A catheter type PCR reaction tube

Abstract

The present invention provides a catheter-type PCR reaction tube, comprising a reaction tube base; a reference plane is arranged on the reaction tube base, an injection groove is arranged on the reference plane, a plurality of reaction tube cavities are arranged on the reaction tube base around the injection groove, and the reaction tube cavities are connected with the injection groove through a guide tube; the guide tube at least comprises an output section, a lifting section and an input section which are connected with each other; the inlet of the output section is connected with the injection groove, and the outlet of the output section is connected with the inlet of the lifting section; the inlet of the input section is connected with the outlet of the lifting section, and the outlet of the input section is connected with the reaction tube cavity; the output section is connected with the lifting section at an angle, and the lifting section is connected with the input section at an angle, and the distance between the inlet of the lifting section and the reference plane is greater than or less than the distance between the outlet of the lifting section and the reference plane. The catheter-type PCR reaction tube of the present invention uses capillary phenomenon to evenly distribute and perfuse the sample solution when the sample solution passes through the output section, the lifting section and the input section.

Description

Technical Field

[0001] The present invention relates to the field of biomedical science and technology, and in particular to a catheter-type PCR reaction tube. Background Art

[0002] Conventional catheter-type PCR reaction tubes are equipped with multiple reaction cavities, and the sample solutions used for the experiment are all completed in the reaction cavities for chain reaction. Since the sample solution is usually injected into the reaction cavity for the experiment, it is inevitable that bubbles will remain in the sample solution in the reaction cavity. And the bubbles will affect the experimental results. Summary of the invention

[0003] The object of the present invention is to provide a catheter-type PCR reaction tube, which can avoid the generation of residual bubbles during the operation of inputting a sample solution into the reaction tube cavity.

[0004] The present invention is achieved in that:

[0005] A catheter-type PCR reaction tube, comprising a reaction tube base; a reference plane is arranged on the reaction tube base, an injection groove is arranged on the reference plane, and a plurality of reaction tube cavities located around the injection groove are also arranged on the reaction tube base, and any of the reaction tube cavities is connected to the injection groove through a guide tube; any of the guide tubes at least comprises an output section, a lifting section and an input section that are connected to each other; the inlet of the output section is connected to the injection groove, and the outlet of the output section is connected to the inlet of the lifting section; the inlet of the input section is connected to the outlet of the lifting section, and the outlet of the input section is connected to the reaction tube cavity; the output section is connected to the lifting section at an angle, and the lifting section is connected to the input section at an angle, and the distance between the inlet of the lifting section and the reference plane is greater than or less than the distance between the outlet of the lifting section and the reference plane.

[0006] Before using the above-mentioned catheter-type PCR reaction tube for polymerase chain reaction, the sample solution is first dripped into the injection groove. Since the sample solution temporarily stays in the injection groove, the bubbles generated in the dripping process float up and detach from the sample solution under the action of buoyancy, and only a few small bubbles remain in the sample solution; then, the sample solution flows from the injection groove through the output section, the lifting section and the input section of the guide tube. Since the multiple sections of the guide tube are connected at an angle, the sample solution uses capillary phenomenon to evenly distribute and infuse the sample solution when flowing through the lifting section.

[0007] It should be noted that, preferably, the position close to the reaction lumen on the reference plane should be marked or engraved with the serial number corresponding to the reaction lumen: 1, 2, 3, 4, etc. The serial number can also be Roman numerals Ⅰ, Ⅱ, Ⅲ, Ⅳ, etc., and the number of numbers corresponding to the number of reaction lumens should be marked.

[0008] Furthermore, the inlet of the lifting section is connected to the output section, and the outlet of the lifting section is connected to the input section. The technical effect is that in this structure, the sample solution first flows from the output section far from the reference plane to the lifting section, penetrates into the input section through capillary phenomenon in the lifting section, and finally flows into the reaction tube cavity.

[0009] Furthermore, the output section and the input section are parallel to the reference plane, and the lifting section is perpendicular to the reference plane. The technical effect is that it is conducive to accurate positioning and improves production efficiency.

[0010] Furthermore, the output section and the input section are parallel or perpendicular to each other. The technical effect is that when the size of the reaction tube base is large enough, the output section and the input section can be arranged in parallel with each other, which is conducive to the flow of the sample solution and is more conducive to the production of the reaction tube base. When the size of the reaction tube base is small, the output section can be arranged along the radial direction of the reaction tube base, and the input section can be arranged perpendicular to the radial direction of the reaction tube base, so as to ensure that enough reaction tube cavities and guide tubes are arranged in a small space.

