A preparation for improving the germination rate of radix paeoniae rubra seeds, a preparation method thereof and application thereof
By preparing a formulation containing specific ingredients, the problems of low germination rate of red peony seeds and inconsistent seedling quality were solved, thus realizing efficient propagation and large-scale production of red peony.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- JILIN ACAD OF AGRI SCI
- Filing Date
- 2025-06-27
- Publication Date
- 2026-06-26
AI Technical Summary
The germination rate, seedling emergence rate, and survival rate of red peony seeds are low, and the quality of seedlings is inconsistent. Existing propagation methods have long production cycles and low propagation coefficients, making them unsuitable for large-scale promotion.
A formulation containing potassium dihydrogen phosphate, urea, vitamin C, amino acid complex, trace elements, plant extracts, film-forming substances, dispersants, and thickeners is prepared by uniform mixing. This formulation provides the nutrients and antioxidant and antibacterial protection required for the germination of Paeonia lactiflora seeds, promoting seed germination and root development.
It significantly improves the germination and emergence rate of red peony, enhances the growth and quality of seedlings, solves the problems of low seed germination rate and inconsistent seedling quality, and promotes the large-scale planting of red peony.
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Figure BDA0005471655490000091 
Figure BDA0005471655490000101
Abstract
Description
Technical Field
[0001] This invention relates to the field of traditional Chinese medicine cultivation technology, and in particular to a preparation for improving the germination rate of Paeonia lactiflora seeds, its preparation method, and its application. Background Technology
[0002] Red peony (Paeonia lactiflora Pall.) is a wild Chinese medicinal herb with a long history of efficacy. It enjoys high and stable demand, multiple uses, and substantial export volume. Also known as Jiangli, Lishi, or Licao, red peony belongs to the dicotyledonous class, Ranunculales order, Ranunculaceae family, and Paeonia genus. Its dried roots are widely used as a medicinal material.
[0003] Red peony root contains abundant physiologically active components such as glycosides, phenolic acids, monoterpenes, and peptides. It also contains certain amounts of erucic acid, palmitic acid, and tannins. With ongoing research, it has been discovered that red peony root can promote the immune system, fight inflammation, relieve spasms, and prevent convulsions. Furthermore, it has a significant effect on inhibiting platelet and erythrocyte aggregation, contributing to anticoagulation, antithrombosis, and anti-atherosclerosis efforts. It is also beneficial to the heart and can promote healthy cardiopulmonary function. Therefore, there is a huge market demand for it.
[0004] Scholars both domestically and internationally have conducted extensive research on peony propagation techniques, but most studies focus on asexual reproduction of peony tubers, such as division, along with cuttings and layering. The biggest drawbacks are the long production cycle, low propagation coefficient, and limited resources available for division, hindering large-scale implementation. Currently, sowing red peony seeds is also possible, but this method suffers from low germination rates, low seedling emergence rates, low seedling survival rates, and inconsistent quality of the final product. Summary of the Invention
[0005] The purpose of this invention is to provide a preparation for improving the germination rate of Paeonia lactiflora seeds, its preparation method, and its application, so as to solve the problems existing in the prior art.
[0006] To achieve the above objectives, the present invention provides the following solution:
[0007] One of the technical solutions of the present invention is a preparation for improving the germination rate of Paeonia lactiflora seeds, comprising the following raw materials in parts by weight: 5-6 parts potassium dihydrogen phosphate, 6-7 parts urea, 1-1.5 parts vitamin C, 1-1.5 parts amino acid complex, 3-4 parts trace elements, 8-10 parts plant extract, 3-4 parts film-forming substance, 1-2 parts dispersant and 0.01-0.02 parts thickener.
[0008] Further, the amino acid complex comprises the following components in parts by weight: 4-6 parts aspartic acid, 2-3 parts arginine, 5-8 parts glutamic acid, 2-3 parts alanine, and 4-6 parts γ-aminobutyric acid.
[0009] Furthermore, the trace elements include the following components in parts by mass: 4-6 parts zinc sulfate, 2-3 parts manganese sulfate, 2-3 parts copper sulfate, 1-2 parts sodium selenite, and 3-4 parts potassium borate;
[0010] The film-forming substance includes sodium polyacrylate;
[0011] The dispersant includes sodium dodecylbenzenesulfonate;
[0012] The thickener includes xanthan gum.
