A whitening and anti-aging composition containing birch sap and application thereof

Abstract

This invention relates to the field of cosmetic technology, and more particularly to a whitening and anti-aging composition containing birch sap and its application. The whitening and anti-aging composition containing birch sap of this invention comprises the following components in parts by weight: 0.5-15 parts of Asian birch sap, 1-4 parts of kava root extract, and 1-4 parts of mung bean seed extract. This invention, by combining Asian birch sap, kava root extract, and mung bean seed extract, achieves a synergistic effect. When applied to cosmetics, volunteers who used it experienced rapid results, with significant anti-aging, brightening, and whitening effects, resulting in smooth and delicate skin.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a whitening and anti-aging composition containing birch sap and its application. Background Technology

[0002] With the advancement of technology, people are accustomed to using electronic products. Electronic light can damage the skin. Combined with severe environmental pollution, excessive ultraviolet radiation, improper skin cleansing, endocrine disorders, aging, and disease, these factors lead to darkened and dull skin, affecting the normal function and metabolism of skin cells. This results in melanin deposition or changes in skin condition, ultimately causing dull and lackluster skin. Specific manifestations of dull and lackluster skin include: Pigmentation: Ultraviolet radiation stimulates melanin production, leading to age spots and uneven skin tone in the epidermis; Collagen loss: Collagen fibers in the dermis break down, reducing skin elasticity and deepening fine lines and nasolabial folds; Barrier damage: The stratum corneum's ability to retain moisture weakens, resulting in dry, rough skin and accelerated aging.

[0003] To address dull and lackluster skin, whitening and anti-aging treatments are necessary. The core of whitening and anti-aging lies in reducing melanin production, promoting collagen regeneration, and improving skin texture to achieve a more even skin tone and firmer complexion. However, existing whitening and anti-aging cosmetics suffer from slow results (requiring continuous use for at least 15 days), short-lasting whitening effects, and low safety, often leading to skin allergies. Therefore, there is an urgent need for whitening and anti-aging products that are safe and fast-acting. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the existing technology and provide a whitening and anti-aging composition.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a whitening and anti-aging composition comprising the following components in parts by weight: 0.5-15 parts of Asian birch sap, 1-4 parts of kava root extract, and 1-4 parts of mung bean seed extract.

[0006] Traditional whitening and anti-aging products are slow to show results, requiring continuous use for more than 15 days. This invention, through extensive research, has discovered that combining Asian birch sap, kava root extract, and mung bean seed extract can synergistically enhance their effects. When applied to cosmetics, volunteers who used the product experienced rapid results, with significant anti-aging, brightening, and whitening effects, leaving the skin smooth and delicate.

[0007] Birch sap contains betulin and betulinic acid, among other betulinic triterpenoids, and is rich in 17 amino acids, 8 trace elements, polyphenols, and flavonoids. The main active ingredients of kava pepper are kavalactones, capsaicin, and capsaicin, which have sedative effects. Flavonoids and phytosterols in mung bean seed extract also have some antibacterial and antiviral effects. This invention combines Asian birch sap, kava pepper root extract, and mung bean seed extract; the three work synergistically to promote skin metabolism, reduce melanin deposition, and resist external irritants, thereby achieving anti-aging, brightening, and whitening effects.

[0008] Preferably, the whitening and anti-aging composition comprises the following components in parts by weight: 8-15 parts of Asian birch sap, 1-2 parts of kava root extract, and 1-2 parts of mung bean seed extract.

[0009] More preferably, the whitening and anti-aging composition comprises the following components in parts by weight: 8 parts of Asian birch sap, 1 part of kava root extract, and 1 part of mung bean seed extract.

[0010] Secondly, the present invention provides the application of the whitening and anti-aging composition in the preparation of whitening and anti-aging cosmetics.

[0011] Thirdly, the present invention provides a whitening and anti-aging cosmetic, comprising the above-mentioned whitening and anti-aging composition.

[0012] Preferably, it also includes cosmetic excipients.

