Tobacco bud inhibition composition

Abstract

The invention belongs to the technical field of agricultural compositions, and relates to a tobacco bud inhibition composition. The invention discloses a tobacco bud inhibition composition. Core components of the tobacco bud inhibition composition are flumetralin and quinclorac. The flumetralin and the quinclorac are mixed according to the mass ratio of (0.25-12): (0.005-0.09), the flumetralin and the quinclorac have a synergistic effect, and the bud inhibition effect is superior to that of a single agent after the flumetralin and the quinclorac are mixed. Field trials further verify that when flumetralin and quinclorac are mixed, the mass ratio of flumetralin to quinclorac is within the range of (0.25-12): (0.005-0.09), the bud inhibition effect is better than that of a control group, and no phytotoxicity phenomenon is found after pesticide application. According to the tobacco bud inhibition composition provided by the invention, the bud inhibition effect is improved while the pesticide consumption is reduced, and the tobacco bud inhibition composition which is high in safety and good in bud inhibition effect is provided for the market.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of agricultural compositions, and relates to a tobacco bud inhibition composition. BACKGROUND

[0002] In order to reduce the nutrient consumption in tobacco leaves and improve the yield and quality of tobacco leaves, the tobacco plants need to be topped in time and the buds need to be completely removed in the late growth period. However, due to the labor-intensive and time-consuming and incomplete removal of the buds by manual removal, since the 1940s, tobacco bud inhibitors have been widely used and applied in tobacco bud inhibition. At present, the widely used bud inhibitors in the tobacco planting areas mainly include 12.5% flumetralin emulsion and 36% butralin emulsion. These bud inhibitors mainly belong to dinitroaniline pesticides, and have certain bud inhibition effect, but also have problems in actual application. For example, due to the influence of factors such as air temperature, field humidity, tobacco plant growth uniformity and maturity, and tobacco grower's application technology, the bud inhibition effect is uneven. In order to improve the bud inhibition effect, some applicators often increase the application amount, which leads to high pesticide residues in the harvested tobacco leaves, and affects the quality and safety of the tobacco leaves. Therefore, it has become a technical problem to be solved that a safe and good bud inhibition composition for tobacco is further researched and selected.

[0003] Flumetralin, chemical name: N-(2-chloro-fluorobenzyl)-N-ethyl-α, α, α-trifluoro-2, 6-dinitro-p-toluidine, is a low-toxicity plant growth regulator, which has a high-efficiency effect of inhibiting the growth of tobacco lateral buds by contact and local internal absorption. The bud inhibition effect is rapid, absorption is fast, and the bud inhibition effect is good. However, although flumetralin has many advantages, it still needs to be combined with other chemical substances in actual application, in order to further improve the bud inhibition effect and safety.

[0004] Quinclorac, chemical name: 3, 7-dichloro-8-quinolinecarboxylic acid, is a pre- and post-emergence herbicide. Quinclorac can cause the increase of ACC oxidase of plants, and then catalyze the conversion of ACC into ethylene. With the increase of ethylene content, the byproduct cyanide also increases a lot, and cyanide is toxic to plants and can inhibit plant growth. Studies have shown that quinclorac has a great influence on tobacco, including a great inhibition effect on the leaf width and the length of the seedling roots of tobacco. In the rotation field, the phenomenon of phytotoxicity of tobacco caused by the herbicide residues of the previous crop is relatively common, especially in the rice and tobacco rotation production area, quinclorac can easily cause the growth of tobacco to be abnormal, resulting in a large decrease in yield and quality. Therefore, there is no report on the application of quinclorac in tobacco bud inhibition.

[0005] The skilled person in the art cannot know whether flumetralin and quinclorac can be combined and applied in tobacco bud inhibition without a large number of experiments, and it is unpredictable whether the combination of the two has a synergistic effect or an antagonistic effect. SUMMARY

[0006] In order to solve the problems of safety and effectiveness of the current tobacco bud inhibitor, the present application explores to find a compound which can be compounded with flumetralin to improve the bud inhibition effect of flumetralin and reduce the use amount of flumetralin, and the compound is mixed with flumetralin to form a new type of tobacco bud inhibition composition, so as to improve the safety and effectiveness of tobacco bud inhibition.

[0007] In one aspect, the present application provides a tobacco bud inhibition composition, characterized in that the core component of the tobacco bud inhibition composition is composed of flumetralin and quinclorac.

