Plant virus friction receiving device

Abstract

The utility model relates to the technical field of plant virus inoculation devices, in particular to a plant virus friction virus receiving device which comprises a liquid taking box and a friction virus receiving assembly. The liquid taking box is detachably connected with the friction poison receiving assembly; the friction poison receiving assembly comprises a bottom supporting box, the bottom supporting box is movably connected with a poison receiving box, the bottom supporting box is detachably connected with a liquid taking box, and the liquid taking box and the poison receiving box are oppositely arranged; a plurality of bristles are arranged on the inner wall of the poison receiving box, and the length of the bristles is equal to the depth of the liquid taking box; a movable bottom plate is arranged in the poison receiving box, the movable bottom plate can move towards or away from the liquid taking box, and the bristles movably penetrate through the movable bottom plate. According to the friction virus receiving device provided by the utility model, the friction stress of the blades can be strictly controlled, and the stress of the whole blades is uniform, so that the condition of insufficient friction or excessive force during inoculation is avoided. The device is suitable for laboratory novice, and can reduce the number of times of experiment groping and failure.

Description

Technical Field

[0001] This utility model relates to the technical field of plant virus inoculation devices, specifically to a plant virus friction inoculation device. Background Technology

[0002] Plant viruses cause significant losses to food and cash crops worldwide. In my country, the incidence rates of diseases affecting food crops such as rice stripe virus, rice black-streaked dwarf virus, southern rice black-streaked dwarf virus, and wheat yellow dwarf virus, as well as those affecting vegetables and fruits such as tomato spotted wilt virus, cucumber mosaic virus, and melon yellow spot virus, can reach over 50%. Viruses produce symptoms such as mosaic, mottling, leaf curling, yellowing, and stunting on plants, limiting normal photosynthesis and other physiological activities, severely impacting the quality and characteristics of crops and vegetables. Therefore, strengthening research on plant viruses and preventing and reducing the harm caused by plant viral diseases is of great significance for ensuring bumper harvests and promoting national economic development.

[0003] In basic research on viral diseases, from studying symptoms and transmission vectors to understanding plant resistance mechanisms and screening for resistant varieties, almost every step requires artificial inoculation for subsequent scientific experiments. Existing artificial inoculation methods mainly include insect-borne transmission, infectious clonal transmission, and mechanical inoculation. Insect-borne transmission has advantages in preserving the virus source, but not all viruses can be transmitted by insects. Insect-borne transmission requires insect rearing and suffers from unstable transmission efficiency, uneven transmission range, and secondary damage to plant leaves, limiting its practical application. Infectious clonal transmission is more efficient and has less impact on leaves, but constructing infectious clones is time-consuming, difficult, and requires highly skilled personnel. Mechanical inoculation is the most commonly used method, and using inoculation devices can improve efficiency.

[0004] Current inoculation devices include friction inoculation devices and inoculation devices using inoculation heads. Among these, inoculation devices using inoculation heads have a limited application range due to their fixed brush head length. Friction inoculation devices most commonly use a brush, where the user creates a wound on the plant by rubbing it with the brush. While simple to operate, these devices have the following drawbacks: uneven user pressure can lead to either excessive pressure causing plant death or insufficient pressure resulting in inoculation failure. In other words, existing friction inoculation devices suffer from difficulty in controlling the friction pressure, leading to inoculation failure. Therefore, there is an urgent need to develop a new plant virus friction inoculation device to solve these problems. Utility Model Content

[0005] The purpose of this invention is to provide a plant virus friction grafting device to solve the problem that existing friction grafting devices are difficult to control in actual operation, which leads to the failure of plant grafting.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] This invention provides a plant virus friction inoculation device, including a liquid collection box and a friction inoculation component; the liquid collection box and the friction inoculation component are detachably connected.

[0008] The friction-contact assembly includes a base box, a contaminant box movably connected to the base box, and a liquid collection box detachably connected to the base box, with the liquid collection box and the contaminant box positioned opposite each other.

[0009] The inner wall of the receiving box is provided with a plurality of bristles, the length of which is equal to the depth of the liquid collection box. The receiving box has a movable base plate that can move toward or away from the liquid collection box, and the bristles move through the movable base plate.

