A wheat breeding material single plant precision sampling and marking integrated device

Abstract

This invention relates to the field of agricultural breeding technology and discloses an integrated device for precise sampling and labeling of single wheat breeding materials. The device includes a feeding drive mechanism, a photoacoustic coaxial cutting and in-situ labeling module, a carrier-type collection system, a microfluidic system, and a central control system. The device utilizes high-frequency mechanical vibration to cut plant tissue, combined with cavitation effects to prevent sample contamination from the cutting edge. The cut sample falls into the carrier for sealed encapsulation and digital identification. The module pumps liquid photosensitive resin to the plant wound, and through ultrasonic-induced shear thinning and acoustic flow effects, promotes deep resin penetration. A coaxial ultraviolet beam is then output for in-situ curing, forming a peel-resistant, mechanically interlocked label patch. The main control system extracts the sample identifier, timestamp, and spectral characteristics of quantum dots within the resin, generating an encrypted hash value for persistent storage. This invention achieves high-purity, precise sampling, robust in-situ labeling, and tamper-proof multidimensional data traceability.

Description

Technical Field

[0001] The present invention relates to the technical field of agricultural breeding, and specifically provides an integrated device for precise sampling and marking of individual wheat breeding materials. Background Art

[0002] In the molecular breeding process of crops such as wheat, the rapid sampling of individual plant leaves and the precise marking of maternal plants are the basic links for establishing the corresponding relationship between genotypes and phenotypes. In the current mainstream mechanical sampling equipment, when continuously performing cutting actions, the juice and nucleic acid macromolecules released by the rupture of plant tissues are extremely likely to adhere to the surface of the cutting tool. Due to the lack of effective in-situ blocking and physical cleaning mechanisms, the organic matter residues on the surface of the cutting tool will cause cross-contamination between different plant samples obtained subsequently, directly affecting the accuracy of downstream gene sequencing and genotyping data. At the same time, the existing field plant marking methods mostly use external physical tags or surface-applied chemical dyes. These markers only stay on the outer epidermis of the plant. Under the action of complex natural environmental stresses in the field and the growth of the plant's own tissues, the markers are prone to mechanical peeling or degradation and failure, and it is difficult to form a long-term reliable anchoring.

[0003] The existing sampling equipment usually separates the physical cutting and collection actions from the data tracking and management link in the system architecture. The in-vitro plant tissue samples, the maternal plants staying in the field, and the sample numbers in the experimental information system often rely on independent data collection terminals for post-event matching. This separated operation mode lacks a mechanism for automatically binding the identifier of the in-vitro sample, the in-situ marking characteristics of the maternal plant, and the spatio-temporal state information at the hardware level at the moment of cutting the plant tissue. During the entire sample transfer and breeding growth cycle, the loose data association logic is prone to cause sample identity confusion and there is a risk of data traceability chain breakage, which is difficult to meet the hardware-level management requirements of high-throughput breeding processes for strong data consistency and anti-tampering attributes.

[0004] In view of this, the purpose of the present invention is to provide an integrated device for precise sampling and marking of individual wheat breeding materials to solve the deficiencies existing in the prior art. Summary of the Invention

[0005] Aiming at the deficiencies of the prior art, the present invention provides an integrated device for precise sampling and marking of individual wheat breeding materials, which solves the problems of cross-sample cross-contamination caused by organic matter residues on the surface of the cutting tool during continuous sampling in existing breeding, easy shedding and failure of in-situ plant marking due to environmental stress, and separation and breakage of the data traceability chain between in-vitro samples and field maternal plants.

[0006] To achieve the above objectives, this invention provides the following technical solution: an integrated device for precise sampling and labeling of single wheat breeding materials, comprising a feeding drive mechanism, a photoacoustic coaxial cutting and in-situ labeling module, a carrier-type encapsulation and collection system, a microfluidic liquid supply and pneumatic auxiliary system, and a central timing master control and data interconnection system. The feeding drive mechanism responds to control commands output by the central timing master control and data interconnection system, driving the photoacoustic coaxial cutting and in-situ labeling module to feed towards the target plant tissue. During the feeding process, the photoacoustic coaxial cutting and in-situ labeling module outputs high-frequency mechanical vibration to cut and separate the target plant tissue, obtaining the cut plant tissue. The carrier-type encapsulation and collection system receives the cut plant tissue and performs sealed encapsulation and string sequence identification reading. The microfluidic liquid supply and pneumatic auxiliary system quantitatively pumps liquid photosensitive resin into the photoacoustic coaxial cutting and in-situ labeling module. The photoacoustic coaxial punching and in-situ marking module injects liquid photosensitive resin into the plant cutting wound and coaxially outputs ultraviolet light beams to irradiate the liquid photosensitive resin, causing it to undergo a photopolymerization and cross-linking reaction, forming a solid resin marking patch that establishes a logical mapping relationship with the string sequence identifier.

[0007] The photoacoustic coaxial punching and in-situ marking module includes a sandwich-type piezoelectric transducer, a hollow preload bolt, and an ultraviolet light-emitting diode array substrate assembly. A cylindrical through-hole is formed inside the sandwich-type piezoelectric transducer, and the hollow preload bolt is fitted into the cylindrical through-hole, forming a coaxial hollow optical path channel within it. The ultraviolet light-emitting diode array substrate assembly emits an ultraviolet beam, which enters and penetrates the entire coaxial hollow optical path channel axially. The photoacoustic coaxial punching and in-situ marking module is also equipped with a through-type ultraviolet light-conducting ultrasonic amplitude transformer, utilizing the substrate material properties of this through-type ultraviolet light-conducting ultrasonic amplitude transformer to synchronously transmit high-frequency mechanical vibration and ultraviolet beam along the same physical axis. The through-type ultraviolet light guide ultrasonic amplitude transformer has a microfluidic internal flow channel inside. The bottom opening of the central axial liquid outlet section of the microfluidic internal flow channel and the emission area of ​​the ultraviolet beam are both located inside the annular punching blade at the bottom of the through-type ultraviolet light guide ultrasonic amplitude transformer, realizing the spatial combination of physical mechanical vibration energy transmission and optical ultraviolet radiation energy transmission on the same physical axis.

[0008] The microfluidic liquid supply and pneumatically assisted system includes a light-shielded liquid reservoir and a piezoelectric micropump. The microfluidic internal flow channel includes a side-walled inclined inlet section, the axial spatial coordinates of which are set on the nodal cross-section of the standing wave field distribution propagating along the through-type ultraviolet light guide ultrasonic amplitude transformer. The piezoelectric micropump receives voltage pulse signals and pumps the liquid photosensitive resin from the light-shielded reservoir into the microfluidic internal flow channel through the inlet of the side-walled inclined inlet section. This nodal cross-section design blocks the mechanical transmission leakage of high-frequency vibration energy, preventing alternating stress from damaging the seal at pipe connections.

