A full-bio-based intimate care gel and method of making

Abstract

The present application belongs to the field of daily chemical products, and particularly relates to a full-biological private care gel composition and a preparation method thereof. The composition comprises a biosugar lipid active substance, a lipopeptide and a plant saponin. The formula of the present application is experimentally proved to have the functions of inhibiting Staphylococcus aureus, Escherichia coli and Aspergillus niger, and removing the biofilm formed by Staphylococcus aureus and Candida albicans.

Description

Technical Field

[0001] This invention belongs to the field of daily chemical products, specifically relating to a fully bio-based feminine hygiene gel composition, and more specifically to a method for preparing a fully bio-based feminine hygiene gel. Background Technology

[0002] With increasing health awareness among women, growing demand for personal care products, and the trend of consumption upgrading, the feminine hygiene industry has experienced rapid growth in the Chinese market in recent years. Feminine hygiene products cover multiple areas, from daily care to professional treatment, including feminine washes, wipes, gels, and feminine cosmetics. This has led to a continuous increase in demand for natural, healthy, additive-free, and non-irritating products, prompting the industry to place greater emphasis on natural ingredients and healthy formulas. For example, the market share of feminine hygiene products containing plant extracts and green fermentation active products is gradually expanding. The industry is also developing feminine hygiene products containing probiotics, plant extracts, and bioactive ingredients that regulate the vaginal microecology, have anti-inflammatory, soothing, and nourishing functions. Furthermore, the growing demand for personalized products is driving the industry towards customization. In the near future, some companies will offer more personalized, refined, and differentiated feminine hygiene products tailored to different ages, skin types, physiological stages, lifestyles, and health conditions, such as products for sensitive skin, pregnancy, and menopause. Summary of the Invention

[0003] Based on the above background, this invention, through research, identifies a composition containing bioactive glycolipids, lipopeptides, and plant saponins, and through formulation optimization, achieves a bio-based component in the formula that can regulate the vaginal microecological balance, promote the growth and reproduction of beneficial bacteria, and inhibit the growth of harmful bacteria, thereby achieving a synergistic antibacterial effect, and ultimately completing this invention.

[0004] In a first aspect, the present invention provides a fully bio-based feminine care gel composition comprising bioactive glycolipids, lipopeptides and plant saponins; wherein the two or three are combined in a mass ratio of 0.5-2:0.02-0.05:0.02-0.05.

[0005] According to a preferred embodiment of the present invention, the bio-glycolipid surfactant is sophorolipid and / or rhamnolipid.

[0006] According to a preferred embodiment of the present invention, the sophorolipid is obtained by inoculating *Candida aegyptiacus* onto glucose and vegetable oil as substrates, fermenting under aseptic conditions, and then separating and purifying the resulting solution. *Candida aegyptiacus* is also known as male *Candida aegyptiacus* and *Candida globulata*.

[0007] According to a preferred embodiment of the present invention, the rhamnosyl ester is prepared by inoculating a Pseudomonas microorganism into a carbon source and vegetable oil, fermenting under aseptic conditions, and then separating and purifying the product. The Pseudomonas microorganism may be Pseudomonas aeruginosa or Pseudomonas putida; the carbon source is at least one of glucose, glycerol, and citric acid.

[0008] According to a preferred embodiment of the present invention, the glycolipid is sophorolipid or rhamnolipid, for example, selected from mature commercial products such as sophorolipid, rhamnolipid, or a mixture of sophorolipid and rhamnolipid produced and sold by Shanghai Boku Biotechnology Co., Ltd.

[0009] According to a preferred embodiment of the present invention, the lipopeptide is a secondary metabolite (usually a mixture) produced during microbial fermentation. It is typically a type of biosurfactant composed of β-amino or β-hydroxy fatty acids (lipophilic groups) and peptide chains or peptide rings (hydrophilic groups). Substance lipopeptide sodium is a lipopeptide biosurfactant, also known as a surfactant, produced by Bacillus subtilis strains through fermentation. Its hydrophilic group consists of a cyclic peptide. This cyclic peptide, composed of seven amino acids, possesses abundant hydrogen bond acceptors and donors, making it a bio-based anionic surfactant. Lipopeptides offer advantages such as being green and safe, and are currently a hot topic in surfactant research.

