An active stem cell micro 3D printing device based on PL-level piezoelectric nozzle technology
By using PL-level piezoelectric nozzle technology and screw extrusion valve system in biological 3D printing equipment, combined with visual observation devices, the problem that traditional methods cannot achieve micron-level accurate printing in single cells is solved, and high-precision spraying of single cells is achieved Ink printing.
Patent Information
- Application Number
- CN201910991116.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2019-10-18
- Publication Date
- 2024-12-24
- Estimated Expiration
- 2039-10-18
AI Technical Summary
Traditional biological 3D printing equipment cannot achieve accurate single-cell printing, inkjet printing technology has not been effectively promoted, and the molding accuracy of the extrusion method is insufficient, so it cannot achieve the micron-level accuracy of single-cells.
Design an active stem cell micro-3D printing device based on PL-grade piezoelectric spray head technology, using capillary glass tubes to communicate with cell reservoir bottles, screw extrusion valves and motor control systems, combined with visual observation devices, to achieve accurate spraying of single cells Ink printing.
The micron-level accurate printing of single cells is achieved, which exceeds the printing accuracy limitations of traditional methods and can print individual cells with high accuracy.
Smart Images
Figure CN110893679B_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of biological cell 3D printing, and in particular to an active stem cell micro 3D printing device based on PL-level piezoelectric nozzle technology. Background Art
[0002] 3D printing technology is a rapid prototyping technology that has become popular in recent years and is widely used in industrial production. Since 3D printing technology can print complex graphics based on computer three-dimensional models, it is used by the biomedical industry as a research and development device for printing samples of biological organs and printing active organs. It is suitable for assembling biological materials or living cells and constructing living tissues in vitro. Traditional biological 3D printing equipment mainly uses the extrusion method for cell printing. Although inkjet printing technology is also used, due to the technical limitations of manufacturers, inkjet printing technology is only used as a selective configuration and has not been effectively promoted. The extrusion method is currently widely used, and its molding accuracy is hundreds of microns or millimeters. The volume of a single cell is microns. The extrusion method cannot accurately print a single cell, while the printing accuracy of inkjet printing technology is tens to hundreds of microns, which can obtain effective printing of single cells. Summary of the invention
[0003] The purpose of the present invention is to solve the deficiencies of the prior art and provide an active stem cell micro 3D printing device capable of achieving single cell precise printing.
[0004] In order to achieve the above-mentioned purpose, an active stem cell micro 3D printing device based on PL-level piezoelectric nozzle technology is designed, which is characterized by comprising a plurality of piezoelectric nozzles, a plurality of cell storage bottles, the fluid channel of the piezoelectric nozzle is connected to the cell storage bottle by a capillary glass tube, a high-viscosity material extrusion device, a screw extrusion valve, an extrusion valve control motor 29 connected to a controller located in an electrical cabinet, the controller controls the rotation speed and direction of the motor, the extrusion valve control motor 29 is connected to a transmission screw 31 for synchronous rotation, so that an extrusion valve slider 30 reciprocates along the direction of the transmission screw 31, a storage barrel 32 is used to store liquid materials to be printed, an injection plug 33 is connected to the extrusion valve slider 30 for synchronous movement, and the liquid materials to be printed are extruded from an injection nozzle 34, and a visual observation device is composed of a horizontal observation device and a vertical observation device, the horizontal observation device is used for cell printing detection of the piezoelectric nozzle in the horizontal direction, and the vertical observation device is used for positioning, printing, calibration and printing effect detection of the printing material.
[0005] The gantry is installed on the mounting base plate, the vertical motion module mounting plate is installed on the gantry, the vertical motion module is installed on the vertical motion module mounting plate, the left vertical motion module slider can move up and down, and is used to move the single-cell inkjet printing system up and down, the right vertical motion module slider can move up and down, and is used to move the high-viscosity material extrusion system up and down, the longitudinal motion module is installed on the mounting base plate, the transverse motion module is installed on the longitudinal motion module slider, the printing base plate is installed on the transverse motion module slider, the transverse motion module slider can move left and right, the longitudinal motion module slider can move forward and backward, and the transverse motion module and the longitudinal motion module are used to move the printing base plate in the front, back, left and right planes.
