An exosome-stabilized cosmetic composition comprising a vitamin complex
By adding a vitamin complex to the cosmetic composition, the oxidation of exosome lipid membranes is inhibited, thus solving the problem of structural instability of exosomes in cosmetics and improving long-term storage stability and skin improvement effects.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- PLAZI CO LTD
- Filing Date
- 2026-02-26
- Publication Date
- 2026-05-29
AI Technical Summary
Exosomes in cosmetic compositions are susceptible to changes in oxygen, light, temperature, and reactive oxygen species, leading to lipid membrane oxidation, particle size changes, and leakage of contents, which affect their physiological activity and storage stability, especially when combined with other ingredients.
By incorporating vitamin complexes, particularly antioxidants such as vitamin C, vitamin E, niacinamide, and panthenol, the oxidation of exosomal lipid membranes is inhibited, and a stable cosmetic composition for skin improvement is formed by combining bone spicules, lactic acid bacteria exosomes, and ceramides.
It maintains the structural stability of exosomes, reduces particle size changes, preserves cell signal transduction function for a long time, enhances the skin barrier protection effect, and improves the storage stability and skin improvement effect of cosmetic compositions.
Smart Images
Figure CN122097230A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to an exosome-stabilized cosmetic composition comprising a vitamin complex. Background Technology
[0002] Recently, exosomes have garnered significant attention in the field of skin improvement and regeneration as physiologically active vesicles mediating intercellular signaling. Exosomes are known to contain various proteins, lipids, and nucleic acids, which can contribute to skin cell activation, promote regeneration, and alleviate inflammation. Therefore, cosmetic compositions containing exosomes have been developed and used for skin improvement purposes. However, exosomes are nanoscale vesicles with a lipid bilayer structure, and their structural stability is easily affected by external environmental conditions. In particular, exposure to oxygen, light, temperature changes, and reactive oxygen species (ROS) can oxidize the lipid membrane of exosomes, potentially leading to changes in particle size, membrane damage, or leakage of contents. Such structural changes may reduce the physiological activity of exosomes and contribute to functional decline during long-term storage. While previous cosmetic compositions containing antioxidants have been proposed, these primarily aim to alleviate oxidative stress on the skin surface, with relatively insufficient design of compositions aimed at maintaining the structural stability of exosomes themselves. Furthermore, for composite compositions containing both exosomes and other active ingredients, there is a possibility that the physical and chemical stability of exosomes may decrease during storage due to interactions between the components. Therefore, there is a need for a technology that can improve structural stability, thereby maintaining the physiological activity of exosomes over a long period and ensuring stable function when applied to the skin. In particular, there is a need for a composition design that can inhibit exosome lipid membrane oxidation and minimize particle size variation. In view of this, the present invention aims to provide a cosmetic composition for improving skin stabilization by incorporating a vitamin complex, which reduces oxidative damage to exosomes and improves structural stability. Summary of the Invention
[0003] This invention aims to address the structural instability issues that may occur in cosmetic compositions containing exosomes. Specifically, exosomes are nanovesicles with a lipid bilayer structure, which may undergo lipid membrane oxidation, particle size changes, and content leakage when exposed to oxygen, light, temperature variations, and reactive oxygen species (ROS). These changes can reduce the cell signaling function of exosomes, leading to a decrease in skin-improving effects. Particularly for complex compositions containing other active ingredients such as bone spurs and ceramides, the stability of exosomes may further decrease during storage due to physical and chemical interactions. Therefore, the objective of this invention is to inhibit lipid membrane oxidation of exosomes and improve structural stability by incorporating a vitamin complex, thereby providing an exosome-stabilized cosmetic composition for skin improvement that maintains physiological activity even during long-term storage.
