A sex pheromone of the rice leaf roller ester biotype and its preparation and application as a lure.

Abstract

This invention discloses a sex pheromone of the rice leaf folder (RLM) ester-based biotype, its preparation and application as a lure, and the pheromone comprises a mixture mainly composed of acetates, with the main components being cis-11-hexadecene acetate (Z11-16:Ac), cis-11-octadecene acetate (Z11-18:Ac), and cis-13-octadecene acetate (Z13-18:Ac). The novel RLM pheromone proposed in this invention plays an important role in the RLM population, and its distribution is influenced by geographical and seasonal factors. This discovery confirms the significant geographical population differentiation of the rice leaf folder and provides important guidance for optimizing the selection and application of sex pheromone lures in regional monitoring and early warning, improving forecast accuracy, and enhancing the effectiveness of swarm trapping and control.

Description

Technical Field

[0001] This invention belongs to agricultural biotechnology, and particularly relates to a sex pheromone of the rice leaf roller ester biotype and its preparation and application as a lure. Background Technology

[0002] The rice leaf roller (Cnaphalocrocis medinalis Guenee) (Lepidoptera, Pyralidae) is an important long-distance migratory pest in rice-growing areas of China, excluding Northeast and North China. The severity and timing of its occurrence vary significantly across different years and rice-growing regions, exhibiting distinct intermittent and sudden outbreaks. The rice leaf roller shows a relatively weak response to light; therefore, using light traps to monitor its field population dynamics is not ideal. Furthermore, urban light sources often further weaken the effectiveness of light trapping. Given these issues, the moth-driving method is widely used in China for monitoring this pest population. However, this method is labor-intensive, has low data accuracy, and struggles to differentiate between species when multiple pests coexist in rice fields. In contrast, pheromone technology is a more suitable alternative monitoring method. As a migratory pest, the rice leaf roller exhibits different physiological states, such as ovarian development level and mating frequency, which can lead to its classification into immigrant generations, partially immigrant generations, locally breeding generations, and emigrant and partially emigrant generations. Different migration states lead to significant differences in their responses to odor compounds. The origin of the insects varies greatly by region and is influenced by climatic conditions.

[0003] The sex pheromone composition of the rice leaf roller exhibits significant regional differences. For example, Indian and Philippine populations used (Z)-11-hexadecenyl acetate (Z11-16:Ac) and (Z)-13-octadecenyl acetate (Z13-18:Ac), respectively, in a mixture ratio of 1-6:20 (India) and 98:2 (Philippines), and (Z)-13-18:OH was added to these components. This will reduce the capture rate of the traps. In Japan and China, the sex pheromones were identified as a mixture of four compounds: (Z)-11-octadecenal, (Z)-13-octadecenal, (Z)-11-octadecenol, and (Z)-13-octadecenol, in a ratio of 55µg:500µg:120µg:180µg. Two of the aldehydes were essential for trapping, while the alcohols had a synergistic effect, although their proportions varied slightly. Trapping trials conducted in China and Vietnam found that the pheromone mixture effective against the rice leaf roller in the Philippines and India was ineffective in China, while the Japanese mixture showed only moderate trapping effectiveness. Similar conclusions were reached in Indonesia.

[0004] Because the rice leaf roller is a migratory pest, it may be brought by the southwest monsoon from Myanmar, Thailand, Laos, and Vietnam, or by the southeast monsoon from Indonesia and the Philippines. In the same area, rice leaf roller populations from different sources may converge. Since they belong to the same species, individuals from these different sources are likely to hybridize. However, even in the same paddy field, there may be rice leaf rollers from different sources, and their sex pheromones may differ. If only one sex pheromone formula is used, only some male moths will be captured, while the rest will not be captured due to the mismatch in the sex pheromone formula. The applicant has frequently encountered this problem in years of field monitoring, making it difficult for sex-trapping data to accurately reflect the actual population dynamics in the field, or causing some deviation from data from moth removal and light trapping. Therefore, exploring whether other types of sex pheromones exist in the field for the rice leaf roller, thereby improving the overall monitoring efficiency of the rice leaf roller, is of great significance. Summary of the Invention

[0005] Purpose of the invention: In view of the defects and deficiencies of the existing technology, the present invention provides a novel sex pheromone of rice leaf roller ester ester biotype, which effectively solves the problem that the current sex pheromone monitoring and control is not comprehensive enough, and only a portion of the male moths in the field are captured, or even that male moths cannot be captured at all in some areas.