[0008] Further, the mass ratio of the flumetralin to the quinclorac is 0.25-12:0.005-0.09.

[0009] Further, the mass ratio of the flumetralin to the quinclorac is 1-8:0.02-0.06.

[0010] Further, the mass ratio of the flumetralin to the quinclorac is 4:0.04.

[0011] In another aspect, the present application also provides an agricultural composition containing the tobacco bud inhibition composition.

[0012] In still another aspect, the present application also provides a method for inhibiting tobacco axillary buds, which comprises the step of applying the tobacco bud inhibition composition capable of inhibiting tobacco axillary buds.

[0013] Further, the tobacco bud inhibition composition is a wettable powder.

[0014] Further, the tobacco bud inhibition composition is a water dispersible granule.

[0015] Further, the tobacco bud inhibition composition is a suspoemulsion.

[0016] Further, the tobacco bud inhibition composition is a water emulsion.

[0017] Compared with the prior art, the technical scheme provided by the present application at least has the following beneficial effects or advantages: This invention involves a mixture of flubendiamide and quinclorac. In pot experiments, a synergistic effect was observed between the two in a mass ratio of 0.25–12:0.005–0.09. The combined effect of both was superior to that of either agent in inhibiting tobacco axillary bud growth. The optimal synergistic effect, with a bud inhibition rate of 100%, was achieved when the mass ratio of flubendiamide to quinclorac was 4:0.04. Field trial results were consistent with the pot experiment results. A mass ratio of flubendiamide to quinclorac in a mass ratio of 0.25–12:0.005–0.09 showed better inhibition of tobacco axillary bud growth than the control group. Furthermore, the optimal field bud inhibition rate (91.21%) was achieved when the mass ratio of flubendiamide to quinclorac was 4:0.04. Furthermore, the amount of flubendiamide used in the tobacco bud-suppressing composition provided by this invention is significantly reduced. In particular, when flubendiamide and dichloroquinoline acid are mixed in a mass ratio of 4:0.04, the amount of flubendiamide used is only 5% of that in the control group, and the tobacco bud-suppressing effect reaches 91.21%, far superior to the control. This indicates that the present invention effectively reduces the amount of flubendiamide used while improving the tobacco bud-suppressing effect. This invention provides a safe and effective bud-suppressing composition for tobacco bud suppression. Detailed Implementation

[0018] The technical solution of the present invention will be described below with reference to the embodiments. However, the present invention is not limited to the following embodiments.

[0019] To enable those skilled in the art to better understand and implement the technical solutions of the present invention, the present invention will be further described below with reference to specific embodiments, but the embodiments are not intended to limit the present invention.

[0020] Unless otherwise specified, the experimental and detection methods described in the following embodiments are conventional methods; unless otherwise specified, the reagents and materials are commercially available.

[0021] Example 1 This example is a test experiment on the inhibitory effect of a mixture of flubendiamide and dichloroquinoline acid on tobacco sprouting.

[0022] Tobacco variety tested: Yunyan 87.

[0023] Test reagents: Flubenzamide and dichloroquinoline acid.

[0024] Treatment of test reagents: Dissolve the test reagents flutriafol and dichloroquinoline acid in acetone first, then dilute them with 0.1% Tween-80 aqueous solution to prepare single-agent stock solutions and stock solutions of each proportioned mixture. Set different mass concentration gradients for both single-agent and proportioned mixtures for later use.

[0025] Dilute the above-mentioned mixed solutions and single-agent stock solutions of different concentrations with water at a ratio of 1:1000 and set aside for later use.

[0026] The test set up a water group (blank control group), set up a single dose group of the test agent, and a mixed agent group, i.e. a tobacco bud inhibition agricultural composition test group.

[0027] An indoor pot experiment was conducted, and tobacco plants at the 8-9 true leaf stage were treated with low temperature (6-10℃) for 1200-1600h to induce early flowering. After the flower buds were removed, different proportions of flumetralin and quinclorac mixtures and single agents were applied by intercalary dripping, and clear water was applied as a blank control group (CK). Eight replicates were set for each treatment, and the number of axillary buds of tobacco was counted 40 days after application. The bud inhibition rate was calculated.