[0004] According to an embodiment of the present invention, a cosmetic composition for improving skin by stabilizing exosomes is a cosmetic composition for improving skin by stabilizing exosomes containing a vitamin complex. It comprises spicules, lactic acid bacteria exosomes, and ceramides, wherein the spicules, lactic acid bacteria exosomes, and ceramides are contained in a weight ratio of 0.6:3.0:0.01. The lactic acid bacteria exosomes comprise a substance obtained by concentrating Lactobacillus curvatus GFC-AM5 strain (gateway number KCCM12245P) at 37°C for 20 hours at a concentration of 40 times or more. Based on the total weight of the composition, it contains 0.001 to 5% by weight of the vitamin complex. The vitamin complex comprises two or more selected from the group consisting of vitamin C or its derivatives, vitamin E or its derivatives, niacinamide, and panthenol. The vitamin complex is characterized by comprising the following proportions: 0.001 to 3 wt% vitamin C derivative, 0.001 to 2 wt% vitamin E or tocopherol, 0.01 to 3 wt% niacinamide, and 0.01 to 3 wt% panthenol. The vitamin complex improves long-term storage stability by inhibiting particle size changes in lactic acid bacteria exosomes or reducing oxidative damage. Furthermore, cosmetics according to the invention comprise creams, lotions, serums, ampoules, or mask formulations containing exosome-stabilized skin-improving cosmetic compositions.
[0005] The exosome-stabilized skin-improving cosmetic composition according to the present invention has the following effects. First, the antioxidant components contained in the vitamin complex inhibit the oxidation of the lipid bilayer of exosomes, thereby reducing particle size changes and structural damage during storage. Second, as the structural stability of exosomes is maintained, cell signaling function is preserved, thus maintaining the skin cell activation effect for a long time. Third, the stabilized exosomes are delivered into the skin through microchannels formed by bone needles, thereby effectively manifesting skin-improving effects. Fourth, the interaction between ceramides and the vitamin complex stabilizes the skin barrier, enhancing the protective effect against external oxidative stress. Therefore, the present invention is not merely a simple antioxidant composition, but a composition designed to maintain the structural stability and functional preservation of exosomes, providing improved storage stability and skin-improving effects compared to existing exosome-containing cosmetic compositions. Attached Figure Description
[0006] Figure 1 is a schematic diagram illustrating the mechanism of action of a cosmetic composition for improving skin by stabilizing exosomes containing a vitamin complex, according to an embodiment of the present invention.
[0007] Figure 2 is a graph showing the results of NTA (nanoparticle tracking analysis) analysis of the change in average exosome size after storing a cosmetic composition for skin improvement containing an exosome stabilization complex and a comparative composition without an exosome stabilization complex for a certain period of time, according to an embodiment of the present invention.
[0008] Figure 3 is a graph showing the comparison of the maintenance rate of exosome particle structure under oxidative stress conditions based on whether or not it contains a vitamin complex. Detailed Implementation
[0009] Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings, so that those skilled in the art can readily implement the invention. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. For the purpose of clearly illustrating the invention in the drawings, parts unrelated to the description have been omitted, and the same reference numerals are used throughout the specification for the same or similar constituent elements. In this specification, terms such as "comprising" or "having" are intended to specify the presence of features, numbers, steps, operations, constituent elements, components, or combinations thereof recorded in the specification, and should be understood as not precluding the possibility of the presence or addition of one or more other features, numbers, steps, operations, constituent elements, components, or combinations thereof. Furthermore, when referring to a layer, film, region, plate, etc., being "above" another part, this includes not only the case where it is "directly above" the other part, but also the case where the other part exists in between. Conversely, when referring to a layer, film, region, plate, etc., being "below" another part, this includes not only the case where it is "directly below" the other part, but also the case where the other part exists in between.
