Screening and identifying method of gene related to regulation and control of sperm motility of lion-head geese

By performing transcriptome sequencing and functional enrichment analysis on Lionhead goose testicular tissue, genes related to Lionhead goose sperm motility were screened and verified, solving the problems of missed detection and insufficient accuracy in gene screening in traditional methods, and achieving accurate gene screening and identification.

CN121780709APending Publication Date: 2026-04-03ZHONGKAI UNIV OF AGRI & ENG
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Patent Information

Application Number
CN202511983401.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-26
Publication Date
2026-04-03

AI Technical Summary

Technical Problem

Traditional methods for screening genes related to sperm motility in Lionhead geese typically employ single-gene validation or phenotypic association analysis, lacking transcriptome sequencing system detection, leading to missed detection of key regulatory genes and insufficient accuracy in identification.

Method used

Transcriptome sequencing was performed on testicular tissues of Lionhead geese with high and low sperm motility to obtain mRNA and non-coding RNA expression data. Differentially expressed nucleic acid molecules were screened, and functional enrichment analysis and quantitative real-time PCR were performed to identify candidate genes.

Benefits of technology

This method enables systematic screening and precise identification of genes related to sperm motility in Lionhead geese, improving the problem of missed detection in gene screening in traditional methods and enhancing the accuracy and efficiency of identification.

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Abstract

The invention relates to the technical field of lion-head goose breeding, in particular to a method for screening and identifying genes related to lion-head goose sperm motility regulation and control, which comprises the following steps: dividing healthy lion-head male geese in a breeding period into a high sperm motility group and a low sperm motility group according to sperm motility, collecting two groups of testicular tissues and sperm samples, and preserving the testicular tissues at low temperature for molecular detection; performing transcriptome sequencing on the testicular tissue to obtain mRNA and non-coding RNA expression data; screening two groups of nucleic acid molecules with significant differential expression, and determining candidate genes through functional enrichment analysis of reproductive development related biological pathways; and verifying the authenticity of the expression difference of the candidate gene through fluorescent quantitative PCR, and finally determining the target gene. According to the method, related data are obtained through sequencing, the candidate genes are determined by screening differential molecules through enrichment analysis and PCR verification, accurate identification of a related gene system is achieved, and the problems of missing detection and insufficient accuracy caused by lack of transcriptome sequencing in a traditional method are solved.
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