A potato aseptic fog cultivation test device

Abstract

The present invention discloses a potato sterile fog cultivation test device, which includes a seedling observation device, a nutrient solution storage device, a nutrient solution atomization device and a black light-shielding sleeve bag. The seedling observation device includes a tissue culture bottle cap and a transparent glass bottle, and the bottom of the transparent glass bottle is a light-shielding seedling tray; the nutrient solution storage device is a transparent glass bottle and is located below the seedling observation device; a switchable liquid storage and liquid discharging device is provided at the bottom of the transparent glass bottle; the nutrient solution atomization device includes a liquid storage bottle and a liquid atomizer, and the black light-shielding sleeve bag can be sleeved outside the nutrient solution storage device and the nutrient solution atomization device for light shielding. Through the experimental device and method of the present invention, various tests related to potatoes can be carried out in the laboratory. The advantages of this device are: it can ensure sterility, the test conditions are controllable, morphological observation is easy at different developmental stages, sampling is convenient, the replacement of sterile nutrient solution can be carried out, it is portable for laboratory use, the investment cost is low, and the operability is strong.

Description

Technical Field

[0001] The present invention belongs to the technical field of potato aeroponics, and particularly relates to a potato aseptic aeroponics test device. Background Art

[0002] Potato (Solanum tuberosum L.) is the fourth major food and vegetable crop in the world after rice, wheat, and corn. Potato is a typical plant with asexual reproduction by tubers. Its tubers are the main yield-forming organs and reproductive organs. Tuber formation is the result of the swelling of the tip of the underground stolon. Morphologically, it is divided into four stages: the occurrence and elongation period of stolons, the apical swelling period of stolons, the initial stage of tuber formation, and the mature stage of tubers. This complex process of tuber formation is accompanied by morphological construction and material accumulation, and is regulated by environmental factors.

[0003] Tissue culture technology is very important in potato germplasm preservation, the cultivation and propagation of potato virus-free plants, and the research on potato tuber formation and development, and is an important technology to support potato scientific research.

[0004] Aeroponic cultivation of virus-free potato minitubers is a new cultivation mode, and its efficiency is dozens of times that of substrate cultivation. This technology has changed the traditional cultivation mode, improved production efficiency, greatly accelerated the production speed of virus-free potato minitubers, and under certain conditions, it can also achieve annual production. It is an important development trend for the production of virus-free potato minitubers and an important cultivation mode for future agricultural production. At present, aeroponic technology is mainly applied to the production of virus-free potato minitubers by small and medium-sized enterprises. By spraying nutrient solution on the rhizosphere of potato plants kept in the dark, the potato can obtain sufficient nutrition to promote the growth of potato tubers. Only when the plants are in relatively suitable nutrient solution conditions can they obtain higher yields and qualities. Nutrient solution is the core of aeroponic cultivation. According to the requirements of plant growth and development for mineral elements, appropriate nutrient solution should be sprayed in a timely and appropriate amount to maximize the production potential and improve the production efficiency of aeroponic cultivation.

[0005] However, in industrial production, it is difficult to explore problems such as the optimal ratio of nutrient solution involved in aeroponic technology through experiments. There are problems such as uncontrollable conditions, large investment in manpower and material resources, and possible waste in production, which increase the production cost of enterprises and are not conducive to the development of enterprises. Therefore, if the industrial aeroponic technology can be integrated into controllable laboratory conditions by using laboratory tissue culture technology, the research cost of enterprises can be greatly reduced, and the problem of continuous observation in the basic research on potato tuber formation and development in the laboratory can also be solved.