[0011] Furthermore, the calibers of the output section and the input section are both larger than the caliber of the lifting section. The technical effect is that in this structure, the sample solution flowing out of the injection tank first passes through the output section with a larger caliber, and then enters the lifting section with a smaller caliber. According to the capillary phenomenon, the sample solution rises and penetrates into the input section, thereby achieving uniform distribution of the sample solution.

[0012] Furthermore, the connection port between the injection slot and the guide tube is located on the side of the injection slot away from the reference plane. The technical effect is that when the reaction tube base of this structure is placed horizontally on the reference plane, the injection slot opening faces upward, while the connection port between the injection slot and the guide tube faces downward. When injecting the sample solution, the bubbles generated during the liquid dripping process are not easy to enter the guide tube under the action of buoyancy, thereby improving the gas filtering effect of the reaction tube base.

[0013] Furthermore, the opening of the injection slot is smaller than its inner diameter. The technical effect is that the narrow opening can prevent the sample solution from spilling out of the injection slot, while the larger inner diameter can accommodate enough sample solution to meet the experimental requirements of multiple reaction cavities.

[0014] Furthermore, it also includes a plugging cover, which is arranged at the opening of the injection slot. Its technical effect is that during the experiment or when the equipment is stored, the plugging cover can block the injection slot to avoid unnecessary interference.

[0015] Furthermore, the plurality of reaction cavities are evenly distributed around the circumference of the injection slot. The technical effect is that the plurality of reaction cavities evenly distributed around the circumference are at equal distances from the injection slot, and the guide tubes arranged on this basis are also equidistant and have the same structure, so that the sample solution can be injected into the plurality of reaction cavities in equal amounts without having to design a different guide tube for each reaction cavity.

[0016] Furthermore, the circumferential outer edge of the reaction tube base is provided with threads. Its technical effect is that when the reaction tube base is conducting a chain reaction experiment, it needs to be fixedly placed in a specific environment such as a constant temperature or variable temperature environment. The threaded structure can be used for fixed connection. And when multiple reaction tube cavities are evenly distributed around the injection groove, there is no need to consider the location of a single reaction tube cavity.

[0017] The beneficial effects of the present invention are:

[0018] In the catheter-type PCR reaction tube of the present invention, when the sample solution introduced from the injection tank passes through the guide tube, the guide tube is provided with an output section, a lifting section and an input section connected at an angle, so that the sample solution can be evenly distributed and perfused by using capillary phenomenon. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0020] Figure 1 This is a structural diagram of the appearance of the catheter-type PCR reaction tube provided by the present invention;

[0021] Figure 2 The catheter-type PCR reaction tube provided in the first embodiment and the second embodiment of the present invention is Figure 1 AA section view in the figure;

[0022] Figure 3 The catheter-type PCR reaction tube provided in the third embodiment of the present invention is Figure 1 AA section view in the figure;

[0023] Figure 4 The catheter-type PCR reaction tube provided in the fifth embodiment of the present invention is Figure 1 AA section view in.

[0024] In the figure: 100 - reaction tube base; 101 - reference plane; 110 - injection groove; 120 - guide tube; 121 - output section; 122 - lifting section; 123 - input section; 130 - reaction tube cavity; 140 - thread. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations.

[0026] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0028] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, or the positions or positional relationships in which the product of the invention is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0029] In addition, the terms "horizontal", "vertical", "overhanging" and the like do not mean that the components are required to be absolutely horizontal or overhanging, but can be slightly tilted. For example, "horizontal" only means that its direction is more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0030] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0031] Some embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.

[0032] First embodiment:

[0033] Figure 1 This is a structural diagram of the appearance of the catheter-type PCR reaction tube provided by the present invention; Figure 2 The catheter-type PCR reaction tube provided in the first embodiment and the second embodiment of the present invention is Figure 1 Please refer to the AA section view in Figure 1 , Figure 2 The present embodiment provides a catheter-type PCR reaction tube, which includes a reaction tube base 100; a reference plane 101 is provided on the reaction tube base 100, an injection groove 110 is provided on the reference plane 101, and a plurality of reaction tube cavities 130 located around the injection groove 110 are also provided on the reaction tube base 100, and any reaction tube cavity 130 is connected to the injection groove 110 through a guide tube 120.