[0013] Furthermore, the plant extract comprises the following components in parts by weight: 5-8 parts grape skin extract, 10-12 parts honeysuckle and chia seed mixed extract, 13-15 parts dandelion and garlic mixed extract, and 2-3 parts holly branch and leaf extract.
[0014] Furthermore, the method for preparing the grape skin extract includes the following steps:
[0015] The grape skins and ethanol solution were mixed evenly, heated and refluxed for extraction, and then concentrated and dried to obtain the grape skin extract.
[0016] Furthermore, the preparation method of the honeysuckle and chia seed mixed extract includes the following steps:
[0017] Honeysuckle was soaked in an ethanol solution, then sonicated, filtered, concentrated, and dried to obtain honeysuckle extract.
[0018] Chia seeds and an ethanol solution were mixed and heated to extract the extract. The ethanol was removed from the extract, a carbon source was added, and fermentation bacteria were inoculated. After fermentation, the solid and liquid were separated, and the liquid was dried to obtain the chia seed extract.
[0019] The honeysuckle extract and chia seed extract were mixed evenly to obtain the honeysuckle and chia seed mixed extract.
[0020] Furthermore, the preparation method of the dandelion and garlic mixed extract includes the following steps:
[0021] Dandelion, garlic and water were mixed, heated and refluxed for extraction, and then the solid and liquid were separated. The extract was concentrated and dried to obtain the dandelion and garlic mixed extract.
[0022] Furthermore, the preparation method of the holly branch and leaf extract includes the following steps:
[0023] Holly branches and leaves were crushed and added to water. After soaking, solid-liquid separation was performed, and the extract was concentrated and dried to obtain the holly branch and leaf extract.
[0024] The nutrient solution of this invention contains potassium dihydrogen phosphate, urea, vitamin C, amino acid complex, trace elements, and plant extracts, which can provide nutrients for the germination of Paeonia lactiflora seeds. In addition, the grape skin extract in this invention is rich in resveratrol, and the mixed extract of honeysuckle and chia seeds is rich in chlorogenic acid and caffeic acid, which have excellent antioxidant effects. These can reduce the sensitivity of Paeonia lactiflora seeds to the environment, significantly promote the recovery of the vitality of Paeonia lactiflora seeds, and thus greatly improve the germination rate and germination quality of Paeonia lactiflora seeds.
[0025] The mixed extract of dandelion and garlic has excellent antibacterial properties and can effectively prevent pathogens carried by peony seeds. It can not only prevent peony seeds from being damaged by peony pathogens, but also prevent the peony seedlings prepared from being diseased due to the pathogens carried by the peony seeds, thus avoiding the phenomenon that the peony seedlings are diseased and thus affect the later growth and development of peony.
[0026] Extracts from holly branches and leaves can promote the rooting and germination of red peony seeds, thereby improving the germination rate of red peony seeds and the quality of seedlings.
[0027] The second technical solution of the present invention: a method for preparing the above-mentioned preparation for improving the germination rate of Paeonia lactiflora seeds, comprising the following steps:
[0028] Potassium dihydrogen phosphate, urea, vitamin C, amino acid complex, trace elements, plant extracts, film-forming substances, dispersants, and thickeners are mixed evenly to obtain the preparation for improving the germination rate of Paeonia lactiflora seeds.
[0029] The third technical solution of the present invention: the application of the above-mentioned preparation for improving the germination rate of red peony seeds in the cultivation of red peony.
[0030] The present invention discloses the following technical effects:
[0031] The formulation of this invention can significantly improve the germination rate and emergence rate of Paeonia lactiflora, enhance the growth of Paeonia lactiflora seedlings, and effectively solve the problems of low seed germination rate and inconsistent seedling quality of Paeonia lactiflora. Detailed Implementation
[0032] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.
[0033] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.
[0034] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.
[0035] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be obvious to those skilled in the art. This specification and embodiments are merely exemplary.
[0036] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.
[0037] It should be noted that any aspects not described in detail in this invention are conventional practices in the field and are not the focus of this invention.
[0038] In the following examples, "parts" refers to "parts by weight".
[0039] In the specific embodiments of the present invention, the brewing yeast used was purchased from the China Industrial Microbial Culture Collection Center, product number CICC 1309.