[0009] In the fluid infiltration sequence, the alternating mechanical stress output by the through-type ultraviolet light-conducting ultrasonic amplitude transformer acts on the liquid photosensitive resin, triggering the non-Newtonian fluid shear-thinning physical property, thereby reducing the apparent dynamic viscosity. Simultaneously, acoustic radiation pressure is generated within the fluid medium to induce acoustic flow, driving the low-viscosity liquid photosensitive resin to overcome the flow resistance of capillary pores within the plant, and to conduct directional fluid infiltration into the exposed vascular bundles and intercellular spaces of the plant's cut wound. Subsequently, photopolymerization and cross-linking reactions occur through ultraviolet light irradiation, generating a solid resin-labeled patch, which forms a mechanically interlocked structure within the plant's microstructure.

[0010] During the cutting process, a through-type ultraviolet light-guided ultrasonic amplitude transformer drives the annular punching blade to maintain a fixed displacement amplitude of high-frequency mechanical vibration. This induces an alternating acoustic pressure field and generates cavitation bubbles in the contact area between the annular punching blade end face and the released plant sap medium. The microjets generated by the pressure collapse of these cavitation bubbles are used to block the intermolecular adhesion of plant organic macromolecules to the boundary layer of the annular punching blade's metal surface, thus eliminating material residue correlations between cross-sample operations.

[0011] The carrier tape-type encapsulation and collection system includes a base tape, a heat-sealing film, a heat-sealing assembly, and a machine vision barcode reader. The base tape has evenly spaced micropores, and its second surface is printed with a machine-readable QR code data array. The heat-sealing assembly thermally cross-links the heat-sealing film, which covers the area above the micropores containing the cut plant tissue, with the first surface of the base tape. The machine vision barcode reader acquires an image of the corresponding area of ​​the machine-readable QR code data array and decodes it into a string sequence identifier, completing the data acquisition of the physical sample.

[0012] The central timing master control and data interconnection system includes a microcontroller chip. For a single sampling and marking operation cycle, the microcontroller chip sequentially outputs a positive drive level signal, an ultrasonic enable signal, a control command with a specific number of pulses, an image acquisition trigger level, a preset number of electrical pulse signals, and an on-level signal for a specific duration, according to the timing sequence. The microcontroller chip integrates a hardware timer that calculates the target exposure time value based on the preset resin critical energy density and the pre-measured output light intensity. After the continuous output time of the on-level signal reaches the target exposure time value, the on-level signal is toggled to the off level.

[0013] The central timing control and data interconnection system extracts the string sequence identifier generated by the machine vision barcode reader, the absolute timestamp latched when a single cutting action is completed, and the characteristic spectral data of the inorganic fluorescent quantum dots pre-placed in liquid photosensitive resin for this batch, combining them to construct a comprehensive traceability data tuple. A one-way encrypted hash algorithm function is then called to perform operations on the comprehensive traceability data tuple to generate a verification hash value, completing the persistent storage of the logical mapping relationship.

[0014] This invention provides an integrated device for precise sampling and labeling of single wheat breeding materials. It has the following beneficial effects:

[0015] 1. This invention utilizes the high-frequency alternating mechanical stress output by a through-type ultraviolet light guide ultrasonic amplitude transformer to trigger the shear thinning physical property of liquid photosensitive resin, and uses the directional acoustic flow induced by acoustic radiation pressure to drive the low-viscosity resin to penetrate into the vascular bundles and intercellular spaces of the plant. Subsequently, the in-situ photopolymerization cross-linking reaction is completed through a coaxially transmitted ultraviolet light beam, so that the solid resin label patch and the plant micro-tissue form a stable mechanical interlocking structure, preventing the label from falling off during subsequent plant growth and field management.

[0016] 2. This invention drives a ring-shaped cutting blade to maintain a fixed displacement amplitude of high-frequency mechanical vibration in the released plant sap medium, inducing an alternating sound pressure field and generating a cavitation effect. The microjets generated by the collapse of cavitation bubbles under pressure can effectively block the intermolecular adhesion of plant organic macromolecules at the boundary layer of the metal blade, maintain the physical cleanliness of the cutting end face, and thus ensure that the obtained cut plant tissue has high purity, meeting the requirements of molecular breeding experiments.

[0017] 3. This invention, through a central timing master control and data interconnection system, combines the string sequence identifiers decoded by the machine vision barcode reader, the absolute timestamp of a single cutting action, and the characteristic spectral data of inorganic fluorescent quantum dots solidified in situ on the plant to construct a comprehensive traceability data tuple. It further calls a one-way encrypted hash algorithm function to perform operations on the data tuple to generate a verification hash value and persistently store it, thereby realizing a definite logical binding between the encapsulated sample, the field mother plant, and the database code, ensuring the consistency and traceability of the breeding data chain. Attached Figure Description

[0018] Figure 1 This is a diagram of the device architecture of the present invention;

[0019] Figure 2 This is a schematic diagram of the microcontroller-based timing closed-loop logic architecture of the present invention;

[0020] Figure 3 This is a schematic diagram comparing the concentration of residual DNA on the blade surface under different punching modes of the present invention;

[0021] Figure 4 This is a schematic diagram comparing the peel strength of different marking methods of the present invention in a field environment. Detailed Implementation

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] See attached document Figure 1 This invention provides an integrated device for precise sampling and marking of single wheat breeding materials. The device includes: a main frame structure, a feeding drive mechanism, a carrier tape packaging and collection system, and a photoacoustic coaxial punching and in-situ marking module. The photoacoustic coaxial punching and in-situ marking module, the feeding drive mechanism, and the carrier tape packaging and collection system are all mechanically fixed inside the main frame structure.

[0024] The main frame structure has an external outline resembling a handheld gun, and its interior is equipped with a metal partition plate that divides the internal space of the device into a drive chamber and a collection chamber. The bottom plane of the main frame structure has a robotic arm connection flange, which is fixedly connected to the external mechanical actuator by fastening bolts.

[0025] The feed drive mechanism is installed inside the drive cavity and includes a linear guide rail, a slider assembly, and a pneumatic miniature cylinder. The linear guide rail is fixed to the metal partition plate with screws. The slider assembly is slidably mounted on the surface of the linear guide rail, and the telescopic piston rod of the pneumatic miniature cylinder is fixedly connected to the slider assembly. The photoacoustic coaxial punching and in-situ marking module is fixed to the slider assembly via a flange. The pneumatic miniature cylinder drives the photoacoustic coaxial punching and in-situ marking module to perform linear reciprocating feed motion along the Z-axis.

[0026] During the process of the photoacoustic coaxial punching and in-situ marking module making contact with and pressing the target plant leaf, the actual output and static contact pressure applied to the plant leaf surface satisfies the following physical relationship:

[0027] ;

[0028] In the formula, Defined as the static contact pressure exerted on plant leaves by the photoacoustic coaxial punching and in-situ marking module; Defined as the internal control air pressure at the air inlet end of a pneumatic miniature cylinder; Defined as atmospheric pressure in the external environment; Defined as the effective cross-sectional area of ​​the piston inside a pneumatic miniature cylinder under force. Defined as the total mass of the photoacoustic coaxial punching and in-situ marking module together with the slider assembly; Defined as a constant of gravitational acceleration; Defined as the mechanical sliding friction force generated when the slider assembly moves along the linear guide rail.