[0034] Each guide tube 120 at least includes an output section 121, an elevating section 122 and an input section 123 which are connected to each other; the inlet of the output section 121 is connected to the injection tank 110, and the outlet of the output section 121 is connected to the inlet of the elevating section 122; the inlet of the input section 123 is connected to the outlet of the elevating section 122, and the outlet of the input section 123 is connected to the reaction tube cavity 130;

[0035] The output section 121 is connected to the lifting section 122 at an angle, and the lifting section 122 is connected to the input section 123 at an angle. The distance between the entrance of the lifting section 122 and the reference plane 101 is greater than or less than the distance between the exit of the lifting section 122 and the reference plane 101.

[0036] The working principle and usage of the catheter PCR reaction tube are:

[0037] First, the sample solution is dripped into the injection groove 110. Since the sample solution temporarily stays in the injection groove 110, the bubbles generated in the dripping process float up and separate from the sample solution under the action of buoyancy, and only a few small bubbles remain in the sample solution. Then, the sample solution flows from the injection groove 110 to the output section 121, the lifting section 122 and the input section 123 of the guide tube 120. When the sample solution flows through the lifting section 122, it is easy to use the capillary phenomenon to evenly distribute and infuse the sample solution.

[0038] In addition, preferably, the position near the reaction tube cavity 130 on the reference plane 101 should be marked or engraved with the serial number corresponding to the reaction tube cavity 130: 1, 2, 3, 4, etc. The serial number can also be Roman numerals Ⅰ, Ⅱ, Ⅲ, Ⅳ, etc., and the number of numbers corresponding to the number of reaction tube cavities 130 should be marked.

[0039] Second embodiment:

[0040] Figure 1 This is a structural diagram of the appearance of the catheter-type PCR reaction tube provided by the present invention; Figure 2 The catheter-type PCR reaction tube provided in the first embodiment and the second embodiment of the present invention is Figure 1 Please refer to the AA section view in Figure 1 , Figure 3 The present embodiment provides a catheter-type PCR reaction tube, which is substantially the same as the catheter-type PCR reaction tube of the first embodiment. The difference between the two is that the inlet of the lifting section 122 of the catheter-type PCR reaction tube of the present embodiment is connected to the output section 121, and the outlet of the lifting section 122 is connected to the input section 123.

[0041] Third embodiment:

[0042] Figure 1 This is a structural diagram of the catheter-type PCR reaction tube provided by the present invention. Figure 3 The catheter-type PCR reaction tube provided in the third embodiment of the present invention is Figure 1 Please refer to the AA section view in Figure 1 , Figure 4 This embodiment provides a catheter-type PCR reaction tube, which is substantially the same as the catheter-type PCR reaction tube of the first embodiment or the second embodiment. The difference between the two is that the output section 121 and the input section 123 of the catheter-type PCR reaction tube of this embodiment are parallel to the reference plane 101, and the lifting section 122 is perpendicular to the reference plane 101.

[0043] In this embodiment, the structure in which the output section 121, the lifting section 122 and the input section 123 are interconnected can be set to be repeated in multiples to form multiple filtering.

[0044] Fourth embodiment:

[0045] This embodiment provides a catheter-type PCR reaction tube, which is substantially the same as the catheter-type PCR reaction tube of the first embodiment, the second embodiment or the third embodiment. The difference between the two is that in the catheter-type PCR reaction tube of this embodiment, the output section 121 and the input section 123 are perpendicular to each other.

[0046] Optionally, the output section 121 and the input section 123 may also be arranged in parallel with each other, which is beneficial for the sample solution to flow smoothly and is more conducive to the production of the reaction tube base 100 .

[0047] In this embodiment, the output section 121 and the input section 123 are perpendicular to each other, that is, the output section 121 connected to the injection slot 110 can be arranged along the radial direction of the reaction tube base 100, and the input section 123 connected to the lifting section 122 and the reaction tube cavity 130 can be arranged perpendicular to the radial direction of the reaction tube base 100. This structure can ensure that enough reaction tube cavities 130 and guide tubes 120 are arranged in a small space.

[0048] On the basis of any of the above embodiments, further, the diameter of the output section 121 and the diameter of the input section 123 are both larger than the diameter of the lifting section 122 .

[0049] Fifth embodiment:

[0050] Figure 1 This is a structural diagram of the catheter-type PCR reaction tube provided by the present invention. Figure 4 The catheter-type PCR reaction tube provided in the fifth embodiment of the present invention is Figure 1 Please refer to the AA section view in Figure 1 , Figure 4 This embodiment provides a catheter-type PCR reaction tube, which is substantially the same as the catheter-type PCR reaction tube of the first embodiment, the second embodiment, the third embodiment or the fourth embodiment. The difference between the two is that in the catheter-type PCR reaction tube of this embodiment, the connection port between the injection groove 110 and the guide tube 120 is located on the side of the injection groove 110 away from the reference plane 101.

