Dialysis and separation equipment for umbilical cord blood

Abstract

The utility model provides dialysis and separation equipment for umbilical cord blood. The dialysis and separation equipment comprises a first separation plate, a second separation plate, a bag and dredging units, wherein the first separation plate and the second separation plate are composed of filter membranes and impermeable membranes, the bag is made of flexible materials, and the dredging units are arranged on the two sides of the bag and used for squeezing. The filter membrane and the impermeable membrane are tightly combined to form an outlet, and the bag is divided into three cavities which are respectively used for treating umbilical cord blood and controlling liquid flow through a catheter and a valve. The dredging unit periodically extrudes the bag through the extrusion part and the control part, so that the filter membrane is prevented from being blocked. The equipment can effectively separate blood components through the matching of the filter membranes with different pore diameters and functions, and realizes a reliable, effective, simple and economical umbilical cord blood dialysis and separation mode.

Description

Technical Field

[0001] The utility model relates to the field of blood treatment, specifically to a hemodialysis and separation device for blood, and more specifically to a device for dialyzing and separating the components of umbilical cord blood. Background Art

[0002] Umbilical cord blood is an important part of the fetal blood circulation system in the mother's body, containing rich stem cells, including hematopoietic stem cells, mesenchymal stem cells, etc. These stem cells have important application values in clinical medicine and regenerative medicine. With the progress of science and technology, the extraction and application of umbilical cord blood stem cells have received more and more attention. Especially in the treatment of leukemia, immunodeficiency diseases, and some genetic diseases, umbilical cord blood stem cells have shown great potential. Therefore, it is particularly important to research and develop efficient umbilical cord blood dialysis and separation technologies. Currently, the dialysis and separation of umbilical cord blood are mainly for extracting stem cells from it. However, umbilical cord blood contains a large number of components such as white blood cells, red blood cells, and platelets, and these components need to be effectively separated during the process of extracting stem cells. In the prior art, osmotic membrane separation technology is usually used for the dialysis and separation of umbilical cord blood, but this technology faces some challenges in practical applications.

[0003] In the prior art, there is a method of using osmotic membrane separation technology to separate white blood cells from stem cells through a membrane with a specific pore size. However, due to the large number of white blood cells, aggregates are easily formed on the membrane surface, resulting in membrane pore blockage and reduced separation efficiency. In addition, the separation of red blood cells is also an important link in umbilical cord blood dialysis. The presence of red blood cells will interfere with the purification process of stem cells, so it needs to be separated from umbilical cord blood. Although osmotic membrane separation technology can effectively separate red blood cells, due to the huge number and easy deformation of red blood cells, they are easily accumulated on the osmotic membrane surface, further exacerbating the problem of membrane pore blockage. During the extraction process of umbilical cord blood stem cells, the presence of platelets will affect the purity of stem cells. Osmotic membrane separation technology shows certain effects in platelet separation, but due to the adhesiveness of platelets, an adhesion layer is easily formed on the membrane surface, resulting in a decline in separation effect. Thus, the main problems faced by the existing osmotic membrane separation technology in the process of umbilical cord blood dialysis and separation are that the membrane pores are blocked or blocked by aggregates, and since components such as white blood cells, red blood cells, and platelets are easily formed into aggregates or adhesion layers on the membrane surface during the separation process, these aggregates not only reduce the separation efficiency but also may cause damage to the membrane, increasing the cost and time of the separation process. In order to overcome the above-mentioned technical problems existing in the prior art, the utility model provides a dialysis and separation device for umbilical cord blood, which can solve the problem of filter membrane blockage through a simple setting method, and can flexibly set and combine multiple filter membranes to separate different components in umbilical cord blood at low cost and effectively. Summary of the Invention

