A petri dish shooting device and a light source control method

Abstract

The embodiment of the application discloses a culture dish shooting device and a light source control method. The culture dish shooting device comprises a culture dish cover, a camera, an upper light source, a lower light source and an ultraviolet light source. The culture dish cover is disposable and barrel-shaped. The inverted cover is arranged above the culture dish to form a relatively closed environment with the culture dish, so that microorganisms in the environment cannot contaminate the culture medium in the culture dish. The upper light source is arranged above the culture dish cover or on the outer side of the culture dish cover around the camera. The lower light source is arranged below the culture dish. The ultraviolet light source is arranged on the outer side wall of the culture dish cover. The culture dish cover is arranged above the culture dish, and the camera lens is arranged in the protruding part at the bottom of the culture dish cover, so that a small culture dish shooting device is formed, and the culture dish shooting device is convenient to carry. Meanwhile, different light sources are arranged at different positions, so that different light sources can be controlled according to different scenes to obtain colony images meeting different requirements.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microorganisms, in particular to a petri dish shooting device and a light source control method. BACKGROUND

[0002] In the microbial testing of food, medicine, cosmetics and feed, the total number of colonies, coliform group, E. coli, lactic acid bacteria and other projects are involved, and a large number of colony analysis and counting are required when the statistical results are obtained. Colony refers to the visible single bacterial group on the surface of the solid culture medium after the bacteria are inoculated on the surface of the solid culture medium. Counting the number of colonies generated after sampling is a basic and important work in the relevant field. Colony image refers to the image of the colony formed by shooting the colony with a camera after the microorganism grows on the culture medium to form a colony. The colony image is of great significance to the quantitative counting, species identification, microbial growth and metabolism research of microorganisms.

[0003] Currently, the colony image is mainly obtained by large shooting equipment, which is not convenient to carry. At the same time, there is uneven illumination, which affects the accuracy of the colony counting result. The obtained microbial colony image has no correlation with the growth environment condition, and the microbial colony morphology image under the uniform illumination environment cannot be formed by precise control of the light source. SUMMARY

[0004] Due to the above problems of the existing method, the present application embodiment proposes a petri dish shooting device and a light source control method.

[0005] In a first aspect, the present application embodiment proposes a petri dish shooting device, comprising: a petri dish cover, a camera, an upper light source, a lower light source and an ultraviolet light source.

[0006] The petri dish cover is barrel-shaped, and the inverted cover is arranged above the petri dish. The center part of the bottom surface of the petri dish cover is upwardly protruding to form a protruding part.

[0007] The camera head of the camera is arranged in the protruding part, and the camera head faces the petri dish.

[0008] The upper light source is arranged above the petri dish cover and uniformly arranged around the camera head, or arranged outside the petri dish cover.

[0009] The lower light source is arranged below the petri dish.

[0010] The ultraviolet light source is arranged on the outer wall of the petri dish cover.

[0011] Optionally, the head of the camera is protrudingly arranged on the protruding part of the center of the bottom surface of the petri dish cover.

[0012] Optionally, the culture dish cover is hemispherical or barrel-shaped.

[0013] Optionally, the culture dish photographing device further comprises a communication unit and an information storage unit.

[0014] The communication unit is connected with the camera, receives the image photographed by the camera, and transmits the image and the environmental information when the image is photographed.

[0015] The information storage unit is used to store the image and the environmental information when the image is photographed.

[0016] Optionally, the camera head of the camera is arranged above the culture dish to perform normal photographing, or the camera head of the camera is arranged below the culture dish to perform inverted photographing.

[0017] Optionally, the culture dish photographing device further comprises a gyroscope.

[0018] The gyroscope is used to record the normal culture state or the inverted culture state when photographing.

[0019] The normal culture state is the state of normal photographing by the camera, and the inverted culture state is the state of inverted photographing by the camera.

[0020] Optionally, the culture medium of the culture dish is arranged to face the camera head, and the camera head obtains the image of microorganisms in the culture medium after irradiation by the upper light source.

