Plant extract with redness-reducing effect, its manufacturing method and application

Abstract

A plant extract having a redness-reducing effect and its manufacturing method and application, the plant extract having a redness-reducing effect, comprising, by mass, 1.5 to 6 parts of a root extract of Sophora Root, 1 to 4 parts of a fruit extract of Tribulus Terrestris, and 0.5 to 2 parts of a fruit extract of Cornus Cherry, and Ca 2+ By solving the problem of skin redness from multiple angles, such as inhibiting channel activation, inhibiting vasodilator factor (PGE-2), and inhibiting inflammatory mediators, it is possible to achieve rapid redness removal effects.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application claims priority to a Chinese patent application bearing application number 202310125558.1 and entitled "Plant extract with redness-reducing effect and its manufacturing method and application" filed with the China Patent Office on February 14, 2023, the entire contents of which are incorporated herein by reference. [Technical Field]

[0002] The present invention relates to the technical field of skin care products, and in particular to a plant extract having redness-reducing properties, and its preparation method and application. [Background technology]

[0003] Sensitive skin is a state of high reactivity that occurs in the skin under physiological or pathological conditions, usually occurring on the face, and the triggering factors may be physical, chemical or neuropsychiatric. Sensitive skin is commonly described as itching, burning, redness, tightness, and scaling. Research has found that the most troublesome skin symptom for patients with severe sensitivity is facial redness. Therefore, there is an urgent need for cosmetic ingredients that can resolve facial redness with "fast and long-lasting effects" and have clear traceability. However, there are not many plant extracts in the cosmetic field that can effectively remove redness, and the one that is commonly used is bisabolol, which is an oil-soluble ingredient and has anti-inflammatory properties. Moreover, the action pathway of a single plant extract is relatively narrow, which is disadvantageous for solving skin problems comprehensively and quickly. Summary of the Invention

[0004] The object of the present invention is to provide a plant extract having a redness-reducing effect that can solve the problem of redness on the skin from multiple angles.

[0005] Another object of the present invention is to provide a method for producing a plant extract having redness-reducing properties.

[0006] Yet another object of the present invention is to provide an application of plant extracts having redness-reducing activity in the manufacture of skin care products.

[0007] In order to achieve the above object of the present invention, the present invention provides a plant extract having a redness-removing effect, which comprises, by mass, 1.5 to 6 parts of Sophora Radix root extract, 1 to 4 parts of Tribulus Terrestris fruit extract, and 0.5 to 2 parts of Cornus Cherry fruit extract.

[0008] The plant extracts in an embodiment of the present invention comprise, by mass, 3 parts of Sophora Radix root extract, 2 parts of Tribulus Terrestris fruit extract, and 1 part of Cornus Cherry fruit extract.

[0009] The plant extract according to an embodiment of the present invention contains, by mass, 85 to 95 parts of butanediol and 2 to 6 parts of water. Furthermore, the plant extract contains, by mass, 90 parts of butanediol and 4 parts of water.

[0010] In an embodiment of the present invention, the Sophora Root extract is an aqueous ethanol extract of Sophora Root root, the Tribulus Terrestris fruit extract is an aqueous ethanol extract of Tribulus Terrestris fruit, and the Cornus Cherry fruit extract is an aqueous ethanol extract of Cornus Cherry fruit. Furthermore, the volume fraction of ethanol in the ethanol aqueous solution is 90% to 95%.

[0011] In another aspect, the present invention provides a method for producing a plant extract having a redness-reducing effect, comprising: The method involves extracting the roots of Sophora Root, fruit of Tribulus Terrestris and fruit of Cornus Cherry using an aqueous ethanol solution as the extraction solvent at a ratio of raw material to solvent of 1:10 to 1:30 at 65 to 85°C for 1 to 3 hours.

[0012] In an embodiment of the present invention, the method further comprises the step of fumigating the root of Sophora Root, fruit of Tribulus Terrestris and fruit of Cornus Cherry before the extraction.

[0013] In an embodiment of the present invention, the method further comprises concentrating the extract obtained by the extraction and redissolving it in 1,3-butanediol.

[0014] In an embodiment of the present invention, the redissolution includes adding 1,3-butanediol in multiple portions, and performing a concentration treatment after each addition. Furthermore, the 1,3-butanediol is added in at least two portions, for example, in three to five portions.

[0015] In an embodiment of the present invention, the volume fraction of ethanol in the aqueous ethanol solution is 90% to 95%.

[0016] In an embodiment of the present invention, the ratio of the raw material to the solvent is 1:15 to 1:20.

[0017] In an embodiment of the present invention, the extraction temperature is 75 to 85°C.

[0018] In an embodiment of the present invention, the extraction time is 2 to 3 hours.

[0019] In an embodiment of the present invention, the mass ratio of the Sophora Root root, the Tribulus Terrestris fruit, and the Cornus Cherry fruit is (15-60):(10-40):(5-20), for example, 3:2:1.

[0020] In the reconstitution process according to an embodiment of the present invention, the amount of 1,3-butanediol used is 10 to 25 times the weight of the herbal medicine, based on the amount of the herbal medicine. Furthermore, the amount of 1,3-butanediol used is 10 to 16 times the weight of the crude drug.

[0021] In a further aspect, the present invention provides the use of any of the above-mentioned plant extracts having a redness-reducing activity in the manufacture of skin care products.

