A method for evaluating toxicity of cosmetic raw materials based on co-culture of hair follicle stem cells and endothelial cells

Abstract

The present application is suitable for the field of biological detection and cosmetic safety evaluation technology, and provides a cosmetic raw material toxicity evaluation method based on co-culture of hair follicle stem cells and endothelial cells, which comprises the following steps: separating hair follicle stem cells and endothelial cells from mammalian skin tissue; establishing a non-contact co-culture system of hair follicle stem cells and endothelial cells by using a Transwell chamber, so as to simulate the intercellular interaction in the microenvironment of hair follicle stem cells; applying the cosmetic raw material to be tested to the co-culture system at a gradient concentration; detecting six indexes in six dimensions, i.e., cell viability, cell apoptosis, stem cell marker expression, endothelial barrier function, inflammatory factor release and genetic damage; calculating a comprehensive toxicity score according to a weighted comprehensive toxicity index model and determining a toxicity grade. The present application significantly improves the sensitivity and physiological relevance of toxicity detection, and provides a high-throughput, multi-index and standardized in-vitro alternative method for safety evaluation of cosmetic raw materials.

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