Bacillus amyloliquefaciens QGFCH3-3 as well as fungicide and application thereof

Abstract

The invention belongs to the technical field of microorganisms, and particularly discloses bacillus amyloliquefaciens QGFCH3-3 as well as a microbial agent and application of the bacillus amyloliquefaciens QGFCH3-3. The preservation number of the bacillus amyloliquefaciens QGFCH3-3 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.34814 The strain has the functions of nitrogen fixation, amylase production, ligninase production, protease production and cellulase production, can effectively promote plant growth, has broad-spectrum antibacterial activity, can significantly inhibit various pathogenic bacteria, and significantly improves the disease control effect of crops.

Description

Technical Field

[0001] This invention relates to the field of microbial technology, and in particular to a Bacillus amyloliquefaciens QGFCH3-3, its inoculum, and its applications. Background Technology

[0002] Plant fungal diseases are one of the major threats to agricultural production, accounting for approximately 70% to 80% of all plant diseases. Almost every crop is susceptible to multiple fungal diseases. Currently, the control of plant fungal diseases still relies primarily on chemical agents. However, the long-term and extensive use of chemical agents has led to a series of problems: on the one hand, chemical residues remain in soil, water, and the atmosphere; on the other hand, pathogens easily develop resistance to chemical agents, resulting in a gradual decline in control effectiveness, necessitating increased pesticide application and creating a vicious cycle; furthermore, pesticide residues in agricultural products directly threaten human health, contradicting the development concepts of green agriculture and food safety. Therefore, finding environmentally friendly and sustainable alternatives to chemical agents has become an urgent need to promote the green and efficient development of agriculture.

[0003] Among numerous biological control resources, Bacillus bacteria have become a focus of attention due to their unique biological characteristics and functional advantages. These bacteria are widely distributed in nature, easy to isolate and culture, and characterized by rapid growth and active metabolism, facilitating large-scale production. Furthermore, they exhibit strong resilience, tolerating extreme environments such as high temperatures, drought, and acidity / alkalinity. They can stably survive and function in complex environments such as soil and plant surfaces, and possess high biosafety, posing no harm to humans, livestock, crops, or the ecological environment.

[0004] However, existing commercially available Bacillus strains still have certain limitations in practical applications: some strains have a narrow antibacterial spectrum, resulting in unsatisfactory control effects against specific pathogens; the colonization ability and stress resistance of some strains in complex field environments need to be improved, leading to insufficient stability of control effects; in addition, the production processes of some strains are complex and costly, limiting their large-scale promotion and application. Therefore, screening new Bacillus strains with broader antibacterial activity, stronger environmental adaptability, and higher application efficiency, and optimizing their application technologies, is of great significance for improving the level of biological control and promoting green agricultural development. Summary of the Invention

[0005] In view of the above, it is necessary to provide a strain that can fix nitrogen, produce amylase, ligninase, protease and cellulase, and has broad-spectrum antibacterial activity, and can significantly inhibit a variety of pathogens, so as to promote green production and quality improvement in agriculture.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0007] A strain of Bacillus amyloliquefaciens, QGFCH3-3, classified as Bacillus amyloliquefaciens, is deposited at the China General Microbiological Culture Collection Center (CGMCC) at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, on June 9, 2025, with accession number CGMCC No. 34814.

[0008] Furthermore, the Bacillus amyloliquefaciens QGFCH3-3 has at least one of the following functions: nitrogen fixation, amylase production, lignin production, protease production, and cellulase production.

[0009] The present invention also provides a bacterial agent comprising the aforementioned Bacillus amyloliquefaciens QGFCH3-3 or its metabolites.

[0010] The present invention also provides the application of the Bacillus amyloliquefaciens QGFCH3-3 or the bacterial agent in the prevention and control of plant diseases caused by pathogens, including anthracnose, gray mold, wilt, sheath blight, Fusarium head blight, white spot disease and scab.

[0011] Furthermore, the plant diseases mentioned include: banana anthracnose, mango anthracnose, tomato gray mold, cotton wilt, rice sheath blight, wheat scab, banana wilt, pepper white spot disease, and potato scab.

[0012] Furthermore, the pathogens include: *Colletotrichum musae*, *Colletotrichum gloeosporioides penz*, *Colletotrichum asianum*, *Colletotrichum siamense*, *Colletotrichum fructicola*, *Botrytis cinerea*, *Fusarium oxysporum*, *Rhizoctonia solani*, *Fusarium graminearum*, *Fusarium asiaticum*, *Phyllosticta capsici*, and *Streptomyces*.

