Egg rich in DHA nutrient and production method thereof

By adding specific raw materials to laying hen feed and using composite DHA microcapsules, the problem of low DHA content in ordinary eggs is solved, effective enrichment of DHA in eggs and the increase in laying hen egg laying rate is achieved, and the oxidative rancidity of DHA is reduced.

CN119867010AInactive Publication Date: 2025-04-25QINGDAO ZAOYUAN MARINE BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202411990253.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-31
Publication Date
2025-04-25
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

In the prior art, the DHA content in ordinary eggs is low, and it is difficult to achieve the purpose of supplementing lecithin-type DHA by eating ordinary egg yolks. At the same time, the addition of flaxseed oil to the feed will increase the burden on the laying hens' liver, resulting in fatty liver and affecting the egg laying rate.

Method used

By adding corn flour, fermented soybean meal, shell powder, sea buckthorn seed meal and corn oil to the laying hen feed, and using composite DHA microcapsules, including algae oil, fish oil, and krill oil as DHA sources, the physiological efficacy time of DHA is extended through wall embedding of chitosan, β-cyclodextrin, sodium alginate and corn cob powder, gelatin, xanthan gum, and glycerin.

Benefits of technology

The effective enrichment of DHA in eggs is achieved, the content of DHA in egg yolk is increased, the digestion and absorption of nutrients by laying hens is promoted, the utilization rate of feed and the laying hens are improved, and the oxidative rancidity of DHA is reduced.

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Abstract

The invention discloses an egg rich in DHA nutrient and a production method of the egg. The production method of the eggs rich in the DHA nutrient comprises the following steps: during the egg laying period of laying hens, feeding the laying hens with a laying hen feed continuously for more than 15 days to obtain the eggs rich in the DHA nutrient, the feed comprises corn flour, fermented soybean meal, shell powder, seabuckthorn seed meal, a premix and corn oil. The premix is prepared from the following raw materials in parts by weight: 40 to 50 parts of puffed corn flour, 30 to 40 parts of compound DHA (docosahexaenoic acid) microcapsules, 2 to 4 parts of krill meal, 2 to 4 parts of sea cucumber freeze-dried powder, 2 to 5 parts of cotton seed oligosaccharide, 0.5 to 2 parts of DL-methionine, 0.2 to 2 parts of trace element premix, 0.02 to 0.04 part of vitamin A acetate and 0.2 to 0.4 part of vitamin D3. The DHA content of the egg yolk of the egg produced by the method disclosed by the invention can be more than 250mkg / 100kg, and the enrichment of the DHA of the egg yolk is effectively realized.
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Description

Technical Field

[0001] The present application relates to the technical field of egg production, and more specifically, to an egg rich in DHA nutrients and a production method thereof. Background Art

[0002] DHA, or docosahexaenoic acid, is a polyunsaturated fatty acid essential to the human body. It plays an important role in assisting brain cell development, anti-aging, improving blood circulation, and lowering blood lipids. Since DHA is difficult to synthesize directly in the human body, it needs to be obtained from foods rich in DHA, such as deep-sea fish, nuts, and algae.

[0003] DHA is divided into fish oil DHA, algae oil DHA and egg yolk DHA based on its source. DHA from fish oil and algae oil is triglyceride type, methyl ester type or ethyl ester type, and DHA from egg yolk is lecithin type. The structural difference or existence state of DHA will have an important impact on its digestion and absorption in the human body; among them, the absorption rate of ethyl ester DHA is only about 20%, the absorption rate of triglyceride type and methyl ester type is about 50%, and the absorption rate of lecithin type DHA can reach more than 90%.

[0004] Since lecithin DHA only exists in egg yolk, it can only be obtained through egg yolk. However, at present, ordinary eggs contain about 30-40mkg DHA per 100kg whole eggs. The DHA content is low, and it is difficult to supplement lecithin DHA by eating ordinary egg yolk. Therefore, in order to increase the DHA in poultry eggs, the related technology adds raw materials such as fish oil, linseed oil, algae oil, etc. to the feed of laying hens to obtain eggs rich in DHA nutrients.