[0010] According to a preferred embodiment of the present invention, the lipopeptide is sodium subtilis lipopeptide, for example, selected from the commercially available sodium subtilis lipopeptide produced and sold by Shanghai Boku Biotechnology Co., Ltd.

[0011] According to a preferred embodiment of the present invention, the plant saponin is a natural nonionic surfactant composed of saponins, sugars, uronic acids, or other organic acids. It possesses strong biological activity and is widely used in cosmetics, detergents, and pharmaceuticals. Tea seed meal, a byproduct of oil extraction from tea seeds, is the primary source of plant saponins. Currently, tea seed meal is mainly used for reforestation and is not comprehensively recycled. Therefore, fully utilizing tea seed meal saponins and efficiently utilizing this waste can not only avoid resource waste and protect the environment but also reduce the cost of daily chemical products.

[0012] According to a preferred embodiment of the present invention, the plant saponin is tea saponin.

[0013] Secondly, the present invention also provides a fully bio-based feminine hygiene gel product comprising the above-described composition.

[0014] Preferably, the all-bio-based feminine hygiene gel product further includes one or more of the following: thickener, moisturizer, preservative, conditioning agent, buffer, and fragrance.

[0015] The thickener is hydroxyethyl cellulose or xanthan gum; the moisturizer is glycerin and sorbitol; the preservative is methylparaben or polylysine; the conditioning agent is plant hydrosol and nicotinamide; and the buffer is citric acid.

[0016] Hydroxyethyl cellulose is a white, free-flowing, powdery, nonionic, water-soluble polymer. Its nonionic nature allows it to coexist with a wide range of other water-soluble polymers and surfactants, making it an excellent colloidal thickener for solutions containing high concentrations of dielectrics. Sorbitol enhances the spreadability and lubricity of emulsifiers, improves moisturizing properties, and has some hygroscopicity, making it suitable for long-term storage. Methylparaben is a widely used preservative in the daily chemical industry. It effectively inhibits the growth of bacteria, molds, and yeasts by disrupting microbial cell membranes and inducing intracellular protein denaturation. When used alone, its content should not exceed 0.4%.

[0017] According to a preferred embodiment of the present invention, the all-bio-based feminine hygiene gel product, by weight percentage, comprises: 10.0-15.0% glycerin, 4.0-6.0% sorbitol, 0.8-1.0% hydroxyethyl cellulose, 0.10-0.15% methylparaben, and 0.50-1.20% of the composition; the balance being water.

[0018] Its second component consists of: 6.0-8.0% glycerol, 0.50-0.80% xanthan gum, 0.10-0.20% polylysine, 0.50-1.20% of the composition, 0.20%-0.30% nicotinamide, and 0.03-0.05% citric acid; the balance is plant hydrosol.

[0019] Thirdly, this invention provides a method for preparing the aforementioned all-bio-based feminine hygiene gel product, comprising the following steps:

[0020] Mix the humectant and preservative and heat to 45-55℃;

[0021] Add thickener until the powder is completely dispersed and free of lumps;

[0022] Add the humectant, surfactant, and active ingredient while maintaining a temperature of 40-50℃, and continue stirring for 30-90 minutes. After stirring, keep the gel at a high temperature of 85-90℃ for 5-15 minutes.

[0023] Specifically, the process includes the following steps: glycerol and methylparaben are mixed and heated to 50°C; hydroxyethyl cellulose is added until the powder is completely dispersed and free of lumps, and the mixture is kept at 45°C. Then, sorbitol, lactone-type sophorolipid Ecolife SL-02, tea saponin, and water are added, and the mixture is stirred for 60 minutes. The resulting gel is then kept at 85-90°C for 5-15 minutes, hot-filled into bottles, and the pH is measured to be 5.50.