[0021] Or,

[0022] The culture medium of the culture dish is arranged to face away from the camera head, and the camera head obtains the image of microorganisms in the culture medium after irradiation by the lower light source.

[0023] Optionally, a plurality of culture dish photographing devices are arranged in the same incubator.

[0024] Optionally, the upper light source and the lower light source form a uniform area light source through a light homogenizing plate.

[0025] Optionally, the culture dish photographing device further comprises a temperature and humidity sensor, a light intensity sensor, and a gas partial pressure sensor.

[0026] The temperature and humidity sensor, the light intensity sensor, and the gas partial pressure sensor are arranged outside the culture dish cover and inside the shell of the culture dish photographing device.

[0027] Optionally, the culture dish photographing device further comprises a temperature and humidity alarm unit.

[0028] The temperature alarm unit is connected with the temperature and humidity sensor, and an alarm is given when the temperature and humidity deviates from the preset range.

[0029] In a second aspect, the application also provides a light source method of the petri dish shooting device, comprising:

[0030] receiving an image acquisition instruction sent by the terminal;

[0031] controlling at least one of the upper light source, the lower light source and the ultraviolet light source to be turned on according to the image acquisition instruction, so as to acquire the colony image in the petri dish by the camera.

[0032] Optionally, the light source control method of the petri dish shooting device further comprises:

[0033] receiving a fluorescence acquisition instruction sent by the terminal;

[0034] controlling the upper light source and / or the ultraviolet light source to be turned on according to the fluorescence acquisition instruction, so as to acquire the fluorescence image of the colony in the petri dish by the camera.

[0035] Optionally, the controlling the upper light source and / or the ultraviolet light source to be turned on according to the fluorescence acquisition instruction, so as to acquire the fluorescence image of the colony in the petri dish by the camera specifically comprises:

[0036] controlling the upper light source and / or the ultraviolet light source to be turned on according to the fluorescence acquisition instruction, so as to acquire the fluorescence image of the colony in the petri dish by the camera through continuous shooting or switching light source shooting.

[0037] Optionally, the receiving the fluorescence acquisition instruction sent by the terminal specifically comprises:

[0038] receiving a fluorescence acquisition instruction generated by the terminal according to the inherent color of the culture medium;

[0039] The controlling the upper light source and / or the ultraviolet light source to be turned on according to the fluorescence acquisition instruction, so as to acquire the fluorescence image of the colony in the petri dish by the camera specifically comprises:

[0040] controlling the upper light source and the ultraviolet light source to synthesize light color corresponding to the inherent color of the culture medium according to the fluorescence acquisition instruction.

[0041] According to the above technical solution, the petri dish cover is arranged above the petri dish, and the camera lens of the camera is arranged in the protruding part at the bottom of the petri dish cover, so that a small petri dish shooting device is formed, which is convenient to carry. Meanwhile, different light sources are arranged at different positions, so that different light sources can be controlled to work according to different scenes, so as to obtain colony images meeting different requirements. Attached Figure Description

[0042] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0043] Figure 1 This is a schematic diagram of an upright petri dish imaging device according to an embodiment of the present invention.

[0044] Figure 2 This is a schematic diagram of an inverted petri dish imaging device according to an embodiment of the present invention.

[0045] Figure 3 This is a flowchart illustrating a light source control method for a petri dish imaging device according to an embodiment of the present invention. Detailed Implementation

[0046] The specific embodiments of the present invention will be further described below with reference to the accompanying drawings. The following examples are only used to more clearly illustrate the technical solutions of the present invention and should not be construed as limiting the scope of protection of the present invention.

[0047] Figure 1 The diagram shows a structural schematic of a petri dish imaging device provided in this embodiment, including: a petri dish cover 1, a camera 2, an upper light source 3, a lower light source 4, and an ultraviolet light source 5.

[0048] The petri dish cover 1 is barrel-shaped and is placed upside down over the petri dish 6. The center part of the bottom surface 11 of the petri dish cover 1 protrudes upward to form a protruding part 111.

[0049] The camera of the camera 2 is located inside the protrusion 111, and the camera is positioned facing the petri dish 6.

