Selective antimicrobial agent and / or selective microbicidal agent, selective antimicrobial composition and / or selective microbicidal composition, and use thereof

Abstract

Provided are: a selective antimicrobial agent and / or a selective microbicidal agent containing ozone-treated glycerin, having antibacterial and / or bactericidal actions on harmful microorganisms, and not having antimicrobial and / or microbicidal actions on non-harmful microorganisms; and a selective antimicrobial composition and / or a selective microbicidal composition.

Description

Selective antimicrobial agents and / or selective microbiotacitants, selective antimicrobial compositions and / or selective microbiotacitants, and their uses Related applications

[0001] This application claims priority to Japanese Patent Application No. 2024-204694, filed on 25 November 2024, and incorporates all the provisions contained herein.

[0002] The present invention relates to selective antimicrobial agents and / or selective microbiotacitants, selective antimicrobial compositions and / or selective microbiotacitants, and uses thereof.

[0003] It is known that approximately 400 to 700 types of bacteria inhabit the human oral cavity. These bacteria form specific bacterial communities in various locations, such as the tooth surface, the tongue surface, and periodontal pockets. Among these bacteria, those known as periodontal disease bacteria form a biofilm called dental plaque on the tooth surface and in periodontal pockets. When these periodontal disease bacteria further proliferate within this biofilm, periodontitis, gingivitis, and periodontitis develop. Inhibiting biofilm formation through drug treatment or regularly removing dental plaque through physical cleaning such as brushing is important for reducing the proportion of periodontal disease bacteria in the oral cavity, and is crucial for preventing and improving the symptoms of periodontal disease.

[0004] As a method for preventing and improving the symptoms of periodontal disease, disinfectants, bactericides, or antibiotics are sometimes injected into periodontal pockets using, for example, an oral syringe or an ultrasonic scaler. Examples of disinfectants and bactericides used include chlorhexidine gluconate (CHG), cetylpyridinium chloride (CPC), and benzethonium chloride (BTC). CHG has been reported to cause discoloration, taste disturbances, and tartar formation, and other drugs have also been reported to cause hypersensitivity. Furthermore, the use of antibiotics raises concerns about AMR (antibiotic-resistant bacteria), as they may be ineffective against resistant bacteria, or long-term use may lead to the development of resistant bacteria. Disinfectants also raise concerns about toxicity and side effects.

[0005] Streptococcus mitis and Streptococcus oralis are known as beneficial bacteria in the oral cavity. Among them, Streptococcus mitis is sometimes called the Bifidobacterium of the large intestine, and it accounts for about 90% of the commensal bacteria in the oral cavity, making its presence important for a healthy mouth. However, there is a problem in that beneficial bacteria are the first to be killed when antibiotics or disinfectants are used. Therefore, there is a need for ingredients that do not kill beneficial bacteria, but only act on harmful bacteria such as periodontal disease bacteria.

[0006] It is becoming clear that the relative proportions of bacterial species that make up the gut microbiota (also known as the "gut flora") play a crucial role in maintaining health. Although the gut microbiota is a relatively stable ecosystem, it is known that its composition and activity can change under the influence of various factors. For example, if the balance of the gut microbiota is disrupted due to stress, aging, etc., it may affect one's health.

[0007] Vaginal and vulvar candidiasis is a fungal infection of the vaginal and vulvar mucosa, primarily caused by Candida albicans. Symptoms include vaginitis and vulvitis, characterized by severe itching and a characteristic increase in vaginal discharge. It is crucial that medications used to treat vaginal and vulvar candidiasis possess not only excellent antibacterial activity against Candida but also low irritation to the mucous membranes, given their direct application. Traditionally, antibiotic use in treating vaginal and vulvar candidiasis reduces the normal flora of the vagina and vulva, including Lactobacillus casei. Furthermore, steroid use in treating Candida inflammation increases reinfection, leading to a resurgence of Candida even after the inflammation subsides. Additionally, the vagina has a 40-50 times higher absorption rate than the skin, limiting the number of suitable medications available.

[0008] The present invention has been made in view of the prior art described above, and aims to provide selective antimicrobial agents and / or selective microbiotacitants, selective antimicrobial compositions and / or selective microbiotacitants, and uses thereof, which have excellent antimicrobial and / or microbiotacitating effects against harmful microorganisms but do not have antimicrobial and / or microbiotacitating effects against non-harmful microorganisms.

[0009] The means for solving the aforementioned problems are as follows: The selective antimicrobial agent and / or selective microbiotaicide of the present invention contains ozone-treated glycerin and has antimicrobial and / or microbiotacitating activity against harmful microorganisms, but does not have antimicrobial and / or microbiotacitating activity against non-harmful microorganisms. Preferably, the ozone-treated glycerin in the selective antimicrobial agent and / or selective microbiotaicide has an oxidizing power of 1 ppm to 2,000 ppm in terms of ozone concentration.

[0010] The selective antimicrobial composition and / or selective microbiotacitative composition of the present invention contains ozone-treated glycerin and has antimicrobial and / or microbiotacitative activity against harmful microorganisms, but does not have antimicrobial and / or microbiotacitative activity against non-harmful microorganisms. Preferably, the ozone-treated glycerin in the selective antimicrobial composition and / or selective microbiotacitative composition has an oxidizing power of 1 ppm to 2,000 ppm based on ozone concentration. Preferably, the selective antimicrobial composition and / or selective microbiotacitative composition contains polyethylene glycol (PEG) with an average molecular weight of 400 or more.

[0011] The selective antimicrobial composition and / or selective microbial agent for injection into periodontal pockets of the present invention is used by injection into the periodontal pocket. Preferably, the harmful microorganisms in the selective antimicrobial composition and / or selective microbial agent for injection into periodontal pockets are periodontal disease bacteria, and the non-harmful microorganisms are oral commensal bacteria other than periodontal disease bacteria.

[0012] The selective antimicrobial composition and / or selective microbial killer composition for oral application of the present invention is used by applying it to the oral cavity. Preferably, the harmful microorganisms in the selective antimicrobial composition and / or selective microbial killer composition for oral application are periodontal disease bacteria, caries-causing bacteria, or bad breath-causing bacteria, and the non-harmful microorganisms are non-pathogenic commensal bacteria in the oral cavity.

[0013] The selective antimicrobial composition and / or selective microbial killer composition for vaginal and vulvar application of the present invention is used by applying it to the vagina and vulva. Preferably, the harmful microorganism in the selective antimicrobial composition and / or selective microbial killer composition for vaginal and vulvar application is a Candida fungus, and the non-harmful microorganism is a non-pathogenic commensal bacterium of the vagina and vulva.

[0014] The selective antimicrobial composition and / or selective microbial composition for improving the intestinal flora of the present invention is used by oral administration or ingestion.

[0015] The cosmetic composition of the present invention contains the composition of the present invention.

[0016] The food and beverage products of the present invention contain the composition of the present invention.

[0017] The oral composition of the present invention contains the composition of the present invention.

[0018] The animal cleaning composition of the present invention contains the composition of the present invention.

[0019] The present invention provides selective antimicrobial agents and / or selective microbiotacitants, selective antimicrobial compositions and / or selective microbiotacitants, and uses thereof, which have excellent antimicrobial and / or microbiotacitative activity against harmful microorganisms but do not have antimicrobial and / or microbiotacitative activity against non-harmful microorganisms.

[0020] Figure 1 shows the results of antimicrobial and / or bactericidal tests using OG200 on Enterococcus faecalis. Figure 2 shows the results of antimicrobial and / or bactericidal tests using OG200 on Lactobacillus casei. Figure 3 shows the antimicrobial and / or bactericidal activity against Candida albicans.

[0021] (Selective antimicrobial agents and / or selective microbactericidal agents, selective antimicrobial compositions and / or selective microbactericidal compositions) The selective antimicrobial agents and / or selective microbactericidal agents of the present invention contain ozone-treated glycerin and, if necessary, other components. Depending on the type of microorganism targeted, the selective antimicrobial agents and / or selective microbactericidal agents of the present invention may be referred to as selective antimicrobial agents and / or selective bactericidal agents.

[0022] The selective antimicrobial composition and / or selective microbiotacitating composition of the present invention preferably contains ozone-treated glycerin and polyethylene glycol (PEG) with an average molecular weight of 400 or more, and further contains other components as needed. Depending on the type of microorganism targeted, the selective antimicrobial composition and / or selective microbiotacitating composition of the present invention may be referred to as a selective antimicrobial composition and / or selective bactericidal composition.

[0023] "Antimicrobial and / or microbiota" is synonymous with "antibacterial and / or bactericidal," and means antimicrobial, microbiota, or both.

[0024] The selective antimicrobial agent and / or selective microbiotacitating agent of the present invention, and the selective antimicrobial composition and / or selective microbiotacitating composition of the present invention, have excellent antimicrobial and / or microbiotacitating activity against harmful microorganisms, but do not have antimicrobial and / or microbiotacitating activity against non-harmful microorganisms. Harmful means pathogenic to humans, and also includes animals other than humans. Examples of animals include pets such as cats, dogs, and rabbits; livestock such as cattle, horses, pigs, sheep, and goats; and poultry such as ducks, chickens, and geese. Examples of microorganisms include bacteria, fungi, molds, yeasts, viruses, and protozoa. Among these, bacteria and fungi are particularly targeted.

[0025] <Ozone-treated glycerin> Ozone-treated glycerin preferably has an oxidizing power of 1 ppm to 2,000 ppm in terms of ozone concentration, more preferably 10 ppm to 1,500 ppm, even more preferably 10 ppm to 1,000 ppm, even more preferably 10 ppm to 500 ppm, particularly preferably 10 ppm to 200 ppm, even more particularly preferably 50 ppm to 200 ppm, and even more particularly preferably 50 ppm to 100 ppm. The oxidizing power of ozone-treated glycerin in terms of ozone concentration can be measured, for example, by potassium iodide titration.

[0026] Ozone-treated glycerin is produced by gas-liquid contact between a glycerin solution and a gas containing ozone. The glycerin concentration in the glycerin solution is not particularly limited and can be appropriately selected depending on the purpose. For example, a glycerin concentration of 50% by mass or more is preferred, 80% by mass or more is more preferred, 90% by mass or more is even more preferred, 95% by mass or more is particularly preferred, and 98% by mass or more is most preferred. Specifically, a glycerin solution of 84% by mass or more of the Japanese Pharmacopoeia product is preferred, a concentrated glycerin solution of 98% by mass or more of the Japanese Pharmacopoeia product is more preferred, and a purified glycerin solution of 98.5% by mass or more of the Japanese Pharmacopoeia product is even more preferred. In the glycerin solution, the solvent for glycerin is not particularly limited and can be an aqueous solvent such as water.