[0010] Figure 1 is a schematic diagram illustrating the mechanism of action of a cosmetic composition for skin improvement containing an exosome-stabilized vitamin complex according to an embodiment of the present invention. Referring to Figure 1, exosomes are vesicles with a lipid bilayer structure. When exposed to reactive oxygen species (ROS), they can induce membrane oxidation, which may lead to changes in particle size or decreased function. In contrast, in the composition of the present invention, the vitamin complex is present around the exosomes, which can maintain the structural stability of the exosomes by alleviating oxidative stress and inhibiting lipid membrane oxidation. As a result, changes in the particle size of the exosomes are suppressed, and cell signaling function can be maintained for a long time. The cosmetic composition for skin improvement containing an exosome according to the present invention comprises spicules, lactic acid bacteria exosomes, ceramides, and a vitamin complex. The spicules, lactic acid bacteria exosomes, and ceramides are preferably contained in a weight ratio of 0.6:3.0:0.01. The weight ratio is determined based on a composition ratio that has been proven to have excellent skin delivery efficiency and cell activity effects. As the lactic acid bacteria exosomes, a substance obtained by culturing Lactobacillus curvatus GFC-AM5 strain (host number KCCM12245P) at 37°C for 20 hours and then concentrating the culture medium more than 40 times can be used. The spicules can be components derived from hydrolyzed sponges, which can promote exosome delivery by forming microchannels on the skin surface. The ceramide can be yeast-derived ceramide NP, which provides functions such as strengthening the skin barrier and maintaining moisture. In this invention, the vitamin complex is an antioxidant component for inhibiting the oxidation of the exosome lipid bilayer, and may contain two or more components selected from the group consisting of vitamin C or its derivatives, vitamin E or its derivatives, nicotinamide, and panthenol. The vitamin complex can be composed of a combination of two or more components selected from the group, and may also contain three or four components simultaneously as needed. By using different water-soluble and fat-soluble vitamins in combination, the oxidative inhibitory effect on the exosome lipid bilayer can be mutually enhanced. Based on the total weight of the composition, the vitamin complex preferably comprises 0.001 to 5% by weight, more preferably in the range of 0.01 to 3% by weight. Below the lower limit, the inhibitory effect on exosome membrane oxidation may not be fully realized; above the upper limit, further improvement in stability may not be significantly increased. Specifically, the content of vitamin C or its derivatives may range from 0.001 to 3% by weight, and the content of vitamin E or tocopherol may range from 0.001 to 2% by weight. Furthermore, the content of nicotinamide may range from 0.01 to 5% by weight, and the content of panthenol may range from 0.01 to 5% by weight. These ranges are designed to maintain dosage form stability while inhibiting exosome lipid membrane oxidation.Referring to Figure 1, exosomes are vesicles with a lipid bilayer structure. Exposure to reactive oxygen species (ROS) induces lipid membrane oxidation, potentially leading to changes in particle size and functional decline. The vitamin complex according to the invention inhibits the oxidation of the exosome lipid membrane through antioxidant action and reduces particle size changes during storage by maintaining the integrity of the membrane structure. Thus, the cell signaling function of exosomes can be maintained long-term. Therefore, the composition according to the invention can inhibit the rate of increase in the average particle size of exosomes and reduce the expansion of the particle size distribution width under long-term storage conditions. Furthermore, even under oxidative stress conditions, the structural integrity of exosomes can be maintained, thereby minimizing the decline in cell signaling function. A basic composition is prepared by uniformly mixing the bone spicules, lactic acid bacteria exosomes, and ceramides in the stated weight ratio. Subsequently, the vitamin complex is added within a set content range and uniformly mixed under low-speed stirring. Considering skin compatibility, the pH of the composition can be adjusted to a weakly acidic range, and excessively high temperatures or strong oxidizing conditions are preferably avoided during preparation.
[0011] Example 1: Preparation of a composition containing a vitamin complex. A basic composition was prepared by mixing bone spicules, lactic acid bacteria exosomes, and ceramides in a weight ratio of 0.6:3.0:0.01. Subsequently, 0.1% by weight of a vitamin C derivative, 0.05% by weight of tocopherol, 1.0% by weight of nicotinamide, and 0.5% by weight of panthenol were added, and the mixture was stirred uniformly to prepare the composition of the present invention.
[0012] Comparative Example 1: A composition without vitamin complex was prepared as a base composition in the same manner as in Example 1 above, but without the addition of vitamin complex.