[0029] A carrier tape packaging and collection system is installed within the collection cavity. The system includes a stepper motor, an unwinding shaft, a take-up shaft, a base tape, and a heat-sealing film. The base tape is wound around the outside of the unwinding shaft, and has evenly spaced micro-perforations extending through its thickness along its longitudinal direction. The first surface of the base tape is covered with the heat-sealing film. The second surface of the base tape has a machine-readable QR code data array printed at adjacent positions corresponding to each micro-perforation. The ends of the base tape and the heat-sealing film are jointly and securely wound onto the take-up shaft. The output shaft of the stepper motor is mechanically connected to the take-up shaft, driving its rotation to achieve synchronous stepping transmission of the base tape and the heat-sealing film.

[0030] The collection chamber contains a sampling support base. The horizontal transmission path of the bottom strip runs parallel to and fits through the upper surface of the sampling support base. A vertically penetrating sample drop channel is located directly above the sampling support base. When the pneumatic micro-cylinder drives the photoacoustic coaxial punching and in-situ marking module to descend to the lower displacement stop point, the central longitudinal axis of the photoacoustic coaxial punching and in-situ marking module is completely aligned with the geometric central axis of the currently stationary micro-orifice on the bottom strip.

[0031] The carrier tape packaging and collection system also includes a heat-sealing assembly and a machine vision barcode reader. The heat-sealing assembly is located on the downstream bottom tape transport path on one side of the sampling carrier base. The heat-sealing assembly includes an electrically heated roller and a pre-tensioning spring. The outer surface of the electrically heated roller directly contacts the outer surface of the heat-melt sealing film, sealing the edge of the heat-melt sealing film to the first surface of the bottom tape through heat conduction. The machine vision barcode reader is fixedly mounted at the opening directly below the sampling carrier base. The optical lens of the machine vision barcode reader faces the second surface of the bottom tape, and is used to acquire images of the machine-readable QR code data array on the second surface of the bottom tape during the stepper motor pause interval, and send the data to an external control unit through a communication interface.

[0032] The photoacoustic coaxial punching and in-situ marking module includes a sandwich-type piezoelectric transducer. The sandwich-type piezoelectric transducer consists of a rear cover plate, a front cover plate, a piezoelectric ceramic stack located between the rear and front cover plates, and multiple annular metal electrode plates sandwiched within the piezoelectric ceramic stack. The piezoelectric ceramic stack comprises multiple piezoelectric ceramic rings alternately stacked along the axial polarization direction. The front cover plate is made of hard aluminum alloy, and the rear cover plate is made of structural steel. The annular metal electrode plates have positive and negative terminals for connecting to high-frequency AC signals output from an external ultrasonic generator.

[0033] The rear cover plate, the piezoelectric ceramic stack, and the front cover plate all have central circular holes of equal diameter at their geometric center axes. The sandwich piezoelectric transducer also includes a hollow preload bolt. This bolt passes through the central circular holes in the rear cover plate and the piezoelectric ceramic stack, and engages with an internally threaded section inside the front cover plate. By applying a set tightening torque, the hollow preload bolt applies axial static compressive stress to the piezoelectric ceramic stack to counteract the tensile stress generated by the piezoelectric ceramics under high-frequency vibration. The hollow preload bolt has a cylindrical through-hole running axially through its interior. This cylindrical through-hole forms a coaxial hollow optical path channel allowing optical energy to penetrate the entire interior of the sandwich piezoelectric transducer.

[0034] A sealing sleeve is fitted to the outer end of the rear cover away from the piezoelectric ceramic stack. Inside the sealing sleeve is a sealed light source chamber. An ultraviolet light-emitting diode (UVLED) array substrate assembly is fixedly mounted on the bottom surface of the sealed light source chamber, and metal heat sink fins are fitted to the back of the array substrate assembly. The geometric position of the central light-emitting point of the UVLED array substrate assembly is on the same spatial straight line as the central geometric axis of the sandwich piezoelectric transducer, ensuring that the light source output axis coincides with the mechanical vibration axis.

[0035] A collimating lens assembly is fixed inside the enclosed cavity of the light source between the emitting surface of the ultraviolet light-emitting diode array substrate assembly and the light-inlet port of the hollow preload bolt. The collimating lens assembly includes an aspherical condensing lens and a collimating plano-convex lens, which are coaxially fixed along the optical path transmission direction. The initially divergent ultraviolet beam emitted by the ultraviolet light-emitting diode array substrate assembly passes sequentially through the aspherical condensing lens and the collimating plano-convex lens, and is converted into a parallel ultraviolet beam after optical refraction. The maximum outer diameter of the parallel ultraviolet beam is smaller than the inner diameter of the cylindrical through-hole inside the hollow preload bolt. The converted parallel ultraviolet beam directly enters and penetrates the coaxial hollow optical path channel inside the hollow preload bolt along the axial direction, and finally exits axially outward from the center hole on the bottom end face of the front cover plate. Through this structure, the transmission of physical mechanical vibration energy and the transmission of optical ultraviolet radiation energy are physically isolated and spatially combined along the same physical axis.

[0036] The photoacoustic coaxial punching and in-situ marking module also includes a through-type ultraviolet light-guided ultrasonic amplitude transformer. The top input surface of the through-type ultraviolet light-guided ultrasonic amplitude transformer is coaxially mechanically connected to the bottom surface of the front cover plate of the sandwich piezoelectric transducer via a bolt fastening structure. The substrate material of the through-type ultraviolet light-guided ultrasonic amplitude transformer is made of fused silica glass or single-crystal sapphire. This substrate material possesses the properties of an elastic solid medium, allowing high-frequency elastic longitudinal waves to propagate within it; simultaneously, this substrate material possesses optical transmission properties, allowing ultraviolet beams with wavelengths in the 365nm to 395nm range to undergo refraction and total internal reflection transmission within it.

[0037] The external profile of the through-type ultraviolet light-guided ultrasonic amplitude transformer is a stepped, axisymmetric rotating structure. Along the vertically downward axial direction, the cross-sectional area of ​​the through-type ultraviolet light-guided ultrasonic amplitude transformer exhibits a geometrically decreasing step-like reduction characteristic. The longitudinal mechanical vibration wave generated by the sandwich-type piezoelectric transducer is transmitted to the through-type ultraviolet light-guided ultrasonic amplitude transformer via the top input surface. When the mechanical vibration wave propagates along the region with the smaller cross-sectional area, due to the physical constraint of conservation of mechanical kinetic energy, the longitudinal mechanical displacement amplitude at the lower end face of the through-type ultraviolet light-guided ultrasonic amplitude transformer is greater than that at the top input surface.