[0010] A connecting pipe is connected to the movable base plate, and the connecting pipe passes through the poison receiving box.

[0011] This invention features a movable base plate that moves within the inoculation box, allowing adjustment of the bristle length on the side of the base plate furthest from the box. This enables users to easily control the friction intensity during operation, ensuring successful plant inoculation. The friction inoculation device provided by this invention can precisely control the friction force on the leaves, ensuring uniform force across the entire leaf and preventing insufficient or excessive friction during inoculation. It is suitable for laboratory novices, reducing the number of trial and error attempts and failures.

[0012] Preferably, a grooved cylindrical connecting block is connected to the inner wall of the receiving box, and the cylindrical connecting block and the connecting tube together form a movable piston structure.

[0013] Preferably, the movable base plate is provided with a pipe hole, and one end of the connecting pipe away from the inner wall of the receiving box passes through the pipe hole and is located on the side of the movable base plate away from the receiving box.

[0014] Preferably, the movable base plate is further provided with a pair of symmetrically arranged cylindrical holes, the distance between the two cylindrical holes is greater than the diameter of the pipe hole, and the two cylindrical holes are located on both sides of the pipe hole.

[0015] Preferably, the end of the connecting pipe away from the inner wall of the receiving box passes through the pipe hole and is detachably connected to the cylindrical connecting block.

[0016] The open end of the cylindrical connecting block passes through the cylindrical perforation and is detachably connected to the cylindrical perforation.

[0017] Preferably, the cylindrical connecting block is provided with a connecting groove; the end of the connecting tube away from the inner wall of the receiving box is provided with a connecting protrusion; the connecting protrusion is used to engage with the connecting groove.

[0018] Preferably, the connecting tube is a graduated tube.

[0019] Preferably, one end of the graduated tube passes through the inoculation box, and the other end passes through the movable base plate. By manipulating the sliding of the graduated tube inside the cylindrical connecting block, the movable base plate is moved relative to the inoculation box, thereby adjusting the effective length of the bristles. The graduated tube is a telescopic tube that can extend and retract vertically, enabling the movable base plate to move within the inoculation box, thereby adjusting the length of the bristles on the side of the movable base plate away from the inoculation box. In other words, the length of the bristles used for plant friction inoculation can be adjusted and controlled.

[0020] Preferably, the scale tube includes a disc and a connecting tube, one end of the connecting tube is connected to the disc, and the other end of the connecting tube passes through the wall of the receiving box and the pipe hole in sequence, and is engaged in the connecting groove by the connecting protrusion.

[0021] Preferably, the movable base plate is further provided with a plurality of brush holes, the number of brush holes being the same as the number of brush bristles, and arranged opposite to each other.

[0022] One end of the brush bristles is fixedly connected to the inner wall of the receiving box, and the other end of the brush bristles passes through the brush bristle hole and is located on the side of the movable base plate away from the receiving box.

[0023] Preferably, the scale tube is provided with scale lines.

[0024] Preferably, the base box has a groove, and the liquid collection box is detachably connected to the groove. The height of the liquid collection box matches the shape and size of the groove on the base box, so that the liquid collection box can be snapped into the groove on the base box.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] 1. This utility model provides a plant virus friction grafting device. The device features a movable base plate that moves within the grafting box, allowing adjustment of the bristle length on the side of the box away from the grafting box. This enables the user to easily control the friction intensity during operation, ensuring successful plant grafting. This friction grafting device allows experimenters to successfully graft the virus after only one preliminary adjustment, eliminating the need for careful control of friction intensity. The entire device integrates virus sap collection and friction grafting, achieving highly efficient friction grafting.

[0027] 2. The friction inoculation device provided by this utility model can strictly control the frictional force on the leaf, and the force is evenly distributed across the entire leaf, avoiding insufficient friction or excessive force during inoculation. It is suitable for use by laboratory novices and can reduce the number of experimental trials and failures.

[0028] 3. The friction-contact poison device provided by this utility model has the advantages of high efficiency, short time, simple structure, and convenient and quick operation in the laboratory.

