Special glass slide for stimulated Raman scattering imaging microscope

Abstract

The utility model discloses a special glass slide for a stimulated Raman scattering imaging microscope, which comprises a glass slide, a cover glass and a buckle, the glass slide is in limit connection with the cover glass through the buckle, a sample groove is arranged on the glass slide, the cover glass covers the sample groove, so that a cavity is formed between the glass slide and the cover glass, and the sample groove is arranged in the cavity. And the side surface of the glass slide is provided with at least two small holes which are directly communicated with the inner cavity of the sample groove. According to the utility model, water evaporation can be prevented, bubbles can be avoided, water can be replenished without uncovering the cover glass, and laser is ensured to be focused on the same plane of a sample during imaging.

Description

Technical Field

[0001] The utility model relates to a special glass slide for stimulated Raman scattering imaging microscope and belongs to the technical field of medical equipment parts. Background Art

[0002] The increasingly in-depth scientific research system has put forward higher requirements for the specific imaging of different biochemical components of samples. People are no longer satisfied with the contrast obtained by ordinary white light microscopes relying on light absorption, and have developed a series of microscopy techniques such as phase contrast, differential interference, and fluorescence. Stimulated Raman scattering imaging microscopy stands out from many microscopy techniques with its advantages of label-free and fast imaging. However, as a method of laser imaging, in order to avoid light damage to the sample during the imaging process, researchers need to ensure that there is enough water around the sample.

[0003] However, the common slides used in the laboratory have the following problems: ① the water is easy to evaporate; ② the tape is easy to fall off and the adhesion is not tight when sticking the cover glass; ③ bubbles are easy to form around the tissue when sticking the cover glass, which affects the imaging quality. Therefore, the development of a special slide for stimulated Raman scattering imaging microscope is expected to solve the above problems.

[0004] The current slides have the following specific problems:

[0005] 1. Most of the existing inventions are various types of perforated glass slides, but a glass slide needs to be glued to the bottom of the perforated glass slide by adhesive to carry the sample, which is cumbersome and lacks practicality;

[0006] 2. When using a cover glass to flatten the tissue, it is necessary to seal the coverslip with tape at both ends, and there is no snap-on design;

[0007] 3. During long-term imaging, the sample is burned due to the evaporation of water from around the cover glass. The cover glass needs to be frequently opened for water replenishment, which is inconvenient to operate and affects the continuity of the experiment.

[0008] 4. Ordinary glass slides may have some tiny rough surfaces, which will cause the incident light to be scattered / reflected before it contacts the sample, thus reducing the intensity of the received signal. Summary of the invention

[0009] The technical problems to be solved by the utility model are: how to avoid the problem of easy evaporation of water in the sample placed on the slide, how to enable the cover glass to be quickly fixed to the slide, and how to reduce the scattering / reflection of incident light by the cover glass and the slide.

[0010] To solve the above technical problems, the technical solution of the present utility model is to provide a special glass slide for stimulated Raman scattering imaging microscope, which is characterized by comprising a glass slide, a cover glass and a buckle. The glass slide and the cover glass are connected by the buckle in a limiting manner. A sample groove is provided on the glass slide, and the cover glass covers the sample groove, so that a cavity is formed between the glass slide and the cover glass. At least two small holes directly communicating with the inner cavity of the sample groove are provided on the side of the glass slide.

[0011] Preferably, the cover glass is arranged in the sample groove and is located at the upper opening position of the sample groove.

[0012] Preferably, the sample groove is a double-layer groove structure, including an upper groove and a lower groove which are communicated with each other. The lower groove is a circular or rectangular strip structure, and the upper groove is adapted to the cover glass.

[0013] Preferably, the cross-sectional area of the upper groove is larger than that of the lower groove.

[0014] Preferably, at least one mounting hole is provided on each side of the sample groove on the glass slide; the tail of the buckle is inserted into the mounting hole, and the lower surface of the head of the buckle abuts against the cover glass.

[0015] Preferably, for the small holes on both sides of the glass slide, at least one small hole is connected to a syringe, and the other small hole is connected to the atmosphere or a gas pumping and ventilation device (which can be an inflation air pump and a vacuum pump) for balancing the air pressure.

[0016] Preferably, both the glass slide and the cover glass are transparent structures.

[0017] Preferably, an anti-reflection coating is provided on both the glass slide and the cover glass.

[0018] Compared with the prior art, the present utility model has at least one of the following beneficial effects:

[0019] ① The nearly fully enclosed structure is beneficial to preventing water evaporation;

[0020] ② By filling the sample groove in the form of injecting PBS or physiological saline, the generation of air bubbles can be avoided;

[0021] ③ When additional water replenishment is required, it is not necessary to uncover the cover glass, avoiding damaging the initial form of the sample;

[0022] ④ By pressing the cover glass in the form of a buckle, the operation is simple, ensuring that the laser is focused on the same plane of the sample during imaging, and the plastic buckle can be reused.

