Application of stevia low-phosphorus response gene spx family in improving stevia glycoside content

By overexpressing SrSPX2 and SrSPX-RING4, members of the SPX family of stevia low-phosphorus response genes, glycoside synthesis was regulated, solving the problem of limited yield and quality of stevia under low phosphorus stress. This resulted in the improvement of stevia glycoside content and dynamic regulation of phosphorus transport, promoting the tolerance of stevia to low phosphorus stress and the sustainable development of the industry.

CN122168619APending Publication Date: 2026-06-09CHENGDU NORMAL UNIV
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Patent Information

Application Number
CN202610138204.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-01-31
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Stevia glycoside synthesis is significantly affected by low phosphorus stress. Existing studies have not clarified the role of key gene families in phosphorus signaling pathways in glycoside synthesis, which leads to limited stevia yield and quality. In addition, traditional application of phosphate fertilizers is inefficient and easily leads to resource waste and environmental pollution.

Method used

By overexpressing SrSPX2 and SrSPX-RING4, members of the SPX family of stevia low-phosphorus response genes, glycoside synthesis was regulated, increasing the content of steviol glycoside, rebaudioside C, and rebaudioside D, while decreasing the content of rebaudioside A, steviol disaccharides, and total glycosides, thus enhancing the low-phosphorus stress tolerance of stevia.

Benefits of technology

It significantly improves the aboveground growth of stevia, increases glycoside content, improves stevia yield and quality, dynamically regulates phosphorus signals, optimizes phosphorus transport, reduces resource waste, and promotes the green and sustainable development of the industry.

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Abstract

The application belongs to the field of agricultural biotechnology, and particularly relates to application of a low-phosphorus response gene SPX family of Stevia rebaudiana in improving glycoside content of Stevia rebaudiana. The application provides application of a low-phosphorus response gene SPX family of Stevia rebaudiana in improving glycoside content of Stevia rebaudiana, wherein the low-phosphorus response gene SPX family of Stevia rebaudiana comprises SrSPX2 a gene and SrSPX-RING4 a gene, a nucleotide sequence of the SrSPX2 gene is shown as SEQ ID NO: 1, and a nucleotide sequence of the SrSPX-RING4 gene is shown as SEQ ID NO: 2. The application determines the influence of low phosphorus on yield and quality of Stevia rebaudiana, and explores potential application value of a phosphorus regulation gene in improving quality of Stevia rebaudiana.
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