[0005] However, the fish oil and algae oil in feed are mostly crude fish oil or crude algae oil, which are easily affected by external conditions such as temperature and light, causing oxidation and rancidity, making the DHA in them lose their physiological effects. Flaxseed oil is the vegetable oil with the highest content of α-linolenic acid, and its efficiency of conversion into DHA in laying hens is relatively high. However, adding flaxseed oil to feed will increase the burden on the liver of laying hens, leading to the production of fatty liver and affecting the egg production rate of laying hens. Summary of the invention

[0006] In order to solve the above technical problems, the present application provides an egg rich in DHA nutrients and a production method thereof.

[0007] In a first aspect, the present application provides a method for producing eggs rich in DHA nutrients, using the following technical solution: A method for producing eggs rich in DHA nutrients, wherein during the egg-laying period, laying hens are fed with laying hen feed for more than 15 consecutive days to obtain eggs rich in DHA nutrients; The feed comprises the following raw materials in parts by weight: 200-220 parts of corn flour, 80-100 parts of fermented soybean meal, 20-30 parts of shell powder, 10-20 parts of sea buckthorn seed meal, 3-5 parts of premix and 4-6 parts of corn oil; The premix comprises the following raw materials in parts by weight: 40-50 parts of puffed corn flour, 30-40 parts of composite DHA microcapsules, 2-4 parts of krill powder, 2-4 parts of freeze-dried sea cucumber powder, 2-5 parts of cottonseed oligosaccharides, 0.5-2 parts of DL-methionine, 0.2-2 parts of trace element premix, 0.02-0.04 parts of vitamin A acetate and 0.2-0.4 parts of vitamin D3.

[0008] By adopting the above technical scheme, during the laying period, by feeding the feed rich in DHA nutrients of the present application, effective enrichment of DHA in eggs can be achieved, thereby increasing the content of DHA in egg yolks. In addition, in addition to corn flour as the main raw material, the feed also contains fermented soybean meal, shell powder, sea buckthorn seed meal and corn oil, which can promote the digestion and absorption of nutrients in the feed by laying hens and effectively improve feed utilization.

[0009] The premix of the present application is added with composite DHA microcapsules from multiple DHA sources, and through the compounding of krill powder and sea cucumber freeze-dried powder, DHA from different sources can be provided, so as to provide laying hens with richer nutrients; through the addition of puffed corn flour and cottonseed oligosaccharides, the feed intake of laying hens can be increased, the feed utilization rate can be improved, the synthesis and metabolism of nutrients can be promoted, the egg production rate and egg-laying cycle of laying hens can be increased, and the gastrointestinal flora of laying hens can be improved.

[0010] In addition, in order to reduce the oxidative rancidity of DHA and improve the bioavailability of DHA, DHA is made into microcapsules to avoid direct contact between DHA and ambient air, which is beneficial to prolong the physiological efficacy time of DHA.

[0011] Optionally, the composite DHA microcapsule comprises a core material, a first wall material embedding the core material, and a second wall material embedding the first wall material; The core material comprises the following raw materials in parts by weight: 20-30 parts of algae oil, 10-15 parts of fish oil, 1-3 parts of krill oil, 0.02-0.06 parts of antioxidant, 0.05-0.08 parts of emulsifier; The first wall material comprises chitosan, β-cyclodextrin and sodium alginate in a weight ratio of 3-5:2-4:1; The second wall material comprises corn cob powder, gelatin, xanthan gum and glycerin in a weight ratio of 10-12:5-7:3-5:1.

[0012] By adopting the above technical scheme, using algae oil, fish oil, and krill oil as the source of DHA, and mixing them with antioxidants and emulsifiers as the core material, the physiological effects of DHA caused by fluctuations in external conditions can be effectively avoided. In addition, since the intestines of chickens are relatively short and their digestive capacity is limited, if DHA is fed directly, DHA may be excreted from the body before being absorbed and utilized by the chickens, affecting their enrichment of DHA. Therefore, the first wall material composed of chitosan, β-cyclodextrin, and sodium alginate can have a good embedding effect on the core material; the second wall material composed of corn cob powder, gelatin, xanthan gum, and glycerol can effectively prolong the residence time of the composite DHA microcapsule in the intestines and stomachs of laying hens to improve the bioavailability of DHA in laying hens.

