An oil composition, a method for preparing the same, use thereof, and a skin care product

Abstract

This invention discloses an oil composition, its preparation method, applications, and skincare products. The oil composition includes coffee oil, grape seed oil, and coconut oil. This invention uses supercritical fluid extraction to extract coffee oil, grape seed oil, and coconut oil. These natural oils have high bioavailability and can be effectively absorbed by the skin, thereby improving skincare effects. Compared to synthetic oils and waxes and naturally derived oil extracts, the bioavailability of this invention is significantly improved. Furthermore, the oil composition of this invention is safe and has anti-inflammatory, soothing, whitening, and anti-vasodilatory effects, effectively improving dark circles under the eyes.

Description

Technical Field

[0001] This invention relates to an oil composition, its preparation method, application, and skin care products. Background Technology

[0002] In the field of cosmetic technology, the research and development of skincare and beauty products has always been a hot topic. Oil-soluble ingredients are commonly used in skincare products, effectively moisturizing and caring for the skin. However, most existing oil-soluble ingredients are synthetic oils and waxes, offering limited skincare benefits and failing to meet the diverse needs of skincare products. In addition, naturally derived oil extracts are gaining popularity in the market due to their rich active ingredients and good biocompatibility. Current technology not only focuses on the extraction and application of single oils, but also increasingly emphasizes the skincare effects of blends of multiple oils.

[0003] Green coffee beans are unroasted, raw coffee beans. Their chemical composition differs from roasted coffee beans, therefore, the two types of coffee are used differently. The general health benefits of green coffee bean extract are attributed to its unique polyphenols, with chlorogenic acid being the most important (12-18% dry weight in green coffee). Green coffee beans are the richest source of chlorogenic acid. However, during the coffee bean roasting process, chlorogenic acid undergoes degradation and transformation, leading to a decrease in its content in roasted coffee. Current research focuses on the antioxidant and anti-inflammatory activities of chlorogenic acid. Common extraction and separation techniques for chlorogenic acid include solvent extraction, supercritical fluid extraction, adsorption elution, and partition chromatography. In addition, green coffee, like roasted coffee, contains abundant caffeine and unsaturated fatty acids.

[0004] Grape seeds contain 14-17% oil, comparable to corn germ, rice bran, and soybeans. Grape seed oil is rich in unsaturated fatty acids, polyphenols, vitamin E, and phytosterols, among other chemical components. Unsaturated fatty acids make up as much as 90% of the oil, with linoleic acid accounting for 66-81.5%. Linoleic acid is an essential fatty acid that participates in the synthesis of phospholipids in cells; the phospholipid bilayer forms the basic framework of the cell membrane. Grape seed oil is also rich in tocopherols and tocotrienols, and its composition is relatively balanced, making it a good source of vitamin E.

[0005] Coconut oil is a natural oil containing various fatty acids with diverse benefits, such as anti-inflammatory and moisturizing properties. It is particularly high in lauric acid, which possesses antibacterial properties and protects the skin. In personal care products, the application of coconut oil in hair care products is currently the most researched area. Studies have shown that the efficacy of coconut oil is closely related to its chemical composition. Furthermore, different extraction methods can affect the fatty acid composition of coconut oil, thereby influencing its physicochemical properties.

[0006] Dark circles under the eyes have become increasingly common in recent years, affecting people of all ages and attracting much attention due to their impact on patients' physical and mental health. However, there is currently limited literature on the diagnosis and treatment of dark circles. Furthermore, with age, aging problems around the eyes are the first to appear. The loss of fat and collagen in the eye area causes sunken eye sockets and sagging eyelids, leading to wrinkles and shadows around the eyes. External aggressors and unhealthy lifestyle habits induce oxidative stress and inflammatory responses, which not only stimulate melanin production but also induce the expression of vascular endothelial growth factor (VEGF). Overexpression of VEGF-A increases capillary permeability, causing red blood cell leakage. This slows blood flow under the eyes, causing stagnation and increased blood leakage, resulting in an unhealthy bluish color around the eyes. This exacerbates the formation of dark circles. The skin around the eyes is the thinnest, which also leads to earlier aging of the eye area compared to other areas of the face, making a person appear tired and aged.

[0007] In existing technologies, in order to improve the skin care effect of oil-soluble ingredients, compounding technology is usually used to mix multiple oil-soluble ingredients in a certain proportion to achieve a synergistic effect.

[0008] However, in existing technologies, most oil-soluble raw materials used in cosmetics are synthetic oils and waxes. While these synthetic raw materials are cheaper, their skincare efficacy is limited, and they pose a risk of irritant residue, potentially burdening the skin with long-term use. Although some existing technologies have attempted to use natural oil extracts as cosmetic raw materials, several problems remain. First, the extraction of natural oils is difficult and easily affected by environmental factors, resulting in poor stability. Second, issues such as residual organic solvents used in the extraction process and the destruction of heat-sensitive active ingredients by high temperatures affect the biosafety and bioavailability of the raw materials. Furthermore, while existing oil blending technologies can improve skincare efficacy, determining the optimal ratio of various oils remains a challenge. Finally, market feedback indicates that most people with dark circles suffer from combination-type dark circles, requiring a comprehensive solution. Existing technologies often offer relatively singular solutions for specific types of eye problems, exhibiting certain limitations.

[0009] Therefore, how to develop a cosmetic composition that has good skin care effects and safe ingredients is a problem that urgently needs to be solved by current technology. Summary of the Invention

[0010] The technical problem this invention aims to solve is to overcome the shortcomings of existing cosmetic oil-soluble raw materials, which either have poor skincare effects or are unsafe in terms of ingredients. This invention provides an oil composition, its preparation method, its application, and skincare products. The oil composition of this invention is safe and has anti-inflammatory, soothing, whitening, and vasodilatory effects, effectively improving dark circles under the eyes.

[0011] The present invention provides an oil composition comprising coffee oil, grape seed oil and coconut oil.

[0012] In a preferred embodiment of the present invention, the mass ratio of coffee oil: grape seed oil: coconut oil is (0.5~2):(0.5~3.5):(3~10), for example (0.5~2):(1~3.5):(3~10), and even more preferably (0.5~1):(1~3.5):(6~10), and more preferably (0.5~1):(1~2):(6~8).

[0013] In a preferred embodiment of the present invention, the mass ratio of coffee oil: grape seed oil: coconut oil is 0.5:3.5:6, 1:1:8, 2:3.5:3, 0.5:1:10, 2:2:6 or 2:0.5:10.

[0014] In this invention, the oil composition has skin care effects. These skin care effects may include, but are not limited to, moisturizing, repairing, soothing, whitening, and reducing dark circles.

[0015] In one embodiment of the present invention, the coffee oil is green coffee oil, also known as raw coffee oil.

[0016] In one embodiment of the present invention, the coffee oil is obtained using a supercritical fluid extraction method, such as a supercritical CO2 extraction method.

[0017] In one embodiment of the present invention, the coffee oil is prepared by the following method:

[0018] It is prepared by freeze-drying, grinding, and supercritical fluid extraction of green coffee beans.

[0019] The green coffee beans mentioned generally refer to the seeds of the small-fruited coffee (Coffea Arabica L.) plant, which are unroasted coffee seeds.

[0020] The freeze-drying process can be carried out in a freeze dryer (e.g., LC-18N-50D).

[0021] The freeze-drying time can be 12~26H, preferably 18~20H, for example 12H, 20H or 26H.

[0022] The pulverization can be pulverized to pass through a 30-60 mesh sieve, preferably a 40-50 mesh sieve, for example a 30-50 mesh sieve.

[0023] The extraction can be carried out in an extraction vessel. The amount of packing material to be extracted in the extraction vessel can be approximately 50-70% of the vessel volume, more preferably 60-65%, for example 50%, 60% or 70%.

[0024] The extraction temperature can be 33~58℃, preferably 45~50℃, for example 33℃, 50℃ or 58℃.

