High-selenium compound powder for postoperative and radiotherapy and chemotherapy rehabilitation immune repair and preparation method thereof

Abstract

The application provides a preparation method of high-selenium compound powder for postoperative and radiotherapy rehabilitation immune repair, and belongs to the technical field of functional food or special medical use formula food processing technology.The application realizes sufficient wall breaking by respectively pre-wetting and low-temperature complex enzymolysis of hard plant wall and fungal wall raw materials, and then prevents active loss by using endogenous substances of the raw materials for in-situ anchoring without adding, after drying and crushing, adding postbiotic powder, active peptide, whey protein, yeast beta-glucan, EGCG and other heat-sensitive components into low-temperature sterile dry mixing, while maximizing the release of organic selenium and immune active substances, the heat-sensitive functional components are completely protected from degradation and inactivation, and finally the technical target of sufficient wall breaking, complete activity and stable immune repair effect is achieved.The product prepared by the application can improve the immune organ damage and immune homeostasis imbalance caused by radiotherapy, and is suitable for the immune repair of postoperative and radiotherapy rehabilitation population.

Description

Technical Field

[0001] This invention relates to the field of functional food or special medical purpose formula food processing technology, specifically to a high selenium compound powder for postoperative and radiotherapy / chemotherapy rehabilitation and immune repair, and its preparation method. Background Technology

[0002] In existing technologies, cell wall disruption techniques such as enzymatic hydrolysis, fermentation, and ultrafine grinding are commonly used to improve the dissolution rate of nutrients in plant- or fungal-derived raw materials. However, when the formulation contains both plant / fungal raw materials requiring cell wall disruption and heat-sensitive active ingredients, the conventional one-pot processing method suffers from issues of cell wall disruption efficiency and uniformity, as well as the inactivation of heat-sensitive active ingredients. This results in significant differences in the content of effective active ingredients between different batches of products, making the immunomodulatory effect uncontrollable and failing to meet quality control requirements.

[0003] Therefore, there is an urgent need to develop a new preparation process that can simultaneously achieve efficient cell wall disruption and full retention of thermosensitive activity, in order to resolve the contradiction that existing technologies cannot simultaneously achieve cell wall disruption and activity retention. Summary of the Invention

[0004] To resolve the contradiction between achieving both cell wall disruption and preservation of activity, this invention provides a high-selenium composite powder for postoperative and radiotherapy / chemotherapy rehabilitation and immune repair, along with its preparation method. This method achieves thorough cell wall disruption by pre-wetting and low-temperature enzymatic hydrolysis of hard plant and fungal cell wall materials. Then, endogenous substances from the raw materials are used for additive-free in-situ anchoring to prevent activity loss. After drying and pulverization, heat-sensitive components such as post-biotics, active peptides, whey protein, β-glucan, and EGCG are added under low-temperature aseptic conditions. This maximizes the release of organic selenium and immune-active substances while completely protecting the heat-sensitive functional components from degradation and inactivation, ultimately achieving the technical goal of thorough cell wall disruption, intact activity, and stable immune repair effects.

[0005] One objective of this invention is to provide a method for preparing a high-selenium compound powder for postoperative and radiotherapy / chemotherapy rehabilitation and immune repair, comprising: S1. Mix high-selenium corn germ powder, selenium-enriched broccoli powder, puffed corn powder, corn powder and Chinese yam powder with the first wetting liquid, with a material-to-liquid ratio of 1:(2.5~3.5), and wet at 2~6℃ for 8~18 minutes to obtain the first wetting material; Selenium-enriched black fungus powder and selenium-enriched monkey head mushroom powder are mixed with the second wetting liquid at a ratio of 1:(1.5~2.5) and wetted at 2~6℃ for 3~12 minutes to obtain the second wetting material. The first wetting solution is a sterile deionized aqueous solution with a pH of 7.8 to 8.2; The second wetting solution is a sterile deionized aqueous solution with a pH of 6.8 to 7.2; S2. Combine the first wetting material and the second wetting material, add a compound enzyme preparation to carry out an enzymatic hydrolysis reaction, and obtain the enzymatic hydrolysate. The compound enzyme preparation includes cellulase and neutral protease; S3. Cool the enzymatically hydrolyzed material to 2-6°C and stir continuously at this temperature for 15-30 minutes to obtain the anchoring material; S4. Dry the anchoring material to a moisture content of ≤5%, and pulverize it under a protective atmosphere to a powder particle size D90 ≤20μm to obtain an enzymatically hydrolyzed cell wall breaking base material; S5. The enzymatically hydrolyzed cell wall breaking base material is dry-mixed with the thermosensitive active raw material group to obtain the high selenium composite powder; The thermosensitive active ingredient group includes at least one of epigenetic powder, corn oligopeptide powder, pea peptide powder, concentrated whey protein powder, yeast β-glucan, epigallocatechin gallate, and polydextrose.

[0006] Preferably, the raw materials, by weight, include: 20-28 parts of high-selenium corn germ powder; 4-6 parts of selenium-enriched black fungus powder; 4-6 parts of selenium-enriched broccoli powder; 4-6 parts of selenium-enriched monkey head mushroom powder; 8-12 parts puffed corn flour; 8-12 parts cornmeal; 4-6 parts of Chinese yam powder; 8-15 parts of post-natal vitamin powder; 5-10 parts corn oligopeptide powder; 5-10 parts of pea peptide powder; 4-8 parts concentrated whey protein powder; 1.5 to 3 parts of yeast β-glucan; 1-2 parts of epigallocatechin gallate; Polydextrose 2-5 parts.

[0007] Preferably, in step S2, the cellulase has an enzyme activity of 50,000 to 200,000 U / g; and the neutral protease has an enzyme activity of 50,000 to 200,000 U / g.

[0008] Preferably, in step S2, the mass ratio of the cellulase to the neutral protease is 1:(0.8~1.2).

[0009] Preferably, in step S2, the mass ratio of the cellulase to the neutral protease is 1:1.

[0010] Preferably, in step S2, the total amount of the compound enzyme preparation added is 0.6% to 1.5% of the total mass of the high-selenium corn germ powder, selenium-enriched broccoli powder, puffed corn powder, corn powder, Chinese yam powder, selenium-enriched black fungus powder and selenium-enriched monkey head mushroom powder.

[0011] Preferably, in step S2, the conditions for the enzymatic hydrolysis reaction are: temperature 38~48℃, pH value 6.5~7.5, and time 45~120 minutes; The preferred temperatures are 42-45℃, pH 6.8-7.2, and time 60-90 minutes.

[0012] Preferably, the drying is specifically gradient drying, including: Pre-freezing: Pre-freeze at -60℃ to -50℃ for 3 to 5 hours; Vacuum freeze drying: freeze-dry at -45℃ to -35℃ and a vacuum of 10~50Pa for 10~15 hours; Low-temperature microwave drying: Under the condition that the material temperature is ≤45℃, microwave dry for 20~40 minutes until the material moisture content is ≤5%.

[0013] Preferably, in step S5, the dry mixing is carried out under a protective atmosphere; The dry mixing conditions are a temperature of 15~20℃, a relative humidity of ≤30%, and a nitrogen protective atmosphere.

[0014] Preferably, in the thermosensitive active raw material group: The postbiotic powder is IOBPRO® probiotic segmented solid-state fermented postbiotic powder. The concentrated whey protein powder has a protein content of ≥70%, and osteopontin accounts for ≥90% of the total protein.

[0015] The second objective of this invention is to provide a high-selenium composite powder for postoperative and radiotherapy / chemotherapy rehabilitation and immune repair.

[0016] The beneficial effects of this invention are: This invention utilizes pre-wetting and low-temperature enzymatic hydrolysis of hard plant and fungal cell wall materials to fully break down cell walls of high-selenium corn germ, selenium-enriched black fungus, selenium-enriched broccoli powder, and selenium-enriched lion's mane mushroom powder, thereby increasing the dissolution rate of organic selenium. Simultaneously, heat-sensitive substances such as post-biotics, active peptides, whey protein, yeast β-glucan, and EGCG are dry-mixed afterward, without undergoing wetting, enzymatic hydrolysis, or wet heat treatment throughout the entire process, thus avoiding activity degradation and inactivation.

[0017] By utilizing the plant proteins, fungal polysaccharides, pectin and other endogenous substances contained in the raw materials, selenoproteins, selenopolysaccharides and active peptides are anchored in situ at low temperatures. Without adding any exogenous stabilizers, antioxidants and wall materials, the migration, loss and oxidation of active ingredients can be inhibited, making the product's immune activity more stable.

[0018] After being ultra-finely pulverized at low temperature under nitrogen protection, the product has uniform particle size, good solubility, and high absorption efficiency. It is gentle and safe, without causing gastrointestinal irritation or burden, and can be used for long-term nutritional intervention and dietary supplementation for people who are recovering from surgery, radiotherapy and chemotherapy, or have immune organ damage and lymphocyte function suppression.