[0050] The upper light source 3 is located above the culture dish cover 1 and is evenly arranged around the camera, or it is located on the outside of the culture dish cover 1.

[0051] The lower light source 4 is located below the culture dish.

[0052] The ultraviolet light source 5 is located on the outer wall of the culture dish cover 1.

[0053] Specifically, the upper light source 3 and the lower light source 4 are both RGB synthetic light sources. Through the RGB synthetic light source, the brightness and color of the light source can be adjusted, the characteristics of the light source can be changed, the influence of the original environment background can be eliminated, and a clearer colony image can be obtained.

[0054] By using the ultraviolet light source 5, the fluorescence phenomenon of specific microorganisms can be collected, which is convenient for further fluorescence analysis of the colony image.

[0055] Specifically, through the reflection of the upper light source 3, the part of the colony in the colony image captured by the camera is bright, and the surface morphology of the colony is clear. Through the transmission of the lower light source 4, the part of the colony in the colony image captured by the camera is dark, which is opposite to the brightness of the colony image captured by the reflection of the upper light source. By processing the different brightness of the reflection image and the transmission image, a clearer colony image can be obtained.

[0056] In addition, through the irradiation of the ultraviolet light source 5, fluorescence phenomenon can be generated, and combined with the reflection of the upper light source 3 and the transmission of the lower light source 4, three different types of colony images can be obtained. When processing the colony image later, the three colony images can be superimposed to obtain more comprehensive colony information.

[0057] It should be noted that when the upper light source 3 is arranged on the bottom surface 11 inside the culture dish cover 1, there will be a certain shadow.

[0058] If the shadow has no effect on the processing of the colony image, the upper light source 3 can be arranged on the bottom surface 11 inside the culture dish cover 1, otherwise the upper light source 3 needs to be arranged on the inner side wall of the culture dish cover 1 close to the bottom surface 11, so that there will be no shadow after shooting the colony image.

[0059] The culture dish cover is disposable and barrel-shaped, and the inverted cover is arranged above the culture dish to form a relatively closed environment with the culture dish, so as to avoid contamination of the culture medium by microorganisms in the environment. The culture medium is located in the culture dish. The center of the barrel bottom is upwardly protruding; the camera lens is arranged in the protruding part, and the camera lens faces the culture dish; the upper light source is arranged above the culture dish cover or outside the culture dish cover around the camera, and the reflection image of the culture medium surface can be obtained by irradiation of the upper light source; the lower light source is arranged below the culture dish, and the transmission image of the light penetrating the culture medium can be obtained by irradiation of the lower light source; the ultraviolet light source is arranged on the outer side wall of the culture dish cover, and the ultraviolet absorption image of the culture medium can be obtained by irradiation of the ultraviolet light source.

[0060] The embodiment forms a small culture dish shooting device by covering the culture dish with a culture dish cover and setting the camera lens in the protruding part at the bottom of the culture dish cover, which is convenient to carry. Meanwhile, different light sources are set to facilitate the control of different light sources according to different scenes to obtain colony images meeting different requirements.

[0061] Further, on the basis of the above embodiment, the head of the camera is protruding at the center of the bottom of the culture dish cover.

[0062] Specifically, when the culture dish shooting device is placed as shown in Figure 1 Water in the culture dish is easy to evaporate, and water vapor is formed inside the bottom 11 of the culture dish cover 1. If the camera lens is set in the same plane as the through hole 111, water vapor is easy to form on the camera lens, which greatly affects the image shooting effect. By protruding the head of the camera lens inside the culture dish cover, the camera lens is protruding inside the culture dish cover 1, which is not conducive to the accumulation of water vapor and can shoot images with better effect.

[0063] Further, on the basis of the above embodiment, the culture dish cover 1 is hemispherical or barrel-shaped.

[0064] Specifically, the shape of the culture dish cover can be selected according to different requirements.

[0065] When it is necessary to switch the placement direction of the culture dish shooting device, a barrel-shaped culture dish cover as shown in Figure 1 and Figure 2 can be used, which can be placed on both sides.

