A device for storing liquid microbial inoculants

By designing storage devices for liquid microbial agents, including isolation layers and anti-blocking and breathable devices, the problems of bloating and contamination in the storage of liquid microbial agents are solved, extending the shelf life and reducing costs.

CN110804535BActive Publication Date: 2025-04-11SICHUAN XINLANYUE BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN201911279879.7
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2019-12-13
Publication Date
2025-04-11
Estimated Expiration
2039-12-13

AI Technical Summary

Technical Problem

In the prior art, the storage of liquid microbial agents has problems of packaging bloating and contamination, especially fungal pollution has a serious impact on quality, and the existing anti-pollution method is not suitable for non-bacillus liquid microbial agents, and the preservatives will inhibit the number of live bacteria.

Method used

A device for storing liquid microbial agents is designed, including a storage housing and an anti-blocking breathable device. An isolation layer is provided in the storage housing, and the anti-blocking and breathable device is composed of a breathable membrane, a buffer cavity and a reverse funnel. The breathable membrane is a hydrophobic and oleophobic micro-porous and leak-proof breathable membrane. The buffer cavity and the reverse funnel are connected through a buffer port, and a boss and a buffer bead are provided on the inner wall of the buffer port.

Benefits of technology

The isolation layer avoids surface bacteria contamination, and the anti-blocking and breathable device reduces the coverage of liquid microbial bacteria agents and the isolation layer on the breathable membrane, avoids bloating, prolongs the shelf life, and reduces storage costs.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention relates to the technical field of microbial liquid microbial inoculant storage devices, and in particular to a device for storing liquid microbial inoculants, comprising: an isolation layer is provided in a storage shell; an opening is provided at the top of the storage shell; an anti-blocking breathable device comprises a breathable membrane, a buffer cavity and a reverse funnel from top to bottom, the bottom of the reverse funnel is connected to the opening of the storage shell, the buffer cavity is connected to the reverse funnel through a buffer port, and a boss is provided on the inner wall of the buffer port. The present invention solves the problem of easy packaging bloating and liquid microbial inoculant contamination in the prior art, and has the advantages of reasonable and practical structural design, simple and efficient, low cost, extended shelf life of liquid microbial inoculants, and easy promotion and use.
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Description

Technical Field

[0001] The present invention relates to the technical field of liquid microbial microbial inoculant storage devices, and in particular to a device for storing liquid microbial inoculants. Background Art

[0002] In the prior art, the storage method of liquid microbial agents has the following problems:

[0003] 1. The packaging barrels of liquid microbial inoculants are generally not sterilized, because most of them cannot withstand high temperatures and cannot be sterilized at high temperatures. If the packaging barrels are sterilized by chemical or irradiation methods, the sterilization cost will be too high.

[0004] 2. Generally, liquid microbial agents are contained in wide-mouthed containers such as stacking barrels. The bacteria are easy to grow on the surface of the liquid to form a biofilm, which affects the appearance. The surface is also easily contaminated by miscellaneous bacteria, especially fungal contamination, which will seriously affect the quality.

[0005] 3. Preventing bacterial contamination by adding sodium benzoate, glycine, potassium sorbate, etc. has certain limitations. This method can only be used for Bacillus liquid microbial agents. Appropriate amounts of preservatives such as sodium benzoate, glycine, potassium sorbate, etc. will not kill spores. However, this method cannot prevent bacterial contamination of non-Bacillus liquid microbial agents. Adding sodium benzoate, glycine, potassium sorbate, etc. will inhibit the liquid microbial agent itself, resulting in a decrease in the number of effective live bacteria.

[0006] 4. The method of introducing nitrogen to seal can reduce the respiration of liquid microbial agents. This method is only suitable for the preservation of strictly aerobic liquid microbial agents and cannot solve the gas production problem of facultative anaerobic liquid microbial agents.

