A method for producing functional ganoderma fermented feed

Abstract

The present invention provides a method for manufacturing a functional ganoderma lucidum fermented feed. The present invention takes high-quality utilization of renewable resources as the inventive point. The raw materials of the ganoderma lucidum fermented feed include ganoderma lucidum seed culture solution and solid fermentation medium. Through the reasonable combination of various raw materials, as well as ganoderma lucidum biological fermentation and conversion process, a ganoderma lucidum fermented feed with multiple functions is manufactured. The product of the present invention contains functional ingredients such as ganoderma lucidum polysaccharides, chitooligosaccharides, ganoderma acid, enzymatic small peptides, complex enzymes, etc. The present invention solves the effect of anti-nutritional factors in cereal raw materials in feed on feed utilization, and improves feed utilization. Experiments have shown that the ganoderma lucidum fermented feed produced by the present invention has the functions of preventing and resisting diseases and regulating intestinal flora.

Description

Technical Field

[0001] The present invention relates to the fields of bioengineering and feed processing, and more specifically to a method for manufacturing ganoderma fermented feed with health care function by applying ganoderma fermentation and bioconversion. Background Art

[0002] In recent years, my country's marine and freshwater aquaculture has developed rapidly, and shellfish farming such as shrimp and scallops has become quite common. Every year, tens of millions of tons of fish heads, fish skins, fish bones, scallops and shrimp shells are abandoned on the beach to rot and deteriorate, causing serious environmental pollution and waste of these renewable resources. On the other hand, protein and amino acid feed raw materials are in short supply and prices are rising. Orange peels and other fruit peels are another type of huge renewable resource in my country. They have been abandoned for a long time, causing harm to the environment.

[0003] In livestock and poultry farming, low feed utilization and high mortality are bottlenecks that restrict the benefits of the industry. From the perspective of regulating the immune capacity of livestock and poultry and eliminating anti-nutritional factors in feed, adding bioactive substances such as functional polysaccharides, small peptides, oligosaccharides, terpenes and saponins to daily feed configuration can significantly improve disease resistance and feed utilization. Summary of the invention

[0004] The purpose of the present invention is to provide a new method for producing ganoderma fermented feed by effectively utilizing renewable resources.

[0005] The present invention comprehensively solves the above technical problems from two aspects: formulation and process. The technical solution adopted by the present invention is:

[0006] A method for preparing a functional ganoderma fermented feed comprises the following steps:

[0007] (a) adding a certain proportion of aquatic product processing waste powder, orange peel powder and / or orange peel powder, corn flour, soybean meal and bran, and stirring evenly with cold water, and then heating the stirred product to obtain a seed culture medium solution;

[0008] (b) cooling the seed culture medium solution, inoculating Ganoderma lucidum, and culturing at a certain speed and temperature to obtain Ganoderma lucidum seed culture solution;

[0009] (c) mixing the aquatic product processing waste powder, orange peel powder and / or orange peel powder, corn flour, soybean meal and bran in proportion to obtain a solid fermentation material; and mixing the solid fermentation material with water in proportion to obtain a solid fermentation medium;

[0010] (d) inoculation and fermentation: mixing the Ganoderma lucidum seed culture solution obtained in step (b) with the solid fermentation medium obtained in step (c) in proportion, and placing the mixture into a fermentation tank or fermentation tank for fermentation to obtain Ganoderma lucidum fermentation material;

[0011] (e) heating the fermented Ganoderma lucidum material obtained in step (d) and performing heat preservation treatment to obtain a complete fermented Ganoderma lucidum feed;

[0012] (f) Processing the complete fermented Ganoderma lucidum feed described in (e) by conventional techniques to obtain powdered or granular fermented Ganoderma lucidum feed.

[0013] Preferably, in step (a), the weight proportions of the components of the seed culture medium solution are: 0.1-5 parts of aquatic product processing waste powder, 0.1-3 parts of orange peel powder and / or orange peel powder, 0.5-10 parts of corn flour, 0.5-10 parts of soybean meal, 0.1-3 parts of bran, and 50-85 parts of cold water; the cold water temperature is 10°C-50°C.

[0014] Preferably, in step (a), the stirred material is heated and sterilized at 121° C. for 30 minutes to obtain a seed culture medium solution.

[0015] Preferably: the seed culture medium is cooled to 30° C., inoculated with 0.5-15 parts by weight of Ganoderma lucidum, and cultured at a rotation speed of 100-400 rpm and a culture temperature of 10° C.-40° C. for 3-10 days to obtain Ganoderma lucidum seed culture solution.

