Culture device for stem cell extraction

Abstract

The utility model relates to a culture device for extracting stem cells, which is characterized by comprising a box body provided with a plurality of mounting shells, and stored samples can be inserted into the mounting shells. The installation shell is of a double-layer structure, and a heating pipe is spirally fixed in an interlayer of the installation shell. Circularly flowing liquid is poured into the heating pipe, and the liquid temperature is ensured to meet the expectation, so that the close-range layout of the heat source and the sample storage space is realized. According to the design, the heat loss is obviously reduced, the energy consumption is reduced, and the workload of the heating device is reduced. In addition, the structure can accelerate the heating process of the sample and ensure that the sample quickly reaches the required storage temperature.

Description

TECHNICAL FIELD

[0001] The utility model relates to a storage device technical field especially relates to a culture device for stem cell extraction. BACKGROUND

[0002] Stem cells are a kind of cells with unlimited or eternal self-renewal potential, which can differentiate into at least one highly specialized daughter cell.

[0003] At present, the culture equipment used for stem cell extraction is usually used as a sample storage device, which must maintain the constant temperature state of the internal stem cell sample. Therefore, the current stem cell culture device is usually equipped with a heating element.

[0004] Specifically, the device in the prior art usually uses a heating wire to realize the overall temperature rise in the storage box body, which has simple structure and ensures that the storage box body has a large enough space to accommodate more samples. However, the larger the volume of the storage cavity, the slower the internal temperature rises, which leads to the need to start the heating device in advance in actual operation, and then put the sample into after the temperature reaches the appropriate state.

[0005] At the same time, the larger the area that needs to be heated, the higher the performance requirement of the heating device, and the larger the corresponding energy consumption.

[0006] In view of this, the present research proposes a new type of culture device for stem cell extraction, which aims to reduce energy consumption and rapidly raise the temperature inside the storage box body to the required level. CONTENT OF THE UTILITY MODEL

[0007] In view of the deficiencies of the existing technology, the utility model provides a culture device for stem cell extraction. The device has the characteristics of rapid heating and low energy consumption, effectively solving the problems of large heating area, slow heating speed and high energy consumption in the existing technology.

[0008] In order to achieve the above purpose, the utility model adopts the following technical scheme:

[0009] The utility model provides a culture device for stem cell extraction, including box, be provided with heating device inside the box, be provided with storage cavity, water storage cavity, installation cavity in the box, wherein, water storage cavity with installation cavity set up in the lower end of storage cavity, heating device set up inside water storage cavity, be provided with circulating pump inside installation cavity, and circulating pump water inlet is connected with circulating pipe, and, the water inlet and water outlet of water storage cavity are opened, and circulating pump water inlet is in communication with water storage cavity water outlet, be provided with a plurality of installation casing in the storage cavity, and every installation casing is axial, and every installation casing is double -deck structure, and the interlayer of every installation casing is provided with heating pipe, and heating pipe spiral winding is with inside installation casing, and heating pipe one end is connected with circulating pipe, and heating pipe other end is in communication with water storage cavity water inlet.

[0010] Preferably, the lower end of each installation casing is provided with a ball valve, the water inlet of each ball valve is in communication with the circulating pipe, and the water outlet of each ball valve is in communication with the water inlet of the heating pipe.

[0011] Preferably, the lower end of each installation casing is coaxially provided with a sleeve, and the outer side wall of each sleeve is fixedly connected with a positioning rod, the inner wall of each installation casing is provided with a positioning hole corresponding to the positioning rod, the positioning rod is inserted into the positioning hole and moves up and down linearly, a control ring is inserted into the inside of each sleeve, the lower end of the control ring is fixedly connected with the rotating pivot of the ball valve, and the outer side wall of each control ring is provided with a plurality of follow-up grooves, the inner side wall of each sleeve is fixedly connected with a plurality of driven rods corresponding to the follow-up grooves, the driven rods are inserted into the corresponding follow-up grooves, the upper and lower ends of each follow-up groove are not in the same vertical plane, and the inner wall of each follow-up groove is smoothly transitioned.