[0022] In an embodiment of the present invention, the skin care product comprises a skin care product for sensitive skin.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) The present invention provides a plant extract containing a root extract of Sophora Root, a fruit extract of Tribulus Terrestris, and a fruit extract of Cornus Cherry, and the plant extract contains Ca. 2+ By solving the problem of skin redness from multiple angles, such as inhibiting channel activation, inhibiting vasodilator factors, and inhibiting inflammatory mediators, it is possible to achieve rapid redness removal effects. (2) The method for producing the plant extract of the present invention can increase the content of the active ingredient and reduce the amount of residual ethanol, thereby significantly improving the effect of removing redness. [Brief explanation of the drawings]

[0024] In order to more clearly describe the embodiments of the present invention or the solutions in the prior art, the drawings in the following description are embodiments of the present invention, and those skilled in the art can further derive other drawings based on these drawings without any creative work. [Figure 1] 1 shows the test results of the stimulating effect of the plant extracts of Examples 1 and 33 of the present invention on chicken embryos, where the left figure corresponds to the chicken embryos after the action of the plant extract of Example 33, and the right figure corresponds to the chicken embryos after the action of the plant extract of Example 1. [Figure 2] 1 shows the test results of the average skin sensitization TiVi-index in the redness-reducing effect test of the plant extract jelly sample provided in Example 1 of the present invention. [Figure 3] 1 shows the test results of the average rate of change in skin sensitization TiVi-index from the initial value in the redness-reducing effect test of the plant extract jelly sample provided in Example 1 of the present invention. [Figure 4] 1 shows TiVi pictures of the plant extract jelly sample provided in Example 1 of the present invention before and after use at different times. [Figure 5]1 shows the test results of skin color a* values ​​in a test of the redness-reducing effect of plant extract jelly samples provided in Examples 23 to 26 and 33 of the present invention and Comparative Examples 1 and 2. [Figure 6] This shows the test results of the average rate of change in skin sensitization TiVi-index from the initial value in a test of the redness removal effect of plant extract jelly samples provided in Examples 23 to 26, Example 33, and Comparative Examples 1 and 2 of the present invention. [Figure 7] FIG. 1 is a graph showing the calcium flux test results of the plant extract provided in Example 1 of the present invention. [Figure 8] FIG. 1 is a graph showing the results of a test to test the plant extract provided in Example 1 of the present invention against the UV-induced inflammatory factor TNF-α. [Figure 9] FIG. 1 is a graph showing the results of a test to test the suppression of LPS-induced IL-8 by the plant extract provided in Example 1 of the present invention. [Figure 10] FIG. 1 shows the results of a test to test the inhibition of UV-induced PGE2 by the plant extracts provided in Example 1 of the present invention. [Figure 11] FIG. 1 is a graph showing the results of an inhibitory test of UV-induced COX-2 by the plant extract provided in Example 1 of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0025] The following describes the solution of the present invention with reference to the drawings and embodiments. However, the examples described below are only some of the examples of the present invention, not all of the examples, and are used only to explain the present invention and do not limit the scope of the present invention. Any other embodiments based on the embodiments of the present invention fall within the scope of protection of the present invention. Those who do not specify specific conditions in the examples follow the usual conditions or the conditions suggested by the manufacturer. Reagents and equipment used without a manufacturer's name were all commercially available standard products.

[0026] In one aspect of the present invention, a plant extract having a redness-removing effect is provided, which comprises, by mass, 1.5 to 6 parts of Sophora Root Extract, 1 to 4 parts of Tribulus Terrestris Fruit Extract, and 0.5 to 2 parts of Cornus Cherry Fruit Extract.

[0027] For example, the parts by mass of each component in the plant extract having the redness-reducing effect can be exemplified as follows: The sour herb root extract may be 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, etc. The Tribulus terrestris fruit extract may be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, etc. Cornus cherry fruit extract may be 0.5 parts, 0.8 parts, 1 part, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, and the like.

[0028] The amounts of the Sophora Root Extract, Tribulus Terrestris Fruit Extract, and Cornus Cherry Fruit Extract used in the plant extracts of the present invention are calculated based on the amounts of the raw materials used, namely, Sophora Root, Tribulus Terrestris Fruit, and Cornus Cherry Fruit, respectively.

[0029] Sophora root is the root of the legume Sophora Root, which contains various alkaloids such as matrine, oxymatrine, sophoranol, and sophorine, and has various pharmacological effects such as antibacterial, anti-inflammatory, anti-allergic, and immunomodulatory. It is mainly used to treat skin diseases caused by wind-heat and damp-heat, such as eczema and pruritus. Tribulus terrestris has a bitter and spicy taste, a warm nature, and has the effects of calming the liver, relieving depression, brightening the eyes, and relieving itching. The chemical components of Tribulus terrestris mainly include saponins, flavones, alkaloids, etc., of which saponins are the main active ingredients. Modern pharmacological research has discovered that the saponin components of Tribulus terrestris have multiple effects, including anti-atherosclerosis, antioxidant properties, and improved cardiac function. The main chemical components of Cornus officinalis include iridoids and their glycosides, triterpenes, flavones (flavonoids), tannins, and organic acids, which have various physiological activities such as neuroprotection, anti-inflammatory, hypoglycemic, antibacterial, antitumor, and hepatoprotection. The present invention uses and combines a certain amount of Sophora Root Extract, Tribulus Terrestris Fruit Extract, and Cornus Cherry Fruit Extract to achieve the following: 2+ By solving the problem of skin redness from multiple angles, such as inhibiting channel activation, inhibiting vasodilator factors, and inhibiting inflammatory mediators, it is possible to achieve rapid redness removal effects.

[0030] The plant extracts in this embodiment contain, by mass, 3 parts of Sophora Radix root extract, 2 parts of Tribulus Terrestris fruit extract, and 1 part of Cornus Cherry fruit extract.

[0031] Furthermore, the plant extract of this embodiment contains, in parts by mass, 85 to 95 parts of 1,3-butanediol and 2 to 6 parts of water. Furthermore, the plant extract contains, by mass, 90 parts of 1,3-butanediol and 4 parts of water.

[0032] For example, the parts by mass of 1,3-butanediol and water in the plant extract can be exemplified as follows: The 1,3-butanediol may be 85 parts, 86 parts, 87 parts, 88 parts, 89 parts, 90 parts, 91 parts, 92 parts, 93 parts, 94 parts, 95 parts, etc., and the water may be 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, etc.

[0033] In this embodiment, the Sophora Root extract is an aqueous ethanol extract of Sophora Root root, the Tribulus Terrestris fruit extract is an aqueous ethanol extract of Tribulus Terrestris fruit, and the Cornus Cherry fruit extract is an aqueous ethanol extract of Cornus Cherry fruit. Furthermore, the volume fraction of ethanol in the ethanol aqueous solution is 90% to 95%.

