Plant nutrient composition and organic fertilizer fabricated by fertilizing the same

Abstract

A plant nutrient composition according to one embodiment of the present invention comprises a first composition comprising an animal amino acid preparation and a second composition that enhances antioxidant efficacy, wherein the two compositions are fermented. Here, the second composition includes two or more by-products among soybean by-products, almond by-products, spinach by-products, napa cabbage by-products, or cabbage by-products.

Description

Technology Field

[0001] A plant nutrient composition and a mixed organic fertilizer produced by fermenting the same are disclosed. Background Technology

[0003] Research on the resource recovery of organic waste or by-products is attracting significant interest both domestically and internationally, as it can simultaneously prevent environmental pollution and produce energy.

[0004] Among organic waste or by-products, those generated during food manufacturing or processing remain difficult to dispose of continuously. As food production is conducted on a factory scale, waste and by-products are often discarded rather than utilized due to factors such as transportation costs, labor expenses, and spoilage during transport and storage. In particular, food by-products containing high levels of organic matter decompose easily during the summer, causing severe pollution such as foul odors. Consequently, food manufacturers and processors face significant difficulties in disposing of these by-products and generally rely on waste disposal contractors, paying a fixed collection fee for their disposal.

[0005] For example, in the manufacturing process of soybean processed foods, about 13 kg of soybean pulp with a moisture content of 80% or more can be produced per 10 kg of soybeans. Although these by-products are rich in nutrients such as protein, fat, and carbohydrates, they have the problem of spoiling very easily due to the proliferation of bacteria caused by the high moisture content.

[0006] Furthermore, for example, in the case of spinach, napa cabbage, and Chinese cabbage, a large amount of by-products or waste can be generated during the processing. Additionally, in the case of almonds, a large amount of by-products or waste, such as almond shells or outer skins, can be generated during the manufacturing of processed foods.

[0007] Therefore, it is necessary to identify and effectively utilize the nutritional components, active ingredients, etc. contained in the by-products or waste generated from the aforementioned food manufacturing or processing processes.

[0008] Meanwhile, demand for anti-aging, skin improvement, and maintaining skin elasticity has recently increased significantly, and antioxidant efficacy is receiving attention to realize these functionalities. Therefore, there is a need to enhance the antioxidant efficacy of crops such as fruits and vegetables consumed by people.

[0009] A related prior art document is Korean Registered Patent No. 10-1187327. The problem to be solved

[0011] One embodiment of the present invention is to provide a plant nutrient composition and a mixed organic fertilizer that significantly enhance the antioxidant effect of the applied crop.

[0012] One embodiment of the present invention is to provide a plant nutrient composition and a mixed organic fertilizer that promotes the growth and rooting of crops.

[0013] One embodiment of the present invention is to provide a plant nutrient composition and a mixed organic fertilizer that minimizes damage from pests and diseases to crops.

[0014] One embodiment of the present invention is to provide a plant nutrient composition and a mixed organic fertilizer that minimize cold damage to crops.

[0015] One embodiment of the present invention is to provide a plant nutrient composition and a mixed organic fertilizer that improve the sugar content of crops.

[0016] In addition to the above-mentioned tasks, embodiments according to the present invention may be used to achieve other tasks not specifically mentioned. means of solving the problem

[0018] A plant nutrient composition according to one embodiment of the present invention comprises a first composition comprising an animal amino acid preparation and a second composition that enhances antioxidant efficacy, wherein the two compositions are fermented.

[0019] Here, the second composition includes two or more by-products among soybean by-products, almond by-products, spinach by-products, napa cabbage by-products, or cabbage by-products.

[0020] Based on the total weight of the plant nutrient composition, the second composition may be 10 to 30 weight percent.

[0021] Animal amino acid preparations may contain one or more of tuna processing by-products or jellyfish waste.

[0022] Animal amino acid preparations may further contain one or more of livestock blood, blood meal, and bone meal.

[0023] The fermentation composition may be fermented by one or more microorganisms among microorganisms of the genus Bacillus, microorganisms of the genus Rhodobacter, or microorganisms of the genus Rhizopus.