[0013] Optionally, the composite antioxidant comprises tea polyphenol palmitate, curcumin, L-ascorbyl palmitate, and vitamin E in a weight ratio of 3-5:2-4:1-3:1-3.

[0014] By adopting the above technical solution, tea polyphenol palmitate, curcumin, L-ascorbyl palmitate, and vitamin E are all fat-soluble food antioxidants, which makes them have a good antioxidant effect on algae oil, fish oil, and krill oil to improve the stability of DHA therein. In addition, curcumin, as a natural compound, also has good anti-inflammatory properties to improve the disease resistance of laying hens.

[0015] Optionally, the emulsifier comprises lactic acid fatty acid glyceride and gum arabic in a weight ratio of 2-4:1.

[0016] By adopting the above technical scheme, the emulsifier composed of lactic acid fatty acid glyceride and gum arabic has good emulsification stability for algae oil, fish oil and krill oil.

[0017] Optionally, the composite DHA microcapsules are prepared by the following method: Mixing the algae oil, the fish oil, the krill oil, the antioxidant and the emulsifier to obtain the core material; Dissolving the chitosan, the β-cyclodextrin and the sodium alginate in water to obtain a first wall material solution with a mass fraction of 10-15%; Dissolving the corncob powder, the gelatin, the xanthan gum, and the glycerin in water, and heating to 80-90° C. to obtain a second wall material solution with a mass fraction of 5-10 wt %; The first wall material solution is mixed with the core material, and emulsified by high-speed stirring to obtain a first emulsion; mixing the second emulsion with the second wall material solution to obtain a second emulsion; The second emulsion is homogenized and spray-dried to obtain the composite DHA microcapsules.

[0018] Optionally, the high-speed stirring emulsification includes: stirring for 10-20 minutes at a rotation speed of 8000-10000 rpm.

[0019] Optionally, the homogenization pressure is 10-20 MPa, and the homogenization times are 1-3 times.

[0020] Optionally, the inlet air temperature of the spray drying is 170-180°C, the outlet air temperature is 70-80°C, the feed temperature is 25-40°C, and the atomization pressure is 0.6-0.8MPa.

[0021] In a second aspect, the present application provides an egg rich in DHA nutrients, produced by the production method as described above.

[0022] In summary, this application has the following beneficial effects: Through composite DHA microcapsules from multiple sources of DHA, and through the compounding of krill powder and sea cucumber freeze-dried powder, DHA from different sources can be provided to provide laying hens with richer nutrients; the oil containing DHA is made into microcapsules to avoid direct contact between DHA oil and air, which is conducive to prolonging the physiological effect of DHA. During the egg-laying period of laying hens, by feeding the feed rich in DHA nutrients of this application, effective enrichment of DHA in eggs can be achieved, thereby increasing the content of DHA in egg yolks. In addition, in addition to corn flour as the main raw material, fermented soybean meal, shell powder, sea buckthorn seed meal and corn oil are added to the feed, which can promote the digestion and absorption of nutrients in the feed by laying hens, and effectively improve feed utilization. DETAILED DESCRIPTION

[0023] The present application is further described in detail below in conjunction with embodiments.

[0024] It should be noted that, unless otherwise specified, the raw materials in the following examples are all commercially available.

[0025] The composition of the trace element premix is: FeSO4 100kg, ZnSO4·H2O 15kg, CuSO4·5H2O 100kg, iodine 0.8kg, MnSO4 100kg, Na2SeO3 0.2kg.

[0026] Preparation Example Preparation Example 1 of Composite DHA Microcapsules ① Mix 20kg of algae oil, 10kg of fish oil, 1kg of krill oil, 0.02kg of antioxidant and 0.05kg of emulsifier to obtain a core material; wherein the antioxidant includes tea polyphenol palmitate, curcumin, L-ascorbyl palmitate, and vitamin E in a weight ratio of 3:2:1:1; the emulsifier includes lactic acid fatty acid glyceride and gum arabic in a weight ratio of 2:1.

