Cell culture tank for producing porcine circovirus vaccine

Abstract

This invention relates to the field of cell culture technology, specifically to a cell culture tank for producing porcine circovirus vaccine. The tank includes a main body with a feeding port at the top, a fixing box at the top, and a heat dissipation vent at the top of the fixing box. A stirring mechanism is located inside the main body. The first cone design achieves a shearing and flow-diverting effect, the arc surface guides the flow, reducing cell damage, and the spherical protrusions and through-holes further enhance the flow disturbance effect. The row of staggered spherical protrusions further improves the mixing effect. The two flow-dispersing blocks are arranged in opposite directions, achieving mixing in both forward and reverse rotation. The second cone design reduces resistance during reverse rotation without affecting mixing.

Description

Technical Field

[0001] This invention relates to the field of cell culture technology, specifically to a cell culture vessel for producing porcine circovirus vaccine. Background Technology

[0002] In the field of veterinary biological products, the production of porcine circovirus (PCV) vaccines relies on efficient cell culture processes to obtain sufficient viral antigens. Currently, PCV vaccines are mainly produced by culturing PK-15 cells, ST cells, or their derivatives in suspension or adherent form, followed by virus inoculation, amplification, harvesting, and purification. The cell culture process is typically carried out in a bioreactor (cell culture vessel), and its stirring, aeration, temperature control, and process control performance directly affect cell growth density, viability, and viral yield and quality.

[0003] A search revealed a patent with publication number CN222809474U, which discloses a cell bioreactor comprising a reaction vessel, a cover plate fixed to the top of the reaction vessel, a housing and a conduit fixed to the top of the cover plate, a notch and a support on one side of the housing, limit bolts on both sides of the housing, a motor fixed to the support, a connecting rod on one side of the motor, the end of the connecting rod away from the motor passing through the housing and connected to a handwheel, a vertical rod inside the reaction vessel, a first bevel gear and multiple horizontal rods fixed to the vertical rod, and the end of the connecting rod away from the handwheel and the electric... Each output shaft of the machine is fixed with a second bevel gear that matches the first bevel gear. By turning the handwheel, the handwheel drives the connecting rod to rotate, and the second bevel gear rotates accordingly. When the second bevel gear rotates, it drives the first bevel gear to rotate, causing the vertical and horizontal rods to rotate. Loosening the two limit bolts and pulling the support away from the connecting rod allows the support and motor to be removed for maintenance without affecting the mixing operation. However, in the above scenario, when the motor is driving, the handwheel rotates accordingly. When the equipment is mixing, if the operator is adding materials or observing the situation inside the tank, getting close to the handwheel can easily lead to danger.

[0004] Therefore, a cell culture vessel for producing porcine circovirus vaccine is proposed to solve the problems mentioned above. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a cell culture vessel for producing porcine circovirus vaccine, which can solve the aforementioned problems.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a culture tank body is provided, a feeding port is provided at the top of the culture tank body, a fixing box is provided at the top of the culture tank body, a heat dissipation vent is provided at the top of the fixing box, and a stirring mechanism is provided inside the culture tank body. The stirring mechanism includes a rotating shaft, a stirring rod, a turbulence block, a first cone, a second cone, a spherical protrusion, a protrusion, and a through hole. The rotating shaft is rotatably connected inside the culture tank body, the stirring rod is fixedly connected to the outside of the rotating shaft, the turbulence block is fixedly connected to the outside of the stirring rod, the first cone and the second cone are respectively located at both ends of the turbulence block, the spherical protrusion is fixedly connected to the outside of the first cone, the protrusion is located at the top and bottom of the turbulence block, and the through hole is opened on the outside of the protrusion.

[0007] Preferably, the port of the through hole is arc-shaped, and the ends of the first and second cones are both arc-shaped.

[0008] Preferably, the stirring rod is provided with two turbulence blocks, and the two turbulence blocks are oriented in opposite directions.

[0009] Preferably, the fixed box is provided with a transmission mechanism inside. The transmission mechanism includes a motor, a first bevel gear, a second bevel gear, a bracket, a third bevel gear, a polygonal column, a handle, and a polygonal socket. The motor is fixedly connected inside the fixed box, the first bevel gear is fixedly connected to the motor output end, the second bevel gear is fixedly connected to one end of the rotating shaft, the bracket is fixedly connected inside the fixed box, the third bevel gear is rotatably connected to the bracket, the polygonal column is fixedly connected to one end of the third bevel gear, the handle is disposed inside the fixed box, and the polygonal socket is opened at one end of the handle.