[0027] The gas containing ozone preferably has a high concentration of ozone. The method for producing a gas with a high ozone concentration is not particularly limited; for example, a silent discharge type ozone generator that produces ozone by silently discharging oxygen gas can be used. When using oxygen gas, for example, a medical oxygen cylinder may be used, or oxygen produced by an oxygen generator may be used.

[0028] The method for bringing a glycerin solution into gas-liquid contact with an ozone-containing gas is not particularly limited. For example, one method involves placing the glycerin solution in a tank and releasing the gas with a high ozone concentration into the tank as fine bubbles using a diffuser. Specifically, by aerating the glycerin solution with a gas containing approximately 37,000 ppm of ozone for a predetermined time, ozone-treated glycerin with an oxidizing power of 1 ppm to 2,000 ppm (based on ozone concentration) can be obtained.

[0029] The aeration time (gas-liquid contact time) is not particularly limited and may be, for example, 1 hour or more, 5 hours or more, 10 hours or more, 15 hours or more, 24 hours or more or 1 day or more, 48 hours or more or 2 days or more, 72 hours or more or 3 days or more, 120 hours or more or 5 days or more, or 168 hours or more or 7 days or more. The upper limit of the predetermined aeration time is not particularly limited but may be, for example, 720 hours or less or 30 days or less, or 360 hours or less or 15 days or less.

[0030] As the ozone-treated glycerin, it is also possible to use ozone-treated glycerin with an oxidizing power of 1 ppm to 1,000 ppm obtained by diluting high-oxidizing-power ozone-treated glycerin with an oxidizing power of 2,000 ppm or more with a polyol or water. The oxidizing power of high-oxidizing-power ozone-treated glycerin, in terms of ozone concentration, is preferably 2,000 ppm or more, more preferably 2,500 ppm or more, and even more preferably 3,000 ppm or more. As the polyol, at least one selected from glycerin, butylene glycol, propylene glycol, pentylene glycol, and polyethylene glycol is preferred, glycerin, butylene glycol, propylene glycol, or pentylene glycol is more preferred, and glycerin is particularly preferred. There are no particular restrictions on the dilution method; a polyol dilution can be obtained by mixing high-oxidizing-power ozone-treated glycerin and polyol in a predetermined ratio and stirring.

[0031] Ozone-treated glycerin preferably contains a cyclic peroxide represented by the following chemical formula (1) or a salt thereof.

[0032]

[0033] The cyclic peroxide represented by the above chemical formula (1) is the case where, in the following chemical formula (I), R １１ are all hydrogen atoms.

[0034] In the above chemical formula (I), each R １１ may be the same as or different from each other, and each is a hydrogen atom, a hydroxyl group, a hydroxyalkyl group, or an alkyl group. In the above hydroxyalkyl group or alkyl group, the number of carbon atoms is preferably 1 to 10, more preferably 1 to 5, and still more preferably 1 to 3. Examples of the above alkyl group include a methyl group, an ethyl group, and a propyl group.

[0035] When the cyclic peroxide represented by the above chemical formula (1) can form a salt, the salt may be an acid addition salt or a base addition salt. Further, the acid forming the acid addition salt may be an inorganic acid or an organic acid, and the base forming the base addition salt may be an inorganic base or an organic base.

[0036] The above inorganic acid is not particularly limited, and examples thereof include sulfuric acid, phosphoric acid, hydrofluoric acid, hydrochloric acid, hydrobromic acid, hydroiodic acid, hypofluorous acid, hypochlorous acid, hypobromous acid, hypoiodous acid, fluorous acid, chlorous acid, bromous acid, iodous acid, fluoric acid, chloric acid, bromic acid, iodic acid, perfluoric acid, perchloric acid, perbromic acid, and periodic acid. The above organic acid is not particularly limited, and examples thereof include p-toluenesulfonic acid, methanesulfonic acid, oxalic acid, p-bromobenzenesulfonic acid, carbonic acid, succinic acid, citric acid, benzoic acid, and acetic acid.

[0037] The inorganic base is not particularly limited, and examples thereof include ammonium hydroxide, alkali metal hydroxides, alkaline earth metal hydroxides, carbonates, hydrogen carbonates, etc. More specifically, examples include sodium hydroxide, potassium hydroxide, potassium carbonate, sodium carbonate, sodium hydrogen carbonate, potassium hydrogen carbonate, calcium hydroxide, calcium carbonate, etc. The organic base is not particularly limited, and examples thereof include ethanolamine, triethylamine, tris(hydroxymethyl)aminomethane, etc. The production method of these salts is also not particularly limited, and for example, they can be produced by appropriately adding the above-mentioned acids or bases to the compound by a known method.

[0038] The cyclic peroxide represented by the above chemical formula (1) is not particularly limited, but can be produced by subjecting glycerin to ozone oxidation. The cyclic peroxide represented by the above chemical formula (1) may be used as it is without being separated from the reaction mixture (for example, the ozone-treated glycerin solution), or may be used after separating the cyclic peroxide represented by the above chemical formula (1) from the reaction mixture. The separation method is not particularly limited, and for example, the cyclic peroxide represented by the above chemical formula (1) can be separated from the reaction mixture by chromatography such as preparative thin layer chromatography.

[0039] There are no particular restrictions on other components in the selective antimicrobial agent and / or selective microbicide of the present invention, and they can be appropriately selected according to the purpose. Examples include thickeners, emulsifiers, preservatives, antioxidants, colorants, powder components, surfactants, polyols, water, etc.

[0040] The content of ozone-treated glycerin in the selective antimicrobial agent and / or selective microbiotaspinal agent of the present invention is preferably 0.1% to 100% by mass, more preferably 0.5% to 100% by mass, even more preferably 1% to 100% by mass, even more preferably 10% to 100% by mass, particularly preferably 30% to 100% by mass, even more preferably 50% to 100% by mass, even more preferably 80% to 100% by mass, and most preferably 90% to 100% by mass, based on the total amount of the selective antimicrobial agent and / or selective microbiotaspinal agent.

[0041] The content of ozone-treated glycerin in the selective antimicrobial composition and / or selective microbial composition of the present invention is preferably 0.1% to 99% by mass, more preferably 0.5% to 95% by mass, even more preferably 0.5% to 90% by mass, even more preferably 1% to 90% by mass, particularly preferably 10% to 90% by mass, even more preferably 30% to 90% by mass, even more preferably 50% to 90% by mass, and most preferably 70% to 90% by mass, based on the total amount of the selective antimicrobial composition and / or selective microbial composition.

[0042] The selective antimicrobial composition and / or selective microbiotacitative composition of the present invention preferably contains polyethylene glycol.

[0043] <Polyethylene Glycol> Polyethylene glycol (PEG) preferably has an average molecular weight of 400 or more, more preferably 1,000 or more, even more preferably 1,000 to 20,000, even more preferably 1,000 to 10,000, and particularly preferably 3,000 to 8,000. The "average molecular weight" of polyethylene glycol refers to the molecular weight calculated from the terminal hydroxyl value, and is the average molecular weight described in the Quasi-Drug Raw Materials Standards 2021 (Value measured by the measurement method described in the Quasi-Drug Raw Materials Standards 2021). The polyethylene glycol content is preferably 0.1% to 20% by mass, more preferably 0.5% to 15% by mass, and even more preferably 1% to 10% by mass, based on the total amount of the selective antimicrobial composition and / or selective microbiotacitating composition.

[0044] <Other Components> There are no particular limitations on the other components in the selective antimicrobial composition and / or selective microbiotaicidal composition of the present invention, and they can be appropriately selected depending on the purpose. Examples include thickeners, emulsifiers, preservatives, antioxidants, colorants, powder components, surfactants, polyols other than polyethylene glycol, and water.

[0045] Examples of thickening agents include gum arabic, carrageenan, karaya gum, tragacanth gum, carob gum, quince seed (marmelo), casein, dextrin, gelatin, sodium pectinate, sodium araginate, methylcellulose, ethylcellulose, CMC, hydroxyethylcellulose, hydroxypropylcellulose, polyvinyl alcohol (PVA), polyvinylpyrrolidone (PVP), sodium polyacrylate, carboxyvinyl polymer, locust bean gum, guar gum, tamarind gum, dialkyldimethylammonium sulfate cellulose, xanthan gum, aluminum magnesium silicate, bentonite, hectorite, aluminum magnesium silicate (bee gum), laponite, and anhydrous silicic acid. These may be used individually or in combination of two or more.

[0046] As for the water used, water used in cosmetics, quasi-drugs, etc., can be used, such as purified water, deionized water, or tap water.

[0047] Examples of polyols include butylene glycol, propylene glycol, pentylene glycol, ethylene glycol, propylene glycol, trimethylene glycol, 1,2-butylene glycol, 1,3-butylene glycol, tetramethylene glycol, 2,3-butylene glycol, pentamethylene glycol, 2-butene-1,4-diol, hexylene glycol, and octylene glycol. These may be used individually or in combination of two or more.

[0048] (Selective antimicrobial composition and / or selective microbiotacitative composition for periodontal pocket injection) The selective antimicrobial composition and / or selective microbiotacitative composition for periodontal pocket injection of the present invention contains the selective antimicrobial agent and / or selective microbiotacitative agent, or selective antimicrobial composition and / or selective microbiotacitative composition of the present invention, and further contains other components as necessary. The content of the selective antimicrobial agent and / or selective microbiotacitative agent, or selective antimicrobial composition and / or selective microbiotacitative composition of the present invention in the selective antimicrobial composition and / or selective microbiotacitative composition for periodontal pocket injection is not particularly limited and can be adjusted as appropriate.

[0049] The selective antimicrobial composition and / or selective microbial agent composition for periodontal pocket injection of the present invention is used by injection into the periodontal pocket. The selective antimicrobial composition and / or selective microbial agent composition for periodontal pocket injection can be filled into an injection syringe and directly injected into the periodontal pocket using the injection syringe.

[0050] The selective antimicrobial composition and / or selective microbial composition for injection into periodontal pockets possesses a selective antimicrobial and / or bactericidal effect, having antibacterial and / or bactericidal activity against harmful microorganisms while not having antibacterial and / or bactericidal activity against non-harmful microorganisms. Specifically, the harmful microorganisms are periodontal disease bacteria (bad bacteria), and the non-harmful microorganisms are non-pathogenic oral commensal bacteria (good bacteria).

[0051] Examples of periodontal disease bacteria include Porphyromonas gingivalis, Tannerella forsythia (formerly known as Tannerella forsythia), Treponema denticola, Campylobacter gracilis, Campylobacter rectus, Campylobacter showe, Eubacterium nodatum, and Fusobacterium nucleatum. Examples include *Cylindrium nucleatum*, *Fusobacterium polymorphum*, *Prevotella intermedia*, *Prevotella nigressens*, *Peptostreptococcus micros*, and *Streptococcus constellatus*.

[0052] Examples of non-pathogenic commensal bacteria in the oral cavity include Streptococcus oralis, Streptococcus mitis, Streptococcus gordoniai, and Neisseria subflaba.