[0006] The present invention also provides the preparation and application of the lure core based on the sex pheromone of the rice leaf roller.

[0007] Technical solution: In order to achieve the above objectives, the present invention provides a sex pheromone of the rice leaf roller ester biotype, comprising a mixture mainly composed of acetate, wherein the main components are cis-11-hexadecene acetate (Z11-16:Ac), cis-11-octadecene acetate (Z11-18:Ac) and cis-13-octadecene acetate (Z13-18:Ac).

[0008] The mass ratio of cis-11-hexadecene acetate (Z11-16:Ac), cis-11-octadecene acetate (Z11-18:Ac), and cis-13-octadecene acetate (Z13-18:Ac) in the sex pheromone type is 100-1000:10-100:5-50.

[0009] Preferably, the ratio of cis-11-hexadecene acetate (Z11-16:Ac), cis-11-octadecene acetate (Z11-18:Ac), and cis-13-octadecene acetate (Z13-18:Ac) in the sex pheromone type is 504:27:23.

[0010] Among them, Z11-16:Ac is the dominant sex pheromone type, with an individual content of 5.04±0.72 ng / individual.

[0011] The sex pheromone type is only present in female moths, and the ovarian maturity of female moths is level 2-4, with mating times ranging from 0 to 5.

[0012] The sex pheromone lure for the rice leaf roller of the present invention comprises sex pheromone compounds Z11-16:Ac (1008µg), Z11-18:Ac (54µg) and Z13-18:Ac (23µg), 1% antioxidant (BHT), and a slow-release material PVC capillary.

[0013] The preparation method of the lure core of the present invention includes the following steps: mixing and dissolving three compounds, cis-11-hexadecene acetate (Z11-16:Ac), cis-11-octadecene acetate (Z11-18:Ac), and cis-13-octadecene acetate (Z13-18:Ac), in a solvent, then mixing with an antioxidant, and adding to a slow-release material to form the lure core.

[0014] Further, the three compounds Z11-16:Ac, Z11-18:Ac, and Z13-18:Ac are mixed and dissolved in petroleum ether, then mixed with 1% antioxidant (BHT), added to a PVC capillary tube, and sealed to form an induction core.

[0015] The application of the rice leaf folder acetate biotype sex pheromone described in this invention in the monitoring and control of rice leaf folder in the field.

[0016] The application of the lure described in this invention in the trapping of rice leaf rollers.

[0017] The application of the rice leaf folder acetate biotype sex pheromone, along with other aldehyde and / or alcohol-type sex pheromones, described in this invention, in field monitoring and control of rice leaf folder populations.

[0018] Among them, the other types of sex pheromones are CM type, including Z13-18:Ald, Z11-18:Ald, Z13-18:OH and Z11-18:OH.

[0019] This invention, through pheromone analysis of rice leaf folder from multiple insect source areas in China and extensive field trapping experiments, confirms the existence of two types of sex pheromones in the rice leaf folder. The newly discovered type differs from those found in previous literature: one is a mixture of traditionally known aldehydes and alcohols (CM, including Z13-18:Ald, Z11-18:Ald, Z13-18:OH, and Z11-18:OH), and the other is a newly discovered mixture primarily composed of acetates (RLM, mainly composed of Z11-16:Ac, Z11-18:Ac, and Z13-18:Ac). The two types differ significantly in their chemical composition. In terms of individual content, the CM type has a higher Z13-18:Ald content (4.30±2.68 ng / individual), while the RLM type is dominated by Z11-16:Ac (5.04±0.72 ng / individual). Field surveys show that the RLM type is absolutely dominant in female moths. Using both larval emergence and adult trapping methods, identification at multiple locations (such as Jiangsu, Zhejiang, and Guangdong) revealed that the proportion of RLM (rice leaf roller) was significantly higher than that of CM (rice leaf roller). Furthermore, the ovarian grading of female moths was mostly between 2 and 4, and the number of matings ranged from 0 to 5, indicating that the RLM type is more prevalent in natural populations. A large-scale field trial at 106 monitoring points in the perennial occurrence areas of the rice leaf roller in China revealed the geographical distribution patterns of the two types. The number of moths attracted by CM and RLM showed significant regional and seasonal differences. In southern China (such as Guangdong, Guangxi, and southern Hunan), the proportion of RLM was higher in July and August, while in other regions (especially the provinces in the middle and lower reaches of the Yangtze River), CM dominated in August and September, often exceeding 60%-80%. For example, the proportion of CM at Nantong, Jiangsu, reached over 90%, while the proportion of RLM at Huaijing, Hunan, remained consistently above 80%. In some southern locations (such as Chongzuo, Guangxi), RLM surpassed CM in September. In summary, the novel type of rice leaf folder sex pheromone (RLM) proposed in this invention plays an important role in the rice leaf folder population, and its distribution is influenced by geographical and seasonal factors. This discovery confirms the existence of significant geographical population differentiation in the rice leaf folder, and has important guiding significance for optimizing the selection and application of sex pheromone decoys in regional monitoring and early warning, and improving the accuracy of forecasting.