[0007] 5. Under the current packaging conditions, the liquid microbial agent itself or the isolation layer on the surface will form a "water film or oil film" on the surface of the breathable membrane. In addition, the bacterial film formed by the contamination of miscellaneous bacteria will also cover the breathable film, resulting in poor ventilation. Summary of the invention

[0008] In view of the above-mentioned shortcomings of the prior art, the purpose of the present invention is to provide a device for storing liquid microbial agents, which is used to solve the problems of packaging bloating and liquid microbial agent contamination in the prior art. The structural design of the present invention is reasonable and practical, simple and efficient, low-cost, and does not require anti-pollution formula treatment for the liquid microbial agent itself, which can extend the shelf life of the liquid microbial agent and facilitate its promotion and use.

[0009] To achieve the above-mentioned and other related purposes, the present invention provides a device for storing liquid microbial inoculants, the device for storing liquid microbial inoculants comprising:

[0010] A storage shell, wherein an isolation layer is disposed in the storage shell; and an opening is disposed on the top of the storage shell;

[0011] The anti-blocking and breathable device comprises a breathable membrane, a buffer cavity and a reverse funnel from top to bottom, the bottom of the reverse funnel is connected to the opening of the storage shell, the buffer cavity is connected to the reverse funnel through a buffer port, and a boss is provided on the inner wall of the buffer port.

[0012] By designing the isolation layer to float above the liquid microbial agent, the substances in the isolation layer do not inhibit the liquid microbial agent itself, thus avoiding contamination by surface bacteria, especially fungi. There is no need to sterilize the packaging barrel, package under strict aseptic conditions, or prepare the liquid microbial agent itself to prevent contamination and inhibit activity. The anti-blocking breathable device effectively prevents the vibration during transportation from covering and blocking the breathable membrane with the liquid microbial agent or the isolation layer, thereby causing flatulence. After the liquid enters the buffer chamber, it can quickly flow back downward, reducing the probability of the liquid microbial agent itself and the surface isolation layer covering the breathable membrane, which is conducive to the rapid discharge of gas.

[0013] Furthermore: the lower part of the storage shell is cylindrical, and the upper part of the storage shell is conical.

[0014] The tapered structure with a small top and a large bottom physically reduces the contact area between the liquid microbial agent and the air, and reduces the amount of the isolation layer on the surface of the liquid microbial agent in the container and the probability of contamination.

[0015] Furthermore, the lower part of the storage shell is cylindrical, and the upper part of the storage shell is provided with an inclined side plate. The tapered structure with a small top and a large bottom physically reduces the contact area between the liquid microbial agent and the air, and reduces the amount of the isolation layer on the surface of the liquid microbial agent in the container and the probability of contamination.

[0016] Further: a handle is provided on the upper part of the storage housing. The handle is provided to facilitate lifting

[0017] Furthermore: a liquid microbial inoculant is installed in the cavity of the storage shell, and the isolation layer covers the liquid microbial inoculant.

[0018] The liquid microbial inoculant is stored in the container body and sealed with an isolation layer.

[0019] Furthermore: the isolation layer is a mixture of a water-insoluble preservative and a non-volatile oil after homogenization and emulsification; the water-insoluble preservative is one or at least one of sorbic acid, benzoic acid, and methyl paraben; the non-volatile oil is one or at least one of a vegetable oil and paraffin oil having a density less than water.

[0020] The isolation layer is a homogeneous emulsion formed by one or a combination of water-insoluble preservatives, vegetable oil, and paraffin oil. It can isolate liquid microbial agents from the air, protect the microbial agents from being killed by preservatives and maintain a low metabolic rate, which is beneficial to extending the shelf life of the microbial agents. In addition, the isolation layer can greatly reduce the amount of preservatives used.

[0021] Furthermore: the mass ratio of the water-insoluble preservative to the non-volatile oil in the isolation layer is (0.06-0.5):100; the thickness of the isolation layer is ≤1 mm.

[0022] Furthermore: buffer beads are installed on the boss.

[0023] The inner wall of the buffer port is provided with a boss, on which freely movable buffer beads are placed. After the liquid enters the buffer cavity, it quickly flows back downward through the groove channel between the two bosses, reducing the probability of the bacterial agent itself and the surface isolation layer covering the breathable membrane, which is conducive to the rapid discharge of gas.

[0024] Further, the breathable membrane is a hydrophobic and oleophobic microporous leakproof breathable membrane, which can prevent a small amount of liquid and oil that enters the buffer cavity under severe shock conditions from covering the breathable membrane and affecting the breathability.