[0053] (Selective antimicrobial composition and / or selective microbiotacitative composition for intraoral application) The selective antimicrobial composition and / or selective microbiotacitative composition for intraoral application of the present invention contains the selective antimicrobial agent and / or selective microbiotacitative agent, or selective antimicrobial composition and / or selective microbiotacitative composition of the present invention, and further contains other components as necessary. The content of the selective antimicrobial agent and / or selective microbiotacitative agent, or selective antimicrobial composition and / or selective microbiotacitative composition of the present invention in the selective antimicrobial composition and / or selective microbiotacitative composition for intraoral application is not particularly limited and can be adjusted as appropriate.

[0054] The selective antimicrobial composition and / or selective microbial agent for oral application of the present invention is used by applying it to the oral cavity. The selective antimicrobial composition and / or selective microbial agent for oral application is used, for example, for stomatitis, gingivitis, periodontitis, and suture sites in implant surgery.

[0055] The selective antimicrobial composition and / or selective microbial killer composition for intraoral application has antibacterial and / or bactericidal activity against harmful microorganisms, while possessing a selective antibacterial and / or bactericidal effect that does not have antibacterial and / or bactericidal activity against non-harmful microorganisms. Specifically, the harmful microorganisms are periodontal disease bacteria, caries-causing bacteria, or bad breath-causing bacteria, and the non-harmful microorganisms are non-pathogenic oral commensal bacteria (beneficial bacteria). The periodontal disease bacteria and non-pathogenic oral commensal bacteria are the same as those in the selective antimicrobial composition and / or selective microbial killer composition for injection into periodontal pockets described above.

[0056] Examples of bacteria that cause dental caries include Streptococcus mutans, Streptococcus sobrinus, and Actinomyces naeslundii. Examples of bacteria that cause bad breath include Fusobacterium nucleatum and Veillonella dispar.

[0057] (Selective antimicrobial composition and / or selective microbial killer composition for vaginal and vulvar application) The selective antimicrobial composition and / or selective microbial killer composition of the present invention contains the selective antimicrobial agent and / or selective microbial killer, or selective antimicrobial composition and / or selective microbial killer composition of the present invention, and further contains other components as necessary. The content of the selective antimicrobial agent and / or selective microbial killer, or selective antimicrobial composition and / or selective microbial killer composition of the present invention in the selective antimicrobial composition and / or selective microbial killer composition for vaginal and vulvar application is not particularly limited and can be adjusted as appropriate.

[0058] The selective antimicrobial composition and / or selective microbial agent for vaginal and vulvar application of the present invention is used by applying it into the vagina. The selective antimicrobial composition and / or selective microbial agent for vaginal and vulvar application has antibacterial and / or bactericidal activity against harmful microorganisms, but does not have antibacterial and / or bactericidal activity against non-harmful microorganisms, thus possessing a selective antibacterial and / or bactericidal effect. Specifically, it is preferable that the harmful microorganisms are Candida fungi (bad bacteria), and the non-harmful microorganisms are non-pathogenic commensal bacteria (good bacteria) of the vagina and vulva.

[0059] Examples of Candida fungi include Candida albicans, Candida glabrata, Candida tropicalis, Candida parapsilosis, and Candida krusei.

[0060] Examples of non-pathogenic commensal bacteria in the vagina and vulva include Lactobacillus casei, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus plantarum, and Lactobacillus crispatus.

[0061] Vaginal mucosa is frequently infected with Candida albicans, but antibiotic use reduces Lactobacillus casei. Furthermore, using steroids for Candida albicans inflammation makes the body more susceptible to infection, and even if the inflammation subsides, Candida albicans will increase again. Additionally, the vagina has a 40-50 times higher absorption rate than the skin, limiting the number of usable medications. The selective antimicrobial and / or selective microbial composition for vaginal and vulvar application, with an oxidizing power of 10 ppm (based on ozone concentration) of ozone-treated glycerin, exhibits antibacterial and / or bactericidal effects against Candida albicans. This 10 ppm oxidizing power is expected to provide an ideal selective antibacterial and / or bactericidal effect without killing the normal flora of the vagina and vulva.

[0062] (Selective antimicrobial composition and / or selective microbiotacitative composition for improving intestinal flora) The selective antimicrobial composition and / or selective microbiotacitative composition for improving intestinal flora of the present invention contains the selective antimicrobial agent and / or selective microbiotacitative agent, or selective antimicrobial composition and / or selective microbiotacitative composition of the present invention, and further contains other components as necessary. The content of the selective antimicrobial agent and / or selective microbiotacitative agent, or selective antimicrobial composition and / or selective microbiotacitative composition of the present invention in the selective antimicrobial composition and / or selective microbiotacitative composition for improving intestinal flora is not particularly limited and can be adjusted as appropriate.

[0063] The selective antimicrobial composition and / or selective microbial composition for improving the intestinal flora of the present invention is used by oral administration or ingestion. The selective antimicrobial composition and / or selective microbial composition for improving the intestinal flora has an antibacterial and / or bactericidal effect against harmful microorganisms, but does not have an antibacterial and / or bactericidal effect against non-harmful microorganisms, thus possessing a selective antibacterial and / or bactericidal effect. Specifically, it is preferable that the harmful microorganisms are Clostridium perfringens, Escherichia coli (toxic strain), Staphylococcus aureus, or Bacteroides, and the non-harmful microorganisms are non-pathogenic intestinal commensal bacteria (beneficial bacteria). Examples of non-pathogenic intestinal commensal bacteria include Bifidobacterium and Lactobacillus (lactic acid bacilli).

[0064] (Cosmetics) The cosmetics of the present invention contain the selective antimicrobial agent and / or selective microbiotaspinal agent, or selective antimicrobial composition and / or selective microbiotaspinal composition of the present invention, and further contain other components as necessary.

[0065] The content of the selective antimicrobial agent and / or selective microbiotaspinal agent, or selective antimicrobial composition and / or selective microbiotaspinal composition of the present invention in a cosmetic composition is not particularly limited and can be adjusted as appropriate.

[0066] <Other Ingredients> The cosmetic composition of the present invention may be manufactured by appropriately blending other ingredients, such as anionic surfactants, cationic surfactants, amphoteric surfactants, nonionic surfactants, humectants, water-soluble polymers, thickeners, film-forming agents, UV absorbers, metal ion chelating agents, lower alcohols, polyhydric alcohols, oils, sugars, amino acids, organic amines, polymer emulsions, pH adjusters, skin nutrients, vitamins, plant extracts, antioxidants, antioxidant aids, fragrances, colorants, preservatives, disinfectants, water, etc., as needed, within the limits that do not impair the effects of the present invention.

[0067] Examples of cationic surfactants include alkyltrimethylammonium salts (e.g., stearyltrimethylammonium chloride, behenyltrimethylammonium chloride, etc.); alkylpyridinium salts (e.g., cetylpyridinium chloride, etc.); distearyldimethylammonium chloride, dialkyldimethylammonium salts; poly(N,N'-dimethyl-3,5-methylenepiperidinium) chloride; alkylquaternary ammonium salts; alkyldimethylbenzylammonium salts; alkylisoquinolinium salts; dialkylmolyphonium salts; POE-alkylamines; alkylamine salts; polyamine fatty acid derivatives; amyl alcohol fatty acid derivatives; benzalkonium chloride; and benzethonium chloride. These may be used individually or in combination of two or more.

[0068] Examples of amphoteric surfactants include imidazoline-based amphoteric surfactants (e.g., 2-undecyl-N,N,N-(hydroxyethylcarboxymethyl)-2-imidazoline sodium, 2-cocoyl-2-imidazolinium hydroxide-1-carboxyethyloxy disodium salt, 2-heptadecyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, etc.); and betaine-based surfactants (e.g., lauryldimethylaminoacetic acid betaine, alkyl betaine, alkylamide betaine, alkyl sulfobetaine, etc.). These may be used individually or in combination of two or more.

[0069] Examples of lipophilic nonionic surfactants include sorbitan fatty acid esters (e.g., sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan sesquioleate, sorbitan trioleate, sorbitan penta-2-ethylhexyl diglycerol sorbitan, sorbitan tetra-2-ethylhexyl diglycerol sorbitan, etc.); glycerin polyglycerin fatty acids (e.g., monocottonseed oil fatty acid glycerin, monoerucate glycerin, sesquioleate glycerin, monostearate glycerin, α,α'-oleate pyroglutamate glycerin, monostearate glycerin malic acid, etc.); propylene glycol fatty acid esters (e.g., propylene glycol monostearate, etc.); hydrogenated castor oil derivatives; and glycerin alkyl ethers. These may be used individually or in combination of two or more.

[0070] Examples of hydrophilic nonionic surfactants include POE-sorbitan fatty acid esters (e.g., POE-sorbitan monooleate, POE-sorbitan monostearate, POE-sorbitan monooleate, POE-sorbitan tetraoleate, etc.); POE-sorbitol fatty acid esters (e.g., POE-sorbitol monolaurate, POE-sorbitol monooleate, POE-sorbitol pentaoleate, POE-sorbitol monostearate, etc.); and POE-glycerol fatty acid esters (e.g., POE-glycerol monostearate). POE-monoleates such as POE-glycerin monoisostearate and POE-glycerin triisostearate; POE-fatty acid esters (e.g., POE-distearate, POE-monodiooleate, ethylene glycol distearate); POE-alkyl ethers (e.g., POE-lauryl ether, POE-oleyl ether, POE-stearyl ether, POE-behenyl ether, POE-2-octyldodecyl ether, POE-cholestanol ether); Pluronic® products; POE-POP- Alkyl ethers (e.g., POE / POP-cetyl ether, POE / POP-2-decyltetradecyl ether, POE / POP-monobutyl ether, POE / POP-hydrogenated lanolin, POE / POP-glycerin ether, etc.); tetraPOE / tetraPOP-ethylenediamine condensates (e.g., Tetronic, etc.); POE-castor oil hydrogenated castor oil derivatives (e.g., POE-castor oil, POE-hydrogenated castor oil, POE-hydrogenated castor oil monoisostearate, POE-hydrogenated castor oil triisostearate, POE-hydrogenated castor oil monopyroglyphate) Diesterine monoisostearate of tamic acid, POE-hydrogenated castor oil maleic acid, etc.; POE-beeswax / lanolin derivatives (e.g., POE-sorbitol beeswax, etc.); alkanolamides (e.g., coconut oil fatty acid diethanolamide, lauric acid monoethanolamide, fatty acid isopropanolamide, cocamide methyl monoethanolamide, etc.); POE-propylene glycol fatty acid esters; POE-alkylamines; POE-fatty acid amides; diethylene glycol laurate; sucrose fatty acid esters; alkylethoxydimethylamine oxide;Examples include trioleyl phosphate. These may be used individually or in combination of two or more.