[0024] The second composition includes soybean by-products, almond by-products, spinach by-products, napa cabbage by-products, and cabbage by-products, and the content ratio of soybean by-products, almond by-products, spinach by-products, napa cabbage by-products, and cabbage by-products may be 1:1:1:1:1.

[0025] It may further include one or more of trehalose, betaine, or seaweed extract.

[0026] It may contain more bamboo vinegar.

[0027] A mixed organic fertilizer according to one embodiment of the present invention can be produced by fermenting the plant nutrient composition. Effects of the invention

[0029] A plant nutrient composition and mixed organic fertilizer according to one embodiment of the present invention can significantly enhance the antioxidant effect of the applied crop, promote crop growth and rooting, minimize crop damage from pests and diseases and cold damage, and improve crop sugar content. Brief explanation of the drawing

[0031] Figure 1a is a graph showing the DPPH radical scavenging activity (%) of plant nutrient composition samples according to Examples 1 to 4. FIG. 1b is a graph showing μM TE / 100 mL samples of plant nutrient composition samples according to Examples 1 to 4. FIG. 2a is a graph showing the DPPH radical scavenging activity (%) of plant nutrient composition samples according to Comparative Examples 1 to 4. FIG. 2b is a graph showing the μM TE / 100 mL sample of the plant nutrient composition samples according to Comparative Examples 1 to 4. Specific details for implementing the invention

[0032] Embodiments of the present invention are described in detail with reference to the attached drawings so that those skilled in the art can easily implement the invention. The present invention may be embodied in various different forms and is not limited to the embodiments described herein. In the drawings, parts unrelated to the explanation have been omitted to clearly explain the invention, and the same reference numerals are used for identical or similar components throughout the specification. Furthermore, specific descriptions of widely known prior art are omitted.

[0033] In the drawing, the thickness is enlarged to clearly represent various layers and regions. When a part such as a layer, film, region, or plate is described as being "above" another part, this includes not only the case where it is "immediately above" another part, but also the case where there is another part in between. On the other hand, when a part is described as being "immediately above" another part, it means that there is no other part in between. Conversely, when a part such as a layer, film, region, or plate is described as being "below" another part, this includes not only the case where it is "immediately below" another part, but also the case where there is another part in between. On the other hand, when a part is described as being "immediately below" another part, it means that there is no other part in between.

[0034] Throughout the specification, when a part is described as "including" a certain component, this means that, unless specifically stated otherwise, it does not exclude other components but may include additional components.

[0035] Throughout the specification, "composition" refers to a substance in which two or more components are uniformly mixed, and is a concept that includes not only finished products but also intermediate materials for manufacturing finished products.

[0037] The plant nutrient composition according to the embodiments can significantly enhance the antioxidant effect of the applied crop. In addition, the plant nutrient composition can promote the growth and rooting of the applied crop, minimize damage from pests and diseases, minimize cold damage, and improve the sugar content of the applied crop.

[0038] In addition, the plant nutrient composition according to the embodiments can reduce environmental pollution by utilizing discarded food processing by-products or waste.

[0039] The plant nutrient composition according to the example comprises a fermented composition in which a first composition containing an animal amino acid preparation and a second composition enhancing antioxidant efficacy are fermented.

[0040] The animal amino acid preparation included in the first composition may include, for example, tuna by-products, jellyfish waste, etc.

[0041] Tuna by-products can be, for example, tuna processing by-products.

[0042] Tuna processing by-products may be by-products of the processing (steaming) process during the canning process of tuna. The processing process may be, for example, a process in which frozen tuna is thawed, the head, internal organs, etc. are removed, and then steaming (steaming) and cleaning operations are performed.

[0043] Tuna processing by-products include most parts of the tuna, such as the head, skin, tail, bones, blood meat, and belly meat.

[0044] Tuna processing by-products contain not only diverse and abundant amino acids but also various nutrients such as Na, P, K, Fe, Mg, Al, Cu, and Ca, and because tuna bones are processed together, the by-products can contain very high amounts of calcium (Ca) and phosphorus (P).

