Polypeptide complex combination for reinforcing the fight against free radicals for skin anti-aging firming

Abstract

The application belongs to the technical field of cosmetics and skin care, and provides a polypeptide compound combination for strengthening the resistance to free radicals for skin anti-aging and tightening. The application constructs a multi-pathway efficacy system with five active polypeptides as the core, which respectively covers free radical scavenging, stress resistance, anti-glycation / anti-carbonylation, collagen promotion and repair promotion nodes. In the obtained in-vitro and 3D full-thickness skin model data, the product shows multiple synergistic effects such as antioxidant, anti-carbonylation, anti-glycation, collagen promotion, elastin promotion and repair promotion, and can be used in various types and functions of cosmetics.

Description

Technical Field

[0001] This application belongs to the field of cosmetics and skin care technology. Specifically, this application provides a combination of polypeptide complexes for skin anti-aging and firming to enhance the fight against free radicals. Background Technology

[0002] Skin aging is simultaneously affected by oxidative stress, carbonylation, and glycation. Ultraviolet radiation, environmental pollution, and metabolic activities lead to an increase in oxygen, nitrogen, carbon, and sulfur free radicals, which in turn induce lipid peroxidation, collagen degradation, and cell damage. Simultaneously, reducing sugars and reactive carbonyl compounds undergo non-enzymatic reactions with proteins, forming AGEs and causing collagen cross-linking and decreased elasticity. Therefore, to develop a truly competitive anti-aging system, it is essential to consider multi-type free radical protection, anti-glycation / anti-carbonylation, and post-damage repair.

[0003] Existing anti-aging ingredients in cosmetics mainly include vitamin C, vitamin E, coenzyme Q10, peptides, and anti-glycation ingredients. However, these ingredients often have problems such as insufficient free radical scavenging ability, poor transdermal absorption, single pathway of action, and strong irritation. Summary of the Invention

[0004] To address the aforementioned issues, this invention utilizes five active polypeptides—decarboxylated carnosine hydrochloride, hexapeptide-11, acetyl tetrapeptide-2, palmitoyl pentapeptide-4, and hexapeptide-9—as the main functional system, supplemented with tetrahydromethylpyrimidine carboxylic acid and a cosmetically acceptable carrier, to form a complex with antioxidant, anti-glycation, anti-carbonylation, anti-wrinkle, firming, and repairing effects.

[0005] On the one hand, this application provides a polypeptide complex combination for skin anti-aging and firming, which strengthens the fight against free radicals, the polypeptide complex combination comprising decarboxylated carnosine hydrochloride, hexapeptide-11, acetyl tetrapeptide-2, palmitoyl pentapeptide-4, and hexapeptide-9.

[0006] Furthermore, the mass ratio of decarboxylated carnosine hydrochloride, hexapeptide-11, acetyl tetrapeptide-2, palmitoyl pentapeptide-4, and hexapeptide-9 in the polypeptide complex is 40-60: 1-3: 1-3: 1-3: 1-3: 1-3.

[0007] Furthermore, the mass ratio of decarboxylated carnosine hydrochloride, hexapeptide-11, acetyl tetrapeptide-2, palmitoyl pentapeptide-4, and hexapeptide-9 in the polypeptide complex is 45-55:1.3-1.8:1.8-2.2:1.3-1.8:1.8-2.2.

[0008] Furthermore, the mass ratio of decarboxylated carnosine hydrochloride, hexapeptide-11, acetyl tetrapeptide-2, palmitoyl pentapeptide-4, and hexapeptide-9 in the polypeptide complex is 50:1:2:1:2.

[0009] Furthermore, the total content of decarboxylated carnosine hydrochloride, hexapeptide-11, acetyl tetrapeptide-2, palmitoyl pentapeptide-4, and hexapeptide-9 in the polypeptide complex is 0.5-5 w / w.

[0010] Furthermore, the polypeptide complex also contains tetrahydromethylpyrimidine carboxylic acid.

[0011] Furthermore, the polypeptide complex also includes a cosmetically acceptable carrier.

[0012] Furthermore, the acceptable carrier in the cosmetic is selected from one or more of 1,2-hexanediol, 1,2-pentanediol, PEG-40 hydrogenated castor oil, butylene glycol, and water.

[0013] On the other hand, this application provides a method for preparing the above-mentioned polypeptide complex, the method comprising: dissolving a cosmetically acceptable carrier at 40-50°C; adding the polypeptide component and dissolving it after cooling to 25-35°C; homogenizing and filtering at 2000-3000 rpm.

[0014] Furthermore, the method includes dissolving an acceptable carrier in the cosmetic; dissolving the peptide component; homogenizing; and filtering.

[0015] On the other hand, this application provides the application of the above-mentioned polypeptide complex combination in the preparation of cosmetics with antioxidant effects.

