A mild cleansing composition and its use

Abstract

The application discloses a mild cleaning composition and application thereof, and belongs to the technical field of cosmetics. The mild cleaning composition comprises sodium lauroyl glutamate, decyl glucoside, Tween-28, cocamidopropyl betaine, plant saponin and ikkduin. The plant saponin comprises camellia seed extract, and further comprises at least one of acanthopanax extract, soapberry extract and gynostemma extract. The mild cleaning composition is prepared by selecting suitable components, and the components have good interaction. When the mild cleaning composition is applied to the preparation of a washing and protecting product, the mild cleaning composition has good surface activity, can effectively clean the skin, is very mild, does not cause skin dryness and tightness after washing, does not affect the skin barrier function, and has low residue after use, thereby avoiding continuous stimulation to the skin.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, and in particular relates to a mild cleansing composition and its application. Background Technology

[0002] Infants' skin has a stratum corneum that is only one-third the thickness of an adult's, with less sebum secretion and an immature barrier function, making it susceptible to external stimuli that can lead to dryness, itching, and inflammation. Furthermore, patients with atopic dermatitis (AD) are particularly concerned about the gentleness of bath products, as improper use may worsen skin problems. The Chinese Guidelines for the Diagnosis and Treatment of Atopic Dermatitis (2020 Edition) state that proper bathing can remove dirt and crusts from the skin surface and recommends the use of hypoallergenic and non-irritating cleansing products.

[0003] Surfactants are the main ingredients in bath and body products. The structure and type of surfactants determine their irritation to the skin, and improper selection may cause various skin problems.

[0004] In pursuit of foaming performance and cleaning power, current technologies select surfactants with good foaming properties, which excessively strip away lipids from the skin's surface, leading to dry skin after washing. Existing technologies often use compound surfactants and added polymers to reduce product irritation, neglecting the issue of surfactant residue on the skin's surface. Furthermore, they emphasize gentleness while ignoring the problems inherent in problematic skin types, resulting in shampoo and body wash products focusing solely on cleansing while neglecting skin protection. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide a mild cleansing composition and its application that has good surface activity and excellent cleaning ability, as well as excellent gentleness, does not cause dry and tight skin after washing, does not affect the skin barrier function, and leaves little residue.

[0006] To achieve the above objectives, in a first aspect of the present invention, the present invention provides a mild cleaning composition comprising sodium lauroyl glutamate, decyl glucoside, Tween-28, cocamidopropyl betaine, plant saponins, and ectoine.

[0007] The plant saponins include camellia seed extract, and also include at least one of Acanthopanax senticosus extract, Sapindus mukorossi extract and Gynostemma pentaphyllum extract.

[0008] The present invention has discovered that the gentle cleansing composition provided by the present invention, through the selection and compounding of appropriate types of components, exhibits good interactions between the components. When subsequently applied to the preparation of personal care products, it not only has good surface activity, effectively cleansing the skin, but also has excellent moisturizing and soothing effects without affecting the skin barrier function. Furthermore, the resulting personal care products have low residue after use, avoiding continuous irritation to the skin, and are suitable for all people, especially infants and Alzheimer's disease patients.

[0009] Specifically, sodium lauroyl glutamate is an amino acid-based surfactant with good mildness and excellent cleaning ability, effectively removing dirt. In the system of this invention, it synergistically reduces skin residue from other components, further enhancing the product's mildness. Decyl glucoside is a nonionic surfactant with a short carbon chain. In the subsequent preparation of washing and care products, it promotes micelle formation in this system, intercalating with other surfactant molecules to form larger micelle structures, reducing the irritation of free surfactant molecules on the skin and improving the product's mildness. Furthermore, under the action of sodium lauroyl glutamate, the prepared washing and care products are easy to rinse and have low residue, further enhancing the product's mildness. Tween-28 is also a nonionic surfactant that can interact with other surfactants in the composition, reducing the irritation of the surfactant composition, synergistically improving the mildness and viscosity of the surfactant composition, and reducing the amount of thickener used in the system. Cocamidopropyl betaine has good foaming ability in the surfactant system provided by this invention and can effectively reduce its residue on the skin surface under the surfactant combination. Ectoin is a natural amino acid derivative that, in the system of this invention, exhibits excellent moisturizing and water-locking effects as well as strengthening the skin barrier function. It also possesses certain anti-inflammatory, soothing, and antioxidant properties, synergistically reducing the irritation of the composition. The natural surfactant plant saponins selected in this invention can synergistically achieve excellent cleansing effects with other surfactants, reducing the amount of synthetic surfactants used and further minimizing surfactant residue on the skin surface. Furthermore, they can synergistically enhance the moisturizing and soothing capabilities of the gentle cleansing composition and improve skin barrier function. More specifically, camellia seed extract contains polyphenols and polysaccharides in addition to saponins, providing moisturizing, soothing, and antibacterial effects; Acanthopanax senticosus extract contains polyphenols and polysaccharides in addition to saponins, offering good moisturizing and soothing effects in addition to its cleansing ability, relieving dry skin after cleansing; Sapindus mukorossi extract contains saponins, widely used in cleansing, and also contains flavonoids and polysaccharides, effectively relieving dry skin after cleansing; Gynostemma pentaphyllum extract contains flavonoids, amino acids, and polysaccharides in addition to saponins, providing moisturizing and soothing effects.