[0004] The present utility model provides a device including a first separation plate, a second separation plate, a bag, and a dredging unit. Both the first separation plate and the second separation plate are composed of a rectangular filter membrane and a rectangular impermeable membrane. The length of the top of the filter membrane above is longer than that of the impermeable membrane, and the extended part of the filter membrane constitutes the outlets of the first separation plate and the second separation plate. The bag is made of a flexible material and is used to carry umbilical cord blood for dialysis and separation. The bottom ends of the first separation plate and the second separation plate are fixed to the bottom of the bag, and the filter membranes at the top ends are fixed to the top of the bag, thereby dividing the bag into a first chamber, a second chamber, and a third chamber. A supply part for storing the umbilical cord blood to be processed is communicated with the first chamber of the bag through a first conduit via a first inlet. A first outlet connected to a second pipe is provided at the bottom of the second chamber, and a second outlet connected to a third pipe is provided at the bottom of the third chamber. The dredging unit is arranged on both sides of the bag and is respectively used to squeeze the bag, the first separation plate, and the second separation plate. The bottom edges of the filter membrane and the impermeable membrane, the left edges of the filter membrane and the impermeable membrane, and the right edges of the filter membrane and the impermeable membrane are tightly combined together to form connection lines, and no connection lines are provided above the filter membrane and the impermeable membrane. The bottom ends of the first separation plate and the second separation plate are fixed to the bottom of the bag through the connection lines. The flow of the liquid inside the first conduit is controlled by a first valve, the flow of the liquid inside the second pipe is controlled by a second valve, and the flow of the liquid inside the third pipe is controlled by a third valve. The dredging unit is respectively provided with squeezing parts for squeezing the bag on both sides of the bag. The dredging unit also includes a control part for controlling the squeezing displacement of the squeezing parts and an actuator for sending a squeezing command signal to the control part.

[0005] With the above arrangement, the present utility model is provided with multiple separation plates in the bag, enabling the blood components to undergo dialysis in a sequential gradient manner. By matching filter membranes with different pore sizes and functions, specific cells can be separated in the chambers between the separation plates. Additionally, in cooperation with the periodic squeezing of both sides of the bag by the dredging unit, the blood can be made to flow and the positions of the filter membrane surfaces can be moved, effectively preventing the blood components from clogging the filter membrane. Thus, the present utility model realizes the dialysis and separation of umbilical cord blood in a reliable, effective, simple, and economical manner. BRIEF DESCRIPTION OF THE DRAWINGS

[0006] Figure 1 is a perspective structural schematic diagram of the device of the present utility model;

[0007] Figure 2 is a front and side schematic diagram of the separation plate in the device of the present utility model. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0008] The following will detail the embodiments of the present utility model in conjunction with embodiments, enabling a full understanding of the implementation process of how to apply technical means to solve technical problems and achieve technical effects for implementation.

[0009] The device 30 of the present utility model is used for dialysis and separation of the components of umbilical cord blood, as shown in the appended Figure 1-2 figure. In the device 30, there are a first separation plate 1 and a second separation plate 101 for dialysis and separation of umbilical cord blood. The structures of the first separation plate 1 and the second separation plate 101 are the same. The above separation plate includes a filter membrane 2 and an impermeable membrane 3. The filter membrane 2 is rectangular and made of a semi-permeable material. The filter membrane 2 has a first thickness 6. The filter membrane 2 is provided with a first front surface 4 and a first rear surface 5. The central axis along the length direction of the filter membrane 2 is A. An impermeable membrane 3 is provided in the direction of the first rear surface 5 of the filter membrane 2. The impermeable membrane 3 is also rectangular and is arranged in a parallel manner to the filter membrane 2. The impermeable membrane 3 also has a second thickness 13. The impermeable membrane 3 is provided with a second front surface 11 and a second rear surface 12. The central axis along the length direction of the impermeable membrane 2 is B. The bottom edges of the filter membrane 2 and the impermeable membrane 3, the left side edges of the filter membrane 2 and the impermeable membrane 3, and the right side edges of the filter membrane 2 and the impermeable membrane 3 are tightly combined together, thus forming a connecting line 18. No connecting line 18 is provided above the filter membrane 2 and the impermeable membrane 3. The filter membrane 2 and the impermeable membrane 3 are combined to form a separation plate. Above, the impermeable membrane 3 is at a first height 14. The length of the filter membrane 2 is longer than the first height 14. The part by which the filter membrane 2 extends beyond the impermeable membrane 3 constitutes the opening 19 of the separation plate.

[0010] The device 30 of the present utility model is also provided with a bag 21 made of a flexible material for containing umbilical cord blood, and a first separation plate 1 and a second separation plate 101 located inside the bag 21. The first separation plate 1 and the second separation plate 101 are both fixed to the bottom of the bag 21 through the connecting line 18. The filter membrane 2 at the top of the first separation plate 1 and the second separation plate 101 is fixed to the top of the bag 21. Thus, the first separation plate 1 and the second separation plate 101 divide the bag 21 into three chambers. There are two separation plates in the bag 21. Thus, the bag 21 is divided into a first chamber 22, a second chamber 122, and a third chamber 23 from left to right. The supply part 31 located at the top of the device 30 is used for storing the umbilical cord blood to be processed. The supply part 31 passes through a first catheter 32 and is controlled by a first valve 33 to enter the first chamber 22 of the bag 21 through a first inlet 24. The umbilical cord blood is dialyzed and filtered through the filter membrane 2 of the first separation plate 1 in the first chamber 22 to its top outlet 19 and reaches the second chamber 122. The bottom of the second chamber 122 is provided with a first outlet 25 connected to a second pipe 34. A second valve 35 is provided on the second pipe 34 to control the outflow of the liquid. The liquid inside the second chamber 122 is dialyzed and filtered through the filter membrane 2 of the second separation plate 101 to its top outlet 19 and reaches the third chamber 23. The bottom of the third chamber 23 is provided with a second outlet 27 connected to a third pipe 39. A third valve 40 is provided on the third pipe 39 to control the outflow of the liquid in the third chamber 23.