[0027] ② Dissolve chitosan, β-cyclodextrin and sodium alginate in water at a weight ratio of 3:2:1 to obtain a first wall material solution with a mass fraction of 10-15%; ③ Dissolve corncob powder, gelatin, xanthan gum and glycerin in a weight ratio of 10:5:3:1 in water, heat to 80°C, and obtain a second wall material solution with a mass fraction of 5wt%; ④ Mixing the first wall material solution and the core material at a weight ratio of 20:1, stirring and emulsifying at a high speed of 8000 rpm for 20 minutes to obtain a first emulsion; ⑤ Mixing the second emulsion and the second wall material solution at a weight ratio of 15:1 to obtain a second emulsion; ⑥ After the second emulsion was homogenized three times under 10 MPa, it was spray-dried under the conditions of inlet air temperature of 170°C, outlet air temperature of 70°C, feed temperature of 2°C and atomization pressure of 0.8 MPa to obtain composite DHA microcapsules.

[0028] After testing, the embedding rate of the obtained composite DHA microcapsules was 96.32%, the particle size was 50-100µm, and the peroxide value after storage for 30 days was 2.45mmol / kg.

[0029] Preparation Example 2 of Composite DHA Microcapsules ① Mix 25kg of algae oil, 12kg of fish oil, 2kg of krill oil, 0.04kg of antioxidant and 0.06kg of emulsifier to obtain a core material; wherein the antioxidant includes tea polyphenol palmitate, curcumin, L-ascorbyl palmitate, and vitamin E in a weight ratio of 4:3:2:2; the emulsifier includes lactic acid fatty acid glyceride and gum arabic in a weight ratio of 3:1.

[0030] ② Dissolve chitosan, β-cyclodextrin and sodium alginate in water at a weight ratio of 4:3:1 to obtain a first wall material solution with a mass fraction of 12%; ③ Dissolve corncob powder, gelatin, xanthan gum and glycerin in a weight ratio of 11:6:4:1 in water, heat to 85°C, and obtain a second wall material solution with a mass fraction of 8wt%; ④ Mixing the first wall material solution and the core material at a weight ratio of 20:1, stirring and emulsifying at a high speed of 9000 rpm for 15 minutes to obtain a first emulsion; ⑤ Mixing the second emulsion and the second wall material solution at a weight ratio of 15:1 to obtain a second emulsion; ⑥ After homogenizing the second emulsion twice under 15 MPa, spray drying was performed under the conditions of inlet air temperature of 175°C, outlet air temperature of 75°C, feed temperature of 30°C and atomization pressure of 0.7 MPa to obtain composite DHA microcapsules.

[0031] After testing, the embedding rate of the obtained composite DHA microcapsules was 98.11%, the particle size was 50-100µm, and the peroxide value after storage for 30 days was 2.24mmol / kg.

[0032] Preparation Example 3 of Composite DHA Microcapsules ① Mix 30kg of algae oil, 15kg of fish oil, 3kg of krill oil, 0.06kg of antioxidant and 0.08kg of emulsifier to obtain a core material; wherein the antioxidant includes tea polyphenol palmitate, curcumin, L-ascorbyl palmitate, and vitamin E in a weight ratio of 5:4:3:3; the emulsifier includes lactic acid fatty acid glyceride and gum arabic in a weight ratio of 4:1.

[0033] ② Dissolve chitosan, β-cyclodextrin and sodium alginate in water at a weight ratio of 5:4:1 to obtain a first wall material solution with a mass fraction of 15%; ③ Dissolve corncob powder, gelatin, xanthan gum and glycerin in a weight ratio of 12:7:5:1 in water, heat to 90°C, and obtain a second wall material solution with a mass fraction of 10wt%; ④ Mixing the first wall material solution and the core material at a weight ratio of 20:1, stirring and emulsifying at a high speed of 10000 rpm for 10 minutes to obtain a first emulsion; ⑤ Mixing the second emulsion and the second wall material solution at a weight ratio of 15:1 to obtain a second emulsion; ⑥ After homogenizing the second emulsion once under 20 MPa, spray drying was performed under the conditions of inlet air temperature of 180°C, outlet air temperature of 80°C, feed temperature of 40°C and atomization pressure of 0.6 MPa to obtain composite DHA microcapsules.