[0028] For example, test group 1 was prepared by dissolving 0.1g of flumetralin and 0.1g of quinclorac in acetone, and then diluting with 0.1% Tween-80 aqueous solution to a total mass of 100.00g. The flumetralin:quinclorac ratio was 0.1:0.1 by mass. The tobacco bud inhibition composition of test group 1 was diluted with water at a ratio of 1:1000, and then applied by intercalary dripping to the tobacco. The number of axillary buds of the tobacco treated with test group 1 was counted 40 days after application, and the bud inhibition rate was calculated.

[0029] The Gowing method was used to evaluate the effect of the mixture of each active ingredient on the bud inhibition rate of tobacco. The formula is E0=X+[Y×(100-X) / 100], E0 represents the theoretical bud inhibition rate of the composition (A+B) of tobacco when the dosage is (P+Q), X represents the bud inhibition rate of agent A of tobacco when the dosage is P, Y represents the bud inhibition rate of agent B of tobacco when the dosage is Q, and E represents the actual bud inhibition rate of the composition. The test results of the effects of different treatments on the bud inhibition of tobacco are shown in Table 1.

[0030] When E-E0>10%, it is a synergistic effect; when E-E0<-10%, it is an antagonistic effect; and when E-E0 is between ±10%, it is an additive effect.

[0031] Bud inhibition rate (%) = (number of axillary buds of the blank control group - number of axillary buds of the treated group) / number of axillary buds of the blank control group × 100 Table 1 Test results of the effects of different treatments on the bud inhibition of tobacco

[0032] The results in Table 1 show that the mixture of flumetralin and quinclorac in a mass ratio of 0.25-12:0.005-0.09 has a synergistic effect on the bud inhibition of tobacco. In particular, the mixture of flumetralin and quinclorac in a mass ratio of 1-8:0.02-0.06 has a significant synergistic effect on the bud inhibition of tobacco. The mixture of flumetralin and quinclorac in a mass ratio of 4:0.04 has the best synergistic effect on the bud inhibition of tobacco, and the bud inhibition rate of tobacco reaches 100%.

[0033] Example 2 This example is to prepare a tobacco bud control composition wettable powder.

[0034] Take flumetralin 0.25g, quinclorac 0.09g, polycarboxylate 10.00g, soapstone powder 10.00g, the rest is supplemented with kaolin to 100.00g. The above raw materials are mixed uniformly in a mixing cylinder, crushed by an air flow crusher, and then mixed uniformly, to prepare a tobacco bud control composition wettable powder. Marked as 1# tobacco bud control composition.

[0035] Example 3 This example is to prepare a tobacco bud control composition wettable powder.

[0036] Take flumetralin 0.50g, quinclorac 0.07g, lignin sulfonate 12.00g, sodium alginate 10.00g, the rest is supplemented with penguin soil to 100.00g. The above raw materials are mixed uniformly in a mixing cylinder, crushed by an air flow crusher, and then mixed uniformly, to prepare a tobacco bud control composition wettable powder. Marked as 2# tobacco bud control composition.

[0037] Example 4 This example is to prepare a tobacco bud control composition water dispersible granule.

[0038] Take flumetralin 1.00g, quinclorac 0.06g, lignin sulfonate 15.00g, sodium dodecyl sulfonate 12.00g, urea 2.00g, the rest is supplemented with starch to 100.00g. The above flumetralin, quinclorac, lignin sulfonate, sodium dodecyl sulfonate, and urea are air flow crushed together, and then mixed with starch to prepare granulation material. The granulation material is quantitatively sent into a fluidized bed granulation dryer for granulation and drying. A tobacco bud control composition water dispersible granule is prepared. Marked as 3# tobacco bud control composition.

[0039] Example 5 This example is to prepare a tobacco bud control composition water dispersible granule.

[0040] Take flumetralin 2.00g, quinclorac 0.05g, fatty acid polyoxyethylene ester 15.00g, sodium dodecyl sulfonate 15.00g, glucose 3.00g, the rest is supplemented with starch to 100.00g. The above flumetralin, quinclorac, fatty acid polyoxyethylene ester, sodium dodecyl sulfonate, and glucose are air flow crushed together, and then mixed with starch to prepare granulation material. The granulation material is quantitatively sent into a fluidized bed granulation dryer for granulation and drying. A tobacco bud control composition water dispersible granule is prepared. Marked as 4# tobacco bud control composition.

[0041] Example 6 This embodiment describes the preparation of a tobacco bud-inhibiting composition suspension emulsion.