[0045] The belly meat contains a large amount of vitamin C, the blood meat contains a large amount of vitamin B1 and vitamin B2, and the skin meat and tail contain a large amount of unsaturated fatty acids such as DHA and EPA.

[0046] In addition, unlike farmed fish, tuna is safe from the harmful effects of antibiotics and chemicals, and the above-mentioned boiling process can be hygienic as it is performed at high temperatures.

[0047] Therefore, a plant nutrient composition containing tuna processing by-products can supply a large amount of various nutrients to the applied crop.

[0048] A plant nutrient composition containing tuna processing by-products provides significant assistance to crop growth and the development of roots, stems, and fruit set. Due to the amino acids contained in the tuna by-products, it can improve the taste and gloss of crops, increase resistance to cold damage, and suppress the occurrence, survival, and hatching of pests, thereby preventing secondary contamination, promoting plant growth, and strengthening disease resistance. Furthermore, due to the large amount of trace elements, it can strengthen crop roots and stems, thicken leaves, reinforce the function of fruit peels, and improve the vibrancy and color of flower petals, thereby enhancing marketability, shelf life, and storage life.

[0049] The adult body of a jellyfish is 94–98% water, and the main component of the jellyfish is gelatin, a protein. The mucin component that gives the jellyfish its slipperiness is mainly composed of chondroitin, which is a combination of protein and carbohydrate, and chondroitin can function to retain moisture in plants.

[0050] Since jellyfish cause damage to fishing activities by getting caught in fixed nets, trawls, and gill nets, and deplete fish resources, and also cause industrial damage such as clogging cooling water intakes in nuclear and thermal power plants and coastal industrial areas, utilizing such jellyfish waste can minimize environmental pollution and industrial damage and improve economic efficiency.

[0051] Animal amino acid preparations may further include one or more of livestock blood, blood meal, and bone meal.

[0052] Livestock blood is very rich in protein and can be formulated into blood meal or liquid form through a fermentation process for convenient use. This livestock blood can be formulated into a nano-sized colloidal form to increase the free amino acid content.

[0053] In the case of blood meal and bone meal, the protein content is very rich, and the trace element content can also be high.

[0054] The second composition can significantly enhance the antioxidant effect of the crop to which it is applied. The second composition includes two or more by-products selected from soybean by-products, almond by-products, spinach by-products, Chinese cabbage by-products, or cabbage by-products.

[0055] Soybean, almond, spinach, Napa cabbage, or cabbage byproducts are generally discarded; however, upcycling them into ingredients for plant nutrients can reduce environmental pollution. Furthermore, since these byproducts can be economically sourced and processed into raw materials, excellent economic feasibility can be achieved.

[0056] Soybean by-products, almond by-products, spinach by-products, napa cabbage by-products, and kale by-products can significantly enhance the antioxidant efficacy of crops when a plant nutrient composition is applied to crops.

[0057] When such an antioxidant efficacy-enhancing composition is fermented together with the first composition, the antioxidant efficacy can be further enhanced by a synergistic effect.

[0058] Based on the total weight of the plant nutrient composition, the second composition may be about 10 to 30 weight percent, and within this range, the antioxidant efficacy of the applied crop may be further enhanced.

[0059] When the second composition includes all of the soybean by-product, almond by-product, spinach by-product, napa cabbage by-product, and cabbage by-product, the content ratio of the soybean by-product, almond by-product, spinach by-product, napa cabbage by-product, and cabbage by-product may be, for example, 1:1:1:1:1.

[0060] Soybean byproducts are rich in various nutrients and bioactive substances, including free amino acids, gamma-aminobutyric acid (GABA), isoflavones, and soyasaponins, which can promote the growth of applicable crops and enhance antioxidant effects.

[0061] Almond by-products also contain large amounts of active ingredients, including free amino acids, vitamin E, and calcium, and can enhance the antioxidant efficacy of the crops to which they are applied.

[0062] Spinach by-products contain various vitamins and minerals as active ingredients, and contain a large amount of active ingredients that produce antioxidant effects.

[0063] Napa cabbage byproducts and Chinese cabbage byproducts contain large amounts of polyphenols and flavonoids, which are antioxidant substances, and can significantly enhance the antioxidant performance of the crops used.