[0066] When the culture dish shooting device is only used in the upright position, a hemispherical culture dish cover can be used, and the through hole is set on the hemispherical surface. The culture dish cover of this shape cannot be placed upside down because the spherical surface cannot be placed stably on the horizontal plane.

[0067] Generally, the cover is 10-12 cm high, which is the best for obtaining better images.

[0068] Further, on the basis of the above embodiment, the culture dish shooting device further comprises a communication unit and an information storage unit.

[0069] The communication unit is connected with the camera, receives the images shot by the camera, and transmits the images and the environmental information when the images are shot.

[0070] The information storage unit is used to store the images and the environmental information when the images are shot.

[0071] When a terminal requests a colony image, the colony image can be sent to the terminal via the communication unit. Alternatively, the colony image can be stored via the information storage unit.

[0072] Furthermore, based on the above embodiments, the camera lens is positioned above the culture dish for upright shooting, or the camera lens is positioned below the culture dish for inverted shooting.

[0073] Specifically, after fixing camera 2 inside the petri dish cover, the petri dish can be placed upright, such as... Figure 1 As shown, it can also be reversed, as shown in the image. Figure 2 As shown.

[0074] When the petri dish is inverted, such as Figure 2 As shown, the bottom of the petri dish is covered with a culture cover 8.

[0075] Furthermore, the petri dish imaging device also includes a gyroscope;

[0076] The gyroscope is used to record the upright or inverted culture state during the shooting process;

[0077] The upright culture state refers to the state in which the camera takes an upright picture, and the inverted culture state refers to the state in which the camera takes an inverted picture.

[0078] The placement status of the petri dish imaging device is recorded by a gyroscope, which facilitates the automatic acquisition of the placement status of the petri dish imaging device.

[0079] Furthermore, based on the above embodiments, the culture medium in the petri dish is positioned facing the camera, and the camera acquires images of microorganisms in the culture medium after being illuminated by the upper light source;

[0080] or,

[0081] The culture medium in the petri dish is positioned with its back to the camera, and the camera acquires images of the microorganisms in the culture medium after being illuminated by the lower light source.

[0082] Specifically, the petri dish can be placed inside the device with the lid removed and the culture cover forming a relatively closed system. At this time, the surface of the culture medium faces the camera, and the growth image of microorganisms on the surface of the culture medium can be obtained through the upper light source. Alternatively, the culture medium can be placed upside down under the culture cover. At this time, the culture medium is located inside the petri dish and faces the lower light source. The transmission image of microorganisms growing in the culture medium can be obtained through the illumination of the lower light source.

[0083] Furthermore, based on the above embodiments, several of the described petri dish imaging devices are located in the same incubator.

[0084] Specifically, the petri dish shooting device provided by the embodiment has a small volume, and multiple petri dish shooting devices can be placed in the same incubator for culture, so that the influence of environmental factors on the growth of microorganisms is eliminated.

[0085] Further, on the basis of the above embodiment, the upper light source and the lower light source form a uniform area light source through the light homogenizing plate.

[0086] Specifically, the upper light source and the lower light source form a uniform area light source through the light homogenizing plate, so as to avoid the formation of reflected light spots and affect the shooting effect.

[0087] Further, on the basis of the above embodiment, the petri dish shooting device further comprises:

[0088] The temperature and humidity sensor, the light intensity sensor, the gas partial pressure sensor and the temperature and humidity alarm unit are arranged outside the petri dish cover and in the shell of the petri dish shooting device.

[0089] The temperature and humidity sensor, the light intensity sensor and the gas partial pressure sensor are arranged outside the petri dish cover and in the shell of the petri dish shooting device.

[0090] The temperature and humidity alarm unit is connected with the temperature and humidity sensor, and an alarm is given when the temperature and humidity deviates from the preset range.

[0091] By arranging the temperature and humidity meter and the temperature and humidity alarm unit, the temperature inside the petri dish shooting device can be monitored in real time, and an alarm can be given in time when the temperature or humidity is not conducive to the growth of colonies.

[0092] Figure 3 A flowchart of a light source control method of a petri dish shooting device provided by the embodiment is shown, which comprises:

[0093] S301, receiving an image acquisition instruction sent by a terminal.