[0024] Specifically, the process includes the following steps: glycerol and methylparaben are mixed and heated to 50°C; hydroxyethyl cellulose is added until the powder is completely dispersed and free of lumps, and the mixture is kept at 45°C. Sorbitol, sophorolipid Ecolife SL-03, tea saponin, and water are then added, and the mixture is stirred for 60 minutes. The resulting gel is then kept at 85-90°C for 5-15 minutes, hot-filled into bottles, and the pH is measured to be 5.65.

[0025] In another specific embodiment, the following steps are included: first, glycerol is used to wet and disperse xanthan gum, then plant hydrosol, lactone-type sophorolipid, acid-type sophorolipid, sodium subtilis lipopeptide, tea saponin, nicotinamide, and polylysine are added in sequence, and finally citric acid is added to adjust the pH. The resulting gel is kept at a high temperature of 85-90℃ for 5-15 minutes, hot-filled into bottles, and the pH is measured to be 6.20.

[0026] Beneficial effects

[0027] Beneficial effects of this invention: This invention discloses a fully bio-based vaginal gel that uses bio-glycolipids, lipopeptides, and plant saponins to inhibit bacterial growth in the vagina, thereby preventing and improving some gynecological problems. Bio-glycolipids, lipopeptides, and plant saponins are gentler and more environmentally friendly than traditional surfactants. They are not only stable in efficacy but also inhibit pathogens such as Staphylococcus aureus, Candida albicans, and Escherichia coli, reducing vaginal discomfort caused by these pathogens, such as itching, burning, and odor. Furthermore, they maintain and improve the health of the vaginal mucosa, regulate the vaginal pH environment, help maintain a normal vaginal microecological environment, reduce pathogen growth, and prevent infection and inflammation.

[0028] Experiments have shown that this invention can play a role in inhibiting Staphylococcus aureus, Escherichia coli, and Aspergillus niger, as well as in removing biofilms formed by Staphylococcus aureus and Candida albicans. Attached Figure Description

[0029] Figure 1 The results of a plate experiment show the inhibitory effect of the feminine hygiene gel in Example 1 on Staphylococcus aureus (ATCC 27217).

[0030] Figure 2This is a microscopic observation of the effect of the feminine hygiene gel in Example 1 on the removal of Candida albicans biofilm;

[0031] Figure 3 The results of a plate experiment show the inhibitory effect of the feminine hygiene gel on Aspergillus niger (ATCC 16404) in Example 1.

[0032] Figure 4 Example 2: Plate experiment results of the inhibitory effect of feminine hygiene gel on Aspergillus niger (ATCC 16404);

[0033] Figure 5 Example 3: Plate experiment results of the inhibitory effect of feminine hygiene gel on Staphylococcus aureus (ATCC 27217);

[0034] Figure 6 Example 3: Microscopic observation of the effect of feminine hygiene gel on the removal of Candida albicans biofilm;

[0035] Figure 7 Example 3: Plate experiment results of the inhibitory effect of feminine care gel on Aspergillus niger (ATCC 16404). Detailed Implementation

[0036] To make the above-mentioned objectives, features and advantages of the present invention more readily understood, the specific embodiments of the present invention will be described in detail below with reference to specific implementations.

[0037] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0038] Glycolipids are a new generation of green fermented glycolipid biosurfactants. They are mild and non-irritating, have good surface activity, are environmentally friendly, and possess antibacterial and anti-inflammatory properties. Adding a certain amount of glycolipids can help improve the overall mildness of the surfactant and the skin barrier function. They have an amphiphilic molecular structure, containing a hydrophilic glycosyl head and a hydrophobic fatty acid tail, with a critical micelle concentration (CMC) as low as 20-50 mg / L. They are 100% biodegradable, with a natural degradation rate of >98% within 28 days (OECD 301D standard).