[0025] The extraction pressure can be 15-35 MPa, more preferably 25-30 MPa, for example 15 MPa, 30 MPa or 35 MPa.

[0026] The supercritical fluid may be supercritical CO2. The flow rate of the supercritical CO2 may be 10~30 L / h, more preferably 15~25 L / h, for example 10 L / h or 25 L / h.

[0027] The extraction time can be 1 to 3 hours, for example, 1 hour, 2 hours or 3 hours.

[0028] The extraction process typically includes a separation step. This separation may involve feeding a supercritical fluid carrying the product into a separator, releasing the supercritical fluid under reduced pressure, collecting the oil, and obtaining the coffee oil.

[0029] The pressure of the separator can be 5~10MPa, preferably 5~8MPa, for example 5MPa, 7MPa or 8MPa.

[0030] The oil can be filtered using a 0.5~1.0μm filter membrane, for example, a 0.8μm filter membrane.

[0031] In one embodiment of the present invention, the freeze-drying process in the method for preparing the coffee oil is as follows:

[0032] A1. Take out the frozen fresh green coffee beans and place them directly into a freeze dryer (LC-18N-50D) for freeze drying; then crush the freeze-dried green coffee beans and sieve them.

[0033] Optionally, the freeze-drying time can be 12-26 hours, more preferably 18-20 hours. After pulverization, the product is passed through a 30-60 mesh sieve, most preferably a 40-50 mesh sieve.

[0034] In one embodiment of the present invention, the extraction process in the method for preparing the coffee oil is as follows:

[0035] A2. Place the raw coffee powder obtained in step A1 into the extraction vessel of the supercritical device, and set the extraction temperature on the temperature control panel; after the vessel temperature reaches the set value and stabilizes, start pressurizing to the set value; after the pressure inside the vessel reaches the set value and stabilizes, adjust the opening and closing degree of the exhaust valve to control the flow rate of CO2, and start the extraction process, maintaining constant temperature and pressure in the extraction vessel.

[0036] In one embodiment of the present invention, the separation process after extraction in the method for preparing coffee oil is as follows:

[0037] A3. After extraction, the supercritical fluid carrying the product is fed into the separator. After passing through the pressure reducing valve, it expands and releases raw coffee seed oil, which is then converted into atmospheric pressure CO2 gas. The oil is collected and filtered using a 0.8μm filter membrane to obtain raw coffee seed oil.

[0038] In one specific embodiment of the present invention, the extraction process in the method for preparing coffee oil is as follows:

[0039] The extraction is carried out in an extraction vessel, and the amount of packing material to be extracted in the extraction vessel is 70% of the vessel volume;

[0040] The extraction temperature was 58°C;

[0041] The extraction pressure was 35 MPa;

[0042] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 25 L / h;

[0043] The extraction time was 3 hours.

[0044] In one specific embodiment of the present invention, the extraction process in the method for preparing coffee oil is as follows:

[0045] The extraction is carried out in an extraction vessel, and the amount of packing material to be extracted in the extraction vessel is 60% of the vessel volume;

[0046] The extraction temperature is 50°C;

[0047] The extraction pressure was 30 MPa;

[0048] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 25 L / h;

[0049] The extraction time was 2 hours.

[0050] In one specific embodiment of the present invention, the extraction process in the method for preparing coffee oil is as follows:

[0051] The extraction is carried out in an extraction vessel, and the amount of packing material to be extracted in the extraction vessel is 50% of the vessel volume;

[0052] The extraction temperature was 33°C;

[0053] The extraction pressure was 15 MPa;

[0054] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 10 L / h;

[0055] The extraction time is 1 hour.

[0056] In one specific embodiment of the present invention, the extraction process in the method for preparing coffee oil is as follows:

[0057] The extraction is carried out in an extraction vessel, and the amount of packing material to be extracted in the extraction vessel is 70% of the vessel volume;

[0058] The extraction temperature was 58°C;

[0059] The extraction pressure was 35 MPa;

[0060] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 25 L / h;

[0061] The extraction time was 3 hours.

[0062] In one specific embodiment of the present invention, the extraction process in the method for preparing coffee oil is as follows:

[0063] The extraction is carried out in an extraction vessel, and the amount of packing material to be extracted in the extraction vessel is 50% of the vessel volume;

[0064] The extraction temperature was 33°C;

[0065] The extraction pressure was 15 MPa;

[0066] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 10 L / h;

[0067] The extraction time is 1 hour.

[0068] In one specific embodiment of the present invention, the extraction process in the method for preparing coffee oil is as follows:

[0069] The extraction is carried out in an extraction vessel, and the amount of packing material to be extracted in the extraction vessel is 50% of the vessel volume;

[0070] The extraction temperature was 33°C;

[0071] The extraction pressure was 15 MPa;

[0072] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 10 L / h;

[0073] The extraction time is 1 hour.

[0074] In one embodiment of the present invention, the grape seed oil is obtained using a supercritical fluid extraction method, for example, using a supercritical CO2 extraction method.

[0075] In one embodiment of the present invention, the grape seed oil is obtained by the following method:

[0076] It is prepared by supercritical fluid extraction of grape seed powder.

[0077] The grape seeds mentioned generally refer to the seeds of grapes (Vitis vinifera L.), with a moisture content ranging from 2% to 5%.

[0078] The grape seed powder can be a powder that passes through a 20-50 mesh sieve, preferably a 30-40 mesh sieve, for example, a 20 mesh, 40 mesh, or 50 mesh sieve.

[0079] The extraction can be carried out in an extraction vessel. The amount of packing material to be extracted in the extraction vessel can be approximately 50-70% of the vessel volume, more preferably 60-65%, for example 50%, 65% or 70%.

[0080] The extraction temperature can be 30~60℃, more preferably 45~50℃, for example 30℃, 50℃ or 60℃.

[0081] The extraction pressure can be 20-40 MPa, more preferably 25-30 MPa, for example 20 MPa, 30 MPa or 40 MPa.

[0082] The supercritical fluid may be supercritical CO2. The flow rate of the supercritical CO2 may be 10~30 L / h, more preferably 15~25 L / h, for example 10 L / h, 25 L / h or 30 L / h.

[0083] The extraction time can be 1 to 3 hours, more preferably 1.5 to 2 hours, for example 1 hour, 2 hours or 3 hours.

[0084] The extraction process typically includes a separation step. This separation may involve feeding a supercritical fluid carrying the product into a separator, releasing the supercritical fluid under reduced pressure, collecting the oil, and obtaining the grape seed oil.

[0085] The pressure of the separator can be 5~10MPa, preferably 5~8MPa, for example 5MPa, 7MPa or 8MPa.

[0086] The oil can be filtered using a 0.5~1.0μm filter membrane, for example, a 0.8μm filter membrane.

[0087] In one embodiment of the present invention, the preparation process of the grape seed powder in the method for preparing grape seed oil is as follows:

[0088] B1. Grind the dried grape seeds using a grinder and then sieve them; the sieve should be 20-50 mesh, or more preferably 30-40 mesh.

[0089] In one embodiment of the present invention, the extraction process in the method for preparing grape seed oil is as follows:

[0090] B2. Place the grape seed powder obtained in step B1 into the extraction vessel of the supercritical fluid extraction device, and set the extraction temperature on the temperature control panel. After the vessel temperature reaches the set value and stabilizes, start pressurizing to the set value. After the pressure inside the vessel reaches the set value and stabilizes, adjust the opening and closing degree of the exhaust valve to control the flow rate of CO2 and start the extraction process. Maintain constant temperature and pressure in the extraction vessel.

[0091] In one embodiment of the present invention, the separation process after extraction in the method for preparing grape seed oil is as follows:

[0092] B3. After extraction, the supercritical fluid carrying the product is fed into the separator. After passing through the pressure reducing valve, it expands and releases the raw coffee seed oil, which is then converted into atmospheric pressure CO2 gas. The oil is collected and filtered using a 0.8μm filter membrane to obtain grape seed oil.