[0006] By combining tissue culture technology and aeroponics technology under laboratory conditions, the enterprise can reduce the cost of optimizing the aeroponics technology for potatoes, and the laboratory can solve the problem of continuous cultivation of materials in the study of scientific issues such as potato tuber development. The key to this technology is to maintain a sterile environment for the growth of potato tissue culture plants from stem segments to the swelling and tuber formation of the whole plant in the laboratory, and no seedling transplantation is required during the whole process, ensuring that the whole process can be observed and various treatment experiments can be carried out. So far, there is no such device or method to achieve this process. If such a device is to be made, there are the following difficulties: how to ensure a sterile environment for the device; how to promote plant growth with liquid nutrient solution in the early stage and switch to an atomized form to promote tuber formation in the later stage; how to achieve full light transmission in the early stage and upper part light and lower part darkness during tuber formation in the later stage; how to ensure that the whole process can be observed; how to replace the nutrient solution in a sterile environment in the later stage. All these problems need to be solved.

[0007] Problems in the prior art: There is no specially designed aeroponics device for laboratory sterile conditions in the prior art. The common aeroponics devices are mainly large-scale devices for enterprise production and cannot meet the laboratory requirements. Moreover, in the study of basic scientific issues such as the tuber formation and development process in the laboratory, pot experiments or in vitro culture are mainly selected. However, it is rather difficult to observe the underground tuber formation process through pot experiments; in vitro culture experiments cannot observe the complete process of whole-plant tuber formation and development. Therefore, this patent aims to develop a potato aeroponics device for scientific research in the laboratory, which can well solve the problems of uncontrollability and high cost in the optimization process of aeroponics technology, and at the same time provide a convenient, controllable, sterile and easy-to-observe method for cultivating experimental materials for basic scientific research on potatoes. Summary of the Invention

[0008] To solve the above technical problems, the present invention provides a potato sterile aeroponics test device, which includes a seedling observation device, a nutrient solution storage device, a nutrient solution atomization device and a black light-shielding sleeve. The seedling observation device includes a tissue culture bottle cap and a light-transmitting glass bottle. The bottom of the light-transmitting glass bottle is a light-shielding seedling tray, and there are seedling tray planting holes on the light-shielding seedling tray; the nutrient solution storage device is a light-transmitting glass bottle and is located below the seedling observation device; a switchable liquid storage and liquid discharging device is provided at the bottom of the light-transmitting glass bottle; the nutrient solution atomization device includes a liquid storage bottle and a liquid atomizer, and the liquid atomizer is located inside the liquid storage bottle; the nutrient solution atomization device is located below the nutrient solution storage device, and the upper end is threadedly connected to the nutrient solution storage device; an atomization tray is provided at the bottom of the nutrient solution storage device, and air spraying holes are evenly distributed on the surface of the atomization tray. The atomization tray is connected to the atomizer through a gas guiding hose; the black light-shielding sleeve can be sleeved outside the nutrient solution storage device and the nutrient solution atomization device for light shielding.

[0009] Preferably, the tissue culture bottle cap is threadedly connected to the light-transmitting glass bottle.

[0010] Preferably, the upper end of the nutrient solution storage device is threadedly connected to the seedling observation device.

[0011] Preferably, the switchable liquid storage and liquid discharging device is a liquid outlet pipe connecting the nutrient solution storage and the atomization device, and an electromagnetic valve I is provided on the liquid outlet pipe.

[0012] Preferably, a temperature sensor is provided on the inner wall of the nutrient solution storage device.

[0013] Preferably, a heating box 11 is further provided between the atomizer and the atomization disk. The heating component in the heating box 11 is a resistance wire, and the resistance wire is connected to the temperature control module provided on the control panel.

[0014] Preferably, the electromagnetic valve I and the temperature sensor are respectively connected to the control panel.

[0015] Preferably, the atomizer is a pulsed liquid atomizer.

[0016] Preferably, an electromagnetic valve II is provided on the air guide hose.

[0017] Preferably, the electromagnetic valve II is connected to the control panel.

[0018] Through the experimental device and method of the present invention, various tests related to potatoes can be carried out in the laboratory. The advantages of this device are: it can ensure sterility, the test conditions are controllable, morphological observations are easy to conduct at different developmental stages, sampling is convenient, the replacement of sterile nutrient solution can be carried out, it is portable for laboratory use, the input cost is low, and the operability is strong. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The following is further described in conjunction with the drawings and specific embodiments:

[0020] Figure 1 This is the elevation view of the potato sterile mist cultivation test device of the present invention.