[0019] The high-selenium compound powder produced can increase the spleen index and thymus index, effectively improve the atrophy of immune organs caused by chemotherapy drugs such as cyclophosphamide; enhance DTH delayed-type hypersensitivity reaction and HC50 serum hemolysin levels, and simultaneously strengthen cellular immunity and humoral immunity; stably upregulate IL-2 and IL-6, downregulate abnormally elevated TNF-α and IFN-γ, and repair the imbalance of immune factor network; histopathological observation of spleen tissue shows that lymphocytes are more densely arranged and the tissue structure is closer to that of the normal group, and the effect of immune homeostasis repair is clear and reliable. Attached Figure Description

[0020] Figure 1 This is a bar chart showing the effect of the high-selenium composite powder prepared in Example 1 of the present invention on the spleen index of immunocompromised mice. Figure 2 The bar chart shows the effect of the high-selenium composite powder prepared in Example 1 of this invention on the thymus index of immunocompromised mice. Figure 3 HE-stained pathological micrographs of spleen tissue from various experimental mice in this invention; Figure 4 A bar chart showing the effect of the high-selenium composite powder prepared in Example 1 of the present invention on the degree of toe swelling in immunocompromised mice with delayed-type hypersensitivity. Figure 5 The bar chart shows the effect of the high-selenium composite powder prepared in Example 1 of this invention on the serum half-hemolytic value (HC50) of immunocompromised mice. Figure 6 A bar chart showing the effect of the high-selenium composite powder prepared in Example 1 of the present invention on the phagocytic index of macrophages in immunocompromised mice; Figure 7 The bar chart shows the effect of the high-selenium composite powder prepared in Example 1 of this invention on the serum interleukin-2 (IL-2) content of immunocompromised mice. Figure 8 The bar chart shows the effect of the high-selenium composite powder prepared in Example 1 of this invention on the serum interleukin-6 (IL-6) content of immunocompromised mice. Figure 9 The bar chart shows the effect of the high-selenium composite powder prepared in Example 1 of this invention on the serum tumor necrosis factor-α (TNF-α) content in immunocompromised mice. Figure 10This is a bar chart showing the effect of the high-selenium composite powder prepared in Example 1 of the present invention on the serum interferon-γ (IFN-γ) content of immunocompromised mice. Detailed Implementation

[0021] The present application will now be described in further detail with reference to embodiments. In the following description, certain specific details are included to provide a comprehensive understanding of the various disclosed embodiments. However, those skilled in the art will recognize that embodiments can be implemented without employing one or more of these specific details, but using other methods, components, materials, etc. Unless otherwise required by the present invention, the terms "comprising" and "including" should be interpreted in an open-ended, inclusive sense, meaning "including but not limited to". Throughout this specification, "an embodiment," "an embodiment," "a preferred embodiment," or "some embodiments" means that at least one embodiment includes a specific reference element, structure, or feature related to that embodiment. Therefore, the phrases "in an embodiment," "in an embodiment," "in a preferred embodiment," or "in some embodiments" appearing in different places throughout the specification do not necessarily all refer to the same embodiment. Furthermore, specific elements, structures, or features may be combined in one or more embodiments in any suitable manner.

[0022] According to a first aspect of the present invention, a method for preparing a high-selenium composite powder for postoperative and radiotherapy / chemotherapy rehabilitation and immune repair is provided, comprising: S1. Mix high-selenium corn germ powder, selenium-enriched broccoli powder, puffed corn powder, corn powder and Chinese yam powder with the first wetting liquid, with a material-to-liquid ratio of 1:(2.5~3.5), and wet at 2~6℃ for 8~18 minutes to obtain the first wetting material; Selenium-enriched black fungus powder and selenium-enriched monkey head mushroom powder are mixed with the second wetting liquid at a ratio of 1:(1.5~2.5) and wetted at 2~6℃ for 3~12 minutes to obtain the second wetting material. The first wetting solution is a sterile deionized aqueous solution with a pH of 7.8 to 8.2; The second wetting solution is a sterile deionized aqueous solution with a pH of 6.8 to 7.2; S2. Combine the first wetting material and the second wetting material, add a compound enzyme preparation to carry out an enzymatic hydrolysis reaction, and obtain the enzymatic hydrolysate. The compound enzyme preparation includes cellulase and neutral protease; S3. Cool the enzymatically hydrolyzed material to 2-6°C and stir continuously at this temperature for 15-30 minutes to obtain the anchoring material; S4. Dry the anchoring material to a moisture content of ≤5%, and pulverize it under a protective atmosphere to a powder particle size D90 ≤20μm to obtain an enzymatically hydrolyzed cell wall breaking base material; S5. The enzymatically hydrolyzed cell wall breaking base material is dry-mixed with the thermosensitive active raw material group to obtain the high selenium composite powder; The thermosensitive active ingredient group includes at least one of epigenetic powder, corn oligopeptide powder, pea peptide powder, concentrated whey protein powder, yeast β-glucan, epigallocatechin gallate, and polydextrose.

[0023] In this invention, high-selenium corn germ powder, selenium-enriched broccoli powder, puffed corn powder, corn powder, and Chinese yam powder are raw materials for hard plant cell walls. The cell walls are tough and have a high cellulose content. Therefore, a slightly alkaline first wetting solution with a higher material-to-liquid ratio and a longer wetting time is required to achieve sufficient softening and lay the foundation for subsequent uniform cell wall breaking. Selenium-enriched black fungus powder and selenium-enriched monkey head mushroom powder are raw materials for soft fungal cell walls. The cell walls are loose and have a high polysaccharide content. They are prone to adhesion and inactivation under slightly alkaline conditions. Therefore, a neutral second wetting solution with a lower material-to-liquid ratio and a shorter wetting time is used to soften the fungal cells while protecting the integrity of the fungal polysaccharide structure.

[0024] By using differentiated wetting, we can solve common industry problems such as uneven cell wall disruption, incomplete enzymatic hydrolysis, and easy loss of activity in multi-raw material systems.

[0025] Using a single enzyme alone cannot simultaneously break down plant and fungal cell walls, easily leading to insufficient release of organic selenium and selenium polysaccharides. Cellulase can efficiently disrupt the cellulose skeleton of plant and fungal cell walls, opening up their dense structures; neutral protease can gently loosen structural proteins in the raw materials, promoting the full dissolution of selenoproteins and bioactive peptides; the two work synergistically under neutral and mild conditions, achieving sufficient cell wall disruption without damaging key active substances such as organic selenium, selenium polysaccharides, and yam polysaccharides, ensuring the maximum release of immune-active substances.

[0026] The selenoproteins, selenopolysaccharides, and active peptides released by enzymatic hydrolysis are thermosensitive, easily migrated, and easily oxidized. Conventional processes often result in significant loss and inactivation of these components during drying. The in-situ association and anchoring method described in this invention refers to the spontaneous formation of a stable three-dimensional molecular network within the enzymatically hydrolyzed material system, without the addition of any exogenous stabilizers or antioxidants. This network relies solely on the material's own endogenous proteins, polysaccharides, and pectin as a natural framework. Under low temperature (2-6°C) and gentle stirring, a stable three-dimensional molecular network is spontaneously formed through three intermolecular forces: hydrogen bonding, electrostatic adsorption, and hydrophobic interactions. This network encapsulates, adsorbs, and immobilizes the free selenoproteins, selenopolysaccharides, and active peptides, thereby inhibiting the migration, volatilization, and oxidation of these active ingredients and improving the product's activity retention rate and functional stability.

[0027] Post-operative patients and those undergoing radiotherapy and chemotherapy have fragile gastrointestinal function, requiring powders that are fine, easily soluble, and easily absorbed. Meanwhile, components such as selenoproteins and fungal polysaccharides are heat-sensitive substances, and high temperatures and oxygen can cause irreversible inactivation. Controlling the moisture content to ≤5% ensures the product is less prone to mold growth and has a longer shelf life. Pulverizing to a D90 ≤20μm under a protective atmosphere avoids overheating and oxidation during pulverization, while significantly improving solubility, dispersibility, and gastrointestinal absorption efficiency, reducing the digestive burden on immunocompromised individuals.

[0028] In a preferred embodiment of the present invention, to achieve the best organic selenium compounding effect and immune synergy, and to ensure the feasibility of the process, the main selenium-rich raw materials used in the present invention meet the following quality standards: The total selenium content of the high-selenium corn germ powder is 10~50 mg / kg, of which organic selenium accounts for ≥90%, and the main form is selenomethionine. The total selenium content of the selenium-enriched broccoli powder is 5~30 mg / kg, of which organic selenium accounts for ≥85%, and the main forms are selenomethionine and selenomethylselenocysteine. The total selenium content of the selenium-enriched black fungus powder is 5~25 mg / kg, of which organic selenium accounts for ≥90%, and the main forms are selenium polysaccharides and selenium proteins. The total selenium content of the selenium-enriched Hericium erinaceus powder is 5~25 mg / kg, of which organic selenium accounts for ≥90%, and the main forms are selenium polysaccharides and selenium proteins.

[0029] All of the above-mentioned raw materials can be purchased commercially or obtained using known plant / fungal selenium-enriched culture techniques. Using raw materials that meet or exceed the above standards can achieve the technical effects of this invention.

[0030] The epigenic powder, corn oligopeptides, pea peptides, whey protein concentrate, yeast beta-glucan, EGCG, and polydextrose in the formula are all high-value heat-sensitive / enzyme-sensitive active substances. If they are subjected to moist heat treatments such as wetting, enzymatic hydrolysis, and drying, they will be degraded and inactivated, leading to a significant decrease in their immune synergistic effects. Therefore, they are added dry after being pulverized, ensuring that they do not come into contact with water, enzymes, or heat throughout the process, thus preserving their original activity.

[0031] In a preferred embodiment of the present invention, the raw materials, by weight parts, include: 20-28 parts of high-selenium corn germ powder; 4-6 parts of selenium-enriched black fungus powder; 4-6 parts of selenium-enriched broccoli powder; 4-6 parts of selenium-enriched monkey head mushroom powder; 8-12 parts puffed corn flour; 8-12 parts cornmeal; 4-6 parts of Chinese yam powder; 8-15 parts of post-natal vitamin powder; 5-10 parts corn oligopeptide powder; 5-10 parts of pea peptide powder; 4-8 parts concentrated whey protein powder; 1.5 to 3 parts of yeast β-glucan; 1-2 parts of epigallocatechin gallate; Polydextrose 2-5 parts.

[0032] In this invention, the high-selenium corn germ powder is primarily a source of organic selenium. Selenium exists in the form of selenoproteins and selenopolysaccharides, and is a key substance for enhancing the body's immune function and repairing immune cell damage. The dosage is set at 20-28 parts to ensure sufficient and safe organic selenium content in the product. This meets the high-efficiency selenium supplementation needs of immunocompromised individuals after chemotherapy without posing safety risks due to excessive selenium content, while also providing sufficient plant protein carriers for endogenous anchoring. Selenium-enriched black fungus powder, selenium-enriched broccoli powder, and selenium-enriched monkey head mushroom powder are all selenium-enriched medicinal and edible raw materials, rich in fungal polysaccharides, sulforaphane precursors, dietary fiber, and organic selenium. Together with the high-selenium corn germ powder, they form a multi-source selenium complex system, synergistically enhancing immune regulation, antioxidant effects, and intestinal protection. The dosage is controlled at 4-6 parts to ensure a synergistic immune effect while avoiding excessive fungal raw materials that could lead to powder moisture absorption, clumping, and decreased flowability, thus ensuring stability during subsequent processing and reconstitution.