[0093] In a specific embodiment of the present invention, the extraction process in the method for preparing grape seed oil is as follows:

[0094] The extraction is carried out in an extraction vessel, and the amount of packing material to be extracted in the extraction vessel is 50% of the vessel volume;

[0095] The extraction temperature is 30°C;

[0096] The extraction pressure was 20 MPa;

[0097] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 10 L / h;

[0098] The extraction time is 1 hour.

[0099] In a specific embodiment of the present invention, the extraction process in the method for preparing grape seed oil is as follows:

[0100] The extraction is carried out in an extraction vessel, and the amount of packing material to be extracted in the extraction vessel is 70% of the vessel volume;

[0101] The extraction temperature is 60°C;

[0102] The extraction pressure was 40 MPa;

[0103] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 30 L / h;

[0104] The extraction time was 3 hours.

[0105] In a specific embodiment of the present invention, the extraction process in the method for preparing grape seed oil is as follows:

[0106] The extraction is carried out in an extraction vessel, and the amount of packing material to be extracted in the extraction vessel is 50% of the vessel volume;

[0107] The extraction temperature is 30°C;

[0108] The extraction pressure was 20 MPa;

[0109] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 10 L / h;

[0110] The extraction time is 1 hour.

[0111] In a specific embodiment of the present invention, the extraction process in the method for preparing grape seed oil is as follows:

[0112] The extraction is carried out in an extraction vessel, and the amount of packing material to be extracted in the extraction vessel is 70% of the vessel volume;

[0113] The extraction temperature is 60°C;

[0114] The extraction pressure was 40 MPa;

[0115] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 30 L / h;

[0116] The extraction time was 3 hours.

[0117] In a specific embodiment of the present invention, the extraction process in the method for preparing grape seed oil is as follows:

[0118] The extraction is carried out in an extraction vessel, wherein the amount of packing material to be extracted in the extraction vessel is 65% of the vessel volume;

[0119] The extraction temperature is 50°C;

[0120] The extraction pressure was 30 MPa;

[0121] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 25 L / h;

[0122] The extraction time was 2 hours.

[0123] In a specific embodiment of the present invention, the extraction process in the method for preparing grape seed oil is as follows:

[0124] The extraction is carried out in an extraction vessel, and the amount of packing material to be extracted in the extraction vessel is 70% of the vessel volume;

[0125] The extraction temperature is 60°C;

[0126] The extraction pressure was 40 MPa;

[0127] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 30 L / h;

[0128] The extraction time was 3 hours.

[0129] In one embodiment of the present invention, the coconut oil is obtained using a supercritical fluid extraction method, for example, using a supercritical CO2 extraction method.

[0130] In one embodiment of the present invention, the coconut oil is prepared by the following method:

[0131] The product is prepared by supercritical fluid extraction of coconut meat powder.

[0132] The coconut flesh generally refers to the endosperm of the coconut (Cocos nucifera L.), also known as the coconut pulp.

[0133] The coconut meat powder can be a powder that passes through a 30-60 mesh sieve, preferably a 40-50 mesh sieve, for example, a 30 mesh, 40 mesh, 50 mesh or 60 mesh sieve.

[0134] The extraction can be carried out in an extraction vessel. The amount of packing material to be extracted in the extraction vessel can be approximately 50-70% of the vessel volume, more preferably 60-65%, for example 50%, 60%, 65% or 70%.

[0135] The extraction temperature can be 30~50℃, more preferably 40~45℃, for example 30℃, 40℃, 45℃ or 50℃.

[0136] The extraction pressure can be 20-40 MPa, more preferably 25-30 MPa, for example 20 MPa, 25 MPa, 30 MPa or 40 MPa.

[0137] The supercritical fluid may be supercritical CO2. The flow rate of the supercritical CO2 may be 10~30 L / h, more preferably 15~25 L / h, for example 10 L / h, 15 L / h, 25 L / h or 30 L / h.

[0138] The extraction time can be 0.5 to 2 hours, more preferably 1 to 1.5 hours, for example 0.5 hours, 1 hour, 1.5 hours or 2 hours.

[0139] The extraction process typically includes a separation step. This separation may involve feeding a supercritical fluid carrying the product into a separator, releasing the supercritical fluid under reduced pressure, collecting the oil, and obtaining the coconut oil.

[0140] The pressure of the separator can be 5~10MPa, preferably 5~8MPa, for example 5MPa, 6MPa, 7MPa or 8MPa.

[0141] The oil can be filtered using a 0.5~1.0μm filter membrane, for example, a 0.8μm filter membrane.

[0142] In one embodiment of the present invention, the preparation process of the coconut pulp powder in the method for preparing coconut oil is as follows:

[0143] C1. Take out the frozen coconut meat, slice it, dry and crush it, and then sieve it;

[0144] After further pulverization, it is passed through a 30-60 mesh sieve, with the most preferred option being a 40-50 mesh sieve.

[0145] In one embodiment of the present invention, the extraction process in the method for preparing coconut oil is as follows:

[0146] C2. Place the coconut powder obtained in step C1 into the extraction vessel of the supercritical fluid extraction device, and set the extraction temperature on the temperature control panel. After the vessel temperature reaches the set value and stabilizes, begin pressurizing to the set value. After the pressure inside the vessel reaches the set value and stabilizes, adjust the opening degree of the exhaust valve to control the CO2 flow rate and start the extraction process, maintaining constant temperature and pressure in the extraction vessel.

[0147] C3. After extraction, the supercritical fluid carrying the product is fed into a separator. After passing through a pressure reducing valve, it expands and releases raw coconut oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain coconut oil.

[0148] In a specific embodiment of the present invention, the extraction process in the method for preparing coconut oil is as follows:

[0149] The extraction is carried out in an extraction vessel, and the amount of packing material to be extracted in the extraction vessel is 60% of the vessel volume;

[0150] The extraction temperature was 40°C;

[0151] The extraction pressure was 25 MPa;

[0152] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 15 L / h;

[0153] The extraction time is 1 hour.

[0154] In a specific embodiment of the present invention, the extraction process in the method for preparing coconut oil is as follows:

[0155] The extraction is carried out in an extraction vessel, wherein the amount of packing material to be extracted in the extraction vessel is 65% of the vessel volume;

[0156] The extraction temperature was 45°C;

[0157] The extraction pressure was 30 MPa;

[0158] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 25 L / h;

[0159] The extraction time was 1.5 hours.

[0160] In a specific embodiment of the present invention, the extraction process in the method for preparing coconut oil is as follows:

[0161] The extraction is carried out in an extraction vessel, and the amount of packing material to be extracted in the extraction vessel is 70% of the vessel volume;

[0162] The extraction temperature is 50°C;

[0163] The extraction pressure was 40 MPa;

[0164] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 30 L / h;

[0165] The extraction time was 2 hours.

[0166] In a specific embodiment of the present invention, the extraction process in the method for preparing coconut oil is as follows:

[0167] The extraction is carried out in an extraction vessel, and the amount of packing material to be extracted in the extraction vessel is 50% of the vessel volume;

[0168] The extraction temperature is 30°C;

[0169] The extraction pressure was 20 MPa;

[0170] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 10 L / h;

[0171] The extraction time was 0.5 hours.

[0172] In a specific embodiment of the present invention, the extraction process in the method for preparing coconut oil is as follows:

[0173] The extraction is carried out in an extraction vessel, and the amount of packing material to be extracted in the extraction vessel is 60% of the vessel volume;

[0174] The extraction temperature was 40°C;

[0175] The extraction pressure was 25 MPa;

[0176] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 15 L / h;

[0177] The extraction time is 1 hour.

