A bactericidal mixture containing a triazole compound and its application

Abstract

The present invention relates to a bactericidal mixture containing a triazole compound and its application, which comprises active ingredient A and active ingredient B. Active ingredient A is a compound represented by formula (I), and active ingredient B is a triazole fungicide compound. The mixture or its preparation of the present invention is applied to wheat and has good preventive and therapeutic effects on wheat diseases.

Description

Technical Field

[0001] The present invention relates to the field of pesticide bactericides, and particularly relates to a bactericidal mixture containing a triazole compound and its application. The bactericidal mixture or its preparation can enhance the drug efficacy, reduce the dosage of the drug, and at the same time improve the control effect and delay the development of drug resistance. Background Art

[0002] Triazole bactericides use the triazole ring to coordinate with the central iron atom of the iron porphyrin in the fungal cell to hinder the formation of the iron porphyrin iron-oxygen complex, thereby inhibiting the synthesis of ergosterol, and ultimately causing the death of the fungal body due to damage to the cell membrane. Triazole bactericides have significant plant growth regulation characteristics.

[0003] Triazole bactericides can disrupt the balance between important plant hormones, regulate plant growth, and are even called "plant multi-protectants". Application practices have proved that triazole bactericides can effectively alleviate the adverse effects of various stress conditions such as salt stress, drought stress, freezing stress, and high temperature stress on the normal growth and metabolism of plants by regulating the physiological effects of plants. The unique antibacterial mechanism and broad bactericidal spectrum have made triazole bactericides widely used in agricultural production practices as mainstream pesticides for a long time. Triazole bactericides mainly include triadimefon, Triadimenol, uniconazole, Diniconazole, paclobutrazol, propiconazole, difenoconazole, tetraconazole, mefentrifluconazole, tebuconazole, myclobutanil, prothioconazole, etc.

[0004] Mefentrifluconazole is a C14-demethylation inhibitor in sterol biosynthesis. The unique isopropanol group in the mefentrifluconazole molecule enables it to very flexibly rotate from the free state to bind to the target to become the bound state, which can well inhibit the transfer of Septoria musiva, reduce the mutation of the pathogen, and delay the generation and development of resistance. The flexible and variable spatial form enables mefentrifluconazole to always maintain high efficiency against a variety of resistant strains, and it is a very excellent resistance management tool. It can be used for seed treatment or foliar spraying. CAS Registry Number: 494793-67-8, and its chemical structural formula is:

[0005]

[0006] Prothioconazole's mechanism of action is to inhibit the demethylation of the sterol precursor - lanosterol or 2,4 - methylenedihydrolanosterol at the 14th position in fungi, and it has good systemic activity. It is mainly used for cereals, soybeans, rapeseed, rice, peanuts, beets, vegetables, etc. It has a broad bactericidal spectrum and has excellent control effects on almost all fungal diseases of cereals, including diseases caused by Septoria spp., Fusarium spp., Rhynchosporium spp., etc., such as powdery mildew, scab, sheath blight, rust, glume blotch, leaf spot, net blotch, sclerotinia, basal rot, mold, etc. Prothioconazole can be used for both foliar spraying and seed treatment; it is safe for humans and the environment. Its chemical structural formula is:

[0007]

[0008] The compound shown in formula (I) is a broad - spectrum pyridine fungicide (Aminopyrifen) developed by Japanese Agro - kanesho Corporation, with the development code AKD - 5195. Its IUPAC chemical name is: 4 - phenoxybenzyl 2 - amino - 6 - methylnicotinate, and the CAS registration number is: 1531626 - 08 - 0. This compound has relatively high control effects on powdery mildew and gray mold of various fruit and vegetable crops. Its chemical structural formula is as shown in formula (I):

[0009]