[0033] Puffed corn flour, used as a carrier and mouthfeel modifier, can improve powder formability, flowability, and reconstitution properties, enhance product palatability, and reduce the swallowing and gastrointestinal burden on postoperative / chemoradiotherapy patients. It also provides mild carbohydrates for rapid energy replenishment. Using 8-12 parts ensures sufficient carrier action without diluting the concentration of active ingredients and preventing a decrease in functional efficacy. Chinese yam powder contains yam polysaccharides, mucoproteins, and other components, offering mild spleen-strengthening and stomach-nourishing effects, protecting the gastrointestinal mucosa, and assisting in immune regulation. It is particularly suitable for patients with weakened gastrointestinal function after chemotherapy. Using 4-6 parts can provide gastrointestinal protection and immune support, while also providing endogenous pectin to help stabilize the active ingredients.

[0034] Post-biotics are fermentation products of *Lactobacillus paracasei*, which can regulate intestinal flora, strengthen the intestinal barrier, and activate mucosal immunity. Together with organic selenium, they form an immune-intestinal dual-axis synergistic repair system, significantly improving intestinal and immune damage caused by chemotherapy. A dosage of 8-15 parts ensures significant immunomodulatory effects while avoiding excessive additions that would increase costs and cause unpleasant powder odors.

[0035] Both corn oligopeptide powder and pea peptide powder are small-molecule active peptides that are easily absorbed and do not increase the burden on the gastrointestinal tract. They can directly participate in the repair of immune cells, enhance lymphocyte activity, and synergistically exert antioxidant effects with selenium. A dosage of 5-10 parts can achieve efficient absorption and immune enhancement. At the same time, as an endogenous anchored polypeptide component, it stabilizes the activity of selenoproteins and polysaccharides.

[0036] Concentrated whey protein powder is rich in whey protein and osteopontin. Osteopontin can promote the proliferation and activation of immune cells, enhancing the body's anti-infection and immune repair capabilities. A dosage of 4-8 parts is sufficient to ensure immune repair function, while avoiding excessive protein that could lead to an overly fishy smell and reduced solubility.

[0037] Yeast β-glucan is a potent immune activator that can activate macrophages and enhance cellular immune function, forming a triple immune synergy with organic selenium and post-biotics. A dosage of 1.5 to 3 parts is sufficient to achieve a significant immune-enhancing effect; excessive addition can easily cause powder adhesion, reduced flowability, and affect processing performance.

[0038] Epigallocatechin gallate (EGCG) is a powerful antioxidant that can scavenge excess free radicals produced by chemotherapy, reduce oxidative stress damage, and protect immune cells. A dosage of 1-2 parts is sufficient for high-efficiency antioxidant effects without affecting product stability; avoid excessive dosage as it can lead to an overly bitter taste and powder discoloration.

[0039] Polydextrose is a water-soluble dietary fiber that can regulate intestinal function, promote the proliferation of beneficial bacteria, and improve constipation, while also enhancing the solubility and stability of the powder. A dosage of 2-5 servings can provide both intestinal protection and process assistance, without causing bloating, making it suitable for people with weakened constitutions.

[0040] In a preferred embodiment of the present invention, in step S2, the cellulase has an enzyme activity of 50,000 to 200,000 U / g; the neutral protease has an enzyme activity of 50,000 to 200,000 U / g.

[0041] In this invention, the neutral protease includes at least one of Neutrase® neutral protease manufactured by Novozymes, Amjad® neutral protease NP-100a / NP-5, or Borl brand neutral protease NP-2000.

[0042] In a preferred embodiment of the present invention, in step S2, the mass ratio of the cellulase to the neutral protease is 1:(0.8~1.2).

[0043] In this invention, plant materials are based on cellulose and hemicellulose, which require cellulase to fully break them down; fungal materials and plant materials both contain a large number of structural proteins and selenoproteins, which require gentle dissolution by neutral proteases to promote the dissolution of organic selenium, selenium polysaccharides and active peptides.

[0044] By controlling the mass ratio of the two to a near-equal proportion of 1:(0.8~1.2), a dynamic balance can be achieved between the cellulase's disruptive effect on the cell wall skeleton and the neutral protease's loosening effect on the protein matrix. This avoids both excessive polysaccharide degradation due to an excessively high proportion of cellulase and excessive protein hydrolysis and flavor degradation due to an excessively high proportion of neutral protease. As a result, the four types of selenium-enriched raw materials can be broken down simultaneously, uniformly, and gently, maximizing the release of organic selenium and immune-active substances.

[0045] In a preferred embodiment of the present invention, in step S2, the mass ratio of the cellulase to the neutral protease is 1:1.

[0046] In this invention, within the range of 1:(0.8~1.2), 1:1 is the optimal synergistic balance point for this formulation. It can simultaneously meet the high-efficiency cell wall disruption requirements of both plant and fungal cells, achieving optimal cell wall disruption uniformity; it minimizes the damage to selenoproteins, selenopolysaccharides, yam polysaccharides, and fungal polysaccharides, resulting in the highest activity retention rate; the degree of enzymatic hydrolysis is moderate, ensuring sufficient dissolution of water-soluble active ingredients without excessive liquefaction of the system, which is beneficial for the stable execution of subsequent endogenous anchoring, drying, and pulverization processes. Experiments have shown that the product prepared at this ratio exhibits the best overall performance in terms of organic selenium dissolution rate, immune activity, and powder stability. Therefore, 1:1 has been determined as the optimal ratio.

[0047] In a preferred embodiment of the present invention, in step S2, the total amount of the compound enzyme preparation added is 0.6 to 1.5% of the total mass of the high-selenium corn germ powder, selenium-enriched broccoli powder, puffed corn powder, corn powder, Chinese yam powder, selenium-enriched black fungus powder and selenium-enriched monkey head mushroom powder.

[0048] In this invention, when the added amount is less than 0.6%, the total activity of cellulase and neutral protease is insufficient to simultaneously break down the cell walls of both hard plant cells and soft fungi, resulting in incomplete cell wall disruption and low dissolution rates of organic selenium and selenium polysaccharides. This fails to achieve the purpose of enhancing immune efficacy, leading to insufficient product function. Adding an amount exceeding 1.5% leads to excessive enzymatic hydrolysis: on the one hand, it causes excessive hydrolysis of active substances such as selenoproteins, fungal polysaccharides, and yam polysaccharides, significantly reducing immune activity; on the other hand, it makes the material system too thin and viscous, hindering subsequent endogenous anchoring, drying, and pulverization, while also producing bitterness and off-flavors, affecting palatability and making it unsuitable for postoperative patients and those recovering from radiotherapy and chemotherapy. Within the range of 0.6% to 1.5%, the compound enzyme can simultaneously break down the cell walls of both plants and fungi, ensuring the full release of organic selenium, selenium polysaccharides, and water-soluble active ingredients without degrading or destroying the active substances, thus achieving a balance between cell wall disruption enhancement and activity retention.

[0049] In a preferred embodiment of the present invention, in step S2, the conditions for the enzymatic hydrolysis reaction are: temperature 38~48℃, pH value 6.5~7.5, and time 45~120 minutes. The preferred temperatures are 42-45℃, pH 6.8-7.2, and time 60-90 minutes.

[0050] In this invention, 38-48℃ is the common high-efficiency enzymatic hydrolysis temperature range for food-grade cellulase and neutral protease. Within this temperature range, both enzymes exhibit high activity and stable function, synergistically breaking down plant and fungal cell walls. Below 38℃, enzyme activity significantly decreases, resulting in insufficient cell wall disruption, prolonged processing time, and low production efficiency. Above 48℃, enzyme proteins are prone to denaturation and inactivation, while heat-sensitive substances such as selenium polysaccharides, fungal polysaccharides, and yam polysaccharides begin to degrade, affecting immune activity. 42-45℃ is the golden temperature range where both enzymes exhibit the highest activity, best stability, and minimal damage to the activity of raw materials, achieving an optimal balance between cell wall disruption efficiency and activity retention.

[0051] A neutral environment with a pH of 6.5–7.5 is the optimal pH range for the combined use of cellulase and neutral protease, ensuring that both enzymes function synchronously and avoiding inhibition of one enzyme or uneven cell wall disruption due to acidic / alkaline conditions. A pH < 6.5 or pH > 7.5 significantly reduces enzymatic hydrolysis efficiency and damages selenoprotein structures, fungal polysaccharides, and sulforaphane precursors, leading to activity loss. A pH of 6.8–7.2 is the mild range closest to the natural pH of the raw material system, preserving the organic selenium form, maintaining the endogenous anchoring effect, and not interfering with subsequent drying and pulverization processes.

[0052] If the reaction time is less than 45 minutes, the enzymatic hydrolysis is insufficient, the cell walls are not completely broken, and the organic selenium and selenium polysaccharides are not fully dissolved, resulting in a weak immune effect. If the reaction time is more than 120 minutes, excessive enzymatic hydrolysis will occur, leading to the degradation of active substances, stickiness of materials, deterioration of flavor, and a prolonged production cycle. The optimal time is 60-90 minutes, which ensures that the four types of selenium-enriched raw materials are simultaneously and fully broken down, while avoiding the activity decay, flavor deterioration, and increased energy consumption caused by prolonged processing. This represents the optimal balance between industrial production and functional effects.

[0053] In a preferred embodiment of the present invention, step S4 specifically involves gradient drying, including: Pre-freezing: Pre-freeze at -60℃ to -50℃ for 3 to 5 hours; Vacuum freeze drying: freeze-dry at -45℃ to -35℃ and a vacuum of 10~50Pa for 10~15 hours; Low-temperature microwave drying: Under the condition that the material temperature does not exceed 45℃, microwave dry for 20~40 minutes until the material moisture content is ≤5%.

[0054] Single freeze-drying methods are time-consuming, costly, and incomplete in dehydration; single microwave drying methods are prone to overheating, deactivation, clumping, and poor taste. In this invention, a three-stage gradient drying process of pre-freezing, vacuum freeze-drying, and low-temperature microwave drying is adopted instead of a single drying method to achieve the optimal balance between thorough dehydration, structural fixation, activity protection, and ensuring powder quality.