[0029] 4. The friction-based poison-collecting device provided by this utility model has a movable base plate and a scale tube. The movable base plate moves up and down in the body of the poison-collecting box through the scale tube to control the length of the bristles, so as to achieve the bristle length that does not damage the blades and can successfully complete the poison collection. Attached Figure Description

[0030] Figure 1 This is a schematic diagram of the overall structure of the friction-based poison-contact device in Embodiment 1 of this utility model, wherein... Figure 1 A in the diagram is a structural schematic of the receiving box and the bottom box; Figure 1 B in the diagram is a schematic diagram of the liquid collection box;

[0031] Figure 2 This is a schematic diagram of the structure of the receiving box in Embodiment 1 of this utility model;

[0032] Figure 3 This is a schematic diagram of the split structure of the receiving box in Embodiment 1 of this utility model;

[0033] Figure 4 This is a top view of the U-shaped cylindrical connecting block in Embodiment 1 of this utility model;

[0034] Figure 5 This is a schematic diagram of the scale tube in Embodiment 1 of this utility model;

[0035] Figure 6 This is a partially enlarged structural diagram of part A in this utility model;

[0036] Figure 7The results are PCR test results after inoculation with the friction inoculation device of Example 1; where Line 1-10 are inoculated with *Fumiganthus benthamianus*, Line 11 is the template-free control, and Line 12 is the positive control; M is DL2000: 100, 250, 500, 750, 1000, 2000 bp.

[0037] Figure 8 The results of PCR testing after artificial friction infection are shown in Comparative Example 1; Lines 1-10 are the results of infection with *Fumiganthus benthamianus*, and M is DL2000: 100, 250, 500, 750, 1000, 2000 bp.

[0038] In the diagram: 1-liquid collection box, 2-poison collection box, 201-box body, 202-movable base plate, 2021-brush hole, 2022-pipe hole, 2023-cylindrical with hole, 203-brush bristles, 204-gradient tube, 2041-disc, 2042-connecting tube, 20421-connecting protrusion, 20422-gradient line, 205-U-shaped cylindrical connecting block, 2051-connecting groove, 3-base box, 301-groove, and 4-box body hole. Detailed Implementation

[0039] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0040] In the description of this utility model, it should be understood that the terms "upper", "lower", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0041] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0042] Example 1

[0043] A plant virus friction inoculation device, see [link to documentation]. Figures 1-6It includes a liquid collection box 1 and a friction contact assembly; the liquid collection box 1 and the friction contact assembly are detachably connected.

[0044] The friction inoculation assembly includes a base box 3, which is connected to an inoculation box 2 via a standard hinge, forming an openable and closable box structure. The inoculation box 2 can be closed onto the base box 3, creating a closed space between them. When the inoculation box 2 is connected to the base box 3 and is not closed, there is a certain distance and space between them, with the top of the base box 3 uncovered, effectively creating an open box top. The base box 3 is used to hold the liquid collection box 1 or to support the test leaf. The inoculation box 2 is used to actuate the test leaf for friction inoculation with plant viruses.

[0045] The base box 3 has a groove 301, on which a liquid collection box 1 is detachably connected. The liquid collection box 1 can be snapped onto the base box 3. The height of the liquid collection box 1 matches the shape and size of the groove 301 on the base box 3, allowing the liquid collection box 1 to snap into the groove 301 on the base box 3. The liquid collection box 1 is positioned opposite to the virus collection box 2. The liquid collection box 1 is used to hold the viral fluid. The base box 3 serves to support the test leaf. In use, when the virus collection box 2 is connected to the base box 3 and is not closed, first snap the liquid collection box 1 into the groove 301 on the base box 3, then collect the viral fluid, and then place the collected viral fluid into the liquid collection box 1. Finally, close the virus collection box 2 onto the base box 3. At this point, the collection box 2 will come into contact with the viral fluid. Then, the collection box 1 is removed from the groove 301 on the base box 3, the test leaf is placed into the base box 3, and then the collection box 2 is closed on the base box 3. At this time, the collection box 2, which is in contact with the viral fluid, will come into contact with the test leaf placed on the base box 3, and then the frictional viral collection of the test leaf can be achieved by controlling other structures of the collection box 2.