[0010] Diseases of food crops severely restrict the safe production of crops. The occurrence of diseases will not only cause a large reduction in crop yields but also seriously affect the quality of crops. The use of fungicides is the most economical and effective means for plant disease control and plays an important role in agricultural production. Long - term continuous high - dose use of a single chemical agent is likely to cause a series of problems such as the generation of drug resistance in harmful bacteria and environmental pollution. Rational compounding or mixing of chemical agents has positive characteristics such as expanding the bactericidal spectrum, improving the control effect, extending the suitable application period of pesticides, reducing the amount of pesticides used, reducing phytotoxicity, reducing residues, and delaying the occurrence of drug resistance and resistance in harmful bacteria. As farmers' awareness of environmental protection concepts deepens, high - efficiency, low - toxicity, high - activity, and low - residue have become an inevitable trend in the development of pesticides. The present invention has conducted in - depth research on the application of Aminopyrifen, cyflufenamid, and prothioconazole to fungal diseases. A large number of test results show that reasonable compounding of Aminopyrifen with cyflufenamid and prothioconazole can effectively improve the control effect. With the increasing requirements for the environment and food safety, as well as the resistance problem of pesticides, how to use pesticides scientifically, reduce the amount of chemical pesticides used, and improve the efficacy of pesticides has become an urgent problem in the field of pesticides. Summary of the Invention

[0011] Based on the above situation, the object of the present invention is to provide a bactericidal mixture containing triazole compounds and its preparation, which is mainly used for preventing and controlling plant fungal diseases. The composition or its preparation can enhance the efficacy, reduce the dosage of drugs, and at the same time can extend the effective period and delay the development of drug resistance.

[0012] To achieve the above object, a bactericidal mixture containing triazole compounds comprises active ingredient A as Aminopyrifen:

[0013] and active ingredient B as a triazole fungicide compound.

[0014] Further, the triazole fungicide compound is selected from cyflufenamid and prothioconazole.

[0015] Further, the mass ratio of active ingredient A to active ingredient B is 50:1 to 1:50.

[0016] Further, the mass ratio of Aminopyrifen to cyflufenamid is 15:1 to 1:30; preferably, the mass ratio is 5:1 to 1:10.

[0017] Further, the mass ratio of Aminopyrifen to cyflufenamid is 15:1, 10:1, 5:1, 1:1, 1:5, 1:10, 1:15 or 1:30.

[0018] Further, the mass ratio of Aminopyrifen to prothioconazole is 4:1 to 1:20; preferably, the mass ratio is 4:1 to 1:8.

[0019] Further, the mass ratio of Aminopyrifen to prothioconazole is 4:1, 2:1, 1:2, 1:4, 1:8 or 1:20.

[0020] Further, based on the total weight of the bactericidal mixture being 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the bactericidal mixture is 1 to 95 wt%, preferably 5 to 80 wt%, more preferably 10 to 60 wt%.

[0021] Specifically, the sum of the contents of Aminopyrifen and cyflufenamid in the bactericidal mixture is 10 to 60 wt%, for example, the sum of the contents of Aminopyrifen and cyflufenamid is 24 wt%, 30 wt%, 35 wt% and 40 wt%;

[0022] Alternatively, the sum of the contents of Aminopyrifen and prothioconazole in the bactericidal mixture is 10 to 60 wt%, for example, the sum of the contents of Aminopyrifen and prothioconazole is 20 wt%, 25 wt%, 30 wt% and 35 wt%.

[0023] A bactericidal mixture containing a triazole compound provided by the present invention, the bactericidal mixture further includes an adjuvant, and the adjuvant is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze, an antifoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier.

[0024] The wetting agent is selected from one or more of alkyl benzene sulfonates, alkyl naphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, fatty alcohol polyoxyethylene ether sulfates, silkworm excrement, soap pod powder, sapindus powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0025] The dispersant is selected from one or more of lignin sulfonates, alkyl naphthalene sulfonate formaldehyde condensates, naphthalene sulfonates, triphenylvinylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensate sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0026] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styryl phenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0027] The thickener is selected from one or more of xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and silica white; and / or

[0028] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0029] The antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0030] The antifoaming agent is selected from C 10 -C 20Saturated fatty acid compounds, silicone oils, silicone compounds, C 8 -C 10 One or more of fatty alcohols; and / or

[0031] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent naphtha, vegetable oil (such as soybean oil, corn oil, rapeseed oil, palm oil, etc.), vegetable oil derivatives and water; and / or