[0010] As a preferred embodiment of the mild cleaning composition of the present invention, the mild cleaning composition comprises the following components in parts by weight: 0.5-8 parts sodium lauroyl glutamate, 1-8 parts decyl glucoside, 0.5-8 parts Tween-28, 0.5-2.5 parts cocamidopropyl betaine, 0.05-5 parts plant saponins, and 0.05-2 parts ectoine.

[0011] As a preferred embodiment of the mild cleaning composition of the present invention, the mild cleaning composition comprises the following components in parts by weight: 5-6 parts sodium lauroyl glutamate, 3-5 parts decyl glucoside, 3-6 parts Tween-28, 1-2 parts cocamidopropyl betaine, 1.5-3 parts plant saponins, and 1-1.5 parts ectoine.

[0012] This invention has found that the amount of each component added to a mild cleansing composition affects the interaction between the components, thereby affecting the surface activity, the effect on the skin barrier, and the skin moisture content of the resulting cleansing product after the mild cleansing composition is applied. It also affects the mildness of the cleansing product prepared subsequently. When the mass fraction of the components in the mild cleansing composition is further selected within the above range, the overall performance of the resulting product is better.

[0013] As a preferred embodiment of the mild cleaning composition of the present invention, the plant saponins include camellia seed extract, Acanthopanax senticosus extract and Gynostemma pentaphyllum extract, wherein the mass ratio of the camellia seed extract, Acanthopanax senticosus extract and Gynostemma pentaphyllum extract is 1:(0.2-0.8):(0.1-0.6).

[0014] The present invention has found that further selection of plant saponins, including camellia seed extract, Acanthopanax senticosus extract and Gynostemma pentaphyllum extract, into the system of the present invention, and especially when the mass ratio of the three is within the above-mentioned range, results in a mild cleaning composition with even better overall effects.

[0015] For example, the mass ratio of the camellia seed extract, Acanthopanax senticosus extract, and Gynostemma pentaphyllum extract can be any point value or any two points within the range of 1:(0.2-0.8):(0.1-0.6), such as 1:0.2:0.1, 1:0.2:0.3, 1:0.2:0.6, 1:0.4:0.1, 1:0.4:0.3, 1:0.4:0.6, 1:0.6:0.1, 1:0.6:0.3, 1:0.6:0.6, 1:0.8:0.1, 1:0.8:0.3, 1:0.8:0.6, etc.

[0016] As a preferred embodiment of the mild cleaning composition of the present invention, the mass percentage of ectoine is ≥0.29% based on the total mass of the mild cleaning composition.

[0017] Preferably, the ectoine comprises 0.29-10.5% by mass of the total mass of the mild cleaning composition.

[0018] For example, the mass percentage of ectoine, based on the total mass of the mild cleaning composition, can be any point value or any two-point range between 0.29% and 10.5%, such as 0.29%, 0.32%, 0.4%, 0.6%, 0.8%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, 10.5%, etc.

[0019] More preferably, the ectoine comprises 6-7% by mass of the total mass of the mild cleaning composition.

[0020] This invention has found that the mass percentage of ectoine in a mild cleansing composition not only affects the moisturizing properties and skin barrier function enhancement of the subsequent shampoo and body care products prepared from the mild cleansing composition, but also affects the surface activity and mildness of the products. When the mass percentage of ectoine in the mild cleansing composition is further selected to be 0.29-10.5%, especially 6-7%, the overall performance of the shampoo and body care products prepared from the mild cleansing composition is better.

[0021] As a preferred embodiment of the mild cleaning composition of the present invention, the mass percentage of cocamidopropyl betaine is ≥2.62% based on the total mass of the mild cleaning composition.

[0022] Preferably, the mass percentage of cocamidopropyl betaine is 2.62-15% based on the total mass of the mild cleaning composition.

[0023] For example, the mass percentage of cocamidopropyl betaine, based on the total mass of the mild cleaning composition, can be any point value or any two-point range between 2.62% and 15%, such as 2.62%, 2.8%, 3.0%, 3.2%, 3.4%, 3.6%, 3.8%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, etc.

[0024] More preferably, the mass percentage of cocamidopropyl betaine is 7.5-8.5% based on the total mass of the mild cleaning composition.

[0025] This invention has found that the mass percentage of cocamidopropyl betaine in a mild cleansing composition affects its interaction with surfactants, thus influencing the foaming effect of the subsequently prepared shampoo and body care products. This not only affects the cleansing ability but also the user's experience. Furthermore, it affects the amount of residue left on the skin surface, thereby impacting the product's gentleness and its ability to protect the skin barrier. When the mass percentage of cocamidopropyl betaine is further selected within the aforementioned range, the resulting product exhibits superior overall performance.