[0046] The virus collection box 2 includes a box body 201. A plurality of bristles 203 are fixedly arranged on the wall of the box body 201. The bristles 203 are perpendicular to or inclined relative to the liquid collection box 1, and the length of the bristles 203 is equal to the depth of the liquid collection box 1. A movable base plate 202 is also movably arranged on the inner wall of the box body 201. The movable base plate 202 can move towards or away from the liquid collection box 1. One end of the bristles 203 away from the inner wall of the box body 201 moves through the movable base plate 202. When the movable base plate 202 moves towards or away from the liquid collection box 1, the movable base plate 202 moves relative to the bristles 203. In use, when the virus collection box 2 is connected to the base box 3 and is in an open state, the liquid collection box 1 is first snapped into the groove 301 on the base box 3, then the viral fluid is collected, and then the collected viral fluid is placed in the liquid collection box 1. Finally, the virus collection box 2 is closed onto the base box 3. At this point, the bristles 203 on the inoculation box 2 will come into contact with the virus sap. The sap collection box 1 is then removed from the groove 301 on the base box 3, and the test leaf is placed into the base box 3. The inoculation box 2 is then closed onto the base box 3. The bristles 203, now in contact with the virus sap, will then come into contact with the test leaf placed on the base box 3. The movable base plate 202 moves within the box body 201, allowing adjustment of the length of the bristles 203 on the side of the movable base plate 202 furthest from the box body 201. This controls the degree of contact between the bristles 203 and the test leaf, making it easy for the user to control the friction force during operation and ensuring successful plant inoculation. This friction inoculation device provides strict control over the friction force on the leaf, ensuring uniform force across the entire leaf and preventing insufficient or excessive friction during inoculation. It is suitable for laboratory novices, reducing the number of trial and error attempts and failures.

[0047] Furthermore, the bristles 203 are tufted bristles 203. The bristles 203 are set perpendicular to the movable base plate 202. The purpose is to change the effective length of the bristles 203 by moving the base plate 202, thereby changing the flexibility of the bristles to adapt to plant leaf surfaces with different softness, improving inoculation efficiency and reducing plant damage.

[0048] Furthermore, a scale tube 204 is movably connected to the inner wall of the box 201. One end of the scale tube 204 passes through the box 201, and the other end passes through and is rigidly connected to the movable base plate 202. By adjusting the scale tube 204, the effective length of the brush bristles 203 can be set to quantify the application force, thus adapting to the different softness of the leaves of different plants.

[0049] Furthermore, the movable base plate 202 is provided with a pipe hole 2022. One end of the graduated tube 204, away from the inner wall of the box 201, passes through the pipe hole 2022 and is located on the side of the movable base plate 202 away from the box 201. The movable base plate 202 moves up and down within the box 201 via the graduated tube 204 to control the length of the bristles 203, achieving a bristle length that neither damages the blades nor hinders successful application of the disinfectant. Controlling the bristle length through these devices solves the problem of difficult-to-control friction in previous devices, improving the standardization and efficiency of personnel work.

[0050] Furthermore, the movable base plate 202 is also provided with a pair of symmetrically arranged cylindrical perforations 2023. The distance between the two cylindrical perforations 2023 is greater than the diameter of the pipe hole 2022, and the two cylindrical perforations 2023 are located on both sides of the pipe hole 2022. The purpose is to allow 204 to pass through smoothly and to control its movement.

[0051] Furthermore, the end of the scale tube 204 away from the inner wall of the box 201 is detachably connected to a U-shaped cylindrical connecting block 205 through the pipe hole 2022. The cylindrical connecting block 205 passes through and penetrates the pipe hole 2022 and is rigidly connected to the inner plane of the box 201. The groove is closed, so that the movable base plate 202 driven by the scale tube 204 is kept in the closed groove formed by the cylindrical connecting block 205 and the inside of the box 201 and moves longitudinally, thereby adjusting the longitudinal distance of the movable base plate 202 and thus controlling the effective length of the bristles 203.

[0052] The open end of the cylindrical connecting block 205 passes through the cylindrical perforated 2023 from bottom to top and is movably connected to the cylindrical perforated 2023. Further, after passing through the cylindrical perforated 2023, the cylindrical connecting block 205 is rigidly connected to the inner wall of the box 201. This allows the cylindrical connecting block 205 and the graduated tube 204 to form a piston-like structure, enabling 204 to slide within 205.