[0013] Preferably, the amount of the whitening and anti-aging composition added is 8.5%-19% of the total mass of the whitening and anti-aging cosmetic.

[0014] Preferably, the excipients include at least one of skin conditioning agents, emollients, humectants, preservatives, fragrances, emulsifiers, emulsifying aids, whitening agents, and water.

[0015] Preferably, the skin conditioning agent includes at least one of hydroxyproline, conchiolin powder, and apple (Pyrus Malus) fiber.

[0016] Preferably, the emollient comprises at least one of isododecane, glyceryl tri(ethylhexanoate), and isononyl isononanoate.

[0017] Preferably, the moisturizer includes at least one of glycerin, butylene glycol, and 1,2-hexanediol.

[0018] Preferably, the preservative includes phenoxyethanol.

[0019] Preferably, the fragrance agent includes a flavoring.

[0020] Preferably, the emulsifier includes at least one of glyceryl stearate and PEG-100 stearate.

[0021] Preferably, the emulsifying agent comprises cetearyl alcohol.

[0022] Preferably, the whitening agent comprises 3-o-ethyl ascorbic acid.

[0023] Preferably, the whitening and anti-aging cosmetic comprises the following components by weight percentage: 8.5%-19% whitening and anti-aging composition, 0.06-0.25% skin conditioning agent, 4-17% emollient, 8-26% moisturizer, 0.3-1% preservative, 0.05-0.2% fragrance, 1.8-8% emulsifier, 1-3% emulsification aid, 0.5-10% whitening agent, and water to make up to 100%.

[0024] Preferably, the skin conditioning agent is a compound of hydroxyproline, conchiolin powder, and apple (Pyrus Malus) fiber.

[0025] Preferably, the emollient is a compound of isododecane, glyceryl tri(ethylhexanoate) ester and isononyl isononanoate.

[0026] Preferably, the humectant is a compound of glycerin, butylene glycol and 1,2-hexanediol.

[0027] Preferably, the preservative is phenoxyethanol.

[0028] Preferably, the fragrance is a flavoring.

[0029] Preferably, the emulsifier is a compound of glyceryl stearate and PEG-100 stearate.

[0030] Preferably, the emulsifying agent is cetearyl alcohol.

[0031] Preferably, the whitening agent is 3-o-ethyl ascorbic acid.

[0032] Preferably, the dosage form of the cosmetic includes water, lotion, and cream.

[0033] The beneficial effects of this invention are as follows: This invention combines Asian birch sap, kava root extract, and mung bean seed extract, which can synergistically enhance the effects. When applied to cosmetics, volunteers who used it experienced rapid results, with significant anti-aging, brightening, and whitening effects, resulting in smooth and delicate skin.

[0034] The whitening and anti-aging product provided by this invention has good fluidity, moisturizing properties, and a light texture. It adheres well to the face, blends seamlessly with the skin, and provides a pleasant user experience. It has whitening, spot-removing, anti-aging, and brightening effects. Volunteers showed a noticeable brightening and whitening effect on their skin after 5 days of use. Attached Figure Description

[0035] Figure 1 The results show the inhibition rate of elastase activity by the sample in Example 1.

[0036] Figure 2 The results show the inhibition rate of elastase activity by the sample in Example 2.

[0037] Figure 3 The results show the inhibition rate of elastase activity of the sample in Example 3.

[0038] Figure 4 The results show the inhibition rate of tyrosinase activity by the sample in Example 1.

[0039] Figure 5 The results show the inhibition rate of tyrosinase activity by the sample in Example 3. Detailed Implementation

[0040] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0041] Unless otherwise specified, the experimental methods used in this invention are conventional methods, and the materials and reagents used are commercially available products.

[0042] The sap of the Asian birch (BETULA PLATYPHYLLA) of this invention was purchased from Guangzhou Liyan Biotechnology Co., Ltd.

[0043] Kava Pepper Root Extract: Kava pepper (PIPER METHYSTICUM) root extract, purchased from Guangzhou Kelide Daily Chemical Technology Co., Ltd.