[0034] The volume fraction of ethanol in the aqueous ethanol solution may be 90%, 91%, 92%, 93%, 94%, 95%, or the like.

[0035] The plant extract in this embodiment contains flavone and oxymatrine in a mass ratio of (1.3 to 2.0):1, for example, (1.55 to 1.6):1.

[0036] For example, the mass ratio of flavone to oxymatrine in the plant extract may be 1.3:1, 1.35:1, 1.4:1, 1.45:1, 1.5:1, 1.55:1, 1.6:1, 1.65:1, 1.7:1, 1.75:1, 1.8:1, 1.85:1, 1.9:1, 2.0:1, etc.

[0037] In another aspect, the present invention provides a method for producing a plant extract having a redness-removing effect, which comprises the steps of extracting Sophora Root, Tribulus Terrestris Fruit, and Cornus Cherry Fruit using an aqueous ethanol solution as an extraction solvent at a raw material to solvent ratio of 1:10 to 1:30 at 65 to 85°C for 1 to 3 hours.

[0038] For example, the ratio of the raw material to the solvent may be 1:10 (m / m), 1:12 (m / m), 1:15 (m / m), 1:18 (m / m), 1:20 (m / m), 1:22 (m / m), 1:25 (m / m), 1:28 (m / m), 1:30 (m / m), etc. Here, the ratio of raw material to solvent refers to the ratio of dried plant material that has not been subjected to pretreatment (e.g., fumigation) to extraction solvent.

[0039] For example, the extraction temperature may be 65°C, 70°C, 75°C, 80°C, 85°C, etc., and the extraction time may be 1 hour, 1.5 hours, 2 hours, 2.5 hours, 3 hours, etc.

[0040] Furthermore, this embodiment includes a step of subjecting the root of Sophora Root, fruit of Tribulus Terrestris, and fruit of Cornus Cherry to a fumigation treatment before the extraction. Furthermore, the duration of the fumigation treatment is ≧20 minutes, for example, 20 to 40 minutes.

[0041] For example, the fumigation treatment time may be 20 minutes, 25 minutes, 30 minutes, 35 minutes, 40 minutes, etc. Here, the fumigation refers to fumigation using water vapor.

[0042] Furthermore, this embodiment includes a step of concentrating the extract obtained by the extraction and redissolving it in 1,3-butanediol.

[0043] In actual operation, the extracted material is cooled to room temperature and filtered through gauze to obtain the extract. The filtering method can be adjusted according to the actual situation.

[0044] In this embodiment, the redissolution includes adding 1,3-butanediol in multiple portions, and performing a concentration treatment after each addition. Furthermore, the 1,3-butanediol is added in at least two portions, for example, in three to five portions.

[0045] For example, 1,3-butanediol in the redissolution may be added in two, three, four, or five separate portions.

[0046] In actual operation, the concentration method can be selected based on the usual operation, for example, negative pressure concentration.

[0047] In the redissolution in this embodiment, the amount of 1,3-butanediol added in a single step is 15% to 25% of the total amount of 1,3-butanediol.

[0048] For example, the amount of 1,3-butanediol added in a single step during the redissolution may be 15%, 18%, 20%, 22%, 25%, etc., of the total amount of 1,3-butanediol.

[0049] The volume fraction of ethanol in the aqueous ethanol solution in this embodiment is 90% to 95%.

[0050] For example, the volume fraction of ethanol in the aqueous ethanol solution may be 90%, 91%, 92%, 93%, 94%, 95%, or the like.

[0051] By using the above-mentioned extraction solvent, it is possible to effectively extract the active ingredients and obtain a plant extract having the effect of removing redness.

[0052] In this embodiment, the ratio of raw material to solvent is 1:15 to 1:20, and the ratio of raw material to solvent is 1:20.

[0053] The extraction temperature in this embodiment is 75 to 85°C, for example, the extraction temperature is 75°C.

[0054] In this embodiment, the extraction time is 2 to 3 hours, for example, the extraction time is 2 hours.

[0055] In this embodiment, the mass ratio of the root of Sophora Root, the fruit of Tribulus Terrestris, and the fruit of Cornus Cherry is (15-60):(10-40):(5-20), for example, 3:2:1.

[0056] For example, the mass ratio of the Sophora Root, Tribulus Terrestris Fruit and Cornus Cherry Fruit may be 3:2:1, 3:3:1, 1:1:1, 2:3:1, 2:3:2, 2:2:1, 3:3:2, etc.

[0057] In the reconstitution in this embodiment, the amount of 1,3-butanediol used is 10 to 25 times the weight of the herbal medicine, based on the amount of the herbal medicine. Furthermore, the amount of 1,3-butanediol used is 10 to 16 times the weight of the crude drug.

[0058] For example, in the reconstitution, the amount of 1,3-butanediol used may be 10 times, 11 times, 12 times, 13 times, 14 times, 15 times, 16 times, 17 times, 18 times, 19 times, 20 times, 21 times, 22 times, 23 times, 24 times, 25 times, etc., based on the amount of the herbal medicine, and is preferably 16 times.

[0059] In a further aspect, the present invention provides the use of any of the above-mentioned plant extracts having redness-reducing activity in the manufacture of skin care products.

[0060] In this embodiment, the skin care products include skin care products for sensitive skin.

[0061] For example, Ca 2+ The application of the plant extract having redness-reducing activity in the manufacture of skin care products for inhibiting channel activation, inhibiting vasodilator factors and / or inhibiting the expression of inflammatory mediators.

[0062] The plant extract of the present invention can comprehensively and quickly solve the problem of skin redness through multi-pathway and multi-target.

[0063] In the following examples and comparative examples, unless otherwise specified, the raw materials used are coarse powder of the root of Sophora Root, fruit of Tribulus Terrestris, and fruit of Cornus Cherry, which are passed through a 6-mesh sieve and collected.