[0039] Lipopeptides represent the forefront of synthetic biology in the field of surfactants, possessing excellent antibacterial properties. Due to their unique cyclic peptide structure, hydrogen bonds can form between molecules when they form micelles in water, giving them a stronger binding capacity than other surfactants. Therefore, they can begin to form polymers with a very small number of molecules, resulting in a higher surface adsorption capacity of 8.472 μmol / m³. 2Its molecular cross-sectional area is even lower, at 0.196 nm. 2 The molecular cross-sectional area is related to the structure; the smaller the molecular cross-sectional area, the more tightly packed the molecules are arranged at the interface. It also possesses certain biological activities, such as antibacterial, anti-inflammatory, antioxidant, and anti-pollution properties.

[0040] Tea saponin, a plant saponin belonging to the saponin class, possesses the basic properties of saponins. The structure of tea saponin can be divided into hydrophilic and lipophilic groups. Key hydrophilic groups include strongly electronegative hydroxyl and carboxyl groups, mostly sugars and organic acids in tea saponin. These groups typically appear between sugar ligands or at the junctions of saponins and organic acid ligands in the tea saponin structure. Lipophilic groups are saponin ligands formed by nonpolar hydrocarbon ring chains, such as derivatives of β-amyrin. These groups exhibit hydrophobic properties in aqueous solution, forming the lipophilic main body of tea saponin. The hydrophilic and lipophilic groups, respectively, exhibit hydrophilic and lipophilic properties in solution. These two types of groups interact to give tea saponin its surface activity. In addition, it also possesses antibacterial, anti-inflammatory, anti-permeability, antioxidant, and hemolytic effects.

[0041] Mixing two or three of the above components creates a bio-based formula that can regulate the vaginal microecological balance, promote the growth and reproduction of beneficial bacteria, and inhibit the growth of harmful bacteria, thereby achieving a synergistic antibacterial effect.

[0042] The following describes the scheme with reference to embodiments of the present invention. The sophorolipids and rhamnolipids used in the embodiments are four types of glycolipids produced by Shanghai Bokoo Biotechnology Co., Ltd.: Ecolife SL-02, Ecolife SL-03, Ecolife RP-01, and Ecolife RS-20. Ecolife SL-02 is a lactone-type sophorolipid, Ecolife SL-03 is an acid-type sophorolipid, Ecolife RP-01 is a rhamnolipid, and Ecolife RS-20 is a mixture of acid-type sophorolipids and rhamnolipids. The subtilis lipopeptide sodium used in the embodiments is subtilis lipopeptide sodium produced by Shanghai Bokoo Biotechnology Co., Ltd., and its model is Ecolife SF100.

[0043] Example 1

[0044] Formulate a fully bio-based feminine care gel. The ingredients of the feminine care gel are shown in Table 1.

[0045] Table 1

[0046]

[0047] The above-mentioned feminine care gel is prepared as follows: glycerin and methylparaben are mixed and heated to 50°C; hydroxyethyl cellulose is added until the powder is completely dispersed and free of lumps, and the temperature is maintained at 45°C. Then, sorbitol, lactone-type sophorolipid Ecolife SL-02, tea saponin, and water are added, and stirring is continued for 60 minutes. The gel obtained after stirring is kept at a high temperature of 85-90°C for 5-15 minutes, hot-filled into bottles, and the pH is measured to be 5.50.

[0048] This embodiment tested the antibacterial activity of the all-biobased feminine hygiene gel against Staphylococcus aureus (ATCC 27217). Experimental methods: (1) Preparation of culture medium: Weigh LB broth according to experimental requirements, heat and stir to dissolve in distilled water, autoclave at 121℃ for 15 min, and set aside. Weigh LB nutrient agar medium according to experimental requirements, heat and stir to dissolve in distilled water, autoclave at 121℃ for 15 min, and set aside. After cooling the LB agar medium and pouring it into plates, invert it after solidification. (2) Staphylococcus aureus strain activation: Staphylococcus aureus glycerol tube strain was stored at -80℃, inoculated onto LB agar plates, and cultured at 37℃ for 12-24h until single colonies grew, and then used; (3) Staphylococcus aureus suspension preparation: single colonies that had been cultured were picked, inoculated into LB liquid medium, shaken and cultured at 220 rpm at 37℃ for 12-24h, and serially diluted Staphylococcus aureus (ATCC 27217) suspension (diluted as needed according to the bacterial concentration (bacterial content of 1×10¹⁰ cfu / mL)) for antibacterial testing, and kept for later use. (4) LB agar medium for feminine hygiene gel: after sterilization, LB agar medium was cooled to below 50 degrees Celsius, and glycolipid was added quantitatively under aseptic conditions to make the final concentration reach the required concentration (20%) for the test, shaken well, poured into plates and cooled, and kept for later use. (5) Antibacterial test: Take 100 µl of the diluted Staphylococcus aureus suspension in step (3) and inoculate it on gel LB agar medium plate and LB agar medium plate without added sugar lipids. Spread it evenly with a spreader. After incubating in a 37℃ incubator for 12-24h, count the number of colonies on the two plates.