[0044] Mung bean seed extract: Mung bean (PHASEOLUS RADIATUS) seed extract, purchased from Guangzhou Kelide Daily Chemical Technology Co., Ltd.

[0045] The product prepared by this invention has the following appearance: a flowing emulsion state.

[0046] Heat resistance (45±2℃): 2 months, returned to room temperature, no abnormalities observed; Cold resistance (-18±2℃): After 2 months, no abnormalities were observed after returning to room temperature; Cyclic test (45℃, room temperature, -18℃): 3 cycles, passed.

[0047] Examples 1-4: Examples 1-4 respectively provide the whitening and anti-aging compositions of the present invention, the components and weight proportions of which are shown in Table 1.

[0048] Table 1 (Unit: parts by weight) Comparative Examples 1-2: Comparative Examples 1 and 2 each provide a whitening and anti-aging composition, the components and weight proportions of which are shown in Table 2.

[0049] Table 2 (Unit: parts by weight) Application Examples 1-4: Application Examples 1-4 each provide a whitening and anti-aging cosmetic, specifically a whitening and anti-aging cream. Application Examples 1-4 each include the whitening and anti-aging compositions described in Examples 1-4, and the specific formulas are shown in Table 3 (total weight parts are 100 parts).

[0050] Table 3 (Unit: parts by weight) The preparation method of the whitening and anti-aging cosmetic includes the following steps: 1. Mix the raw materials of phase A and heat them to 80-85℃, then stir them at 3000 rpm to homogenize and disperse them evenly until they are transparent and free of bubbles to obtain mixture A; 2. Add raw material B to mixture A sequentially, stir well, and keep warm at 80-85℃ for 30 minutes to obtain mixture B; 3. Cool down to 60℃, then slowly add the C phase raw material to mixture B and stir until homogeneous to obtain mixture C; 4. Cool to below 45℃, then add phase D raw material sequentially to mixture C, and stir for 30 minutes. Take a sample for confirmation; if qualified, discharge the material to obtain the whitening and anti-aging cosmetic.

[0051] Application Comparative Example 1-2: Comparative Examples 1 and 2 each provide a whitening and anti-aging cosmetic product, specifically a whitening and anti-aging cream. Comparative Examples 1 and 2 each include the whitening and anti-aging compositions described in Comparative Examples 1 and 2, and their specific formulations are shown in Table 4 (total weight parts: 100 parts). The preparation method of the whitening and anti-aging cosmetic product is the same as in Example 1.

[0052] Table 4 (Unit: parts by weight) Test Example 1: Anti-wrinkle test Test samples: Whitening and anti-wrinkle creams prepared using Examples 1-3 and Comparative Example 1.

[0053] Experimental Principle: Elastin plays a crucial role in youthful skin, and its loss is a major cause of skin aging. Inhibiting elastase activity can effectively slow down the breakdown of elastin and maintain skin elasticity. Elastase can catalyze the hydrolysis of hydroxyl groups in the polypeptide bonds of various amino acids. Therefore, when it reacts with the substrate N-succinyl-alanine-alanine-alanine-p-nitroaniline, a colored substance is produced, the absorbance of which can be measured at 410 nm using a spectrophotometer. Based on the change in absorbance, the inhibitory effect of the test substance on elastase activity can be calculated, and the firming and anti-wrinkle effects of the test substance can be evaluated.

[0054] Instruments: 0.01% precision balance, microplate reader.

[0055] Reagents and materials: elastase, AAAPAN: N-succinyl-alanine-alanine-alanine-p-nitroaniline, positive control: EGCG, buffer: Tris-HCl pH=8.0.