[0040] The specific embodiment of the present invention uses the following method for preparing brewing yeast:
[0041] Saccharomyces cerevisiae was inoculated into MEA liquid medium for activation culture, followed by fermentation culture, yielding a bacterial count of 1.0 × 10⁻⁶. 9 Saccharomyces cerevisiae culture at cfu / mL.
[0042] Example 1
[0043] A method for preparing a formulation to improve the germination rate of Paeonia lactiflora seeds:
[0044] (1) The preparation for improving the germination rate of Paeonia lactiflora seeds is composed of the following raw materials in parts by weight: 6 parts potassium dihydrogen phosphate, 7 parts urea, 1.2 parts vitamin C, 1.5 parts amino acid complex, 3.5 parts trace elements, 10 parts plant extract, 4 parts film-forming substance, 1.5 parts dispersant and 0.01 parts thickener.
[0045] The amino acid complex is composed of the following components in parts by mass: 5 parts aspartic acid, 3 parts arginine, 8 parts glutamic acid, 2 parts alanine, and 4 parts γ-aminobutyric acid.
[0046] The trace elements are composed of the following components in parts by mass: 6 parts zinc sulfate, 2 parts manganese sulfate, 3 parts copper sulfate, 1 part sodium selenite, and 4 parts potassium borate.
[0047] The film-forming substance is sodium polyacrylate, with a weight-average molecular weight of approximately 20,000.
[0048] The dispersant is sodium dodecylbenzenesulfonate;
[0049] The thickener is xanthan gum;
[0050] The plant extract consists of the following components in parts by weight: 6 parts grape skin extract, 12 parts honeysuckle and chia seed mixed extract, 15 parts dandelion and garlic mixed extract, and 3 parts holly branch and leaf extract.
[0051] (2) Preparation method of grape skin extract
[0052] The grape skins were dried and then pulverized to obtain grape skin powder (particle size 100 mesh). The grape skin powder was mixed with a 70 vol.% ethanol solution (the ratio of grape skin powder to ethanol solution was 1 g: 15 mL), and the mixture was refluxed at 80 °C for 2 h. The extract was then concentrated and dried to obtain the grape skin extract.
[0053] (3) Preparation method of honeysuckle and chia seed mixed extract
[0054] Honeysuckle was dried and pulverized to obtain honeysuckle powder (particle size 100 mesh). The honeysuckle powder was soaked in a 70 vol.% ethanol solution (the ratio of honeysuckle powder to ethanol solution was 1 g: 15 mL) at room temperature (25℃) for 48 h, and then subjected to ultrasonic treatment for 60 min (power 500 W, temperature 60℃). After filtration, the extract was concentrated and dried to obtain honeysuckle extract.
[0055] Chia seeds were dried and pulverized to obtain chia seed powder (particle size 100 mesh). The chia seed powder was mixed with a 60 vol.% ethanol solution (the ratio of chia seed powder to ethanol solution was 1 g: 20 mL), and extracted by heating (temperature 80℃, time 2 h). After removing the ethanol, the extract was obtained. Glucose was added to the extract (the ratio of glucose to extract was 1 g: 100 mL), and then fermentation bacteria (Saccharomyces cerevisiae liquid, inoculation amount of Saccharomyces cerevisiae liquid was 0.1 vol.%) were inoculated. Fermentation was carried out (temperature 37℃, time 72 h). After solid-liquid separation, the liquid was dried to obtain chia seed extract.
[0056] Honeysuckle extract and chia seed extract were mixed evenly at a mass ratio of 1:1 to obtain a mixed extract of honeysuckle and chia seeds.
[0057] (4) Preparation method of dandelion and garlic mixed extract
[0058] Dandelion and garlic were dried and pulverized separately to obtain dandelion powder (particle size 100 mesh) and garlic powder (particle size 100 mesh). The dandelion powder and garlic powder were mixed at a mass ratio of 1:1 to obtain a mixed powder. The mixed powder and water were mixed at a volume ratio of 1g:20mL, and the mixture was heated and refluxed for extraction (temperature 100℃, time 4h). After solid-liquid separation, the extract was concentrated and dried to obtain a mixed extract of dandelion and garlic.