[0016] On the other hand, this application provides the application of the above-mentioned polypeptide complex combination in the preparation of cosmetics with repairing effects.

[0017] Furthermore, the cosmetic product is a serum, essence lotion, face cream, emulsion, eye cream, or face mask.

[0018] Furthermore, the amount of the polypeptide complex added in the serum or lotion is 0.5-10 w / w, serving as the core antioxidant and anti-glycation active system; the amount of the polypeptide complex added in the cream or lotion is 0.5-2.0 w / w; the amount of the polypeptide complex added in the eye cream is 0.5-1.0 w / w; and the amount of the polypeptide complex added in the mask is 1.0-3.0 w / w.

[0019] Beneficial effects This application constructs a multi-pathway efficacy system based on five active peptides, covering free radical scavenging, anti-stress, anti-glycation / anti-carbonylation, collagen promotion, and repair promotion. In the obtained in vitro and 3D full-thickness skin model data, the product exhibits multiple synergistic effects such as anti-oxidation, anti-carbonylation, anti-glycation, collagen promotion, elastin promotion, and repair promotion. Detailed Implementation

[0020] Example 1: Basic composition and efficacy of the complex The main components of the complex and their corresponding effects are shown in Table 1: Table 1. Main components of the complex and their corresponding effects

[0021] Decarboxylated carnosine hydrochloride focuses on free radical scavenging, reactive carbonyl compound capture, and anti-glycation, serving as a key component in the antioxidant / anti-glycation chain. Hexapeptide-11 and acetyl tetrapeptide-2 primarily support cellular stress resistance and regeneration, amplifying overall defense and repair effects. Palmitoyl pentapeptide-4 focuses on collagen production, contributing significantly to wrinkle reduction and matrix reconstruction. Hexapeptide-9 focuses on tissue repair and migration enhancement, determining repair performance at low doses. Ectoin primarily provides cell protection and system stabilization support.

[0022] Example 2: Preparation and application of the complex Preparation method: Step 1: Raw material preparation. Weigh each raw material according to the formula, and check the batch number, appearance, expiration date and storage condition.

[0023] Step 2: Aqueous phase preparation. Heat purified water to 40-50°C, then add butanediol, 1,2-pentanediol and 1,2-hexanediol in sequence, stirring until completely dissolved.

[0024] Step 3: Adding solubilizer. Add PEG-40 hydrogenated castor oil and continue stirring until the system is homogeneous and clear.

[0025] Step 4: Addition of active ingredients. Cool the system to 25–35°C and add decarboxylated carnosine hydrochloride, hexapeptide-11, acetyl tetrapeptide-2, palmitoyl pentapeptide-4, hexapeptide-9 and / or tetrahydromethylpyrimidine carboxylic acid.

[0026] Step 5: Homogenization and filtration. Homogenize at a low speed of 2000-3000 rpm for about 10 minutes, and then filter through a 0.45 μm filter membrane.

[0027] Step 6: Quality Inspection and Filling. After testing pH, appearance, microorganisms, and characteristic content, store at 2–8°C in the dark.

[0028] Application method: Serum / Emulsion: The preferred addition amount is 1.0% to 5.0%, as the core antioxidant and anti-glycation active system.

[0029] Face cream / lotion: Preferred addition amount is 0.5-2.0%, for anti-aging, firming and repair.

[0030] Eye cream: The preferred addition amount is 0.5% to 1.0%, used for anti-wrinkle, firming and fine line improvement.

[0031] Facial mask: The preferred addition amount is 1.0% to 3.0%, used for repair and anti-stress.

[0032] Sunscreen products: The preferred addition amount is 0.5% to 1.0%, as an auxiliary antioxidant activity system.

[0033] Example 3 Experimental Scheme The types and amounts of peptides in the experimental composition are shown in Table 2. Other components of the composition are 0.3 w / w% tetrahydromethylpyrimidine carboxylic acid, 1% w / w 1,2-hexanediol, 2 w / w% 1,2-pentanediol, 2 w / w% PEG-40 hydrogenated castor oil, 30% w / w butanediol, and the balance is water.

[0034] Table 2 Experimental Composition Schemes .

[0035] Example 4: Composition Effect Test The efficacy testing was conducted by Shaanxi Boxi General Testing Technology Co., Ltd. (1) Free radical scavenging ability test The test sample was from Example 1; the scavenging ability against carbon-centered and oxygen-centered free radicals was evaluated using DPPH and PTIO models, respectively, and the results are shown in Table 3. Table 3. Results of Free Radical Scavenging Ability Test

[0036] Conclusion: Example 1 showed significant DPPH scavenging activity at concentrations of 0.625% and above; the concentration dependence was more obvious in the PTIO model, with a scavenging rate of 32.98% at 5%.