[0026] As a preferred embodiment of the mild cleaning composition of the present invention, the ratio of the sum of the mass of the plant saponins and decyl glucoside to the sum of the mass of sodium lauroyl glutamate and Tween-28 is ≥0.4.

[0027] Preferably, the ratio of the sum of the mass of the plant saponins and decyl glucoside to the sum of the mass of sodium lauroyl glutamate and Tween-28 is 0.4-0.9.

[0028] For example, the ratio of the sum of the mass of the plant saponins and decyl glucoside to the sum of the mass of sodium lauroyl glutamate and Tween-28 can be any point value or any two points between 0.4 and 0.9, such as 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, etc.

[0029] More preferably, the ratio of the sum of the mass of the plant saponins and decyl glucoside to the sum of the mass of sodium lauroyl glutamate and Tween-28 is 0.6-0.7.

[0030] This invention has found that the ratio of the sum of the masses of plant saponins and decyl glucoside to the sum of the masses of sodium lauroyl glutamate and Tween-28 not only affects the surface activity of the mild cleansing composition, but also its ability to increase skin moisture content and protect the skin barrier. When the ratio of the sum of the masses of plant saponins and decyl glucoside to the sum of the masses of sodium lauroyl glutamate and Tween-28 is further selected to be 0.4-0.9, especially 0.6-0.7, the resulting mild cleansing composition exhibits superior overall performance.

[0031] As a preferred embodiment of the mild cleansing composition of the present invention, the method for preparing the camellia seed extract includes the following steps:

[0032] (1) Add the dried and pulverized camellia seeds to deionized water, and then add a compound enzyme preparation for enzymatic hydrolysis and enzyme inactivation;

[0033] (2) Add deionized water to the enzyme hydrolysate after enzyme inactivation treatment and extract by ultrasonication. After extraction, separate solid and liquid, collect the liquid and concentrate and dry it to obtain camellia seed extract.

[0034] In step (1), the compound enzyme preparation includes cellulase and papain.

[0035] This invention has found that the camellia seed extract prepared using the method provided by this invention, when applied to a mild cleansing composition, can enhance the cleansing effect and gentleness of the composition without affecting the skin barrier function. Furthermore, the method for preparing the camellia seed extract provided by this invention is environmentally friendly.

[0036] As a preferred embodiment of the mild cleaning composition of the present invention, in step (1), the mass ratio of cellulase to papain is 2:(0.5-1.5).

[0037] Preferably, in step (1), the mass ratio of cellulase to papain is 2:1.

[0038] In a preferred embodiment of the mild cleaning composition of the present invention, in step (1), the mass percentage of the compound enzyme preparation is 2-4% based on the mass of camellia seeds.

[0039] As a preferred embodiment of the mild cleaning composition of the present invention, in step (1), the enzymatic hydrolysis temperature is 50±5℃, the enzymatic hydrolysis time is 90-150min, and the enzymatic hydrolysis pH value is 4.5-5.5.

[0040] Preferably, in step (1), the enzymatic hydrolysis temperature is 50±2℃, the enzymatic hydrolysis time is 110-130min, and the enzymatic hydrolysis pH value is 4.8-5.2.

[0041] It should be noted that the enzymatic hydrolysis pH value was obtained by adjusting the citric acid / sodium citrate buffer system.

[0042] In a preferred embodiment of the mild cleaning composition of the present invention, in step (1), the mass ratio of camellia seeds to deionized water is 1:(10-20).

[0043] Preferably, in step (1), the mass ratio of camellia seeds to deionized water is 1:15.

[0044] In a preferred embodiment of the mild cleaning composition of the present invention, in step (1), the enzyme inactivation temperature is 90±5℃ and the enzyme inactivation time is 25-35min.

[0045] In a preferred embodiment of the mild cleaning composition of the present invention, in step (2), the ultrasonic extraction frequency is 20-40 kHz and the ultrasonic time is 40-60 min.

[0046] Preferably, in step (2), the ultrasonic frequency of ultrasonic extraction is 28-32kHz and the ultrasonic time is 48-52min.

[0047] In a preferred embodiment of the mild cleaning composition of the present invention, in step (2), when adding deionized water to the enzymatic hydrolysate after enzyme inactivation treatment for ultrasonic extraction, the volume ratio of deionized water to enzymatic hydrolysate is (0.6-1):1.

[0048] In a preferred embodiment of the mild cleaning composition of the present invention, the ultrasonic extraction temperature in step (2) is 35-45°C.

[0049] In a second aspect, the present invention provides the use of the mild cleaning composition in the preparation of personal care products.

[0050] The mild cleaning composition provided by this invention has good surface activity, excellent foaming ability, and strong cleaning power; it also has low residue and high gentleness; at the same time, it can effectively increase the moisture content of the skin after use without affecting the skin barrier function; thus, it can be widely used in washing and care products; especially suitable for infant washing and care products and washing and care products for AD patients.

[0051] As a preferred embodiment of the application described in this invention, the personal care product includes any one of shower gel, 2-in-1 shampoo and body wash, shampoo, and facial cleanser.

