Hydrolysis targeting chimera based on artificial antibody and preparation method and application thereof

By coupling artificial gold antibodies and lysosomal sorting signal motif peptides to the surface of gold nanoparticles, a hydrolysis-targeting chimera is formed, solving the problems of size, linkage, and stability of targeted protein degradation drugs, and achieving efficient targeted binding and cell degradation.

CN122404558APending Publication Date: 2026-07-17SHANGHAI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI UNIV
Filing Date
2026-03-17
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing targeted protein degradation drugs suffer from problems such as poor pharmacokinetics due to their large size, difficulty in linking, high development costs, poor stability, and difficulty in controlling the alignment of peptides and nanoparticles.

Method used

Artificial gold antibodies and molecules that can activate cellular degradation mechanisms are coupled to the surface of gold nanoparticles to form a hydrolysis-targeting chimera. Targeted degradation is achieved by the artificial gold antibody binding to the target protein and activating lysosomal sorting signal motif-mediated endocytosis.

Benefits of technology

It improves the affinity and stability of target protein binding, reduces development difficulty, decreases drug size, enhances pharmacokinetic characteristics, and avoids the destruction of binding conformation and uncontrollable orientation.

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Abstract

The present application relates to a kind of hydrolysis targeting chimera based on artificial antibody and its preparation method and application.The hydrolysis targeting chimera is composed of artificial gold antibody and molecule that can activate cell degradation mechanism, the artificial gold antibody is composed of gold nanoparticle and polypeptide coupled on the surface of gold nanoparticle, the molecule that can activate cell degradation mechanism is coupled on the surface of gold nanoparticle.The hydrolysis targeting chimera can simultaneously bind target protein and activate cell degradation mechanism, with the performance of strong specific recognition target protein, and high stability, orientation controllable.At the same time, the hydrolysis targeting chimera can also play the molecule of activation cell degradation mechanism, such as lysosome sorting signal motif, coupled on the surface, induce clathrin-mediated endocytosis, transport the complex of target protein and chimera to lysosome and degrade.The hydrolysis targeting chimera can play multi-mode cancer treatment effect by synergistic inhibition of tumor cell proliferation, invasion pathway and apoptosis activation.
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Citation Information

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