Paper-based DNA (deoxyribonucleic acid) rapid crude extraction kit

Abstract

The utility model relates to a DNA (deoxyribonucleic acid) rapid crude extraction kit based on a paper base, and belongs to the technical field of DNA extraction devices of paper bases. Comprising a cover plate and a bottom groove; the cover plate is detachably connected with the bottom groove; a plurality of groups of circular hollows and a plurality of groups of circular grooves are formed in the cover plate; the circular hollowed-out parts and the circular grooves are opposite in position and consistent in number on the cover plate; a rectangular groove is formed in the bottom groove; a water-absorbing paper base is mounted in the rectangular groove; and a circular DNA recovery paper substrate is arranged in the circular hollow part and is positioned at the upper part of the water-absorbing paper base. According to the kit, the cover plate and the bottom groove are detachably connected, so that the kit is convenient to carry and store; the kit adopts the design of circular hollows and circular grooves, so that the DNA recovery paper substrate is convenient to place and position, complex operation is not needed, and the DNA extraction efficiency is improved; the water-absorbing paper base is adopted to assist liquid transfer, the efficiency is high, DNA does not need to be repeatedly transferred to recover the paper base, and the paper base is effectively prevented from being broken or deformed.

Description

Technical Field

[0001] The utility model relates to the technical field of paper-based DNA extraction devices, in particular to a paper-based rapid crude DNA extraction kit. Background Art

[0002] As an emerging technology, paper-based extraction is well-suited for applications outside the laboratory due to its simplicity, speed, and low cost, especially in resource-limited settings such as farms, fields, and remote areas. Paper-based DNA extraction uses a purification mechanism that differs from other extraction systems. It is believed to exploit the differences in capture and surface chemical retention kinetics between DNA and other compounds through the pore structure of the paper substrate. DNA is separated through selective adsorption and elution. Large DNA molecules are rapidly captured by cellulose and retained during the washing step, while other smaller compounds, including some DNA amplification inhibitors, are released into the washing solution.

[0003] Existing paper-based DNA extraction methods mainly complete the adsorption, washing or elution steps by moving the paper base in different liquids. Although this method has gotten rid of the dependence on special instruments such as pipettes and centrifuges, due to differences in operating habits, the paper base is easily squeezed, broken or deformed during the transfer process, resulting in uneven force and affecting the stability of the extraction effect (unstable concentration, impurities introduced, etc.). Utility Model Content

[0004] The utility model provides a paper-based rapid crude DNA extraction kit, which adopts the following technical solution: a paper-based rapid crude DNA extraction kit, comprising:

[0005] A cover plate and a bottom trough; the cover plate and the bottom trough are detachably connected;

[0006] The cover plate is provided with a plurality of groups of circular hollows and a plurality of groups of circular grooves;

[0007] The circular hollows and the circular grooves are positioned opposite to each other and have the same number on the cover plate;

[0008] A rectangular groove is provided inside the bottom trough;

[0009] An absorbent paper base is installed in the rectangular groove;

[0010] A circular DNA recovery paper substrate is installed in the circular hollow and is located on the upper part of the absorbent paper substrate.

[0011] Furthermore, the diameter of the circular hollow is larger than the diameter of the circular groove; and the diameter of the circular DNA recovery paper substrate is smaller than the diameter of the circular groove.

[0012] Furthermore, the circular DNA recovery paper substrate is a glass microfiber filter paper disc.

[0013] Furthermore, the absorbent paper base is cotton pulp filter paper.

[0014] Furthermore, the cover plate and the bottom groove are detachably connected via a snap assembly; the snap assembly includes a plurality of mother snaps mounted on the cover plate and a plurality of child snaps mounted on the bottom groove; the mother snaps and the child snaps are positioned relative to each other and are the same in number.

[0015] Furthermore, a plurality of partitions are arranged at equal intervals in the rectangular groove to divide the rectangular groove into a plurality of grooves; and an absorbent paper base having the same size as the bottom area is built into the groove.

[0016] Furthermore, the grooves, the circular hollows and the circular grooves are positioned relative to each other and have the same number.