[0052] In a third aspect, the present invention provides a shower gel comprising the mild cleansing composition described herein.

[0053] As a preferred embodiment of the shower gel of the present invention, the shower gel comprises the following components by weight percentage: 25-50% mild cleansing composition, 0.5-2% emollient, 0.3-1.5% preservative, 0.05-2% thickener, 0.05-0.6% pH adjuster, 0.01-0.05% chelating agent, and the balance being water.

[0054] Preferably, the fat-adhesive includes at least one of glyceryl oleate and PEG-7 glyceryl cocoate.

[0055] Preferably, the preservative includes at least one of phenoxyethanol and sodium benzoate.

[0056] Preferably, the thickener includes at least one of PEG-150 distearate, PEG-120 methyl glucoside, hydroxypropyl methylcellulose, sodium chloride, and carbomer.

[0057] Preferably, the pH adjuster includes at least one of citric acid and lactic acid.

[0058] Preferably, the chelating agent includes at least one of disodium EDTA and tetrasodium EDTA.

[0059] This invention has found that by adding a mild cleansing composition within the above-mentioned mass percentage range to a shower gel, it is possible to achieve both good cleansing ability and skin protection; specifically, it can avoid damaging the skin barrier and leave low residue, and can effectively increase the skin's moisture content and improve skin barrier function after use.

[0060] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0061] This invention combines sodium lauroyl glutamate, decyl glucoside, Tween-28, cocamidopropyl betaine, plant saponins, and ectoine, and specifies that the plant saponins include camellia seed extract, as well as at least one of Acanthopanax senticosus extract, Sapindus mukorossi extract, and Gynostemma pentaphyllum extract. The synergistic effect of the above components can effectively improve the overall performance of the mild cleansing composition. When applied in the preparation of shower gel, the resulting shower gel has good mildness, low residue after use, can effectively increase the skin's moisture content, relieve post-bath skin dryness, and improve the skin's barrier function. Attached Figure Description

[0062] Figure 1 The image shows the results of the chicken embryo chorioallantoic membrane test in Example 1.

[0063] Figure 2 The graph shows the results of the chicken embryo chorioallantoic membrane test in Example 8, which is a comparison of the effects in Example 1.

[0064] Figure 3 The image shows the results of the red blood cell hemolysis test in Example 1.

[0065] Figure 4 The graph shows the results of the red blood cell hemolysis test in Example 8, which is used to compare the effects of Example 1. Detailed Implementation

[0066] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0067] Unless otherwise specified, the reagents, methods and equipment used in this invention are all conventional reagents, methods and equipment in the field; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch.

[0068] Eleutherococcus senticosus extract: purchased from Xi'an Xiaocao Botanical Technology Co., Ltd.;

[0069] Sapindus mukorossi extract: purchased from Guangdong Jingcui Biotechnology Co., Ltd.;

[0070] Gynostemma pentaphyllum extract: purchased from Jinhui (Guangzhou) Technology Co., Ltd.;

[0071] Camellia seed extract 1: Homemade, the preparation method includes the following steps:

[0072] (1) After washing and drying the camellia seed raw material, crush it and then pass it through a 40-mesh sieve. Collect the sieve material and mix it with deionized water at a mass ratio of 1:15. Then adjust the pH of the system to 5 with a citric acid / sodium citrate buffer system. Then add a compound enzyme preparation (a mixture of cellulase and papain at a mass ratio of 2:1, with the amount of compound enzyme preparation added being 3% based on the mass of the sieve material) and enzymatically hydrolyze it at 50°C for 120 min. After the enzymatic hydrolysis is completed, heat it to 90°C and keep it for 30 min to inactivate the enzyme and obtain the enzymatic hydrolysate.

[0073] (2) Add deionized water (the volume ratio of deionized water to enzymatic hydrolysate is 0.8:1) to the enzymatic hydrolysate and then extract it by ultrasonication at a frequency of 30kHz and a temperature of 40℃ for 50min. Then filter, collect the filtrate, concentrate and dry it to obtain camellia seed extract 1.

[0074] Camellia seed extract 2: prepared in-house. The difference between the preparation method and that of camellia seed extract 1 is that the mass ratio of cellulase and papain is 1:2.

[0075] Camellia seed extract 3: Homemade, the preparation method differs from that of camellia seed extract 1 in that cellulase is used instead of compound enzyme preparation.

[0076] Camellia seed extract 4: Homemade, the preparation method differs from that of camellia seed extract 1 in that papain is used instead of the compound enzyme preparation.