[0032] The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon and 1,2-benzisothiazolin-3-one; and / or

[0033] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, diatomaceous earth, bentonite, attapulgite, silica white, talc powder, montmorillonite and starch; and / or

[0034] The synergist is selected from synergistic phosphorus, synergistic ether; and / or

[0035] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0036] The present invention provides a bactericidal mixture containing a triazole compound and its application. The dosage form of the bactericidal mixture can be any one of emulsifiable concentrate, emulsion in water, microemulsion, suspension concentrate, dispersible oil suspension, wettable powder or water dispersible granule;

[0037] Furthermore, the dosage form of the preparation is any one of wettable powder, suspension concentrate, dispersible oil suspension.

[0038] Emulsifiable concentrate is a liquid prepared by dissolving a high concentration of active ingredient in a solvent and adding an emulsifier. Most emulsifiers use a mixture of non-ionic and anionic surfactants. The preparation of emulsifiable concentrate is relatively simple and convenient, and the biological effect is relatively high. However, the raw material cost is high, and the cost of the preparation is too high, increasing the manufacturing cost of the preparation.

[0039] Emulsion in water is a preparation in which liquid pesticide technicals, which are liquid or prepared by mixing with a solvent, are dispersed in water in the form of small droplets with a size of 0.5 - 1.5 microns, and the appearance is a milky white liquid. In addition to the active ingredient and the solvent, emulsion in water also adds auxiliary agents such as emulsifiers, dispersants, antifreezing agents, etc. to improve the stability of the emulsion.

[0040] Microemulsion is composed of liquid pesticides, dispersants, water, stabilizers, etc. The pesticide particles are 0.01 - 0.1 micrometers. With water as the medium and containing little or no organic solvents, it has little environmental pollution, strong permeability to target organisms, and good adhesion. It is an environmentally friendly green pesticide formulation.

[0041] A suspension concentrate refers to a composition mixed with a suitable surfactant, water or organic solvent in a certain proportion, and then pulverized to D 90 (The particle size of 90% of the particles) < 10 μm to obtain a pulverized slurry, and then thickeners, dispersants, anti-settling agents, defoamers, antifreeze agents, deionized water, etc. are added to the pulverized slurry and mixed. Suspension concentrates are divided into two types: water suspension concentrates and oil suspension concentrates. They have small particle sizes, high biological activity, no problem of dust flying, and are not flammable or explosive.

[0042] A dispersible oil suspension concentrate is a liquid formulation in which the active ingredient (possibly containing other solvents) is stably suspended in an organic fluid and is used after dilution with water. Compared with other dosage forms, the dispersible oil suspension concentrate has the characteristics of safety, environmental protection, and good drug properties. During the production process of the dispersible oil suspension concentrate, there is no dust pollution, and no flammable or explosive organic solvents are used, which is relatively safe for producers and users, and is also convenient and safe for storage and transportation, and is relatively safe for the environment.

[0043] A wettable powder refers to the original drug of the composition being pre-pulverized and mixed evenly with a dispersant, wetting agent, filler, etc. in a certain proportion, and then pulverized by an air-flow pulverizer until the fineness meets the requirement of at least 98 wt% passing through a 45 μm test sieve. A wettable powder is a formulation that can be evenly dispersed in water. In addition to the active substance and inert substance, it also contains a certain amount of anionic or non-ionic surfactants. Wettable powders do not use solvents and emulsifiers, are relatively safe for plants, are not prone to causing phytotoxicity, and are environmentally safe.

[0044] A water dispersible granule is to regranulate a wettable powder or a suspension concentrate into a water dispersible granule. The composition is mixed with a suitable surfactant and inert substance powder in a certain proportion to form a mixture, and then granulated by methods such as fluidized bed granulation, spray granulation, or pan granulation. During the mixing process of the raw materials, air-flow pulverization is carried out to make the fineness of the powder particles meet the requirements, and a double helix mixer and a plow knife mixer are used for multiple mixings to make the product evenly mixed. This dosage form has good fluidity, is convenient to use, has no dust flying, and is safe and reliable.