[0178] In a specific embodiment of the present invention, the extraction process in the method for preparing coconut oil is as follows:

[0179] The extraction is carried out in an extraction vessel, and the amount of packing material to be extracted in the extraction vessel is 50% of the vessel volume;

[0180] The extraction temperature is 30°C;

[0181] The extraction pressure was 20 MPa;

[0182] The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 is 10 L / h;

[0183] The extraction time was 0.5 hours.

[0184] The present invention also provides a method for preparing the oil composition, wherein the coffee oil, the grape seed oil and the coconut oil are mixed together.

[0185] The present invention also provides an application of the oil composition as an oil-soluble ingredient in skin care products.

[0186] In this invention, the skin care products may include, but are not limited to, face creams, essential oils, lotions, and facial cleansers.

[0187] The present invention also provides a skin care product comprising the oil composition.

[0188] In one embodiment of the present invention, the oil composition in the skin care product has a mass percentage of 1 to 10%, for example 5%.

[0189] In one embodiment of the present invention, the skincare product comprises 5% of the natural oil composition and 95% ethylhexyl nonanoate; the percentage refers to the mass percentage. The ethylhexyl nonanoate may be ethylhexyl nonanoate derived from sunflower seeds.

[0190] Based on common knowledge in the field, the above-mentioned preferred conditions can be combined arbitrarily to obtain various preferred embodiments of the present invention.

[0191] The reagents and raw materials used in this invention are all commercially available.

[0192] The positive and progressive effects of this invention are as follows:

[0193] Compared with existing oil compositions, the oil compositions of the present invention have the following advantages:

[0194] 1. Improved bioavailability: This invention uses supercritical fluid extraction to extract green coffee oil, grape seed oil, and coconut oil. These natural oils have high bioavailability and can be effectively absorbed by the skin, thereby improving skincare effects. Compared to synthetic oils and waxes and naturally derived oil extracts, the bioavailability of this invention is significantly improved.

[0195] 2. Solves the problem of solvent residue and improves the quality of vegetable oils: Traditional extraction methods often require the use of large amounts of solvent, which can easily lead to solvent residue and affect the safety and quality of the product. The extraction process of this invention uses a supercritical method, which does not require the use of any solvent, thus avoiding the problem of solvent residue in traditional synthetic oils and waxes and natural oil extracts. This is a significant advantage compared to existing technologies, improving the safety and quality of the product. At the same time, this invention also solves the problem of low yield of natural oil extracts, improves the efficiency of raw material utilization, reduces production costs, and meets the market demand for efficient, environmentally friendly, and economical extraction technologies.

[0196] 3. Multiple Benefits: The oil composition of this invention not only addresses vascular and pigmented dark circles, but also provides excellent moisturizing and skin-care effects. Compared to existing technologies, this offers a wider range of applications and superior skincare efficacy, meeting the market's demand for highly effective, safe, and multifunctional skincare products.

[0197] 4. Clinical Validation: The composition of this invention has undergone clinical testing and validation, and its significant efficacy has been confirmed. This demonstrates higher credibility and reliability compared to existing technologies.

[0198] In summary, compared with existing technologies, this invention offers higher bioavailability, better safety, lower cost, wider application range, and greater reliability, making it a cosmetic ingredient with superior skincare efficacy. Therefore, the oil composition of this invention has broad applications in the fields of cosmetic technology, natural product extraction technology, and chemical engineering technology. Attached Figure Description

[0199] Figure 1 This is a comparison chart of the relative VEGF expression levels in each group in Example 3.

[0200] Figure 2This is a comparison chart of the average change rate of L* values ​​after 14 days and 28 days of use in the test product and placebo groups in Example 4.

[0201] Figure 3 This is a comparison chart of the average change rate of a* values ​​after 14 days and 28 days of use in the test product and placebo groups in Example 4.

[0202] Figure 4 This is a comparison chart of the average change rate of b* values ​​after 14 days and 28 days of use in the test product and placebo groups in Example 4.

[0203] Figure 5 The graph shows a comparison of the average change rate of melanin content in the test product and placebo groups after 14 days and 28 days of use, as shown in Example 4.

[0204] Figure 6 The comparison of eye images before and 28 days after using the test product in Example 4 is shown. Detailed Implementation

[0205] The present invention is further illustrated below by way of embodiments, but the invention is not limited to the scope of the embodiments described herein. Experimental methods in the following embodiments that do not specify specific conditions were performed according to conventional methods and conditions, or as selected according to the product instructions.

[0206] In the following embodiments:

[0207] The plant source of green coffee beans is the seeds of Coffea Arabica L., a plant in the Rubiaceae family;

[0208] The plant source of grape seeds is the seed of grape (Vitis vinifera L.), with a moisture content in the range of 2%-5%;

[0209] The plant source of coconut flesh is the endosperm of the coconut (Cocos nucifera L.), also known as coconut pulp.

[0210] Example 1

[0211] (1) This embodiment provides a natural oil composition with skin care effects. The natural oil composition is coffee oil, grape seed oil and coconut oil. The green coffee oil, grape seed oil and coconut oil are mixed evenly in a mass ratio of 0.5:3.5:6.

[0212] (2) This embodiment also provides a method for preparing a natural oil composition with skin care effects. The method involves extracting raw coffee seed oil, grape seed oil and coconut oil using a supercritical CO2 method, and then mixing them evenly according to the mass ratio in (1) to obtain a natural oil composition.

[0213] The preparation method of raw coffee seed oil in the natural oil composition in this embodiment is as follows:

[0214] A1. Take the frozen fresh coffee beans out and place them directly into a freeze dryer (LC-18N-50D) for 26 hours of freeze-drying. Grind the freeze-dried coffee beans and then pass them through a 50-mesh sieve.

[0215] A2. Place the raw coffee powder obtained in step A1 into the extraction vessel of the supercritical fluid extraction device. The packing material (referring to the raw coffee powder obtained in A1) should fill approximately 70% of the vessel's volume. Set the extraction temperature to 58°C on the temperature control panel. After the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 35 MPa. Once the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening and closing of the exhaust valve to control the CO2 flow rate at 25 L / h, and begin the extraction process. Maintain constant temperature and pressure in the extraction vessel and extract for 3 hours.

[0216] A3. After extraction, the supercritical fluid carrying the product is fed into a separator. The separator pressure is set to 8 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coffee seed oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain raw coffee seed oil.

[0217] The method for preparing grape seed oil in the natural oil composition in this embodiment is as follows:

[0218] B1. Purchase dried grape seeds, grind them using a grinder, and then pass them through a 20-mesh sieve.

[0219] B2. Place the grape seed powder obtained in step B1 into the extraction vessel of the supercritical fluid extraction device, with the material volume approximately 50% of the vessel's volume. Set the extraction temperature to 30°C on the temperature control panel. After the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 20 MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening and closing degree of the exhaust valve to control the CO2 flow rate at 10 L / h, and begin the extraction process. Maintain constant temperature and pressure in the extraction vessel and extract for 1 hour.

[0220] B3. After extraction, the supercritical fluid carrying the product is fed into a separator. The separator pressure is set to 5 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coffee seed oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain grape seed oil.

[0221] The method for preparing coconut oil in the natural oil composition in this embodiment is as follows:

[0222] C1. Take out the frozen coconut meat, slice it, dry and crush it, and then pass it through a 40-mesh sieve.

[0223] C2. Place the coconut powder obtained in step S1 into the extraction vessel of the supercritical fluid extraction device, with the material volume approximately 60% of the vessel's volume. Set the extraction temperature to 40℃ on the temperature control panel. After the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 25MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening and closing degree of the exhaust valve to control the CO2 flow rate at 15L / h, and begin the extraction process. Maintain constant temperature and pressure in the extraction vessel. Extract for 1 hour.

[0224] C3. After extraction, the supercritical fluid carrying the product is fed into a separator, with the separator pressure set to 6 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coconut oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain coconut oil.