[0020] Beneficial effects: Compared with the prior art, the present invention has the following significant advantages:

[0021] This invention provides a novel sex pheromone for the rice leaf folder biotype. This pheromone differs significantly from existing sex pheromones in its chemical composition. The novel type of acetate-based sex pheromone for the rice leaf folder proposed in this invention effectively solves the problem that current sex pheromone monitoring and control is not comprehensive enough, only a portion of male moths are captured in the field, and in some areas, male moths are not captured at all. This improves the overall monitoring efficiency and the effect of cluster trapping and control of the rice leaf folder. The lure prepared from this pheromone can be effectively used for trapping and killing rice leaf folders in the field.

[0022] The novel rice leaf roller acetate biotype sex pheromone provided by this invention can be used in combination with existing aldehyde and alcohol combination sex pheromones. The lure prepared by the two can ensure the capture of all male rice leaf roller moths in the field, thus ensuring more comprehensive monitoring and control of the rice leaf roller population. Attached Figure Description

[0023] Figure 1 Chromatograms of sex components for different types of rice leaf rollers. Detailed Implementation

[0024] The present invention will be further described below with reference to the embodiments.

[0025] Unless otherwise specified, the experimental methods described in the examples are conventional methods; the materials and reagents used in the examples are commercially available unless otherwise specified.

[0026] Example 1

[0027] 1. Materials and Methods

[0028] 1.1 Collection from insect source sites

[0029] The insects used in the experiment were obtained through two methods: direct net capture and laboratory rearing.

[0030] Female moths were captured by netting: In rice-producing areas, standard insect-catching nets were used to sweep the rice canopy at a uniform speed. The captured female moths were placed in ventilated net cages, and any captured natural enemies such as spiders were promptly removed. Young rice seedlings were placed in the cages to provide habitat for the moths. Sterile water was sprayed onto the cage walls regularly to maintain humidity. After being brought back to the laboratory, the moths were placed in an incubator (temperature 25±1°C, relative humidity 75%±5%, photoperiod L:D = 14h:10h) for later use.

[0031] Laboratory rearing: Rolled leaves damaged by the rice leaf roller were collected from the field and brought back to the laboratory. They were placed in an incubator, and untreated rice seedlings were placed in the insect rearing cages for rearing. After the larvae pupated, they were transferred to adult rearing cages (under the same conditions as when netting female moths). Within 24 hours of emergence, male and female moths were separated and fed with a 10% (v / v) glucose solution to obtain unmated female moths with consistent physiological states, which were then used as backup experimental materials.

[0032] 1.2 Extraction and Analysis

[0033] Female moths were captured in the field and brought back to the laboratory for extraction. Sex pheromones were extracted from 3-day-old female moths after emergence, starting 3 hours after darkness. The abdomen of the female moth was gently squeezed to expose the gonads. SPME adsorption was used according to the method of Hughes and Cardé (2020) (Hughes GP & Carde RT (2020) Do Helicoverpa armigera MothsSignal Their Fecundity by Emission of an Antagonist Journal of Chemical Ecology 46: 21-29.), using PDMS / DVB coated fibers (100 μm membrane thickness, Supelco Inc.) to directly adsorb secretions from the glandular surface. Sample analysis was performed using an Agilent 6890GC-5975MS gas chromatography-mass spectrometry system equipped with a DB-FastFAME capillary column (60 m × 0.25 mm × 0.25 μm). The temperature program was set as follows: initial column temperature 60℃, held for 1 minute; then increased to 180℃ at 10℃ / min, followed by an increase to 210℃ at 3℃ / min, and finally increased to 250℃ at 10℃ / min and held for 10 minutes. The ion source temperature was 230℃, the electron bombardment energy was 70 eV, and the carrier gas was high-purity helium. For quantitative analysis of pheromone titers, the external standard method was used in this experiment.