[0071] Examples of humectants include glycerin, sugar alcohols, polyethylene glycol, propylene glycol, dipropylene glycol, 1,3-butylene glycol, chondroitin sulfate, hyaluronic acid, mucoitin sulfate, carotenoid acid, atelocollagen, cholesteryl-12-hydroxystearate, sodium lactate, bile salts, dl-pyrrolidone carboxylate, alkylene oxide derivatives, short-chain soluble collagen, diglycerin (EO)PO adduct, Rosa rugosa extract, Achillea millefolium extract, and Melilotus extract. These may be used individually or in combination of two or more.

[0072] Examples of natural water-soluble polymers include plant-derived polymers (e.g., gum arabic, tragacanth gum, galactan, guar gum, carob gum, karaya gum, carrageenan, pectin, agar, quince seed, algae colloid (cassia extract), starch (rice, corn, potato, wheat), glycyrrhizic acid); microbial polymers (e.g., xanthan gum, dextran, succinoglucan, pullulan, etc.); and animal-derived polymers (e.g., collagen, casein, albumin, gelatin, etc.). These may be used individually or in combination of two or more.

[0073] Examples of semi-synthetic water-soluble polymers include starch-based polymers (e.g., carboxymethyl starch, methylhydroxypropyl starch, etc.); cellulose-based polymers (methylcellulose, ethylcellulose, methylhydroxypropylcellulose, hydroxyethylcellulose, sodium cellulose sulfate, hydroxypropylcellulose, carboxymethylcellulose, sodium carboxymethylcellulose, crystalline cellulose, cellulose powder, etc.); and alginate-based polymers (e.g., sodium alginate, propylene glycol alginate, etc.). These may be used individually or in combination of two or more.

[0074] Examples of water-soluble polymers used in synthesis include vinyl polymers (e.g., polyvinyl alcohol, polyvinyl methyl ether, polyvinylpyrrolidone, carboxyvinyl polymer, etc.); polyoxyethylene polymers (e.g., polyethylene glycol 20,000, 40,000, 600,000 polyoxyethylene polyoxypropylene copolymers, high-polymer polyethylene glycol, etc.); acrylic polymers (e.g., sodium polyacrylate, polyethyl acrylate, polyacrylamide, etc.); polyethyleneimines; and cationic polymers. These may be used individually or in combination of two or more.

[0075] Examples of thickening agents include gum arabic, carrageenan, karaya gum, tragacanth gum, carob gum, quince seed (marmelo), casein, dextrin, gelatin, sodium pectinate, sodium araginate, methylcellulose, ethylcellulose, CMC, hydroxyethylcellulose, hydroxypropylcellulose, PVA, PVM, PVP, sodium polyacrylate, carboxyvinyl polymer, locust bean gum, guar gum, tamarind gum, dialkyldimethylammonium sulfate cellulose, xanthan gum, aluminum magnesium silicate, bentonite, hectorite, aluminum magnesium silicate (bee gum), laponite, and anhydrous silicic acid. These may be used individually or in combination of two or more.

[0076] Examples of UV absorbers include benzoic acid-based UV absorbers (e.g., para-aminobenzoic acid (hereinafter abbreviated as PABA), PABA monoglycerol ester, N,N-dipropoxy PABA ethyl ester, N,N-diethoxy PABA ethyl ester, N,N-dimethyl PABA ethyl ester, N,N-dimethyl PABA butyl ester, N,N-dimethyl PABA ethyl ester, etc.); anthranilic acid-based UV absorbers (e.g., homomenthyl-N-acetylanthranilate, etc.); and salicylic acid-based UV absorbers (e.g., amyl salicylate). , menthyl salicylate, homomenthyl salicylate, octyl salicylate, phenyl salicylate, benzyl salicylate, p-isopropanolphenyl salicylate, etc.); cinnamic acid-based UV absorbers (e.g., octyl methoxycinnamate, ethyl-4-isopropyl cinnamate, methyl-2,5-diisopropyl cinnamate, ethyl-2,4-diisopropyl cinnamate, methyl-2,4-diisopropyl cinnamate, propyl-p-methoxycinnamate, isopropyl-p-methoxycinnamate, isoamyl-p-methyl Toxicinnamete, octyl-p-methoxycinnamate (2-ethylhexyl-p-methoxycinnamate), 2-ethoxyethyl-p-methoxycinnamate, cyclohexyl-p-methoxycinnamate, ethyl-α-cyano-β-phenylcinnamate, 2-ethylhexyl-α-cyano-β-phenylcinnamate, glyceryl mono-2-ethylhexanoyl-diparamethoxycinnamate, etc.; benzophenone-based UV absorbers (e.g., 2,4-dihydroxybenzophenone, 2,2'-dihydroxy-4-methoxybenzophenone) (e.g., phenone, 2,2'-dihydroxy-4,4'-dimethoxybenzophenone, 2,2',4,4'-tetrahydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxy-4'-methylbenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-phenylbenzophenone, 2-ethylhexyl-4'-phenylbenzophenone-2-carboxylate, 2-hydroxy-4-n-octoxybenzophenone, 4-hydroxy-3-carboxybenzophenone);3-(4'-methylbenzylidene)-d,l-camphor, 3-benzylidene-d,l-camphor; 2-phenyl-5-methylbenzoxazole; 2,2'-hydroxy-5-methylphenylbenzotriazole; 2-(2'-hydroxy-5'-t-octylphenyl) Examples include benzotriazole; 2-(2'-hydroxy-5'-methylphenylbenzotriazole; dibenzarazine; dianisioylmethane; 4-methoxy-4'-t-butyldibenzoylmethane; 5-(3,3-dimethyl-2-norbornylidene)-3-pentan-2-one, dimorpholinopyridazino; 2-ethylhexyl-2-cyano-3,3-diphenyl acrylate; 2,4-bis-{[4-(2-ethylhexyloxy)-2-hydroxy]-phenyl}-6-(4-methoxyphenyl)-(1,3,5)-triazine, etc. These may be used individually or in combination of two or more.

[0077] Examples of metal ion chelating agents include 1-hydroxyethane-1,1-diphosphonic acid, tetrasodium 1-hydroxyethane-1,1-diphosphonic acid, disodium edetate, trisodium edetate, tetrasodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid, phosphoric acid, citric acid, ascorbic acid, succinic acid, edetate, and trisodium ethylenediaminehydroxyethyltriacetate. These may be used individually or in combination of two or more.

[0078] Examples of lower alcohols include ethanol, propanol, isopropanol, isobutyl alcohol, and t-butyl alcohol. These may be used individually or in combination of two or more.

[0079] Examples of polyhydric alcohols include dihydric alcohols (e.g., ethylene glycol, propylene glycol, trimethylene glycol, 1,2-butylene glycol, 1,3-butylene glycol, tetramethylene glycol, 2,3-butylene glycol, pentamethylene glycol, 2-butene-1,4-diol, hexylene glycol, octylene glycol, etc.); trihydric alcohols (e.g., glycerin, trimethylolpropane, etc.); tetrahydric alcohols (e.g., pentaerythritol such as 1,2,6-hexanetriol, etc.); pentahydric alcohols (e.g., xylitol, etc.); hexahydric alcohols (e.g., sorbitol, mannitol, etc.); and polyhydric alcohol polymers (e.g., diethylene glycol, dipropylene glycol, triethylene (e.g., ethylene glycol, polypropylene glycol, tetraethylene glycol, diglycerin, polyethylene glycol, triglycerin, tetraglycerin, polyglycerin, etc.); divalent alcohol alkyl ethers (e.g., ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, ethylene glycol monophenyl ether, ethylene glycol monohexyl ether, ethylene glycol mono-2-methylhexyl ether, ethylene glycol isoamyl ether, ethylene glycol benzyl ether, ethylene glycol isopropyl ether, ethylene glycol dimethyl ether, ethylene glycol diethyl ether, ethylene glycol dibutyl ether, etc.);Dihydric alcohol alkyl ethers (e.g., diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol dimethyl ether, diethylene glycol diethyl ether, diethylene glycol butyl ether, diethylene glycol methyl ethyl ether, triethylene glycol monomethyl ether, triethylene glycol monoethyl ether, propylene glycol monomethyl ether, propylene glycol monoethyl ether, propylene glycol monobutyl ether, propylene glycol isopropyl ether, dipropylene glycol methyl ether, dipropylene glycol ethyl ether, dipropylene glycol butyl ether, etc.); dihydric alcohol ether esters (e.g., ethylene glycol monomethyl ether acetate, ethylene glycol monoethyl ether acetate, ethylene glycol monobutyl ether acetate, ethylene glycol monophenyl ether acetate, ethylene glycol diadivate, ethylene glycol di Succinates (e.g., diethylene glycol monoethyl ether acetate, diethylene glycol monobutyl ether acetate, propylene glycol monomethyl ether acetate, propylene glycol monoethyl ether acetate, propylene glycol monopropyl ether acetate, propylene glycol monophenyl ether acetate, etc.); glycerin monoalkyl ethers (e.g., xyl alcohol, cerakyl alcohol, batyl alcohol, etc.); sugar alcohols (e.g., sorbitol, maltitol, maltotriol) Examples include ose, mannitol, sucrose, erythritol, glucose, fructose, starch-derived sugars, maltose, xylitol, starch-derived sugar-reduced alcohols, etc.; glycolid; tetrahydrofurfuryl alcohol; POE-tetrahydrofurfuryl alcohol; POP-butyl ether; POP / POE-butyl ether; tripolyoxypropylene glycerin ether; POP-glycerin ether; POP-glycerin ether phosphate; POP / POE-pentaneerythritol ether, polyglycerin, etc. These may be used individually or in combination of two or more.

[0080] Examples of monosaccharides include trioses (e.g., D-glyceryl aldehyde, dihydroxyacetone, etc.); tetraoses (e.g., D-erythritol, D-erythritol, D-threose, erythritol, etc.); pentoses (e.g., L-arabinose, D-xylose, L-lyxose, D-arabinose, D-ribose, D-ribulose, D-xylulose, L-xylulose, etc.); hexoses (e.g., D-glucose, D-talose, D-busicose, D-galactose, D-fructose, L-galactose, L- Examples include mannose, D-tagatose, etc.; heptasaccharides (e.g., aldoheptose, hepros, etc.); octasaccharides (e.g., octulose, etc.); deoxysaccharides (e.g., 2-deoxy-D-ribose, 6-deoxy-L-galactose, 6-deoxy-L-mannose, etc.); aminosaccharides (e.g., D-glucosamine, D-galactosamine, sialic acid, aminouronic acid, muramic acid, etc.); and uronic acids (e.g., D-glucuronic acid, D-mannuronic acid, L-glucuronic acid, D-galacturonic acid, L-iduronic acid, etc.). These may be used individually or in combination of two or more.