[0045] The present invention also provides the use of the bactericidal mixture containing triazole compounds as described above in controlling plant diseases, and the bactericidal mixture is used for one or more of the plant, the leaves of the plant, the seeds of the plant, and the area near the plant.

[0046] The present invention also provides an application of a bactericidal mixture containing a triazole compound in controlling plant diseases, where the plant diseases are plant diseases caused by fungi or bacteria;

[0047] Further, the plant diseases are plant diseases caused by fungi;

[0048] Further, the plant diseases caused by fungi are Fusarium head blight and powdery mildew of wheat.

[0049] By optimizing the content of the active ingredients in the bactericidal mixture, the present invention achieves a better balance between toxicity and residue, enhances the efficacy of the drug, reduces the dosage of the drug, and lowers the cost.

[0050] Compared with the prior art, the beneficial effects of the technical solution of the present invention are as follows:

[0051] (1) It increases the bactericidal activity, expands the application range, and has an obvious yield-increasing effect;

[0052] (2) It is safe and environmentally friendly, can significantly reduce the content of DO in grains, has broad development prospects, reduces the usage amount of pesticides, and lowers the agricultural cost;

[0053] (3) It is ultra-efficient and has a long residual period, can delay the generation of resistance of harmful bacteria, and extend the persistence of the drug. Specific embodiments

[0054] To make the technical solution, purpose, and advantages of the present invention clearer, the present invention is illustrated by the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0055] Test agents: 95% Aminopyrifen technical; 97% fluxapyroxad technical; 95% prothioconazole technical; all of the above technicals are provided by the Research Center of Hailir Pesticides & Chemicals Group Co., Ltd.

[0056] Indoor bioassay of Fusarium head blight

[0057] Referring to the Pesticide Indoor Bioassay Test Guidelines - Fungicides - Part 2: Inhibiting the Mycelial Growth of Pathogenic Fungi - Petri Dish Method NT / T 1156.2 - 2006 of the Agricultural Industry Standard of the People's Republic of China

[0058] Test crops and strains

[0059] Test pathogen: Fusarium graminearum, collected from diseased wheat ears in Henan region for isolation. Look for pink mold layers at the base of the glumes of the wheat ears, pick the pink mold layers with toothpicks and transfer them to the culture medium, and culture them in a constant temperature incubator for standby.

[0060] Reagent preparation: The test technical material is first dissolved in acetone and then diluted with a 0.1% Tween 80 aqueous solution. Single-agent mother liquors are prepared separately, and 5 series of mass concentrations are set according to the purpose of mixing and the activity of the reagent, with the final content of the organic solvent not exceeding 2%.

[0061] After melting the PDA medium in a microwave oven, cool it to about 50 °C. According to the principle from low concentration to high concentration, take 1 mL of the prepared test liquid to be measured and 9 mL of the PDA medium and add them to a petri dish with a diameter of 9 cm, mix well, and make a drug-containing plate with the corresponding concentration.

[0062] For the cultured pathogenic bacteria, under sterile conditions, use a punch with a diameter of 5 mm to punch the activated pathogenic fungi into fungus cakes. After the drug-containing medium solidifies, place the fungus cakes at the center of the medium. Finally, seal the petri dish with a sealing film and then place it in an incubator at 27 °C for cultivation. At the same time, set up a blank solution without the reagent as a blank control, and repeat each treatment three times.

[0063] After culturing for 72 h, use a caliper to measure the colony diameter, with the unit of centimeter (cm). Measure the diameter of each colony vertically once using the cross method and take the average value.

[0064] Calculate the mycelial growth inhibition rate according to the following formula, with the unit of percentage (%), and retain two decimal places for the calculation result.

[0065] D = D 1 - D 2 ············(1)

[0066] D —— Colony growth diameter;

[0067] D 1 —— Colony diameter;

[0068] D 2 —— Fungus cake diameter.

[0069] I = (D 0 - D t ) / D 0 * 100············(2)

[0070] In the formula:

[0071] I —— Mycelial growth inhibition rate;

[0072] D 0 —— Blank control colony growth diameter;

[0073] D t —— Drug-treated colony growth diameter.

