Fluorescent RNA functionalized hydrogel kit, preparation method and method for detecting colorectal cancer miRNA-126

By combining a fluorescent RNA functionalized hydrogel kit with a fluorescence microscope, the challenge of non-invasive detection of miRNA-126 has been solved, enabling rapid and accurate detection for early screening of colorectal cancer.

CN121978325APending Publication Date: 2026-05-05WENZHOU KANGRUI BAIOU BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202610012227.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-01-06
Publication Date
2026-05-05

AI Technical Summary

Technical Problem

Existing technologies are not effective and non-invasive in detecting miRNA-126 in blood or tissue samples, leading to poor patient compliance in colorectal cancer screening.

Method used

A hydrogel kit for functionalizing fluorescent RNA was used to achieve qualitative and quantitative analysis by specifically binding the fluorescent RNA aptamer to miRNA-126 and then detecting it using fluorescence microscopy.

Benefits of technology

It enables rapid and accurate detection of miRNA-126, with a high signal-to-noise ratio and strong anti-interference capabilities, making it suitable for point-of-care testing and early screening for colorectal cancer.

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Abstract

The invention relates to a fluorescent RNA (Ribonucleic Acid) functionalized hydrogel kit, a preparation method and a method for detecting colorectal cancer miRNA-126, solves the technical problem of how to detect miRNA-126 in blood or tissue samples and is suitable for portable detection, and fluorescent RNA functionalized hydrogel is prepared from methacrylic acid hyaluronic acid, an acryloyl-modified fluorescent RNA aptamer and N, N-dimethylformamide. The fluorescent RNA aptamer is obtained by crosslinking the fluorescent RNA aptamer and N, N '-methylene bisacrylamide, and the covalent crosslinking between the fluorescent RNA aptamer and the hydrogel can prevent the RNA aptamer from being released into the external environment, so that the stability and the anti-interference capability of the hydrogel kit are improved.
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