[0055] Pre-freezing at -60℃ to -50℃ for 3-5 hours rapidly forms uniform, fine ice crystals, preventing large ice crystals from piercing cell walls and protecting the structural integrity of active substances such as selenoproteins, selenopolysaccharides, and endogenous polysaccharides. Sufficient pre-freezing prevents bubbling, collapse, shrinkage, and overflow of materials under vacuum, ensuring structural stability after enzymatic hydrolysis and facilitating continuous production. Freezing above -50℃ is slow and prone to forming large ice crystals; freezing below -60℃ is too energy-intensive and economical; 3-5 hours ensures complete freezing and uniform internal and external freezing of the material.

[0056] Vacuum freeze drying is carried out entirely under low temperature and negative pressure, without heating or contact with oxygen, thus protecting heat-sensitive materials to the greatest extent. After sublimation and dehydration, the material is in a porous and fluffy state, which facilitates subsequent pulverization, resulting in a final powder with good solubility, no clumping when mixed, and a delicate texture, making it suitable for consumption by people with weak constitutions.

[0057] -45℃ to -35℃ ensures stable sublimation of ice crystals; 10 to 50 Pa is a commonly used high-efficiency vacuum range for food freeze drying; 10 to 15 hours ensures complete removal of free moisture, preventing mold growth caused by intermediate moisture.

[0058] Vacuum freeze drying cannot completely remove bound water and capillary water. Residual moisture can cause powder to absorb moisture, clump, and shorten shelf life. Microwave drying can deeply, quickly, and uniformly dehydrate. Strict temperature control ≤45℃ avoids heat damage. The entire process uses low-temperature microwaves, and the material temperature does not exceed 45℃, which does not destroy activity, does not produce Maillard reactions, does not change color, and does not produce odors, ensuring that the product is mild and palatable.

[0059] Dehydration time below 20 minutes is insufficient; time above 40 minutes can easily lead to local overheating; a final moisture content of ≤5% can improve the flowability and dispersibility of the powder, prevent powder clumping and agglomeration, ensure the uniformity of subsequent dry mixing of heat-sensitive active raw materials, and improve the reconstitution and solubility of the finished product; enhance the storage stability of the product, inhibit the oxidative degradation and activity decay of functional ingredients such as selenoproteins, selenopolysaccharides, and active peptides; extend the shelf life of the product when stored at room temperature in a sealed container, avoid problems such as mold, rancidity, and flavor deterioration caused by excessive moisture, and ensure that the product maintains consistent quality and stable efficacy throughout the entire storage period.

[0060] In a preferred embodiment of the present invention, in step S5, the dry mixing is carried out under a protective atmosphere; The dry mixing conditions are a temperature of 15~20℃, a relative humidity of ≤30%, and a nitrogen protective atmosphere.

[0061] In this invention, EGCG, selenoproteins, active peptides, and functional proteins are all easily oxidized substances. Under aerobic conditions, they rapidly undergo oxidative degradation and activity decline, leading to powder discoloration and flavor deterioration. Nitrogen, being an inert gas, blocks oxidation reactions, fully preserving the immune activity of all active ingredients. The functional metabolites and structural integrity of inactivated bacteria of the metabiotic are easily destroyed under aerobic conditions. A nitrogen atmosphere stabilizes the intestinal barrier regulation and mucosal immune activation effects of the metabiotic, ensuring that its synergistic immune effect with organic selenium is not diminished. The inert nitrogen atmosphere significantly inhibits the growth of microorganisms such as molds and aerobic bacteria, reducing the risk of microbial contamination and meeting the stringent food safety requirements for immunocompromised individuals after surgery or chemotherapy / radiotherapy.

[0062] At the same time, it forms a full-process inert protection with the nitrogen-protected ultrafine grinding process in step S4, avoiding secondary oxidation of the powder during process switching and dry mixing, and achieving continuous activity protection throughout the process.

[0063] In this invention, metagenic elements, osteopontin, small molecule active peptides, and EGCG are prone to protein denaturation and irreversible degradation of active ingredients when the temperature exceeds 20°C; when the temperature is below 15°C, condensation and condensation easily occur in the dry mixing environment, leading to the powder absorbing moisture and clumping. 15~20°C is the golden temperature range that balances the protection of heat-sensitive active ingredients with the prevention of condensation and clumping.

[0064] This temperature perfectly matches the powder temperature after nitrogen ultrafine grinding in step S4, with no significant temperature difference. This prevents the powder from condensing and absorbing moisture, thus reducing its fluidity. It ensures uniform mixing of the enzymatically hydrolyzed cell wall-breaking base material and the heat-sensitive active ingredients, avoiding batch-to-batch variations in active ingredient content. The mild room temperature range does not damage the powder's loose, porous structure, preserving the product's excellent solubility, smooth texture, and lack of gastrointestinal irritation, making it suitable for post-operative patients.

[0065] In this invention, the active peptides, polydextrose, yeast β-glucan, and post-biotic powder in the formula are all highly hygroscopic materials. When the relative humidity of the environment exceeds 30%, they are extremely prone to absorbing moisture and clumping, leading to uneven mixing, clumping during reconstitution, decreased fluidity, and even mold growth during storage. A low-humidity environment of ≤30% can prevent the powder from absorbing moisture at the source, ensuring the stability of the product's processing and edibility.

[0066] Environmental moisture accelerates the hydrolysis of selenoproteins, EGCG oxidation, denaturation of functional proteins, and loss of post-biotic activity. A low-humidity environment (≤30%) can prevent moisture from damaging active ingredients, ensuring the product's immune-repairing efficacy remains stable and controllable throughout the entire storage period. Strictly controlling environmental humidity prevents moisture from entering the powder during dry mixing, ensuring the final product's moisture content remains consistently ≤5%, further improving storage stability and extending shelf life at room temperature. Simultaneously, a low-humidity environment further inhibits microbial growth, creating a synergistic effect with nitrogen protection, providing double protection for the product's food safety.

[0067] In this invention, a mild dry-mixed environment with low temperature, low humidity, and no oxygen is constructed by the synergistic effect of a temperature of 15~20℃, relative humidity ≤30%, and nitrogen protective atmosphere.

[0068] In a preferred embodiment of the present invention, the thermosensitive active raw material group includes: The postbiotic powder is IOBPRO® probiotic segmented solid-state fermented postbiotic powder. The concentrated whey protein powder contains osteopontin.

[0069] In this invention, the concentrated whey protein powder has a protein content of ≥70%, of which osteopontin accounts for no less than 90% of the total protein, and meets EFSA or equivalent food safety standards. Preferably, Lacprodan® OPN-10 is produced by Arla Foods Ingredients, Denmark, which has a protein content of approximately 78%, of which osteopontin accounts for approximately 95% of the total protein.

[0070] In a preferred embodiment of the present invention, the preparation method of the high-selenium composite powder for postoperative and radiotherapy / chemotherapy rehabilitation and immune repair specifically includes: I. Raw Material Formula By weight fraction: 20-28 parts high-selenium corn germ powder; 4-6 parts selenium-enriched black fungus powder; 4-6 parts selenium-enriched broccoli powder; 4-6 parts selenium-enriched lion's mane mushroom powder; 8-12 parts puffed corn flour; 8-12 parts corn flour; 4-6 parts Chinese yam powder; 8-15 parts IOBPRO® probiotics fermented in a segmented solid state; 5-10 parts corn oligopeptide powder; 5-10 parts pea peptide powder; 4-8 parts concentrated whey protein powder containing osteopontin; 1.5-3 parts yeast β-glucan; 1-2 parts epigallocatechin gallate; 2-5 parts polydextrose.

[0071] II. Specific preparation steps S1, graded targeted pre-wetting Under aseptic conditions, the raw materials were weighed according to the formula weight parts, and the raw materials were divided into two groups: a hard plant wall group and a soft fungal wall group, which were then subjected to moistening treatment separately. The first wetting solution is added to the hard plant wall group (high-selenium corn germ powder, selenium-enriched broccoli powder, puffed corn powder, corn powder, and Chinese yam powder), and the material-to-liquid ratio is controlled at 1:(2.5~3.5). After stirring and mixing evenly, the mixture is allowed to stand and wet for 8~18 minutes at a constant temperature of 2~6℃ to obtain the first wetted material. The first wetting solution is a sterile deionized aqueous solution with the pH adjusted to 7.8~8.2 using food-grade sodium hydroxide. A second wetting solution is added to the soft fungal wall group (selenium-enriched black fungus powder, selenium-enriched monkey head mushroom powder), and the material-to-liquid ratio is controlled at 1:(1.5~2.5). After stirring and mixing evenly, the mixture is allowed to stand and wet for 3~12 minutes under constant temperature of 2~6℃ to obtain the second wetted material. The second wetting solution is a sterile deionized aqueous solution with pH adjusted to 6.8~7.2 using food-grade phosphoric acid or food-grade sodium hydroxide.

[0072] S2, Low-temperature compound enzymatic hydrolysis to break cell walls The first wetting material and the second wetting material obtained in step S1 are combined and stirred evenly. A compound enzyme preparation is added to the mixture and stirred evenly before enzymatic hydrolysis is carried out to obtain the enzymatic hydrolysate. The compound enzyme preparation is composed of cellulase and neutral protease, wherein the enzyme activity of cellulase is 50,000 to 200,000 U / g, the enzyme activity of neutral protease is 50,000 to 200,000 U / g, and the mass ratio of cellulase to neutral protease is 1:(0.8 to 1.2), preferably 1:1. The total amount of the compound enzyme preparation added is 0.6-1.5% of the total mass of the hard plant wall tissue and soft fungal wall tissue raw materials; The conditions for the enzymatic hydrolysis reaction are: system temperature 38~48℃, system pH 6.5~7.5, low-speed stirring (50~80 r / min), and reaction time 45~120 minutes; the preferred enzymatic hydrolysis conditions are: system temperature 42~45℃, system pH 6.8~7.2, and reaction time 60~90 minutes; during the enzymatic hydrolysis process, food-grade phosphoric acid or food-grade sodium hydroxide is used to adjust the system pH in real time to keep it stable within the set range.