[0038] For the optical transmission path, the through-type ultraviolet light guiding ultrasonic amplitude transformer body constitutes a cylindrical optical waveguide structure. A parallel ultraviolet beam emitted from the cylindrical through-hole inside the sandwich piezoelectric transducer passes perpendicularly through the top input surface of the through-type ultraviolet light guiding ultrasonic amplitude transformer and enters its interior. The ultraviolet beam entering the interior undergoes total internal reflection at the physical boundary of the sidewall of the through-type ultraviolet light guiding ultrasonic amplitude transformer, is confined within the material, and propagates axially downwards, ultimately exiting from the bottom output end face of the through-type ultraviolet light guiding ultrasonic amplitude transformer.

[0039] The through-type ultraviolet light-conducting ultrasonic amplitude transformer has a microfluidic internal flow channel. This microfluidic internal flow channel includes a sidewall-angled inlet section and a central axial outlet section. The inlet of the sidewall-angled inlet section is located near the upper end of the outer cylindrical surface of the through-type ultraviolet light-conducting ultrasonic amplitude transformer. The central axial outlet section extends along the geometric central axis, with its bottom opening located at the center of the lowest output end face of the through-type ultraviolet light-conducting ultrasonic amplitude transformer. The sidewall-angled inlet section and the central axial outlet section are internally physically connected. This spatial arrangement avoids physical interference between the internal fluid channel and the ultraviolet beam incident area on the top surface.

[0040] The bottom edge of the output face of the through-type ultraviolet light-conducting ultrasonic amplitude transformer is machined with a protruding annular cutting edge. The end face contour of the annular cutting edge is a closed ring. The bottom opening of the central axial liquid outlet section and the ultraviolet light emission area are both located within the inner circular area enclosed by the annular cutting edge. Under the combined action of longitudinal static pressure applied by the pneumatic micro-cylinder and longitudinal mechanical vibration of ultrasonic frequency, the annular cutting edge cuts into and severs the fibrous structure of the target plant tissue, so that the severed plant tissue presents a circular sheet-like structure with the same inner diameter as the annular cutting edge.

[0041] The microfluidic liquid supply and pneumatic auxiliary system includes a light-shielded liquid reservoir, a piezoelectric micropump, a miniature check valve, and a liquid supply pipeline network. The light-shielded liquid reservoir is used to seal and contain the liquid photosensitive resin mixture. The shell material of the light-shielded liquid reservoir is opaque, blocking external ambient light from entering its internal chamber and preventing the liquid photosensitive resin from undergoing a spontaneous photopolymerization crosslinking reaction before receiving a control command.

[0042] The fluid outlet of the light-proof liquid reservoir is connected to the fluid inlet of the piezoelectric micropump via a first sealed conduit. The hardware structure of the piezoelectric micropump includes a piezoelectric ceramic actuator and a miniature pump chamber. When a voltage pulse signal of a specific frequency and amplitude is received from an external control unit, the piezoelectric ceramic actuator undergoes piezoelectric deformation, changing the internal geometric volume of the miniature pump chamber, thereby pumping a specific volume of liquid photosensitive resin from the fluid inlet to the fluid outlet.

[0043] The fluid outlet of the piezoelectric micro-pump is connected to the input end of a miniature check valve via a second sealed conduit. The output end of the miniature check valve is connected to a flexible delivery tube. A mechanical fastening connector is fixedly fitted to the end of the flexible delivery tube. This mechanical fastening connector is mechanically screwed onto the outer side of the through-type ultraviolet light-conducting ultrasonic amplitude transformer to form a fluid sealing boundary. This connection position is aligned with the inlet of the inclined liquid inlet section on the side wall, creating a continuous and unobstructed physical flow channel between the external flexible delivery tube and the internal inclined liquid inlet section on the side wall and the central axial liquid outlet section.

[0044] The physical coordinates of the inclined liquid inlet section on the side wall, along the axial direction of the through-type ultraviolet light-conducting ultrasonic amplitude transformer, are located on the nodal section of the standing wave field propagating along the amplitude transformer. Based on acoustic theory calculations and the standing wave waveform distribution, the axial high-frequency mechanical vibration displacement corresponding to this nodal section approaches zero. Setting the physical connection point of the external pipeline at the nodal section eliminates the destructive effect of alternating stress caused by the high-frequency vibration of the amplitude transformer on the sealing of the mechanical fastening joint, and also blocks the mechanical transmission leakage of high-frequency vibration energy towards the flexible delivery pipe and the miniature one-way valve.

[0045] The miniature one-way valve contains an elastic sealing diaphragm. The one-way opening direction of this elastic sealing diaphragm is consistent with the direction in which the liquid photosensitive resin is injected into the amplitude transformer. When the pneumatic miniature cylinder drives the photoacoustic coaxial punching and in-situ marking module to move linearly downwards or upwards along the Z-axis, the flexible delivery tube compensates for the spatial linear displacement difference by relying on the physical deformation capability of its material. During the period when the through-type ultraviolet light guide ultrasonic amplitude transformer is in the punching and cutting stage and generates hydrostatic pressure or cavitation effect, the miniature one-way valve is in the reverse shut-off state, preventing the fluid mixture inside the central axial outlet section from flowing back towards the micro pump.

[0046] See attached document Figure 2 The central timing master control and data interconnection system includes a microcontroller chip and peripheral driver circuit boards. The general-purpose input / output pins of the microcontroller chip are electrically connected to the control terminal of the electromagnetic reversing valve in the pneumatic auxiliary system. This connection is used to output level signals to control the air circuit opening and reversing of the micro cylinder, thereby driving the photoacoustic coaxial punching and in-situ marking module to perform linear feed or retraction along the Z-axis.

[0047] The pulse width modulation (PWM) signal output pin of the microcontroller chip is connected to the enable control terminal of an external ultrasonic generator. The external ultrasonic generator is connected to the terminals of the sandwich piezoelectric transducer via wires. When the PWM signal is active, the ultrasonic generator outputs a high-frequency AC signal of a preset frequency to the sandwich piezoelectric transducer, exciting the piezoelectric ceramic to oscillate.

[0048] The microcontroller chip establishes data connections with the stepper motor driver and machine vision barcode reader in the tape-mount package collection system via a serial communication bus. The digital-to-analog converter pin and the specific pulse generation pin of the microcontroller chip are connected to the actuator of the piezoelectric micropump and the constant current drive power supply of the ultraviolet light-emitting diode array, respectively, for precise control of the pump fluid volume and the intensity and duration of ultraviolet light source radiation.

[0049] A complete single-plant sampling and labeling operation cycle is strictly divided into multiple continuous and partially overlapping physical execution time segments under the internal clock control of the microcontroller chip. In the first time segment, the microcontroller chip outputs a positive drive level signal to the electromagnetic directional valve, causing the amplitude transformer to descend and contact the plant leaf. During this descent, the microcontroller chip synchronously outputs an ultrasonic enable signal, causing the annular cutting edge on the end face of the amplitude transformer to vibrate at high frequency, performing physical cutting action and preventing plant sap adhesion.