[0013] The present invention also provides the application of the Bacillus amyloliquefaciens QGFCH3-3 or the bacterial agent in the field control of mango anthracnose. The field control method for mango anthracnose includes the following steps: at the early stage of anthracnose in mangoes, the bacterial agent is sprayed on the mango plants, and the spraying is repeated 3 times, with an interval of 10-12 days between each application.

[0014] Furthermore, the bacterial agent is a wettable powder of Bacillus amyloliquefaciens QGFCH3-3, which comprises the following raw material components by mass percentage: 58.5% fermentation broth of Bacillus amyloliquefaciens QGFCH3-3, 30% carrier, 6.5% dispersant, 3.5% wetting agent, and 1.5% stabilizer; the carrier is made by mixing diatomaceous earth and kaolin in a mass ratio of 4:1, the dispersant is Tween-60, the wetting agent is sodium dodecyl sulfate, and the stabilizer is humic acid.

[0015] The present invention also provides the application of the Bacillus amyloliquefaciens QGFCH3-3 or the bacterial agent thereon in promoting plant growth or promoting plant growth under salt stress.

[0016] The present invention has the following beneficial effects:

[0017] 1. The *Bacillus amyloliquefaciens* QGFCH3-3 of this invention possesses nitrogen-fixing, amylase-producing, lignin-producing, protease-producing, and cellulase-producing functions, thereby decomposing organic matter in the soil, promoting the transformation of soil organic matter, decomposing organic matter into plant-usable mineral elements, participating in N2 fixation, and participating in nitrification and denitrification processes. Furthermore, this strain has an optimal pH range of 6.0-9.5 for growth and can grow under conditions of 10-12% salinity (NaCl mass concentration). Therefore, applying it to the soil helps activate soil nutrients, improves the alkali and salt tolerance of plants, and thus promotes plant growth.

[0018] 2. The Bacillus amyloliquefaciens QGFCH3-3 of the present invention and the bacterial agent containing the strain or its metabolites have broad-spectrum antibacterial activity, can significantly inhibit a variety of pathogens, significantly reduce the incidence of diseases in a variety of crops, and improve the control effect, especially significantly improve the control effect of mango anthracnose, and have great value in crop application. Attached Figure Description

[0019] Figure 1 This is a colony diagram of the Bacillus amyloliquefaciens QGFCH3-3 strain on a plate, representing the bacterial colony of this invention.

[0020] Figure 2This is a microscopic image of the Bacillus amyloliquefaciens QGFCH3-3 strain of the present invention.

[0021] Figure 3 The diagram shows the amylase production capacity of Bacillus amyloliquefaciens QGFCH3-3, which is the subject of this invention.

[0022] Figure 4 The diagram shows the lignin-producing capacity of Bacillus amyloliquefaciens QGFCH3-3, which is the present invention.

[0023] Figure 5 This is a graph showing the protease production capacity of Bacillus amyloliquefaciens QGFCH3-3, which is the subject of this invention.

[0024] Figure 6 This is a graph showing the cellulase production capacity of Bacillus amyloliquefaciens QGFCH3-3, which is the subject of this invention.

[0025] Information on the preservation of biological materials

[0026] The strain information deposited in this application is: Bacillus amyloliquefaciens QGFCH3-3, classified as Bacillus amyloliquefaciens, deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, on June 9, 2025, with accession number CGMCC No. 34814. Detailed Implementation

[0027] To make the technical problems, technical solutions and advantages of the present invention clearer, a detailed description will be given below in conjunction with specific embodiments.

[0028] All features disclosed in this specification, or all steps in all disclosed methods or processes, may be combined in any way, except for mutually exclusive features and / or steps.

[0029] Unless otherwise stated, each feature disclosed in this specification (including any appended claims and abstract) is merely one example of a series of equivalent or similar features.

[0030] Unless otherwise specified, all materials and reagents used in the following examples are commercially available.

[0031] In the following examples, the culture medium used is as follows:

[0032] LB liquid medium: 10.0 g peptone, 5.0 g yeast extract, 5.0 g sodium chloride, 1000 mL pure water, pH adjusted to 7.0 ± 0.1 with sodium hydroxide, sterilized at 121℃ for 20 min.

[0033] LB solid medium: 18g agar, 10.0g peptone, 5.0g yeast extract, 5.0g sodium chloride, 1000mL pure water, pH adjusted to 7.0±0.1 with sodium hydroxide, sterilized at 121℃ for 20min.