[0225] Table 1

[0226]

[0227] Example 2

[0228] (1) This embodiment provides a natural oil composition with skin care effects. The natural oil composition is coffee oil, grape seed oil and coconut oil. The green coffee oil, grape seed oil and coconut oil are mixed evenly in a mass ratio of 1:1:8.

[0229] (2) This embodiment also provides a method for preparing a natural oil composition with skin care effects. The method involves extracting raw coffee seed oil, grape seed oil and coconut oil using a supercritical CO2 method, and then mixing them evenly according to the mass ratio in (1) to obtain a natural oil composition.

[0230] The preparation method of raw coffee seed oil in the natural oil composition in this embodiment is as follows:

[0231] A1. Take the frozen fresh coffee beans out and place them directly into a freeze dryer (LC-18N-50D) for 20 hours of freeze-drying. Crush the freeze-dried coffee beans and then pass them through a 50-mesh sieve.

[0232] A2. Place the raw coffee powder obtained in step A1 into the extraction vessel of the supercritical fluid extraction device, filling the vessel to approximately 60% of its volume. Set the extraction temperature to 50°C on the temperature control panel. Once the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 30 MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening of the exhaust valve to control the CO2 flow rate at 25 L / h, and begin the extraction process. Maintain constant temperature and pressure within the extraction vessel and extract for 2 hours.

[0233] A3. After extraction, the supercritical fluid carrying the product is fed into a separator. The separator pressure is set to 7 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coffee seed oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain raw coffee seed oil.

[0234] The method for preparing grape seed oil in the natural oil composition in this embodiment is as follows:

[0235] B1. Purchase dried grape seeds, grind them using a grinder, and then pass them through a 50-mesh sieve.

[0236] B2. Place the grape seed powder obtained in step B1 into the extraction vessel of the supercritical fluid extraction device, filling it to approximately 70% of the vessel's volume. Set the extraction temperature to 60°C on the temperature control panel. Once the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 40 MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening and closing of the exhaust valve to control the CO2 flow rate at 30 L / h, and begin the extraction process. Maintain constant temperature and pressure within the extraction vessel and extract for 3 hours.

[0237] B3. After extraction, the supercritical fluid carrying the product is fed into a separator. The separator pressure is set to 8 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coffee seed oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain grape seed oil.

[0238] The method for preparing coconut oil in the natural oil composition in this embodiment is as follows:

[0239] C1. Take out the frozen coconut meat, slice it, dry and crush it, and then pass it through a 50-mesh sieve.

[0240] C2. Place the coconut powder obtained in step S1 into the extraction vessel of the supercritical fluid extraction device, with the material volume approximately 65% ​​of the vessel's volume. Set the extraction temperature to 45℃ on the temperature control panel. After the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 30MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening and closing degree of the exhaust valve to control the CO2 flow rate at 25L / h, and begin the extraction process. Maintain constant temperature and pressure in the extraction vessel. Extract for 1.5 hours.

[0241] C3. After extraction, the supercritical fluid carrying the product is fed into a separator, with the separator pressure set to 7 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coconut oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain coconut oil.

[0242] Table 2

[0243]

[0244] Example 3

[0245] (1) This embodiment provides a natural oil composition with skin care effects. The natural oil composition is coffee oil, grape seed oil and coconut oil. The green coffee oil, grape seed oil and coconut oil are mixed evenly in a mass ratio of 2:3.5:3.

[0246] (2) This embodiment also provides a method for preparing a natural oil composition with skin care effects, wherein coffee seed oil, grape seed oil and coconut oil are extracted by supercritical CO2 method, and then mixed evenly according to the mass ratio in (1) to obtain the natural oil composition.

[0247] The preparation method of raw coffee seed oil in the natural oil composition in this embodiment is as follows:

[0248] A1. Take the frozen fresh coffee beans out and place them directly into a freeze dryer (LC-18N-50D) for 12 hours of freeze-drying. Grind the freeze-dried coffee beans and then pass them through a 30-mesh sieve.

[0249] A2. Place the raw coffee powder obtained in step A1 into the extraction vessel of the supercritical fluid extraction device, filling the vessel to approximately 50% of its volume. Set the extraction temperature to 33°C on the temperature control panel. Once the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 15 MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening of the exhaust valve to control the CO2 flow rate at 10 L / h, and begin the extraction process. Maintain constant temperature and pressure within the extraction vessel and extract for 1 hour.

[0250] A3. After extraction, the supercritical fluid carrying the product is fed into a separator. The separator pressure is set to 5 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coffee seed oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain raw coffee seed oil.

[0251] The method for preparing grape seed oil in the natural oil composition in this embodiment is as follows:

[0252] B1. Purchase dried grape seeds, grind them using a grinder, and then pass them through a 20-mesh sieve.

[0253] B2. Place the grape seed powder obtained in step B1 into the extraction vessel of the supercritical fluid extraction device, with the material volume approximately 50% of the vessel's volume. Set the extraction temperature to 30°C on the temperature control panel. After the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 20 MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening and closing degree of the exhaust valve to control the CO2 flow rate at 10 L / h, and begin the extraction process. Maintain constant temperature and pressure in the extraction vessel and extract for 1 hour.

[0254] B3. After extraction, the supercritical fluid carrying the product is fed into a separator. The separator pressure is set to 5 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coffee seed oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain grape seed oil.

[0255] The method for preparing coconut oil in the natural oil composition in this embodiment is as follows:

[0256] C1. Take out the frozen coconut meat, slice it, dry and crush it, and then pass it through a 60-mesh sieve.

[0257] C2. Place the coconut powder obtained in step S1 into the extraction vessel of the supercritical fluid extraction device, filling approximately 70% of the vessel's volume. Set the extraction temperature to 50°C on the temperature control panel. Once the vessel temperature reaches and stabilizes, begin pressurizing to the set value of 40 MPa. After the pressure inside the vessel reaches and stabilizes, adjust the opening of the exhaust valve to control the CO2 flow rate at 30 L / h, and begin the extraction process. Maintain constant temperature and pressure within the extraction vessel. Extract for 2 hours.

[0258] C3. After extraction, the supercritical fluid carrying the product is fed into a separator, with the separator pressure set to 8 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coconut oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain coconut oil.

[0259] Table 3

[0260]

[0261] Example 4

[0262] (1) This embodiment provides a natural oil composition with skin care effects. The natural oil composition is coffee oil, grape seed oil and coconut oil. The green coffee oil, grape seed oil and coconut oil are mixed evenly in a mass ratio of 0.5:1:10.

[0263] (2) This embodiment also provides a method for preparing a natural oil composition with skin care effects, wherein coffee seed oil, grape seed oil and coconut oil are extracted by supercritical CO2 method, and then mixed evenly according to the mass ratio in (1) to obtain the natural oil composition.

[0264] The preparation method of raw coffee seed oil in the natural oil composition in this embodiment is as follows:

[0265] A1. Take the frozen fresh coffee beans out and place them directly into a freeze dryer (LC-18N-50D) for 26 hours of freeze-drying. Grind the freeze-dried coffee beans and then pass them through a 50-mesh sieve.

[0266] A2. Place the raw coffee powder obtained in step A1 into the extraction vessel of the supercritical fluid extraction device, filling the vessel to approximately 70% of its volume. Set the extraction temperature to 58°C on the temperature control panel. Once the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 35 MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening of the exhaust valve to control the CO2 flow rate at 25 L / h, and begin the extraction process. Maintain constant temperature and pressure within the extraction vessel and extract for 3 hours.

[0267] A3. After extraction, the supercritical fluid carrying the product is fed into a separator. The separator pressure is set to 8 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coffee seed oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain raw coffee seed oil.

[0268] The method for preparing grape seed oil in the natural oil composition in this embodiment is as follows:

[0269] B1. Purchase dried grape seeds, grind them using a grinder, and then pass them through a 50-mesh sieve.