[0074] Statistical analysis: Regression analysis was performed based on the logarithmic values ​​of each agent concentration and the corresponding probability value of mycelium growth inhibition rate to calculate the EC of each agent. 50 The correlation coefficient R is used to evaluate the activity of the test agent on the biological test material.

[0075] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). The co-toxicity coefficient of the compound CTC ≥ 120 indicates a synergistic effect; CTC ≤ 80 indicates an antagonistic effect; 80 < CTC < 120 indicates an additive effect. The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (3), formula (4), and formula (5):

[0076]

[0077] Where:

[0078] ATI——Actual Toxicity Index of Mixture;

[0079] S——EC of standard fungicide 50 , the unit is milligrams per liter (mg / L);

[0080] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0081] TTI=TI A *P A +TI B *P B ·······(4)

[0082] Where:

[0083] TTI – Theoretical Toxicity Index of Mixtures;

[0084] TI A ——A: toxicity index of the agent;

[0085] P A ——The percentage of agent A in the mixture, in percentage (%);

[0086] TI B ——Toxicity index of agent B;

[0087] P B ——The percentage of agent B in the mixture, in percentage (%).

[0088]

[0089] Where:

[0090] CTC – Co-toxicity coefficient;

[0091] ATI——Actual Toxicity Index of Mixture;

[0092] TTI - Theoretical Toxicity Index of the mixture

[0093] Example 1

[0094] Indoor Bioassay of Aminopyrifen and Fluopyram against Fusarium Head Blight of Wheat

[0095] Table 1 results show that the EC of 95% Aminopyrifen technical for controlling Fusarium head blight of wheat 50 was 4.124 mg / L respectively, and the EC of 97% Fluopyram technical for controlling Fusarium head blight of wheat 50 was 5.311 mg / L. The bactericidal mixtures of Aminopyrifen and Fluopyram at ratios of 1:50 to 50:1 all showed good control effects. The co-toxicity coefficients of the bactericidal mixtures of Aminopyrifen and Fluopyram at ratios of 1:30 to 15:1 were > 120, all showing synergistic effects. Among them, the co-toxicity coefficient value of Aminopyrifen:Fluopyram = 1:5 was the largest, being 149.275 > 120, and the EC 50 was 3.395 mg / L, showing a synergistic effect.

[0096] Table 1 Determination results of the synergistic effects of different ratios of Aminopyrifen and Fluopyram on Fusarium head blight of wheat

[0097]

[0098]

[0099] Note: The above data were analyzed using IBM SPSS Statistics 2.0 statistical analysis software, and the values were retained to 3 decimal places. The same applies to Table 2 below.

[0100] Example 2

[0101] Indoor Bioassay of Aminopyrifen and Prothioconazole against Fusarium Head Blight of Wheat

[0102] Table 2 results show that the EC of 95% Prothioconazole technical for controlling Fusarium head blight of wheat 50 was 5.917 mg / L respectively. The bactericidal mixtures of Aminopyrifen and Prothioconazole at ratios of 1:50 to 50:1 all showed good control effects. The co-toxicity coefficients of 1:30 to 15:1 were > 120, all showing synergistic effects. Among them, the co-toxicity coefficient value of Aminopyrifen:Prothioconazole = 1:2 was the largest, being 176.023 > 120, and the EC 50 was 2.936 mg / L, showing a synergistic effect.

[0103] Table 2 Results of the synergistic effect of different ratios of Aminopyrifen and Prothioconazole on wheat fusarium head blight