[0049] Reference Figure 1 Example 1: Inhibitory effect of feminine hygiene gel on Staphylococcus aureus (ATCC 27217). 10% of Example 1 feminine hygiene gel completely inhibited Staphylococcus aureus (ATCC 27217).

[0050] Biofilms, as microbial secretions, are commonly found in production or water systems in the pharmaceutical, food, and personal care industries. Biofilms can significantly increase the resistance of microorganisms to harsh environments and disinfectants, and continuously attract planktonic microorganisms, leading to frequent exceedances of microbial levels in production or water systems even after cleaning and disinfection. Therefore, this study investigates the effect of feminine hygiene gels on Candida albicans biofilm formation. The key to controlling frequent biofilm or microbial exceedances lies in removing biofilms composed of extracellular polymers.

[0051] This embodiment tested the removal of Candida albicans biofilm by a fully bio-based feminine hygiene gel. Experimental methods: (1) Preparation of strains: Candida albicans strains frozen at -80℃ were inoculated on YPD agar plates and cultured at 29℃ for 24-48 hours. Single colonies were picked and inoculated into YPD liquid medium, shaken and cultured at 29℃ and 200 rpm for 18-24 hours for later use. (2) Observation of the effect of glycolipid feminine hygiene gel on Candida albicans biofilm under a microscope: 2 mL of Candida albicans bacterial suspension (1:100 dilution) was added to a 6-well plate, and a sterilized coverslip was placed in it. The plate was then placed at 29℃ and cultured statically for 48 hours to form a Candida albicans biofilm. After the Candida albicans biofilm was formed, the culture medium was carefully aspirated, and the plate was washed three times with sterile water. Then, 2 mL of glycolipid feminine hygiene gel was injected, shaken and cultured at 29℃ and 150 rpm for 5 min, 15 min, 30 min, 90 min, and 24 h. After the culture was completed, the gel and other bacteria were washed three times with sterile water. Then, the effects of the feminine hygiene gel on the Candida albicans biofilm were observed under a microscope using the 0.01% crystal violet staining method.

[0052] Reference Figure 2 Example 1: The effect of feminine care gel on Candida albicans biofilm removal. The feminine care gel in Example 1 has a very good effect on removing Candida albicans biofilm. After co-culturing for 5 minutes, the feminine care gel showed a very good removal effect.

[0053] This embodiment tested the inhibition of Aspergillus niger (ATCC 16404) by the all-bio-based feminine hygiene gel. Experimental methods: (1) Preparation of culture medium: commercially available potato dextrose agar (PDA) medium was dissolved in water as required and sterilized at 121℃ for 15 minutes. After the potato dextrose agar medium was cooled and poured into plates, it was inverted after solidification. (2) Activation of Aspergillus niger strain: Aspergillus niger glycerol tube strain was stored at -80℃, inoculated onto PDA agar plates, and cultured in a 29℃ incubator for 5-7 days until complete colonies grew. (3) Preparation of Aspergillus niger suspension: 10 mL of sterile water was added to the plate, and the spore surface was gently scraped with a sterile scraper and transferred to a sterile centrifuge tube. The plate was vortexed and shaken evenly, and allowed to stand for 5-10 minutes to allow the hyphae to settle before use. (4) Preparation of PDA agar plates with glycolipids: After sterilization, PDA agar medium was cooled to below 50 degrees Celsius. Cinnamon extract was added quantitatively under aseptic conditions to achieve the final concentration required for the experiment. The plates were shaken well and then cooled for later use. (5) Antibacterial test: An appropriate amount of sterile filter paper was placed in a sterile plate. The upper spore suspension from step (3) was placed in a sterile plate containing sterile filter paper and soaked for 2 hours. The filter paper was placed on the middle surface of PDA plates with different concentrations of glycolipids using sterile forceps. The control was Aspergillus niger filter paper placed on a plate without glycolipids. The plate was covered and placed in a 29°C incubator for 5-7 days. The diameter of inhibition (mm) was measured with a ruler to evaluate the antibacterial activity. The formula for calculating the antibacterial rate is as follows:

[0054] .

[0055] The results are as follows Figure 3 The plate suppression situation is shown in Figure 2, and the statistical data are shown in Table 2.

[0056] Table 2. Inhibition rate of feminine hygiene gel against Aspergillus niger (ATCC 16404) in Example 1

[0057]

[0058] Among them, the feminine hygiene gel of Example 1 showed an inhibitory effect on Aspergillus niger (ATCC 16404). When the concentration of the feminine hygiene gel of Example 1 was 8%, the antibacterial rate against Aspergillus niger reached 56%, which showed that the compound feminine hygiene gel of tea saponin and lactone-type sophorolipid Ecolife SL-02 had good antibacterial activity against Aspergillus niger.

[0059] Example 2

[0060] Formulate a fully bio-based feminine care gel. The ingredients of the feminine care gel are shown in Table 3.

[0061] Table 3

[0062]

[0063] The above-mentioned feminine care gel is prepared as follows: glycerin and methylparaben are mixed and heated to 50°C; hydroxyethyl cellulose is added until the powder is completely dispersed and free of lumps, and the temperature is maintained at 45°C. Then, sorbitol, sophorolipid Ecolife SL-03, tea saponin, and water are added, and stirring is continued for 60 minutes. The gel obtained after stirring is kept at a high temperature of 85-90°C for 5-15 minutes, hot-filled into bottles, and the pH is measured to be 5.65.

[0064] This example tested the inhibition of Aspergillus niger (ATCC 16404) by the all-biobased feminine hygiene gel. The experimental method was the same as that in Example 1.

[0065] The results are as follows Figure 4 The plate inhibition results are shown in Figure 2 and the inhibition rate statistics are shown in Table 4.

[0066] Table 4. Inhibition rate of feminine hygiene gel against Aspergillus niger (ATCC 16404) in Example 2

[0067]

[0068] In Example 2, when the concentration of the feminine care gel was 10%, the antibacterial rate against Aspergillus niger reached 56%, demonstrating that the compound feminine care gel of tea saponin and acidic sophorolipid Ecolife SL-03 has good antibacterial activity against Aspergillus niger.

[0069] Example 3

[0070] Formulate a fully bio-based feminine care gel. The ingredients of the feminine care gel are shown in Table 5.

[0071] Table 5

[0072]

[0073] The preparation method of the above-mentioned feminine hygiene gel is as follows: Prepare at room temperature by first wetting and dispersing xanthan gum with glycerin, then sequentially adding plant hydrosol, lactone-type sophorolipid, acid-type sophorolipid, sodium subtilisin, tea saponin, nicotinamide, and polylysine. Finally, add citric acid to adjust the pH. Incubate the resulting gel at 85-90℃ for 5-15 minutes, then hot-fill into bottles. The pH is measured to be 6.20. The plant hydrosols used in this formula can be Damask rose hydrosol, chamomile hydrosol, calendula hydrosol, frankincense hydrosol, etc.

[0074] This embodiment tested the antibacterial activity of the all-biobased feminine hygiene gel against Staphylococcus aureus, using the same experimental method as in Example 1. (Refer to...) Figure 5 The feminine hygiene gel of Example 3 showed an inhibitory effect on Staphylococcus aureus (ATCC 27217). 20% of the feminine hygiene gel of Example 3 completely inhibited Staphylococcus aureus.