[0056] Experimental Procedure: Elastase and the chromogenic substrate N-succinyl-alanine-alanine-p-nitroaniline were prepared as working solutions using buffer and should be prepared immediately before use. The positive control EGCG was prepared using buffer and diluted 1 / 2 times to a series of concentrations to validate the experimental system. Test Substance Treatment: The test substance was diluted with buffer to the required test concentration; otherwise, no dilution was required. Refer to the sample addition grouping list (Table 5) and conduct the experiment using 96-well microplates. The experiment included a sample group (T), a sample background group (To), an enzyme reaction group (C), and a solvent background group (C). The positive control group was treated the same as the sample group. Each group was run in triplicate.

[0057] Table 5: Sample Addition Grouping List The assay was performed using a microplate reader with the following parameters set: slow oscillation for 10 seconds, OD... 410nm Measure the absorbance. Calculate the elastase activity inhibition rate using the following formula. All experimental data are expressed as mean ± SD. Statistical methods such as the t-test can be used to analyze the data. The results of the sample's elastase activity inhibition rate are as follows: Figure 1-3 As shown in Table 6.

[0058] In the above formula: T-sample group absorbance, which is the absorbance of the solution after the sample affects the reaction of elastase and substrate AAAPAN; T0 - Absorbance of the sample background group; The absorbance of the C-enzyme reaction group, that is, the absorbance of the reaction between elastase and substrate AAAPAN without the addition of sample; C0 - absorbance of the solvent background group.

[0059] Table 6 Compared with the blank group, This indicates that P < 0.05. This indicates that P < 0.01. This indicates that P < 0.001. This indicates that P < 0.05.

[0060] The whitening and anti-wrinkle cream of Example 1 showed a 16.76±0.31% inhibition rate on elastase activity, which was statistically significant (p=0.0005).

[0061] The whitening and anti-wrinkle cream of Example 2 showed an inhibition rate of 11.24±3.24% on elastase activity, which was statistically significant (p=0.0242).

[0062] The whitening and anti-wrinkle cream of Example 3 showed an inhibition rate of 24.36±1.38% on elastase activity, which was statistically significant (p=0.0010). This indicates that the whitening and anti-wrinkle cream of the present invention has an inhibitory effect on the elastase-catalyzed decomposition of AAAPAN, and has significant firming and anti-wrinkle effects.

[0063] The whitening and anti-wrinkle cream used in Comparative Example 1 showed an inhibition rate of 5.35±0.24% on elastase activity, with no significant anti-wrinkle effect.

[0064] Test Example 2: Whitening Test Test samples: Whitening and anti-wrinkle creams prepared using Examples 1 and 3, and Comparative Example 2.

[0065] Experimental Principle: In the biosynthesis of melanin in the skin, tyrosinase is a key enzyme that acts on dopa to form dopaquinone, which then spontaneously undergoes a series of reactions to ultimately form melanin. Under suitable pH and temperature conditions, tyrosinase can catalyze the conversion of dopa to dopaquinone, and the absorbance can be measured at 475 nm using a spectrophotometer. Raw materials that inhibit tyrosinase activity can reduce the conversion of dopa to dopaquinone, thereby lowering the absorbance. The inhibitory effect of the raw materials on tyrosinase activity is evaluated based on the change in absorbance.

[0066] Instruments and equipment: microbalance, water bath, microplate reader.

[0067] Reagents and materials: disodium hydrogen phosphate-citrate buffer, pH=7.0, tyrosinase, levodopa, positive control: kojic acid.

[0068] Methods: Tyrosinase and levodopa were prepared into working solutions using buffer and stored in the dark. Positive controls were prepared into a 5 mg / mL stock solution using buffer and then diluted 1 / 2 times to create a series of concentration gradients to validate the experimental system.

[0069] Test substance preparation: The test substance is diluted with buffer solution to prepare multi-concentration samples.

[0070] Referring to the sample addition grouping list (Table 7), the experiment was conducted using 96-well microplates, establishing a sample group (T), a sample background group (To), an enzyme reaction group (C), and a solvent background group (C). The positive control group was treated the same as the sample group. Each group was run in triplicate.