[0077] Plant saponin 1: A mixture formed by camellia seed extract 1, Acanthopanax senticosus extract and Gynostemma pentaphyllum extract in a mass ratio of 1:0.5:0.4;

[0078] Plant saponins 2: A mixture formed by camellia seed extract 1, Acanthopanax senticosus extract and Gynostemma pentaphyllum extract in a mass ratio of 0.5:1:0.4;

[0079] A mixture of plant saponins 3, camellia seed extract 1, and soapberry extract in a mass ratio of 1:0.9;

[0080] Plant saponins 4: a mixture of camellia seed extract 1 and gynostemma pentaphyllum extract in a mass ratio of 1:0.9;

[0081] Plant saponin 5: The difference from plant saponin 1 is that camellia seed extract 2 is used instead of camellia seed extract 1;

[0082] Plant saponin 6: The difference from plant saponin 1 is that camellia seed extract 3 is used instead of camellia seed extract 1;

[0083] Plant saponin 7: The difference from plant saponin 1 is that camellia seed extract 4 is used instead of camellia seed extract 1;

[0084] Plant saponins 8: a mixture of Sapindus mukorossi extract, Acanthopanax senticosus extract and Gynostemma pentaphyllum extract in a mass ratio of 1:0.5:0.4.

[0085] Examples 1-7 and Comparative Examples 1-4

[0086] The present invention provides a mild cleaning composition in the embodiments and comparative examples, wherein the components (parts by mass) of the mild cleaning composition are shown in Table 1; wherein W1 represents the mass percentage of ectoine based on the total mass of the mild cleaning composition, W2 represents the mass percentage of cocamidopropyl betaine based on the total mass of the mild cleaning composition, and W3 is the ratio of the sum of the mass of plant saponins and decyl glucoside to the sum of the mass of sodium lauroyl glutamate and Tween-28.

[0087] Table 1

[0088]

[0089] The mild cleaning composition provided in Example 1 is prepared by mixing the components evenly to obtain the mild cleaning composition.

[0090] The preparation methods of the mild cleaning compositions provided in Examples 2-7 and Comparative Examples 1-4 are consistent with those in Example 1; if the relevant components are not available, they can be omitted.

[0091] Example 8

[0092] This invention provides a mild cleaning composition, the only difference between the mild cleaning composition and Example 1 is that plant saponin 2 is used instead of plant saponin 1.

[0093] Example 9

[0094] This invention provides a mild cleaning composition, the only difference between the mild cleaning composition and Example 1 is that plant saponin 3 is used instead of plant saponin 1.

[0095] Example 10

[0096] This invention provides a mild cleaning composition, the only difference between the mild cleaning composition and Example 1 is that plant saponin 4 is used instead of plant saponin 1.

[0097] Example 11

[0098] This invention provides a mild cleaning composition, the only difference between the mild cleaning composition and Example 1 is that plant saponin 5 is used instead of plant saponin 1.

[0099] Example 12

[0100] This invention provides a mild cleaning composition, the only difference between the mild cleaning composition and Example 1 is that plant saponin 6 is used instead of plant saponin 1.

[0101] Example 13

[0102] This invention provides a mild cleaning composition, the only difference between the mild cleaning composition and Example 1 is that plant saponin 7 is used instead of plant saponin 1.

[0103] Comparative Example 5

[0104] The present invention provides a mild cleaning composition in a comparative example, the only difference between the mild cleaning composition and Example 1 being the use of plant saponin 8 instead of plant saponin 1.

[0105] Comparative Example 6

[0106] The present invention provides a mild cleaning composition in a comparative example, the only difference between the mild cleaning composition and Example 1 being the use of disodium cocoyl glutamate instead of sodium lauroyl glutamate.

[0107] Comparative Example 7

[0108] The present invention provides a mild cleaning composition in a comparative example, the only difference between the mild cleaning composition and Example 1 being the use of cocoyl glucoside instead of decyl glucoside.

[0109] Comparative Example 8

[0110] The present invention provides a mild cleaning composition in a comparative example, the only difference between the mild cleaning composition and Example 1 being the use of Tween-20 instead of Tween-28.

[0111] Comparative Example 9

[0112] The present invention provides a mild cleaning composition in a comparative example, the only difference between the mild cleaning composition and Example 1 being the use of trehalose instead of ectoine.

[0113] Comparative Example 10

[0114] The present invention provides a mild cleaning composition in a comparative example, the only difference between the mild cleaning composition and Example 1 being that lauryl hydroxysulfonate betaine is used instead of cocamidopropyl betaine.

[0115] Application Example 1-15, Comparison with Application Example 1-10 and Blank Application Example

[0116] The present invention provides a shower gel in application examples, comparative application examples, and blank application examples. The components (mass percentage) of the shower gel are shown in Table 2. In application examples 1-13, the mild cleansing compositions used are the mild cleansing compositions prepared in examples 1-13, for example, application example 1 uses the mild cleansing composition from example 1, application example 2 uses the mild cleansing composition from example 2, and so on. In comparative application examples 1-10, the mild cleansing compositions used are the mild cleansing compositions prepared in comparative examples 1-10. In application examples 14-15, the mild cleansing compositions used are the mild cleansing compositions prepared in example 1.

[0117] Table 2

[0118]

[0119] The method for preparing the shower gel provided in Example 1 includes the following steps:

[0120] (1) Add water and chelating agent to the main pot, heat to 70-85℃ with stirring until completely dissolved.

[0121] (2) Add sodium lauroyl glutamate, decyl glucoside, Tween-28, cocamidopropyl betaine and thickener to the main pot in sequence and stir until uniform.