[0064] A plant nutrient composition is prepared by fermenting a mixture of the first composition and the second composition.

[0065] Here, the microorganisms used for fermentation may be, for example, one or more microorganisms among microorganisms of the genus Bacillus, microorganisms of the genus Rhodobacter, or microorganisms of the genus Rhizopus.

[0066] When the microorganism used for fermentation is a Bacillus genus microorganism, it may be Bacillus sp. CMB26, but is not limited thereto. CMB26 is an antagonistic microorganism that produces liopeptides capable of destroying fungal spores and mycelia.

[0067] The plant nutrient composition may further include bamboo vinegar.

[0068] Bamboo vinegar contains growth-promoting hormones such as natural auxin, gibberellin, cytokinin, and abscisic acid as physiologically active components, which can promote cell growth and root development, and exhibit sterilization performance.

[0069] The plant nutrient composition may further include one or more of trehalose, betaine, or seaweed extract. Such trehalose, betaine, or seaweed extract may further enhance the cold damage prevention effect.

[0070] In addition, the plant nutrient composition may include various known compositions to realize specific functionality. For example, it may include mineral solutions for promoting the growth of the applied crop.

[0071] A plant nutrient composition can be manufactured by the following processes.

[0072] First, a step is performed in which a first composition containing an animal amino acid preparation and a second composition are mixed and crushed using a conventional wet crusher, etc.

[0073] Next, the crushed mixed composition and molasses are introduced into a stirring tank equipped with a boiler device, stirred for about 1 to 3 hours at a temperature of about 60 to 80°C, then transferred to a cooling tank, stirred again for about 20 to 30 hours at a temperature of about 30 to 50°C, and then transferred to a fermentation tank.

[0074] Next, Bacillus sp. CMB26 strain is inoculated into the composition transferred to a fermentation tank, and after introducing oxygen, the mixture is fermented and aged for about one year. Then, it is left to stand for about 45 to 55 hours to precipitate the solids and collect the supernatant. Bamboo vinegar is added to the collected supernatant, and the mixture is stirred at a temperature of about 20 to 40°C for about 20 to 30 hours to prepare a plant nutrient composition.

[0075] The mixed organic fertilizer according to the example can be produced by fermenting the aforementioned plant nutrient composition.

[0076] Mixed organic fertilizers can significantly enhance the antioxidant effects of applied crops. In addition, mixed organic fertilizers can promote crop growth and root development, minimize damage from pests and diseases, reduce cold damage, and improve the sugar content of the crops.

[0077] These plant nutrient compositions and mixed organic fertilizers can be applied to fruits such as apples, pears, tomatoes, cherry tomatoes, melons, mangoes, grapes, tangerines, persimmons, strawberries, peaches, bananas, pineapples, watermelons, and plums; and vegetables such as spinach, napa cabbage, cucumbers, eggplants, chili peppers, perilla leaves, lettuce, cabbage, iceberg lettuce, chives, onions, radishes, sweet potatoes, sugar beets, potatoes, broccoli, green mustard greens, Dolsan mustard greens, carrots, garlic, chives, radish sprouts, and colorful vegetables.

[0079] The present invention will be described below through examples and experimental examples. However, the present invention is not limited by the following examples and experimental examples.

[0081] Example 1

[0082] 300 kg of tuna processing by-products (supplied from a tuna canning factory in Mokpo), 500 kg of seawater, 40 kg of soybean by-products (supplied from a tofu manufacturing factory in Mokpo), 40 kg of almond by-products (purchased through the Korea Agro-Fisheries Food Trade Corporation), 40 kg of spinach by-products (supplied from a sorting facility in Sinan), 40 kg of napa cabbage by-products (supplied from a kimchi processing factory in Yeongam), and 40 kg of cabbage by-products (supplied from a kimchi processing factory in Yeongam) are mixed to produce 1,000 kg of a mixed composition. The mixture is placed into a wet crusher (crushing nozzle 8 mm) and crushed. Subsequently, 1,000 kg of the crushed mixed composition and 80 kg of molasses are introduced into a stirring tank equipped with a boiler device, stirred at a temperature of 70 ℃ for 2 hours, transferred to a cooling tank, stirred again at a temperature of 40 ℃ for 24 hours, and then transferred to a fermentation tank. Next, Bacillus sp. CMB26 strain is inoculated, oxygen is introduced, and the mixture is fermented and aged for about one year. Afterward, it is left for 48 hours to allow the solids to settle and the supernatant is collected. 40 kg of bamboo vinegar is added to the collected supernatant, and the mixture is stirred at a temperature of 30 ℃ for 24 hours to prepare a plant nutrient composition.