[0016] Preferably: the weight proportions of the components of the solid-state fermentation material are: 10-30 parts of aquatic product processing waste powder, 5-20 parts of tangerine peel powder and / or orange peel powder, 30-60 parts of corn flour, 10-30 parts of soybean meal, and 10-60 parts of rice bran; the solid-state fermentation material is evenly mixed with 10℃-60℃ water in a weight ratio of 1:1-1.2 to obtain a solid-state fermentation medium.

[0017] Preferably, the Ganoderma lucidum seed culture solution and the solid fermentation medium are mixed in a ratio of 1-20 parts by weight, the fermentation temperature is 10° C.-40° C., and the fermentation time is 3 days-20 days.

[0018] Preferably, the fermented Ganoderma lucidum feed is heated to 30°C-80°C and kept warm for 1 hour-10 hours to obtain a complete fermented Ganoderma lucidum feed.

[0019] Preferably, the aquatic product processing waste materials are shrimp shells and / or shells and / or fish bones from fresh water and sea water.

[0020] A functional ganoderma fermented feed, the raw materials of which include ganoderma seed culture solution and solid fermentation medium; wherein the ganoderma seed culture solution is composed of aquatic product processing waste powder, tangerine peel powder and / or orange peel powder, corn flour, soybean meal, bran, water and ganoderma; the solid fermentation medium is composed of aquatic product processing waste powder, tangerine peel powder and / or orange peel powder, corn flour, soybean meal, bran and water.

[0021] The weight proportions of the components in the ganoderma lucidum seed culture solution are: 0.1-5 parts of aquatic product processing waste powder, 0.1-3 parts of orange peel powder and / or orange peel powder, 0.5-10 parts of corn flour, 0.5-10 parts of soybean meal, 0.1-3 parts of bran, 50-85 parts of water, and 0.5-15 parts of ganoderma lucidum.

[0022] The solid-state fermentation medium is composed of solid-state fermentation material and water; wherein the weight proportions of the components of the solid-state fermentation material are: 10-30 parts of aquatic product processing waste powder, 5-20 parts of orange peel powder and / or orange peel powder, 30-60 parts of corn flour, 10-30 parts of soybean meal, and 10-60 parts of rice bran; and the weight ratio of the solid-state fermentation material to water is 1:1-1.2.

[0023] The excellent effects of the present invention are:

[0024] The present invention selects cheap marine waste and fruit product waste, uses bioconversion technology to turn them into treasure, uses edible and medicinal fungus Ganoderma lucidum as a fermentation agent, and realizes efficient production and utilization of bioactive substances such as functional polysaccharides, small peptides, oligosaccharides, terpenes and saponins through deep fermentation and bioconversion. The present invention uses bioconversion technology to turn them into treasure, the process route and conditions, the unique combination of conventional materials + shrimp shell powder + orange peel powder and / or orange peel powder, deep fermentation and enzyme bioconversion of Ganoderma lucidum, and the final temperature increase and heat preservation process ensure the formation of functional Ganoderma lucidum fermented feed, while solving the problem of feed anti-nutritional factors. The mass percentage of each functional component in the fermented ganoderma feed of the present invention is as follows: ganoderma polysaccharide 1-10%, chitosan oligosaccharide 0.1-3%, ganoderma acid 0.1-1%, enzyme-modified small peptide (molecular weight below 500 Dalton) 0.5-5%, hesperidin 0.05-1.5%; the activity of chitosan degradation enzyme contained in the complex enzyme is greater than 20 IU / g. Feeding experiments have proved that the present invention can prevent and resist diseases, regulate intestinal flora, improve feed utilization, and improve meat quality. DETAILED DESCRIPTION

[0025] After extensive and in-depth research, the inventors have for the first time used a combination of deep-fermented Ganoderma lucidum shrimp shell powder and shell powder + orange peel powder and / or orange peel powder + daily feed to produce a stable, functional, complete Ganoderma lucidum fermented feed, which solves the problems of feed utilization and survival rate of livestock and poultry farming and improves the meat quality of livestock and poultry.

[0026] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or under conditions recommended by the manufacturer.