[0050] During the second timing segment, when the annular cutting edge reaches the set lower Z-axis stop position, the cut plant leaf disc falls into the receiving micro-hole of the base tape. At this time, the microcontroller chip sends a quantitative pulse sequence to the stepper motor driver, driving the base tape and the heat-sealing film to advance synchronously by one hole spacing. After the base tape moves into position, the heat-sealing assembly closes the receiving micro-hole covering the leaf disc. Simultaneously, the microcontroller chip triggers the machine vision reader to perform image acquisition and read the machine-readable QR code data array on the back of the corresponding receiving micro-hole.

[0051] During the third timing phase, the microcontroller chip sends a predetermined number of trigger pulses to the actuator of the piezoelectric micropump. The piezoelectric micropump then pushes a fixed volume of liquid photosensitive resin into the microfluidic channel inside the amplitude transformer and flows to the bottom opening. During this period, the microcontroller chip maintains the ultrasonic generator enabled, utilizing the continuous high-frequency sound field at the end face to allow the injected liquid photosensitive resin to penetrate into the cut surface of the plant leaf.

[0052] During the fourth timing segment, the microcontroller chip deactivates the enable signal of the ultrasonic generator, and the ultrasonic vibration stops. At a predetermined clock cycle, the microcontroller chip sends an on-state signal to the constant current drive power supply, energizing the ultraviolet (UV) LED array. The UV beam illuminates the resin layer at the plant wound site via the internal light guide path of the amplitude transformer. The timer inside the microcontroller chip calculates and maintains this on-state signal for a specific duration based on the critical energy density required for resin curing and the measured output light intensity. Once the resin is fully cross-linked and cured, the power supply to the light source is cut off.

[0053] During the fifth time segment, the microcontroller chip outputs a reverse drive level signal to the electromagnetic reversing valve. The pneumatic micro-cylinder drives the photoacoustic coaxial punching and in-situ marking module to retract upward along the Z-axis to the initial spatial coordinate position, completing the physical closed-loop operation of sampling and in-situ marking of the current single plant.

[0054] At the initial stage of single-plant sampling, the microcontroller chip outputs a positive drive level signal to the control terminal of the electromagnetic reversing valve. A set working pressure difference is established between the intake and exhaust chambers on both sides of the piston inside the pneumatic micro-cylinder, driving the entire photoacoustic coaxial punching and in-situ marking module to move linearly downwards along the Z-axis guide rail. At the set spatial position point during the module's descent, the microcontroller chip outputs a valid signal to the enable control pin of the ultrasonic generator, causing the annular punching blade at the bottom of the through-type ultraviolet light guide ultrasonic amplitude transformer to vibrate, generating axial high-frequency mechanical vibration with a specific frequency and fixed displacement amplitude.

[0055] As the feeding motion continues, the end face of the annular punching blade contacts the surface of the target plant leaf. The static feed pressure provided by the pneumatic miniature cylinder and the dynamic alternating force generated by high-frequency mechanical vibration are applied together to the plant epidermis and mesophyll tissue. When the peak shear stress in the contact area exceeds the mechanical yield limit of the plant fiber structure and vascular bundle tissue, the plant tissue undergoes physical fracture.

[0056] During this cutting process, plant cells rupture, releasing liquid plant sap containing water, macromolecular proteins, nucleic acids, and polysaccharides. The high-frequency mechanical vibration of the annular punch blade generates an alternating acoustic pressure field within the plant sap medium surrounding the blade surface. When the acoustic pressure is in a negative half-cycle and the local pressure is below the saturated vapor pressure of the liquid sap, microscopic cavitation bubbles are generated within the sap. In the subsequent positive half-cycle, the cavitation bubbles collapse under pressure. The microjets and acoustic jets generated at the moment of bubble collapse act on the metal surface boundary layer of the annular punch blade, producing a continuous physical peeling force. This interrupts the intermolecular adhesion process between the organic macromolecules in the plant sap and the metal surface of the blade, thereby maintaining the cleanliness of the annular punch blade's working surface and eliminating material residue correlations between cross-sample operations.

[0057] After the plant mesophyll tissue is completely severed by the annular punch, it forms a physically isolated, independent leaf disc. At the displacement node where this leaf disc is completely separated from the surrounding parent leaf, under the combined action of the mechanical kinetic energy from the photoacoustic coaxial punch and the downward feeding of the in-situ marking module, the leaf disc's own weight, and the axial mechanical repulsive force generated by the high-frequency vibration of the annular punch end face, the leaf disc falls directionally along the vertically penetrating sampling channel inside the sampling support base. The falling leaf disc enters the currently stationary micropore on the bottom belt transmission path directly below.

[0058] When the photoacoustic coaxial punching and in-situ marking module descends along the Z-axis to the physical bottom stop of its mechanical stroke, the stroke sensor mounted next to the slider assembly generates a position arrival electrical signal and transmits this signal to the input port of the microcontroller chip. The microcontroller chip records this timing node as the trigger condition for the completion of a single physical cutting action.

[0059] The microcontroller chip triggers the stepping logic of the tape-based packaging collection system based on the position arrival electrical signal. The microcontroller chip sends a specific number of control pulses to the stepper motor driver. The stepper motor drives the take-up shaft to rotate by a preset angle, pulling the bottom tape and the hot-melt sealing film along a horizontal guide path to move a geometric distance equal to the size of the micro-orifice. During this displacement, the micro-orifice containing the blade enters the working area of ​​the hot-press sealing assembly. The electrically heated roller maintains mechanical pressure contact with the outer surface of the hot-melt sealing film in this area, conducting heat energy to the hot-melt adhesive coating at the bottom of the sealing film, causing a phase change and thermally cross-linking with the material on the upper surface of the bottom tape, thus forming an independent, sealed internal space for the micro-orifice.

[0060] During the stationary time window when the stepper motor completes its displacement and is electrically locked, the microcontroller chip sends an image acquisition trigger level to the machine vision reader. The image sensor inside the machine vision reader opens the electronic shutter, acquiring image data of the machine-readable QR code array positioned on the second surface area of ​​the bottom strip directly above the lens. This image data is decoded and converted into a unique digital sequence identifier, which is temporarily stored in the microcontroller chip's register, thus completing the system mapping between the physical entity of the packaged sample and its digital encoding.

[0061] When the photoacoustic coaxial punching and in-situ marking module reaches the set physical bottom stop along the Z-axis and the annular punch cuts through the plant mesophyll tissue, the microcontroller chip maintains the air inlet pressure of the pneumatic micro-cylinder, ensuring mechanical contact between the end face of the annular punch and the edge of the cut wound on the plant leaf. Simultaneously, the microcontroller chip keeps the ultrasonic generator enabled. Subsequently, the microcontroller chip sends a preset number of electrical pulse signals to the actuator of the piezoelectric micro-pump.