[0064] (Examples 1 to 7) Examples 1 to 7 provide a method for producing a plant extract having a redness-reducing effect, Step (1) of weighing the root of bitter melon, the fruit of Tribulus terrestris, and the fruit of Cornus japonica in a certain ratio, adding water to a steamer and boiling it, and then placing the root of bitter melon, the fruit of Tribulus terrestris, and the fruit of Cornus japonica in the steamer and fumigating them for 20 minutes; Step (2) adding an aqueous solution of ethanol with a volume fraction of 95% to the fumigated material in a raw material to solvent ratio of 1:20 and extracting at 75 ° C for 2 hours; Step (3) cooling the extracted material to room temperature and filtering it through gauze to obtain an extract, and then placing the extract in a flask of a rotary evaporator and concentrating it under reduced pressure until the liquid adheres to the wall of the flask to obtain a concentrated liquid; Step (4) is added to the concentrate obtained in step (3) in 16 times the amount of herbal medicine to redissolve it, and in this redissolution, 1,3-butanediol is added in 5 portions, each time adding 20% ​​of the total amount of 1,3-butanediol, and after each addition, the material is transferred to a rotary evaporator flask and concentrated under reduced pressure until the mass is constant; and step (5) of cooling the concentrate obtained in step (4) to below 35°C, filtering it through an H70 filter paper plate, sterilizing the filtrate at 80-85°C for 40 minutes, and mixing it uniformly after cooling to obtain a plant extract having redness-removing properties.

[0065] Table 1 shows the mass ratios of the root of Sophora Root, fruit of Tribulus Terrestris, and fruit of Cornus Cherry specifically used in the manufacturing methods of Examples 1 to 7, and the flavone content (standardized as rutin) in the obtained plant extracts.

[0066] [Table 1]

[0067] (Examples 8 to 9) Examples 8 and 9 provide a method for producing a plant extract having a redness-reducing effect. Step (1) of weighing the root of bitter melon, the fruit of Tribulus terrestris, and the fruit of Cornus japonica in a mass ratio of 3:2:1, adding water to a steamer and boiling it, and then placing the root of bitter melon, the fruit of Tribulus terrestris, and the fruit of Cornus japonica in the steamer and fumigating them for 20 minutes; Step (2) of adding an aqueous ethanol solution having a volume fraction of 95% to the fumigated material at a constant raw material to solvent ratio and extracting at 75 ° C for 2 hours; Step (3) cooling the extracted material to room temperature and filtering it through gauze to obtain an extract, and then placing the extract in a flask of a rotary evaporator and concentrating it under reduced pressure until the liquid adheres to the wall of the flask to obtain a concentrated liquid; Step (4) is added to the concentrate obtained in step (3) in a 16-fold amount of 1,3-butanediol to redissolve the herb, and in this redissolution, 1,3-butanediol is added in 5 portions, each time adding 20% ​​of the total amount of 1,3-butanediol, and after each addition, the material is transferred to a rotary evaporator flask and concentrated under reduced pressure until the mass is constant; and step (5) of cooling the concentrate obtained in step (4) to below 35°C, filtering it through an H70 filter paper plate, sterilizing the filtrate at 80-85°C for 40 minutes, and mixing it uniformly after cooling to obtain a plant extract having redness-removing properties.

[0068] The ratios of raw materials and solvents specifically employed in the manufacturing methods of Examples 1, 8 and 9, and the flavone contents (rutin standard) in the obtained plant extracts are shown in Table 2.

[0069] [Table 2]

[0070] (Examples 10 to 13) Examples 10 to 13 provide a method for producing a plant extract having a redness-reducing effect, Step (1) of weighing the root of bitter melon, the fruit of Tribulus terrestris, and the fruit of Cornus japonica in a mass ratio of 3:2:1, adding water to a steamer and boiling it, and then placing the root of bitter melon, the fruit of Tribulus terrestris, and the fruit of Cornus japonica in the steamer and fumigating them for 20 minutes; Step (2) adding a 95% volumetric ethanol aqueous solution to the fumigated material in a raw material to solvent ratio of 1:20 and extracting at 75 ° C for 1 to 3 hours; Step (3) cooling the extracted material to room temperature and filtering it through gauze to obtain an extract, and then placing the extract in a flask of a rotary evaporator and concentrating it under reduced pressure until the liquid adheres to the wall of the flask to obtain a concentrated liquid; Step (4) is added to the concentrate obtained in step (3) in 16 times the amount of herbal medicine to redissolve it, and in this redissolution, 1,3-butanediol is added in 5 portions, each time adding 20% ​​of the total amount of 1,3-butanediol, and after each addition, the material is transferred to a rotary evaporator flask and concentrated under reduced pressure until the mass is constant; and step (5) of cooling the concentrate obtained in step (4) to below 35°C, filtering it through an H70 filter paper plate, sterilizing the filtrate at 80-85°C for 40 minutes, and mixing it uniformly after cooling to obtain a plant extract having redness-removing properties.

[0071] Table 3 shows the extraction times specifically employed in the production methods of Examples 1 and 10 to 13, and the flavone contents (rutin standard) in the obtained plant extracts.

[0072] [Table 3]

[0073] (Examples 14 to 17) Examples 14 to 17 provide a method for producing a plant extract having a redness-reducing effect, Step (1) of weighing the root of bitter melon, the fruit of Tribulus terrestris, and the fruit of Cornus japonica in a mass ratio of 3:2:1, adding water to a steamer and boiling it, and then placing the root of bitter melon, the fruit of Tribulus terrestris, and the fruit of Cornus japonica in the steamer and fumigating them for 20 minutes; Step (2) of adding an aqueous ethanol solution having a volume fraction of 95% to the fumigated material at a raw material to solvent ratio of 1:20 and extracting at 65 to 85 ° C for 2 hours; Step (3) cooling the extracted material to room temperature and filtering it through gauze to obtain an extract, and then placing the extract in a flask of a rotary evaporator and concentrating it under reduced pressure until the liquid adheres to the wall of the flask to obtain a concentrated liquid; Step (4) is added to the concentrate obtained in step (3) in 16 times the amount of herbal medicine to redissolve it, and in this redissolution, 1,3-butanediol is added in 5 portions, each time adding 20% ​​of the total amount of 1,3-butanediol, and after each addition, the material is transferred to a rotary evaporator flask and concentrated under reduced pressure until the mass is constant; and step (5) of cooling the concentrate obtained in step (4) to below 35°C, filtering it through an H70 filter paper plate, sterilizing the filtrate at 80-85°C for 40 minutes, and mixing it uniformly after cooling to obtain a plant extract having redness-removing properties.