[0122] (3) Cool down to 40-45℃, add the remaining material, and adjust the pH to 5.0-5.8. After stirring evenly, take a sample for testing and if it passes the test, the product can be discharged to obtain the shower gel.

[0123] The preparation methods of the shower gels provided in Application Examples 2-15, Comparative Application Examples 1-10, and Blank Application Examples are consistent with those in Application Example 1; if the relevant components are not available, they can be omitted.

[0124] Example 1

[0125] The effects of this invention are investigated in application examples, comparative application examples, and blank application examples to explore the residue and mildness of the shower gels prepared after use.

[0126] 1. Test for residue after use

[0127] The skin residue after using the shower gel was characterized by the indigo carmine staining method. Specifically, shower gels prepared for the application example and the control application example were taken and stained with the solution for sensitive skin (TEWL ≥ 20 g / hm). 2Three subjects per group were used as subjects for residue testing. A 4cm x 4cm area on the inner forearm of each subject was moistened with deionized water, treated with 2mL of shower gel for 2 minutes, then rinsed with deionized water and dried with a clean paper towel. The initial Lab value of the skin was measured using a colorimeter, and three parallel data points were obtained, with the average value calculated. 2mL of a 1% indigo carmine dye solution was applied for 1 minute. The dye was removed with deionized water, the area was rinsed, and dried with a clean paper towel. The Lab value of the skin was measured using a colorimeter, and three parallel data points were obtained, with the average value calculated. The value indicates the total color difference; the smaller the value, the less staining, meaning the most shower gel residue remains on the skin surface. Subjects 1-3 used sample 1 (Application Example 1) on the inner left forearm and sample 1 (Control Application Example 1) on the right, and so on. Subjects 28-30 used sample 10 (Application Example 10) on the inner left forearm and sample 10 (Control Application Example 10) on the right. Subjects 31-33 used sample 11 (Application Example 11) on the inner left forearm and a blank application example sample on the right. Subjects 34-36 used sample 12 (Application Example 12) on the inner left forearm and sample 13 (Application Example 13) on the right. Subjects 37-39 used sample 14 (Application Example 14) on the inner left forearm and sample 15 (Application Example 15) on the right. The results are shown in Table 4.

[0128] The calculation formula is as follows:

[0129] 2. Mildness test

[0130] The tests used included the chicken embryo chorioallantoic membrane test, the red blood cell hemolysis test, and the zein test.

[0131] 1) Chicken embryo testing includes the following steps: The chicken embryo chorioallantoic membrane test is performed, including the following steps: SPF-grade chicken embryos are incubated for 9 days in an incubator at 37.5℃±0.5℃, humidity 70%±5%, and a rotation frequency of 5 times / h. After incubation, the air cell location is marked, the eggshell is removed, and the egg membrane is moistened with 1mL of physiological saline and then removed, taking care to avoid damaging the vascular membrane. A Teflon ring is placed in the yolk position of the chicken embryo. The chicken embryo is placed under a stereomicroscope, and a photograph is recorded before sample loading. 40μL of shower gel is added to the Teflon ring, and a photograph is recorded at 30 seconds. Six chicken embryos are prepared in parallel for each sample.

[0132] Results Evaluation: Sample irritation was scored based on the presence of hemorrhage, coagulation, and vascular dissolution. No bleeding, mild bleeding, moderate bleeding, and severe bleeding were scored as 0, 1, 2, and 3 points respectively; no coagulation, mild coagulation, moderate coagulation, and severe coagulation were scored as 0, 1, 2, and 3 points respectively; and no vascular dissolution, mild vascular dissolution, moderate vascular dissolution, and severe vascular dissolution were scored as 0, 1, 2, and 3 points respectively. The endpoint score (ES) was calculated as: ES = (Sum of the observed degrees of hemorrhage, coagulation, and vascular dissolution in 6 chicken embryos) / 3. The scoring table is shown in Table 3, and the results are shown in Table 4.

[0133] Table 3

[0134] Finish line score Irritant Classification ES≤12 Non-irritating / mildly irritating 12<ES<16 moderate irritation ES≥16 Severe irritation

[0135] 2) The red blood cell hemolysis test includes the following steps: Prepare a 50,000 mg / L stock solution of the shower gel sample with PBS solution and dissolve it completely. Serially dilute the solution to obtain test solutions with mass / volume concentrations of 1000 mg / L, 900 mg / L, 800 mg / L, 700 mg / L, 600 mg / L, 500 mg / L, 400 mg / L, 300 mg / L, 200 mg / L, 100 mg / L, and 50 mg / L. Add 1.5 mL of the test solution to 0.5 mL of 2% sheep red blood cell suspension, incubate in a 32℃ water bath for 30 min, centrifuge at 13000 r / min for 3 min, and collect 300 μL of the supernatant. Use PBS as a blank control, 1000 mg / L sodium dodecyl sulfate (SDS) solution as a positive control, and purified water as a complete hemolysis control. Measure the absorbance at 560 nm wavelength. Each sample is tested in triplicate. Results calculation: Hemolysis rate % (HC%) = A(560) test solution / A(560) purified water × 100%. By comparing the sample concentration (HC50) that causes hemolysis of 50% of red blood cells, the irritant effect of the test sample can be compared. The results are shown in Table 4.