[0034] First, standard solutions of target compounds such as (Z)-11-hexadecenal (the main component) at different concentrations were prepared using chromatographically pure standards, and a standard curve was established. Samples and standards were analyzed under the same instrument conditions. By comparing the peak areas of the samples with the standard curve, the absolute content of each component of the sex pheromone in each female moth was calculated, and the results are expressed in nanograms (ng) per moth.

[0035] 1.3 Anatomy and Grading

[0036] Pour the prepared 50% alcohol into a petri dish, and place the removed female abdomen in it before dissection. Gently tear open the abdominal epidermis with ophthalmic forceps to expose the ovary, and then remove the fat body and other irrelevant tissues attached to the ovary. Observe the morphology, size and other characteristics of the eggs under a dissecting microscope, and determine the ovarian development level according to the five-level classification standard proposed by Zhang Xiaoxi et al. (1979) (Zhang Xiaoxi, Lu Ziqiang, Geng Jiguo, 1979. Application of dissection of female rice leaf roller moth in forecasting. Entomology Knowledge, 16(3): 97-99), and take pictures and record them. In addition, the number of residual spermatophores in the female insect body is counted as an indicator of the number of matings of the individual.

[0037] 1.4 Field Trapping Experiment

[0038] Preparation of sex pheromone lure core: Sex pheromone compounds Z11-16:Ac (1008µg), Z11-18:Ac (54µg) and Z13-18:Ac (23µg) were mixed and dissolved in 16μL of petroleum ether or hexane. Then, 1% antioxidant (BHT) (10µg) was added, and the mixture was poured into a PVC capillary tube (10 cm long, 1.6 mm outer diameter, 0.8 mm inner diameter) and sealed to form the lure core.

[0039] Meanwhile, lures prepared using aldehyde and alcohol-type sex pheromones (CM) were used as a comparison.

[0040] Each trap contained one sex pheromone lure. During the rice seedling stage, the trap's bottom was positioned approximately 0.8 m above the ground. Once the rice had grown taller, the height was adjusted so the bottom was 10–20 cm below the rice leaves. Experimental plots were selected from areas with similar growth and consistent cultivation management. The lures were loaded into the assembled traps, marked, and then inserted into the paddy fields. The spacing between adjacent traps was 30 meters. Each treatment was replicated six times, with the traps arranged in a rotating order across replicates to minimize interference from location and wind direction. The number of rice leaf rollers captured in the traps was collected and recorded each morning. Multiple locations were established in Guangdong, Guangxi, Hunan, and Jiangsu provinces, where traps were placed during the rice leaf roller emergence period from July to September, and the number of moths captured using both types of lures was recorded.

[0041] 1.5 Statistical Analysis

[0042] Data analysis was performed using SPSS (SPSS 2008). One-way ANOVA was conducted on the trap capture data. The means of the two trap captures were compared and distinguished using Duncan's multiple range test (P < 0.05).

[0043] 2. Results and Analysis

[0044] 2.1 Two types of sex pheromones and their mixed populations

[0045] This invention confirms that there are two types of sex pheromones in the field of rice leaf roller. Besides the commonly used aldehyde and alcohol type (CM), a mixture of Z13-18:Ald, Z11-18:Ald, Z13-18:OH, and Z11-18:OH, a novel type of acetate sex pheromone (RLM) has been discovered. Its main components are a mixture of Z11-16:Ac, Z11-18:Ac, and Z13-18:Ac. Figure 1 ).

[0046] The abundance of Z13-18:Ald fragment ions is as follows: 55 (100%), 67 (47%), 69 (66%), 83 (57%), 95 (57%), 111 (32%), 121 (29%), 149 (10%), 248 (19%), 266 (9%).

[0047] The abundances of Z9-16:AC fragment ions were 55 (60%), 61 (7%), 67 (66%), 82 (100%), 96 (92%), 110 (44%), 124 (32%), 194 (6%), and 222 (32%), respectively; the abundances of Z11-16:AC fragment ions were 55 (71%), 61 (10%), 67 (56%), 82 (100%), 96 (91%), 110 (38%), 124 (2%), 194 (3%), and 222 (31%), respectively; and the abundances of Z11-18:Ac fragment ions were 55 (56%), 67 (50%), 82 (100%), 96 (100%), 110 (41%), and 124 (32%), respectively. The abundances of Z13-18:Ac fragment ions were as follows: 138 (21%), 166 (6%), 222 (5%), 250 (35%); 55 (65%), 67 (49%), 69 (40%), 82 (98%), 96 (100%), 110 (41%), 124 (30%), 138 (20%), 166 (7%), 222 (5%), 250 (37%).