[0034] Example 1

[0035] This embodiment provides a Bacillus amyloliquefaciens strain QGFCH3-3, which was isolated from a salt sample of Emerald Lake in Da Qaidam, Qinghai Province. The specific isolation and screening method is as follows:

[0036] (1) Strain isolation and screening

[0037] The salt sample was collected from Emerald Lake in Da Qaidam, Qinghai Province, and brought back to the laboratory in a sterile bag, where it was stored at 4℃. 5g of the salt sample was weighed and added to 45mL of sterile water to prepare a concentration of 10. -1 The soil-sampled bacterial suspension was diluted in 10-fold serial dilutions to achieve a bacterial suspension concentration of 10. -1 ~10 -7 After vortex oscillation and mixing, take 10 -3 10 -4 10 -5 Three gradients of 100 μL bacterial suspension were spread onto LB agar plates, with two replicates for each gradient, and incubated at 35°C for 24 h. Representative strains were selected based on colony color, elevation, edge shape, and size, and streaked onto LB agar plates, then incubated at 35°C. After two subcultures, strains with uniform morphology were Gram-stained and examined microscopically. Strains with uniform microscopic examination were confirmed as purified, numbered QGFCH3-3, and stored at -40°C to -80°C (final concentration 25% glycerol) for later use.

[0038] (2) Physiological and biochemical identification of colonies

[0039] Observe the morphology and color of the colonies grown by the strain on the surface of LB medium according to Bergey's Manual of Bacteriological Identification (9th Edition). The colony morphology diagram is shown below. Figure 1As shown; young cultures were selected, smeared, Gram-stained, and observed under a microscope for bacterial morphology, size, Gram staining reaction, and the presence, morphology, and attachment position of spores, etc. The microscopic images are shown below. Figure 2 As shown.

[0040] (3) Sequence analysis of the strain's 16S rRNA

[0041] The strain QGFCH3-3 was sent to the Sequencing and Identification Department of Shanghai Sangon Biotech for sequencing identification. Its sequence is shown below:

[0042]

[0043] The sequence was compared and analyzed in the NCBI ribosome database. 16S rRNA sequence analysis showed that the strain of this invention shared 100% homology with *Bacillus amyloliquefaciens*, meaning the strain is indeed *Bacillus amyloliquefaciens*.

[0044] The microorganisms in this embodiment were preserved as follows:

[0045] Bacillus amyloliquefaciens QGFCH3-3, classified as Bacillus amyloliquefaciens, is deposited at the China General Microbiological Culture Collection Center (CGMCC) at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The deposit date is June 9, 2025, and the accession number is CGMCC No. 34814.

[0046] Example 2

[0047] This embodiment measures the growth-promoting and enzyme-producing abilities of Bacillus amyloliquefaciens QGFCH3-3, as detailed below:

[0048] (1) Preparation of Bacillus amyloliquefaciens QGFCH3-3 bacterial culture: Bacillus amyloliquefaciens QGFCH3-3 was inoculated into LB liquid medium and cultured at 30℃ and 180r / min with shaking until OD600≈1.0, thus obtaining the Bacillus amyloliquefaciens QGFCH3-3 bacterial culture.

[0049] (2) Preparation of single colonies of Bacillus amyloliquefaciens QGFCH3-3: Bacillus amyloliquefaciens QGFCH3-3 was inoculated on LB solid medium and cultured in three zones for 1 day to obtain single colonies of Bacillus amyloliquefaciens QGFCH3-3.

[0050] (3) Nitrogen fixation capacity test: Single colonies of Bacillus amyloliquefaciens QGFCH3-3 were streaked in three zones and inoculated onto nitrogen-fixing medium. The culture was carried out at 30℃ for 3 days, and the growth of Bacillus amyloliquefaciens QGFCH3-3 on nitrogen-fixing medium was observed. The results showed that Bacillus amyloliquefaciens QGFCH3-3 could grow normally on nitrogen-fixing medium.

[0051] (4) Amylase production capacity assay: Bacillus amyloliquefaciens QGFCH3-3 bacterial suspension was added to amylase detection medium and incubated at 30℃ for 7 days. A small amount of Lugol's iodine solution was added to the plate, and the presence of a colorless transparent zone around the bacterial growth was observed. Results were as follows: Figure 3 As shown in (a), Bacillus amyloliquefaciens QGFCH3-3 can produce a clear zone in the amylase detection medium, indicating that Bacillus amyloliquefaciens QGFCH3-3 has the ability to produce amylase.