[0037] (2) 3D full-layer skin model efficacy test The model conditions were UVA irradiation combined with MGO stimulation (irradiation dose 15 J / cm²). 2 The test sample was from Example 1, with a concentration of 1% (v / v). The results are shown in Table 4: Table 4. Results of efficacy test of 3D full-thickness skin model

[0038] Conclusion: Example 1 showed clear activity in anti-carbonylation, anti-glycation, collagen promotion, and elastin promotion, indicating that the system has multi-pathway anti-aging potential.

[0039] (3) Cell repair test The keratinocyte scratch migration model was used, and the test sample was from Example 1, with a concentration of 0.0025% (v / v). The results are shown in Table 5: Table 5. Results of Cell Repair Test

[0040] Conclusion: At extremely low concentrations, Example 1 can increase cell migration by 28%, indicating that it has practical significance for skin repair.

[0041] (4) Safety assessment Cell viability of the fibroblast samples (Example 1) at different concentrations was assessed using the MTT assay. The results are shown in Table 6. Table 6 Safety Assessment Results

[0042] Conclusion: Within the relevant test range, Example 1 did not show significant cytotoxicity and has a good foundation for application safety.

[0043] (5) Comparative verification of synergistic effects The synergistic effects of different composition schemes are shown in Tables 7 and 8.

[0044] Table 7 Synergistic Effects of Different Composition Schemes - 1

[0045] Table 7 Synergistic Effects of Different Composition Regimens - 2

[0046] in conclusion: After removing decarboxylated carnosine hydrochloride, the PTIO scavenging, anti-carbonylation, and anti-glycation indices decreased most significantly, indicating that it is a key fulcrum in the antioxidant / anti-glycation pathway.

[0047] After removing palmitoyl pentapeptide-4, the most significant decreases were observed in type I collagen, type IV collagen, and elastin-related indicators, indicating that it is a crucial factor in anti-wrinkle and matrix reconstruction.

[0048] The most significant decrease in cell migration was observed after the removal of hexapeptide-9, indicating its outstanding contribution to repair and tissue regeneration.

[0049] When two key peptides were removed simultaneously, multiple indicators showed a further collapse-like decline, which can be used to illustrate that the comprehensive effect of the complete pentapeptide system is not a simple superposition of single components.

[0050] Example 5: Application Case The formula for the antioxidant serum is shown in Table 8: Table 8 Antioxidant Serum Formula

[0051] Efficacy: Antioxidant, anti-glycation, anti-wrinkle, firming, and repairing.

Claims

1. A polypeptide complex composition for skin anti-aging and firming, characterized in that, The polypeptide complex comprises decarboxylated carnosine hydrochloride, hexapeptide-11, acetyl tetrapeptide-2, palmitoyl pentapeptide-4, and hexapeptide-9.

2. The polypeptide complex composition according to claim 1, wherein the mass ratio of decarboxylated carnosine hydrochloride, hexapeptide-11, acetyl tetrapeptide-2, palmitoyl pentapeptide-4, and hexapeptide-9 in the polypeptide complex is 40-60:1-3:1-3:1-3:1-3:1-3.

3. The polypeptide complex composition according to claim 2, wherein the mass ratio of decarboxylated carnosine hydrochloride, hexapeptide-11, acetyl tetrapeptide-2, palmitoyl pentapeptide-4, and hexapeptide-9 in the polypeptide complex is 45-55:1.3-1.8:1.8-2.2:1.3-1.8:1.8-2.2, preferably 50:1:2:1:

2.

4. The polypeptide complex combination according to any one of claims 1-3, wherein the total content of decarboxylated carnosine hydrochloride, hexapeptide-11, acetyl tetrapeptide-2, palmitoyl pentapeptide-4, and hexapeptide-9 in the polypeptide complex is 0.5-5 w / w.

5. The polypeptide complex assembly according to claim 1, wherein the polypeptide complex further comprises tetrahydromethylpyrimidine carboxylic acid.

6. The polypeptide complex assembly according to claim 1, wherein the polypeptide complex further comprises a cosmetically acceptable carrier.

7. The polypeptide complex combination according to claim 6, wherein the acceptable carrier in the cosmetic is selected from one or more of 1,2-hexanediol, 1,2-pentanediol, PEG-40 hydrogenated castor oil, butylene glycol, and water.

8. The use of the polypeptide complex combination according to any one of claims 1-7 in the preparation of cosmetics with antioxidant effects.

9. The use of the polypeptide complex combination according to any one of claims 1-7 in the preparation of cosmetics with repairing effects.

10. The application according to claim 8 or 9, wherein the cosmetic is an essence, serum, cream, lotion, eye cream, or mask.

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