[0104] Test agents Regression equation <![CDATA[r 2 > <![CDATA[EC 50 > Coefficient of co-toxicity Effect Biopesticide (Y＝) / (mg / L) (ATI) (TTI) (CTC) / Aminopyrifen 1.797x-1.106 0.994 4.124(3.172-5.253) 100.000 / / / Prothioconazole 1.527x-1.179 0.993 5.917(4.420-7.095) 69.697 / / / Aminopyrifen: Prothioconazole = 1:50 1.484x-1.078 0.99 5.322(3.901-7.120) 77.490 70.292 110.240 Additive Aminopyrifen: Prothioconazole = 1:30 1.494x-1.060 0.994 5.118(3.742-6.822) 80.578 70.675 114.013 Additive Aminopyrifen: Prothioconazole = 1:20 1.793x-1.195 0.966 4.640(3.641-6.160) 88.879 71.140 124.935 Synergistic Aminopyrifen: Prothioconazole = 1:8 1.641x-1.070 0.996 4.490(3.455-6.007) 91.849 73.064 125.709 Synergistic Aminopyrifen: Prothioconazole = 1:4 1.589x-0.780 0.997 3.095(2.345-4.103) 133.247 75.758 175.885 Synergistic Aminopyrifen: Prothioconazole = 1:2 1.560x-0.730 0.994 2.936(2.205-3.897) 140.463 79.798 176.023 Synergistic Aminopyrifen: Prothioconazole = 2:1 1.610x-0.820 0.997 3.232(2.462-4.282) 127.639 89.899 141.980 Synergistic Aminopyrifen: Prothioconazole = 4:1 1.578x-0.853 0.996 3.474(2.643-4.665) 118.710 93.939 126.369 Synergistic Aminopyrifen: Prothioconazole = 20:1 1.773x-1.160 0.997 4.509(3.531-5.984) 91.462 98.557 92.801 Additive Aminopyrifen: Prothioconazole = 50:1 1.682x-1.202 0.996 5.184(4.014-6.917) 79.552 99.406 80.028 Additive

[0105] Preparation Example 1: 24% Aminopyrifen·Cyclofoconazole wettable powder (4:20)

[0106] The active ingredient A Aminopyrifen is 4%, the active ingredient B cypermethrin is 20%, the wetting agent alkylnaphthalene sulfonate is 6%, the dispersant alkylnaphthalene formaldehyde condensate sodium sulfonate is 6%, and kaolin is supplemented to 100%. The active ingredients and the auxiliary agents are mixed evenly, put into a mechanical pulverizer for primary pulverization, then pulverized by a jet mill, and then mixed evenly to obtain 24% Aminopyrifen cypermethrin wettable powder.

[0107] Preparation Example 2: 30% Aminopyrifen·Cyclofoconazole dispersible oil suspension (5:25)

[0108] The active ingredient A Aminopyrifen is 5%, the active ingredient B clofoconazole is 25%, the emulsifier calcium dodecylbenzene sulfonate is 2%, styrylphenol polyoxyethylene ether is 1%, the dispersant alkylphenol polyoxyethylene ether is 5%, the thickener gum arabic is 2%, and the soybean oil is supplemented to 100%. The active ingredients, surfactants and other functional additives are placed in a reactor in sequence, oil is added and mixed evenly, and then subjected to high-speed shearing, wet sand grinding, and finally homogenized filtration to obtain a 30% Aminopyrifen clofoconazole dispersible oil suspension.

[0109] Preparation Example 3: 35% Aminopyrifen·Cyclofodazole Suspension Concentrate (5:30)

[0110] Active ingredient A Aminopyrifen is 5%, active ingredient B clofoconazole is 30%, wetting agent lignin sulfonate is 4%, dispersant alkyl naphthalene formaldehyde condensate sodium sulfonate is 4%, antifreeze agent propylene glycol is 3%, thickener magnesium aluminum silicate is 0.1%, and deionized water is added to 100%. The active ingredient, wetting agent, dispersant, antifreeze agent, and water are added to a stirring tank, stirred thoroughly, and then crushed with a sand mill to D 90 (90% of the particle size) <10 μm. After grinding, the mixture is pumped into a high-speed shearing machine, and after adding a thickener, high-speed shearing is performed. After shearing, a 35% Aminopyrifen·Cyclomethasone suspension is obtained.