[0053] Furthermore, the cylindrical connecting block 205 is provided with a connecting groove 2051; the end of the scale tube 204 away from the inner wall of the box body 201 is provided with a connecting protrusion 20421; the connecting protrusion 20421 is used to snap into the connecting groove 2051, so as to achieve a rigid connection between the cylindrical connecting block 205 and the box body 201. In use, when the virus collection box 2 is connected to the base box 3 and is in an open state, first snap the liquid collection box 1 into the groove 301 on the base box 3, then collect the virus juice, and then place the collected virus juice into the liquid collection box 1. Then close the virus collection box 2 onto the base box 3. At this time, the bristles 203 provided on the virus collection box 2 will come into contact with the virus juice. Then remove the liquid collection box 1 from the groove 301 on the base box 3, put the test leaf into the base box 3, and then close the virus collection box 2 onto the base box 3. At this time, the bristles 203 that have come into contact with the virus sap will come into contact with the test leaf placed on the base box 3. Then, the movable base plate 202 will move up and down in the box body 201 through the scale tube 204 to control the length of the bristles 203, that is, to control the length of the bristles 203 that come into contact with the test leaf. This controls the degree of contact between the bristles 203 and the test leaf, so that the user can easily control the friction force in actual operation and ensure the normal progress of plant inoculation.

[0054] Furthermore, the scale tube 204 includes a disc 2041 and a connecting tube 2042, with one end of the connecting tube 2042 connected to the disc 2041. The housing 201 is provided with a housing hole 4, the diameter of which is similar to that of the 2042, allowing the connecting tube 2042 to move longitudinally within the hole.

[0055] The other end of the connecting pipe 2042 passes through the box hole 4 and the pipe hole 2022 in sequence, and is snapped into the connecting groove 2051 by the connecting protrusion 20421, so that the connecting pipe 204 is rigidly connected to the movable base plate 202.

[0056] Furthermore, the movable base plate 202 is also provided with a number of brush holes 2021. The number of brush holes 2021 is the same as the number of brushes 203, and they are arranged relative to each other. The brushes 203 can move freely within the brush holes 2021.

[0057] One end of the bristle 203 is fixedly connected to the inner wall of the box 201, and the other end of the bristle 203 passes through the bristle hole 2021, located on the side of the movable base plate 202 away from the box 201. In the direction away from the inner wall of the box 201, the length of the bristles between the inner wall of 201 and the movable base plate 202 is the ineffective length; the remaining bristles outside the movable base plate 202 are the effective length. Adjusting the effective length of the bristles controls their softness; the shorter the effective length, the greater the rigidity, suitable for leaves with relatively hard surfaces.

[0058] Further, a scale line 20422 is provided on the connecting pipe 2042. By adjusting the scale line 20422, the effective length of the bristles, i.e., the softness, can be controlled.

[0059] The usage process of this device is as follows:

[0060] 1. Virus sap collection

[0061] Cut the fresh or ultra-low temperature preserved virus-infected leaves into small pieces about 0.5 cm, put them into a sterilized mortar, add a small amount of 500-mesh carborundum in the mortar to assist grinding, and homogenize on ice in a 0.02 mol / L phosphate buffer solution with the mortar. Transfer the well-homogenized virus sap to the liquid extraction box 1 in this friction inoculation device.

[0062] 2. Friction inoculation

[0063] Since the leaf thicknesses of different test plants are different, adjust different scales and move the test plant leaves up and down three times, observe the damage conditions of the test plant leaves, and select an appropriate scale of the bristles 203 for subsequent friction inoculation.

[0064] The specific steps are to fully immerse the bristles 203 in the liquid extraction box 1 in the inoculation box 2, place the test plant leaves on the bottom box 3, and move the inoculation box 2 from back to front three times to complete the inoculation.