[0270] B2. Place the grape seed powder obtained in step B1 into the extraction vessel of the supercritical fluid extraction device, filling it to approximately 70% of the vessel's volume. Set the extraction temperature to 60°C on the temperature control panel. Once the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 40 MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening and closing of the exhaust valve to control the CO2 flow rate at 30 L / h, and begin the extraction process. Maintain constant temperature and pressure within the extraction vessel and extract for 3 hours.

[0271] B3. After extraction, the supercritical fluid carrying the product is fed into a separator. The separator pressure is set to 8 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coffee seed oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain grape seed oil.

[0272] The method for preparing coconut oil in the natural oil composition in this embodiment is as follows:

[0273] C1. Take out the frozen coconut meat, slice it, dry and crush it, and then pass it through a 30-mesh sieve.

[0274] C2. Place the coconut powder obtained in step S1 into the extraction vessel of the supercritical fluid extraction device, with the material volume approximately 50% of the vessel's volume. Set the extraction temperature to 30℃ on the temperature control panel. After the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 20MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening and closing degree of the exhaust valve to control the CO2 flow rate at 10L / h, and begin the extraction process. Maintain constant temperature and pressure in the extraction vessel. Extract for 0.5 hours.

[0275] C3. After extraction, the supercritical fluid carrying the product is fed into a separator, with the separator pressure set to 5 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coconut oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain coconut oil.

[0276] Table 4

[0277]

[0278] Example 5

[0279] (1) This embodiment provides a natural oil composition with skin care effects. The natural oil composition is coffee oil, grape seed oil and coconut oil. The green coffee oil, grape seed oil and coconut oil are mixed evenly in a mass ratio of 2:2:6.

[0280] (2) This embodiment also provides a method for preparing a natural oil composition with skin care effects, wherein coffee seed oil, grape seed oil and coconut oil are extracted by supercritical CO2 method, and then mixed evenly according to the mass ratio in (1) to obtain the natural oil composition.

[0281] The preparation method of raw coffee seed oil in the natural oil composition in this embodiment is as follows:

[0282] A1. Take the frozen fresh coffee beans out and place them directly into a freeze dryer (LC-18N-50D) for 12 hours of freeze-drying. Grind the freeze-dried coffee beans and then pass them through a 30-mesh sieve.

[0283] A2. Place the raw coffee powder obtained in step A1 into the extraction vessel of the supercritical fluid extraction device, filling the vessel to approximately 50% of its volume. Set the extraction temperature to 33°C on the temperature control panel. Once the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 15 MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening of the exhaust valve to control the CO2 flow rate at 10 L / h, and begin the extraction process. Maintain constant temperature and pressure within the extraction vessel and extract for 1 hour.

[0284] A3. After extraction, the supercritical fluid carrying the product is fed into a separator. The separator pressure is set to 5 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coffee seed oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain raw coffee seed oil.

[0285] The method for preparing grape seed oil in the natural oil composition in this embodiment is as follows:

[0286] B1. Purchase dried grape seeds, grind them using a grinder, and then pass them through a 40-mesh sieve.

[0287] B2. Place the grape seed powder obtained in step B1 into the extraction vessel of the supercritical fluid extraction device, with the material volume approximately 65% ​​of the vessel's volume. Set the extraction temperature to 50°C on the temperature control panel. After the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 30 MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening and closing degree of the exhaust valve to control the CO2 flow rate at 25 L / h, and begin the extraction process. Maintain constant temperature and pressure in the extraction vessel and extract for 2 hours.

[0288] B3. After extraction, the supercritical fluid carrying the product is fed into a separator. The separator pressure is set to 7 MPa. After passing through a pressure reducing valve, the fluid expands and releases the raw coffee seed oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain grape seed oil.

[0289] The method for preparing coconut oil in the natural oil composition in this embodiment is as follows:

[0290] C1. Take out the frozen coconut meat, slice it, dry and crush it, and then pass it through a 40-mesh sieve.

[0291] C2. Place the coconut powder obtained in step S1 into the extraction vessel of the supercritical fluid extraction device, with the material volume approximately 60% of the vessel's volume. Set the extraction temperature to 40℃ on the temperature control panel. After the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 25MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening and closing degree of the exhaust valve to control the CO2 flow rate at 15L / h, and begin the extraction process. Maintain constant temperature and pressure in the extraction vessel. Extract for 1 hour.

[0292] C3. After extraction, the supercritical fluid carrying the product is fed into a separator, with the separator pressure set to 6 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coconut oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain coconut oil.

[0293] Table 5

[0294]

[0295] Example 6

[0296] (1) This embodiment provides a natural oil composition with skin care effects, characterized in that the natural oil composition is coffee oil, grape seed oil and coconut oil, and green coffee oil: grape seed oil: coconut oil are mixed evenly in a mass ratio of 2:0.5:10.

[0297] (2) This embodiment also provides a method for preparing a natural oil composition with skin care effects, characterized in that the raw coffee seed oil, grape seed oil and coconut oil are extracted by supercritical CO2 method, and then mixed evenly according to the mass ratio in (1) to obtain the natural oil composition.

[0298] The preparation method of raw coffee seed oil in the natural oil composition in this embodiment is as follows:

[0299] A1. Take the frozen fresh coffee beans out and place them directly into a freeze dryer (LC-18N-50D) for 12 hours of freeze-drying. Grind the freeze-dried coffee beans and then pass them through a 30-mesh sieve.

[0300] A2. Place the raw coffee powder obtained in step A1 into the extraction vessel of the supercritical fluid extraction device, filling the vessel to approximately 50% of its volume. Set the extraction temperature to 33°C on the temperature control panel. Once the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 15 MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening of the exhaust valve to control the CO2 flow rate at 10 L / h, and begin the extraction process. Maintain constant temperature and pressure within the extraction vessel and extract for 1 hour.

[0301] A3. After extraction, the supercritical fluid carrying the product is fed into a separator. The separator pressure is set to 5 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coffee seed oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain raw coffee seed oil.

[0302] The method for preparing grape seed oil in the natural oil composition in this embodiment is as follows:

[0303] B1. Purchase dried grape seeds, grind them using a grinder, and then pass them through a 50-mesh sieve.

[0304] B2. Place the grape seed powder obtained in step B1 into the extraction vessel of the supercritical fluid extraction device, filling it to approximately 70% of the vessel's volume. Set the extraction temperature to 60°C on the temperature control panel. Once the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 40 MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening and closing of the exhaust valve to control the CO2 flow rate at 30 L / h, and begin the extraction process. Maintain constant temperature and pressure within the extraction vessel and extract for 3 hours.

[0305] B3. After extraction, the supercritical fluid carrying the product is fed into a separator. The separator pressure is set to 8 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coffee seed oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain grape seed oil.

[0306] The method for preparing coconut oil in the natural oil composition in this embodiment is as follows:

[0307] C1. Take out the frozen coconut meat, slice it, dry and crush it, and then pass it through a 30-mesh sieve.

[0308] C2. Place the coconut powder obtained in step S1 into the extraction vessel of the supercritical fluid extraction device, with the material volume approximately 50% of the vessel's volume. Set the extraction temperature to 30℃ on the temperature control panel. After the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 20MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening and closing degree of the exhaust valve to control the CO2 flow rate at 10L / h, and begin the extraction process. Maintain constant temperature and pressure in the extraction vessel. Extract for 0.5 hours.

[0309] C3. After extraction, the supercritical fluid carrying the product is fed into a separator, with the separator pressure set to 5 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coconut oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain coconut oil.

[0310] Table 6

[0311]

[0312] Comparative Example 1

[0313] The preparation method of raw coffee seed oil is as follows:

[0314] A1. Take the frozen fresh coffee beans out and place them directly into a freeze dryer (LC-18N-50D) for 26 hours of freeze-drying. Grind the freeze-dried coffee beans and then pass them through a 50-mesh sieve.