[0052] (5) Assay for lignin-degrading enzyme production: Bacillus amyloliquefaciens QGFCH3-3 bacterial suspension was added dropwise to an aniline blue decolorizing plate and incubated at 27℃. Observations were made daily, and the presence of lignin-degrading enzymes was determined by whether a decolorizing zone appeared around the colonies in the aniline blue medium. Results were as follows: Figure 4 As shown in (a), Bacillus amyloliquefaciens QGFCH3-3 can produce decolorization on aniline blue decolorization plates, indicating that Bacillus amyloliquefaciens QGFCH3-3 has the ability to produce ligninase.

[0053] (6) Protease production capacity assay: Bacillus amyloliquefaciens QGFCH3-3 was inoculated into skim milk powder plates and cultured at 37℃ for 72 h. The presence of a colorless transparent zone around the bacterial colony was then observed. Results are as follows: Figure 5 As shown in (a), Bacillus amyloliquefaciens QGFCH3-3 can produce a clear zone, indicating that Bacillus amyloliquefaciens QGFCH3-3 has the ability to produce protease.

[0054] (7) Cellulase production capacity assay: *Bacillus amyloliquefaciens* QGFCH3-3 bacterial suspension was inoculated into Congo red solid medium and cultured at 30℃ for 5 days. The presence of a clear halo around the colonies was observed. Results were as follows: Figure 6 As shown in (a), Bacillus amyloliquefaciens QGFCH3-3 can produce a clear zone, indicating that Bacillus amyloliquefaciens QGFCH3-3 has the ability to produce cellulase.

[0055] Example 3

[0056] This embodiment measures the antibacterial activity of Bacillus amyloliquefaciens QGFCH3-3, as detailed below:

[0057] 1. The tested plant pathogenic fungi included: *Colletotrichum musae*, *Colletotrichum gloeosporioides penz*, *Colletotrichum asianum*, *Colletotrichum siamense*, *Colletotrichum fructicola*, *Botrytis cinerea*, *Fusarium oxysporum*, *Rhizoctonia solani*, *Fusarium graminearum*, *Fusarium asiaticum*, *Phyllosticta capsici*, and *Streptomyces*.

[0058] 2. Test method: A pathogenic bacterial cake with a diameter of 6 mm was inoculated in the center of a PDA medium plate. 5 μL of Bacillus amyloliquefaciens QGFCH3-3 bacterial suspension (prepared in Example 2) was inoculated 20 mm from the center of the plate. PDA medium containing only the pathogenic bacteria was used as a control. The plates were incubated at 28℃ for 5 days. The antibacterial effect of the strains was observed and the inhibition rate was calculated.

[0059] Inhibition rate (%) = (colon radius of control group - colony radius of treatment group) / colony radius of control group × 100%.

[0060] 3. Measurement Results

[0061] The antibacterial rate is shown in Table 1 below.

[0062] Table 1. Results of antibacterial assay of Bacillus amyloliquefaciens QGFCH3-3

[0063]

[0064] As shown in Table 1, Bacillus amyloliquefaciens QGFCH3-3 has an inhibitory effect on a variety of pathogens, with the highest inhibition rate reaching 91.34%.

[0065] Example 4

[0066] This embodiment measures the field control effect of Bacillus amyloliquefaciens QGFCH3-3 on mango anthracnose.

[0067] The Bacillus amyloliquefaciens QGFCH3-3 wettable powder used in this embodiment was formulated (by weight percentage) as follows after screening the carrier, wetting agent, dispersant, stabilizer, and their compounding ratios: 58.5% fermentation broth of Bacillus amyloliquefaciens QGFCH3-3, 30% carrier (diatomaceous earth and kaolin mixed at a weight ratio of 4:1), 6.5% dispersant (Tween-60), 3.5% wetting agent (sodium dodecyl sulfate), and 1.5% stabilizer (humic acid). The corresponding preparation method is as follows: the Bacillus amyloliquefaciens B15 fermentation broth is mixed with the carrier, then mixed with the dispersant, wetting agent, and stabilizer, pulverized and mixed thoroughly to prepare the Bacillus amyloliquefaciens QGFCH3-3 wettable powder.