[0073] S3, Low-temperature intrinsic anchoring After the enzymatic hydrolysis reaction is completed, the hydrolyzed material is immediately cooled rapidly to 2-6°C. Under this constant temperature condition, it is continuously stirred at a speed of 30-60 r / min for 15-30 minutes. The endogenous plant proteins, fungal polysaccharides, pectin and other substances contained in the raw materials are used to associate and anchor the selenoproteins, selenopolysaccharides and active peptides released by the enzymatic hydrolysis in situ through hydrogen bonding, hydrophobic interaction and electrostatic adsorption to obtain anchored materials. The entire process is carried out in a sterile and closed environment without the addition of any exogenous stabilizers, antioxidants and wall materials.

[0074] S4, Gradient drying and nitrogen-protected ultrafine grinding S4.1. The anchoring material obtained in step S3 is subjected to a three-stage gradient drying process until the final moisture content of the material is ≤5%, specifically including: S4.1.1 Pre-freezing: Spread the anchoring material flat in the freeze-drying tray, with a material thickness of ≤10mm, and put it into the freeze dryer. Pre-freeze at -60℃ to -50℃ for 3~5 hours until the material is completely frozen. S4.1.2 Vacuum freeze drying: After pre-freezing, control the cold trap temperature of the freeze dryer to -45℃ to -35℃ and the vacuum degree to 10~50Pa, and freeze dry in vacuum for 10~15 hours to remove free moisture from the material; S4.1.3 Low-temperature microwave drying: After freeze-drying, the material is transferred to a low-temperature microwave drying equipment. The material temperature is controlled to not exceed 45℃. Microwave drying is carried out for 20~40 minutes until the moisture content of the material is ≤5%, and the dried material is obtained. S4.2 Nitrogen-protected ultrafine grinding: The dried material is fed into the ultrafine grinder and ultrafine grinding is carried out under the conditions of nitrogen protection atmosphere (oxygen content ≤2%) and material temperature ≤15℃. The powder is ground to a particle size D90≤20μm to obtain enzymatically hydrolyzed cell wall breaking base material. The entire process is carried out in a closed, low-temperature, and oxygen-free environment.

[0075] S5, Low-temperature anaerobic aseptic dry mixing In a sterile, sealed environment with a temperature of 15-20℃, relative humidity ≤30%, and nitrogen protection, the enzymatically hydrolyzed cell wall-breaking base material obtained in step S4 and the thermosensitive active raw material group are added to a three-dimensional motion mixer according to the formula weight parts, and mixed at a speed of 15-25 r / min for 20-40 minutes until the mixing uniformity RSD ≤3%, to obtain the high selenium composite powder; after mixing, it is immediately sealed and packaged in a nitrogen-protected sterile environment; The thermosensitive active ingredient group includes IOBPRO® probiotics after segmented solid-state fermentation, corn oligopeptide powder, pea peptide powder, concentrated whey protein powder containing osteopontin, yeast β-glucan, epigallocatechin gallate, and polydextrose.

[0076] The above preparation method, through the synergistic design of raw material diversion, graded cell wall breaking, endogenous anchoring, low-temperature oxygen-free protection throughout the process and post-dry mixing of thermosensitive active ingredients, maximizes the release of immunologically active substances from selenium-rich raw materials while fully preserving the bioactivity of thermosensitive functional ingredients, ultimately producing a high-selenium composite powder that meets the needs of the target population.

[0077] According to a second aspect of the present invention, a high-selenium composite powder for postoperative and radiotherapy / chemotherapy rehabilitation and immune repair is provided.

[0078] To verify the feasibility of the preparation method described in this invention, the rationality of the range of each process parameter, and the superior immune repair effect of the product of this invention compared with the prior art, this invention sets up a series of examples and targeted comparative examples. The raw materials of all examples are within the weight parts range of the claims, and the core formulation basis is shown in Table 1: Table 1

[0079] Example 1 S1, graded targeted pre-wetting Hard plant wall material: high selenium corn germ powder, selenium-enriched broccoli powder, puffed corn powder, corn powder, and Chinese yam powder are added to sterile deionized water with pH 8.0 at a material-to-liquid ratio of 1:3 and wetted at 4℃ for 12 minutes to obtain the first wetted material. Soft fungal wall group: selenium-enriched black fungus powder and selenium-enriched monkey head mushroom powder are added to sterile deionized water with pH 7.0, with a material-to-liquid ratio of 1:2, and wetted at 4℃ for 8 minutes to obtain the second wetted material. S2, Low-temperature compound enzymatic hydrolysis to break cell walls The two groups of wetted materials were combined, and a compound enzyme preparation (cellulase: neutral protease = 1:1, both with enzyme activity of 100,000 U / g) was added. The total amount added was 1.0% of the total mass of the raw materials in the enzymatic hydrolysis group. Enzymatic hydrolysis conditions: temperature 43℃, pH 7.0, low-speed stirring 60 r / min, time 75 min; S3, Low-temperature intrinsic anchoring Immediately after enzymatic hydrolysis, the temperature was lowered to 4°C, and the mixture was stirred at 40 r / min for 20 min to obtain the anchoring material. S4, Gradient drying and nitrogen-protected ultrafine grinding Pre-freezing: Pre-freeze at -55℃ for 4 hours; Vacuum freeze drying: -40℃, vacuum degree 30Pa, freeze drying for 12h; Low-temperature microwave drying: Material temperature ≤40℃, microwave drying for 30 minutes, until moisture content ≤5%; Nitrogen-protected ultrafine grinding: oxygen content ≤2%, temperature ≤15℃, grinding to D90=15μm, to obtain enzymatically hydrolyzed cell wall breaking material; S5, Low-temperature anaerobic aseptic dry mixing Environmental conditions: Temperature 18℃, relative humidity 25%, nitrogen protection; The enzymatically hydrolyzed cell wall-breaking base material is mixed with all the heat-sensitive active raw materials at 20 r / min for 30 min. The mixing uniformity RSD is ≤3%. The mixture is then sealed in nitrogen to obtain the finished product.

[0080] Example 2 S1, graded targeted pre-wetting Hard plant wall assembly: Add sterile deionized water with pH 7.8, material-to-liquid ratio 1:2.5, wet at 2℃ for 8 minutes to obtain the first wetted material; Soft fungal wall assembly: Add sterile deionized water with pH 6.8, material-to-liquid ratio 1:1.5, wet at 2℃ for 3 min to obtain the second wetted material; S2, Low-temperature compound enzymatic hydrolysis to break cell walls Compound enzyme preparation: cellulase: neutral protease = 1:0.8, enzyme activity of each is 50,000 U / g, and the total addition amount is 0.6% of the total mass of the raw materials in the enzymatic hydrolysis group; Enzymatic hydrolysis conditions: temperature 38℃, pH 6.5, time 45 min; S3, Low-temperature intrinsic anchoring Cool to 2℃, stir at 30r / min for 15min to obtain anchoring material; S4, Gradient drying and nitrogen-protected ultrafine grinding Pre-freezing: Pre-freeze at -60℃ for 3 hours; Vacuum freeze drying: -45℃, vacuum degree 10Pa, freeze drying for 10h; Low-temperature microwave drying: Material temperature ≤45℃, microwave drying for 20 minutes, until moisture content ≤5%; The material was pulverized to a D90 of 20 μm to obtain an enzymatically hydrolyzed cell wall-breaking material. S5, Low-temperature anaerobic aseptic dry mixing Environmental conditions: temperature 15℃, relative humidity 30%, nitrogen protection; Mix for 20 minutes, then pack with nitrogen to obtain the finished product.

[0081] Example 3 S1, graded targeted pre-wetting Hard plant wall assembly: Add sterile deionized water with pH 8.2, material-to-liquid ratio 1:3.5, wet at 6℃ for 18 min to obtain the first wetted material; Soft fungal wall assembly: Add sterile deionized water with pH 7.2, material-to-liquid ratio 1:2.5, wet at 6℃ for 12 min to obtain the second wetted material; S2, Low-temperature compound enzymatic hydrolysis to break cell walls Compound enzyme preparation: cellulase: neutral protease = 1:1.2, enzyme activity of each is 200,000 U / g, and the total addition amount is 1.5% of the total mass of the raw materials in the enzymatic hydrolysis group; Enzymatic hydrolysis conditions: temperature 48℃, pH 7.5, time 120 min; S3, Low-temperature intrinsic anchoring Cool to 6℃ and stir at 60r / min for 30min to obtain anchoring material; S4, Gradient drying and nitrogen-protected ultrafine grinding Pre-freezing: Pre-freeze at -50℃ for 5 hours; Vacuum freeze drying: -35℃, vacuum degree 50Pa, freeze drying for 15h; Low-temperature microwave drying: Material temperature ≤45℃, microwave drying for 40 minutes, until moisture content ≤5%; The material was pulverized to D90=18μm to obtain the enzymatically hydrolyzed cell wall-breaking material; S5, Low-temperature anaerobic aseptic dry mixing Environmental conditions: Temperature 20℃, relative humidity 28%, nitrogen protection; Mix for 40 minutes, then pack with nitrogen to obtain the finished product.

[0082] Comparative Example 1 Weigh all the raw materials according to the formula weight parts of Example 1, combine them into one group, stir and mix evenly to obtain the whole raw material mixture; Add a sterile deionized water solution with pH 7.0 to the whole raw material mixture, control the material-to-liquid ratio to 1:3, stir evenly, and let it stand at 4℃ for 10 minutes to wet the material. Add the same compound enzyme preparation as in Example 1 (cellulase: neutral protease = 1:1, the total addition amount is 1.0% of the total mass of the raw material mixture) to the wetted material, stir evenly, and carry out the enzymatic hydrolysis reaction according to the S2 enzymatic hydrolysis parameters of Example 1 to obtain the enzymatic hydrolysate. Following the parameters of step S3 in Example 1, the enzymatically hydrolyzed material was subjected to low-temperature endogenous anchoring to obtain the anchored material. Following the parameters of step S4 in Example 1, the anchoring material was subjected to gradient drying and nitrogen-protected ultrafine pulverization. After pulverization, it was directly sealed and packaged with nitrogen to obtain the finished product of Comparative Example 1.