[0012] Preferably, the inside of each control ring is fixedly connected with an up-and-down axial telescopic rod, the fixed end of the telescopic rod is fixedly connected with the control ring, the movable end of the telescopic rod is fixedly connected with the inner wall of the sleeve, a compression spring is sleeved outside each telescopic rod, and the two axial ends of the compression spring are respectively abutted with the corresponding control ring and sleeve.

[0013] Preferably, the upper end of the box is detachably provided with a box cover, when the sample is placed in the installation casing and the upper end inner wall of the box cover is abutted with the upper end surface of the box, the corresponding compression spring of the installation casing is in a compressed state, and the corresponding ball valve of the installation casing is in an open state.

[0014] Compared with the prior art, the utility model has the beneficial effects that:

[0015] The utility model discloses a double -layer shell structure, namely installation shell body, through the configuration heating pipe in the interlayer of installation shell body, realized the close -range layout of heat source and sample storage space. This design effectively reduces the heat loss, reduces the energy consumption, and alleviates the work load of heating device. In addition, the structure can also accelerate the sample heating process, ensure that the sample reaches the required storage temperature rapidly. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 It is the whole structure schematic diagram of the utility model.

[0017] Figure 2 It is the box internal structure schematic diagram of the utility model.

[0018] Figure 3 It is the heating pipe and ball valve connection schematic diagram of the utility model.

[0019] Figure 4 It is the sleeve and installation shell body connection schematic diagram of the utility model.

[0020] Figure 5 It is the sleeve and control ring cooperation relationship schematic diagram of the utility model.

[0021] In the drawing: 1, box cover;2, box body;3, installation shell body;4, heating pipe;5, heating device;6, circulating pump;7, circulating pipe;8, ball valve;9, positioning rod;10, sleeve;11, positioning hole;12, control ring;13, follow-up groove;14, telescopic rod;15, compression spring;16, driven rod. DETAILED DESCRIPTION

[0022] The technical scheme in the embodiments of the utility model will be described clearly and completely below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the utility model.

[0023] In the description of the utility model, it is understood that the terms "up", "down", "front", "back", "left", "right", "top", "bottom", "in", "out" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, therefore it cannot be understood as a limitation on the utility model.

[0024] Please refer to Figures 1-5A culture device for stem cell extraction, which is consistent with the prior art device, comprises a box 2. In addition, the box 2 is specially equipped with a heating device 5 to ensure the heat preservation effect of the storage content.

[0025] The device is different from the prior art device in that the box 2 is specially designed with a storage cavity, a water storage cavity and a mounting cavity. The water storage cavity and the mounting cavity are cleverly arranged at the lower end of the storage cavity.

[0026] It should be pointed out that the heating device 5 is precisely embedded in the water storage cavity. The water storage cavity is filled with liquid, which enables the heating device 5 to heat the liquid in the cavity and ensure that the liquid temperature is constant at a preset value.

[0027] It is particularly emphasized that the device is configured with a plurality of mounting shells 3 arranged along the upper and lower axes in the storage cavity, and each shell has a double-layer structure. This design allows a heating pipe 4 to be arranged in the interlayer of each shell. When the constant-temperature liquid in the water storage cavity flows into the heating pipe 4, the temperature of the stored sample can be quickly stabilized, and the air resistance to heat transfer is reduced.

[0028] Specifically, the device is fixedly connected with the box 2 to ensure that the position of the mounting shell 3 remains unchanged relative to the box 2.

[0029] Further, each heating pipe 4 is spirally wound on the inner side of the corresponding mounting shell 3. This layout increases the heat radiation range of the liquid in the heating pipe 4 to the stored sample, further improves the heat preservation effect of the sample, and ensures that the sample can quickly reach a constant temperature state.

[0030] Further, as shown in Figure 1 The device is filled with temperature insulation cotton around the mounting shells 3, which can limit the direction of heat dissipation of the heating pipe 4 and ensure that the liquid in the heating pipe 4 can fully heat and preserve the stored sample.