[0006] The screw extrusion valve is installed on the right vertical motion module slider, the slider can move up and down, the curing lamp mounting block is installed on the right vertical motion module slider, the UV curing lamp is installed on the curing lamp mounting block, the strobe lamp is installed on the curing lamp mounting block, and the curing lamp mounting block moves up and down with the right vertical motion module slider.
[0007] A vertical mounting plate is provided on the left vertical motion module slider, and the slider can move up and down. The vertical camera mounting block is installed on the vertical mounting plate, the vertical zoom lens is installed on the vertical camera mounting block, and the vertical camera is installed on the vertical zoom lens. The vertical mounting plate is also provided with a liquid storage bottle mounting rack, a nozzle mounting rack and an air filter. The nozzle mounting rack is provided with four nozzle installation stations for installing piezoelectric nozzles. The liquid storage bottle mounting rack is provided with four installation stations for installing liquid storage bottles. The air pipe connects the air filter and the cell liquid storage bottle, the liquid line pipe connects the piezoelectric nozzle and the cell liquid storage bottle, the nozzle signal interface is installed on the vertical mounting plate, and the piezoelectric nozzle signal line is connected to the nozzle signal interface.
[0008] The vertical motion module mounting plate is provided with a horizontal camera mounting frame, the horizontal camera motion module is mounted on the horizontal camera mounting frame, the horizontal camera is mounted on the horizontal camera motion module, and the horizontal lens is mounted on the horizontal camera.
[0009] Compared with the prior art, the present invention has the advantages of using inkjet printing technology to replace the traditional extrusion method for cell printing, which can achieve single cell printing that the extrusion method cannot do, and at the same time, the cell precision is high and can be achieved at the micron level. BRIEF DESCRIPTION OF THE DRAWINGS
[0010] Figure 1 The structure of the present invention is schematically shown Figure 1 ;
[0011] Figure 2 The structure of the present invention is schematically shown Figure 2 ;
[0012] In the figure: 1. Left Z motion module 2. Right Z motion module 3. Screw extrusion valve 4. Z motion module mounting plate 5. Gantry 6. Mounting base plate 7. Vertical camera 8. Vertical zoom lens 9. Horizontal camera mounting frame 10. Horizontal camera motion module 11. Horizontal camera 12. Horizontal lens 13. Piezoelectric nozzle 14. Nozzle mounting frame 15. Printing base plate 16. X motion module 17. Y motion module 18. Cell storage bottle 19. UV curing lamp 20. Curing lamp mounting block 21. Strobe lamp 22. Air filter 23. Air pipe 24. Storage bottle mounting frame 25. Nozzle signal interface 26. Liquid line pipe 27. Vertical camera mounting block 28. Z mounting plate. DETAILED DESCRIPTION
[0013] The present invention will be further described below in conjunction with the accompanying drawings, and the structure and principle of this device are very clear to people in the field. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.
[0014] like Figure 1 , 2 The active stem cell micro 3D printing system based on PL-level piezoelectric nozzle technology shown includes a motion control system consisting of a left Z motion module 1, a right Z motion module 2, a Z motion module mounting plate 4, a gantry 5, a mounting base plate 6, a printing base plate 15, an X motion module 16, and a Y motion module 17; a visual observation system consisting of a vertical camera 7, a vertical zoom lens 8, a horizontal camera mounting frame 9, a horizontal camera motion module 10, a horizontal camera 11, and a horizontal lens 12; a single-cell inkjet printing system consisting of an inkjet nozzle 13, a nozzle mounting frame 14, a cell storage bottle 18, a strobe light 21, an air filter 22, an air pipe 23, a storage bottle mounting frame 24, a nozzle signal interface 25, and a liquid line tube 26; and a high-viscosity extrusion system consisting of a screw extrusion valve 3, a UV curing lamp 19, and a curing lamp mounting block 20.