[0068] This experiment was conducted in a mango orchard in Baise, Guangxi. The soil was moderately fertile, consisting of red soil, and the mango trees were 7 years old. The mango trees in the orchard were of roughly the same age, and the experimental mango variety was Jin Huang Mango. A pathogen was isolated from diseased mangoes in the experimental site using tissue isolation. The pathogen was identified as *Colletotrichum gloeosporioides* penz. Three treatments were used: 1000-fold dilution of 45% prochloraz emulsion, *Bacillus amyloliquefaciens* QGFCH3-3 wettable powder, and *Bacillus amyloliquefaciens* QGFCH3-3 fermentation broth. Each treatment was replicated three times. The infected mango trees in the orchard were randomly divided into four plots, each containing 10 mango trees. Three plots were sprayed with different pesticides, while one plot served as a control group without pesticide treatment. Spraying began at the early stage of anthracnose infection and was conducted three times consecutively, with 10-day intervals between each application. Each application continued until the pesticide solution formed droplets on the leaves but did not drip.

[0069] Before applying the pesticide, the occurrence of anthracnose in the orchard was investigated. Ten days after the third application, the anthracnose status of mangoes was investigated. A five-point sampling method was used, with five mango trees randomly selected from each plot to investigate the occurrence of anthracnose. The disease severity standards for mango anthracnose were as follows: using the number of brown spots on the mango fruit as the evaluation index, a disease level of 0 was defined as no lesions on the fruit; ≤5 lesions on the fruit indicated a disease level of 1; 5 < lesions ≤ 15 lesions on the fruit indicated a disease level of 3; 15 < lesions ≤ 25 lesions on the fruit indicated a disease level of 5; >25 lesions on the fruit indicated a disease level of 7; and densely distributed lesions with red dots indicated a disease level of 9. The efficacy calculation formula is as follows:

[0070]

[0071]

[0072] The prevention and control effects are shown in Table 2 below.

[0073] Table 2 Results of field efficacy trials for mango anthracnose

[0074]

[0075] As shown in Table 2, the fermentation broth of Bacillus amyloliquefaciens QGFCH3-3 of this invention achieved a field control effect of over 67% against mango anthracnose, indicating that Bacillus amyloliquefaciens QGFCH3-3 has a certain control effect against mango anthracnose in the field. Furthermore, the wettable powder of Bacillus amyloliquefaciens QGFCH3-3 achieved a field control effect of over 77% against mango anthracnose, demonstrating that the wettable powder of Bacillus amyloliquefaciens QGFCH3-3 can improve the control effect against mango anthracnose.

[0076] Example 5

[0077] This embodiment measures the growth-promoting effect of Bacillus amyloliquefaciens QGFCH3-3.

[0078] 1. Growth-promoting effect of Bacillus amyloliquefaciens QGFCH3-3 on potted cotton.

[0079] (1) Test crops and soils: cotton and Xinjiang cotton soil.

[0080] (2) Test sample: 500 times dilution of Bacillus amyloliquefaciens QGFCH3-3 bacterial suspension (prepared in Example 2).

[0081] (3) Experimental methods: The experiment consisted of two treatments: a treatment group (QH3-3) and a control group (CK), with five replicates per treatment (one replicate per pot). Each pot (13.7 cm inner diameter) contained one cotton plant, arranged randomly. Ten days after the cotton seedlings recovered from transplanting (3-4 leaf stage), the treatment group received 200 mL / pot of QGFCH3-3 bacterial solution diluted 500 times, applied to the roots at 7-day intervals, for a total of three treatments. The control group received an equal amount of water applied to the roots. All treatment groups were managed in the same manner, and during the second treatment, all treatment groups received 800 times diluted compound fertilizer (17-17-17). Samples were collected 17 days after the third treatment.

[0082] (4) Survey indicators and methods

[0083] The collected cotton plants were investigated using the survey indicators and methods shown in Table 3.

[0084] Table 3. Cotton Survey Indicators and Methods

[0085]

[0086] (5) Test results

[0087] The experimental results are shown in Table 4 below.

[0088] Table 4. Growth-promoting effects of QGFCH3-3 on potted cotton.

[0089]

[0090] As shown in Table 4, compared with the water group, the plant height, stem diameter, above-ground and underground parts of the potted cotton in the QGFCH3-3 group were significantly increased, with the highest increase reaching 59.74%, indicating that Bacillus amyloliquefaciens QGFCH3-3 has a significant growth-promoting effect on cotton.

[0091] 2. The effect of Bacillus amyloliquefaciens QGFCH3-3 on salt tolerance and growth promotion of potted peppers.

[0092] (1) Test crop: chili pepper (variety: La Shen F1).

[0093] (2) Test sample: Bacillus amyloliquefaciens QGFCH3-3 bacterial suspension (prepared in Example 2) 300 times dilution.