[0083] Comparative Example 2 According to the formula weight parts of Example 1, weigh all the raw materials of the enzymatic hydrolysis group and the thermosensitive active raw material group respectively. Store the two groups of raw materials separately. The thermosensitive active raw material group does not participate in the front-end process. Mix all the raw materials of the enzymatic hydrolysis group evenly, add sterile deionized water solution with pH 7.5, control the material-to-liquid ratio at 1:3, stir evenly, and let stand at 4℃ for 10 minutes to wet the material. Add the same compound enzyme preparation as in Example 1 to the wetted material, and complete the enzymatic hydrolysis reaction according to the S2 enzymatic hydrolysis parameters of Example 1 to obtain the enzymatic hydrolysate. The preparation was then completed in sequence according to the steps of S3 low-temperature internal anchoring, S4 gradient drying and nitrogen-protected ultrafine pulverization, and S5 post-dry mixing of heat-sensitive raw materials in Example 1. The product was then sealed and packaged with nitrogen to obtain the finished product of Comparative Example 2.

[0084] Comparative Example 3 According to the formula weight parts of Example 1, weigh out the enzymatic hydrolysis group raw materials and the thermosensitive active raw material group respectively, and store the two groups of raw materials separately. The thermosensitive active raw material group does not participate in the front-end wetting and enzymatic hydrolysis process. According to the parameters of step S1 in Example 1, the graded targeted prewetting of the hard plant wall group and the soft fungal wall group was completed, and the first wetting material and the second wetting material were obtained by merging them. According to the parameters of step S2 in Example 1, a compound enzyme preparation was added to the combined wetted material to complete the low-temperature compound enzymatic hydrolysis reaction and obtain the enzymatic hydrolysate. After the enzymatic hydrolysis reaction is completed, the internal anchoring operation of cooling and low-temperature constant temperature stirring is not performed. Instead, the enzymatically hydrolyzed material is directly sent to the subsequent drying process. According to the parameters of step S4 in Example 1, the material was subjected to gradient drying and nitrogen-protected ultrafine pulverization to obtain enzymatically hydrolyzed cell wall breaking material. Following the parameters of step S5 in Example 1, the enzymatically hydrolyzed cell wall-breaking base material and the thermosensitive active raw material group were subjected to low-temperature anaerobic aseptic dry mixing, nitrogen-filled and sealed packaging to obtain the finished product of Comparative Example 3.

[0085] Comparative Example 4 According to the formula weight parts of Example 1, weigh out the enzymatic hydrolysis group raw materials and the thermosensitive active raw material group respectively, and store the two groups of raw materials separately. The thermosensitive active raw material group does not participate in the front-end wetting and enzymatic hydrolysis process. According to the parameters of step S1 in Example 1, the graded targeted prewetting of the hard plant wall group and the soft fungal wall group was completed, and the first wetting material and the second wetting material were obtained by merging them. According to the parameters of step S2 in Example 1, a compound enzyme preparation was added to the combined wetted material to complete the low-temperature compound enzymatic hydrolysis reaction and obtain the enzymatic hydrolysate. Following the parameters of step S3 in Example 1, the enzymatically hydrolyzed material was subjected to low-temperature endogenous anchoring to obtain the anchored material. The three-stage gradient drying operation was cancelled. The anchored material was spread flat on the hot air drying tray with a material thickness of ≤10mm and sent into the hot air drying box. It was dried at 60℃ and under normal pressure ventilation conditions until the material moisture content was ≤5% to obtain the dried material. The dried material was subjected to ultra-fine grinding under nitrogen protection according to the ultra-fine grinding parameters in step S4 of Example 1 to obtain enzymatically hydrolyzed cell wall breaking material. Following the parameters of step S5 in Example 1, the enzymatically hydrolyzed cell wall-breaking base material and the thermosensitive active raw material group were subjected to low-temperature anaerobic aseptic dry mixing, nitrogen-filled and sealed packaging to obtain the finished product of Comparative Example 4.

[0086] Performance testing Organic selenium dissolution rate: The proportion of organic selenium dissolved in simulated gastrointestinal fluid was detected according to GB / T 5009.93-2017 "Determination of Selenium in Food". Retention rate of active ingredients: High performance liquid chromatography was used to detect the retention ratio of active ingredients in the finished product relative to the amount of raw materials fed. Angle of repose of powder: Detected using the fixed funnel method; the smaller the angle of repose, the better the powder flowability. The test results are shown in Table 2: Table 2

[0087] The organic selenium dissolution rate of Examples 1-3 was consistently above 87%, with the optimal Example 1 reaching as high as 92.3%, achieving maximum release and efficient utilization of organic selenium from selenium-rich raw materials. Comparative Example 2 showed an organic selenium dissolution rate of only 72.3%, far lower than the Example group, directly demonstrating the necessity of the differentiated wetting technique for hard plant wall and soft fungal wall raw materials in step S1 of this invention: by adapting the pH value, material-to-liquid ratio, and wetting time to the cell wall structures of the two types of raw materials, uniform softening of the raw material cell walls was achieved, laying the foundation for simultaneous and thorough cell wall disruption during subsequent enzymatic hydrolysis. Comparative Example 3 showed an organic selenium dissolution rate of only 68.5%. This is because the low-temperature treatment at 2-6℃ in step S3 of this invention plays a crucial role in rapidly terminating the enzymatic hydrolysis reaction. Comparative Example 3 did not undergo low-temperature isothermal treatment, and the enzymatic hydrolysis reaction could not be terminated in time. Over-enzymatic hydrolysis occurred during the subsequent drying and heating process, leading to the hydrolysis and destruction of active selenium components such as selenoproteins and selenopolysaccharides, resulting in a significant decrease in the proportion of absorbable organic selenium. Comparative Examples 1 and 4 showed organic selenium dissolution rates of only 76.1% and 63.2%, respectively, further demonstrating that the low-temperature complex enzymatic hydrolysis of cellulase and neutral protease in step S2 of this invention, combined with graded pre-wetting, forms a synergistic effect, which can simultaneously break down the cell walls of plants and fungi, maximizing the release of core immune-active substances such as organic selenium and selenium polysaccharides. In contrast, high-temperature hot air drying will destroy the active structure of selenoproteins, further reducing the effective dissolution of organic selenium.

[0088] In Examples 1-3, the initial retention rates of post-active components, EGCG, and osteopontin were consistently above 89%, with the retention rate of the core thermosensitive components in Example 1 exceeding 94%. In Comparative Example 1, the retention rates of post-active components and osteopontin were only 42.3% and 35.6%, respectively, less than half that of the Example group. This completely avoids the pre-wetting and enzymatic hydrolysis processes for easily degradable and inactivated thermosensitive / enzyme-sensitive components, resolving the contradiction between the need for a humid and hot environment for cell wall breaking and the sensitivity of thermosensitive active ingredients to humid and hot environments. In Comparative Example 4, the retention rates of post-active components, EGCG, and osteopontin were only 58.2%, 63.3%, and 49.7%, respectively, far lower than those in the Example group. This demonstrates that the three-stage gradient drying process of pre-freezing, vacuum freeze-drying, and low-temperature microwave drying in step S4 of this invention, operating in a low-temperature, oxygen-free environment throughout, can completely avoid irreversible damage to thermosensitive active ingredients caused by high temperatures, forming a closed-loop process for overall preservation with the subsequent dry-mixing process.

[0089] Comparative Examples 2 and 3 only lacked the graded wetting and endogenous anchoring steps, but retained the post-dry mixing and gradient drying process of the heat-sensitive raw materials. Therefore, the initial retention rates of post-genes and osteopontin in these examples were close to those in the Example Group, further verifying that post-dry mixing of the heat-sensitive raw materials is the key to the survival of heat-sensitive components. The process steps of this invention have clear division of labor and synergistic effect.

[0090] The angle of repose of the powder (the smaller the angle of repose, the better the powder flowability) and the final moisture content directly determine the product's suitability for industrial processing, its reconstitution and edibility, and its basic stability during storage, while also meeting the dietary needs of post-operative and chemotherapy / radiotherapy patients. The powder angles of repose in Examples 1-3 are all ≤28.1°, exhibiting excellent flowability; the final moisture content is stably controlled at 3.2%~3.8%, far exceeding the ≤5% quality control threshold, ensuring comprehensive compliance with powder processing performance and quality stability standards. All comparative examples show powder angles of repose >33°, with significantly weaker flowability than the example group. The core reason is that the graded pre-wetting of this invention avoids excessive water absorption and adhesion of fungal raw materials, and the three-stage gradient drying forms a loose and porous powder structure, fundamentally improving the powder's flowability and dispersibility. This ensures the uniformity of subsequent dry mixing processes and gives the finished product excellent reconstitution and solubility, without burdening the gastrointestinal tract, perfectly meeting the dietary needs of post-operative and chemotherapy / radiotherapy recovery patients. The final moisture content of Comparative Examples 1-4 all exceeded 4.8%, with Comparative Example 4 reaching as high as 6.1%, exceeding the quality control requirements of this invention. This directly proves that the gradient drying process of this invention can achieve precise control of moisture content, while conventional hot air drying is prone to problems such as surface crusting and internal moisture residue, which not only leads to a decrease in powder flowability, but also creates hidden dangers for mold growth, microbial reproduction, and degradation of active ingredients during storage.

[0091] To verify the stability of the core active ingredients and shelf life of the high-selenium composite powder prepared by this invention during the storage period, to verify the key role of the low-temperature endogenous anchoring and full-process low-temperature anaerobic protection process of this invention in the long-term quality and activity retention of the product, and to compare the impact of different preparation processes on the storage stability of the product, this invention adopts the temperature and humidity accelerated stability test method recognized in the food and health food industry. All the finished products of the examples and comparative examples were placed in a temperature and humidity constant environment of 37°C and 75% relative humidity for 3 months of sealed accelerated storage (this accelerated condition is equivalent to 12 months of sealed storage at room temperature). After the storage period, the retention rate of the core active ingredients and microbial indicators of each group of samples were detected.

[0092] The retention rate of organic selenium was determined by hydride atomic fluorescence spectrometry according to GB 5009.93-2017; the retention rate of postbiotic activity was determined by ELISA for the content of key metabolites; the retention rate of EGCG was determined by high performance liquid chromatography (HPLC); the retention rate of osteopontin was determined by enzyme-linked immunosorbent assay (ELISA); and the total bacterial count was determined by plate counting method according to GB 4789.2-2022. The test results are shown in Table 3. Table 3

[0093] After 3 months of accelerated storage, the retention rates of organic selenium, post-biotics, EGCG, and osteopontin in Examples 1-3 remained stable at over 90%, with no significant activity attenuation, achieving stable efficacy throughout the entire storage cycle.