[0094] S302, controlling at least one of the upper light source, the lower light source and the ultraviolet light source to be turned on according to the image acquisition instruction, so as to acquire the colony image in the petri dish through the camera.

[0095] The image acquisition instruction is an instruction sent by the terminal to the petri dish shooting device for acquiring the colony image.

[0096] Specifically, the ultraviolet light can be used to acquire the fluorescence phenomenon of specific microorganisms, so as to facilitate further fluorescence analysis of the colony image.

[0097] The embodiment forms a small culture dish shooting device by covering the culture dish with a culture dish cover and arranging the camera of the camera in the protruding part at the bottom of the culture dish cover, and controls different light sources to work according to different scenes to obtain colony images meeting different requirements by arranging light sources at different positions.

[0098] Further, on the basis of the method embodiment, the light source control method of the culture dish shooting device further includes:

[0099] receiving the fluorescence collection instruction sent by the terminal.

[0100] controlling the upper light source and / or the ultraviolet light source to be turned on according to the fluorescence collection instruction, so as to collect the fluorescence image of the colony in the culture dish by the camera.

[0101] The fluorescence collection instruction is an instruction sent by the terminal to the culture dish shooting device for collecting the fluorescence image of the colony.

[0102] By controlling different light sources to work, colony images meeting different requirements can be obtained.

[0103] Further, on the basis of the method embodiment, the fluorescence collection instruction sent by the terminal to the culture dish shooting device for collecting the fluorescence image of the colony.

[0104] controlling the upper light source and / or the ultraviolet light source to be turned on according to the fluorescence collection instruction, so as to collect the fluorescence image of the colony in the culture dish by the camera.

[0105] Specifically, continuous shooting can be performed by a single or multiple light sources, for example, continuous shooting is performed by turning on the upper light source and the ultraviolet light source; or, the upper light source and the ultraviolet light source are switched to perform continuous shooting.

[0106] By different shooting modes, different shooting requirements can be adapted.

[0107] In another embodiment, S301 specifically includes:

[0108] receiving the fluorescence collection instruction generated by the terminal according to the inherent color of the culture medium;

[0109] Correspondingly, S302 specifically includes:

[0110] controlling the upper light source and the ultraviolet light source to synthesize the light color corresponding to the inherent color of the culture medium according to the fluorescence collection instruction.

[0111] By controlling the light color of the light source and the ultraviolet light source to correspond to the inherent color of the culture medium, a better shooting effect can be obtained.

[0112] The device embodiments described above are merely illustrative, wherein the units described as separate components can or can not be physically separated, and the components displayed as units can or can not be physical units, i.e., can be located in one place or distributed on multiple network units. Part or all of the modules can be selected to achieve the purpose of the embodiment scheme according to actual needs. Those skilled in the art can understand and implement without creative labor.

[0113] Through the description of the above embodiments, those skilled in the art can clearly understand that each embodiment can be realized by means of software and the necessary general hardware platform, and of course, it can also be realized by hardware. Based on such understanding, the above technical solutions can be embodied in the form of a software product, which can be stored in a computer readable storage medium, such as a ROM / RAM, a magnetic disk, an optical disk, etc., and includes a plurality of instructions for causing a computer device (which can be a personal computer, a server, or a network device, etc.) to execute the method described in each embodiment or some parts of the embodiment.

[0114] It should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A petri dish photographing apparatus, characterized by comprising: The application relates to a culture dish shooting device. The culture dish cover is disposable and barrel-shaped, the culture dish cover is arranged above the culture dish in an inverted manner, the center of the bottom surface of the culture dish cover is protruded upwards to form a protruded part, and the protruded part is wide at the bottom and narrow at the top. The culture dish cover and the culture dish form a relatively closed system, and the culture dish is shot. The camera head of the camera is arranged in the protruded part, and the camera head faces the culture dish. The camera head of the camera is arranged above the culture dish to shoot in an upright manner, or the camera head of the camera is arranged below the culture dish to shoot in an inverted manner. The upper light source is arranged above the culture dish cover and uniformly arranged around the camera head, or arranged outside the culture dish cover. The lower light source is arranged below the culture dish. The ultraviolet light source is arranged on the outer wall of the culture dish cover. The head of the camera head is arranged in the protruded part of the center of the bottom surface of the culture dish cover. The culture dish shooting device further comprises a temperature and humidity sensor, a light intensity sensor and a gas partial pressure sensor. The temperature and humidity sensor, the light intensity sensor and the gas partial pressure sensor are arranged outside the culture dish cover and arranged in the shell of the culture dish shooting device. The shape of the culture dish cover is replaced by a semi-spherical shape.