[0062] Driven by an electrical pulse signal, the piezoelectric micro-pump undergoes mechanical deformation, pumping a fixed volume of liquid photosensitive resin from a light-proof reservoir through a second sealed conduit and a miniature one-way valve. The pumped liquid photosensitive resin enters the inclined inlet section of the side wall of the through-type ultraviolet light-conducting ultrasonic amplitude transformer along a flexible delivery tube, and flows into the central axial outlet section. Driven by the mechanical hydrostatic pressure provided by the piezoelectric micro-pump, the fluid moves along the central axial outlet section to the lowest output end face of the through-type ultraviolet light-conducting ultrasonic amplitude transformer, directly making physical contact with the cut surface of the plant leaf within the internal area enclosed by the annular cutting edge.

[0063] Liquid photosensitive resin exhibits non-Newtonian fluid rheological properties. When this fluid comes into contact with the lower end face of a through-type ultraviolet light-conducting ultrasonic amplitude transformer and enters the high-frequency acoustic field region it generates, the high-frequency acoustic field applies alternating mechanical stress to the fluid. Under the continuous shearing action of this high-frequency alternating mechanical stress, the polymer chain segments inside the liquid photosensitive resin undergo a change in orientation, resulting in a significant decrease in its apparent dynamic viscosity with increasing shear rate, exhibiting shear-thinning physical properties. This process transforms the high-viscosity liquid photosensitive resin under normal conditions into a low-viscosity fluid state.

[0064] During the propagation of sound waves from the end face of a through-type ultraviolet light-conducting ultrasonic amplitude transformer through liquid photosensitive resin into plant tissue, the attenuation of sound field energy generates a constant acoustic radiation pressure along the direction of sound wave propagation within the fluid medium. This acoustic radiation pressure induces directional macroscopic fluid motion within the fluid, i.e., acoustic flow. The physical driving force generated by the acoustic flow is vector-superimposed with the mechanical hydrostatic pressure provided by the piezoelectric micropump. Driven by the superimposed composite pressure gradient, the low-viscosity liquid photosensitive resin overcomes the mechanical flow resistance of the capillary pores inside the plant, and undergoes directional fluid penetration into the plant tissue along the exposed vascular bundles and intercellular spaces of the cut surface of the plant leaf.

[0065] After the penetration time window set by the microcontroller chip's internal timer, the liquid photosensitive resin completes spatial filling and distribution in the intercellular spaces of the plant's surface and subsurface layers. The fluid penetrating into the plant tissue maintains physical communication with the resin droplets residing on the plant wound surface, forming a continuous fluid network that permeates the plant's external surface and internal micropores. This continuous fluid network configuration provides a three-dimensional spatial distribution basis for the mechanical interlocking and in-situ solid-state anchoring after the subsequent ultraviolet photopolymerization reaction. After completing the set fluid penetration operation sequence, the microcontroller chip deactivates the ultrasonic generator's enable signal, ultrasonic vibration stops, and the system enters the photocuring execution sequence.

[0066] After the microcontroller chip deactivates the enable signal of the ultrasonic generator, the timing logic inside the microcontroller chip enters a set delay period to ensure that the residual high-frequency mechanical vibration is completely attenuated. After the delay period ends, the microcontroller chip outputs an enable signal to the constant current drive power supply. The ultraviolet light-emitting diode array obtains its rated operating current and emits an initial divergent ultraviolet beam with a wavelength in the range of 365nm to 395nm.

[0067] The initial diverging ultraviolet beam passes sequentially through the aspherical condensing lens and the collimating plano-convex lens in the collimating lens group. Optical refraction alters the propagation path, converting it into a parallel ultraviolet beam. This parallel ultraviolet beam propagates in a straight line through space, passes through the hollow pre-tightening bolt inside the sandwich piezoelectric transducer, and enters the quartz glass or sapphire substrate material through the top input surface of the through-type ultraviolet light guide ultrasonic amplitude transformer.

[0068] A parallel ultraviolet (UV) beam propagates axially downwards within the substrate material. When an edge ray with a certain divergence angle contacts the physical boundary of the outer side of the through-type UV light guide ultrasonic amplitude transformer, total internal reflection occurs due to the refractive index difference between the substrate material and the external air medium. The optical energy of the UV beam is confined within the solid waveguide formed by the substrate material for propagation. After passing through this transmission path, the UV beam exits from the lowest output end face of the through-type UV light guide ultrasonic amplitude transformer and directly irradiates the wound area of ​​the plant leaf and the liquid photosensitive resin medium permeated within the plant tissue.

[0069] The liquid photosensitive resin mixture system contains a specific ratio of photoinitiator, polymerizable monomers, and oligomers. When the number of ultraviolet photons irradiating the resin surface reaches the absorption threshold of the photoinitiator, the photoinitiator molecules in the ground state absorb photon energy and transition to the excited state, undergoing molecular cleavage to generate highly chemically active free radicals or cations. These active species then collide with unsaturated groups such as carbon-carbon double bonds on the monomer or oligomer molecular chains in the resin system, triggering a chain polymerization chemical reaction.

[0070] As the cross-linking polymerization reaction continues throughout the light-irradiated fluid region, the molecular chains within the resin gradually cross-link in three-dimensional space to form a macromolecular network polymer structure. Accompanying the formation of the macromolecular network structure, the macroscopic physical state of the photosensitive resin irreversibly transforms from a liquid fluid phase to a solid polymer phase within a set time window.

[0071] During this photopolymerization phase transition, the resin fluid residing on the plant cut surface and the resin fluid that previously infiltrated the plant's vascular bundles and intercellular spaces via acoustic flow simultaneously undergo cross-linking. After curing, the polymer forms branching entities within the plant's micropores, and these branching entities are covalently linked to the cured main structure covering the plant's outer surface, forming a physically inseparable whole. This three-dimensional structure creates a microscopic level of mechanical interlocking at the plant tissue cut, enabling the solid resin marker patch to establish a rigid, resistant mechanical connection to the target plant tissue.

[0072] The microcontroller chip's internal hardware timer continuously records the output time of the on-level signal. The microcontroller chip compares this recorded time with the target exposure time value, pre-calculated based on the resin's critical crosslinking energy density and the end-face output light intensity. When the recorded time reaches the target exposure time value, the microcontroller chip toggles the state of its output pin from on to off. The constant current drive power supply responds to the off-level, cutting off the current flowing to the ultraviolet light-emitting diode array, stopping ultraviolet beam radiation, and ending the photopolymerization reaction cycle.

[0073] After the beam radiation stops, the microcontroller chip outputs a reverse drive level signal to the electromagnetic reversing valve. The internal air circuit of the pneumatic micro cylinder reverses, driving the piston rod to retract, which in turn drives the photoacoustic coaxial punching and in-situ marking module to retract upward along the Z-axis linear guide, disengaging from the mechanical contact with the solid resin marking patch and plant leaves, and returning to the initial spatial coordinate position, completing a single cycle of physical timing operation.

[0074] In this embodiment of the invention, inorganic fluorescent quantum dot particles are pre-uniformly dispersed in the liquid photosensitive resin mixture encapsulated within the light-proof storage bladder. When excited by an external light source of a set wavelength, the inorganic fluorescent quantum dot particles emit a fluorescence spectrum with a fixed center wavelength and a specific full width at half maximum (FWHM).