[0017] In summary, the present invention has the following beneficial technical effects:

[0018] This utility model can extract multiple independent or parallel samples simultaneously, with the entire process taking only approximately 10 minutes. The use of absorbent paper to assist in liquid transfer increases efficiency and eliminates the need for repeated transfer of the DNA recovery paper, effectively preventing breakage or deformation. DNA extracted using this kit can be directly used in isothermal amplification techniques such as recombinase polymerase amplification (RPA), recombinase-mediated amplification (RAA), and multienzyme isothermal rapid amplification (MIRA), further shortening the overall testing time and enabling truly rapid on-site testing.

[0019] 2. The cover and bottom tank of the test kit of the utility model are designed to be detachably connected, which is convenient for carrying and storage.

[0020] 3. The utility model adopts the design of circular hollowing and circular grooves, which facilitates the placement and positioning of the DNA recovery paper substrate, eliminates the need for complicated operations, and improves the efficiency of DNA extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic exploded view of the structure of the present utility model;

[0022] Figure 2 It is a schematic exploded view of the structure of another embodiment of the present invention.

[0023] Explanation of the accompanying symbols: 1. Cover plate; 2. Bottom groove; 3. Circular hollow; 4. Circular groove; 5. Rectangular groove; 6. Absorbent paper base; 7. Circular DNA recovery paper base; 8. Mother buckle; 9. Child buckle; 10. Partition; 11. Groove. DETAILED DESCRIPTION

[0024] The present invention will be described in further detail below with reference to the accompanying drawings.

[0025] like Figure 1 A specific embodiment of a paper-based rapid crude DNA extraction kit is shown;

[0026] A paper-based rapid crude DNA extraction kit is used to detect different parallel samples of the same batch of samples, comprising: a cover plate 1 and a bottom trough 2; the cover plate 1 and the bottom trough 2 are detachably connected; the cover plate 1 is provided with a plurality of groups of circular hollows 3 and a plurality of groups of circular grooves 4; the circular hollows 3 and the circular grooves 4 are positioned opposite to each other on the cover plate 1 and are the same in number; a rectangular groove 5 is provided inside the bottom trough 2; an absorbent paper base 6 is installed in the rectangular groove 5; a circular DNA recovery paper substrate 7 is installed in the circular hollow 3 and is located on the upper part of the absorbent paper base 6; the diameter of the circular hollow 3 is larger than the diameter of the circular groove 4; the diameter of the circular DNA recovery paper substrate 7 is smaller than the diameter of the circular groove 4; the circular DNA recovery paper substrate 7 is a glass microfiber filter paper disc; and the absorbent paper base 6 is cotton pulp filter paper.

[0027] Specifically, in this embodiment, the cover plate 1 is 112.75 mm long and 34 mm wide. There are 5 circular hollows 3 with a diameter of 10 mm on the top, and the edges extend downward in a cylindrical shape, which are the placement area of ​​the circular DNA recovery paper substrate 7, with an interval of 8 mm, for placing the circular DNA recovery paper substrate 7; there are 5 circular grooves 4 with a diameter of 8 mm and a depth of 3 mm, with an interval of 8 mm, at the bottom, which are the air-drying area of ​​the circular DNA recovery paper substrate 7.

[0028] The circular DNA recovery paper substrate 7 is a glass microfiber filter paper disc with a diameter of 6 mm, which is used to carry the crude DNA mixture.

[0029] Specifically, the air-drying area of ​​the circular DNA recovery paper substrate 7 is used to air-dry the circular DNA recovery paper substrate 7 .

[0030] The absorbent paper base 6 is a cotton pulp filter paper with a thickness of 1 mm.

[0031] Specifically, the absorbent paper substrate 6 is used to assist in liquid transfer, which is highly efficient and does not require repeated transfer of the circular DNA recovery paper substrate 7, thereby effectively preventing the paper substrate from being broken or deformed.

[0032] Specifically, in this embodiment, the detachable connection between the cover plate 1 and the bottom groove 2 is preferably detachably connected through a snap assembly; the snap assembly includes several mother snaps 8 installed on the cover plate and several child snaps 9 installed on the bottom groove; the mother snaps 8 and the child snaps 9 are positioned opposite to each other and are the same in number.