[0010] Preferably, the first bevel gear is meshed with the second bevel gear, and the second bevel gear is meshed with the third bevel gear.

[0011] Preferably, the inner wall of the polygonal socket fits against the outer wall of the polygonal column, and the polygonal socket is inserted into the outside of the polygonal column.

[0012] Preferably, a limiting plate is fixedly connected to the outside of the handle, a locking block is fixedly connected to the outside of the limiting plate, a through groove is opened on the outside of the limiting plate, a limiting groove is opened inside the fixing box, and a first positioning groove and a second positioning groove are opened inside the limiting groove.

[0013] Preferably, the first positioning groove and the second positioning groove are arranged in a ring shape, the outer wall of the limiting plate is in contact with the inner wall of the limiting groove, and the locking block is arranged in an arc shape.

[0014] Compared with the prior art, the present invention provides a cell culture vessel for the production of porcine circovirus vaccine, which has the following beneficial effects: 1. The first cone design achieves shearing and diversion effects, while the curved surface guides the flow and reduces damage to cells. The spherical protrusions and through holes further enhance the turbulence effect. The row of spherical protrusions with staggered arrangement further improves the mixing effect. The two turbulence blocks are arranged in opposite directions, achieving mixing in both forward and reverse rotation. The second cone design reduces resistance during reverse rotation without affecting mixing.

[0015] 2. By rotating the shaft with the handle, the cells and culture medium can be manually stirred, achieving a multi-functional stirring effect. This avoids the inability to mix cells during long-term maintenance, which can lead to cell deposition and ensure the stability of the culture process. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is a schematic cross-sectional view of the culture tank body of the present invention; Figure 3 This is a schematic diagram of the turbulence block structure of the present invention; Figure 4 This is a schematic diagram of the polygonal column structure of the present invention; Figure 5 This is a schematic diagram of the position structure of the limiting plate of the present invention.

[0017] In the diagram: 1. Culture tank body; 2. Feed port; 3. Fixing box; 4. Heat dissipation port; 5. Rotating shaft; 6. Stirring rod; 7. Baffle block; 8. First cone; 9. Second cone; 10. Spherical protrusion; 11. Protrusion; 12. Through hole; 13. Motor; 14. First bevel gear; 15. Second bevel gear; 16. Support; 17. Third bevel gear; 18. Polygonal column; 19. Handle; 20. Limiting plate; 21. Locking block; 22. Through groove; 23. Polygonal insertion hole; 24. Limiting groove; 25. First positioning groove; 26. Second positioning groove. Detailed Implementation

[0018] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Example:

[0019] Please see Figure 1 - Figure 5This embodiment of a cell culture tank for producing porcine circovirus vaccine includes a culture tank body 1, a feeding port 2 at the top of the culture tank body 1, a fixing box 3 at the top of the culture tank body 1, a heat dissipation port 4 at the top of the fixing box 3, and a stirring mechanism inside the culture tank body 1. The stirring mechanism includes a rotating shaft 5, a stirring rod 6, a turbulence block 7, a first cone 8, a second cone 9, a spherical protrusion 10, a protrusion 11, and a through hole 12. The rotating shaft 5 is rotatably connected inside the culture tank body 1, the stirring rod 6 is fixedly connected to the outside of the rotating shaft 5, the turbulence block 7 is fixedly connected to the outside of the stirring rod 6, the first cone 8 and the second cone 9 are respectively provided at both ends of the turbulence block 7, the spherical protrusion 10 is fixedly connected to the outside of the first cone 8, the protrusion 11 is provided at the top and bottom of the turbulence block 7, and the through hole 12 is opened on the outside of the protrusion 11. In use, the rotating shaft 5 rotates to stir the cells with the turbulence block 7. During stirring, the first cone 8 can achieve the effect of shearing and diverting, and the arc surface can guide the flow and reduce damage to the cells. The spherical protrusions 10 and the through holes 12 can further achieve the effect of turbulence. The spherical protrusions 10 are arranged in rows and staggered in each row to further improve the mixing effect. The two turbulence blocks 7 are arranged in opposite directions, which can achieve the mixing effect in both forward and reverse rotation. The second cone 9 can reduce resistance in reverse rotation without affecting the mixing.