[0031] One end of the water storage cavity is provided with a water inlet hole, and the other end is provided with a water outlet hole. The device is configured with a circulating pump 6 in the mounting cavity. The water outlet of the circulating pump 6 is connected with the water inlet of the heating pipe 4 through a circulating pipe 7, and the water inlet of the circulating pump 6 is connected with the water outlet hole of the water storage cavity. The water inlet hole of the water storage cavity is connected with the water outlet of the heating pipe 4, so that the liquid in the heating pipe 4 can flow continuously through the circulating pipe 7. In this way, the liquid in the heating pipe 4 can be replaced, and the heated liquid can continuously flow into the heating pipe 4 to heat the stored sample.

[0032] Further, the device is configured with a ball valve 8 at the lower end of each installation shell 3. The water inlet of the ball valve 8 is connected with the circulating pipe 7, and the water outlet is connected with the heating pipe 4. This structure design can effectively control the flow direction of the liquid in the circulating pipe 7 through the opening and closing operation of the ball valve 8, prevent heat loss, and only make the heated liquid continuously flow in the heating pipe 4 corresponding to the installation shell 3 storing the sample.

[0033] In order to realize the precise control of the opening and closing of the ball valve 8, the device is coaxially configured with a sleeve 10 at the lower end of each installation shell 3. A positioning rod 9 is fixedly connected to the outer side wall of each sleeve 10, and a positioning hole 11 is formed in the inner wall of each installation shell 3 corresponding to the positioning rod 9. The positioning rod 9 can be inserted into the positioning hole 11, and the constraint of the positioning hole 11 on the positioning rod 9 ensures that the sleeve 10 can only move linearly up and down.

[0034] In addition, a control ring 12 is inserted into each sleeve 10, and the lower end of the control ring 12 is fixedly connected with the rotating pivot of the ball valve 8. By rotating the control ring 12, the opening and closing of the ball valve 8 can be controlled.

[0035] Therefore, a plurality of follow-up grooves 13 are formed in the outer side wall of each control ring 12. A plurality of driven rods 16 are fixedly connected to the inner side wall of each sleeve 10 corresponding to the follow-up grooves 13. By inserting the driven rod 16 into the corresponding follow-up groove 13, and the upper and lower ends of each follow-up groove 13 are not in the same vertical plane, and the inner wall of each follow-up groove 13 is smoothly transitioned, the contact position of the driven rod 16 and the follow-up groove 13 can be changed when the sleeve 10 moves up and down relative to the control ring 12, so that the rotation of the control ring 12 is realized through the sleeve 10, and the opening and closing of the ball valve 8 is further controlled.

[0036] Further, the device is provided with a cover 1 at the upper end of the box 2. When the sample is stored in the installation shell 3 and the inner wall of the upper end of the cover 1 is in close contact with the upper end of the box 2, the compression spring 15 in the installation shell 3 will be in a compressed state, and the corresponding ball valve 8 will be in an open state. Therefore, after the daily storage task is completed, the sample and the device need to be properly stored, and the existence of the cover 1 will automatically apply downward pressure to the sample, so as to move the sample downward, thereby providing the required downward pressure for the sleeve 10.

[0037] It must be pointed out that in actual application, corresponding locking mechanisms are arranged between the cover 1 and the box 2, which can be clamping mechanisms, locking mechanisms, etc. Since these belong to the category of prior art, the device will not be described in detail.

[0038] Further, to ensure that the sleeve 10 can be automatically reset and drive the ball valve 8 to close after the sample is taken out from the installation shell 3, the device is configured with a compression spring 15 between the sleeve 10 and the control ring 12. The two ends of the spring are in contact with the control ring 12 and the sleeve 10 respectively, so as to provide stable elastic force and ensure that the sleeve 10 and the control ring 12 have opposite movement trends.

[0039] Further, to ensure that the center axis of the compression spring 15 is always perpendicular to the center axis of the control ring 12 and the sleeve 10, so as to be able to exert vertical thrust on the sleeve 10 and the control ring 12, the device is fixedly connected with an up-down axial telescopic rod 14 inside each control ring 12. The fixed end of the telescopic rod 14 is fixedly connected with the control ring 12, and the movable end is fixedly connected with the inner wall of the sleeve 10, which ensures the stability and accuracy of the control ring 12.