[0071] Table 7 OD was detected using an enzyme-linked immunosorbent assay (ELISA) reader. 475nm Measure the absorbance. Calculate the tyrosinase activity inhibition rate using the following formula. The results of the sample's tyrosinase activity inhibition rate are as follows: Figure 4-5 As shown in Table 8.

[0072] In the above formula: T-sample group absorbance, which is the absorbance of the solution after the sample reacts with tyrosinase; T0 - Absorbance of the sample background group; The absorbance of the C-enzyme reaction group is the absorbance of the tyrosinase and dopa reaction when no sample is added. C0 - absorbance of the solvent background group.

[0073] Table 8 Tests showed that the positive control substance kojic acid exhibited a dose-response relationship in the tyrosinase-catalyzed conversion of L-DOPA to dopaquinone within the test concentration range of 0-200 μg / mL. As the concentration of the test substance increased, tyrosinase activity decreased, meaning the inhibition rate of tyrosinase activity increased, with an IC50 of 64.31 μg / mL.

[0074] The whitening and anti-wrinkle cream of Application Example 1 showed an inhibition rate of 89.68 ± 4.70% on tyrosinase activity, which was statistically significant (p = 0.0016). The whitening and anti-wrinkle cream of Application Example 3 showed an inhibition rate of 80.15 ± 4.07% on tyrosinase activity, which was statistically significant (p = 0.0008). This indicates that the whitening and anti-wrinkle cream of the present invention has an inhibitory effect on the tyrosinase-catalyzed conversion of L-DOPA to dopaquinone, and has a significant whitening effect.

[0075] The whitening and anti-wrinkle cream used in Comparative Example 2 showed an inhibition rate of 25.93 ± 6.92% on tyrosinase activity, indicating no significant inhibitory effect.

[0076] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A whitening and anti-aging composition, characterized in that, It includes the following components by weight: 0.5-15 parts Asian birch sap, 1-4 parts kava pepper root extract, and 1-4 parts mung bean seed extract.

2. The whitening and anti-aging composition according to claim 1, characterized in that, It includes the following components by weight: 8-15 parts Asian birch sap, 1-2 parts kava pepper root extract, and 1-2 parts mung bean seed extract.

3. The whitening and anti-aging composition according to claim 2, characterized in that, It includes the following components by weight: 8 parts Asian birch sap, 1 part kava pepper root extract, and 1 part mung bean seed extract.

4. The use of the whitening and anti-aging composition according to any one of claims 1-3 in the preparation of whitening and anti-aging cosmetics.

5. A whitening and anti-aging cosmetic, characterized in that, Includes the whitening and anti-aging composition as described in any one of claims 1-3.

6. The whitening and anti-aging cosmetic as described in claim 5, characterized in that, It also includes the auxiliary materials needed for cosmetics.

7. The whitening and anti-aging cosmetic as described in claim 5, characterized in that, The whitening and anti-aging composition is added in an amount of 8.5%-19% of the total mass of the whitening and anti-aging cosmetic.

8. The whitening and anti-aging cosmetic as described in claim 6, characterized in that, The excipients include at least one of the following: skin conditioning agents, emollients, moisturizers, preservatives, fragrances, emulsifiers, emulsification aids, whitening agents, and water.

9. The whitening and anti-aging cosmetic as described in claim 8, characterized in that, The skin conditioning agent includes at least one of hydroxyproline, conchiolin powder, and apple (Pyrus Malus) fiber; and / or, the emollient includes at least one of isododecane, glyceryl tri(ethylhexanoate), and isononyl isononanoate; and / or, the moisturizer includes at least one of glycerin, butylene glycol, and 1,2-hexanediol; and / or, the preservative includes phenoxyethanol; and / or, the fragrance includes a perfume; and / or, the emulsifier includes at least one of glyceryl stearate and PEG-100 stearate; and / or, the emulsifying aid includes cetearyl alcohol; the whitening agent includes 3-o-ethyl ascorbic acid.

10. The whitening and anti-aging cosmetic as described in claim 5, characterized in that, The dosage forms of the cosmetics include water, lotion, and cream.

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