[0020] Please see Figure 1 - Figure 5 The port of the through hole 12 is arc-shaped, and the ends of the first cone 8 and the second cone 9 are both arc-shaped. Two turbulence blocks 7 are provided on the stirring rod 6, and the two turbulence blocks 7 are in opposite directions.

[0021] Please see Figure 1 - Figure 5The fixed box 3 is equipped with a transmission mechanism, which includes a motor 13, a first bevel gear 14, a second bevel gear 15, a bracket 16, a third bevel gear 17, a polygonal column 18, a handle 19, and a polygonal socket 23. The motor 13 is fixedly connected inside the fixed box 3. The first bevel gear 14 is fixedly connected to the output end of the motor 13. The second bevel gear 15 is fixedly connected to one end of the rotating shaft 5. The bracket 16 is fixedly connected inside the fixed box 3. The third bevel gear 17 is rotatably connected to the bracket 16. The polygonal column 18 is fixedly connected to one end of the third bevel gear 17. The handle 19 is located inside the fixed box 3. The polygonal socket 23 is opened at one end of the handle 19. A limit plate 20 is fixedly connected to the outside of the handle 19. A locking block 21 is fixedly connected to the outside of the limit plate 20. A through groove 22 is opened on the outside of the limit plate 20. A limit groove 24 is opened inside the fixed box 3. A first positioning groove 25 and a second positioning groove 26 are opened inside the limit groove 24.

[0022] The motor 13 can drive the rotating shaft 5 to rotate. If the motor 13 is damaged and needs repair, push the handle 19 to make the limiting plate 20 slide inside the limiting groove 24. While sliding, the locking block 21 disengages from the first positioning groove 25 and locks into the second positioning groove 26. At this time, the polygonal insertion hole 23 is inserted into the outside of the polygonal column 18. By rotating the handle 19, the rotating shaft 5 can be driven to rotate, and the cells and culture medium can be manually stirred to achieve a multi-functional stirring effect. This avoids the situation where cells cannot be mixed due to long-term maintenance, which may lead to cell deposition and ensure the stability of the culture process. The locking block 21 is designed to work in conjunction with the first positioning groove 25 and the second positioning groove 26 to achieve a positioning effect whether the handle 19 is used or not, thus preventing the handle 19 from being accidentally inserted into the polygonal column 18 when not in use, which would cause the handle 19 to rotate automatically and affect operational safety.

[0023] Please see Figure 1 - Figure 5 The first bevel gear 14 meshes with the second bevel gear 15, and the second bevel gear 15 meshes with the third bevel gear 17. The inner wall of the polygonal insertion hole 23 fits against the outer wall of the polygonal column 18, and the polygonal insertion hole 23 is inserted into the outside of the polygonal column 18. The first positioning groove 25 and the second positioning groove 26 are annularly arranged. The outer wall of the limiting plate 20 fits against the inner wall of the limiting groove 24. The locking block 21 is arc-shaped. The working principle of the above embodiments is as follows: In use, the rotating shaft 5 rotates to stir the cells with the turbulence block 7. During stirring, the first cone 8 can achieve the effect of shearing and diverting, and the arc surface can guide the flow and reduce damage to the cells. The spherical protrusions 10 and the through holes 12 can further achieve the effect of turbulence. The spherical protrusions 10 are arranged in rows and staggered in each row to further improve the mixing effect. The two spoilers 7 are positioned in opposite directions, which can achieve a mixing effect in both forward and reverse rotation. Furthermore, the second cone 9 can reduce resistance in reverse rotation without affecting the mixing. The motor 13 can drive the rotating shaft 5 to rotate. If the motor 13 is damaged and needs repair, push the handle 19 to make the limiting plate 20 slide inside the limiting groove 24. While sliding, the locking block 21 disengages from the first positioning groove 25 and locks into the second positioning groove 26. At this time, the polygonal insertion hole 23 is inserted into the outside of the polygonal column 18. By rotating the handle 19, the rotating shaft 5 can be driven to rotate, and the cells and culture medium can be manually stirred to achieve a multi-functional stirring effect. This avoids the situation where cells cannot be mixed due to long-term maintenance, which may lead to cell deposition and ensure the stability of the culture process. The locking block 21 is designed to work in conjunction with the first positioning groove 25 and the second positioning groove 26 to achieve a positioning effect whether the handle 19 is used or not, thus preventing the handle 19 from being accidentally inserted into the polygonal column 18 when not in use, which would cause the handle 19 to rotate automatically and affect operational safety.