2. The petri dish photographing apparatus according to claim 1, wherein The culture dish shooting device further comprises a communication unit and an information storage unit.

3. The petri dish photographing apparatus according to claim 1, wherein The communication unit is connected with the camera, receives the image shot by the camera, and transmits the image and the environmental information when the image is shot. The information storage unit is used for storing the image and the environmental information when the image is shot. The culture dish shooting device further comprises a gyroscope.

4. The petri dish photographing apparatus according to claim 1, wherein The gyroscope is used for recording the upright culture state or the inverted culture state when the image is shot. The upright culture state is the state of the camera shooting in an upright manner, and the inverted culture state is the state of the camera shooting in an inverted manner. The culture medium of the culture dish faces the camera head, and the camera head obtains the image of microorganisms in the culture medium after being irradiated by the upper light source.

5. The petri dish photographing apparatus according to claim 1, wherein Or, The culture medium of the culture dish faces away from the camera head, and the camera head obtains the image of microorganisms in the culture medium after being irradiated by the lower light source. A plurality of culture dish shooting devices are arranged in the same incubator.

6. The petri dish photographing apparatus according to claim 1, wherein The upper light source and the lower light source form a uniform area light source through a uniform light plate.

7. The petri dish photographing apparatus according to claim 1, wherein The culture dish shooting device further comprises a temperature and humidity alarm unit.

8. The petri dish photographing apparatus according to claim 1, wherein The temperature and humidity alarm unit is connected with the temperature and humidity sensor, and alarms when the temperature and humidity deviates from the preset range. The application relates to a culture dish shooting device.

9. A method of controlling a light source of a petri dish shooting apparatus according to any one of claims 1 to 8, characterized by, The culture dish shooting device receives the image acquisition instruction sent by a terminal. The culture dish shooting device controls at least one of the upper light source, the lower light source and the ultraviolet light source to be turned on according to the image acquisition instruction, so as to acquire the colony image in the culture dish through the camera. The light source control method of the culture dish shooting device further comprises the following steps.

10. The light source control method of a petri dish shooting apparatus according to claim 9, wherein The culture dish shooting device receives the fluorescence acquisition instruction sent by the terminal. The culture dish shooting device controls the upper light source and / or the ultraviolet light source to be turned on according to the fluorescence acquisition instruction, so as to acquire the fluorescence image of the colony in the culture dish through the camera. ​ 11. The light source control method of the petri dish shooting apparatus according to claim 10, wherein The upper light source and / or the ultraviolet light source are controlled to be turned on according to the fluorescence acquisition instruction, and a fluorescence image of the colony in the culture dish is acquired by the camera, specifically including: The upper light source and / or the ultraviolet light source are controlled to be turned on according to the fluorescence acquisition instruction, and a fluorescence image of the colony in the culture dish is acquired by the camera, specifically including:

12. The light source control method of the petri dish shooting apparatus according to claim 10, wherein The fluorescence acquisition instruction sent by the terminal is received, specifically including: The fluorescence acquisition instruction generated by the terminal according to the inherent color of the culture medium is received; Accordingly, the upper light source and the ultraviolet light source are controlled to be turned on according to the fluorescence acquisition instruction, and a fluorescence image of the colony in the culture dish is acquired by the camera, specifically including: The upper light source and the ultraviolet light source are controlled to synthesize a light color corresponding to the inherent color of the culture medium according to the fluorescence acquisition instruction.

Images