[0075] This embodiment tested the removal of Candida albicans biofilm by the all-biobased feminine hygiene gel, using the same experimental method as in Example 1. (Refer to...) Figure 6 The feminine hygiene gel in Example 3 showed a good effect in removing Candida albicans biofilm. After 15 minutes of co-culturing, the feminine hygiene gel exhibited a very good removal effect.

[0076] This example tested the inhibitory effect of the all-biobased feminine hygiene gel on Aspergillus niger (ATCC 16404), using the same experimental method as in Example 1. The results are as follows: Figure 7 The plate inhibition results are shown in Figure 2 and the inhibition rate statistics are shown in Table 6.

[0077] Table 6. Inhibition rate of feminine hygiene gel against Aspergillus niger (ATCC 16404) in Example 3

[0078]

[0079] The results showed that when the concentration of the feminine care gel in Example 3 was 3%, the antibacterial rate against Aspergillus niger reached 99%, demonstrating very good antibacterial activity against Aspergillus niger.

[0080] Performance tests were conducted on the above embodiments: Low-temperature stability: Equal amounts of the feminine care gel from each embodiment were placed at -5℃ for 24 hours, and after returning to room temperature, the presence of precipitation and stratification was observed; High-temperature stability: Equal amounts of the feminine care gel from each embodiment were placed at 45℃ for 24 hours, and after returning to room temperature, the presence of precipitation and stratification was observed. The test results are shown in Table 7.

[0081] Table 7

[0082]

[0083] The above embodiments underwent soothing tests: common allergic symptoms such as redness, swelling, itching, and pain are mainly caused by histamine. There is a strong correlation between hyaluronidase activity inhibition and mast cell histamine release inhibition activity. Histamine-inhibiting active ingredients often also exhibit significant hyaluronidase inhibitory activity. Hyaluronidase is strongly correlated with inflammation and allergies. Some anti-allergy drugs strongly inhibit hyaluronidase activity; therefore, hyaluronidase activity inhibition is used as an indicator for studying anti-allergic effects and is used to explore in vitro anti-allergy active ingredients. Elson-Morgan method: Hyaluronic acid can be hydrolyzed under conditions with a certain level of hyaluronidase activity to produce β-N-acetylglucosamine. The latter can condense with acetylacetone under alkaline catalysis to form a chromogen. This chromogen condenses with Ehrlich reagent under acidic conditions, and the condensation product has maximum absorption at 530 nm. Therefore, by determining the content of β-N-acetylglucosamine by visible spectrophotometry, the degree of hyaluronic acid hydrolysis can be determined, thus indirectly judging hyaluronidase activity. The higher the content of this component, the higher the enzyme activity, and vice versa.

[0084] The test results are shown in Table 8. According to international standards, all three embodiments described above exhibit strong anti-allergic and anti-inflammatory effects.

[0085] Table 8

[0086]

[0087] As described above, this application may have various modifications and variations. Any modifications, equivalent substitutions, improvements, etc., made within the technical concept and principles of this application shall be included within the scope of protection of this application.

Claims

1. A fully bio-based feminine hygiene gel composition, characterized in that, It contains bioactive glycolipids, lipopeptides, and plant saponins, and the bioactive glycolipids, lipopeptides, and plant saponins are combined in a mass ratio of 0.5-2:0.02-0.05:0.02-0.

05.

2. The composition according to claim 1, characterized in that, The bio-glycolipid surfactant is sophorolipid or rhamnolipid or a mixture thereof; The sophorolipid is one or a mixture of lactone-type sophorolipid, acid-type sophorolipid, rhamnolipin, sophorolipid, and rhamnolipin; Preferably, the sophorolipid is a mixture of lactone-type sophorolipid and acid-type sophorolipid in a weight ratio of 2-4:2 (specifically, 3:2); More specifically, the sophorolipid is obtained by inoculating Candida albicans with glucose and vegetable oil as substrates, fermenting under sterile conditions, and then separating and purifying it; the preparation method of the rhamnosyl ester is as follows: inoculating Pseudomonas microorganisms into carbon source and vegetable oil, fermenting under sterile conditions, and then separating and purifying it. The Pseudomonas microorganisms can be Pseudomonas aeruginosa or Pseudomonas putida; the carbon source is one of glucose, glycerol, and citric acid.