[0042] Weigh out 4.00g of flubenzamide, 0.04g of dichloroquinoline acid, 15.00g of lignin sulfonate, 15.00g of sodium dodecylbenzenesulfonate, 8.00g of glycerin, 2.00g of silicone oil, and 1.00g of xanthan gum. Make up the remainder with deionized water to a total of 100.00g. Mix the weighed lignin sulfonate, sodium dodecylbenzenesulfonate, glycerin, xanthan gum, and silicone oil evenly using a high-speed shearing method. Add flubenzamide and dichloroquinoline acid, then add deionized water. Ball mill the mixture for 2-3 hours until the particle size is less than or equal to 6μm. This yields a tobacco bud-suppressing composition suspension emulsion, labeled as Tobacco Bud-suppressing Composition #5.

[0043] Example 7 This embodiment describes the preparation of a tobacco bud-inhibiting composition suspension emulsion.

[0044] Weigh out 6.00g of fluphenazine, 0.03g of dichloroquinoline acid, 12.00g of fatty alcohol polyoxyethylene ether, 10.00g of sodium lauryl sulfate, 6.00g of propylene glycol, 2.00g of silicone oil, and 2.00g of sodium tripolyphosphate. Make up the remainder with deionized water to a total of 100.00g. Mix the weighed fatty alcohol polyoxyethylene ether, sodium lauryl sulfate, propylene glycol, silicone oil, and sodium tripolyphosphate evenly using a high-speed shearing method. Add fluphenazine and dichloroquinoline acid, then add deionized water. Ball mill the mixture for 2-3 hours until the particle size is less than or equal to 6μm. This yields a tobacco bud-suppressing composition suspension emulsion, labeled as Tobacco Bud-suppressing Composition #6.

[0045] Example 8 This embodiment describes the preparation of a water-based emulsion of a tobacco bud-inhibiting composition.

[0046] Weigh out 8.00g of fluphenazine, 0.02g of dichloroquinoline acid, 30.00g of mineral oil, 10.00g of triethanolamine salt, 8.00g of propylene glycol, 2.00g of methylcellulose, 1.00g of decanoic acid, and make up the remainder with deionized water to 100.00g.

[0047] The weighed fluphenazine, dichloroquinoline acid, triethanolamine salt, and mineral oil were mixed and dissolved to form a homogeneous oil phase; deionized water and the remaining raw materials were mixed to form a homogeneous aqueous phase. The aqueous phase was added to the oil phase under high-speed stirring. This yields a tobacco bud-suppressing composition emulsion, labeled as Tobacco Budding Suppressing Composition No. 7.

[0048] Example 9 This embodiment describes the preparation of a water-based emulsion of a tobacco bud-inhibiting composition.

[0049] Flumetralin 10.00 g, quinclorac 0.01 g, mineral oil 31.00 g, triethanolamine salt 8.00 g, propylene glycol 10.00, methyl cellulose 1.00 g, capric acid 2.00 g, and the rest is made up with deionized water to 100.00 g.

[0050] The weighed flumetralin, quinclorac, triethanolamine salt and mineral oil are mixed and dissolved to form a uniform oil phase; the deionized water and the remaining raw materials are mixed to form a uniform water phase. Under high-speed stirring, the water phase is added to the oil phase. Thus, a tobacco bud inhibition composition emulsion is prepared. It is labeled as 8# tobacco bud inhibition composition.

[0051] Example 10 This example is for preparing a tobacco bud inhibition composition emulsion.

[0052] Flumetralin 12.00 g, quinclorac 0.005 g, mineral oil 30.00 g, triethanolamine salt 9.00 g, propylene glycol 10.00, methyl cellulose 1.00 g, capric acid 2.00 g, and the rest is made up with deionized water to 100.00 g.

[0053] The weighed flumetralin, quinclorac, triethanolamine salt and mineral oil are mixed and dissolved to form a uniform oil phase; the deionized water and the remaining raw materials are mixed to form a uniform water phase. Under high-speed stirring, the water phase is added to the oil phase. Thus, a tobacco bud inhibition composition emulsion is prepared. It is labeled as 9# tobacco bud inhibition composition.

[0054] Example 11 This example is for testing the field bud inhibition effect of a tobacco bud inhibition composition.

[0055] The tested tobacco variety is Yunyan 87.

[0056] The tested samples are 1#-9# tobacco bud inhibition compositions.