[0315] A2. Place the raw coffee powder obtained in step A1 into the extraction vessel of the supercritical fluid extraction device, filling the vessel to approximately 70% of its volume. Set the extraction temperature to 58°C on the temperature control panel. Once the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 35 MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening of the exhaust valve to control the CO2 flow rate at 25 L / h, and begin the extraction process. Maintain constant temperature and pressure within the extraction vessel and extract for 3 hours.

[0316] A3. After extraction, the supercritical fluid carrying the product is fed into a separator. The separator pressure is set to 8 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coffee seed oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain raw coffee seed oil.

[0317] Comparative Example 2

[0318] The preparation method of grape seed oil is as follows:

[0319] B1. Purchase dried grape seeds, grind them using a grinder, and then pass them through a 50-mesh sieve.

[0320] B2. Place the grape seed powder obtained in step B1 into the extraction vessel of the supercritical fluid extraction device, filling it to approximately 70% of the vessel's volume. Set the extraction temperature to 60°C on the temperature control panel. Once the vessel temperature reaches and stabilizes at the set value, begin pressurizing to the set value of 40 MPa. After the pressure inside the vessel reaches and stabilizes at the set value, adjust the opening and closing of the exhaust valve to control the CO2 flow rate at 30 L / h, and begin the extraction process. Maintain constant temperature and pressure within the extraction vessel and extract for 3 hours.

[0321] B3. After extraction, the supercritical fluid carrying the product is fed into a separator. The separator pressure is set to 8 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coffee seed oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain grape seed oil.

[0322] Comparative Example 3

[0323] The preparation method of coconut oil is as follows:

[0324] C1. Take out the frozen coconut meat, slice it, dry and crush it, and then pass it through a 60-mesh sieve.

[0325] C2. Place the coconut powder obtained in step S1 into the extraction vessel of the supercritical fluid extraction device, filling approximately 70% of the vessel's volume. Set the extraction temperature to 50°C on the temperature control panel. Once the vessel temperature reaches and stabilizes, begin pressurizing to the set value of 40 MPa. After the pressure inside the vessel reaches and stabilizes, adjust the opening of the exhaust valve to control the CO2 flow rate at 30 L / h, and begin the extraction process. Maintain constant temperature and pressure within the extraction vessel. Extract for 2 hours.

[0326] C3. After extraction, the supercritical fluid carrying the product is fed into a separator, with the separator pressure set to 8 MPa. After passing through a pressure reducing valve, the fluid expands and releases raw coconut oil, which is then converted into atmospheric pressure CO2 gas. The collected oil is filtered through a 0.8 μm filter membrane to obtain coconut oil.

[0327] Comparative Example 4

[0328] This comparative example provides a natural oil composition with skin care effects. The natural oil composition is sunflower seed oil, apple seed oil, and babassu seed oil. The sunflower seed oil, apple seed oil, and babassu seed oil are uniformly mixed in a mass ratio of sunflower seed oil: apple seed oil: babassu seed oil = 2:1:4.

[0329] The raw materials come from the following sources:

[0330] Sunflower seed oil (rich in chlorogenic acid) is extracted using organic solvents and sourced from Huilang Company.

[0331] Apple seed oil (rich in unsaturated fatty acids) is extracted using supercritical CO2 and sourced from NEOCHEM KOREA, South Korea.

[0332] Babassu seed oil (rich in lauric acid) is cold-pressed and refined, sourced from Greentech, France.

[0333] Table 7

[0334]

[0335] Example 1: Anti-inflammatory and soothing effect test

[0336] The skin under the eyes is thin (four times thinner than other facial skin), making it extremely sensitive to external aggressors (UV rays, smog, and pollution, etc.) and unhealthy lifestyle habits. With age, aging problems around the eyes are the first to appear. Loss of fat and collagen in the eye area causes sunken eye sockets and sagging eyelids, leading to wrinkles and shadows around the eyes. External aggressors and unhealthy lifestyle habits induce oxidative stress and inflammatory responses, not only stimulating melanin production but also inducing the expression of vascular endothelial growth factor (VEGF). Overexpression of VEGF-A increases capillary permeability, causing red blood cell leakage. Blood flow under the eyes slows down, causing stagnation and giving the area an unhealthy bluish-purple color, exacerbating dark circles. The skin around the eyes is the thinnest, which also contributes to premature aging around the eyes compared to other areas of the face, making a person appear tired and aged.

[0337] (1) Experimental method: In this test, macrophages were stimulated with 1 μg / mL lipopolysaccharide (LPS). The anti-inflammatory and soothing effects of the test substance were determined by the inhibition of IL-6 inflammatory factor in LPS-induced Raw264.7 cells by the test sample (0.15%, mass percentage).

[0338] Table 8 Test Plan

[0339]

[0340] (2) Experimental results:

[0341] Table 9

[0342]

[0343] Note: When performing statistical analysis using the t-test method, significance compared with the BC group is indicated by #, with P.value < 0.001 indicated by ###; significance compared with the NC group is indicated by *, with P-value < 0.05 indicated by *, P-value < 0.01 indicated by **, and P.value < 0.001 indicated by ***.

[0344] (3) Experimental conclusions:

[0345] 1) Compared with the BC group, the IL-6 level in the NC group was significantly increased, indicating that the stimulation conditions in this experiment were effective. Compared with the NC group, the IL-6 level in the PC (dexamethasone) group was significantly decreased, indicating that the positive control in this experiment was effective.

[0346] 2) Compared with the NC group, the anti-inflammatory and soothing effects of the sample groups were as follows: Example 4 > Example 1 > Example 2 > Example 5 > Example 6 > Example 3 > Comparative Example 1 > Comparative Example 3 > Comparative Example 4 > Comparative Example 2. Among them, Comparative Examples 1 to 4 showed no significant difference.

[0347] Example 2: Whitening Effect Test

[0348] (1) Experimental method: In this test, 1 μM α-MSH was used to stimulate B16 mouse melanoma cells to produce a large amount of melanin. The whitening effect of the test substance was judged by the inhibition of melanin in α-MSH-induced B16 cells by the test sample (0.5%, mass percentage).

[0349] Table 10 Test Plan

[0350]

[0351] (2) Experimental results:

[0352] Table 11

[0353]

[0354] Note: When performing statistical analysis using the t-test method, significance compared with the NC group is indicated by #, P-value < 0.05 is indicated by #, P-value < 0.01 is indicated by ##, and P-value < 0.001 is indicated by ###.

[0355] (3) Experimental conclusions:

[0356] 1) Compared with the NC group, the relative content of melanin synthesis in the PC (arbutin 100ppm) group was significantly reduced, indicating that the positive control in this experiment was effective.

[0357] 2) Compared with the NC group, the whitening effect of the sample groups was as follows: Example 1 > Example 4 > Example 2 > Example 3 > Example 5 > Example 6 > Comparative Example 2 > Comparative Example 1 > Comparative Example 4 > Comparative Example 3. Among them, Comparative Examples 1 to 4 showed no significant difference.

[0358] Example 3: Test of effect in inhibiting vasodilation

[0359] (1) Experimental Method: In this test, human immortalized keratinocytes (HaCat) were irradiated with 30 mJ / cm² UV light to induce VEGF expression. The inhibitory effect of the test substance on vasodilation and angiogenesis was determined by the inhibition of VEGF expression in UV-induced keratinocytes by the test sample (1.25%). Preferably, the test substances from Examples 1 and 4 were used as test substances. Suramin (CAS No.: 145-63-1), an anti-angiogenic agent, was used as a positive control.

[0360] Table 12 Test Plan

[0361]

[0362] (2) Experimental results:

[0363] Table 13

[0364]

[0365] Note: When performing statistical analysis using the t-test method, significance compared with the BC group is indicated by #, P-value < 0.05 is indicated by #, and P.value < 0.001 is indicated by ###; significance compared with the NC group is indicated by *, P-value < 0.01 is indicated by **, and P.value < 0.001 is indicated by ***.

