Method for promoting efficient germination of seeds of valuable and rare endangered plant yunnanopilia longistaminea

Abstract

The invention discloses a method for promoting efficient germination of seeds of a valuable and rare endangered plant yunnanopilia longistaminea, wherein the method comprises the steps: collecting ripe yunnanopilia longistaminea seeds; removing seed coats, and breaking inhibition of the seed coats; carrying out cleaning and disinfection treatment on the seeds; carrying out root promoting culture, and soaking the seeds with a 50 mg / L GA3 solution for 24 h; soaking the seeds with a 100 mg / L GA3 solution for 24 h, placing the seeds at the temperature of 5 DEG C, and refrigerating for 7-14 days to change endogenous hormone change of the yunnanopilia longistaminea in seed germination and seedling building processes, so as to break epicotyl dormancy; placing the seeds in the environment of the temperature of 28 DEG C, and culturing; culturing for 3-4 weeks to obtain seedlings having euphylla grown; and providing the seedlings for wild transplanting after growing for 8-10 weeks. The system method directly adopting the yunnanopilia longistaminea seeds for fast and efficient cultivation of the seedlings effectively relieves the epicotyl dormancy of the yunnanopilia longistaminea, ensures that the germination rate of the yunnanopilia longistaminea seeds is more than or equal to 70%, effectively shortens the seedling culture time by 60 days, and is simple and convenient to operate, high in survival rate, and strong in practicability. An effective technical means is provided for species protection biology, restoration ecology, resource exploitation and utilization and the like.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for promoting efficient germination of rare and endangered beet tree seeds. Background technique

[0002] beet tree ( Yunnanopilia longistaminea ) belongs to the monotypic species of the genus Yunnanopilia of the family Opiliaceae, and is mainly distributed in the deciduous monsoon forests of river valleys at an altitude of 1000-1400m in Honghe Prefecture, Yunnan Province. It is also distributed in southeastern Yunnan and southwestern Guangxi, and is endemic to southwest my country plant. This species is a characteristic and precious wild woody vegetable plant in the Honghe River Basin of Yunnan Province. It has a long history of utilization. The local people call it "beet". "precious" food. Due to the large market demand and the main dependence on wild resources, resources have been overutilized and habitats have been severely damaged, resulting in a large reduction in wi...

Examples

example 1

[0036] (1) Collect seeds: collect mature beet tree fruits at the provenance, wash the fruit and peel of the beet tree with fine river sand, and obtain about 10 clean seeds;

[0037] (2) Break the inhibition of the seed coat: peel off the seed coat, leaving only the embryo and endosperm, and then disinfect the seeds: wash with washing powder water, dry the water, disinfect with 75% alcohol for 30 seconds, and then put in 0.1% mercuric chloride for 10 minutes, and finally washed with water 5-6 times, with 50ml / L GA 3 Seeds were soaked in the solution for 24 hours, and the surface moisture was blotted dry with filter paper. Use river sand to configure the root-inducing medium, that is, the above-mentioned sterilized river sand, and place the root-inducing medium in a plastic box of 60×40×30 cm for root-inducing. The specific method is: put a layer of small gravel about 1 cm thick in the plastic box as a filter layer, and then spread a layer of root promoting medium about 1 cm th...

example 2

[0041] Change the ratio of the germination substrate to loam: humus = 3:1, and the other cultivation methods and contents are the same as Example 1, and the survival rate of the obtained seedlings reaches 70%.

[0042] Materials and precautions not mentioned in the examples are as above.

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for promoting efficient germination of rare and endangered beet tree seeds. Background technique

[0002] beet tree ( Yunnanopilia longistaminea ) belongs to the monotypic species of the genus Yunnanopilia of the family Opiliaceae, and is mainly distributed in the deciduous monsoon forests of river valleys at an altitude of 1000-1400m in Honghe Prefecture, Yunnan Province. It is also distributed in southeastern Yunnan and southwestern Guangxi, and is endemic to southwest my country plant. This species is a characteristic and precious wild woody vegetable plant in the Honghe River Basin of Yunnan Province. It has a long history of utilization. The local people call it "beet". "precious" food. Due to the large market demand and the main dependence on wild resources, resources have been overutilized and habitats have been severely damaged, resulting in a large reduction in wi...

Examples

example 1

[0036] (1) Collect seeds: collect mature beet tree fruits at the provenance, wash the fruit and peel of the beet tree with fine river sand, and obtain about 10 clean seeds;

[0037] (2) Break the inhibition of the seed coat: peel off the seed coat, leaving only the embryo and endosperm, and then disinfect the seeds: wash with washing powder water, dry the water, disinfect with 75% alcohol for 30 seconds, and then put in 0.1% mercuric chloride for 10 minutes, and finally washed with water 5-6 times, with 50ml / L GA 3 Seeds were soaked in the solution for 24 hours, and the surface moisture was blotted dry with filter paper. Use river sand to configure the root-inducing medium, that is, the above-mentioned sterilized river sand, and place the root-inducing medium in a plastic box of 60×40×30 cm for root-inducing. The specific method is: put a layer of small gravel about 1 cm thick in the plastic box as a filter layer, and then spread a layer of root promoting medium about 1 cm th...

example 2

[0041] Change the ratio of the germination substrate to loam: humus = 3:1, and the other cultivation methods and contents are the same as Example 1, and the survival rate of the obtained seedlings reaches 70%.

[0042] Materials and precautions not mentioned in the examples are as above.