[0094] The significant decrease in the retention rate of active ingredients after acceleration in Comparative Example 3 directly proves that the endogenous anchoring at low temperature stirring at 2~6℃ in step S3 of this invention can achieve in-situ association and fixation of selenoproteins and active peptides released by enzyme decomposition through endogenous plant proteins, fungal polysaccharides, and pectin in the raw materials. Without the addition of any exogenous stabilizers, the oxidation, hydrolysis, and migration of active ingredients during storage can be inhibited from the source, solving the pain point of rapid decline of active ingredients during shelf life in the industry.

[0095] After acceleration, the retention rate of thermosensitive active ingredients in Comparative Examples 1 and 4 was less than 45%, showing a sharp drop compared to the Example Group. This further proves the synergistic effect of the low temperature, oxygen-free, and low humidity protection throughout the entire process of the present invention: from the post-dry mixing of thermosensitive raw materials to gradient drying, nitrogen-protected pulverization and dry mixing, and then to nitrogen-filled sealed packaging, the oxidative degradation path of active ingredients is blocked throughout the process, forming a dual guarantee of immediate preservation and long-term stable preservation with the endogenous anchoring process.

[0096] After 3 months of accelerated storage, the total bacterial count in Examples 1-3 was <100 CFU / g, far below the national food safety standard limit, demonstrating excellent microbial control. The total bacterial count in the comparative group increased gradually with the final moisture content of the product. The core reason for this is that the dry-mixed environment of this invention (15-20℃, relative humidity ≤30%, nitrogen protection) synergistically inhibits the growth of molds, aerobic bacteria, and other microorganisms throughout the process, reducing the risk of microbial contamination and meeting the stringent food safety requirements for immunocompromised individuals after surgery and chemotherapy / radiotherapy. This invention achieves product activity stability throughout its shelf life through endogenous anchoring and end-process protection, eliminating the need for exogenous stabilizers and antioxidants. It meets clean label requirements while ensuring stable and controllable immune repair efficacy throughout the entire storage period. The products prepared by the process of this invention are superior to existing conventional processes in terms of cell wall breaking efficiency, activity retention, storage stability, food safety, and processing adaptability, thus fully supporting the scope of protection of the claims.

[0097] Example 1 was selected as the test substance. A cyclophosphamide (CTX)-induced immunodeficiency mouse model was used to simulate the immune organ damage and immunosuppression caused by clinical radiotherapy and chemotherapy. The immune repair and regulation effects of the high selenium compound powder of the present invention were systematically evaluated.

[0098] (a) Experimental materials Test substance: High-selenium composite powder prepared in Example 1; Experimental animals: SPF-grade Kunming mice (KM), 6-8 weeks old, weighing 18-22g, source and breeding conditions are the same as in the final report, and the experiment was approved by the animal ethics committee; Main reagents: cyclophosphamide (CTX), ELISA kit (IL-2, IL-6, TNF-α, IFN-γ), sheep red blood cells, complement, etc., all of analytical grade; English abbreviation:

[0099] (II) Experimental grouping and dosing regimen After 7 days of acclimatization, mice were randomly divided into 5 groups of 10 mice each according to their body weight: blank control group (Con), model control group (Mod), low-dose high-selenium compound powder group (L), medium-dose high-selenium compound powder group (M), and high-dose high-selenium compound powder group (H). The treatment methods are as follows:

[0100] Starting from the first day of the experiment, except for the control group, mice in all other groups were intraperitoneally injected with CTX to induce an immunodeficient state. The injections were administered once on days 1, 3, and 5, at a dose of 80 mg / kg. -1 The control group received three consecutive injections; the blank control group received an equal volume of physiological saline injected intraperitoneally at the same time point.

[0101] Starting from day one, the low, medium, and high dose groups of the compound powder were administered 455, 910, and 1820 mg / kg of compound powder via gavage, respectively, in addition to CTX injection. -1 ·d -1 Mice were administered 0.1 mL / 10 g body weight via gavage once daily for 15 days. The control group and model group were simultaneously administered an equal volume of physiological saline via gavage daily. Mice had free access to food and water during the experiment. Relevant immune function indicators were measured at the end of the 15-day experiment.

[0102] (III) Experimental Results 1. Effects of high-selenium compound powder on immune organ indices in immunocompromised mice To evaluate the effects of high-selenium compound powder intervention on the development of immune organs in immunocompromised mice, spleen and thymus indices were measured in each group of mice. The results of changes in immune organ indices in each group are shown below. Figure 1 and Figure 2As shown, compared with the control group, the spleen index and thymus index of mice in the model group both showed a decreasing trend, with the spleen index significantly reduced (P<0.05) and the thymus index significantly reduced (P<0.01), suggesting that cyclophosphamide treatment can lead to atrophy of immune organs and impaired immune function. Compared with the model group, the spleen index of mice after intervention with high-selenium compound powder showed varying degrees of recovery, with the high-dose group showing the most significant improvement, reaching a statistically significant level (P<0.05). Regarding the thymus index, all dose groups of high-selenium compound powder also showed a recovery trend compared with the model group, with the high-dose group showing a significant effect (P<0.05), suggesting that it may have a certain ameliorative effect on cyclophosphamide-induced immune organ damage. * indicates comparison with the control group, # indicates comparison with the model group; *P<0.05, **P<0.01; #P<0.05, ##P<0.01. In conclusion, high-selenium compound powder can improve the immune organ indices of immunocompromised mice to a certain extent.

[0103] 2. Effects of high-selenium compound powder on spleen tissue of immunocompromised mice To further evaluate the effects of high-selenium compound powder on the structure of immune organs in immunocompromised mice at the histological level, HE staining was performed on the spleen tissues of mice in each group. The results are as follows: Figure 3 As shown, the spleen tissue structure of mice in the control group was intact, with a clear boundary between the white and red pulp, intact lymphoid nodules, and dense lymphocyte distribution. In the model group, mice had more red pulp, loosely arranged lymphocytes, and reduced local tissue structural integrity. After intervention with high-selenium compound powder, the morphology of the spleen tissue in mice of all dose groups improved to varying degrees. Among them, the white pulp area in the medium and high dose groups was larger than that in the model group, the boundary between the red and white pulp was clearer, the lymphocyte distribution was denser, and the tissue structure was closer to that of the control group.

[0104] 3. Effects of high-selenium compound powder on delayed-type hypersensitivity reactions in immunocompromised mice To evaluate the effect of high-selenium compound powder on cellular immune function in immunocompromised mice, a delayed-type hypersensitivity (DTH) model was used for analysis, with paw swelling as an indicator of T lymphocyte-mediated immune response. Results are as follows: Figure 4 As shown, compared with the control group, cyclophosphamide treatment significantly reduced paw swelling in mice (P < 0.01), indicating that cellular immunity was significantly suppressed in the immunodeficiency model. Compared with the model group, the paw swelling response in mice was significantly enhanced after intervention with the high-selenium compound powder (P < 0.05). The improvement was more pronounced in the medium and high-dose groups, while the low-dose group showed relatively smaller changes. Further analysis revealed that the improvement of the DTH response by the high-selenium compound powder was mainly observed in the medium and high-dose range, suggesting that it has a certain supporting effect on T cell-mediated cellular immunity, and that this effect may have a certain dose threshold.

[0105] 4. Effects of high-selenium compound powder on humoral immune function (HC50) in immunocompromised mice To evaluate the effect of high-selenium compound powder on humoral immune function in immunocompromised mice, the serum half-hemolytic value (HC50) was used as an indicator of humoral immune response capacity. The results are as follows: Figure 5 As shown in the figure, compared with the control group, cyclophosphamide treatment significantly reduced serum HC50 levels in mice (P < 0.01), indicating that humoral immunity was suppressed in the immunodeficiency model. Compared with the model group, HC50 levels in mice at all doses of the high-selenium compound powder intervention showed varying degrees of recovery. Among them, the HC50 levels in the medium and high-dose high-selenium compound powder groups were significantly higher than those in the model group (P < 0.01), while the low-dose group showed a recovery trend, but the difference did not reach a statistically significant level. The above results indicate that under the experimental conditions, medium and high doses of high-selenium compound powder can improve humoral immunity in immunodeficient mice to a certain extent. Comprehensive analysis suggests that the regulatory effect of high-selenium compound powder on immune function in immunodeficient mice can improve humoral immunity to a certain extent under higher dose conditions, suggesting that its immune homeostasis regulation effect may have a certain dose-dependent characteristic.

[0106] 5. Effects of high-selenium compound powder on the phagocytic function of macrophages in immunocompromised mice To evaluate the effect of high-selenium compound powder on the non-specific immune function of immunocompromised mice, the carbon clearance assay was used to determine the phagocytic function of mouse macrophages. The phagocytic index (α) was used to reflect the phagocytic capacity of the mononuclear-macrophage system. The results are as follows: Figure 6 As shown in the figure, the results indicated that there were some differences in the phagocytic index of macrophages among the groups of mice, but the difference between the blank group and the model group did not reach a statistically significant level (P>0.05). Compared with the model group, there were also no statistically significant changes in the phagocytic index of macrophages in the different dose groups of the high-selenium compound powder (P>0.05). These results indicate that under the experimental conditions, cyclophosphamide treatment did not significantly affect the phagocytic function of mouse macrophages, and the intervention with the high-selenium compound powder did not significantly alter the phagocytic capacity of macrophages in immunocompromised mice. Combined with the changes in other immune function indicators in this study, it can be concluded that the regulatory effect of the high-selenium compound powder on the immune function of immunocompromised mice is not mainly reflected in the macrophage-mediated non-specific immune process.