[0075] During the time window when the stepper motor of the carrier-based packaging collection system is stationary, the machine vision reader acquires a digital image of the machine-readable QR code array corresponding to the currently accommodating micro-hole on the second surface of the bottom strip. The microcontroller chip receives this digital image and runs its built-in decoding algorithm to convert the image data into a unique string sequence identifier.

[0076] The microcontroller chip has an internal real-time clock circuit. Upon receiving a position arrival signal from the travel sensor, the microcontroller chip reads and latches the current system absolute time parameter. Simultaneously, the microcontroller chip's non-volatile memory pre-programs characteristic spectral data of the inorganic fluorescent quantum dots doped in the current batch of liquid photosensitive resin.

[0077] During the data processing phase of a single sampling and labeling operation cycle, the microcontroller chip extracts the structured data corresponding to the current operation sequence by executing instructions and constructs a multi-dimensional mapping relationship for data traceability. For the system's execution of the first... In the single-plant processing task, the data processing unit of the microcontroller chip encapsulates its associated discrete data into data tuples. The set mapping relationship of this data tuple satisfies the following expression:

[0078] ;

[0079] In the formula, Defined as the first The comprehensive traceability data tuple generated by the single-plant processing task; Defined as the first In the single-plant processing task, the machine vision barcode reader reads and parses the string sequence identifier from the baseband; Defined as the first The absolute timestamp generated when the annular punching blade reaches the physical bottom stop point in the single-plant processing task; Defined as the first The fluorescence emission peak wavelength of the inorganic fluorescent quantum dots contained in the liquid photosensitive resin injected and solidified in situ on the plant during the single-plant treatment task; It is a positive integer representing the sequence number of the task pipeline in continuous operation of the system.

[0080] To ensure the uniqueness and tamper-resistance of the generated data tuples, the microcontroller chip's data processing unit calls a preset cryptographic hash function to perform calculations on the comprehensive traceability data tuples to generate verification ciphertext. The hash operation logic is as follows:

[0081] ;

[0082] In the formula, Defined as for the first A fixed-length check hash value generated from the data of the single-plant processing task; Defined as a selected one-way cryptographic hash algorithm function; symbol Defined as a concatenation operator for data bit strings. After the operation is completed, the microcontroller chip transmits the comprehensive traceability data tuple through an external communication interface. and the corresponding check hash value The data packets are sent to an external central database server for persistent storage.

[0083] Through the aforementioned logical structure and control flow, the digital code corresponding to the plant leaf sample physically isolated within the sealed micropores of the blind box, and the solid resin marker patch containing specific spectral characteristics residing at the cut surface of the plant mother, are logically mapped at the same time point within the physical storage space of the external central database server. In subsequent sample nucleic acid extraction and plant field management, the fluorescence wavelength of the marked patches on the plants in the field is measured by reading the blind box's bottom band code or using a portable spectrometer, and then compared with the data tuples stored within the database server to perform forward or reverse data tracing operations.

[0084] Specific application examples:

[0085] In the field selection stage of high-throughput molecular breeding of wheat, breeders need to sample a large number of wheat plants in the jointing stage for genotyping and reliably mark the retained parent plants.

[0086] Breeders use the integrated device for precise sampling and marking of individual wheat breeding materials, as described in this invention, to align the photoacoustic coaxial punching and in-situ marking module with the target wheat leaf. Upon triggering the central timing control and data interconnection system, the feed drive mechanism pushes the module downwards. At this time, the through-type ultraviolet light guide ultrasonic amplitude transformer outputs high-frequency mechanical vibration at a specific frequency, causing the annular punching blade to cut and separate the wheat leaf mesophyll tissue under the vibration. The cut plant tissue falls directly into the micro-pores of the carrier-type encapsulation and collection system. The heat-sealing assembly then seals it tightly, and a machine vision reader simultaneously reads the machine-readable QR code data array at the bottom of the micro-pores, parsing it into a string sequence identifier.

[0087] At the instant the annular cutting blade maintains contact with the cut surface of the plant leaf, a piezoelectric micropump pumps liquid photosensitive resin doped with inorganic fluorescent quantum dots into the microfluidic channel and reaches the cut surface. The high-frequency alternating mechanical stress output by the amplitude transformer causes the resin to shear thin, and under acoustic flow drive, it deeply penetrates the vascular bundles and intercellular spaces of the wheat. Subsequently, an ultraviolet beam emitted by an ultraviolet light-emitting diode array coaxially penetrates the amplitude transformer, irradiating the resin in the cut area, causing it to undergo a photopolymerization and cross-linking reaction. The cured resin forms a solid resin tag patch with a mechanically interlocking structure in the micropores inside the wheat and on its outer surface. Finally, the system hashes and encrypts the string sequence identifier, absolute timestamp, and quantum dot characteristic spectral data, and uploads it to the central database server, completing the closed-loop operation from physical sampling to digital binding.

[0088] Experimental verification and effect comparison:

[0089] To verify the anti-pollution capability and marker anchoring stability of the device of the present invention in practical applications, a comparative experiment was conducted using wheat breeding populations from the same experimental field.

[0090] The photoacoustic coaxial punching mode and the conventional mechanical punching mode with the ultrasonic function turned off, respectively, were used to continuously sample wheat plants. Every 10 samples, the surface of the annular punching blade was rinsed with deoxyribonucleic acid (DNA) elution buffer, and the concentration of residual DNA in the elution buffer was measured using a fluorescence quantitative spectrophotometer.

[0091] like Figure 3 As shown, in conventional mechanical punching mode, the concentration of residual DNA on the blade surface increases with the number of consecutive samplings, reaching a level that easily leads to false positives by the 50th sampling. Conversely, in the photoacoustic coaxial punching mode of this invention, high-frequency mechanical vibration generates cavitation bubbles in the plant sap medium. The microjets formed by the collapse of these bubbles continuously exert a physical stripping effect, effectively blocking the adhesion of nucleic acid macromolecules to the metal boundary layer. Even after 50 consecutive samplings, the concentration of residual DNA on the blade surface remains at an extremely low baseline level, eliminating the risk of cross-contamination across samples from the hardware level.

[0092] Marking patches were generated on wheat plant leaves using both conventional surface coating marking and the ultrasonic penetration interlocking marking method of this invention. The marked plants were placed in a field environment simulating wind, sun exposure, and rainfall. Every 5 days, a miniature push-pull force gauge was used to test the vertical peel resistance of the marking patches, and the critical tensile peak value at which the patches physically detached was recorded.

[0093] like Figure 4 As shown, conventional surface-coated markers adhere to the plant epidermis solely through intermolecular forces. Their peel strength rapidly diminishes under field stress, and they essentially lose adhesion after 20 days, easily detaching naturally. The ultrasonic-penetrating interlocking markers used in this invention, through shear thinning and acoustic permeation mechanisms, allow the liquid photosensitive resin to penetrate deep into the plant's micropores before photopolymerization. The resulting rigid mechanical interlocking structure after curing gives the patch extremely high initial adhesion. In a 25-day field retention test, its peel strength decreased slowly and remained consistently high, ensuring long-term stable anchoring of the marker throughout the breeding cycle.