[0074] Table 4 shows the extraction temperatures specifically employed in the production methods of Examples 1 and 14 to 17, and the flavone contents (rutin standard) in the obtained plant extracts.

[0075] [Table 4]

[0076] (Examples 18 to 22) Examples 18 to 22 provide methods for producing plant extracts having redness-reducing properties, Step (1) of weighing the root of bitter melon, the fruit of Tribulus terrestris, and the fruit of Cornus japonica in a mass ratio of 3:2:1, adding water to a steamer and boiling it, and then placing the root of bitter melon, the fruit of Tribulus terrestris, and the fruit of Cornus japonica in the steamer and fumigating them for 20 minutes; Step (2) adding an aqueous solution of ethanol with a volume fraction of 95% to the fumigated material in a raw material to solvent ratio of 1:20 and extracting at 75 ° C for 2 hours; Step (3) cooling the extracted material to room temperature and filtering it through gauze to obtain an extract, and then placing the extract in a flask of a rotary evaporator and concentrating it under reduced pressure until the liquid adheres to the wall of the flask to obtain a concentrated liquid; Step (4) is added to the concentrate obtained in step (3) by adding 1,3-butanediol in a certain multiple of the amount of herbal medicine to redissolve it, and in this redissolution, 1,3-butanediol is added in 5 portions, and 20% of the total amount of 1,3-butanediol is added each time, and after each addition, the material is transferred to a rotary evaporator flask and concentrated under reduced pressure until the mass is constant; and step (5) of cooling the concentrate obtained in step (4) to below 35°C, filtering it through an H70 filter paper plate, sterilizing the filtrate at 80-85°C for 40 minutes, and mixing it uniformly after cooling to obtain a plant extract having redness-removing properties.

[0077] Table 5 shows the amount of 1,3-butanediol used specifically in the production methods of Examples 1 and 18 to 22, and the flavone content (standardized as rutin) in the resulting plant extracts.

[0078] [Table 5]

[0079] Example 23 This example is based on the manufacturing method of Example 1, and the only difference is that this example does not include the fumigation treatment in step (1) of Example 1.

[0080] Example 24 This example is based on the manufacturing method of Example 1, with the difference being that this example does not include the fumigation treatment in step (1) of Example 1, and that this example uses an equal weight of propylene glycol instead of the redissolving solvent 1,3-butanediol in Example 1.

[0081] Example 25 This example is based on the manufacturing method of Example 1, except that this example does not include the fumigation treatment in step (1) of Example 1, and instead places the material in a high-pressure sterilizer and treats it at low pressure (0.1 MPa) for 20 minutes.

[0082] Example 26 This example is based on the manufacturing method of Example 25, and differs only in step (3). In step (3) of this example, the extracted material is cooled to room temperature, filtered through gauze to obtain an extract, and 2 wt% (based on the mass of the extract) of activated carbon is added and deodorized at 60°C for 1 hour. The extract is then filtered through an F100 filter paper plate to collect the filtrate, which is then placed in a rotary evaporator flask and concentrated under reduced pressure until the liquid adheres to the wall of the flask, thereby obtaining a concentrated liquid.

[0083] The color, appearance, odor and corresponding physicochemical indices of the products obtained in Example 1, Example 24 to Example 26 were recorded and tested. The results are shown in Table 6.

[0084] [Table 6]

[0085] (Examples 27 to 30) This example provides a method for producing a plant extract having redness-reducing properties, Step (1) of weighing the root of Sophora Root, the fruit of Tribulus Terrestris, and the fruit of Cornus Cherry in a mass ratio of 3:2:1, adding water to a steamer and boiling it, and then placing the root of Sophora Root, the fruit of Tribulus Terrestris, and the fruit of Cornus Cherry in the steamer and fumigating them for a certain period of time; Step (2) adding an aqueous solution of ethanol with a volume fraction of 95% to the fumigated material in a raw material to solvent ratio of 1:20 and extracting at 75 ° C for 2 hours; Step (3) cooling the extracted material to room temperature and filtering it through gauze to obtain an extract, and then placing the extract in a flask of a rotary evaporator and concentrating it under reduced pressure until the liquid adheres to the wall of the flask to obtain a concentrated liquid; Step (4) is added to the concentrate obtained in step (3) in 16 times the amount of herbal medicine to redissolve it, and in this redissolution, 1,3-butanediol is added in 5 portions, each time adding 20% ​​of the total amount of 1,3-butanediol, and after each addition, the material is transferred to a rotary evaporator flask and concentrated under reduced pressure until the mass is constant; and step (5) of cooling the concentrate obtained in step (4) to below 35°C, filtering it through an H70 filter paper plate, sterilizing the filtrate at 80-85°C for 40 minutes, and mixing it uniformly after cooling to obtain a plant extract having redness-removing properties.

[0086] Table 7 shows the fumigation times specifically employed in the manufacturing methods of Examples 1, 23, and 27 to 30, and the oxymatrine contents in the obtained plant extracts.

[0087] [Table 7]

[0088] Example 31 This embodiment is based on the manufacturing method of the first embodiment, and differs only in step (4). In step (4) of this example, 1,3-butanediol is added to the concentrate obtained in step (3) in an amount 16 times the amount of herbal medicine to redissolve it. During the redissolution, 1,3-butanediol is added in two portions, 70% in the first portion and 30% in the second portion. After each addition, the material is transferred to a rotary evaporator flask and concentrated under reduced pressure until the mass remains constant.

[0089] Example 32 This embodiment is based on the manufacturing method of the first embodiment, and differs only in step (4). In step (4) of this example, 1,3-butanediol is added to the concentrate obtained in step (3) in an amount 16 times the amount of herbal medicine to redissolve it. During the redissolution, 1,3-butanediol is added in two portions, 50% in the first addition and 50% in the second addition. After each addition, the material is transferred to a rotary evaporator flask and concentrated under reduced pressure until the mass remains constant.