[0059] (5) Preparation method of holly branch and leaf extract
[0060] Holly branches and leaves were dried and pulverized to obtain holly branch and leaf powder (particle size 100 mesh); the holly branch and leaf powder was added to water (the ratio of holly branch and leaf powder to water was 1g:30mL) and soaked for 48h. After filtration, the extract was obtained. The extract was concentrated and dried to obtain holly branch and leaf extract.
[0061] (6) Mix potassium dihydrogen phosphate, urea, vitamin C, amino acid complex, trace elements, plant extracts, film-forming substances, dispersants and thickeners evenly to obtain a preparation that improves the germination rate of Paeonia lactiflora seeds.
[0062] Example 2
[0063] A method for preparing a formulation to improve the germination rate of Paeonia lactiflora seeds:
[0064] (1) The preparation for improving the germination rate of Paeonia lactiflora seeds is composed of the following raw materials in parts by weight: 5 parts potassium dihydrogen phosphate, 6 parts urea, 1 part vitamin C, 1 part amino acid complex, 3 parts trace elements, 8 parts plant extract, 3 parts film-forming substance, 2 parts dispersant and 0.02 parts thickener.
[0065] The amino acid complex is composed of the following components in parts by mass: 4 parts aspartic acid, 2 parts arginine, 6 parts glutamic acid, 3 parts alanine, and 5 parts γ-aminobutyric acid.
[0066] The trace elements are composed of the following components in parts by mass: 4 parts zinc sulfate, 3 parts manganese sulfate, 2 parts copper sulfate, 2 parts sodium selenite, and 3 parts potassium borate.
[0067] The film-forming substance is sodium polyacrylate, with a weight-average molecular weight of approximately 20,000.
[0068] The dispersant is sodium dodecylbenzenesulfonate;
[0069] The thickener is xanthan gum;
[0070] The plant extract consists of the following components in parts by weight: 8 parts grape skin extract, 10 parts honeysuckle and chia seed mixed extract, 14 parts dandelion and garlic mixed extract, and 2 parts holly branch and leaf extract.
[0071] Steps (2) to (6) are the same as in Example 1.
[0072] Comparative Example 1
[0073] Same as Example 1, except that the preparation for improving the germination rate of Paeonia lactiflora seeds does not contain film-forming substances.
[0074] Comparative Example 2
[0075] Same as Example 1, except that the preparation for improving the germination rate of Paeonia lactiflora seeds does not contain amino acid complexes.
[0076] Comparative Example 3
[0077] Same as Example 1, except that the plant extract does not contain grape skin extract and holly branch and leaf extract.
[0078] Comparative Example 4
[0079] Same as Example 1, except that the dandelion and garlic mixed extract is replaced with the mugwort and houttuynia cordata mixed extract, and the preparation method is the same as that for the dandelion and garlic mixed extract.
[0080] Comparative Example 5
[0081] Same as Example 1, except that the preparation for improving the germination rate of Paeonia lactiflora seeds does not contain plant extracts.
[0082] Example 3
[0083] A method for cultivating red peony:
[0084] (1) Select plump, glossy, disease-free, and harvested red peony (Duolun red peony) seeds from the previous year. Soak them in 50℃ warm water for 24 hours, then change the water and soak them in cool water for 48 hours (changing the water once during this period). After draining the water, soak them in the solution of the preparation prepared in the example or comparative example (the mass ratio of preparation to water is 1:50) for 30 minutes. After taking them out, let them air dry naturally to form a film, and obtain red peony seeds for sowing.
[0085] (2) In late September, the seeds of red peony treated in step (1) were sown using the row sowing method (the width of the seedbed is 0.8m, the distance between the seedbeds is 0.3cm, and the row spacing of the seeds is 20cm). 200 seeds of different treatments were sown. After sowing, the soil should be covered with 4-5cm of soil immediately, and the seedbed surface should be leveled before covering with mulch.
[0086] Seedlings will emerge in early May of the second year. The mulch should be removed in time before emergence. The emergence rate should be counted at the end of May, and the plant height and stem diameter (circumference of the main stem) should be counted in early July. The results are shown in Table 1.