[0011] The device 30 of the present utility model is further provided with a dredging unit 41. The dredging unit 41 is respectively provided with extrusion parts 42 for extruding the bag 21 on both sides of the bag 21, a control part 43 for controlling the extrusion displacement of the extrusion parts 42, and an actuator 44 for sending an extrusion command signal to the control part 43. Through the cooperation of the above components, a periodic extrusion action on the inside of the bag 21 can be realized, so that the blood fluid inside the bag 21 generates a reciprocating flow, thereby alleviating the blockage of the blood components to the filter membrane 2. Through the above setting method of the present utility model, a plurality of separation plates are provided in the bag 21 so that the blood components can be dialyzed in sequence in a gradient manner. By matching filter membranes with different pore sizes and functions, specific cells can be separated in the chambers between the separation plates. In addition, by cooperating with the periodic extrusion of the dredging unit 41 on both sides of the bag 21, the blood can be made to flow and the position of the surface of the filter membrane 2 can be moved, thereby effectively preventing the blood components from blocking the filter membrane. Thus, the present utility model realizes dialysis and separation of umbilical cord blood in a reliable, effective, simple and economical manner.

[0012] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A dialysis and separation device for umbilical cord blood, characterized in that: The device (30) comprises a first separation plate (1), a second separation plate (101), a bag (21) and a dredging unit (41); the first separation plate (1) and the second separation plate (101) are both composed of a rectangular filter membrane (2) and a rectangular impermeable membrane (3); the length of the top of the filter membrane (2) is longer than the impermeable membrane (3); the extended portion of the filter membrane (2) constitutes the outlet (19) of the first separation plate (1) and the second separation plate (101); the bag (21) is made of a flexible material and is used for carrying umbilical cord blood and performing dialysis and separation; the bottom ends of the first separation plate (1) and the second separation plate (101) are fixed to the bottom of the bag (21), and the filter membrane (2) at the top of the first separation plate (1) and the second separation plate (101) is fixed to the top of the bag (21), thereby dividing the bag (21) into a first chamber (22), a second chamber (122), and a third chamber (23); The supply unit (31) for storing umbilical cord blood to be processed is connected to the first chamber (22) of the bag (21) via a first conduit (32) and a first inlet (24); the bottom of the second chamber (122) is provided with a first outlet (25) connected to a second pipe (34); the bottom of the third chamber (23) is provided with a second outlet (27) connected to a third pipe (39); the dredging unit (41) is provided on both sides of the bag (21) and is used to squeeze the bag (21), the first separation plate (1) and the second separation plate (101) respectively.

2. The device according to claim 1, characterized in that The bottom edge of the filter membrane (2) and the bottom edge of the impermeable membrane (3), the left side of the filter membrane (2) and the left side of the impermeable membrane (3), and the right side of the filter membrane (2) and the right side of the impermeable membrane (3) are tightly combined to form a connecting line (18), and the connecting line (18) is not arranged above the filter membrane (2) and the impermeable membrane (3); the bottom ends of the first separation plate (1) and the second separation plate (101) are fixed to the bottom of the bag (21) through the connecting line (18).

3. The device according to claim 1, characterized in that The first conduit (32) is controlled by a first valve (33) for controlling the flow of liquid therein, the second conduit (34) is controlled by a second valve (35) for controlling the flow of liquid therein, and the third conduit (39) is controlled by a third valve (40) for controlling the flow of liquid therein.

4. The device according to claim 1, characterized in that The dredging unit (41) is provided with squeezing parts (42) for squeezing the bag (21) on both sides of the bag (21), and the dredging unit (41) is also provided with a control part (43) for controlling the squeezing displacement of the squeezing part (42) and an actuator (44) for sending a squeezing command signal to the control part (43).

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