[0136] 3) The zein test includes the following steps: Prepare a 10% aqueous solution of the shower gel sample, add a certain amount of Zein until it no longer dissolves, stir at 330 rpm for 1 hour, let stand, and take 20 μL of the supernatant to dilute to 200 μL to obtain the test solution; dilute the 20 mg / mL protein standard (bovine serum albumin BSA) with purified water to 1.5 mg / mL, 1 mg / mL, 0.75 mg / mL, 0.5 mg / mL, 0.25 mg / mL, 0.125 mg / mL, and 0 mg / mL to plot a standard curve. Take 10 μL each of the protein standard and the test solution into a 96-well plate, in triplicate, add 300 μL of Coomassie Brilliant Blue G250 staining solution (detergent compatible), react at room temperature for 20 min, and measure the absorbance at A595 nm. Calculate the protein concentration in each test sample using the linear equation plotted with the protein standard. The greater the degree of solubility, the higher the interaction between the sample and Zein, and the stronger the irritation.

[0137] The results are shown in Table 4.

[0138] Table 4

[0139]

[0140]

[0141] As can be seen from Table 4, the shower gel provided by this invention has excellent gentleness and leaves little residue after use; specifically, The concentrations of chicken embryos should be above 10.18, the chicken embryo test score should be below 4.33, the HC50 of the hemolytic red blood cell test should be above 942 mg / L, and the protein concentration of the zein test should be below 1.86 mg / mL.

[0142] As can be seen from Application Examples 1-7 and Comparative Application Examples 1-4, the mass fraction, mass percentage, and mass ratio of the components in the mild cleaning composition provided by the present invention have a significant impact on the overall performance of the prepared shower gel. When any one of the components in the mild cleaning composition of Comparative Application Examples 1-4 is missing, the residual amount of the shower gel after use is significantly increased, and the irritation is relatively high. When the mass fraction of the components in the mild cleaning composition is further selected within the preferred range of the present invention, the overall effect of the obtained shower gel is even better. Specifically, the shower gel before and after use... The concentrations of the protein in the chicken embryo test should be above 10.86, the chicken embryo test score should be below 2.67, the HC50 in the hemolysis test should be above 976 mg / L, and the protein concentration in the zein test should be below 1.36 mg / mL.

[0143] As can be seen from Application Example 1, Application Examples 8-13 and Comparative Application Example 5, the selection of plant saponins affects the overall performance of the prepared shower gel. When the plant saponins selected in Comparative Application Example 5 are not the substances in this invention, the residual amount of the shower gel after use is significantly increased, and the irritation is relatively high.

[0144] As can be seen from Application Example 1 and Comparative Application Examples 6-10, the effects of the present invention cannot be achieved when similar substances are used to replace the components in the present invention.

[0145] The results of the chicken embryo chorioallantoic membrane test in Application Example 1 and Comparative Application Example 8 are shown in the figures below. Figure 1 , Figure 2 As shown; the results of the red blood cell hemolysis test in Application Example 1 and Comparative Application Example 8 are shown in the following figures. Figure 3 , Figure 4 As shown.

[0146] Example 2

[0147] The effects of the shower gels prepared by the present invention in the experimental application examples, comparative application examples, and blank application examples on skin moisture content and skin barrier are investigated.

[0148] Shower gels prepared in the application example and the control application example were taken separately and tested on sensitive skin (TEWL≥20g / hm). 2 Three subjects per group underwent tests to measure stratum corneum moisture content and transdermal water loss. Subjects 1-3 used the sample from Application Example 1 on the inner left forearm and the control sample from Application Example 1 on the right, and so on. Subjects 28-30 used the sample from Application Example 10 on the inner left forearm and the control sample from Application Example 10 on the right. Subjects 31-33 used the sample from Application Example 11 on the inner left forearm and the blank sample on the right. Subjects 34-36 used the sample from Application Example 12 on the inner left forearm and the sample from Application Example 13 on the right. Subjects 37-39 used the sample from Application Example 14 on the inner left forearm and the sample from Application Example 15 on the right. On the day of the test, no product was applied to the test area. After washing, subjects sat quietly for 20 minutes in a constant temperature and humidity room at 21±1℃ and 50±10%. The transdermal water loss probe was used. TM Hex (Courage+Khazaka) and Moisture Measurement Probe The CM825 measured the initial TEWL and MMV values ​​for each volunteer and averaged the three results. Subjects used the shower gel sample twice daily (morning and evening) for 28 consecutive days. TEWL and MMV values ​​in the tested areas were then measured again after use; the rate of change was subsequently calculated.