[0023] ⑤ By applying an anti-reflection coating on the front surfaces of the glass slide and the cover glass, the scattering / reflection problems are reduced in two stages before and after the incident light irradiates the sample, improving the intensity of the received signal and the imaging quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is a front view (circular groove) of a special slide for a stimulated Raman scattering imaging microscope;

[0025] Figure 2 is a side view (circular groove) of a special slide for a stimulated Raman scattering imaging microscope;

[0026] Figure 3 is a top view (circular groove) of a special slide for a stimulated Raman scattering imaging microscope;

[0027] Figure 4 is a front view (long strip groove) of a special slide for a stimulated Raman scattering imaging microscope;

[0028] Figure 5 is a side view (long strip groove) of a special slide for a stimulated Raman scattering imaging microscope;

[0029] Figure 6 is a top view (long strip groove) of a special slide for a stimulated Raman scattering imaging microscope. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0030] For the present utility model to be more clearly understood, the preferred embodiments will be described in detail below in conjunction with the accompanying drawings.

[0031] The present utility model provides a special slide for a stimulated Raman scattering imaging microscope, which is used to carry various tissues and materials in Raman scattering imaging, such as Figures 1-6 shown, which is composed of a slide 1, a cover glass 2 and a buckle 3. The slide 1 and the cover glass 2 are made of quartz materials with high heat resistance, high purity and transparency, and an anti-reflection coating SiO2 (with a thickness of 600 nm) is applied on the surface to ensure that it will not deform or affect the imaging quality under laser irradiation.

[0032] A sample groove 6 is provided in the middle of the slide 1. The sample groove 6 is a double-layer groove structure, including an upper groove 61 and a lower groove 62. The lower groove 62 is a circular or long strip structure (such as Figure 3 , Figure 6 shown), with a depth of 500 μm and a moderate area, and sufficient water storage space (cavity) should be reserved. The upper groove 61 is adapted to the cover glass 2, so that the slide 1 and the cover glass 2 form a fully enclosed structure to prevent water evaporation.

[0033] Two small holes 5 are designed on each side of the glass slide 1, which are directly connected to the inner cavity of the sample cell 6. A syringe can be used to directly inject water into one of the small holes 5 on each side, and the other small hole 5 on each side is used to balance the air pressure. The diameter of the small hole 5 is about 400μm, so that a 28G or finer needle can pass through the small hole 5 for water injection.

[0034] On the glass slide 1, an installation hole 4 is drilled on each side of the sample cell 6 for installing a circular plastic buckle 3. The tail of the buckle 3 is inserted into the installation hole 4, and the lower surface of the head of the buckle 3 abuts against the cover glass 2, so as to limit the cover glass 2 in the sample cell 6 of the glass slide 1.

Claims

1. A special slide for stimulated Raman scattering imaging microscope, characterized in that: The invention comprises a glass slide (1), a cover glass (2) and a buckle (3); the glass slide (1) and the cover glass (2) are connected in a limited position by the buckle (3); a sample groove (6) is provided on the glass slide (1); the cover glass (2) covers the sample groove (6) so that a cavity is formed between the glass slide (1) and the cover glass (2); and at least two small holes (5) are provided on the side of the glass slide (1) and are directly connected to the cavity in the sample groove (6).

2. A special slide for stimulated Raman scattering imaging microscope according to claim 1, characterized in that: The cover glass (2) is arranged in the sample groove (6) and is located at the upper opening of the sample groove (6).

3. A special slide for stimulated Raman scattering imaging microscope as claimed in claim 1, characterized in that: The sample groove (6) is a double-layer groove structure, comprising an upper groove (61) and a lower groove (62) that are interconnected, the lower groove (62) is a circular or elongated structure, and the upper groove (61) is adapted to the cover glass (2).

4. A special slide for stimulated Raman scattering imaging microscope as claimed in claim 3, characterized in that: The cross-sectional area of ​​the upper groove (61) is greater than the cross-sectional area of ​​the lower groove (62).

5. A special slide for stimulated Raman scattering imaging microscope according to claim 1, characterized in that: The slide glass (1) is provided with at least one mounting hole (4) on each of the left and right sides of the sample slot (6); the rear end of the buckle (3) is inserted into the mounting hole (4), and the lower surface of the head of the buckle (3) abuts against the cover glass (2).

6. A special glass slide for stimulated Raman scattering imaging microscope according to claim 1, characterized in that: At least one of the small holes (5) on both sides of the glass slide (1) is connected to a syringe, and the other small hole (5) is connected to the atmosphere or to an exhaust ventilation device for balancing the air pressure.

7. A special slide for stimulated Raman scattering imaging microscope according to claim 1, characterized in that: The glass slide (1) and cover glass (2) are both transparent structures.

8. A special glass slide for stimulated Raman scattering imaging microscope according to claim 1 or 7, characterized in that: The slide glass (1) and the cover glass (2) are both provided with an anti-reflection coating.

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