[0027] Example 1-Seed culture medium preparation

[0028] Add 1 part of shrimp shell powder, 3.5 parts of corn flour, 0.5 parts of soybean meal, 1.3 parts of rice bran, and 0.7 parts of tangerine peel powder and / or orange peel powder to cold water and stir evenly, then heat, sterilize at 121°C for 30 minutes, cool to 30°C, inoculate 5 parts of Ganoderma lucidum under sterile conditions, and culture at a speed of 200 rpm and a culture temperature of 30°C for 7 days to obtain Ganoderma lucidum seed culture solution; after inspection, the biomass is greater than 1.35 parts.

[0029] Example 2-Ganoderma lucidum fermentation 1

[0030] 40 parts of corn flour, 10 parts of soybean meal, 35 parts of rice bran, 10 parts of shrimp shell powder, and 5 parts of orange peel powder were mixed evenly to obtain a solid fermentation material, and the solid fermentation material was mixed evenly with 50°C water in a ratio of 1:1 to obtain a solid fermentation medium; 10 parts of the inoculation amount of Ganoderma lucidum seed liquid was added, mixed and loaded into a fermentation tank, and fermented at 25°C for 14 days to obtain Ganoderma lucidum fermentation material; then the temperature was raised to 45°C and kept warm for 5 hours to obtain a full-price Ganoderma lucidum fermentation feed; after drying at 90°C and crushing, a granular Ganoderma lucidum fermentation feed was obtained. After inspection, the weight portions of each functional component are shown in Table 1.

[0031] Table 1 Weight portions of effective ingredients in Ganoderma lucidum fermented feed (parts)

[0032] Ganoderma lucidum polysaccharide Chitooligosaccharides Ganoderic acid Enzymatic small peptide Hesperidin Chitosan degrading enzyme Before insulation 1.49 0.36 0.29 0.84 0.11 28IU After insulation 1.58 0.65 0.32 1.21 0.15 22IU

[0033] Example 3-Ganoderma lucidum fermentation 2

[0034] 35 parts of corn flour, 15 parts of soybean meal, 25 parts of bran, 15 parts of shrimp shell powder, and 10 parts of orange peel powder were mixed evenly to obtain solid fermentation material, and the solid fermentation material was mixed evenly with 80°C water at a ratio of 1:1.2 to obtain solid fermentation medium; 15 parts of the inoculation amount of Ganoderma lucidum seed liquid was added, mixed and loaded into a fermentation tank, and fermented at 30°C for 10 days to obtain Ganoderma lucidum fermentation material; then the temperature was raised to 55°C and kept warm for 8 hours to obtain full-price Ganoderma lucidum fermentation feed; after drying at 90°C and crushing, granular Ganoderma lucidum fermentation feed was obtained. After inspection, the weight portions of each functional component are shown in Table 2.

[0035] Table 2 Weight portions of effective ingredients in Ganoderma lucidum fermented feed (parts)

[0036] Ganoderma lucidum polysaccharide Chitooligosaccharides Ganoderic acid Enzymatic small peptide Hesperidin Chitosan degrading enzyme Before insulation 1.55 0.42 0.32 1.16 0.25 30IU / g After insulation 1.69 1.58 0.45 2.08 0.53 19IU / g

[0037] Example 4 - Duck farming

[0038] Cherry Valley ducks aged 25 days were selected and divided into two groups according to the diet group (control group) and the Ganoderma lucidum fermented feed group (experimental group), with 3 replicates in each group and 10 ducks in each replicate. The feeding method and management were carried out according to the routine. After 25 days of feeding, all indicators were better than those of the control group, as shown in Table 3.

[0039] Table 3 Experimental results of feeding meat ducks with fermented Ganoderma lucidum feed

[0040]

[0041] Example 5 - Chicken farming

[0042] 21-day-old large yellow-feathered broilers were selected and divided into two groups according to the diet group (control group) and the Ganoderma lucidum fermented feed group (experimental group), with 3 replicates in each group and 10 chickens in each replicate. The feeding method and management were carried out according to the routine. After 25 days of feeding, all indicators were better than those of the control group, as shown in Table 4.

[0043] Table 4 Experimental results of feeding broiler chickens with fermented Ganoderma lucidum feed

[0044]

[0045] Example 6 - Pig farming

[0046] 60 30-day-old weaned three-way hybrid piglets were selected and divided into two groups according to the diet group (control group) and the Ganoderma lucidum fermented feed group (experimental group), with 3 replicates in each group and 10 pigs in each replicate. The feeding method and management were carried out according to the routine. After 15 days of feeding, all indicators were better than those of the control group, as shown in Table 5.