[0065] To further illustrate the effect of the above plant virus friction inoculation device provided by the present utility model, taking the inoculation of Nicotiana benthamiana leaves with Melon yellow spot virus (MYSV) as an example, the following tests were carried out:

[0066] Application example

[0067] A method for detoxifying using the friction inoculation device of Example 1 includes the following steps:

[0068] 1. Virus sap collection

[0069] Take 500 mg of the previously inoculated and MYSV-carrying tobacco leaves, cut them into small pieces of 0.5 cm size with sterilized scissors, put them into a pre-cooled mortar, add a small amount of 500-mesh carborundum to assist grinding, add 2 mL of pre-cooled PBS buffer solution with a pH of 7.3 ± 1 and a concentration of 0.02 mol / L, and quickly grind on ice to obtain virus sap. Then, transfer half of the virus sap to the liquid extraction box 1 that is配套 with the friction inoculation device of Example 1.

[0070] 2. Friction inoculation of Nicotiana benthamiana leaves

[0071] It should be noted that the phrase "配套 with" in the translation of item might need to be adjusted according to the actual context to make it more accurate and natural, such as "matched with" or "compatible with". Also, the text seems to be a bit incomplete at the end of item where the full description after "配套 with" is not provided.Inoculation device: Since the leaves of Nicotiana benthamiana are relatively soft, the brush bristles 203 with a scale of 3.5cm are selected. The brush bristles 203 of the inoculation box 2 in the friction inoculation device of Example 1 are fully moistened in the liquid collection box 1. The leaves of the test plant are placed on the bottom box 3, and the inoculation box 2 is moved from top to bottom three times to complete the inoculation. A total of 10 Nicotiana benthamiana plants are inoculated.

[0072] Comparative Example 1

[0073] A method for artificially applying toxins through friction includes the following steps:

[0074] 1. Collection of viral fluid

[0075] Take 500 mg of tobacco leaves previously successfully inoculated with MYSV, cut them into 0.5 cm pieces using sterilized scissors, place them in a pre-chilled mortar, add a small amount of 500-mesh diamond abrasive to assist grinding, add 2 mL of 0.02 mol / L pre-chilled PBS buffer (pH 7.3±1), and grind rapidly on ice to obtain the viral extract. Then, leave half of the viral extract in the mortar.

[0076] 2. Two methods for inoculating Benedict's tobacco leaves with rubbing agents.

[0077] After putting on gloves, dip your fingers into the virus juice in the mortar and gently rub the leaves of Nicotiana benthamiana from top to bottom three or more times to complete the inoculation. A total of 10 Nicotiana benthamiana plants were inoculated.

[0078] To compare the effectiveness of the detoxification method using the friction-applied poisoning device of Example 1 and the manual friction-applied poisoning method of Comparative Example 1, this invention uses the MYSV detection method to perform MYSV detection on the poisoned Benedictine tobacco leaves. The specific steps are as follows:

[0079] Fourteen days after inoculation, 100 mg of tobacco leaves were placed in a 1.5 mL RNase-free centrifuge tube, flash-frozen in liquid nitrogen, and total RNA was extracted from the leaves using the TRIzol method. The extracted total RNA was reverse transcribed into cDNA using TAKARAR0047A, and then detected by RT-PCR using MYSV-specific primers: MYSV-F and MYSV-R.

[0080] A 25 μL mixture of Novizan 2×Rapid-Taq Mix (12.5 μL), primers (1 μL each), cDNA (2 μL), and dH2O (8.5 μL) was prepared. The mixture was pre-deformed at 98 °C for 3 min, followed by 30 amplification cycles at 98 °C for 15 s, 58 °C for 15 s, and 72 °C for 30 s, with a final extension at 72 °C for 5 min. Electrophoresis was performed on a 1.5% agarose gel at 110 V for 20 min, and images were obtained under UV excitation. The band at the 500 bp position was observed.

[0081] The nucleotide sequence of MYSV-F is 5'-GACAACAGGGCAGAGCGAATG-3'. The nucleotide sequence of MYSV-R is 5'-TACCGTTACTAAGCTGACAAAGGAGAA-3'.

[0082] Test results as follows Figure 7 and Figure 8 As shown.

[0083] The results showed that 9 strains of *Flavorum benthamianum* were successfully inoculated using the friction inoculation device of Example 1, while 5 strains were successfully inoculated using the manual friction inoculation method of Comparative Example 1. This indicates that the inoculation efficiency of the friction inoculation device provided by this invention is 1.8 times that of manual inoculation.