[0090] Example 33 This embodiment is based on the manufacturing method of the first embodiment, and differs only in step (4). In step (4) of this example, 1,3-butanediol was added to the concentrate obtained in step (3) in an amount 16 times the amount of the herb to redissolve it, and the material was then transferred to a rotary evaporator flask and concentrated under reduced pressure until the mass was constant.

[0091] Table 8 shows the ethanol contents in the plant extracts specifically obtained by the production methods of Examples 1 and 31 to 33. Here, the test method for ethanol content refers to the gas chromatography-mass spectrometry method in Chapter 4, 2.33 of the "Cosmetics Safety Technical Standards" (2015 edition).

[0092] [Table 8]

[0093] The stimulating effects of the samples of Example 1 and Example 33 on chicken embryos were tested, and the test results are shown in FIG. As can be seen from the figure, the sample of Example 33 had a stimulating effect on the chicken embryos, dissolving the capillaries, whereas the sample of Example 1 did not show any stimulating effect on the chicken embryos.

[0094] The test method includes: 1. Setting of test groups (1) Solvent control group: physiological saline, i.e., 0.9% NaCl aqueous solution. (2) Model control group: 1% SDS, weigh out 0.1 g of SDS and add it to 9.9 g of saline, then vortex mix. (3) Negative control group: Obtained by diluting 0.1% SDS or 1% SDS 10 times. (4) Sample group: Dilute the sample to 20% with saline, take 2 mL, add 2 mL of 0.2% SDS, vortex mix, and obtain a final concentration of 10%. 2. Exam procedure (1) CAM production: Chicken embryos are cultured until they are 9 days old, and egg irradiation tests are performed. The eggshell at the air cell area is peeled off using dental sawtooth curved forceps to expose the white egg membrane, which is carefully manipulated to avoid destroying the integrity of the egg membrane. Wet the eggshell membrane with a few drops of saline using a straw and carefully remove the eggshell membrane with tweezers, ensuring that the eggshell membrane is not damaged. At this time, the vasculature is again inspected and its integrity and suitability for testing is determined. (2) End-point evaluation procedure: 0.3 mL of the test substance was taken and applied directly to the CAM. After 3 minutes of application, the substance was photographed and observed, and the degree of change in vascular toxicity, i.e., the degree of vascular bleeding, blood coagulation, and vascular dissolution, was recorded. Six chicken embryos are used for each test substance, six chicken embryos for the negative control (0.1% SDS), and one chicken embryo for the solvent control.

[0095] (Comparative Example 1) Comparative Example 1 is based on the manufacturing method of Example 1, with the difference being that the raw materials used in step (1) do not include terrestris fruit or Chinese quince fruit, but only include sour root, and no fumigation treatment is performed in step (1).

[0096] (Comparative Example 2) Comparative Example 2 is based on the manufacturing method of Example 1, with the difference being that the raw materials used in step (1) do not include the roots of Sophora Root or the fruit of Cornus Jujube, but only the fruit of Tribulus Terrestris, and no fumigation treatment is performed in step (1).

[0097] The color, appearance, odor and corresponding physicochemical indexes of the products obtained in Comparative Example 1 and Comparative Example 2 were recorded and tested, and the results are shown in Table 9.

[0098] [Table 9]

[0099] (Experimental Example 1) Human Efficacy Testing Test Method: After stimulating the face with capsaicin, polarized light images of the skin of the test area were collected using TiVi 700 and analyzed at 0 min, 1 min, 3 min, 5 min, 7 min, 10 min, 15 min, 20 min, and 30 min after sample application to obtain TiVi images. Polarized images are closer to the actual situation seen with the naked eye, and image analysis by Tivi 700 uses a digital method to distinguish the degree of irritation by color; the more red the color is, the more severe the skin irritation. The test results are shown in Figures 2 to 4. The compositions of the plant extract jelly sample and blank matrix (substrate) of Example 1 are shown in Table 10.

[0100] [Table 10]

[0101] Note: In the plant extract jelly sample, the amount of plant extract used is calculated based on the total amount of liquid plant extract product.

[0102] As can be seen from the above test results, the plant extract jelly sample of the present invention showed significant differences compared to the blank matrix at 0, 10, 15, 20, and 30 minutes after use, indicating that the plant extract of the present invention has a significant redness-reducing effect.

[0103] Furthermore, the redness-removing effects of Examples 23 to 26, Example 33, and Comparative Examples 1 and 2 were tested. The plant extracts of each Example and Comparative Example were prepared into test samples based on the formulation of the plant extract jelly sample in Table 10, and tested according to the above test method. The test results are shown in Figures 5 and 6. The results of the skin color a* value test and the skin sensitization Tivi-index test show that the redness-removing effects of the plant extracts of Examples 23 to 26 and Example 33 are not as good as those of the plant extract of Example 1.

[0104] (Experimental Example 2) Skin redness signaling pathway test 1. Stimulation of keratinocytes by capsaicin (Ca 2+ concentration) Calcium Flux Test Method: (1) Cell inoculation: 1.8×10 5 Keratinocytes were seeded into a 6-well plate at a seeding density of 10 cells / well and incubated overnight in an incubator (37°C, 5% CO2). (2) Liquid preparation: Prepare working solutions of each test substance based on the test grouping. Specific test grouping information is shown in Table 11. The specific solvent used to prepare the samples is PBS buffer, and the concentrations are mass fractions and are calculated based on the total mass of the plant extract of Example 1.

