Monoclonal antibodies reactive with glycopeptides and uses thereof

By preparing monoclonal antibodies with the fucose and peptide moieties of glycopeptides as epitopes, the problem of unclear antibody epitopes in existing technologies has been solved, enabling specific recognition and detection of fucosylated AFP, which is suitable for immunological assays such as ELISA.

CN107857815BActive Publication Date: 2026-03-17SYSMEX CORP
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Patent Information

Application Number
CN201710202207.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Priority Date
2016-10-17
Filing Date
2017-03-30
Publication Date
2026-03-17
Estimated Expiration
2037-03-30

AI Technical Summary

Technical Problem

In existing technologies, the epitopes of antibodies that bind to LCA-bound AFP are not clearly defined, which may lead to non-specific binding and make it difficult to effectively detect fucosylated AFP.

Method used

A monoclonal antibody was developed that uses the amino acid sequences of the fucosylation and peptide moieties of a glycopeptide as epitopes. By immunizing animals and screening hybridomas, an antibody capable of specifically recognizing fucosylated AFP was prepared.

Benefits of technology

It enables the specific recognition and detection of fucosylated AFP, improving the accuracy and precision of detection, and is applicable to immunological assays such as ELISA and protein blotting.

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Abstract

The present invention relates to a monoclonal antibody reacting with a glycopeptide and use thereof. The present invention provides a monoclonal antibody which is an epitope of both a fucose moiety and an amino acid of a peptide moiety. The antibody of the present invention is a monoclonal antibody reacting with a glycopeptide containing a core fucose and an amino acid of 4 or more residues continuously from an asparagine of an additional sugar chain toward a C-terminal side, the antibody being an epitope of both the core fucose of the glycopeptide and an amino acid of the glycopeptide which is 3 or more residues from the asparagine of the additional sugar chain toward the C-terminal side.
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