[0025] As described above, a device for storing liquid microbial agents of the present invention has the following beneficial effects: by designing the isolation layer to float above the liquid microbial agent, the substances in the isolation layer do not inhibit the liquid microbial agent itself, thus avoiding contamination by surface bacteria, especially fungi. There is no need to sterilize the packaging barrel, there is no need to package under strict aseptic conditions, and there is no need to prepare the liquid microbial agent itself for pollution prevention and activity inhibition. The anti-blocking breathable device effectively prevents vibrations during transportation from covering and blocking the breathable membrane with the liquid microbial agent or the isolation layer, thereby causing flatulence. After the liquid enters the buffer chamber, it can quickly flow back downward, reducing the probability of the liquid microbial agent itself and the surface isolation layer covering the breathable membrane, which is conducive to the rapid discharge of gas. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 Shown is a front view of a device for storing liquid microbial agents in Example 1 of the present invention.

[0027] Figure 2 The diagram shows a tilted state of a device for storing liquid microbial agents in Example 1 of the present invention.

[0028] Figure 3 Shown is a front view of a device for storing liquid microbial agents in Example 2 of the present invention.

[0029] Figure 4The diagram shows a tilted state of a device for storing liquid microbial agents in Example 2 of the present invention.

[0030] Figure 5 Shown is a side view of a storage shell for storing liquid microbial inoculants in Example 2 of the present invention.

[0031] Figure 6 Shown is a schematic diagram of an anti-blocking and breathable device for storing liquid microbial agents in an embodiment of the present invention.

[0032] Figure 7 Shown is a schematic diagram of a buffer port for storing liquid microbial inoculants in an embodiment of the present invention.

[0033] Component number description

[0034] 1-storage shell; 2-handle; 3-liquid microbial agent; 4-isolation layer; 5-breathable membrane; 6-buffer cavity; 7-reverse funnel; 8-buffer port; 9-boss; 10-buffer bead. DETAILED DESCRIPTION

[0035] The following is a description of the implementation of the present invention by means of specific embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0036] See also Figures 1 to 7 . It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings in this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions for the implementation of the present invention, so they have no substantial technical significance. Any modification of the structure, change in the proportional relationship, or adjustment of the size should still fall within the scope of the technical content disclosed by the present invention without affecting the effects and purposes that can be achieved by the present invention. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of the present invention. Changes or adjustments in their relative relationships should also be regarded as the scope of the implementation of the present invention without substantially changing the technical content.

[0037] Example 1

[0038] See also Figure 1 The present invention provides a device for storing liquid microbial inoculants, the device for storing liquid microbial inoculants comprising:

[0039] See also Figure 1, a storage shell 1, the top of which is provided with an opening; the lower part of the storage shell 1 is cylindrical, and the upper part of the storage shell 1 is conical; the upper part of the storage shell 1 is provided with a handle 2; a liquid microbial inoculant 3 is installed in the cavity of the storage shell 1, and the upper part of the liquid microbial inoculant 3 is covered with the isolation layer 4;

[0040] The mass ratio of the water-insoluble preservative (sorbic acid) to the non-volatile oil (vegetable oil) in the isolation layer 4 is 0.5:100; the thickness of the isolation layer is 0.08 mm.

[0041] See also Figure 6 , anti-blocking and breathable device, the anti-blocking and breathable device includes a breathable membrane 5, a buffer cavity 6 and a reverse funnel 7 from top to bottom, the bottom of the reverse funnel 7 is connected to the opening of the storage shell 1, and the buffer cavity 6 is connected to the reverse funnel 7 through a buffer port 8, please refer to Figure 7 The inner wall of the buffer port 8 is provided with a boss 9, and a buffer bead 10 is installed on the boss 9. The breathable membrane 5 is a hydrophobic and oleophobic microporous leak-proof breathable membrane.

[0042] Working process: the liquid microbial agent 3 is transferred into the storage shell 1, and then the isolation layer 4 is added, the isolation layer 4 covers the surface of the liquid microbial agent 3, and the reverse funnel 7 is connected with the buckle.