[0047] Table 5 Experimental results of feeding piglets with fermented Ganoderma lucidum feed

[0048] Feed to meat ratio Survival rate Intestinal lactobacilli count (Log10cfu / g feces Control group 1.45±0.1 4.5 5.64 Experimental Group 1.32±0.1 1.3 7.91

[0049] It should be understood that within the scope of the present invention, the above-mentioned technical features of the present invention can be combined with each other to form a new or preferred technical solution. Any modification or equivalent substitution made by ordinary technicians in the relevant field to the specific implementation of the present invention without departing from the spirit and scope of the present invention is within the scope of protection of the claims of the present invention.

Claims

1. A method for preparing a functional Ganoderma lucidum fermented feed, characterized in that: Includes steps: (a) adding a certain proportion of aquatic product processing waste powder, orange peel powder and / or orange peel powder, corn flour, soybean meal and bran, and stirring evenly with cold water, and then heating the stirred product to obtain a seed culture medium solution; (b) cooling the seed culture medium solution, inoculating Ganoderma lucidum, and culturing at a certain speed and temperature to obtain Ganoderma lucidum seed culture solution; (c) mixing the aquatic product processing waste powder, orange peel powder and / or orange peel powder, corn flour, soybean meal and bran in proportion to obtain a solid fermentation material; and mixing the solid fermentation material with water in proportion to obtain a solid fermentation medium; (d) inoculation and fermentation: mixing the Ganoderma lucidum seed culture solution obtained in step (b) with the solid fermentation medium obtained in step (c) in proportion, and placing the mixture into a fermentation tank or fermentation tank for fermentation to obtain Ganoderma lucidum fermentation material; In step (d), the ganoderma lucidum seed culture solution and the solid fermentation medium are mixed in a ratio of 1-20 parts by weight, the fermentation temperature is 25° C.-30° C., and the fermentation time is 3 days-20 days; (e) heating the fermented Ganoderma lucidum material obtained in step (d) to 45° C.-55° C. and heat-treating the material for 1 hour-10 hours to obtain a complete fermented Ganoderma lucidum feed; and (f) processing the complete Ganoderma lucidum fermented feed described in step (e) to obtain powdered or granular Ganoderma lucidum fermented feed; Wherein, in step (b), the weight parts of the components in the ganoderma lucidum seed culture solution are: 0.1-5 parts of aquatic product processing waste powder, 0.1-3 parts of orange peel powder and / or orange peel powder, 0.5-10 parts of corn flour, 0.5-10 parts of soybean meal, 0.1-3 parts of bran, 50-85 parts of water, and 0.5-15 parts of ganoderma lucidum; and In step (c), the solid-state fermentation medium is composed of solid-state fermentation material and water; wherein the weight parts of the components of the solid-state fermentation material are: 10-30 parts of aquatic product processing waste powder, 5-20 parts of orange peel powder and / or orange peel powder, 30-60 parts of corn flour, 10-30 parts of soybean meal, and 10-60 parts of bran; the weight ratio of the solid-state fermentation material to water is 1:1-1.2; The aquatic product processing waste materials are shrimp shells from fresh water and sea water.

2. The method for preparing the functional Ganoderma lucidum fermented feed according to claim 1, characterized in that: In step (a), the temperature of the cold water is 10°C-50°C.

3. The method for preparing the functional Ganoderma lucidum fermented feed according to claim 1, characterized in that: In step (a), the stirred material is heated and sterilized at 121° C. for 30 minutes to obtain a seed culture medium solution.

4. The method for preparing the functional Ganoderma lucidum fermented feed according to claim 1, characterized in that: In step (b), the seed culture medium is cooled to 30° C., inoculated with 0.5-15 parts by weight of Ganoderma lucidum, and cultured at a rotation speed of 100-400 rpm and a culture temperature of 10° C.-40° C. for 3-10 days to obtain Ganoderma lucidum seed culture solution.

5. The method for preparing the functional Ganoderma lucidum fermented feed according to claim 1, characterized in that: In step (e), the temperature of the fermented Ganoderma lucidum feed is raised to 55° C. and kept warm for 1 hour to 10 hours to obtain a complete fermented Ganoderma lucidum feed.

6. A functional Ganoderma lucidum fermented feed, characterized in that: The Ganoderma lucidum fermented feed is prepared by the preparation method according to any one of claims 1 to 5.

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