritical extraction is 300-400 bar, the extraction temperature is 35-55 DEG C, and the extraction time is 1-2 h.

5. The composition for repairing the skin barrier of sensitive skin to strengthen the skin of claim 3, wherein The ginger residue is mixed with a buffer solution, and a composite enzyme is added for enzymatic hydrolysis; The mass ratio of the ginger residue to the composite enzyme is 1:(0.005-0.02); The composite enzyme comprises one or more of cellulase, hemicellulase, pectinase, amylase and protease.

6. The composition for repairing the skin barrier of sensitive skin to strengthen the skin of claim 5, wherein The composite enzyme comprises cellulase, pectinase and bromelain, and the mass ratio of the cellulase, pectinase and bromelain is 1:(1.5-3):(0.25-0.5); The enzyme activity of the cellulase is 5000-10000 U / g, the enzyme activity of the pectinase is 2000-5000 U / g, and the enzyme activity of the bromelain is 1000-3000 U / g; The enzymatic hydrolysis time is 1-3 h, the enzymatic hydrolysis temperature is 50-60 DEG C, and the enzymatic hydrolysis pH is 4.5-5.

5.

7. The composition for repairing the skin barrier of sensitive skin to strengthen the skin of claim 1, wherein The ceramide comprises one or more of ceramide NP, ceramide EOP, ceramide AS, ceramide AP, ceramide NS and ceramide NG.

8. A method of preparing the composition for repairing the barrier of sensitive skin to strengthen the skin barrier according to any one of claims 1 to 7, characterized in that, The method comprises the following steps: The ceramide is dissolved in an oil phase at 70-80 DEG C to obtain a first component; After the first component is cooled to 40-45 DEG C, the ginger extract mixture is added and stirred uniformly; The temperature is continuously lowered to 30-35 DEG C, the bisabolol is added and stirred uniformly to obtain the composition for repairing sensitive skin and strengthening skin barrier.

9. The method for preparing the composition for repairing sensitive skin and strengthening the skin barrier as described in claim 8, characterized in that, The method comprises the following steps: The ceramide is dissolved in an oil phase at 70-80 DEG C to obtain a first component; The ginger extract is mixed with a penetration aid at 50-60 DEG C to obtain an activated ginger extract mixture; After the first component is cooled to 40-45 DEG C, the activated ginger extract mixture is added and stirred uniformly; The temperature is continuously lowered to 30-35 DEG C, the bisabolol is added and stirred uniformly to obtain the composition for repairing sensitive skin and strengthening skin barrier.

10. Use of the composition for repairing the barrier of sensitive skin according to any one of claims 1 to 7, in the manufacture of a cosmetic product, characterized in that, The cosmetic contains 0.21wt%~11wt% of the composition for repairing sensitive skin barrier and strengthening skin barrier as claimed in any one of claims 1~7. The cosmetic contains 0.21wt%~11wt% of the composition for repairing sensitive skin barrier and strengthening skin barrier as claimed in any one of claims 1~7.

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