[0149] TEWL value change rate (%) = (initial TEWL value - TEWL value after use) / initial TEWL value × 100%;

[0150] MMV improvement rate (%) = (MMV value after use - initial MMV value) / initial MMV value × 100%;

[0151] A larger and positive TEWL value change rate indicates that the sample is friendly to the skin barrier and has the ability to improve the skin barrier. A larger MMV value improvement rate indicates that the shower gel has a better long-lasting moisturizing effect.

[0152] The results are shown in Table 5.

[0153] Table 5

[0154]

[0155] As can be seen from Table 5, the shower gel prepared using the mild cleansing composition of the present invention has excellent ability to increase skin moisture content and improve skin barrier function; specifically, the improvement rate of MMV value before and after use of the shower gel is more than 16.09%, and the change rate of TEWL value, which reflects skin barrier function, is more than 10.58%.

[0156] As can be seen from Application Examples 1-7 and Comparative Application Examples 1-4, the mass fraction of the components, the mass percentage of the components, and the mass ratio between the components in the mild cleansing composition provided by the present invention have a significant impact on the overall performance of the prepared shower gel. When any one of the components in the mild cleansing composition of Comparative Application Examples 1-4 is missing, the resulting shower gel has a poor moisturizing effect and poses a risk of skin barrier damage. When the mass fraction of the components in the mild cleansing composition is further selected within the preferred range of the present invention, the overall effect of the resulting shower gel is even better. Specifically, the improvement rate of the MMV value before and after use of the resulting shower gel is above 18.16%, and the change rate of the TEWL value, which reflects the skin barrier capacity, is above 13.02%.

[0157] As can be seen from Application Example 1, Application Examples 8-13 and Comparative Application Example 5, the selection of plant saponins affects the overall performance of the prepared shower gel. When the plant saponins selected in Comparative Application Example 5 are not substances in this invention, the shower gel basically does not show any skin care effect.

[0158] As can be seen from Application Example 1 and Comparative Application Examples 6-10, the effects of the present invention cannot be achieved when similar substances are used to replace the components in the present invention.

[0159] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention and not to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A mild cleaning composition, characterized in that, The mild cleaning composition comprises the following components in parts by weight: 0.5-8 parts sodium lauroyl glutamate, 1-8 parts decyl glucoside, 0.5-8 parts Tween-28, 0.5-2.5 parts cocamidopropyl betaine, 0.05-5 parts plant saponins, and 0.05-2 parts ectoine; The plant saponins include camellia seed extract, Acanthopanax senticosus extract, and Gynostemma pentaphyllum extract, wherein the mass ratio of the camellia seed extract, Acanthopanax senticosus extract, and Gynostemma pentaphyllum extract is 1:(0.2-0.8):(0.1-0.6). The ectoine mass percentage is ≥0.29% based on the total mass of the mild cleaning composition; The mass percentage of cocamidopropyl betaine is ≥2.62% based on the total mass of the mild cleaning composition. The ratio of the sum of the masses of the plant saponins and decyl glucoside to the sum of the masses of sodium lauroyl glutamate and Tween-28 is ≥0.4; The preparation method of the camellia seed extract includes the following steps: (1) Add the dried and pulverized camellia seeds to deionized water, and then add a compound enzyme preparation for enzymatic hydrolysis and enzyme inactivation; (2) Add deionized water to the enzyme hydrolysate after enzyme inactivation treatment and extract by ultrasonication. After extraction, separate the solid and liquid, collect the liquid and concentrate and dry it to obtain camellia seed extract. In step (1), the compound enzyme preparation is cellulase and papain, and the mass ratio of cellulase to papain is 2:(0.5-1.5).

2. The mild cleaning composition according to claim 1, characterized in that, The mild cleaning composition comprises the following components in parts by weight: 5-6 parts sodium lauroyl glutamate, 3-5 parts decyl glucoside, 3-6 parts Tween-28, 1-2 parts cocamidopropyl betaine, 1.5-3 parts plant saponins, and 1-1.5 parts ectoine.

3. The mild cleaning composition according to claim 1, characterized in that, In step (1), the mass percentage of the compound enzyme preparation is 2-4% based on the mass of camellia seeds; And / or, in step (1), the enzymatic hydrolysis temperature is 50±5℃, the enzymatic hydrolysis time is 90-150min, and the enzymatic hydrolysis pH value is 4.5-5.5; And / or, in step (1), the mass ratio of camellia seeds to deionized water is 1:(10-20); And / or, in step (2), the ultrasonic frequency of ultrasonic extraction is 20-40kHz and the ultrasonic time is 40-60min.

4. The use of the mild cleaning composition according to any one of claims 1-3 in the preparation of personal care products.

5. A shower gel, characterized in that, The shower gel comprises the mild cleansing composition as described in any one of claims 1-3.

6. The shower gel according to claim 5, characterized in that, The shower gel comprises the following components by weight percentage: 25-50% of the mild cleansing composition as described in any one of claims 1-3, 0.5-2% of a fatliquoring agent, 0.3-1.5% of a preservative, 0.05-2% of a thickener, 0.05-0.6% of a pH adjuster, 0.01-0.05% of a chelating agent, and the balance being water.

Images