[0366] (3) Experimental conclusions (see Table 13) Figure 1 ):

[0367] 1) Compared with the BC group, the VEGF content in the NC group was significantly increased, indicating that the stimulation conditions in this experiment were effective. Compared with the NC group, the relative VEGF synthesis content in the PC (Suramin) group was significantly decreased, indicating that the positive control in this experiment was effective.

[0368] 2) Compared with the NC group, the effect of the sample group in inhibiting vasodilation was: Example 1 > Example 4.

[0369] Example 4: Facial melanin content test

[0370] (1) Experimental method:

[0371] Table 14 Test Plan

[0372]

[0373] (2) Experimental results:

[0374] Table 15

[0375]

[0376] Table 16

[0377]

[0378] Table 17

[0379]

[0380] Table 18

[0381]

[0382] according to Figure 6 It was found that after using the experimental product for 28 days, the dark circles under the eyes were significantly improved.

[0383] (3) Experimental conclusions (see Tables 15-18) Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 After 28 days of use, the L* value increased significantly, resulting in brighter eyes. The a* value decreased, reducing redness and inflammation around the eyes. The b* value increased, reducing blueness and decreasing melanin content, thus improving dark circles.

Claims

1. An oil and fat composition, characterized in that, The oil composition includes coffee oil, grapeseed oil, and coconut oil.

2. The oil composition according to claim 1, characterized in that, The mass ratio of the coffee oil, the grape seed oil, and the coconut oil is (0.5~2):(0.5~3.5):(3~10), for example (0.5~2):(1~3.5):(3~10), and even more preferably (0.5~1):(1~3.5):(6~10), and more preferably (0.5~1):(1~2):(6~8). For example, the mass ratio of coffee oil: grape seed oil: coconut oil is 0.5:3.5:6, 1:1:8, 2:3.5:3, 0.5:1:10, 2:2:6 or 2:0.5:

10.

3. The oil composition according to claim 1, characterized in that, The coffee oil was prepared using a supercritical fluid extraction method. For example, the coffee oil is prepared by the following method: It is prepared by freeze-drying, grinding, and supercritical fluid extraction of green coffee beans.

4. The oil composition according to claim 3, characterized in that, The method for preparing the coffee oil satisfies one or more of the following conditions: (1) The green coffee beans referred to are the seeds of Coffea Arabica L., a plant of the Rubiaceae family; (2) The freeze-drying process involves placing the food in a freeze dryer for freeze-drying. (3) The freeze-drying time is 12~26H, preferably 18~20H, for example 12H, 20H or 26H; (4) The pulverization is pulverization to pass through a 30-60 mesh sieve, preferably a 40-50 mesh sieve, for example a 30-50 mesh sieve; (5) The extraction is carried out in an extraction vessel; the amount of packing material to be extracted in the extraction vessel can be 50-70% of the vessel volume, more preferably 60-65%, for example 50%, 60% or 70%; (6) The extraction temperature is 33~58℃, preferably 45~50℃, for example 33℃, 50℃ or 58℃; (7) The extraction pressure is 15~35MPa, preferably 25~30MPa, for example 15MPa, 30MPa or 35MPa; (8) The supercritical fluid is supercritical CO2, and the flow rate of the supercritical CO2 can be 10~30 L / h, preferably 15~25 L / h, for example 10 L / h or 25 L / h; (9) The extraction time is 1-3 hours, for example, 1 hour, 2 hours, or 3 hours; and (10) The extraction is followed by a separation step, wherein the separation involves feeding a supercritical fluid carrying the product into a separator, releasing the supercritical fluid by depressurization, collecting the oil, and obtaining the coffee oil. The pressure of the separator can be 5~10MPa, preferably 5~8MPa, for example 5MPa, 7MPa or 8MPa; The oil can be filtered using a 0.5~1.0μm filter membrane, for example, a 0.8μm filter membrane.

5. The oil composition according to claim 1, characterized in that, The grape seed oil was obtained using a supercritical fluid extraction method. For example, the grape seed oil is obtained by the following method: It is prepared by supercritical fluid extraction of grape seed powder.

6. The oil composition according to claim 5, characterized in that, The method for preparing grape seed oil satisfies one or more of the following conditions: (11) The grape seeds mentioned refer to the seeds of grapes (Vitis vinifera L.), with a moisture content in the range of 2%-5%; (12) The grape seed powder is a powder that passes through a 20-50 mesh sieve, preferably a 30-40 mesh sieve, for example, a 20 mesh, 40 mesh or 50 mesh sieve; (13) The extraction is carried out in an extraction vessel; the amount of packing material to be extracted in the extraction vessel can be 50-70% of the vessel volume, more preferably 60-65%, for example 50%, 65% or 70%; (14) The extraction temperature is 30~60℃, preferably 45~50℃, for example 30℃, 50℃ or 60℃; (15) The extraction pressure can be 20~40MPa, preferably 25~30MPa, for example 20MPa, 30MPa or 40MPa; (16) The supercritical fluid may be supercritical CO2; the flow rate of the supercritical CO2 may be 10~30 L / h, preferably 15~25 L / h, for example 10 L / h, 25 L / h or 30 L / h; (17) The extraction time can be 1~3H, preferably 1.5~2H, for example 1H, 2H or 3H; and (18) The extraction is followed by a separation step, wherein the separation involves feeding a supercritical fluid carrying the product into a separator, releasing the supercritical fluid by depressurization, collecting the oil, and obtaining the coffee oil. The pressure of the separator can be 5~10MPa, preferably 5~8MPa, for example 5MPa, 7MPa or 8MPa; The oil can be filtered using a 0.5~1.0μm filter membrane, for example, a 0.8μm filter membrane.

7. The oil composition according to claim 1, characterized in that, The coconut oil was obtained using a supercritical fluid extraction method. For example, the coconut oil is obtained by the following method: The product is prepared by supercritical fluid extraction of coconut meat powder.

8. The oil composition according to claim 7, characterized in that, The method for preparing the coconut oil satisfies one or more of the following conditions: (19) The coconut flesh mentioned refers to the endosperm of the coconut (Cocos nucifera L.); (20) The coconut meat powder is a powder that passes through a 30-60 mesh sieve, preferably a 40-50 mesh sieve, for example, a 30 mesh, 40 mesh, 50 mesh or 60 mesh sieve; (21) The extraction is carried out in an extraction vessel; the amount of packing material to be extracted in the extraction vessel can be 50-70% of the vessel volume, more preferably 60-65%, for example 50%, 60%, 65% or 70%; (22) The extraction temperature is 30~50℃, preferably 40~45℃, for example 30℃, 40℃, 45℃ or 50℃; (23) The extraction pressure is 20~40MPa, preferably 25~30MPa, for example 20MPa, 25MPa, 30MPa or 40MPa; (24) wherein the supercritical fluid is supercritical CO2; the flow rate of the supercritical CO2 may be 10~30 L / h, more preferably 15~25 L / h, for example 10 L / h, 15 L / h, 25 L / h or 30 L / h; (25) The extraction time is 0.5~2H, preferably 1~1.5H, for example 0.5H, 1H, 1.5H or 2H; and (26) The extraction is followed by a separation step; the separation involves feeding a supercritical fluid carrying the product into a separator, releasing the supercritical fluid by depressurization, collecting the oil, and obtaining the coconut oil. The pressure of the separator can be 5~10MPa, preferably 5~8MPa, for example 5MPa, 6MPa, 7MPa or 8MPa; The oil can be filtered using a 0.5~1.0μm filter membrane, for example, a 0.8μm filter membrane.

9. The use of the oil composition as described in any one of claims 1-8 in a skin care product as an oil-soluble ingredient; The skincare products may be face creams, facial oils, lotions, or facial cleansers.

10. A skincare product, characterized in that, It comprises the oil and fat composition as described in any one of claims 1-8; Optionally, in the skin care product, the oil composition has a mass percentage of 1 to 10%, for example, 5%.

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