[0081] Examples of oligosaccharides include sucrose, gunthianose, umbelliferose, lactose, planteose, isolichnoses, α,α-trehalose, raffinose, licnoses, umbilicin, stachyose, and vervascoses. These may be used individually or in combination of two or more.

[0082] Examples of polysaccharides include cellulose, quince seed, chondroitin sulfate, starch, galactan, dermatan sulfate, glycogen, gum arabic, heparan sulfate, hyaluronic acid, tragacanth gum, keratan sulfate, chondroitin, mucoitin sulfate, guar gum, dextran, keratosulfate, locust bean gum, succinoglucan, and carotenoid acid. These may be used individually or in combination of two or more.

[0083] Examples of amino acids include neutral amino acids (e.g., threonine, cysteine, etc.) and basic amino acids (e.g., hydroxylysine, etc.). Examples of amino acid derivatives include sodium acyl sarcosinate (sodium lauroyl sarcosinate), acyl glutamate, sodium acyl β-alanine, glutathione, and pyrrolidone carboxylic acid. These may be used individually or in combination of two or more.

[0084] Examples of organic amines include monoethanolamine, diethanolamine, triethanolamine, morpholine, triisopropanolamine, 2-amino-2-methyl-1,3-propanediol, and 2-amino-2-methyl-1-propanol. These may be used individually or in combination of two or more. Examples of polymer emulsions include acrylic resin emulsion, ethyl polyacrylate emulsion, acrylic resin liquid, polyacrylic alkyl ester emulsion, polyvinyl acetate resin emulsion, and natural rubber latex. These may be used individually or in combination of two or more.

[0085] Examples of pH adjusting agents include buffers such as citric acid, lactate-sodium lactate, citrate-sodium citrate, and succinic acid-sodium succinate. These may be used individually or in combination of two or more.

[0086] Examples of vitamins include vitamins A and B. １ , B ２ , B ６ Examples include C, E or their derivatives, pantothenic acid or its derivatives, and biotin. These may be used individually or in combination of two or more.

[0087] Examples of antioxidants include tocopherols, dibutylhydroxytoluene, butylhydroxyanisole, and gallic acid esters. These may be used individually or in combination of two or more.

[0088] Examples of antioxidants include phosphoric acid, citric acid, ascorbic acid, maleic acid, malonic acid, succinic acid, fumaric acid, kephalin, hexametaphosphorate, phytic acid, and ethylenediaminetetraacetic acid. These may be used individually or in combination of two or more.

[0089] Other possible ingredients include, specifically, preservatives (e.g., ethylparaben, butylparaben, chlorphenesin, phenoxyethanol, etc.); disinfectants (e.g., isopropylmethylphenol, benzalkonium chloride, benzethonium chloride, chlorhexidine gluconate, etc.); anti-inflammatory agents (e.g., glycyrrhizic acid derivatives, glycyrrhetinic acid derivatives, salicylic acid derivatives, hinokitiol, zinc oxide, allantoin, etc.); whitening agents (e.g., placenta extract, saxifrage extract, arbutin, etc.); various extracts (e.g., Phellodendron amurense, Coptis japonica, Lithospermum erythrorhizon, Paeonia lactiflora, Swertia japonica, Birch, Sage, Loquat, Carrot, Aloe vera, Mallow, Iris, Grape, Coix lacryma-jobi, Luffa gourd, Lily, Saffron, Cnidium officinale, and Ginger). Examples of ingredients include: cucumber, St. John's wort, ononis, garlic, chili pepper, dried tangerine peel, angelica root, seaweed, etc.; activators (e.g., royal jelly, photosensitizer, cholesterol derivatives, etc.); blood circulation promoters (e.g., nonylic acid valenylamide, benzyl nicotinate, β-butoxyethyl nicotinate, capsaicin, gingerol, cantharis tincture, ichthamol, tannic acid, α-borneol, tocopherol nicotinate, inositol hexanicotinate, cyclandelate, cinnarizine, trazoline, acetylcholine, verapamil, cepharanthine, γ-oryzanol, etc.); anti-seborrheic agents (e.g., sulfur, thianthol, etc.); and anti-inflammatory agents (e.g., tranexamic acid, thiotaurine, hypotaurine, etc.). These may be used individually or in combination of two or more.

[0090] Furthermore, metal sequestering agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid, and malic acid; caffeine, tannins, verapamil, tranexamic acid or its derivatives, various herbal extracts such as licorice, quince, and wintergreen; drugs such as tocopherol acetate, glycyrrhetinic acid, glycyrrhizic acid or its derivatives or salts; whitening agents such as vitamin C, magnesium ascorbate phosphate, ascorbate glucoside, arbutin, and kojic acid; amino acids such as arginine and lysine or their derivatives; and sugars such as fructose, mannose, erythritol, trehalose, and xylitol may also be added as appropriate. These may be used individually or in combination of two or more.

[0091] The cosmetic composition of the present invention has excellent safety, stability, and usability, and is suitably used as a skin (skin care) cosmetic and / or hair (hair care) cosmetic. Examples of skin care cosmetics include skin cosmetics such as lotions, emulsions, creams, serums, and masks; base cosmetics such as cleansers, facial washes, UV protectants, makeup, base lotions, and makeup base creams; makeup cosmetics such as emulsions, foundations in various formulations such as oil-based and solid forms, eye colors, and cheek colors; and body cosmetics such as hand creams, leg creams, neck creams, and body lotions.

[0092] Examples of hair care cosmetics include shampoos such as oil shampoos, cream shampoos, conditioning shampoos, anti-dandruff shampoos, color-treated shampoos, and rinse-in shampoos; rinses, treatments, hair packs, hair foams, hair mousses, hair sprays, hair mists, hair waxes, hair gels, water greases, setting lotions, color lotions, hair tonics, hair liquids, pomades, hair sticks, hair creams, hair blow dryers, split end coats, hair oils, permanent wave solutions, straightening solutions, oxidative hair dyes, hair bleaches, hair color pre-treatments, hair color after-treatments, perm pre-treatments, perm after-treatments, hair manicures, and hair growth products.

[0093] The cosmetic composition of the present invention is suitably applied to humans, but can also be applied to animals other than humans, as long as the respective effects are achieved. Examples of animals include pets such as cats, dogs, and rabbits; livestock such as cattle, horses, pigs, sheep, and goats; and poultry such as ducks, chickens, and geese.

[0094] (Food and beverages) The food and beverages of the present invention contain the selective antimicrobial agent and / or selective microbiotaspinal agent, or selective antimicrobial composition and / or selective microbiotaspinal composition of the present invention, and further contain other components as necessary.

[0095] The content of the selective antimicrobial agent and / or selective microbiotaspinal agent, or selective antimicrobial composition and / or selective microbiotaspinal composition of the present invention in food and beverages is not particularly limited and can be adjusted as appropriate.

[0096] Here, "food and beverages" refers to substances that pose little risk to human health and are ingested orally or through the gastrointestinal tract in normal social life, and is not limited to administrative classifications such as food, pharmaceuticals, or quasi-drugs. Therefore, "food and beverages" in this embodiment broadly includes general foods, health foods (functional foods and beverages), health functional foods (foods for specified health uses, nutritional functional foods), quasi-drugs, pharmaceuticals, etc., that are ingested orally. There are no particular restrictions on the content of the ozone-treated glycerin in the food and beverages, and it can be adjusted as appropriate.

[0097] <Other Ingredients> There are no particular restrictions on the other ingredients mentioned above, and they can be appropriately selected from auxiliary raw materials, additives, or other ingredients commonly used in the manufacture of food and beverages, depending on the purpose. Examples include glucose, fructose, sucrose, maltose, sorbitol, stevioside, rubusoside, corn syrup, lactose, oligosaccharides, xylitol, trehalose, palatinose, aspartame, acesulfame potassium, sucralose, saccharin salts, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid esters, polyglycerin fatty acid esters, sucrose fatty acid esters, sorbitan fatty acid esters, gum arabic, carrageenan, casein, gelatin, pectin, agar, B vitamins, nicotinamide, calcium pantothenate, amino acids, calcium salts, colorants, flavorings, and preservatives. These may be used individually or in combination of two or more. There are no particular restrictions on the amount of the other ingredients, and they can be appropriately selected according to the purpose.

[0098] The aforementioned food and beverages are not particularly limited, but specific examples include: beverages such as soft drinks, carbonated drinks, nutritional drinks, fruit drinks, and lactic acid drinks (including concentrated concentrates and powders for adjusting these beverages); frozen desserts such as ice cream, ice sherbet, and shaved ice; noodles such as soba, udon, vermicelli, gyoza wrappers, shumai wrappers, Chinese noodles, and instant noodles; confectionery such as candy, chewing gum, chocolate, tablets, snacks, biscuits, jelly, jam, cream, and baked goods; processed seafood and livestock products such as kamaboko, ham, and sausage; dairy products such as processed milk and fermented milk; oils and fats and processed oils such as salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, and dressings; seasonings such as sauces and dips; soups, stews, salads, prepared foods, and pickles; and various other forms of health and nutritional supplements; as well as tablets, capsules, and drinks.

[0099] The embodiments of the food and beverage may take the form of well-known foods or pharmaceuticals. For example, as a pharmaceutical, it may take the form of a powder, capsule, granule, tablet, liquid, or other oral drug. Alternatively, it may take the form of a regular food, such as jelly, syrup, candy, gum, soft drink, supplement, or other well-known food, or it may be mixed in a predetermined amount with other well-known foods.

[0100] There are no particular restrictions on the method of consumption of the aforementioned food and beverages, and the method can be appropriately selected depending on the purpose. Examples include oral administration, parenteral administration, and gastrointestinal administration. Among these, oral administration is preferred.

[0101] The food and beverages of the present invention are suitably applied to humans, but can also be applied to animals other than humans, as long as their respective effects are achieved. Examples of animals include pets such as cats, dogs, and rabbits; livestock such as cattle, horses, pigs, sheep, and goats; and poultry such as ducks, chickens, and geese.

[0102] (Oral Composition) The oral composition of the present invention contains the selective antimicrobial agent and / or selective microbiotaspinal agent, or selective antimicrobial composition and / or selective microbiotaspinal composition of the present invention, and further contains other components as necessary.

[0103] The content of the selective antimicrobial agent and / or selective microbiotaspinal agent, or selective antimicrobial composition and / or selective microbiotaspinal composition of the present invention in the oral composition is not particularly limited and can be adjusted as appropriate.

[0104] The application forms of oral compositions are not particularly limited and can be used, for example, as pharmaceuticals, quasi-drugs, or cosmetics. Known uses of oral compositions can be appropriately adopted. For example, possible uses include chewing agents, oral dissolving agents, oral disintegrating agents, tongue care agents, mouth fresheners, toothpastes, mouthwashes, rinses, liquid toothpastes, biofilm dispersants, bad breath preventatives, gum massage agents, oral moisturizing agents, tongue coating removers, oral application agents, oral disinfectants, throat disinfectants, oral and throat agents, periodontal disease treatment agents, denture attachment agents, denture coating agents, denture stabilizers, denture preservatives, denture cleaners, and implant care agents.