[0087] Comparative Example 6
[0088] A method for cultivating red peony:
[0089] (1) Select plump, glossy, disease-free, and newly harvested red peony (Duolun red peony) seeds. Soak them in 50℃ warm water for 24 hours, then change the water and soak them in cool water for 48 hours (changing the water once during this period). After draining the water, mix the seeds with a 400-fold dilution of 25% carbendazim wettable powder and let them air dry naturally to obtain red peony seeds for sowing.
[0090] (2) In late September, use the row sowing method (seedbed width is 0.8m, the distance between seedbeds is 0.3cm, and the row spacing of seeds is 20cm) to sow the red peony seeds treated in step (1) (sow 200 seeds). After sowing, cover the seedbed with 4-5cm of soil immediately, and then cover the seedbed with mulch after leveling the seedbed surface.
[0091] Seedlings will emerge in early May of the second year. The mulch should be removed in time before emergence. The emergence rate should be counted at the end of May, and the plant height and stem diameter (circumference of the main stem) should be counted in early July. The results are shown in Table 1.
[0092] Table 1 Emergence rate, plant height, and stem diameter
[0093]
[0094]
[0095] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.
Claims
1. A preparation for improving the germination rate of Paeonia lactiflora seeds, characterized in that, The raw materials include the following parts by weight: 5-6 parts potassium dihydrogen phosphate, 6-7 parts urea, 1-1.5 parts vitamin C, 1-1.5 parts amino acid complex, 3-4 parts trace elements, 8-10 parts plant extracts, 3-4 parts film-forming substances, 1-2 parts dispersant and 0.01-0.02 parts thickener; The plant extract comprises the following components in parts by weight: 6-8 parts grape skin extract, 10-12 parts honeysuckle and chia seed mixed extract, 14-15 parts dandelion and garlic mixed extract, and 2-3 parts holly branch and leaf extract. The method for preparing the grape skin extract includes the following steps: The grape skins and ethanol solution were mixed evenly and then heated under reflux for extraction. The extract was then concentrated and dried to obtain the grape skin extract. The preparation method of the honeysuckle and chia seed mixed extract includes the following steps: Honeysuckle was soaked in an ethanol solution, then sonicated, filtered, concentrated, and dried to obtain honeysuckle extract. Chia seeds and an ethanol solution were mixed and heated to extract the extract. The ethanol was removed from the extract, a carbon source was added, and fermentation bacteria were inoculated. After fermentation, the solid and liquid were separated, and the liquid was dried to obtain the chia seed extract. The honeysuckle extract and chia seed extract were mixed evenly to obtain the honeysuckle and chia seed mixed extract. The fermenting bacteria is brewer's yeast; The preparation method of the dandelion and garlic mixed extract includes the following steps: Dandelion, garlic and water were mixed, heated under reflux for extraction, and then solid-liquid separation was performed. The extract was concentrated and dried to obtain the dandelion and garlic mixed extract. The preparation method of the holly branch and leaf extract includes the following steps: Holly branches and leaves were crushed and added to water. After soaking, solid-liquid separation was performed, and the extract was concentrated and dried to obtain the holly branch and leaf extract.
2. The formulation according to claim 1, characterized in that, The amino acid complex comprises the following components in parts by weight: 4-6 parts aspartic acid, 2-3 parts arginine, 5-8 parts glutamic acid, 2-3 parts alanine, and 4-6 parts γ-aminobutyric acid.
3. The formulation according to claim 1, characterized in that, The trace elements include the following components in parts by weight: 4-6 parts zinc sulfate, 2-3 parts manganese sulfate, 2-3 parts copper sulfate, 1-2 parts sodium selenite, and 3-4 parts potassium borate; And / or, the film-forming substance includes sodium polyacrylate; And / or, the dispersant comprises sodium dodecylbenzenesulfonate; And / or, the thickener includes xanthan gum.
4. A method for preparing the formulation for improving the germination rate of Paeonia lactiflora seeds according to any one of claims 1 to 3, characterized in that, Includes the following steps: Potassium dihydrogen phosphate, urea, vitamin C, amino acid complex, trace elements, plant extracts, film-forming substances, dispersants, and thickeners are mixed evenly to obtain the preparation for improving the germination rate of Paeonia lactiflora seeds.
5. The application of the preparation for improving the germination rate of Paeonia lactiflora seeds as described in any one of claims 1 to 3 in the cultivation of Paeonia lactiflora.