[0105] [Table 11] (3) Probe loading: The cell culture medium was removed by aspiration, and the cells were washed three times with PBS. 0.5 mL of probe loading working solution was added to each well, and the cells were incubated in an incubator at 37°C for 45 minutes. After the incubation was completed, the probe working solution was removed by aspiration, and the plate was washed three times with PBS. (4) Administration: For the BC group, 0.5 mL of PBS was added to each well; for the NC group, a PBS solution containing 0.05 μM capsaicin CAP was added to each well; for the PC group, a PBS solution containing 0.05 μM capsaicin CAP and 3.9 μg / mL trans-4-tert-butylcyclohexanol was added to each well; and for the sample group, 0.5 mL of a PBS solution containing 0.05 μM capsaicin CAP and the working concentration sample was added to each well. After administration, the mice were incubated in an incubator for 1 hour. (5) Calcium flux detection: After the incubation was completed, the cells were observed and photographed using a fluorescence microscope, and the cell morphology was collected under a 200× field of view. The imaging time was controlled to 30 min. (6) Statistical analysis of results: Graphs were created using GraphPad Prism, and the results were expressed as Mean ± SD. Comparisons between groups will be performed using statistical analysis using t-test. All statistical analyses will be two-sided. P<0.05 was judged to indicate a significant difference, and P<0.01 to indicate an extremely significant difference.

[0106] The test results are shown in Figure 7. As can be seen from Figure 7, the plant extract of the present invention has a Ca 2+ The internal inflow of ions can be effectively reduced.

[0107] (Experimental Example 3) Inflammatory mediator inhibition test 1, TNF-α (UVB-keratinocytes) Test method: (1) Cell inoculation: 2.2 x 10 5 Keratinocytes were seeded into a 6-well plate at a seeding density of 10 cells / well and incubated overnight in an incubator (37°C, 5% CO2). (2) Liquid preparation: Prepare working solutions of each test substance based on the test grouping. Specific test grouping information is shown in Table 12.

[0108] [Table 12] (3) Administration: According to the test plan, when the cell plating rate in the 6-well plate reached 40%-60%, the cells were divided into groups and administered. 2 mL of sample was added to each well, and three replicate wells were set up for each group. After administration was completed, the 6-well plate was placed in an incubator (37°C, 5% CO2) and cultured for 24 hours. (4) UVB irradiation: Based on the test group division, the group with UVB irradiation received 300 mJ / cm 2 UVB irradiation was performed. (5) Post-incubation: After washing the cells with PBS, the 6-well plate was placed in an incubator (37°C, 5% CO2) and incubated for 24 hours. (6) ELISA detection: Cell culture supernatant was collected and subjected to detection analysis according to the operating instructions of the ELISA detection kit.

[0109] The test results are shown in Figure 8. As can be seen from FIG. 8, the plant extract of the present invention has the effect of suppressing the expression of TNF-α when the concentrations are 0.313% and 0.625%. 2. IL-8 (chemotactic factor) (LPS-macrophage) Testing method: (1) Cell inoculation: 2.2×10 5 Seed the cells into a 6-well plate at an inoculation density of 1000μL / well, add 2 mL of cell suspension to each well, and place the inoculated cell culture plate into an incubator to continue culturing for 24 h (5% CO, 37 °C). (2) Liquid preparation: Prepare working solutions of each test substance based on the test grouping. Specific test grouping information is shown in Table 13.

[0110] [Table 13] Preparation of working solutions containing 1 μg / mL LPS: The 2 mg / mL LPS stock solution was diluted to 1 μg / mL using the test substance working solution, negative control working solution, and positive control working solution remaining after administration. (3) The old cell culture medium in the wells was removed by suction. To each well of the sample group, 1.8 mL of culture medium containing the corresponding concentration of the test substance was added; to each well of the negative control group, 1.8 mL of solvent control culture medium was added; to each well of the positive control group, 1.8 mL of positive control culture medium was added; and to each well of the blank control group, 1.8 mL of normal culture medium was added. (4) After administration was completed, the 6-well plate was placed in a cell incubator (5% CO2, 37°C) and cultured for 2 hours. (5) LPS stimulation: 2 hours after administration, 200 μL of the prepared working solution containing LPS was added to each well of each group, and the cells were placed in a cell incubator (5% CO , 37°C) and cultured for 22 hours. (6) ELISA detection: Cell culture supernatant was collected and subjected to ELISA detection according to the instructions of the ELISA kit. The test results are shown in Figure 9. As can be seen from FIG. 9, the plant extract of the present invention, when at a concentration of 0.625%, has the effect of suppressing macrophage IL-8 expression, amplifying the inflammatory cascade, and inhibiting angiogenesis. (Experimental Example 4) Inhibition of vasodilator prostaglandin E2 (PGE-2) and cyclooxygenase 2 (COX-2) (UVB-spinocytes) (1) PGE-2 test method (a) Cell inoculation: 2.2×10 5 Keratinocytes were seeded into a 6-well plate at a seeding density of 10 cells / well and incubated overnight in an incubator (37°C, 5% CO2). (b) Solution preparation: Prepare working solutions of each test substance based on the test grouping. Specific test grouping information is shown in Table 14. [Table 14] (c) Administration: According to the test plan, when the cell plating rate in the 6-well plate reached 40%-60%, the cells were divided into groups and administered. 2 mL of sample was added to each well, and three replicate wells were set up for each group. After administration was completed, the 6-well plate was placed in an incubator (37°C, 5% CO2) and cultured for 24 hours. (d) UVB irradiation: Based on the test grouping, the group with UVB irradiation received 300 mJ / cm 2 UVB irradiation was performed. (e) Post-incubation: After washing the cells with PBS, the 6-well plate was placed in an incubator (37°C, 5% CO2) and incubated for 24 hours. (f) ELISA detection: Cell culture supernatant was collected and subjected to detection analysis according to the operating instructions of the ELISA detection kit. The test results are shown in Figure 10. As can be seen from Figure 10, the plant extract of the present invention effectively reduces the release of PGE2 when the concentrations are 0.156%, 0.313% and 0.625%. (2) COX-2 test method (a) Cell inoculation: 2.2×10 5 Keratinocytes were seeded into a 6-well plate at a seeding density of 10 cells / well and incubated overnight in an incubator (37°C, 5% CO2). (b) Solution preparation: Prepare working solutions of each test substance based on the test grouping. Specific test grouping information is shown in Table 15. [Table 15] (c) Administration: According to the test plan, when the cell plating rate in the 6-well plate reached 40%-60%, the cells were divided into groups and administered. 2 mL of sample was added to each well, and three replicate wells were set up for each group. After administration was completed, the 6-well plate was placed in an incubator (37°C, 5% CO2) and cultured for 24 hours. (d) UVB irradiation: Based on the test grouping, the group with UVB irradiation received 300 mJ / cm2 UVB irradiation was performed. (e) Post-incubation: After washing the cells with PBS, the 6-well plate was placed in an incubator (37°C, 5% CO2) and incubated for 24 hours. (f) Detection of gene expression: After culturing for 24 hours, the cells were washed twice with 1 mL / well of PBS, 1 mL of RNAiso Plus was added to each well, the cells were lysed by pipetting, and the samples were then placed in the wells. RNA was extracted and reverse transcribed into cDNA, followed by fluorescent quantitative PCR detection, and the results were calculated using the 2-ΔΔCT method. The test results are shown in Figure 11. As can be seen from Figure 11, the plant extract of the present invention effectively reduces the release of COX-2 at a concentration of 0.625%.