[0105] The dosage form of the oral composition is not particularly limited, and can be applied to ointments, pastes, pasta preparations, sprays, gels, liquids, suspensions, gums, etc., by including a solvent such as water or alcohol.

[0106] Oral compositions may contain other components besides those mentioned above, depending on the intended use, form, and application. Examples of such other components include antibacterial agents, anti-inflammatory agents, fragrances, humectants, abrasives, alcohols, thickeners, sweeteners, medicinal components, colorants, stabilizers, and pH adjusters. Other components that are known to be incorporated into oral compositions may be used. Oral compositions may contain one of the above-mentioned other components alone, or two or more in combination.

[0107] The oral compositions of the present invention are suitably applied to humans, but can also be applied to animals other than humans, as long as their respective effects are achieved. Examples of animals include pets such as cats, dogs, and rabbits; livestock such as cattle, horses, pigs, sheep, and goats; and poultry such as ducks, chickens, and geese.

[0108] (Animal Cleaning Composition) The animal cleaning composition of the present invention contains the selective antimicrobial agent and / or selective microbiota agent, or selective antimicrobial composition and / or selective microbiota composition of the present invention, and further contains other components as necessary.

[0109] The content of the selective antimicrobial agent and / or selective microbiotaspinal agent, or selective antimicrobial composition and / or selective microbiotaspinal composition of the present invention in the animal washing composition is not particularly limited and can be adjusted as appropriate.

[0110] Other components in animal cleaning compositions include, for example, water, anionic surfactants, cationic surfactants, amphoteric surfactants, nonionic surfactants, humectants, water-soluble polymers, thickeners, film-forming agents, UV absorbers, metal ion chelating agents, lower alcohols, polyhydric alcohols, oils, sugars, amino acids, organic amines, polymer emulsions, pH adjusters, skin nutrients, vitamins, plant extracts, antioxidants, antioxidant aids, fragrances, colorants, preservatives, disinfectants, insecticidal components, and insect repellent components.

[0111] Animals to which the animal cleaning composition can be applied include non-human animals such as pets like cats, dogs, and rabbits; livestock such as cattle, horses, pigs, sheep, and goats; and poultry such as ducks, chickens, and geese.

[0112] Examples of animal cleansing compositions include animal soap, animal shampoo, animal rinse-in shampoo, animal conditioner, animal lotion, animal medicated shampoo, animal hair tonic, and animal hair growth tonic.

[0113] Hereinafter, embodiments of the present invention will be described, but the present invention is not limited to these embodiments in any way. The content of each component is in mass % unless otherwise specified. In the following embodiments, the oxidizing power of ozone-treated glycerin in terms of ozone concentration was measured as follows.

[0114] <Measurement of Oxidizing Power> The oxidizing power of the ozone-treated glycerin solution was measured by the potassium iodide titration method. Specifically, 17.90 g of potassium dihydrogen phosphate (KH ２ PO ４ ), and 17.14 g of disodium hydrogen phosphate (Na ２ HPO ４ ·12H ２ O) were added to 400 g of purified water, dissolved, and then 25.00 g of potassium iodide (KI) was added and dissolved. The resulting solution was made up to 500 g in total with purified water to prepare a potassium iodide absorption solution. Next, 0.32 g of sodium thiosulfate (Na ２ S ２ O ３ ) was made up to 400 g in final weight with purified water and then dissolved in purified water to prepare sodium thiosulfate. Further, 0.22 g of starch was added to 20 g of purified water, boiled and dissolved to prepare a starch aqueous solution. Next, 50 g of the potassium iodide absorption solution was added to about 1 g (1.00 to 1.20 g) of the ozone-treated glycerin solution, and the mixture was stirred for 10 minutes on a stirrer set at a rotation speed of 300 rpm and a temperature of 0°C. After the stirring, 0.2 ml of the starch aqueous solution was added. Then, using a burette, the sodium thiosulfate solution was slowly dropped into the mixture after the addition of the starch aqueous solution until the color of the mixture became colorless and transparent. Then, based on the following formula (A), the oxidizing power of the sample was calculated. O = (St × 24 × T) / S... (A) O: Oxidizing power of the sample (ppm) St: Concentration of sodium thiosulfate in the sodium thiosulfate solution (mmol / L) S: Amount of the sample (g) T: Titration amount of the sodium thiosulfate solution (mL)

[0115] (Example 1 of Ozone-Treated Glycerin Production) High-concentration glycerin and ozone were brought into gas-liquid contact to produce high-oxidizing-power ozone-treated glycerin. A 50L Teflon® tank was used as the contact tank. A diffuser was installed on the bottom of the tank to allow ozone to be supplied into the tank as fine bubbles. As the ozone generator, a silent discharge type ozone generator (manufactured by Mediplus Pharmaceutical Co., Ltd.) with an ozone generation capacity of 100g per hour using oxygen with a concentration of 90% by volume or more as the raw material was used. 22 kg of high-concentration glycerin (glycerin with a concentration of 98% by mass or more according to the Japanese Pharmacopoeia) was placed in the tank, 20 L of oxygen per minute was supplied to the silent discharge type ozone generator, and the generated ozone-containing gas was released into the tank through the diffuser for 5 days to obtain ozone-treated glycerin (hereinafter sometimes referred to as "OG2000") with an oxidizing power of 2000 ppm based on ozone concentration. The obtained "OG2000" contained a cyclic peroxide represented by the above general formula (1).

[0116] (Production Example 2 of Ozone-Treated Glycerin) In the same manner as in Production Example 1 of Ozone-Treated Glycerin, except that the aeration conditions were changed to a one-day release period, ozone-treated glycerin (hereinafter sometimes referred to as "OG500") with an oxidizing power of 500 ppm based on ozone concentration was obtained.

[0117] (Production Example 3 of Ozone-Treated Glycerin) In the same manner as in Production Example 1 of Ozone-Treated Glycerin, except that the aeration conditions were changed to a 12-hour release period, ozone-treated glycerin (hereinafter sometimes referred to as "OG200") with an oxidizing power of 200 ppm based on ozone concentration was obtained.

[0118] (Production Example 4 of Ozone-Treated Glycerin) In the same manner as in Production Example 1 of Ozone-Treated Glycerin, except that the aeration conditions were changed to a 6-hour release period, ozone-treated glycerin (hereinafter sometimes referred to as "OG100") with an oxidizing power of 100 ppm based on ozone concentration was obtained.

[0119] (Production Example 5 of Ozone-Treated Glycerin) In the same manner as in Production Example 1 of Ozone-Treated Glycerin, except that the aeration conditions were changed to a 3-hour release period, ozone-treated glycerin (hereinafter sometimes referred to as "OG50") with an oxidizing power of 50 ppm based on ozone concentration was obtained.

[0120] (Production Example 6 of Ozone-Treated Glycerin) In the same manner as in Production Example 1 of Ozone-Treated Glycerin, except that the aeration conditions were changed to a 1-hour release, ozone-treated glycerin (hereinafter sometimes referred to as "OG10") with an oxidizing power of 10 ppm based on ozone concentration was obtained.

[0121] <Selective antimicrobial and / or bactericidal test 1> (1) Test substance: Ozone-treated glycerin (OG200) - Target substance: Concentrated glycerin solution (glycerin with a concentration of 98% by mass or higher of the Japanese Pharmacopoeia product)

[0122] (2) Test strain ・Staphylococcus oralis (NBRC113011) ・Staphylococcus mitis (NBRC106071) ・Porphyromonas gingivalis (NBRC115147)・Staphylococcus gordonii ・Enterococcus faecalis (ATCC29212) ・Lactobacillus casei (ATCC393)

[0123] (3) Test materials 1) Dilution solution for the stock sample: 50% glycerin aqueous solution, sterile water, sterile PBS (phosphate-buffered saline), reaction stop solution (0.02 mol / L sodium thiosulfate)

[0124] 2) Culture media (i) Culture medium for bacterial suspension preparation: Brainheart Infusion (BHI) agar medium or 5% sheep blood agar medium (for Porphyromonas) 52 g of Brainheart Infusion Agar (BD) was dissolved in 1 L of distilled water and autoclaved at 121°C for 15 minutes. The mixture was dispensed into 20 mL portions into petri dishes, solidified, and stored at 4°C until use. A commercially available culture medium (Nissui Plate Sheep Blood Agar Medium, manufactured by Nissui Pharmaceutical Co., Ltd.) was used for the blood agar medium. (ii) Liquid culture medium for post-sensitization: Brainheart Infusion (BHI) liquid medium 37 g of Brainheart Infusion Broth (BD) was dissolved in 1 L of distilled water and autoclaved at 121°C for 15 minutes. (iii) Confirmation agar medium: Brainheart Infusion (BHI) agar medium or 5% sheep blood agar medium

[0125] 3) Sample preparation For Staphylococcus oralis, Staphylococcus mitis, Porphyromonas gingivaris, and Staphylococcus gordonii, ozone-treated glycerin (OG200; oxidizing power 200 ppm) was used, and OG200 was diluted with sterile water to an oxidizing power of 100 ppm based on ozone concentration, and then diluted with 50% glycerin aqueous solution to achieve oxidizing powers of 50 ppm, 10 ppm, 1 ppm, and 0.1 ppm based on ozone concentration. For Enterococcus faecalis and Lactobacillus casei, OG200 was diluted with a 50% glycerin aqueous solution so that the oxidizing power, converted to ozone concentration, was 100 ppm, 50 ppm, 10 ppm, 1 ppm, and 0.1 ppm.

[0126] 4) Preparation of bacterial suspension: The bacteria were incubated at 37°C for 24 hours in a culture medium for bacterial suspension preparation. Using the cultured bacteria, the solution was prepared by adjusting the concentration in sterile PBS to a McFarland ratio of 0.5, and this was used as the inoculum.

[0127] 5) Antibacterial and / or bactericidal effect test (i) Dispense 0.9 mL each of the ozone-treated glycerin of each oxidizing power prepared in 3.3) above into sterile small test tubes. (ii) Add 0.1 mL each of the bacterial suspension prepared in 3.4) above to the small test tubes containing ozone-treated glycerin. (iii) Sensitize the bacterial suspension for (1 minute, 5 minutes, 10 minutes, 60 minutes, or 120 minutes). (iv) After sensitization, transfer the entire sensitized solution from 5) (iii) above into a small test tube containing 20 μL of 0.02 mol / L sodium thiosulfate, and then transfer 0.1 mL of this solution to a small test tube containing 0.9 mL of liquid medium for post-sensitization culture or liquid medium for pre-culture, according to the bacterial species. The pre-culture conditions for each bacterial species were as follows. (v) After pre-culture, the viability of each bacterial species was confirmed using a confirmation agar medium appropriate for each bacterial species.