[0048] In the extract of rice leaf folder (CM), the content of cis-13-octadecenal (Z13-18:Ald) was generally high, with a content of 4.30±2.68 ng / individual. However, the content of cis-11-octadecenal (Z11-18:Ald), cis-11-octadecenol (Z11-18:OH), and cis-13-octadecenol (Z13-18:OH) was not significant. Cis-11-hexadecene acetate (Z11-16:Ac), cis-11-octadecene acetate (Z11-18:Ac), and cis-13-octadecene acetate (Z13-18:Ac) were not detected.

[0049] In the RLM type, the main component was cis-11-hexadecene acetate (Z11-16:Ac), with a relatively high content of 5.04±0.72 ng / individual. Trace amounts of cis-11-octadecene acetate (Z11-18:Ac) and cis-13-octadecene acetate (Z11-18:Ac) were also detected, at contents of 0.27±0.07 ng / individual and 0.23±0.08 ng / individual, respectively. Cis-13-octadecene aldehyde (Z13-18:Ald) was not detected.

[0050] The specific results are shown in Table 1:

[0051] Table 1. Content of sex pheromone components in different types of rice leaf rollers

[0052]

[0053] 2.2 Field distribution and development status

[0054] Two methods were used to extract and identify the sex pheromones of the rice leaf roller: 1) adult moths were obtained from pupae raised in the field and identified after sexual maturity; 2) adult moths were directly captured in the field by netting, and the pheromones were extracted and identified on the same evening. The results are shown in Table 2. In the identification of female adults after larval emergence, no CM pheromones were identified in female moths from Jurong, Jiangsu, but 80% showed RLM pheromones, with ovarian levels at grades 2 and 3. No CM or RLM pheromones were identified in female moths from Dongyang, Zhejiang, but all dissected ovaries were at grade 3. CM pheromones were identified in female moths from Huazhou, Guangdong, but 80% showed RLM pheromones, with ovarian levels at grades 2 and 3. In the identification of female adults captured by netting in the field, 56% of female moths from Nanning, Guangxi, showed R pheromones, with dissected ovaries at grades 4 and 5, and mating frequency ranging from 1 to 4 times. In Xishuangbanna, Yunnan, 63% of female moths were identified as having RLM sex pheromones, with all ovaries classified as grade 4 and having mated only once. In Leizhou, Guangdong, 88% of female moths were identified as having RLM sex pheromones, with 4% being CM and 96% being RLM sex pheromones, ovaries classified as grades 3 and 4, and mating occurring 0-2 times. In Wuxi, Jiangsu, 19% of female moths were identified as having RLM sex pheromones, with 11% being CM and 89% being RLM sex pheromones, ovaries classified as grades 3 and 4, and mating occurring 0-1 times. In Dongyang, Zhejiang, 26% of female moths were identified as having RLM sex pheromones, with 17% being CM and 83% being RLM sex pheromones, ovaries classified as grades 1, 2, 3, and 4, and none having mated. In Haishu, Zhejiang, 57% of female moths were identified as having RLM sex pheromones, with 3% being CM and 97% being RLM sex pheromones, ovaries classified as grades 3 and 4, and mating occurring 0-1 times.

[0055] These results show that the percentage of RLM sex pheromones is significantly higher than that of CM sex pheromones. Furthermore, female rice leaf rollers in the field can mate up to five times, further demonstrating that RLM sex pheromones result in more mating attempts, indicating that trapping is more effective in controlling the pest.

[0056] Table 2. Identification of sex pheromones and developmental status of female rice leaf rollers captured by nets.

[0057]

[0058] 2.3 Results of the implementation of two types of sex pheromones for field trapping

[0059] Table 3 shows that the overall moth catch using CM lures exhibited a significant upward trend from July to September. Many monitoring sites in the south were able to capture male moths as early as July, while the peak trapping period in the north was generally concentrated in August and September. In southern regions (such as Guangdong, Guangxi, southern Hunan, and Guizhou), many monitoring sites showed a dominant proportion of moths caught using RLM lures in July and August, and even throughout the entire monitoring period. In northern regions (such as the provinces along the middle and lower reaches of the Yangtze River), CM lures generally dominated the population proportion. Especially in September, the proportion of CM lures exceeded 60% at the vast majority of sites, and in many cases even exceeded 80%.