[0043] Example 2

[0044] See also Figure 3 The present invention provides a device for storing liquid microbial inoculants, the device for storing liquid microbial inoculants comprising:

[0045] See also Figure 3 , a storage housing 1, wherein the top of the storage housing 1 is provided with an opening; see Figure 5 The lower part of the storage shell 1 is cylindrical, and the upper part of the storage shell 1 is provided with an inclined side plate; the upper part of the storage shell 1 is provided with a handle 2; the cavity of the storage shell 1 is installed with a liquid microbial inoculant 3, and the upper part of the liquid microbial inoculant 3 is covered with the isolation layer 4;

[0046] The mass ratio of the water-insoluble preservative (methyl paraben) to the non-volatile oil (paraffin oil) in the isolation layer 4 is 0.06:100; the thickness of the isolation layer is 0.05 mm.

[0047] See also Figure 6, anti-blocking and breathable device, the anti-blocking and breathable device includes a breathable membrane 5, a buffer cavity 6 and a reverse funnel 7 from top to bottom, the bottom of the reverse funnel 7 is connected to the opening of the storage shell 1, and the buffer cavity 6 is connected to the reverse funnel 7 through a buffer port 8, please refer to Figure 7 The inner wall of the buffer port 8 is provided with a boss 9, and a buffer bead 10 is installed on the boss 9. The breathable membrane 5 is a hydrophobic and oleophobic microporous leak-proof breathable membrane.

[0048] The working process is the same as that of Example 1.

[0049] In summary, the structural design of the present invention is reasonable and practical, simple and efficient, low-cost, and does not require anti-pollution formulation treatment for the liquid microbial agent itself, which can extend the shelf life of the liquid microbial agent and facilitate its promotion and use. Therefore, the present invention effectively overcomes various shortcomings in the prior art and has a high industrial utilization value.

[0050] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A device for storing liquid microbial inoculants, characterized in that: The device for storing liquid microbial inoculants comprises: A storage shell (1), wherein an isolation layer (4) is provided in the storage shell (1); and an opening is provided at the top of the storage shell (1); An anti-blocking and breathable device, the anti-blocking and breathable device comprising, from top to bottom, a breathable membrane (5), a buffer cavity (6) and a reverse funnel (7), the bottom of the reverse funnel (7) being connected to the opening of the storage shell (1), the buffer cavity (6) being connected to the reverse funnel (7) via a buffer port (8), and a boss (9) being provided on the inner wall of the buffer port (8).

2. A device for storing liquid microbial agents according to claim 1, characterized in that: The lower part of the storage shell (1) is cylindrical, and the upper part of the storage shell (1) is conical.

3. The device for storing liquid microbial agents according to claim 1, characterized in that: The lower part of the storage shell (1) is cylindrical, and the upper part of the storage shell (1) is provided with an inclined side plate.

4. A device for storing liquid microbial agents according to claim 2 or 3, characterized in that: A handle (2) is provided on the upper portion of the storage housing (1).

5. The device for storing liquid microbial agents according to claim 1, characterized in that: The liquid microbial inoculant (3) is installed in the cavity of the storage shell (1), and the isolation layer (4) covers the top of the liquid microbial inoculant (3).

6. A device for storing liquid microbial agents according to claim 1 or 5, characterized in that: The isolation layer (4) is a homogeneously emulsified mixture of a water-insoluble preservative and a non-volatile oil.

7. The homogeneously emulsified mixture of a water-insoluble preservative and a non-volatile oil according to claim 6, characterized in that: The water-insoluble preservative is at least one of sorbic acid, benzoic acid, and methyl paraben, and the non-volatile oil is at least one of vegetable oil and paraffin oil having a density less than that of water.

8. The device for storing liquid microbial agents according to claim 6, characterized in that: The mass ratio of the water-insoluble preservative to the non-volatile oil in the isolation layer (4) is (0.06-0.5):100; the thickness of the isolation layer (4) is ≤1 mm.

9. The device for storing liquid microbial agents according to claim 7, characterized in that: A buffer bead (10) is mounted on the boss (9).

10. The device for storing liquid microbial agents according to claim 1, characterized in that: The breathable membrane (5) is a hydrophobic and oleophobic microporous leak-proof breathable membrane.

Citation Information

Patent Citations

  • Device for storing liquid microbial agent

    CN211339487U