[0128] (4) Test Results The antibacterial and / or bactericidal effects of ozone-treated glycerin on each bacterium are shown in Tables 1 to 6. The results for Enterococcus faecalis and Lactobacillus casei are shown in Figures 1 and 2. No inhibition of bacterial growth was observed in S. mitis, S. oralis, and S. gordonii (S. gordonii showed growth inhibition with an oxidizing power of 200 ppm at 120 minutes). For P. gingivaris, the oxidizing power was 200 ppm at sensitization times of 1 minute and 5 minutes, 50 ppm at 10 minutes, 10 ppm at 60 minutes, and 1 ppm at 120 minutes. Furthermore, no growth inhibition was observed in Enterococcus faecalis and Lactobacillus casei (growth inhibition concentration: ≥100 ppm).

[0129] Bacterial species: Staphylococcus oralis

[0130] -: No bacterial growth observed +: Bacterial growth observed

[0131] Bacterial species: Staphylococcus mitis

[0132] -: No bacterial growth observed +: Bacterial growth observed

[0133] Bacterial species: Porphyromonas gingivalis

[0134] -: No bacterial growth observed +: Bacterial growth observed

[0135] Bacterial species: Staphylococcus gordonii

[0136] -: No bacterial growth observed +: Bacterial growth observed

[0137] Bacterial species: Enterococcus faecalis

[0138] -: No bacterial growth observed +: Bacterial growth observed

[0139] Bacterial species: Lactobacillus casei

[0140] -: No bacterial growth observed +: Bacterial growth observed

[0141] <Selective antimicrobial and / or bactericidal test 2> (1) Test substance: Ozone-treated glycerin (OG2000) - Target substance: Concentrated glycerin solution (glycerin with a concentration of 98% by mass or higher of the Japanese Pharmacopoeia product)

[0142] (2) Test bacterial strains - Enterococcus faecalis (JCM5803) - Lactobacillus casei (JCM1134) - Streptococcus thermophilus (JCM20026)・Bifidobacterium longum subsp infantis (JCM1222)

[0143] (3) Test materials 1) Dilution solution for the stock sample: 50% glycerin aqueous solution, sterile water, sterile PBS (phosphate-buffered saline), reaction stop solution (0.02 mol / L sodium thiosulfate)

[0144] 2) Culture medium E. faecalis (i) Culture medium for bacterial suspension preparation: Brainheart Infusion (BHI) agar medium 52 g of Brainheart Infusion Agar (BD) was dissolved in 1 L of distilled water and autoclaved at 121°C for 15 minutes. The mixture was dispensed into 20 mL portions into petri dishes, solidified, and stored at 4°C until use. (ii) Liquid culture medium for post-sensitization: Brainheart Infusion (BHI) liquid medium 37 g of Brainheart Infusion Broth (BD) was dissolved in 1 L of distilled water and autoclaved at 121°C for 15 minutes. (iii) Confirmation agar medium: Brainheart Infusion (BHI) agar medium The same BHI agar medium prepared in (i) above was used.

[0145] L. casei, S. thermophilus (i) Culture medium for bacterial suspension preparation: MRS agar medium 62 g of MRS agar (Oxoid) was dissolved in 1 L of distilled water and autoclaved at 121°C for 15 minutes. The mixture was dispensed into 20 mL portions into petri dishes, solidified, and stored at 4°C until use. (ii) Liquid medium for pre-culture: MRS liquid medium 52.2 g of MRS broth (Millipore) was dissolved in 1 L of distilled water and autoclaved at 121°C for 15 minutes. (iii) Confirmation agar medium: MRS agar medium The same MRS agar medium prepared in (i) above was used.

[0146] B. longum subsp. infantis (i) Culture medium for bacterial suspension preparation: BL agar medium 60 g of BL agar (manufactured by Eiken Chemical Co., Ltd.) was dissolved in 950 mL of distilled water and autoclaved at 121 °C for 15 minutes. 50 mL of horse fibrin-free blood was added to the medium, which had been kept warm at approximately 50 °C, and mixed well. The mixture was dispensed into 20 mL portions into petri dishes, allowed to solidify, and stored at 4 °C under anaerobic conditions until use. (ii) Liquid culture medium for post-sensitization: L-cysteine-added MRS liquid medium 52.2 g of MRS broth (Millipore) was dissolved in 950 mL of distilled water and autoclaved at 121 °C for 15 minutes. 50 mL of filtration-sterilized 1% L-cysteine ​​solution was added to adjust the final concentration to 0.05%. (iii) Confirmation agar medium: BL agar medium The same BL agar medium prepared in (i) above was used.

[0147] 3) Sample Preparation Ozone-treated glycerin (OG2000) was diluted with sterile water to an oxidizing power of 1,000 ppm based on ozone concentration, and then diluted with 50% glycerin aqueous solution to achieve oxidizing powers of 500 ppm, 250 ppm, 100 ppm, 10 ppm, and 1 ppm.

[0148] 4) Preparation of bacterial suspensions E. faecalis, L. casei, and S. thermophilus were cultured in a bacterial suspension preparation medium at 37°C for 24 hours. B. longum subsp. infantis was cultured anaerobically in a bacterial suspension preparation medium at 37°C for 72 hours. The cultured bacteria were then prepared using sterile PBS to a McFarland ratio of 0.5 to create the inoculum.

[0149] 5) Antibacterial and / or bactericidal efficacy test (i) Dispense 0.9 mL each of the ozone-treated glycerin concentrations prepared in 3.3) into sterile small test tubes. (ii) Add 0.1 mL each of the bacterial suspensions prepared in 3.4) to the small test tubes containing ozone-treated glycerin. (iii) Sensitize the bacterial suspensions for (1 minute, 5 minutes, 10 minutes, 60 minutes, or 120 minutes). (iv) After sensitization, transfer the entire volume of the sensitized solution from 5) (iii) to a small test tube containing 20 μL of 0.02 mol / L sodium thiosulfate. (v) Transfer 0.1 mL of the sensitized solution from (iv) above to a small test tube containing 0.9 mL of liquid medium for post-sensitization culture or liquid medium for pre-culture, according to the bacterial species. The pre-culture conditions for each bacterial species were as follows: ・E. faecalis 37°C, 24 hours, aerobic culture; L. casei 37°C, 24 hours, aerobic culture; S. thermophilus 37°C, 24 hours, microaerobic culture; B. longum subsp. infantis 37°C, 48 hours, anaerobic culture. (v) After pre-culture, the viability of each bacterial species was confirmed using confirmation agar medium appropriate for each bacterial species.

[0150] (4) Test Results The antibacterial and / or bactericidal effects of ozone-treated glycerin against each bacterium are shown in Tables 7 to 10. For E. faecalis, bacterial growth was observed at all oxidizing powers (1,000 ppm, 500 ppm, 250 ppm, 100 ppm, 10 ppm, 1 ppm) for sensitization times of 1 to 120 minutes. For L. casei, oxidizing power of 100 ppm or higher was observed for sensitization times of 1 to 120 minutes. For S. thermophilus, oxidizing power of 500 ppm or higher was observed for sensitization times of 1 minute, 5 minutes, and 10 minutes, and oxidizing power of 100 ppm or higher was observed for sensitization times of 60 minutes and 120 minutes. Longum subsp. infantis showed an oxidizing power of 1 ppm or more after a sensitization time of 1 to 120 minutes.

[0151] Bacterial species: Enterococcus faecalis

[0152] -: No bacterial growth observed +: Bacterial growth observed

[0153] Bacterial species: Lactobacillus casei

[0154] -: No bacterial growth observed +: Bacterial growth observed

[0155] Bacterial species: Streptococcus thermophilus

[0156] -: No bacterial growth observed +: Bacterial growth observed

[0157] Bacterial species: Bifidobacterium longum subsp infantis

[0158] -: No bacterial growth observed +: Bacterial growth observed

[0159] <Antibacterial and / or bactericidal test of Candida albicans> Ozone-treated glycerin (oxidizing power of 10 ppm or 100 ppm based on ozone concentration) was found to have a strong antibacterial and / or bactericidal effect against Candida albicans, as shown in Table 11 and Figure 3.

[0160] *1 x 10 －２ Below the detection limit

[0161] <Selective antibacterial and / or bactericidal agent 1> - Ozone-treated glycerin (OG200): 100% by mass

[0162] <Selective antibacterial and / or bactericidal composition 1> - Ozone-treated glycerin (OG200): 90% by mass - Polyethylene glycol with an average molecular weight of 4000: 5% by mass - Concentrated glycerin: 1% by mass - Purified water: remaining amount / total: 100% by mass

[0163] <Selective antibacterial and / or bactericidal composition 2> - Ozone-treated glycerin (OG100): 90% by mass - Polyethylene glycol with an average molecular weight of 4000: 5% by mass - Concentrated glycerin: 1% by mass - Purified water: remaining amount / total: 100% by mass

[0164] <Selective antibacterial and / or bactericidal composition 3> - Ozone-treated glycerin (OG50): 90% by mass - Polyethylene glycol with an average molecular weight of 4000: 5% by mass - Concentrated glycerin: 1% by mass - Purified water: remaining amount / total: 100% by mass

[0165] <Selective antibacterial and / or bactericidal agent 1 for periodontal pocket injection> - Ozone-treated glycerin (OG200): 100% by mass [Method of use] Fill an injection syringe with selective antibacterial and / or bactericidal agent 1 for periodontal pocket injection, and directly inject the selective antibacterial and / or bactericidal agent 1 into the periodontal pocket using the injection syringe.

[0166] <Selective antibacterial and / or bactericidal composition 1 for periodontal pocket injection> - Ozone-treated glycerin (OG200): 90% by mass - Polyethylene glycol with an average molecular weight of 4000: 1% by mass - Concentrated glycerin: 1% by mass - Other ingredients (magnesium chloride, hydroxyethylcellulose, ammonia alkyl methacrylate copolymer, triacetin): appropriate amount - Purified water: remaining amount - Total: 100% by mass [How to use] Fill an injection syringe with selective antibacterial and / or bactericidal composition 1 for periodontal pocket injection, and directly inject the selective antibacterial and / or bactericidal composition 1 into the periodontal pocket using the injection syringe.

[0167] <Selective antibacterial and / or bactericidal composition 2 for periodontal pocket injection> - Ozone-treated glycerin (OG100): 90% by mass - Polyethylene glycol with an average molecular weight of 4000: 1% by mass - Concentrated glycerin: 1% by mass - Other ingredients (magnesium chloride, hydroxyethylcellulose, ammonia alkyl methacrylate copolymer, triacetin): appropriate amount - Purified water: remaining amount - Total: 100% by mass [How to use] Fill an injection syringe with selective antibacterial and / or bactericidal composition 2 for periodontal pocket injection, and inject the selective antibacterial and / or bactericidal composition 2 directly into the periodontal pocket using the injection syringe.

