Sulfur-oxidizing bacteria-mediated carbon-free autotrophic denitrification water treatment method

By acclimating sulfur-oxidizing bacteria in a gradient and optimizing reaction conditions in a partitioned fluidized bed reactor, the problem of insufficient polysulfide generation in sulfur autotrophic denitrification technology was solved, achieving efficient autotrophic denitrification of wastewater with low carbon-to-nitrogen ratio, and reducing operating costs and complexity.

CN122079354BActive Publication Date: 2026-06-26ENG CONSTR MANAGEMENT BRANCH OF NINGBO WATER ENVIRONMENT GRP CO LTD
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Patent Information

Application Number
CN202610535048.5
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2026-04-22
Publication Date
2026-06-26
Estimated Expiration
2046-04-22

AI Technical Summary

Technical Problem

Existing sulfur autotrophic denitrification technology suffers from long cultivation and acclimatization cycles for sulfur dismutating bacteria, poor environmental adaptability, and insufficient polysulfide generation capacity, resulting in low nitrogen removal efficiency and high operating costs, making it difficult to meet the needs of engineering applications.

Method used

Using the proportion of elemental sulfur and the pH of the system as dual stress factors, sulfur-oxidizing bacteria were domesticated through a gradient enhancement strategy to improve the yield of polysulfides. The reaction conditions were optimized in a partitioned fluidized bed reactor, and autotrophic denitrification was carried out using a composite sulfur source and HCO3- as electron donors.

Benefits of technology

It significantly improved the polysulfide yield and denitrification metabolic activity of sulfur-oxidizing bacteria, achieved efficient nitrogen removal from wastewater with low carbon-to-nitrogen ratio, reduced operating costs and complexity, and ensured the stability and adaptability of the system.

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Abstract

The application provides a sulfur-oxidizing bacteria-mediated carbon-free autotrophic denitrification water treatment method, and the high-performance sulfur-oxidizing bacteria with a polysulfide yield of 35%-42% is prepared through the dual-stress gradient domestication of the mass proportion of elemental sulfur in the sulfur source and the pH of the system; the high-activity bacterial suspension is prepared through centrifugal washing and buffer resuspension; the partitioned fluidized bed reactor is used, the compound sulfur source of elemental sulfur and sodium sulfide with nine waters is proportionally controlled in stages, and under the condition of no organic carbon source, the efficient autotrophic denitrification of the nitrogen-containing wastewater is realized. The denitrification efficiency is high, the operation stability is strong, no organic carbon source needs to be added, the simultaneous removal of multiple pollutants such as total phosphorus and suspended solids can be realized, the effluent quality can stably meet the high-standard discharge requirements, and the application is suitable for the advanced denitrification treatment of tail water of urban sewage treatment plants and industrial low-carbon-nitrogen-ratio wastewater.
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Citation Information

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