[0040] In the actual application process of the utility model,

[0041] Firstly, the operator places the encapsulated sample in the corresponding installation shell 3, at this time, the heating device 5 is started synchronously, and the temperature of the liquid in the water storage cavity gradually rises;

[0042] Then, the operator installs the box cover 1 and cooperates with the corresponding locking mechanism to limit the positional relationship between the box cover 1 and the box body 2, at the same time, the circulating pump 6 is started, the sample storage drives the sleeve 10 to move downward, the control ring 12 rotates, the sample storage corresponding ball valve 8 is opened, and the liquid in the water storage cavity gradually flows into the heating pipe 4, and the temperature of the liquid in the heating pipe 4 reaches the expected state;

[0043] Finally, when the operator needs to take out the sample storage, the box cover 1 is opened, the elastic potential energy of the compression spring 15 is released, and the sample storage is ejected from the inside of the installation shell 3, and the sample storage corresponding ball valve 8 is closed.

[0044] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A culture device for stem cell extraction, comprising a box (2) provided with a heating device (5) inside, characterized in that: The box (2) is internally provided with a storage cavity, a water storage cavity and a mounting cavity, wherein the water storage cavity and the mounting cavity are arranged at the lower end of the storage cavity, and the heating device (5) is arranged inside the water storage cavity; The mounting cavity is internally provided with a circulating pump (6), the water outlet of the circulating pump (6) is connected with a circulating pipe (7), and the water storage cavity is provided with a water inlet hole and a water outlet hole, and the water inlet of the circulating pump (6) is communicated with the water outlet hole of the water storage cavity; The storage cavity is internally provided with a plurality of mounting shells (3), and each mounting shell (3) is vertically arranged; A heating pipe (4) is arranged in the interlayer of each mounting shell (3), the heating pipe (4) is spirally wound in the mounting shell (3), and one end of the heating pipe (4) is connected with the circulating pipe (7), and the other end of the heating pipe (4) is communicated with the water inlet hole of the water storage cavity.

2. The culture device for stem cell extraction according to claim 1, wherein: Each mounting shell (3) is provided with a ball valve (8) at the lower end, the water inlet of each ball valve (8) is communicated with the circulating pipe (7), and the water outlet of each ball valve (8) is communicated with the water inlet of the heating pipe (4).

3. The culture device for stem cell extraction according to claim 2, wherein: Each mounting shell (3) is coaxially provided with a sleeve (10) at the lower end, and each sleeve (10) is fixedly connected with a positioning rod (9) on the outer side wall, and a positioning hole (11) is formed in the inner wall of each mounting shell (3) for the positioning rod (9), and the positioning rod (9) is inserted into the positioning hole (11) and moves up and down linearly; Each sleeve (10) is inserted with a control ring (12), the lower end of the control ring (12) is fixedly connected with the rotating pivot of the ball valve (8), and a plurality of follow-up grooves (13) are formed in the outer side wall of each control ring (12), and a plurality of driven rods (16) are fixedly connected to the inner side wall of each sleeve (10) for the follow-up grooves (13), and the driven rods (16) are inserted into the corresponding follow-up grooves (13); The upper and lower ends of each follow-up groove (13) are not in the same vertical plane, and the inner wall of each follow-up groove (13) is smoothly transitioned.

4. The culture device for stem cell extraction according to claim 3, wherein: Each control ring (12) is fixedly connected with an up-down axial telescopic rod (14), the fixed end of the telescopic rod (14) is fixedly connected with the control ring (12), and the movable end of the telescopic rod (14) is fixedly connected with the inner wall of the sleeve (10); Each telescopic rod (14) is provided with a compression spring (15) outside, and the two axial ends of the compression spring (15) are respectively abutted with the corresponding control ring (12) and sleeve (10).

5. The culture device for stem cell extraction according to claim 4, wherein: The upper end of the box (2) is provided with a box cover (1), when the sample is placed in the mounting shell (3) and the upper end inner wall of the box cover (1) is abutted with the upper end surface of the box (2), the corresponding compression spring (15) of the mounting shell (3) is in a compressed state, and the corresponding ball valve (8) of the mounting shell (3) is in an open state.

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