[0034] After testing, the embedding rate of the obtained composite DHA microcapsules was 97.79%, the particle size was 50-100µm, and the peroxide value after storage for 30 days was 2.20mmol / kg.

[0035] Preparation Example 4 of Composite DHA Microcapsules ① Mix 20kg of algae oil, 10kg of fish oil, 1kg of krill oil, 0.02kg of antioxidant and 0.05kg of emulsifier to obtain a core material; wherein the antioxidant includes tea polyphenol palmitate, curcumin, L-ascorbyl palmitate, and vitamin E in a weight ratio of 3:2:1:1; the emulsifier includes lactic acid fatty acid glyceride and gum arabic in a weight ratio of 2:1.

[0036] ② Dissolve chitosan, β-cyclodextrin and sodium alginate in water at a weight ratio of 3:2:1 to obtain a first wall material solution with a mass fraction of 10-15%; ③ Dissolve corncob powder, gelatin, xanthan gum and glycerin in a weight ratio of 10:5:3:1 in water, heat to 80°C, and obtain a second wall material solution with a mass fraction of 5wt%; ④ Mixing the first wall material solution and the core material at a weight ratio of 20:1, stirring and emulsifying at a high speed of 8000 rpm for 20 minutes to obtain a first emulsion; ⑤ After homogenizing the first emulsion three times under 10 MPa, spray-drying was performed under the conditions of inlet air temperature of 170°C, outlet air temperature of 70°C, feed temperature of 2°C and atomization pressure of 0.8 MPa to obtain composite DHA microcapsules.

[0037] After testing, the embedding rate of the obtained composite DHA microcapsules was 92.39%, the particle size was 50-100µm, and the peroxide value after storage for 30 days was 3.77mmol / kg.

[0038] It can be seen that when preparing composite DHA microcapsules, two wall material embedding treatments can effectively prolong the rancidity of oils and fats, thereby retaining the physiological activity of DHA and improving the utilization rate of DHA by laying hens.

[0039] Preparation Example 1 of Premix 40 kg of puffed corn flour, 30 kg of composite DHA microcapsules, 2 kg of krill meal, 2 kg of freeze-dried sea cucumber powder, 2 kg of cottonseed oligosaccharides, 0.5 kg of DL-methionine, 0.2 kg of trace element premix, 0.02 kg of vitamin A acetate and 0.2 kg of vitamin D3 are mixed evenly to obtain a premix; wherein, the composite DHA microcapsules are prepared by Preparation Example 1 of the composite DHA microcapsules.

[0040] Preparation Example 2 of Premix 45 kg of puffed corn flour, 35 kg of composite DHA microcapsules, 3 kg of krill meal, 3 kg of freeze-dried sea cucumber powder, 3.5 kg of cottonseed oligosaccharides, 1.2 kg of DL-methionine, 1 kg of trace element premix, 0.03 kg of vitamin A acetate and 30.3 kg of vitamin D are mixed evenly to obtain a premix; wherein, the composite DHA microcapsules are prepared by Preparation Example 2 of the composite DHA microcapsules.

[0041] Preparation Example 3 of Premix 50 kg of puffed corn flour, 40 kg of composite DHA microcapsules, 4 kg of krill meal, 4 kg of freeze-dried sea cucumber powder, 5 kg of cottonseed oligosaccharides, 2 kg of DL-methionine, 2 kg of trace element premix, 0.04 kg of vitamin A acetate and 0.4 kg of vitamin D3 were mixed evenly to obtain a premix; wherein the composite DHA microcapsules were prepared by Preparation Example 3 of the composite DHA microcapsules.

[0042] Preparation Example 4 of Premix: The main difference between this Preparation Example and Preparation Example 1 of Prepreg is that the composite DHA microcapsules are prepared by Preparation Example 4 of Composite DHA Microcapsules.

[0043] Example Example 1: A method for producing eggs rich in DHA nutrients 200 kg of corn flour, 80 kg of fermented soybean meal, 20 kg of shell powder, 10 kg of sea buckthorn seed meal, 3 kg of premix and 4 kg of corn oil are uniformly mixed to obtain feed; wherein the premix is ​​prepared according to Preparation Example 1 of the premix.