[0083] After centrifuging the plant nutrient composition (4,000 rpm, 10 min), the supernatant is filtered through a Whatman No. 2 filter and diluted with deionized water to prepare a sample with a concentration of 5%.

[0085] Example 2

[0086] The prepared plant nutrient composition is prepared in the same manner as in Example 1, except that after centrifuging (4,000 rpm, 10 min) the prepared plant nutrient composition, the supernatant is filtered through a Whatman No. 2 filter and then diluted with deionized water to prepare a sample with a concentration of 10%.

[0088] Example 3

[0089] The prepared plant nutrient composition is prepared in the same manner as in Example 1, except that after centrifuging (4,000 rpm, 10 min) the prepared plant nutrient composition, the supernatant is filtered through a Whatman No. 2 filter and then diluted with deionized water to prepare a sample with a concentration of 15%.

[0091] Example 4

[0092] The prepared plant nutrient composition is prepared in the same manner as in Example 1, except that after centrifuging (4,000 rpm, 10 min) the prepared plant nutrient composition, the supernatant is filtered through a Whatman No. 2 filter and then diluted with deionized water to prepare a sample with a concentration of 20%.

[0094] Comparative Example 1

[0095] A composition of 1,000 kg is prepared by including 300 kg of tuna processing by-products (supplied from a tuna canning factory in Mokpo) and 700 kg of seawater. The mixture is placed into a wet crusher (crushing nozzle 8 mm) and crushed. Subsequently, 1,000 kg of the crushed mixed composition and 80 kg of molasses are introduced into a stirring tank equipped with a boiler device. After stirring for 2 hours at a temperature of 70°C, the mixture is transferred to a cooling tank, stirred again for 24 hours at a temperature of 40°C, and then transferred to a fermentation tank. Bacillus sp. CMB26 is inoculated into the fermentation composition, oxygen is introduced, and the mixture is fermented and aged for approximately one year. It is then left to stand for 48 hours to allow the solids to settle, and the supernatant is collected. 40 kg of bamboo vinegar is added to the collected supernatant, and the mixture is stirred for 24 hours at a temperature of 30°C to produce a plant nutrient composition.

[0096] After centrifuging the plant nutrient composition (4,000 rpm, 10 min), the supernatant is filtered through a Whatman No. 2 filter and diluted with deionized water to prepare a sample with a concentration of 5%.

[0098] Comparative Example 2

[0099] The prepared plant nutrient composition is prepared in the same manner as Comparative Example 1, except that the prepared composition is centrifuged (4,000 rpm, 10 min), the supernatant is filtered through a Whatman No. 2 filter, and then diluted with deionized water to prepare a sample with a concentration of 10%.

[0101] Comparative Example 3

[0102] The prepared plant nutrient composition is prepared in the same manner as Comparative Example 1, except that after centrifuging (4,000 rpm, 10 min) the prepared plant nutrient composition, the supernatant is filtered through a Whatman No. 2 filter and then diluted with deionized water to prepare a sample with a concentration of 15%.

[0104] Comparative Example 4

[0105] The prepared plant nutrient composition is prepared in the same manner as Comparative Example 1, except that the prepared composition is centrifuged (4,000 rpm, 10 min), the supernatant is filtered through a Whatman No. 2 filter, and then diluted with deionized water to prepare a sample with a concentration of 20%.

[0107] Experimental Example 1 - Antioxidant Effect

[0108] An experiment was performed to compare the antioxidant effects of plant nutrient composition samples according to Examples 1 to 4 and Comparative Examples 1 to 4. 200 g was taken from each plant nutrient composition prepared according to Examples 1 to 4 and Comparative Examples 1 to 4 and used as each sample.

