Preparation method and application of high-activity solid-state fermented ganoderma lucidum compound for repairing hepatocyte function

By employing a three-stage compound fermentation system and specific post-processing techniques, the problem of low synthesis efficiency of Ganoderma lucidum polysaccharides and triterpenoids was solved, enabling the preparation of a highly active Ganoderma lucidum complex that repairs liver cell function. This complex can be applied to hepatoprotective drugs, functional foods, and hepatoprotective cosmetics, exhibiting significant bioactivity and economic benefits.

CN121695187APending Publication Date: 2026-03-20ZHONG KE YAO CHUANG (QING DAO) FA JIAO GONG CHENG YOU XIAN GONG SI
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Patent Information

Application Number
CN202511983739.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-26
Publication Date
2026-03-20

AI Technical Summary

Technical Problem

Existing solid-state fermentation technology for the synthesis of Ganoderma lucidum polysaccharides and triterpenoids suffers from insufficient synergistic enzymatic hydrolysis and complementary metabolism among multiple strains, resulting in low enhancement of active ingredients and limited synthesis efficiency, failing to meet the demand for high-purity and high-activity raw materials.

Method used

A three-stage compound fermentation system is adopted, including enzymatic pretreatment, probiotic-yeast synergistic prefermentation, and Ganoderma lucidum directional co-fermentation. Combined with a specific matrix formula and post-processing technology, the active ingredients are enriched through enzymatic degradation of macromolecules, synergistic action of probiotics and yeast, and directional fermentation of Ganoderma lucidum.

Benefits of technology

The product significantly increased the content of Ganoderma lucidum polysaccharides by ≥2.83 times and the content of triterpenoids by ≥1.55 times. In models such as acute liver injury and alcoholic liver injury, the product achieved a decrease of ALT and AST by ≥85%, and reduced the overall cost by about 42%, showing good prospects for industrialization.

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Abstract

The invention relates to the technical field of biological fermentation, in particular to a preparation method and application of a high-activity solid-state fermented ganoderma lucidum compound for repairing hepatocyte functions. According to the method, rice peptide, traditional Chinese medicines with homology of medicine and food and functional precursor substances are taken as substrates, and a three-stage composite process of enzymolysis pretreatment, synergistic pre-fermentation of probiotics and yeast and directional co-fermentation of lucid ganoderma is adopted. Through metabolism synergy and segmented temperature regulation and control of a specific strain, and in combination with an enzymolysis-water extraction-alcohol precipitation-ultrasonic composite post-treatment technology, oriented efficient enrichment of ganoderma lucidum polysaccharides and triterpenoids is realized. The content of active ingredients of the obtained compound is remarkably increased, and the obtained compound has excellent antioxidant, anti-inflammatory and hepatocyte repairing activity and can remarkably reduce ALT and AST levels in acute and chronic liver injury models. The method is good in process stability, low in production cost and suitable for development and production of liver protection drugs, functional foods and liver protection cosmetics.
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