[0168] <Selective antibacterial and / or bactericidal composition 3 for periodontal pocket injection> - Ozone-treated glycerin (OG50): 90% by mass - Polyethylene glycol with an average molecular weight of 4000: 1% by mass - Concentrated glycerin: 1% by mass - Other ingredients (magnesium chloride, hydroxyethylcellulose, ammonia alkyl methacrylate copolymer, triacetin): appropriate amount - Purified water: remaining amount - Total: 100% by mass [How to use] Fill an injection syringe with selective antibacterial and / or bactericidal composition 3 for periodontal pocket injection, and inject the selective antibacterial and / or bactericidal composition 3 directly into the periodontal pocket using the injection syringe.

[0169] <Examples of cosmetic formulations> The following are examples of cosmetic formulations containing the selective antibacterial and / or bactericidal agent 1 and selective antibacterial and / or bactericidal compositions 1 to 3 of the present invention.

[0170] [Formulation Example 1] - All-in-One Gel - Purified water: 75.0% by mass 1,3-Butylene glycol (BG): 10.0% by mass Glycerin: 5.0% by mass Diglycerin: 4.0% by mass Selective antimicrobial and / or bactericidal agent 1: 1.0% by mass Squalane: 1.0% by mass Carboxyvinyl polymer: 0.4% by mass Potassium hydroxide: 0.2% by mass Dipropylene glycol (DPG): 0.1% by mass Total: 100.0% by mass

[0171] [Formulation Example 2] - All-in-One Gel - Purified water: 75.0% by mass 1,3-Butylene glycol (BG): 10.0% by mass Glycerin: 5.0% by mass Diglycerin: 4.0% by mass Selective antibacterial and / or bactericidal composition 1: 1.0% by mass Squalane: 1.0% by mass Carboxyvinyl polymer: 0.4% by mass Potassium hydroxide: 0.2% by mass Dipropylene glycol (DPG): 0.1% by mass Total: 100.0% by mass

[0172] [Formulation Example 3] - All-in-One Gel - Purified water: 75.0% by mass 1,3-Butylene glycol (BG): 10.0% by mass Glycerin: 5.0% by mass Diglycerin: 4.0% by mass Selective antibacterial and / or bactericidal composition 2: 3.0% by mass Squalane: 1.0% by mass Carboxyvinyl polymer: 0.4% by mass Potassium hydroxide: 0.2% by mass Dipropylene glycol (DPG): 0.1% by mass Total: 100.0% by mass

[0173] [Formulation 4] - All-in-One Gel - Purified water: 75.0% by mass 1,3-Butylene glycol (BG): 10.0% by mass Glycerin: 5.0% by mass Diglycerin: 4.0% by mass Selective antibacterial and / or bactericidal composition 3: 5.0% by mass Squalane: 1.0% by mass Carboxyvinyl polymer: 0.4% by mass Potassium hydroxide: 0.2% by mass Dipropylene glycol (DPG): 0.1% by mass Total: 100.0% by mass

[0174] <Examples of formulations in food and beverages> The following are examples of formulations in food and beverages containing the selective antibacterial and / or bactericidal agent 1 and selective antibacterial and / or bactericidal compositions 1 to 3 of the present invention.

[0175] [Formulation Example 1] Tablets with the following composition were manufactured by a conventional method: • Selective antimicrobial and / or bactericidal agent 1: 3.0 mg • Dolomite (containing 20% ​​calcium and 10% magnesium): 83.4 mg • Casein phosphopeptide: 16.7 mg • Maltitol: 136.8 mg • Collagen: 12.7 mg • Sucrose fatty acid ester: 12.0 mg

[0176] [Formulation Example 2] Tablets having the following composition were manufactured by a conventional method: • Selective antimicrobial and / or bactericidal composition 1: 3.0 mg • Dolomite (containing 20% ​​calcium and 10% magnesium): 83.4 mg • Casein phosphopeptide: 16.7 mg • Maltitol: 136.8 mg • Collagen: 12.7 mg • Sucrose fatty acid ester: 12.0 mg

[0177] [Formulation Example 3] Tablets having the following composition were manufactured by a conventional method: • Selective antimicrobial and / or bactericidal composition 2: 5.0 mg • Dolomite (containing 20% ​​calcium and 10% magnesium): 83.4 mg • Casein phosphopeptide: 16.7 mg • Maltitol: 136.8 mg • Collagen: 12.7 mg • Sucrose fatty acid ester: 12.0 mg

[0178] [Formulation Example 4] Tablets having the following composition were manufactured by a conventional method: • Selective antimicrobial and / or bactericidal composition 3: 10.0 mg • Dolomite (containing 20% ​​calcium and 10% magnesium): 83.4 mg • Casein phosphopeptide: 16.7 mg • Maltitol: 136.8 mg • Collagen: 12.7 mg • Sucrose fatty acid ester: 12.0 mg

[0179] <Examples of formulations for oral compositions> The following are examples of formulations for oral compositions containing the selective antibacterial and / or bactericidal agent 1 and selective antibacterial and / or bactericidal compositions 1 to 3 of the present invention.

[0180] [Formulation Example 1] -Toothpaste- • Selective antibacterial and / or bactericidal agent 1 1.0% by mass • Anhydrous silicic acid 15.0% by mass • Propylene glycol 5.0% by mass • Xanthan gum 1.1% by mass • Sodium alginate 0.6% by mass • Sodium polyacrylate 0.3% by mass • Sodium lauroyl methyl taurate 0.4% by mass • Isopropylmethylphenol 0.1% by mass • Sodium fluoride 0.3% by mass • Methyl parahydroxybenzoate 0.2% by mass • Sodium saccharin 0.05% by mass • Fragrance 1.5% by mass • Appropriate amount of sodium citrate • Purified water (total remaining amount) 100.00% by mass

[0181] [Formulation Example 2] -Toothpaste- • Selective antibacterial and / or bactericidal composition 1 1.0% by mass • Anhydrous silicic acid 15.0% by mass • Propylene glycol 5.0% by mass • Xanthan gum 1.1% by mass • Sodium alginate 0.6% by mass • Sodium polyacrylate 0.3% by mass • Sodium lauroyl methyl taurate 0.4% by mass • Isopropylmethylphenol 0.1% by mass • Sodium fluoride 0.3% by mass • Methyl parahydroxybenzoate 0.2% by mass • Sodium saccharin 0.05% by mass • Fragrance 1.5% by mass • Appropriate amount of sodium citrate • Purified water (total remaining amount) 100.00% by mass

[0182] [Formulation Example 3] -Toothpaste- • Selective antibacterial and / or bactericidal composition 2 1.5% by mass • Anhydrous silicic acid 15.0% by mass • Propylene glycol 5.0% by mass • Xanthan gum 1.1% by mass • Sodium alginate 0.6% by mass • Sodium polyacrylate 0.3% by mass • Sodium lauroyl methyl taurate 0.4% by mass • Isopropylmethylphenol 0.1% by mass • Sodium fluoride 0.3% by mass • Methyl parahydroxybenzoate 0.2% by mass • Sodium saccharin 0.05% by mass • Fragrance 1.5% by mass • Appropriate amount of sodium citrate • Purified water (total remaining amount) 100.00% by mass

[0183] [Formulation Example 4] -Toothpaste- • Selective antibacterial and / or bactericidal composition 3 5.0% by mass • Anhydrous silicic acid 15.0% by mass • Propylene glycol 5.0% by mass • Xanthan gum 1.1% by mass • Sodium alginate 0.6% by mass • Sodium polyacrylate 0.3% by mass • Sodium lauroyl methyl taurate 0.4% by mass • Isopropylmethylphenol 0.1% by mass • Sodium fluoride 0.3% by mass • Methyl parahydroxybenzoate 0.2% by mass • Sodium saccharin 0.05% by mass • Fragrance 1.5% by mass • Appropriate amount of sodium citrate • Purified water (total remaining amount) 100.00% by mass

Claims

1. A selective antimicrobial agent and / or selective microbiotaicide characterized by containing ozone-treated glycerin, having antimicrobial and / or microbiotacitative activity against harmful microorganisms, but not having antimicrobial and / or microbiotacitative activity against non-harmful microorganisms.

2. The selective antimicrobial agent and / or selective microbial agent according to claim 1, wherein the ozone-treated glycerin has an oxidizing power of 1 ppm to 2,000 ppm based on ozone concentration.

3. A selective antimicrobial composition and / or selective microbiotamic composition characterized by containing ozone-treated glycerin, having antimicrobial and / or microbiotamic activity against harmful microorganisms, but not having antimicrobial and / or microbiotamic activity against non-harmful microorganisms.

4. The selective antimicrobial composition and / or selective microbial composition according to claim 3, wherein the ozone-treated glycerin has an oxidizing power of 1 ppm to 2,000 ppm based on ozone concentration.

5. The selective antimicrobial composition and / or selective microbial composition according to claim 3, comprising polyethylene glycol (PEG) having an average molecular weight of 400 or more.

6. A selective antimicrobial composition and / or selective microbial composition for injection into periodontal pockets, wherein the composition according to any one of claims 3 to 5 is used by injecting it into the periodontal pocket.

7. The selective antimicrobial and / or selective microbial composition for injection into periodontal pockets according to claim 6, wherein the harmful microorganism is a periodontal disease bacterium, and the non-harmful microorganism is a commensal oral bacterium other than a periodontal disease bacterium.

8. A selective antimicrobial composition and / or selective microbial composition for intraoral application, wherein the composition according to any one of claims 3 to 5 is applied to the oral cavity.

9. The selective antimicrobial composition and / or selective microbial composition for intraoral application according to claim 8, wherein the harmful microorganisms are periodontal disease bacteria, caries-causing bacteria, or bad breath-causing bacteria, and the non-harmful microorganisms are non-pathogenic commensal bacteria in the oral cavity.

10. A selective antimicrobial composition and / or selective microbial composition for vaginal and vulvar application, wherein the composition according to any one of claims 3 to 5 is applied to the vagina and vulva.

11. The selective antimicrobial composition and / or selective microbial composition for vaginal and vulvar application according to claim 10, wherein the harmful microorganism is a Candida fungus and the non-harmful microorganism is a non-pathogenic commensal bacterium of the vagina and vulva.

12. A selective antimicrobial composition and / or selective microbial composition for improving the intestinal flora, which is used by taking or ingesting the composition according to any one of claims 3 to 5.

13. A cosmetic composition containing the composition described in any one of claims 3 to 5.

14. Food or beverage containing the composition described in any one of claims 3 to 5.

15. An oral composition containing the composition described in any one of claims 3 to 5.

16. A cleaning composition for animals containing the composition described in any one of claims 3 to 5.

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