[0033] Specifically, the snap assembly makes the connection and disassembly of the cover 1 and the bottom groove 2 more convenient, without the use of tools, and is simple to operate. It can firmly connect the cover 1 and the bottom groove 2, ensuring that the reagent kit will not loosen or fall off during use, maintaining the stability of the structure; it can effectively prevent liquid leakage, avoid cross contamination, and ensure the reliability of experimental results; the snap structure can usually be reused, reducing experimental costs and improving economic benefits.

[0034] In other preferred embodiments:

[0035] Used to test different batches of samples, such as Figure 2 As shown, a number of partitions 10 are equidistantly arranged in the rectangular groove 5 to divide the rectangular groove 5 into a plurality of grooves 11; the grooves 11 contain absorbent paper bases 6 of the same size as the bottom area; the grooves 11 are opposite to the circular hollows 3 and the circular grooves 4, and the number is the same.

[0036] Specifically, in this embodiment, the cover plate 1 is 178 mm long and 34 mm wide, with 8 circular hollows 3 with a diameter of 10 mm on the top, and the edges extend downward in a cylindrical shape with a depth of 3 mm, which is the placement area of ​​the circular DNA recovery paper substrate 7, with an interval of 8 mm; there are 8 circular grooves 4 with a diameter of 8 mm and a depth of 3 mm at the bottom, with an interval of 8 mm, which is the air-drying area of ​​the circular DNA recovery paper substrate 7.

[0037] The bottom groove 2 is composed of 8 conjoined grooves 11, each groove 11 has an inner wall length of 30 mm, a width of 20 mm, and a groove depth of 5 mm. Each groove 11 has an absorbent paper base 6 of the same size as the bottom area.

[0038] The circular DNA recovery paper substrate 7 is a glass microfiber filter paper disc with a diameter of 6 mm.

[0039] The absorbent paper base is 1 mm thick cotton pulp filter paper.

[0040] Specifically, 8 independent samples or parallel samples can be extracted at one time, and the whole process only takes about 10 minutes.

[0041] Specifically, the DNA extracted using this kit can be directly used for constant-temperature amplification technologies such as recombinase polymerase amplification (RPA), recombinase-aid amplification (RAA), and multienzyme isothermal rapid amplification (MIRA), further shortening the entire detection process and achieving true on-site rapid detection.

[0042] The above are all preferred embodiments of the present invention, and are not intended to limit the scope of protection of the present invention. Therefore, any equivalent changes made based on the structure, shape, and principle of the present invention should be included in the scope of protection of the present invention.

Claims

1. A paper-based rapid crude DNA extraction kit, characterized in that: include: A cover plate and a bottom trough; the cover plate and the bottom trough are detachably connected; The cover plate is provided with a plurality of groups of circular hollows and a plurality of groups of circular grooves; The circular hollows and the circular grooves are positioned opposite to each other and have the same number on the cover plate; A rectangular groove is provided inside the bottom trough; An absorbent paper base is installed in the rectangular groove; A circular DNA recovery paper substrate is installed in the circular hollow and is located on the upper part of the absorbent paper substrate.

2. A paper-based rapid crude DNA extraction kit according to claim 1, characterized in that: The diameter of the circular hollow is larger than the diameter of the circular groove; the diameter of the circular DNA recovery paper substrate is smaller than the diameter of the circular groove.

3. A paper-based rapid crude DNA extraction kit according to claim 1, characterized in that: The circular DNA recovery paper substrate is a glass microfiber filter paper disc.

4. A paper-based rapid crude DNA extraction kit according to claim 1, characterized in that: The absorbent paper base is cotton pulp filter paper.

5. A paper-based rapid crude DNA extraction kit according to claim 1, characterized in that: The cover plate and the bottom slot are detachably connected via a snap assembly; the snap assembly includes a plurality of mother snaps mounted on the cover plate and a plurality of child snaps mounted on the bottom slot; the mother snaps and the child snaps are positioned relative to each other and are the same in number.

6. A paper-based rapid crude DNA extraction kit according to claim 1, characterized in that: A plurality of partitions are arranged at equal intervals in the rectangular groove to divide the rectangular groove into a plurality of grooves; and a water-absorbing paper base having the same size as the bottom area is built into the groove.

7. A paper-based rapid crude DNA extraction kit according to claim 6, characterized in that: The grooves, the circular hollows and the circular grooves are positioned opposite to each other and have the same number.

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