[0051] Furthermore, if Figure 4 As shown, the opening of the injection groove 110 is smaller than its inner diameter. Optionally, the injection groove 110 may also be a cylindrical groove cavity, or the injection groove 110 may also be a spherical groove cavity structure.

[0052] In the catheter-type PCR reaction tube of the above embodiment, when the reference plane 101 is placed horizontally, the injection slot 110 opens upward, and the connection port between the injection slot 110 and the guide tube 120 faces downward. When injecting the sample solution, bubbles generated during the liquid dripping process are not easy to enter the guide tube 120 due to the buoyancy, thereby improving the gas filtering effect of the reaction tube base 100.

[0053] On the basis of any of the above embodiments, further, a plugging cover is included; the plugging cover is arranged at the opening of the injection groove 110. The plugging cover can be arranged at the opening of the injection groove 110, or can be arranged on the entire reference plane 101, so as to uniformly block the injection groove 110 and the reaction tube cavity 130.

[0054] On the basis of any of the above embodiments, further, Figure 1As shown, the plurality of reaction cavities 130 are evenly distributed around the circumference of the injection slot 110. Optionally, the plurality of reaction cavities 130 can also be distributed in a determinant in the reaction tube base 100 to match the experimental support of the reaction tube. When the plurality of reaction cavities 130 are distributed in a determinant, it is difficult to inject the sample solution into each reaction cavity 130 in equal amounts and simultaneously.

[0055] On the basis of any of the above embodiments, further, Figure 4 As shown, the circumferential outer edge of the reaction tube base 100 is provided with a thread 140. When the reaction tube base 100 is used for chain reaction experiments, it needs to be fixedly placed in a specific environment such as a constant temperature or variable temperature environment. The thread 140 structure can be used for fixed connection. When multiple reaction tube cavities 130 are evenly distributed around the injection groove 110, there is no need to consider the position of a single reaction tube cavity 130.

[0056] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A catheter-type PCR reaction tube, characterized in that: The invention comprises a reaction tube base (100); a reference plane (101) is arranged on the reaction tube base (100); an injection groove (110) is arranged on the reference plane (101); a plurality of reaction tube cavities (130) are also arranged on the reaction tube base (100) and are located around the injection groove (110); any one of the reaction tube cavities (130) is connected to the injection groove (110) via a guide tube (120); A connection port between the injection groove (110) and the guide tube (120) is located on a side of the injection groove (110) away from the reference plane (101); Any of the guide tubes (120) comprises at least an output section (121), an elevating section (122), and an input section (123) which are interconnected; the inlet of the output section (121) is connected to the injection tank (110), and the outlet of the output section (121) is connected to the inlet of the elevating section (122); the inlet of the input section (123) is connected to the outlet of the elevating section (122), and the outlet of the input section (123) is connected to the reaction tube cavity (130); The output section (121) is connected to the lifting section (122) at an angle, the lifting section (122) is connected to the input section (123) at an angle, and the distance between the inlet of the lifting section (122) and the reference plane (101) is greater than the distance between the outlet of the lifting section (122) and the reference plane (101); The output section (121) and the input section (123) are both parallel to the reference plane (101), the lifting section (122) is perpendicular to the reference plane (101), and the lifting section (122) utilizes capillary phenomena to evenly distribute and perfuse the sample solution.

2. The catheter-type PCR reaction tube according to claim 1, characterized in that: The output section (121) and the input section (123) are parallel to each other.

3. The catheter-type PCR reaction tube according to claim 1, characterized in that: The caliber of the output section (121) and the caliber of the input section (123) are both larger than the caliber of the lifting section (122).

4. The catheter-type PCR reaction tube according to claim 1, characterized in that: The opening of the injection groove (110) is smaller than its inner diameter.

5. The catheter-type PCR reaction tube according to claim 1, characterized in that: It also comprises a plugging cover; the plugging cover is arranged at the opening of the injection groove (110).

6. The catheter-type PCR reaction tube according to claim 1, characterized in that: The plurality of reaction cavities (130) are evenly distributed around the circumference of the injection groove (110).

7. The catheter-type PCR reaction tube according to claim 1, characterized in that: The circumferential outer edge of the reaction tube base (100) is provided with a thread (140).

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