Multifunctional cellulolytic enzyme and application thereof in degradation of agricultural by-products

By developing the multifunctional cellulase RuCelI, the problem of insufficient adaptability of enzyme preparations in the rumen environment of ruminants has been solved, realizing the efficient degradation and utilization of crop by-products and improving economic benefits.

CN121006347AActive Publication Date: 2025-11-25JIANGXI AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202511535853.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-10-27
Publication Date
2025-11-25
Estimated Expiration
2045-10-27

AI Technical Summary

Technical Problem

Existing enzyme preparations do not adapt well to the temperature and pH ranges in the rumen environment of ruminants, resulting in low degradation efficiency of cellulose and hemicellulose, which limits the utilization rate of crop by-products and the improvement of animal production performance.

Method used

A multifunctional cellulase RuCelI was developed, which has dual activities of endonuclease and xylanase, tolerates a wide pH range, is heat resistant, and is suitable for degrading agricultural by-products.

Benefits of technology

It improves the degradation efficiency and utilization rate of crop by-products, enhances economic benefits, and provides new enzyme sources and technical support for the efficient resource utilization of crop by-products.

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Abstract

The invention discloses a multifunctional cellulolytic enzyme and application thereof in degradation of agricultural by-products, and belongs to the technical field of biology. The multifunctional cellulolytic enzyme is named RuCelI, and the amino acid sequence of the multifunctional cellulolytic enzyme is as shown in SEQ ID NO. 1. Based on a rumen microbial metagenome resource mining technology, a novel multifunctional cellulose decomposition enzyme RuCelI is developed, and the enzyme has double activities of endo-cellulase and xylanase, can be used for degrading agricultural by-products, reducing the treatment cost and improving the degradation efficiency, and is beneficial to improving the utilization rate and the economic benefit of the agricultural by-products.
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