[0044] During the egg-laying period, the laying hens are fed the above-mentioned laying hen feed for 20 consecutive days to obtain eggs rich in DHA nutrients.

[0045] Example 2: A method for producing eggs rich in DHA nutrients 220 kg of corn flour, 90 kg of fermented soybean meal, 25 kg of shell powder, 15 kg of sea buckthorn seed meal, 4 kg of premix and 5 kg of corn oil are uniformly mixed to obtain feed; wherein the premix is ​​prepared according to Preparation Example 2 of the premix.

[0046] During the egg-laying period, the laying hens are fed the above-mentioned laying hen feed for 20 consecutive days to obtain eggs rich in DHA nutrients.

[0047] Example 3: A method for producing eggs rich in DHA nutrients 220 kg of corn flour, 100 kg of fermented soybean meal, 30 kg of shell powder, 20 kg of sea buckthorn seed meal, 5 kg of premix and 6 kg of corn oil were uniformly mixed to obtain feed; wherein the premix was prepared according to Preparation Example 3 of the premix.

[0048] During the egg-laying period, the laying hens are fed the above-mentioned laying hen feed for 20 consecutive days to obtain eggs rich in DHA nutrients.

[0049] Example 4: The main difference between this example and Example 1 is that the premix is ​​prepared by Preparation Example 4 of the premix.

[0050] Comparative Example 1: A method for producing laying hen feed, comprising the following steps: 55 kg of corn flour, 35 kg of soybean meal, 4 kg of wheat bran, 1.75 kg of calcium hydrogen phosphate, 1.2 kg of corn oil, 1 kg of stone powder, 0.5 kg of trace element premix, 0.1 kg of choline chloride, 0.08 kg of lysine, and 0.5 kg of methionine were uniformly mixed to obtain laying hen feed.

[0051] During the egg-laying period, the laying hens are fed the above laying hen feed for 20 consecutive days to obtain eggs.

[0052] Performance Testing 150 healthy laying hens of 30-38 weeks of age, similar weight and egg production rate were selected and randomly divided into 5 groups, each with 30 hens, and the laying hens were fed by the methods of Examples 1-4 and Comparative Example 1. The test period was 20 days, and the daily feed intake and egg production rate of the laying hens were counted, and the average daily feed intake and average daily egg production rate of the laying hens on the 5th, 10th, 15th and 20th days of feeding were calculated respectively.

[0053] Three eggs were randomly selected from each group, and the DHA content in the egg yolk was tested. The test results are recorded in Table 1.

[0054] Table 1

[0055] From the data in Examples 1-4, Comparative Example 1 and Table 1, it can be seen that feeding the feed of the present application to laying hens during the egg-laying period can effectively achieve the enrichment of egg yolk DHA. On the 5th day of feeding, the egg yolk DHA content can reach more than 100 mg / 100 g. On the 15th-20th day of feeding, the egg yolk DHA content can reach more than 300 mg / 100 g. Therefore, in order to obtain a relatively stable content of egg yolk DHA, the feed of the present application can be used to feed laying hens continuously for more than 15 days.

[0056] Combining Example 1, Example 4 and the data in Table 1, it can be seen that on the 5th to 10th day of feeding laying hens, the yolk DHA content of Example 1 is significantly higher than that of Example 4, indicating that laying hens have different utilization rates for composite DHA microcapsules prepared by different methods. The composite DHA microcapsules prepared by Preparation Example 1 of the composite DHA microcapsules can not only effectively prolong the physiological activity of oil DHA, but also prolong the residence time of the composite DHA microcapsules in the stomach and intestines of laying hens due to two wall material embedding treatments, thereby improving the bioavailability of the composite DHA microcapsules to the laying hens, so as to effectively enrich the yolk DHA. On the 15th to 20th day of feeding laying hens, the yolk DHA content of Example 1 is relatively close to that of Example 4, indicating that after a long period of continuous feeding, the enrichment of yolk DHA can also be effectively improved, thereby increasing the content of yolk DHA in eggs.

