Root-knot nematode egg grain rapid disinfection and larva separation device

Abstract

The utility model provides a root knot nematode egg grain rapid disinfection and larva separation device which comprises a water containing barrel, a nematode collecting funnel is installed at the bottom of the water containing barrel, a cut-off rubber flexible rubber hose is installed at the bottom of the nematode collecting funnel, a water stop clamp is installed on the cut-off rubber flexible rubber hose, and the water stop clamp is connected with the water containing barrel. An oocyst collecting net and an egg grain collecting net which are distributed side by side up and down at intervals are mounted in the water containing barrel. The rapid disinfection and larva separation device for the root-knot nematode eggs is simple in structure, reasonable in design and convenient to use, a large batch of clean root-knot nematode larva suspension liquid with the same insect age can be collected in a short time, and tests such as toxicity determination and agent screening of various nematode killing agents can be conveniently and rapidly completed in a laboratory.

Description

Technical Field

[0001] This utility model relates to the field of separation device technology, specifically to a device for rapid disinfection of root-knot nematode eggs and separation of larvae. Background Technology

[0002] Root-knot nematodes (Meloidogyne) are highly specialized omnivorous plant pathogenic nematodes. With the increase in protected vegetable cultivation areas, the occurrence and damage areas of root-knot nematodes have expanded rapidly, seriously impacting my country's agricultural economy. Therefore, more and more scholars have begun to study the control of root-knot nematodes. Previously, most nematode research experiments obtained root-knot nematodes by directly filtering them from nematode-infested soil using the "Bellman funnel method." This process presents three main challenges: First, the nematode suspension collected by filtration often contains other soil nematodes, plankton, and microorganisms besides root-knot nematodes, requiring significant time for nematode screening and hindering the rapid acquisition of large quantities of experimental material. Second, the directly filtered nematode suspension cannot be sufficiently and quickly sterilized, containing numerous bacteria that easily contaminate experimental materials, making subsequent experimental results unreliable. Third, the collected nematodes are of inconsistent ages, leading to poor experimental repeatability and insufficient rigor in the results. Utility Model Content

[0003] To address the shortcomings of existing technologies, this invention proposes a device for rapid disinfection of root-knot nematode eggs and separation of larvae. This device can collect a large quantity of clean, uniformly aged root-knot nematode larvae suspensions in a short time, facilitating rapid testing of the toxicity of various nematicides and agent screening in the laboratory.

[0004] To achieve the above technical solution, this utility model provides a device for rapid disinfection of root-knot nematode eggs and separation of larvae, comprising: a water tank, a nematode collection funnel installed at the bottom of the water tank, a flow-blocking rubber tube installed at the bottom of the nematode collection funnel, a water-stopping clamp installed on the flow-blocking rubber tube, and an egg sac collection net and an egg collection net arranged side by side at intervals inside the water tank.

[0005] In the above technical solution, during actual operation, researchers collect root samples from plants infected with root-knot nematodes in the field. Under a stereomicroscope, they use a dissecting needle to extract the oocysts, which are then aspirated through a pipette and injected into the oocyst collection net of the separation device to prepare for the next step of disinfection. The water-stop clamp is then closed, and 75% alcohol or other disinfectant is poured into the water tank through the top opening until the liquid level is 1 cm above the surface of the oocysts in the collection net. After gently shaking for 30 seconds, the bottom water-stop clamp is opened, and the disinfectant is drained. Then, sterile water is poured into the water tank through the top opening and continuously rinsed for 10 seconds. The water-stop clamp is then closed, and sterile water is poured into the water tank again until it covers the nematode oocysts by 1 cm, completing the initial disinfection of the oocysts. The disinfected oocysts from the previous step are then gently opened with a dissecting needle to scatter the eggs. The separation device is gently shaken, and the eggs fall onto the egg collection net under gravity. After this step, the bottom water-stop clamp is opened, and the water is drained. Then, after closing the stopcock, pour 75% alcohol or other disinfectant into the water container through the top opening until the liquid level exceeds the egg cyst collection net. Gently shake for 30 seconds, then open the bottom stopcock and drain the disinfectant. Next, pour sterile water into the container through the top opening and rinse continuously for 10 seconds. Close the stopcock again, and then pour sterile water into the water container until it covers the nematode egg cysts by 1 cm, completing the thorough disinfection of the egg cysts and eggs. After thorough disinfection using the above steps, place the entire separation device in a dark environment and wait for a large number of second-instar nematode larvae to hatch. Under the influence of gravity, the nematode larvae gradually gather at the bottom of the nematode collection funnel. After one day, gently open the bottom stopcock, and release 10-20 ml of the nematode suspension from the bottom into the nematode culture dish through the intercepting rubber tubing. This will yield a large number of clean, sterile second-instar nematode larvae of uniform age, ready for use in various research experiments.