[0015] like Figure 1 As shown, the gantry 5 is mounted on the mounting base plate, the Z motion module mounting plate 4 is mounted on the gantry 5, the left Z motion module 1 and the right Z motion module 2 are mounted on the Z motion module mounting plate 4, the slider of the left Z motion module 1 can move up and down, and is used to move the single-cell inkjet printing system up and down; the slider of the right Z motion module 2 can move up and down, and is used to move the high-viscosity material extrusion system up and down; the Y motion module 17 is mounted on the mounting base plate 6, the X motion module 16 is mounted on the slider of the Y motion module 17, and the printing base plate 15 is mounted on the slider of the X motion module 16, the slider of the X motion module 16 can move left and right, and the slider of the Y motion module 17 can move forward and backward, and the X motion module 16 and the Y motion module 17 are used to move the printing base plate 15 in a plane forward and backward and left and right.
[0016] like Figure 1 As shown, the screw extrusion valve 3 is installed on the slider of the right Z motion module 2 and can move up and down; the curing lamp mounting block 20 is installed on the slider of the right Z motion module 2, the ultraviolet curing lamp 19 is installed on the curing lamp mounting block 20, and the strobe lamp 21 is installed on the curing lamp mounting block 20. The curing lamp mounting block 20 can move up and down following the slider of the right Z motion module 2.
[0017] like Figure 1 , 2 As shown, the Z mounting plate 28 is mounted on the slider of the left Z motion module 1 and can move up and down; the vertical camera mounting block 27 is mounted on the Z mounting plate 28, the vertical zoom lens 8 is mounted on the vertical camera mounting block 27, the vertical camera 7 is mounted on the vertical zoom lens 8, and the vertical zoom lens 8 includes a coaxial light source; the liquid storage bottle mounting frame 24 is mounted on the Z mounting plate 28, the nozzle mounting frame 14 is mounted on the Z mounting plate 28, the inkjet nozzle 13 is mounted on the nozzle mounting frame 14, and the nozzle mounting frame 14 has four nozzle installation positions for installing nozzles; the cell storage bottle 18 is installed on the storage bottle mounting rack 24, the storage bottle mounting rack 24 has 4 installation positions for installing the storage bottle 18, the air filter 22 is installed on the Z mounting plate 28, the air pipe 23 connects the air filter 22 and the cell storage bottle 18, the liquid line pipe 26 connects the inkjet nozzle 13 and the cell storage bottle 18, the nozzle signal interface 25 is installed on the Z mounting plate 28, and the signal line of the inkjet nozzle 13 is connected to the nozzle signal interface 25.
[0018] like Figure 1 , 2 As shown, the horizontal camera mounting frame 9 is mounted on the Z motion module mounting plate 4, the horizontal camera motion module 10 is mounted on the horizontal camera mounting frame 9, the horizontal camera 11 is mounted on the horizontal camera motion module 10, and the horizontal lens 12 is mounted on the horizontal camera 11.
[0019] The specific operation method is as follows:
[0020] Add cell solution to the cell storage bottle 18, adjust the parameters of the inkjet nozzle 13 by computer, the inkjet nozzle 13 will form stable droplets, and the state of the droplets can be observed through the horizontal camera 11 and the horizontal lens 12. The advantage of the present invention is that the inkjet nozzle 11 adopts a single-channel nozzle, and the pipelines involved are Teflon and capillary glass tubes. Four types of cells can be printed in a single program at the same time. The horizontal camera 11 and the horizontal lens 12 can move back and forth under the action of the horizontal camera mounting frame, and the printing status of the four cells can be observed; add materials to the screw extrusion valve 3 and adjust its parameters by computer, and edit the motion program through the host computer software.