[0021] Figure 2 This is the perspective view of the potato sterile mist cultivation test device of the present invention.

[0022] Figure 3 This is the structural schematic diagram of the seedling observation device.

[0023] Figure 4 This is the structural schematic diagram of the nutrient solution storage device.

[0024] Figure 5 This is the structural schematic diagram of the nutrient solution atomization device.

[0025] Figure 6 This is the structural schematic diagram of the black light-shielding sleeve bag.

[0026] Figure 7 It is a schematic structural diagram of an aerosol generating device. Specific embodiments

[0027] As Figure 1-7 shown, the present invention discloses a potato aseptic mist cultivation test device:

[0028] Structure A: It is a seedling observation and cultivation device with a black light-shielding seedling tray with holes (for inserting stem segments) at the bottom. 1 is the tissue culture bottle cap, which has a breathable membrane for filtering bacteria in the middle and can be screwed tightly onto 2. 2 is a light-transmitting glass bottle, which is convenient for plant photosynthesis and observation. 3 is the seedling tray planting hole, which mainly fixes the stem segments. The height of the light-transmitting glass bottle is 200 mm, and the diameter is 250 mm. 4 is the light-shielding seedling tray, which plants the plants and prevents light from penetrating from above into device B.

[0029] Structure B: A nutrient solution storage and potato tuber formation observation device. 5 is the screw connection port with device A. 6 is a light-transmitting glass bottle. 7 is the bottom cover of device B. The bottom of device B is provided with a liquid outlet pipe communicating with the nutrient solution storage and atomization device, and a solenoid valve I is provided on the liquid outlet pipe; the height of the nutrient solution storage device is 150 mm.

[0030] Structure C: A nutrient solution storage and atomization device. 8 is the screw connection port with device B. 9 is a liquid storage bottle. 10 is a liquid atomizer, which can automatically control the atomization frequency; the height of the nutrient solution atomization device is 150 mm.

[0031] The bottom of device B is provided with an atomization tray 13. The surface of the atomization tray is evenly distributed with air spray holes. The atomization tray is connected to the atomizer 10 through a gas guide hose 12. The atomizer can be a pulse liquid atomizer, and the atomizer is controlled by an electronic frequency controller.

[0032] A solenoid valve II is provided on the gas guide hose. A temperature sensor is provided on the inner wall of the nutrient solution storage and potato tuber formation observation device. The solenoid valve I, solenoid valve II, temperature sensor and atomizer are respectively connected to the control panel.

[0033] A heating box 11 is further provided between the atomizer 10 and the atomization tray 13. The heating component in the heating box 11 is a resistance wire, and the resistance wire is connected to the temperature control module provided on the control panel.

[0034] Structure D: A black light-shielding sleeve bag 14, which shields device B and C from light during the atomization start period to promote potato tuber formation.

[0035] The usage method of the potato aseptic mist cultivation test device of the present invention:

[0036] Optimization experiment of mist cultivation nutrient solution:

[0037] (1) Prepare the MS nutrient solution, pour it into Device B, connect it to Device A, tighten the upper cover of A, tighten the bottom cover of Device B, close Solenoid Valve I and Solenoid Valve II to make it in a completely airtight state, and place it in an autoclave for sterilization.

[0038] (2) Under a sterile environment, cut a potato stem segment about 7 cm long, cut off the leaves below the morphological part, and only retain 1 - 2 leaves above the morphological part; pass the stem segment through the small holes on the seedling tray so that its bottom touches the MS nutrient solution.

[0039] (3) Prepare nutrient solutions with different proportion formulas according to the experiment, add them at the same growth stage or different growth stages, observe the growth and tuber formation of potato plants with different formula nutrient solutions, obtain the optimal ratio of nutrient solution, optimize the fog cultivation conditions, and provide experimental data for the enterprise's fog cultivation technology.

