Preparation and application of ratio type sensor for synchronously detecting total hemoglobin and glycosylated hemoglobin
By modifying a glassy carbon electrode with GO-CNT/AuNPs composite material and immobilizing anthraquinone molecules, combined with antibody-specific capture, a ratiometric electrochemical sensor with "dual signal on" was constructed. This solved the accuracy problem of simultaneous detection of tHb and HbA1c in the prior art, and realized the simultaneous quantitative analysis of tHb and HbA1c on a single electrode, which is suitable for rapid determination of whole blood samples.
Patent Information
- Application Number
- CN202610362352.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-03-24
- Publication Date
- 2026-05-29
AI Technical Summary
Existing electrochemical detection methods are difficult to achieve simultaneous and accurate determination of total hemoglobin (tHb) and glycated hemoglobin (HbA1c) at a single electrode interface, and are easily affected by non-specific adsorption and matrix interference in complex biological sample systems.
A ratiometric electrochemical sensor with "dual signal on" was constructed. By modifying a glassy carbon electrode with GO-CNT/AuNPs composite material, anthraquinone (AQ) molecules were immobilized and bound to an antihemoglobin antibody. The reduction peak currents of ferrocene boric acid (FcBA) and thionine (Thi) were measured using differential pulse voltammetry (DPV), enabling simultaneous quantitative analysis of tHb and HbA1c.
It enables simultaneous and accurate quantification of tHb and HbA1c on a single electrode, reduces the influence of non-specific adsorption and background fluctuations in complex biological matrices, improves the accuracy and repeatability of detection, and is suitable for rapid determination of whole blood samples.