[0021] The extrusion valve control motor is connected to the controller located in the electrical cabinet. The controller can accurately control the rotation speed and direction of the motor. The extrusion valve control motor is connected to the transmission screw for synchronous rotation, causing the extrusion valve slider to reciprocate along the direction of the transmission screw. The storage barrel is used to store the liquid material to be printed. The injection plug is connected to the extrusion valve slider for synchronous movement to extrude the liquid material to be printed from the injection nozzle.
[0022] The screw extrusion valve is used to print high-viscosity liquid materials. With the help of the motion control system and precise computer control, 3D graphics printing can be achieved. The printed 3D model serves as a carrier for cell survival, so that subsequent inkjet cells can be accurately printed.
Claims
1. An active stem cell micro 3D printing device based on PL-level piezoelectric nozzle technology, characterized in that Including several piezoelectric nozzles, If the stem cell storage bottle, The fluid channel of the piezoelectric nozzle is connected to the cell storage bottle through a capillary glass tube. The high-viscosity material extrusion device adopts a screw extrusion valve. The extrusion valve control motor is connected to the controller located in the electrical cabinet. The controller controls the rotation speed and direction of the motor. The extrusion valve control motor is connected to the transmission screw to rotate synchronously, causing the extrusion valve slider to reciprocate along the direction of the transmission screw. The storage barrel is used to store the liquid material to be printed. The injection plug is connected to the extrusion valve slider to move synchronously to extrude the liquid material to be printed from the injection nozzle. The visual observation device is composed of a horizontal observation device and a vertical observation device. The horizontal observation device is used for the cell printing detection of the piezoelectric nozzle in the horizontal direction, and the vertical observation device is used for the positioning and printing calibration of the printing material and the printing effect detection; The gantry is installed on the mounting base plate, the vertical motion module mounting plate is installed on the gantry, the vertical motion module is installed on the vertical motion module mounting plate, the left vertical motion module slider can move up and down, and is used to move the single-cell inkjet printing system up and down, the right vertical motion module slider can move up and down, and is used to move the high-viscosity material extrusion system up and down, the longitudinal motion module is installed on the mounting base plate, the transverse motion module is installed on the longitudinal motion module slider, the printing base plate is installed on the transverse motion module slider, the transverse motion module slider can move left and right, the longitudinal motion module slider can move forward and backward, and the transverse motion module and the longitudinal motion module are used to move the printing base plate forward and backward, left and right planes; The screw extrusion valve is installed on the right vertical motion module slider, the slider can move up and down, the curing lamp mounting block is installed on the right vertical motion module slider, the UV curing lamp is installed on the curing lamp mounting block, the strobe lamp is installed on the curing lamp mounting block, and the curing lamp mounting block moves up and down with the right vertical motion module slider.
2. The active stem cell micro 3D printing device based on PL-level piezoelectric nozzle technology as claimed in claim 1, characterized in that: A vertical mounting plate is provided on the left vertical motion module slider, and the slider can move up and down. The vertical camera mounting block is installed on the vertical mounting plate, the vertical zoom lens is installed on the vertical camera mounting block, and the vertical camera is installed on the vertical zoom lens. The vertical mounting plate is also provided with a liquid storage bottle mounting rack, a nozzle mounting rack and an air filter. The nozzle mounting rack is provided with four nozzle installation stations for installing piezoelectric nozzles. The liquid storage bottle mounting rack is provided with four installation stations for installing liquid storage bottles. The air pipe connects the air filter and the cell liquid storage bottle, the liquid line pipe connects the piezoelectric nozzle and the cell liquid storage bottle, the nozzle signal interface is installed on the vertical mounting plate, and the piezoelectric nozzle signal line is connected to the nozzle signal interface.
3. The active stem cell micro 3D printing device based on PL-level piezoelectric nozzle technology as claimed in claim 1, characterized in that: The vertical motion module mounting plate is provided with a horizontal camera mounting frame, the horizontal camera motion module is mounted on the horizontal camera mounting frame, the horizontal camera is mounted on the horizontal camera motion module, and the horizontal lens is mounted on the horizontal camera.