[0040] Laboratory basic scientific research experiment:

[0041] (1) Prepare the MS nutrient solution, pour it into Device B, connect it to Device A, tighten the upper cover of A, tighten the bottom cover of Device B to make it in a completely airtight state, and place it in an autoclave for sterilization;

[0042] (2) Under a sterile environment, cut a potato stem segment about 6 cm long, cut off the leaves below the morphological part, and only retain 1 - 2 leaves above the morphological part; pass the stem segment through the small holes on the seedling tray so that its bottom touches the MS nutrient solution;

[0043] (3) Place Structure C on the ultra - clean workbench for sterilization with an ultraviolet lamp, then connect it (seamlessly) to Structures A and B, and place it in a light incubator for cultivation. At this time, Device B is filled with nutrient solution, the bottom cover 7 in B is in the closed state, and the nutrient solution does not flow into C;

[0044] (4) After the plants grow taller and take root for one week, open Solenoid Valve I, and the nutrient solution in B flows into C. B is in an empty state, which is convenient for observation and tuber formation. After the nutrient solution in B completely flows into C, close Solenoid Valve I, open Solenoid Valve II, turn on the atomization device of C, and set the atomization frequency.

[0045] The surface of the atomization disk 13 can also be covered with a rubber pad to block the air spray holes. The bottom of the rubber pad is provided with a protrusion adapted to the air spray holes. In this way, when the bottom cover 7 is in the closed state in step (3), the protrusion squeezes into the air spray holes, and the liquid does not enter the atomization disk. After the nutrient solution in B completely flows into C, uncover the rubber pad and turn on the atomization device for atomization.

[0046] (5) Put D on B and C to keep B and C in a completely dark state to promote the formation of stolons and tuber formation.

[0047] (6) At regular intervals, the tuber formation situation and atomization situation can be observed by opening the D refraction sleeve.

[0048] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A potato aseptic fog cultivation test device, characterized in that, It includes a seedling observation device, a nutrient solution storage device, a nutrient solution atomization device, and a black light-shielding sleeve bag. The seedling observation device includes a tissue culture bottle cap and a light-transmitting glass bottle. The bottom of the light-transmitting glass bottle is a light-shielding seedling tray, and there are seedling tray planting holes on the light-shielding seedling tray; the nutrient solution storage device is a light-transmitting glass bottle and is located below the seedling observation device; a switchable liquid storage and liquid discharging device is provided at the bottom of the light-transmitting glass bottle; the nutrient solution atomization device includes a liquid storage bottle and a liquid atomizer, and the liquid atomizer is located inside the liquid storage bottle; the nutrient solution atomization device is located below the nutrient solution storage device, and the upper end is threadedly connected to the nutrient solution storage device; a atomization tray is provided at the bottom of the nutrient solution storage device, and air spraying holes are evenly distributed on the surface of the atomization tray, and the atomization tray is connected to the atomizer through a gas guiding hose; The black light-shielding sleeve bag can be sleeved outside the nutrient solution storage device and the nutrient solution atomization device for light shielding; The tissue culture bottle cap is threadedly connected to the light-transmitting glass bottle; The upper end of the nutrient solution storage device is threadedly connected to the seedling observation device; The switchable liquid storage and liquid discharging device is a liquid outlet pipe connecting the nutrient solution storage and atomization devices, and a solenoid valve Ⅰ is provided on the liquid outlet pipe; A temperature sensor is provided on the inner wall of the nutrient solution storage device; A heating box is also provided between the atomizer and the atomization tray. The heating component in the heating box is a resistance wire, and the resistance wire is connected to a temperature control module provided on the control panel; The solenoid valve Ⅰ and the temperature sensor are respectively connected to the control panel; The surface of the atomization tray is covered with a rubber pad to block the air spraying holes.

2. The potato aseptic fog cultivation test device according to claim 1, characterized in that, The atomizer is a pulsed liquid atomizer.

3. The potato aseptic fog cultivation test device according to claim 1, characterized in that, A solenoid valve Ⅱ is provided on the gas guiding hose.

4. The potato aseptic fog cultivation test device according to claim 3, characterized in that, The solenoid valve Ⅱ is connected to the control panel.

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