[0060] In July, the trapping counts at the monitoring point in Tongzhou District, Nantong City, Jiangsu Province, were CM (8.33 individuals, 89.7%) and RLM (1.00 individuals, 10.3%). CM had become the dominant species, but the overall number was low, possibly due to initial migration. In August, the trapping counts gradually increased: CM (96.33 individuals, 91.2%) and RLM (9.33 individuals, 8.8%). The CM count surged more than tenfold, and its proportion further expanded. In September, the CM counts were 275.33 individuals (92.9%) vs. RLM (21.00 individuals, 7.1%). The CM count reached its peak.

[0061] At the monitoring site in Jingzhou County, Huaihua City, Hunan Province, the number of moths trapped were as follows: July: CM (1.67, 17.0%), RLM (8.17, 83.0%); August: CM (3.67, 15.3%), RLM (20.33, 84.7%); September: CM (1.00, 8.8%), RLM (10.33, 91.2%). Throughout the monitoring period, the proportion of RLM at this site consistently exceeded 80%, while the proportion of CM was very low and the number of moths remained consistently small.

[0062] The proportion of sex pheromones used in some monitoring sites showed a reversal. For example, in Tiandeng County, Chongzuo City, Guangxi Province, the proportion of CM (Chemical Moth) traps was higher than that of RLM (Relative Moth) traps in July and August. However, in September, the proportion reversed dramatically, with RLM surpassing CM in both the number of moths and the percentage of moths trapped. The above experiments effectively demonstrate that the new biotype of rice leaf folder sex pheromone proposed in this invention, when prepared as a lure, can trap some or all of the male moths in rice fields that cannot be trapped by the old lures (prepared using the CM type). The novel rice leaf folder biotype sex pheromone RLM provided by this invention can be used in combination with the existing sex pheromone CM. The lures prepared by both ensure more comprehensive monitoring and control of rice leaf folder populations.

[0063] Table 3 Comparison of the effectiveness (number of insects attracted) of the new (RLM) and old (CM) lures.

[0064]

[0065]

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[0068]

Claims

1. A sex pheromone of the rice leaf roller ester biotype, characterized in that, It includes a mixture mainly composed of acetates, with the main components being cis-11-hexadecene acetate (Z11-16:Ac), cis-11-octadecene acetate (Z11-18:Ac) and cis-13-octadecene acetate (Z13-18:Ac).

2. The sex pheromone of the rice leaf roller acetate biotype according to claim 1, characterized in that, The mass ratio of cis-11-hexadecene acetate (Z11-16:Ac), cis-11-octadecene acetate (Z11-18:Ac), and cis-13-octadecene acetate (Z13-18:Ac) in the sex pheromone type is 100-1000:10-100:5-50.

3. The sex pheromone of the rice leaf roller acetate biotype according to claim 1, characterized in that, The sex pheromone type Z11-16:Ac is dominant, with an individual content of 5.04±0.72 ng / individual.

4. The sex pheromone of the rice leaf roller biotype according to claim 1, characterized in that, The sex pheromone type is only present in female moths, and the ovaries of female moths are at maturity level 2-4, with mating times ranging from 0 to 5.

5. A lure core for a sex pheromone of the rice leaf roller ester biotype, characterized in that, The lure core includes the sex pheromone of the rice leaf roller as described in claim 1.

6. A method for preparing the lure core according to claim 5, characterized in that, The process includes the following steps: mixing and dissolving three compounds, cis-11-hexadecene acetate (Z11-16:Ac), cis-11-octadecene acetate (Z11-18:Ac), and cis-13-octadecene acetate (Z13-18:Ac), in a solvent, then mixing them with an antioxidant, and finally adding them to a sustained-release material to form a core.

7. The application of the sex pheromone of the rice leaf folder acetate biotype as described in claim 1 in field monitoring and control of rice leaf folder populations.

8. The application of the lure as described in claim 5 in the trapping and killing of rice leaf rollers in the field.

9. The application of the rice leaf folder acetate biotype sex pheromone as described in claim 1, in combination with other aldehyde and / or alcohol-type sex pheromones, in field monitoring and control of rice leaf folder populations.

10. The application according to claim 9, characterized in that, The other aldehyde and / or alcohol types of sex pheromones include Z13-18:Ald, Z11-18:Ald, Z13-18:OH, and Z11-18:OH.

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