Pertussis filamentous hemagglutinin fusion protein and application thereof

A fusion protein, pertussis technology, applied in the direction of immunoglobulin, antibacterial immunoglobulin, hybrid peptide, etc., can solve problems such as child death

CN111333734AActive Publication Date: 2020-06-26ACADEMY OF MILITARY MEDICAL SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2020-06-26

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Abstract

The invention relates to a pertussis filamentous hemagglutinin fusion protein and application thereof. The fusion protein is formed by connecting pertussis FHA (pertussis filamentous) amino acid residue 1877-2250 fragments with StxB (shiga toxin B subunit) through a peptide chain, a subunit vaccine is constructed by the fusion protein, inducible expression is successfully carried out in escherichia coli, and the immunogenicity of the fusion protein is authenticated through Western-blot and ELISA (enzyme-linked immuno sorbent assay). After a mouse is immunized, a discovery result shows that a high-level antibody valence can be generated, and a new through is provided for researching a filamentous hemagglutinin vaccine.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a pertussis filamentous hemagglutinin fusion protein and its application. Background technique

[0002] Pertussis, an acute respiratory infectious disease caused by Bordetella pertussis, is one of the relatively new human infectious diseases. The disease, which first appeared in France in 1414, is one of the world's worst preventable diseases with vaccines. Pertussis is clinically characterized by paroxysmal or explosive multiple rapid coughs, accompanied by a characteristic "chicken crowing"-like inspiratory roar, and the most common complication is secondary pneumonia. The disease is more common in children and infants, especially those younger than 6 months. Despite the availability of a preventive vaccine against pertussis and high vaccination rates, pertussis remains one of the leading causes of death in children.

[0003] Natural filamentous hemagglutinin (filamentous haemag...

Examples

Embodiment 1

[0069] Embodiment 1 prevents the preparation of animal pertussis vaccine

[0070] The present invention relates to the gene of 3 proteins, respectively the nucleotide sequence is His-StxB-Fha of SEQ ID No.1 1877-2250 Gene (coding amino acid sequence is the protein of SEQ ID No.2

[0071] His-StxB-Fha 1877-2250 ), the nucleotide sequence is the StxB-Fha of the 109-1509th position of SEQ ID No.1 1877-2250 Gene (the coding amino acid sequence is the protein StxB-Fha of the 37th-503rd position of SEQ ID No.2 1877-2250 ) and His-Fha as a control 1877-2250 Gene (coding protein His-Fha 1877-2250 ).

[0072] SEQ ID No.1 consists of 1536 nucleotides, the 1-108th is the sequence on pET28a(+), the 109-378th is the StxB gene, the 379-390th is the connecting peptide gene, and the 391-1509th Fha 1877-2250 Gene, 1510-1536 is the sequence on pET28a(+). a) The gene name is His-StxB-Fha 1877-2250 Gene, the gene name of b) is StxB-Fha 1877-2250 Gene.

[0073] Among them, SEQ ID No.2 c...