[0111] As can be seen from the above test results, the plant extract of the present invention has the following properties: 2+ By solving the problem of skin redness from multiple angles, such as inhibiting channel activation, inhibiting vasodilator factors, and inhibiting inflammatory mediators, it is possible to achieve rapid redness removal effects. [Industrial Applicability]

[0112] As described above, the present invention provides a plant extract having a redness-reducing effect, as well as a method for producing the same and applications thereof. The plant extracts having a redness-removing effect contain, by mass, 1.5 to 6 parts of Sophora Root Extract, 1 to 4 parts of Tribulus Terrestris Fruit Extract, and 0.5 to 2 parts of Cornus Cherry Fruit Extract. The present invention provides a plant extract containing a root extract of Sophora Root, a fruit extract of Tribulus Terrestris, and a fruit extract of Cornus Cherry, and the plant extract contains Ca 2+ By solving the problem of skin redness from multiple angles, such as inhibiting channel activation, inhibiting vasodilator factor (PGE-2), and inhibiting inflammatory mediators, it is possible to achieve rapid redness removal effects.

Claims

1. A plant extract having a redness-reducing effect, A plant extract having a redness-removing effect, characterized by comprising, by mass, 1.5 to 6 parts of a root extract of Sophora Root, 1 to 4 parts of a fruit extract of Tribulus Terrestris, and 0.5 to 2 parts of a fruit extract of Cornus Cherry.

2. A plant extract having a redness-removing effect according to claim 1, characterized in that it contains, by mass, 3 parts of Sophora Root Extract, 2 parts of Tribulus Terrestris Fruit Extract, and 1 part of Cornus Cherry Fruit Extract, and / or 85 to 95 parts of butanediol and 2 to 6 parts of water.

3. 3. The plant extract having redness-reducing activity according to claim 2, characterized in that the plant extract contains, by mass, 90 parts of butanediol and 4 parts of water.

4. The Sophora Root Extract is an aqueous ethanol extract of Sophora Root, The Tribulus terrestris fruit extract is an aqueous ethanol extract of Tribulus terrestris fruit, 2. The plant extract having a redness-reducing effect according to claim 1, wherein the extract of Cornus japonica fruit is an aqueous ethanol extract of Cornus japonica fruit.

5. The plant extract having a redness-reducing effect according to claim 4, characterized in that the volume fraction of ethanol in the aqueous ethanol solution is 90% to 95%.

6. A method for producing a plant extract having a redness-removing effect according to any one of claims 1 to 5, characterized in that it comprises a step of extracting the root of Sophora Root, the fruit of Tribulus Terrestris and the fruit of Cornus Cherry at 65 to 85°C for 1 to 3 hours, using an aqueous ethanol solution as an extraction solvent and a ratio of raw material to solvent of 1:10 to 1:

30.

7. The method for producing a plant extract having a redness-reducing effect according to claim 6, characterized in that it comprises a step of concentrating the extract obtained by the extraction and redissolving it in 1,3-butanediol.

8. A method for producing a plant extract having a redness-removing effect as described in claim 6, characterized in that it includes a step of fumigating the root of Sophora Root, fruit of Tribulus Terrestris, and fruit of Cornus Cherry prior to the extraction.

9. 9. The method for producing a plant extract having a redness-reducing effect according to claim 8, wherein the fumigation treatment time is 20 to 40 minutes.

10. The ratio of the raw material to the solvent is 1:15 to 1:20; and / or the temperature of the extraction is 75 to 85°C; And / or, the method for producing a plant extract having redness-reducing effect described in claim 6, characterized in that the extraction time is 2 to 3 hours.

11. The method for producing a plant extract having a redness-removing effect according to claim 6, characterized in that the mass ratio of the root of Sophora Root, the fruit of Tribulus Terrestris, and the fruit of Cornus Cherry is (15-60):(10-40):(5-20).

12. A method for producing a plant extract having redness-removing effect as described in claim 11, characterized in that the mass ratio of the Sophora Root, the Tribulus Terrestris fruit and the Cornus Fruit is 3:2:

1.

13. 8. The method for producing a plant extract having a redness-reducing effect according to claim 7, wherein the amount of 1,3-butanediol used in the reconstitution is 10 to 25 times the weight of the herb based on the amount of the herb.

14. The method for producing a plant extract having a redness-reducing effect according to claim 13, characterized in that the amount of 1,3-butanediol used is 10 to 16 times the weight of the herb.

15. The method for producing a plant extract having a redness-reducing effect according to claim 7, characterized in that the redissolution comprises adding 1,3-butanediol in multiple batches and performing a concentration treatment after each addition.

16. The method for producing a plant extract having a redness-reducing effect according to claim 15, characterized in that the 1,3-butanediol is added in 3 to 5 portions.

17. 17. The use of a plant extract having a redness-reducing effect according to any one of claims 1 to 5 or a plant extract produced by the method for producing a plant extract having a redness-reducing effect according to any one of claims 6 to 16 in the production of skin care products.

18. 18. The application of claim 17, wherein the skin care product comprises a skin care product for sensitive skin.

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