[0057] In summary, when feeding laying hens using the method of the present application, the enrichment of DHA in egg yolk can be effectively achieved. When feeding for more than 15 days continuously, the DHA content in egg yolk reaches more than 300 mg / 100 g. In addition, the sources of DHA in the present application are algae oil, fish oil, krill oil, krill powder, sea cucumber freeze-dried powder, etc., and the composite DHA obtained can provide laying hens with richer nutrients to promote the growth of laying hens and increase the egg production rate of laying hens.

[0058] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A method for producing eggs rich in DHA nutrients, characterized in that: include: During the egg-laying period, feed the laying hens with laying feed for more than 15 days to obtain eggs rich in DHA nutrients; The feed comprises the following raw materials in parts by weight: 200-220 parts of corn flour, 80-100 parts of fermented soybean meal, 20-30 parts of shell powder, 10-20 parts of sea buckthorn seed meal, 3-5 parts of premix and 4-6 parts of corn oil; The premix comprises the following raw materials in parts by weight: 40-50 parts of puffed corn flour, 30-40 parts of composite DHA microcapsules, 2-4 parts of krill powder, 2-4 parts of freeze-dried sea cucumber powder, 2-5 parts of cottonseed oligosaccharides, 0.5-2 parts of DL-methionine, 0.2-2 parts of trace element premix, 0.02-0.04 parts of vitamin A acetate and 0.2-0.4 parts of vitamin D3.

2. The method for producing eggs rich in DHA nutrients according to claim 1, characterized in that: The composite DHA microcapsule comprises a core material, a first wall material embedding the core material, and a second wall material embedding the first wall material; The core material comprises the following raw materials in parts by weight: 20-30 parts of algae oil, 10-15 parts of fish oil, 1-3 parts of krill oil, 0.02-0.06 parts of antioxidant, 0.05-0.08 parts of emulsifier; The first wall material comprises chitosan, β-cyclodextrin and sodium alginate in a weight ratio of 3-5:2-4:1; The second wall material comprises corn cob powder, gelatin, xanthan gum and glycerin in a weight ratio of 10-12:5-7:3-5:

1.

3. The method for producing eggs rich in DHA nutrients according to claim 1, characterized in that: The antioxidant comprises tea polyphenol palmitate, curcumin, L-ascorbyl palmitate and vitamin E in a weight ratio of 3-5:2-4:1-3:1-3.

4. The method for producing eggs rich in DHA nutrients according to claim 2, characterized in that: The emulsifier comprises lactic acid fatty acid glyceride and gum arabic in a weight ratio of 2-4:

1.

5. The method for producing eggs rich in DHA nutrients according to any one of claims 2 to 4, characterized in that: The composite DHA microcapsules are prepared by the following method: Mixing the algae oil, the fish oil, the krill oil, the antioxidant and the emulsifier to obtain the core material; Dissolving the chitosan, the β-cyclodextrin and the sodium alginate in water to obtain a first wall material solution with a mass fraction of 10-15%; Dissolving the corncob powder, the gelatin, the xanthan gum, and the glycerin in water, and heating to 80-90° C. to obtain a second wall material solution with a mass fraction of 5-10 wt %; The first wall material solution is mixed with the core material, and emulsified by high-speed stirring to obtain a first emulsion; mixing the second emulsion with the second wall material solution to obtain a second emulsion; The second emulsion is homogenized and spray-dried to obtain the composite DHA microcapsules.

6. The method for producing eggs rich in DHA nutrients according to claim 5, characterized in that: The high-speed stirring emulsification includes: stirring for 10-20 minutes at a rotation speed of 8000-10000 rpm.

7. The method for producing eggs rich in DHA nutrients according to claim 5, characterized in that: The homogenization pressure is 10-20 MPa, and the homogenization times are 1-3 times.

8. The method for producing eggs rich in DHA nutrients according to claim 5, characterized in that: The inlet air temperature of the spray drying is 170-180°C, the outlet air temperature is 70-80°C, the feed temperature is 25-40°C, and the atomization pressure is 0.6-0.8MPa.

9. An egg rich in DHA nutrients, characterized in that: The method is produced according to any one of claims 1 to 8.

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