[0024] The installation, connection, or setting methods disclosed in this embodiment are all common mechanical connection methods. As long as they can achieve their beneficial effects, they can be implemented. Therefore, this embodiment will not elaborate on their specific structural composition and working principle.

[0025] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A cell culture vessel for producing porcine circovirus vaccine, characterized in that: The culture tank includes a culture tank body (1), a feeding port (2) is provided on the top of the culture tank body (1), a fixing box (3) is provided on the top of the culture tank body (1), a heat dissipation port (4) is provided on the top of the fixing box (3), and a stirring mechanism is provided inside the culture tank body (1). The stirring mechanism includes a rotating shaft (5), a stirring rod (6), a turbulence block (7), a first cone (8), a second cone (9), a spherical protrusion (10), a protrusion (11), and a through hole (12). The rotating shaft (5) is rotatably connected inside the culture tank body (1), the stirring rod (6) is fixedly connected to the outside of the rotating shaft (5), the turbulence block (7) is fixedly connected to the outside of the stirring rod (6), the first cone (8) and the second cone (9) are respectively set at both ends of the turbulence block (7), the spherical protrusion (10) is fixedly connected to the outside of the first cone (8), the protrusion (11) is set at the top and bottom of the turbulence block (7), and the through hole (12) is opened outside the protrusion (11).

2. The cell culture vessel for producing porcine circovirus vaccine according to claim 1, characterized in that: The port of the through hole (12) is arc-shaped, and the ends of the first cone (8) and the second cone (9) are both arc-shaped.

3. The cell culture vessel for producing porcine circovirus vaccine according to claim 1, characterized in that: Two turbulence blocks (7) are provided on the stirring rod (6), and the two turbulence blocks (7) are in opposite directions.

4. The cell culture vessel for producing porcine circovirus vaccine according to claim 1, characterized in that: The fixed box (3) is equipped with a transmission mechanism, which includes a motor (13), a first bevel gear (14), a second bevel gear (15), a bracket (16), a third bevel gear (17), a polygonal column (18), a handle (19), and a polygonal socket (23). The motor (13) is fixedly connected inside the fixed box (3), the first bevel gear (14) is fixedly connected to the output end of the motor (13), the second bevel gear (15) is fixedly connected to one end of the rotating shaft (5), the bracket (16) is fixedly connected inside the fixed box (3), the third bevel gear (17) is rotatably connected to the bracket (16), the polygonal column (18) is fixedly connected to one end of the third bevel gear (17), the handle (19) is located inside the fixed box (3), and the polygonal socket (23) is located at one end of the handle (19).

5. The cell culture vessel for producing porcine circovirus vaccine according to claim 4, characterized in that: The first bevel gear (14) meshes with the second bevel gear (15), and the second bevel gear (15) meshes with the third bevel gear (17).

6. The cell culture vessel for producing porcine circovirus vaccine according to claim 4, characterized in that: The inner wall of the polygonal socket (23) fits against the outer wall of the polygonal column (18), and the polygonal socket (23) is inserted into the outside of the polygonal column (18).

7. The cell culture vessel for producing porcine circovirus vaccine according to claim 4, characterized in that: The handle (19) is fixedly connected to a limiting plate (20), and a locking block (21) is fixedly connected to the outside of the limiting plate (20). A through groove (22) is opened on the outside of the limiting plate (20), and a limiting groove (24) is opened inside the fixing box (3). A first positioning groove (25) and a second positioning groove (26) are opened inside the limiting groove (24).

8. The cell culture vessel for producing porcine circovirus vaccine according to claim 7, characterized in that: The first positioning groove (25) and the second positioning groove (26) are arranged in a ring shape. The outer wall of the limiting plate (20) is in contact with the inner wall of the limiting groove (24). The card block (21) is arranged in an arc shape.

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