[0006] Preferably, the density of the egg sac collecting net is less than that of the egg grain collecting net to facilitate the separation of egg sacs and eggs.

[0007] Preferably, the egg sac collection net is a 200-mesh stainless steel filter and the egg collection net is a 500-mesh stainless steel filter. In actual experiments, it was found that when the egg sac collection net is a 200-mesh stainless steel filter and the egg collection net is a 500-mesh stainless steel filter, the egg sac, egg and larva can be well separated, and the experimental results are good.

[0008] Preferably, the water container is made of corrosion-resistant transparent glass or transparent plastic, which can be disinfected and reused multiple times, and facilitates observation of the internal water level and the condition of egg sacs, eggs, and larvae.

[0009] Preferably, the nematode collection funnel is made of corrosion-resistant transparent glass or transparent plastic, which can be disinfected and reused multiple times, and facilitates observation of the internal water level and the condition of egg sacs, eggs, and larvae.

[0010] The beneficial effects of the root-knot nematode egg rapid disinfection and larval separation device provided by the utility model are as follows:

[0011] (1) This separation device has a simple structure, and the materials are readily available, the cost is low, and it is easy to use.

[0012] (2) This separation device can quickly and thoroughly disinfect the collected nematode oocysts and eggs to prevent the growth of miscellaneous bacteria during the later research experiments from interfering with the experimental results and to improve the success rate of the research experiments.

[0013] (3) This separation device can collect nematode larvae of uniform species, saving a lot of time in screening nematode larvae species.

[0014] (4) This separation device can collect a large number of nematode larvae in a short time, and can achieve uniform larval age, resulting in better experimental repeatability and higher rigor of experimental results, providing more reliable research data for later scientific research. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural diagram of the present invention.

[0016] In the diagram: 1. Egg sac collection net; 2. Egg collection net; 3. Nematode collection funnel; 4. Intercepting rubber hose; 5. Water stop clamp; 6. Water container. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0018] Example: A device for rapid disinfection of root-knot nematode eggs and separation of larvae.

[0019] Reference Figure 1 As shown, a device for rapid disinfection of root-knot nematode eggs and separation of larvae includes: an egg sac collection net 1, an egg collection net 2, a nematode collection funnel 3, a flow-blocking rubber tube 4, a water-stopping clamp 5, and a water-holding tank 6. The nematode collection funnel 3 is installed at the bottom of the water-holding tank 6, the flow-blocking rubber tube 4 is installed at the bottom of the nematode collection funnel 3, and the water-stopping clamp 5 is installed on the flow-blocking rubber tube 4 to control the opening and closing of the flow-blocking rubber tube 4. The egg sac collection net 1 and the egg collection net 2 are installed side by side at intervals inside the water-holding tank 6.

[0020] In this embodiment, the density of the egg sac collection net 1 is less than that of the egg grain collection net 2 to facilitate the separation of egg sacs and eggs. Specifically, the egg sac collection net 1 is a 200-mesh stainless steel filter, and the egg grain collection net 2 is a 500-mesh stainless steel filter. Actual experiments have shown that when both the egg sac collection net 1 and the egg grain collection net 2 are 500-mesh stainless steel filters, good separation of egg sacs, eggs, and larvae can be achieved, resulting in good experimental results. Both the water container 6 and the nematode collection funnel 3 are made of corrosion-resistant transparent glass, allowing for disinfection and reuse multiple times, and facilitating observation of the internal water level and the condition of egg sacs, eggs, and larvae.