3. The composition according to claim 1, characterized in that, The lipopeptide is a type of biosurfactant composed of β-amino or β-hydroxy fatty acids and peptide chains or peptide rings; more specifically, the lipopeptide is sodium subtilis lipopeptide. The plant saponin is a natural nonionic surfactant composed of saponins, sugars, uronic acids or other organic acids; preferably, the plant saponin is tea saponin.

4. A fully bio-based feminine hygiene gel product, characterized in that, It contains the composition as described in any one of claims 1 to 3 as a surfactant; Preferably, the all-bio-based feminine hygiene gel product further includes one or more of the following: thickener, moisturizer, preservative, conditioning agent, buffer, and fragrance.

5. The all-bio-based feminine hygiene gel product as described in claim 4, characterized in that, The thickener is hydroxyethyl cellulose or xanthan gum; the moisturizer is glycerin or sorbitol; the preservative is methylparaben or polylysine; the conditioning agent is plant hydrosol and niacinamide; and the buffer is citric acid.

6. The all-bio-based feminine hygiene gel product as described in claim 4, characterized in that, The composition, by weight percentage, is: 10.0-15.0% glycerol, 4.0-6.0% sorbitol, 0.8-1.0% hydroxyethyl cellulose, 0.10-0.15% methylparaben, 0.50-1.20% the composition according to any one of claims 1 to 3; the balance being water; Alternatively, it may be composed of: 6.0-8.0% glycerol, 0.50-0.80% xanthan gum, 0.10-0.20% polylysine, 0.50-1.20% of the composition as described in any one of claims 1 to 3, 0.20%-0.30% nicotinamide, 0.03-0.05% citric acid; the balance being plant hydrosol.

7. The method for preparing the fully bio-based feminine hygiene gel product according to any one of claims 4 to 6, characterized in that, Includes the following steps: Mix the humectant and preservative and heat to 45-55℃; Add thickener until the powder is completely dispersed and free of lumps; Add the humectant, surfactant, and active ingredient while maintaining a temperature of 40-50℃, and continue stirring for 30-90 minutes. After stirring, keep the gel at a high temperature of 85-90℃ for 5-15 minutes.

8. The method for preparing the all-bio-based feminine hygiene gel product as described in claim 7, characterized in that, The process includes the following steps: Glycerin and methylparaben are mixed and heated to 50°C; hydroxyethyl cellulose is added until the powder is completely dispersed and free of lumps, and the mixture is kept at 45°C. Sorbitol, sophorolipid Ecolife SL-02, tea saponin, and water are then added, and the mixture is stirred for 60 minutes. The resulting gel is then kept at 85-90°C for 5-15 minutes, hot-filled into bottles, and the pH is measured to be 5.

50.

9. The method for preparing the all-bio-based feminine hygiene gel product as described in claim 7, characterized in that, The process includes the following steps: Glycerin and methylparaben are mixed and heated to 50°C; hydroxyethyl cellulose is added until the powder is completely dispersed and free of lumps, and the mixture is kept at 45°C. Sorbitol, sophorolipid Ecolife SL-03, tea saponin, and water are then added, and the mixture is stirred for 60 minutes. The resulting gel is then kept at 85-90°C for 5-15 minutes, hot-filled into bottles, and the pH is measured to be 5.

65.

10. The method for preparing the all-bio-based feminine hygiene gel product as described in claim 7, characterized in that, The process includes the following steps: first, glycerol is used to wet and disperse xanthan gum, then plant hydrosol, lactone-type sophorolipid, acid-type sophorolipid, sodium subtilis lipopeptide, tea saponin, nicotinamide, and polylysine are added in sequence. Finally, citric acid is added to adjust the pH. The resulting gel is kept at a high temperature of 85-90℃ for 5-15 minutes, then hot-filled into bottles, and the pH is measured to be 6.20.

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