[0094] (3) Experimental methods: There were three treatments in the experiment: blank control group (CK0), salt treatment control group (CK), and bacterial solution treatment group (QH3-3). Each treatment had 5 replicates (one replicate per pot), with one pepper plant per pot (13.7 cm inner diameter), arranged randomly. Twelve days after the pepper seedlings recovered from transplanting (6-10 leaf stage), the bacterial solution treatment group was given 150 mL / pot of QGFCH3-3 bacterial solution diluted 300 times. The control group and the salt treatment group were given the same amount of water for root application. The bacterial solution treatment group and the salt treatment control group were simultaneously treated with 100 mmol / L NaCl solution for salt stress. The root application was repeated every 7 days for a total of 3 treatments. All treatment groups were managed in the same way (the base fertilizer was 1 g / pot of 17-17-17 water-soluble fertilizer). Samples were collected 18 days after the third treatment.

[0095] (4) Survey indicators and methods

[0096] The collected chili plants were investigated using the survey indicators and methods shown in Table 5.

[0097] Table 5. Survey Indicators and Methods for Chili Peppers

[0098]

[0099] (5) Test results

[0100] The experimental results are shown in Table 6 below.

[0101] Table 6 Results of QGFCH3-3 on salt tolerance and growth promotion in potted peppers

[0102]

[0103] Table 6 shows that all indicators of the CK group peppers were significantly lower than those of the CK0 group, indicating that salt stress significantly inhibited the growth of the entire pepper plant. In contrast, all indicators of the QH3-3 group peppers were improved to varying degrees compared to the CK group, suggesting that Bacillus amyloliquefaciens QGFCH3-3 has a certain promoting effect on pepper growth under salt stress.

Claims

1. Bacillus amyloliquefaciens QGFCH3-3, characterized in that, The Bacillus amyloliquefaciens QGFCH3-3 has a preservation number of CGMCC No. 34814.

2. The Bacillus amyloliquefaciens QGFCH3-3 according to claim 1, characterized in that, The Bacillus amyloliquefaciens QGFCH3-3 has at least one of the functions of nitrogen fixation, amylase production, ligninase production, protease production, and cellulase production.

3. A microbial agent comprising the Bacillus amyloliquefaciens QGFCH3-3 or a metabolite thereof according to claim 1.

4. The use of Bacillus amyloliquefaciens QGFCH3-3 according to claim 1 or the microbial agent according to claim 3 for controlling plant diseases caused by pathogenic organisms, characterized in that, The plant diseases include anthracnose, gray mold, wilt, sheath blight, scab, white star, and scab.

5. The use according to claim 4, wherein the compound is ###0002### The plant diseases include banana anthracnose, mango anthracnose, tomato gray mold, cotton wilt, rice sheath blight, wheat scab, banana wilt, pepper white star, and potato scab.

6. The use according to claim 4, wherein the compound is ###0002### The pathogens include Colletotrichum musae, Colletotrichum gloeosporioides penz, Colletotrichum asianum, Colletotrichum siamense, Colletotrichum fructicola, Botrytis cinerea, Fusarium oxysporum, Rhizoctonia solani, Fusarium graminearum, Fusarium asiaticum, Phyllosticta capsici, and Streptomyces.

7. The use of Bacillus amyloliquefaciens QGFCH3-3 of claim 1 or the bacterial agent of claim 3 in the field control of mango anthracnose, characterized in that, The field control method of the mango anthracnose comprises the following steps: at the early stage of the mango anthracnose, the microbial agent is sprayed on the mango plant, and the spraying is continuously performed for 3 times with an interval of 10-12 days.

8. Use according to claim 7, wherein the compound is ###0002### The microbial agent is a wettable powder of the Bacillus amyloliquefaciens QGFCH3-3, and the wettable powder comprises the following raw material components in mass percentage: 58.5% of the fermentation liquor of the Bacillus amyloliquefaciens QGFCH3-3, 30% of a carrier, 6.5% of a dispersing agent, 3.5% of a wetting agent, and 1.5% of a stabilizer; the carrier is prepared by mixing diatomite and kaolin at a mass ratio of 4:1, the dispersing agent is Tween-60, the wetting agent is sodium dodecyl sulfate, and the stabilizer is humic acid.

9. The Bacillus amyloliquefaciens QGFCH3-3 according to claim 1 or the microbial agent according to claim 3 is applied to promoting plant growth or promoting plant growth under salt stress.

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