[0084] This invention features a movable base plate that moves within the container, allowing adjustment of the bristle length on the side of the base plate furthest from the container. This enables users to easily control the friction intensity during operation, ensuring successful plant grafting. The friction grafting device provided by this invention can precisely control the friction force on the leaves, ensuring uniform force across the entire leaf and preventing insufficient or excessive friction during inoculation. It is suitable for laboratory novices, reducing the number of trial and error attempts and failures.

[0085] The above-disclosed embodiments are merely specific examples of this utility model, but the embodiments of this utility model are not limited thereto. Any variations that can be conceived by those skilled in the art should fall within the protection scope of this utility model.

Claims

1. A plant virus friction inoculation device, characterized in that, It includes a liquid collection box (1) and a friction-contacting component; the liquid collection box (1) and the friction-contacting component are detachably connected; The friction-to-discharge assembly includes a base box (3), a receiving box (2) is movably connected to the base box (3), and a liquid collection box (1) is detachably connected to the base box (3). The liquid collection box (1) and the receiving box (2) are arranged opposite to each other. The inner wall of the receiving box (2) is provided with a number of bristles (203), the length of which is equal to the depth of the liquid collection box (1); The receiving box (2) is provided with a movable base plate (202), which can move toward or away from the liquid collection box (1), and the bristles (203) move through the movable base plate (202). A connecting pipe is connected to the movable base plate (202), and the connecting pipe passes through the poison receiving box (2).

2. The plant virus friction inoculation device according to claim 1, characterized in that, The inner wall of the receiving box (2) is connected to a grooved cylindrical connecting block (205), and the cylindrical connecting block (205) and the connecting tube together form a movable piston structure.

3. The plant virus friction inoculation device according to claim 2, characterized in that, The movable base plate (202) is provided with a pipe hole (2022), and one end of the connecting pipe away from the inner wall of the receiving box (2) passes through the pipe hole (2022) and is located on the side of the movable base plate (202) away from the receiving box (2).

4. The plant virus friction grafting device according to claim 3, characterized in that, The movable base plate (202) is also provided with a pair of symmetrically arranged cylindrical holes (2023), the distance between the two cylindrical holes (2023) is greater than the diameter of the pipe hole (2022), and the two cylindrical holes (2023) are located on both sides of the pipe hole (2022).

5. A plant virus friction grafting device according to claim 4, characterized in that, The end of the connecting pipe away from the inner wall of the receiving box (2) passes through the pipe hole (2022) and is detachably connected to the cylindrical connecting block (205). The open end of the cylindrical connecting block (205) passes through the cylindrical perforated strip (2023) and is detachably connected to the cylindrical perforated strip (2023).

6. A plant virus friction grafting device according to claim 5, characterized in that, The cylindrical connecting block (205) is provided with a connecting groove (2051); the end of the connecting pipe away from the inner wall of the receiving box (2) is provided with a connecting protrusion (20421); the connecting protrusion (20421) is used to be engaged in the connecting groove (2051).

7. A plant virus friction grafting device according to claim 6, characterized in that, The connecting tube is a graduated tube (204).

8. A plant virus friction inoculation device according to claim 7, characterized in that, The graduated tube (204) includes a disc (2041) and a connecting tube (2042). One end of the connecting tube (2042) is connected to the disc (2041), and the other end of the connecting tube (2042) passes through the wall of the receiving box (2) and the pipe hole (2022) in sequence, and is engaged in the connecting groove (2051) by the connecting protrusion (20421).

9. A plant virus friction inoculation device according to claim 1, characterized in that, The movable base plate (202) is also provided with a plurality of brush holes (2021), the number of brush holes (2021) being the same as the number of brush bristles (203) and being arranged opposite to each other; One end of the bristles (203) is fixedly connected to the inner wall of the receiving box (2), and one end of the bristles (203) passes through the bristle hole (2021) and is located on the side of the movable base plate (202) away from the receiving box (2).

10. A plant virus friction inoculation device according to claim 1, characterized in that, The bottom box (3) is provided with a groove (301), and the liquid collection box (1) is detachably connected to the groove (301).

Images