[0021] This separation device consists of two parts: the upper part includes a first-layer egg sac collection net 1 for holding root-knot nematode egg sacs, a second-layer high-density egg collection net 2 for holding root-knot nematode eggs, and a water tank 6. The lower part includes a nematode collection funnel 3, a rubber tube 4 for intercepting nematode suspension, and a water-stopping clamp 5.

[0022] The specific operating method of this separation device is as follows:

[0023] (1) Nematode egg sac collection: The experimenters collected plant root samples infected with root-knot nematodes from the field, picked out the egg sacs with a dissecting needle under a stereomicroscope, and injected them into the egg sac collection net 1 of this separation device after suction pipette, in preparation for the next step of disinfection.

[0024] (2) Oocyst disinfection procedure: Close the water-stop clamp 5 at the bottom of the separation device. Pour 75% alcohol or other disinfectant into the water tank 6 in the separation device through the top opening until the liquid level is 1 cm above the surface of the oocysts in the oocyst collection net 1. Gently shake for 30 seconds, then open the bottom water-stop clamp 5 and drain the disinfectant. Pour sterile water into the water tank 6 through the top opening and rinse continuously for 10 seconds. Then close the water-stop clamp 5 and pour sterile water into the water tank 6 until it is 1 cm above the nematode oocysts. This completes the initial disinfection of the oocysts.

[0025] (3) Egg Collection Steps: Gently pry open the sterilized oocysts from the previous steps with a dissecting needle to scatter the eggs. Gently vibrate the separation device, and the eggs will fall onto the egg collection net 2. After completing this step, open the lower stop clamp 5 and drain the water. Then close the stop clamp 5 again and inject 75% alcohol or other disinfectant into the water tank 6 of the separation device through the upper opening until the liquid level exceeds the oocyst collection net 1. Gently shake for 30 seconds, then open the lower stop clamp 5 and drain the disinfectant. Then inject sterile water through the upper opening and rinse continuously for 10 seconds. Close the stop clamp 5 and pour sterile water into the water tank 6 until it covers the nematode oocysts by 1 cm, thus completing the thorough disinfection of the oocysts and eggs.

[0026] (4) Nematode collection: After thorough disinfection in the above steps, place the entire separation device in a dark environment and wait for a large number of second-instar nematode larvae to hatch. Under the action of gravity, the nematode larvae gradually gather at the bottom of the nematode collection funnel 3. After 1 day, gently open the lower water stop clamp 5. The nematode suspension is released through the intercepting rubber hose 4, and 10-20 ml of the nematode suspension at the bottom is released into the nematode culture dish. A large number of second-instar nematode larvae of the same age can be obtained. They are clean and sterile and can be used for various research experiments.

[0027] The above description is only a preferred embodiment of the present utility model. However, the present utility model should not be limited to the content disclosed in the embodiment and the accompanying drawings. Therefore, any equivalent or modified embodiments made without departing from the spirit disclosed in the present utility model shall fall within the protection scope of the present utility model.

Claims

1. A device for rapid disinfection and separation of juveniles from root-knot nematode egg masses, characterized in that It comprises: a water bucket, the bottom of which is provided with a nematode collecting funnel, the bottom of which is provided with a rubber soft hose, the bottom of which is provided with a water stop clamp, and the inside of which is provided with egg capsule collecting nets and egg granule collecting nets distributed in parallel and spaced apart.

2. The device for rapid disinfection and separation of juveniles of root-knot nematode egg mass according to claim 1, characterized in that: The density of the egg capsule collecting nets is less than that of the egg granule collecting nets.

3. The device for rapid disinfection and hatching of root-knot nematode eggs according to claim 1 or 2, wherein: The egg capsule collecting nets are 200-mesh stainless steel filter nets, and the egg granule collecting nets are 500-mesh stainless steel filter nets.

4. The device for rapid disinfection and hatching of root-knot nematode eggs of claim 1, wherein: The material of the water bucket is corrosion-resistant transparent glass or transparent plastic.

5. The device for rapid disinfection and hatching of root-knot nematode eggs of claim 1, wherein: The material of the nematode collecting funnel is corrosion-resistant transparent glass or transparent plastic.

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