[0057] The control sample is 12.5% flumetralin.

[0058] The blank control sample is water The normal tobacco plants after topping are used as the test material. 12.5% flumetralin (diluted 300 times) and 1#-9# tobacco bud inhibition compositions (diluted 1000 times with water) are used to test the bud inhibition effect according to the conventional method. The cup drenching method is used for application, and the amount applied per plant is 20 mL. Other field management is conventional management. Three points are taken from each plot after application, and 10 tobacco plants are taken from each point. The axillary bud fresh weight is investigated one week after application, and the tobacco axillary bud inhibition effect is calculated. The bud inhibition effect calculation formula is as follows: Bud inhibition effect (%) = (axillary bud fresh weight of blank control area - axillary bud fresh weight of application area) / axillary bud fresh weight of blank control area x 100 The results of the tobacco bud inhibition test of different treatments are shown in Table 2. In addition, after the application of the pesticides, the adverse effects of the different pesticides on the growth of tobacco were continuously observed, and the adverse effects were observed according to the growth inhibition, necrosis, discoloration, and deformation of the leaves (upper 1-4 leaves).

[0059] Table 2 Statistical results of the tobacco bud inhibition effect of different treatments

[0060] The results show that the tobacco bud inhibition effect of the tobacco bud inhibition compositions 1#-9# prepared by mixing flumetralin and quinclorac in the core component is better than that of flumetralin, and the amount of flumetralin (1:1000 dilution) in the tobacco bud inhibition compositions in this embodiment is less than that of the control group flumetralin (1:300 dilution), especially the amount of flumetralin in the 5# tobacco bud inhibition composition is only 5% of that of the control group flumetralin, and the inhibition effect on tobacco axillary buds reaches 91.21%, which is much better than the inhibition effect of 54.29% of the control group, indicating that the tobacco bud inhibition compositions prepared by the application can reduce the amount of flumetralin while improving the bud inhibition effect of flumetralin. The 5# tobacco bud inhibition composition in the embodiment of the application has the best bud inhibition effect, which is the best embodiment of the application.

[0061] In addition, the adverse effects of 1#-9# tobacco bud inhibition compositions and the control group on the growth of tobacco were continuously observed after the application of the pesticides, and no adverse effects on the growth of tobacco were found in 1#-9# tobacco bud inhibition compositions and the control group, and no phytotoxicity was found, and the width of the tobacco leaves in the application area of 1#-9# tobacco bud inhibition compositions was slightly wider than that of the control group. It is shown that the 1#-9# tobacco bud inhibition compositions of the application significantly improve the bud inhibition effect of tobacco while being harmless to the growth of tobacco.

[0062] As described above, the basic principles, main features, and advantages of the application are better described. The above examples and descriptions are only for describing the preferred embodiments of the application, and the application is not limited by the above examples. Without departing from the spirit and scope of the application, various changes and improvements to the technical solutions of the application made by those skilled in the art shall fall within the scope of protection of the application.

Claims

1. A tobacco bud-inhibiting composition, characterized in that, The core components of the tobacco bud-suppressing composition consist of flubenzamide and dichloroquinoline acid.

2. The tobacco bud-suppressing composition according to claim 1, characterized in that, The mass ratio of fluoroquinolone to dichloroquinoline acid is 0.25~12:0.005~0.

09.

3. The tobacco bud-suppressing composition according to claim 2, characterized in that, The mass ratio of fluoroquinolone to dichloroquinoline acid is 1~8:0.02~0.

06.

4. The tobacco bud-inhibiting composition according to claim 3, characterized in that, The mass ratio of fluoroquinolone to dichloroquinoline acid is 4:0.

04.

5. An agricultural composition, characterized in that, The agricultural composition contains the tobacco bud-inhibiting composition of claim 1.

6. A method for inhibiting tobacco axillary buds, characterized in that, The method includes the step of applying the tobacco bud-inhibiting composition according to any one of claims 1 to 4.

7. The method according to claim 6, characterized in that, The tobacco bud-suppressing composition is a wettable powder.

8. The method according to claim 6, characterized in that, The tobacco bud-inhibiting composition is a water-dispersible granule.

9. The method according to claim 6, characterized in that, The tobacco bud-inhibiting composition is a suspension emulsion.

10. The method according to claim 6, characterized in that, The tobacco bud-inhibiting composition is an emulsion.

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