[0107] 6. Effects of high-selenium compound powder on serum cytokine levels in immunocompromised mice To further analyze the effects of high-selenium compound powder on immune function at the level of immune regulatory networks, the levels of immune-related cytokines such as IL-2, IL-6, IFN-γ, and TNF-α in the serum of mice in each group were detected. The results are as follows: Figure 7 , Figure 8 , Figure 9 and Figure 10 As shown in the figure. Compared with the control group, the serum IL-2 and IL-6 levels in the model group were significantly decreased after cyclophosphamide treatment (P < 0.001); at the same time, IFN-γ and TNF-α levels increased, suggesting that the cytokine secretion spectrum is disordered and accompanied by immune regulation imbalance under immunodeficiency. Compared with the model group, the IL-2 level was significantly increased in the medium and high dose groups after intervention with high selenium compound powder (P < 0.01), and the IL-6 level was significantly increased in the medium and high dose groups of high selenium compound powder (P < 0.01), indicating that high selenium compound powder can promote the recovery of T cell-related cytokine secretion in immunodeficient mice to a certain extent. Regarding inflammation-related factors, compared with the model group, the TNF-α level in the high-dose group of high selenium compound powder was significantly decreased (P < 0.05), while there was no significant difference between the low and medium dose groups; in addition, the IFN-γ level in the model group was higher than that in the control group, while the high-dose group of high selenium compound powder could significantly downregulate IFN-γ (P < 0.05), suggesting that high selenium compound powder has a certain corrective effect on abnormally elevated effector factors. In summary, the high-selenium compound powder intervention can improve the cyclophosphamide-induced immunodeficiency state to a certain extent. Its immunomodulatory effect is mainly reflected in promoting the recovery of T cell-related factors such as IL-2, and it has a certain regulatory ability on abnormal levels of TNF-α, IFN-γ, etc.

[0108] Using the high-selenium compound powder prepared in Example 1 as the research object, and based on a cyclophosphamide-induced immunosuppressed mouse model, its immunomodulatory effect was systematically evaluated from the perspective of food function evaluation. The results showed that the immune organ index of the model group mice decreased, delayed-type hypersensitivity was weakened, humoral immune function was impaired, and serum IL-2 and IL-6 levels decreased while IFN-γ and TNF-α levels increased. These results suggest that under immunosuppression, T cell-related immune function is suppressed, accompanied by immune homeostasis imbalance. These changes are basically consistent with previous research results on immunosuppressed animal models, indicating that the model constructed in this study is reliable and can be used for food function evaluation research. This study focuses on the mild regulatory effect of high-selenium compound powder on immune homeostasis under dietary intake conditions from the perspective of food function evaluation, without setting a positive control to avoid direct analogy between food function and pharmacological effects. Further histological analysis was conducted on the effects of high-selenium compound powder on the immune organs of immunosuppressed mice, and HE staining was performed on the spleen tissue of mice. The results showed that the spleen tissue structure of mice in the control group was intact, with a clear boundary between the white and red pulp, intact lymphoid nodules, and dense lymphocyte distribution, indicating an overall morphology at a normal immune state level. Compared with the control group, the spleen tissue structure of mice in the model group showed certain changes, mainly manifested as an increase in red pulp and looser cell arrangement, suggesting that cyclophosphamide treatment had an inhibitory effect on spleen tissue structure. This change was consistent with the decrease in spleen index and weakened cellular immune function. After intervention with high-selenium compound powder, the spleen tissue structure of mice in each dose group showed varying degrees of improvement. Among them, the white pulp area in the medium and high dose groups was significantly restored compared with the model group, the boundary between red and white pulp became clearer, the lymphocyte distribution was more dense, and the overall morphology was close to that of the control group. The above results indicate that the regulatory effect of high-selenium compound powder on immune function is not only reflected in functional indicators, but also to a certain extent in the improvement of immune organ tissue structure. Its effect is closer to supporting and restoring immune homeostasis than strongly stimulating or pathologically repairing the immune system. At the immunological level, after intervention with the high-selenium compound powder, delayed-type hypersensitivity reactions were significantly enhanced in mice in the medium and high-dose groups, and serum IL-2 levels were significantly increased. IL-2 is an important cytokine for maintaining T cell proliferation and immune response expansion, and its recovery is generally considered an important marker of improved immune function. Therefore, the results of this study indicate that the immunomodulatory effect of the high-selenium compound powder in immunodeficiency is mainly manifested in supporting and restoring T cell-related adaptive immune function, rather than in the widespread activation of the immune system. Regarding other immune-related indicators, IL-6 was elevated in the medium and high-dose high-selenium compound powder groups. Combined with the recovery of IL-2 and IL-6 and the regulation of TNF-α and IFN-γ under high-dose conditions in this study, it can be concluded that the effect of the high-selenium compound powder on the immune network is closer to the process of restoring immune homeostasis than to unidirectional pro-inflammatory activation.Furthermore, the carbon clearance assay in this study did not show a significant effect of the high-selenium compound powder on macrophage phagocytic function, indicating that innate immune phagocytosis was not the primary target of the high-selenium compound powder under the cyclophosphamide-induced immunosuppression model and observation endpoint. Therefore, this study mainly used delayed-type hypersensitivity reaction, IL-2, and other adaptive immune-related indicators to define the immune homeostasis regulatory characteristics of the high-selenium compound powder. In summary, based on the results of immune organ indices, cellular immunity, humoral immunity, and immune-related cytokines in the cyclophosphamide-induced immunosuppression mouse model, the high-selenium compound powder exhibits a mild immune homeostasis regulatory mode that supports T cell-related adaptive immune function while also regulating immune homeostasis. The aforementioned functional improvement trend was also reflected to some extent at the spleen tissue structure level. This characteristic aligns with the functional positioning of food ingredients for immune homeostasis regulation in food functional evaluation as safe, mild, and suitable for long-term dietary intervention. Therefore, it can be concluded that the high-selenium compound powder can effectively improve the immune function of immunosuppressed mice under the conditions of this study, and its immune-enhancing effect is well-supported.

[0109] The applicant declares that the present invention is illustrated by the above embodiments, but the present invention is not limited to the above process steps, that is, it does not mean that the present invention must rely on the above process steps to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent substitutions of the raw materials used in the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

Claims

1. A method for preparing a high-selenium composite powder for postoperative and radiotherapy / chemotherapy rehabilitation and immune repair, characterized in that, include: S1. Mix high-selenium corn germ powder, selenium-enriched broccoli powder, puffed corn powder, corn powder and Chinese yam powder with the first wetting liquid, with a material-to-liquid ratio of 1:(2.5~3.5), and wet at 2~6℃ for 8~18 minutes to obtain the first wetting material; Selenium-enriched black fungus powder and selenium-enriched monkey head mushroom powder are mixed with the second wetting liquid at a ratio of 1:(1.5~2.5) and wetted at 2~6℃ for 3~12 minutes to obtain the second wetting material. The first wetting solution is a sterile deionized aqueous solution with a pH of 7.8 to 8.2; The second wetting solution is a sterile deionized aqueous solution with a pH of 6.8 to 7.2; S2. Combine the first wetting material and the second wetting material, add a compound enzyme preparation to carry out an enzymatic hydrolysis reaction, and obtain the enzymatic hydrolysate. The compound enzyme preparation includes cellulase and neutral protease; S3. Cool the enzymatically hydrolyzed material to 2-6°C and stir continuously at this temperature for 15-30 minutes to obtain the anchoring material; S4. Dry the anchoring material to a moisture content of ≤5%, and pulverize it under a protective atmosphere to a powder particle size D90 ≤20μm to obtain an enzymatically hydrolyzed cell wall breaking base material; S5. The enzymatically hydrolyzed cell wall breaking base material is dry-mixed with the thermosensitive active raw material group to obtain the high selenium composite powder; The thermosensitive active ingredient group includes at least one of epigenetic powder, corn oligopeptide powder, pea peptide powder, concentrated whey protein powder, yeast β-glucan, epigallocatechin gallate, and polydextrose.

2. The preparation method according to claim 1, characterized in that, Each raw material, by weight, includes: 20-28 parts of high-selenium corn germ powder; 4-6 parts of selenium-enriched black fungus powder; 4-6 parts of selenium-enriched broccoli powder; 4-6 parts of selenium-enriched monkey head mushroom powder; 8-12 parts puffed corn flour; 8-12 parts cornmeal; 4-6 parts of Chinese yam powder; 8-15 parts of post-natal vitamin powder; 5-10 parts corn oligopeptide powder; 5-10 parts of pea peptide powder; 4-8 parts concentrated whey protein powder; 1.5 to 3 parts of yeast β-glucan; 1-2 parts of epigallocatechin gallate; Polydextrose 2-5 parts.

3. The preparation method according to claim 1, characterized in that, In step S2, the cellulase has an enzyme activity of 50,000 to 200,000 U / g; the neutral protease has an enzyme activity of 50,000 to 200,000 U / g.

4. The preparation method according to claim 3, characterized in that, In step S2, the mass ratio of the cellulase to the neutral protease is 1:(0.8~1.2).

5. The preparation method according to claim 4, characterized in that, In step S2, the total amount of the compound enzyme preparation added is 0.6% to 1.5% of the total mass of the high-selenium corn germ powder, selenium-enriched broccoli powder, puffed corn powder, corn powder, Chinese yam powder, selenium-enriched black fungus powder and selenium-enriched monkey head mushroom powder.

6. The preparation method according to claim 5, characterized in that, In step S2, the conditions for the enzymatic hydrolysis reaction are: temperature 38~48℃, pH value 6.5~7.5, and time 45~120 minutes; The preferred temperatures are 42-45℃, pH 6.8-7.2, and time 60-90 minutes.

7. The preparation method according to claim 1, characterized in that, In step S4, the drying process specifically involves gradient drying, including: Pre-freezing: Pre-freeze at -60℃ to -50℃ for 3 to 5 hours; Vacuum freeze drying: freeze-dry at -45℃ to -35℃ and a vacuum of 10~50Pa for 10~15 hours; Low-temperature microwave drying: Under the condition that the material temperature is ≤45℃, microwave dry for 20~40 minutes until the material moisture content is ≤5%.

8. The preparation method according to claim 1, characterized in that, In step S5, the dry mixing is carried out under a protective atmosphere; The dry mixing conditions are a temperature of 15~20℃, a relative humidity of ≤30%, and a protective atmosphere of nitrogen.

9. The preparation method according to claim 1, characterized in that, In the thermosensitive active raw material group: The postbiotic powder is IOBPRO® probiotic segmented solid-state fermented postbiotic powder. The concentrated whey protein powder has a protein content of ≥70%, and osteopontin accounts for ≥90% of the total protein.

10. A high-selenium compound powder for postoperative and radiotherapy / chemotherapy rehabilitation and immune repair, characterized in that, It is prepared by any one of claims 1 to 9.

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