Claims

1. An integrated device for precise sampling and labeling of single wheat breeding materials, characterized in that, include: Feed drive mechanism, photoacoustic coaxial punching and in-situ marking module, tape-type packaging and collection system, microfluidic liquid supply and pneumatic auxiliary system, and central timing master control and data interconnection system; The central timing master control and data interconnection system is used to output control commands; The feed drive mechanism is used to respond to the control command and drive the photoacoustic coaxial punching and in-situ marking module to feed toward the target plant tissue; The photoacoustic coaxial punching and in-situ marking module is used to output high-frequency mechanical vibration during the feeding process to cut and separate the target plant tissue, thereby obtaining the cut plant tissue; The carrier-type encapsulation and collection system is used to receive the severed plant tissue and perform sealed encapsulation and string sequence identification reading; The microfluidic liquid supply and pneumatic auxiliary system is used to quantitatively pump liquid photosensitive resin into the photoacoustic coaxial punching and in-situ marking module. The photoacoustic coaxial punching and in-situ marking module is also used to inject the liquid photosensitive resin into the plant cutting wound and coaxially output ultraviolet light beams to irradiate the liquid photosensitive resin to cause a photopolymerization and cross-linking reaction, forming a solid resin marking patch that establishes a logical mapping relationship with the string sequence identifier.

2. The integrated device for precise sampling and labeling of single wheat breeding materials according to claim 1, characterized in that, The photoacoustic coaxial punching and in-situ marking module includes a sandwich piezoelectric transducer, a hollow preload bolt, and an ultraviolet light-emitting diode array substrate assembly. The sandwich piezoelectric transducer has a cylindrical through hole inside, and the hollow pre-tightening bolt is assembled in the cylindrical through hole and forms a coaxial hollow optical path channel inside the hollow pre-tightening bolt. The ultraviolet light-emitting diode array substrate assembly is used to emit the ultraviolet light beam, and the emitted ultraviolet light beam enters and penetrates the entire coaxial hollow optical path channel along the axis.

3. The integrated device for precise sampling and labeling of single wheat breeding materials according to claim 2, characterized in that, The photoacoustic coaxial punching and in-situ marking module also includes a through-type ultraviolet light guide ultrasonic amplitude transformer. The through-type ultraviolet light guide ultrasonic amplitude transformer is used to synchronously transmit the high-frequency mechanical vibration and the ultraviolet beam in the same physical axis by utilizing the matrix material properties of the through-type ultraviolet light guide ultrasonic amplitude transformer. The through-type ultraviolet light guide ultrasonic amplitude transformer has a microfluidic internal flow channel inside. The bottom opening of the central axial liquid outlet section of the microfluidic internal flow channel and the emission area of ​​the ultraviolet beam are both located inside the annular punching blade at the bottom of the through-type ultraviolet light guide ultrasonic amplitude transformer.

4. The integrated device for precise sampling and labeling of single wheat breeding materials according to claim 3, characterized in that, The microfluidic liquid supply and pneumatic auxiliary system includes a light-proof liquid storage bladder and a piezoelectric micropump; The microfluidic internal flow channel includes a sidewall oblique liquid inlet section. The axial spatial coordinate of the inlet of the sidewall oblique liquid inlet section is set on the cross section of the standing wave field distribution propagating along the through-type ultraviolet light guide ultrasonic amplitude transformer. The piezoelectric micropump is used to receive voltage pulse signals and pump the liquid photosensitive resin in the light-proof liquid storage bladder into the microfluidic internal flow channel through the inlet of the sidewall oblique liquid inlet section.

5. The integrated device for precise sampling and labeling of single wheat breeding materials according to claim 4, characterized in that, In the fluid infiltration operation sequence, the through-type ultraviolet light-conducting ultrasonic amplitude transformer is used to output alternating mechanical stress to trigger the physical property of shear thinning in the liquid photosensitive resin, thereby reducing the apparent dynamic viscosity. Simultaneously, it generates acoustic radiation pressure in the fluid medium to induce acoustic flow, driving the liquid photosensitive resin in a low-viscosity state to undergo directional fluid infiltration into the exposed vascular bundles and intercellular spaces of the plant cutting wound. Subsequently, the solid resin label patch is generated by photopolymerization and cross-linking reaction under the irradiation of the ultraviolet light beam.

6. The integrated device for precise sampling and labeling of single wheat breeding materials according to claim 3, characterized in that, During the cutting process, the through-type ultraviolet light guide ultrasonic amplitude rod is used to drive the annular punching blade to maintain a fixed displacement amplitude of high-frequency mechanical vibration. It induces an alternating acoustic pressure field and generates cavitation bubbles in the contact area between the end face of the annular punching blade and the released plant sap medium. The micro-jet generated by the pressure collapse of the cavitation bubbles blocks the intermolecular adhesion of plant organic macromolecules to the boundary layer of the metal surface of the annular punching blade.

7. The integrated device for precise sampling and labeling of single wheat breeding materials according to claim 1, characterized in that, The carrier tape packaging and collection system includes a base tape, a hot melt sealing film, a hot press sealing assembly, and a machine vision barcode reader. The bottom strip is provided with equally spaced micro-holes, and the second surface of the bottom strip is printed with a machine-readable QR code data array. The hot-press sealing assembly is used to thermally cross-link the hot-melt sealing film covering the area above the receiving micropores of the cut plant tissue with the first surface of the base strip, and the machine vision barcode reader is used to acquire an image of the machine-readable QR code data array in the corresponding area and decode it into the string sequence identifier.

8. The integrated device for precise sampling and labeling of single wheat breeding materials according to claim 1, characterized in that, The central timing master control and data interconnection system includes a microcontroller chip. For a single sampling and marking operation cycle, the microcontroller chip is used to sequentially output a positive drive level signal, an ultrasonic enable signal, a control command with a specific number of pulses, an image acquisition trigger level, a preset number of electrical pulse signals, and an on-level signal with a specific duration according to the timing beat.

9. The integrated device for precise sampling and labeling of single wheat breeding materials according to claim 8, characterized in that, The microcontroller chip integrates a hardware timer. The microcontroller chip is also used to calculate the target exposure time value based on the preset resin critical energy density and the pre-measured output light intensity, and to flip the on-level signal to the off level after the continuous output time of the on-level signal reaches the target exposure time value.

10. The integrated device for precise sampling and labeling of single wheat breeding materials according to claim 7, characterized in that, The central timing master control and data interconnection system is used to extract the string sequence identifier generated by the machine vision barcode reader, the absolute timestamp latched when a single cutting action is completed, and the characteristic spectral data of the inorganic fluorescent quantum dots pre-placed in the liquid photosensitive resin in this batch, and combine them to construct a comprehensive traceability data tuple; and further call the one-way encrypted hash algorithm function to perform operations on the comprehensive traceability data tuple to generate a verification hash value, which is used to complete the persistent storage of the logical mapping relationship.

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