[0109] The DPPH Assay method was used.

[0110] Specifically, 24 mg of DPPH reagent was dissolved in 100 mL of methanol and stored at -20 °C, and the DPPH solution was adjusted to an absorbance of 0.98 ± 0.02 at 517 nm using methanol before the test.

[0111] 1.5 mL of DPPH solution was added to 50 μL of each sample and reacted for 30 minutes in the dark at room temperature, and the absorbance was measured at 517 nm using a UV-Vis spectrophotometer (Libra S70, Biochrom, UK).

[0112] The measurement results are shown in Table 1 and Figures 1a to 2b below.

[0113] In Table 1 and Figures 1a to 2b, the DPPH radical scavenging activity (%) of the samples was calculated using the following formula.

[0114] Scavenging activity (%) = [(ABS Control - ABS Sample) / ABS Control ] x 100

[0116] Under the same conditions, a standard curve was constructed using Trolox, and the concentration of Trolox exhibiting the same antioxidant activity as the sample was calculated and expressed as μM TE (Trolox equivalent) / 100 mL sample.

[0118] Sample Test Results DPPH radical scavenging activity (%) μM TE / 100 mL sample Example 1 22.71 ± 0.46 33.85 ± 0.67 Example 2 40.78 ± 0.42 59.44 ± 0.58 Example 3 56.30 ± 0.39 81.04 ± 0.55 Example 4 71.19 ± 1.47 101.55 ± 2.02 Comparative Example 1 36.79 ± 0.23 0.82 ± 0.16 Comparative Example 2 64.98 ± 0.51 4.23 ± 0.39 Comparative Example 3 83.52 ± 0.49 11.00 ± 0.55 Comparative Example 4 91.59 ± 0.48 13.55 ± 0.41

[0120] Referring to Table 1 and Figures 1a to 2b, when comparing the μM TE (Trolox equivalent) / 100 mL sample values ​​representing antioxidant activity at the same concentration, it can be confirmed that the sample according to Example 1 has significantly superior antioxidant activity compared to the sample according to Comparative Example 1, the sample according to Example 2 has significantly superior antioxidant activity compared to the sample according to Comparative Example 2, the sample according to Example 3 has significantly superior antioxidant activity compared to the sample according to Comparative Example 3, and the sample according to Example 4 has significantly superior antioxidant activity compared to the sample according to Comparative Example 4.

[0122] Although preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention as defined in the following claims also fall within the scope of the present invention.

Claims

Claim 1 A plant nutrient composition comprising a first composition containing an animal amino acid preparation and a second composition that enhances antioxidant efficacy, wherein the second composition comprises a soybean byproduct, an almond byproduct, a spinach byproduct, a Chinese cabbage byproduct, and a cabbage byproduct, wherein the content ratio of the soybean byproduct, the almond byproduct, the spinach byproduct, the Chinese cabbage byproduct, and the cabbage byproduct is 1:1:1:1:1, and the antioxidant activity value compared to the standard substance Trolox exceeds 33 μM TE / 100 mL. Claim 2 A plant nutrient composition according to claim 1, wherein the second composition is 10 to 30 weight% based on the total weight of the plant nutrient composition. Claim 3 In claim 1, the animal amino acid preparation is a plant nutrient composition comprising one or more of tuna processing by-products or jellyfish waste. Claim 4 In paragraph 3, the animal amino acid preparation is a plant nutrient composition further comprising one or more of livestock blood, blood meal, and bone meal. Claim 5 In claim 1, the fermented composition is a plant nutrient composition fermented by one or more microorganisms selected from Bacillus, Rhodobacter, or Rhizopus. Claim 6 delete Claim 7 A plant nutrient composition according to claim 1, further comprising one or more of trehalose, betaine, or seaweed extract. Claim 8 A plant nutrient composition comprising bamboo vinegar in addition to the above in claim 1. Claim 9 A mixed organic fertilizer produced by fermenting a plant nutrient composition according to any one of paragraphs 1 to 5, 7, or 8.

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