[0013] Experimental Example 1: Analysis of Exosome Particle Size Changes During Storage The compositions of Example 1 and Comparative Example 1 were stored at 25°C for 4 weeks, and the average particle size of the exosomes was analyzed using NTA (nanoparticle tracking analysis). The results showed that in Comparative Example 1, an increase in average particle size and an expansion of the distribution width were observed during storage, but in Example 1, the particle size change was relatively small. Figure 2 is a graph showing the results of NTA (nanoparticle tracking analysis) analysis of the changes in the average particle size of exosomes after a certain period of storage of a cosmetic composition for skin improvement containing an exosome containing a vitamin complex and a comparative composition without a vitamin complex, according to an embodiment of the present invention. Referring to Figure 2, in the case of the comparative example without the vitamin complex, the average particle size showed a gradual increasing trend with the passage of storage time. This indicates that the lipid bilayer of the exosomes may undergo oxidation or partial aggregation, resulting in structural deformation. In particular, the increase in average particle size was significant after 4 weeks of storage, confirming that structural stability may decrease during long-term storage. Conversely, in the embodiments containing the vitamin complex, the variation in average particle size was relatively small even under the same storage conditions. This means that the vitamin complex effectively suppressed the particle size increase rate by inhibiting the oxidation of the lipid bilayer of exosomes and maintaining the integrity of the membrane structure. Therefore, Figure 2 supports the technical effect of the vitamin complex according to the invention in improving the long-term storage stability of exosomes.
[0014] Example 2: Stability Evaluation under Oxidative Stress Conditions. The changes in exosome particle structure under oxidative conditions (hydrogen peroxide treatment) were analyzed. Results showed that, compared to Comparative Example 1, Example 1 containing the vitamin complex exhibited reduced lipid membrane damage and higher particle size maintenance. Figure 3 is a graph showing the comparison of exosome particle structure maintenance under oxidative stress conditions based on whether or not the vitamin complex was included. Referring to Figure 3, when exposed to oxidative conditions (e.g., hydrogen peroxide treatment), the average particle size maintenance of exosomes decreased in the comparative example without the vitamin complex. This suggests that reactive oxygen species may damage the lipid membrane of exosomes, leading to particle structure deformation or partial aggregation. In contrast, in the example containing the vitamin complex, the particle size maintenance remained at a high level even under oxidative conditions. This indicates that antioxidants such as vitamin C or its derivatives, vitamin E or its derivatives, nicotinamide, and panthenol protected the lipid bilayer of exosomes, thereby reducing oxidative damage. In other words, Figure 3 shows that the composition according to the present invention is not limited to simply relieving oxidative stress on the skin surface, but can stably preserve cell signal transduction function by maintaining the structural integrity of the exosomes themselves.
[0015] The compositions according to the present invention can be prepared into various cosmetic formulations such as creams, lotions, serums, ampoules, masks, or gels. The vitamin complex contained in the composition can improve storage stability by maintaining the structural stability of exosomes, and the stabilized exosomes are delivered into the skin through microchannels formed by bone needles, effectively demonstrating skin improvement effects.
[0016] The embodiments of the present invention have been described above. However, the concept of the present invention is not limited to the embodiments presented in this specification. Those skilled in the art who understand the concept of the present invention can easily propose other embodiments by adding, changing, deleting, or adding constituent elements within the same scope of the concept. These also fall within the scope of the present invention.
Claims
1. A cosmetic composition for improving skin by stabilizing exosomes containing a vitamin complex, characterized in that, The composition comprises bone spicules, lactic acid bacteria exosomes, and ceramides, wherein the bone spicules, lactic acid bacteria exosomes, and ceramides are contained in a weight ratio of 0.6:3.0:0.
01. The lactic acid bacteria exosomes comprise a substance obtained by concentrating Lactobacillus curvatus GFC-AM5 strain (hostage number KCCM12245P) at 37°C for 20 hours at a concentration of more than 40 times. The composition contains 0.001 to 5% by weight of the vitamin complex based on the total weight of the composition.
2. The cosmetic composition for improving skin by stabilizing exosomes containing a vitamin complex according to claim 1, characterized in that, The vitamin complex comprises two or more selected from the group consisting of vitamin C or its derivatives, vitamin E or its derivatives, nicotinamide and panthenol.
3. The cosmetic composition for improving skin by stabilizing exosomes containing a vitamin complex according to claim 1 or 2, characterized in that, The vitamin complex comprises the following components: 0.001 to 3% by weight of vitamin C derivative, 0.001 to 2% by weight of vitamin E or tocopherol, 0.01 to 3% by weight of nicotinamide, and 0.01 to 3% by weight of panthenol.