[0111] Preparation Example 4: 40% Aminopyrifen·Cyclofoconazole dispersible oil suspension (8:32)

[0112] The active ingredient A, Aminopyrifen, is 8%, the active ingredient B, fluxapyroxad, is 32%, the emulsifier calcium dodecylbenzenesulfonate is 2%, the alkylphenol formaldehyde resin polyoxyethylene ether is 1%, the dispersant alkylphenol polyoxyethylene ether is 5%, the thickener gum arabic is 2%, and corn oil is added to make up 100%. The active ingredients, surfactants, and other functional auxiliaries are successively placed in a reaction kettle, mixed evenly with oil, subjected to high-speed shearing and wet sand grinding, and finally homogenized and filtered to obtain 40% Aminopyrifen·fluxapyroxad dispersible oil suspension concentrate.

[0113] Preparation Example 5: 20% Aminopyrifen·prothioconazole suspension concentrate (5:15)

[0114] The active ingredient A, Aminopyrifen, is 5%, the active ingredient B, prothioconazole, is 15%, the wetting agent castor oil polyoxyethylene ether is 5%, the dispersant alkylnaphthalene formaldehyde condensate sulfonate is 5%, the antifreeze propylene glycol is 3%, the thickener organic bentonite is 0.2%, and deionized water is added to make up 100%. The active ingredients, wetting agent, dispersant, antifreeze, and water are put into a stirring kettle, fully stirred, and then crushed with a sand mill until D 90 (The particle size of 90% of the particles) < 10 μm. After the grinding is completed, it is pumped into a high-speed shearing machine, the thickener is added, and then high-speed shearing is carried out. After the shearing is completed, 20% Aminopyrifen·prothioconazole suspension concentrate is prepared.

[0115] Preparation Example 6: 25% Aminopyrifen·prothioconazole suspension concentrate (10:15)

[0116] The active ingredient A, Aminopyrifen, is 10%, the active ingredient B, prothioconazole, is 15%, the wetting agent lignosulfonate is 4%, the dispersant alkylnaphthalene formaldehyde condensate sulfonate is 4%, the antifreeze propylene glycol is 2%, the thickener magnesium aluminum silicate is 0.1%, and deionized water is added to make up 100%. The active ingredients, wetting agent, dispersant, antifreeze, and water are put into a stirring kettle, fully stirred, and then crushed with a sand mill until D 90 (The particle size of 90% of the particles) < 10 μm. After the grinding is completed, it is pumped into a high-speed shearing machine, the thickener is added, and then high-speed shearing is carried out. After the shearing is completed, 25% Aminopyrifen·prothioconazole suspension concentrate is prepared. Preparation Example 7: 30% Aminopyrifen·prothioconazole dispersible oil suspension concentrate (10:20)

[0117] The active ingredient A, Aminopyrifen, is 10%, the active ingredient B, prothioconazole, is 20%, the emulsifier calcium dodecylbenzenesulfonate is 2%, the alkylphenol formaldehyde resin polyoxyethylene ether is 2%, the dispersant alkylphenol polyoxyethylene ether is 5%, the thickener xanthan gum is 2%, and soybean oil is added up to 100%. The active ingredients, surfactants and other functional auxiliaries are sequentially placed in a reaction kettle, mixed evenly with oil, subjected to high-speed shearing and wet grinding, and finally homogenized and filtered to obtain 30% Aminopyrifen·prothioconazole dispersible oil suspension.

[0118] Preparation Example 8: 35% Aminopyrifen·prothioconazole wettable powder (15:20)

[0119] The active ingredient A, Aminopyrifen, is 15%, the active ingredient B, prothioconazole, is 20%, the wetting agent sodium dodecyl sulfate is 6%, the dispersant sodium naphthalene formaldehyde condensate sulfonate is 6%, and diatomaceous earth is added up to 100%. The active ingredients and various auxiliaries are mixed evenly, put into a mechanical pulverizer for preliminary pulverization, then pulverized by a jet mill, and mixed evenly again to obtain 35% Aminopyrifen·prothioconazole wettable powder.

[0120] Field efficacy test of wheat scab

[0121] Test site: Conducted in the wheat planting areas where wheat scab often occurs in Hebei Province all year round

[0122] Test time: Early May 2019, the initial ear stage of wheat

[0123] Test treatments: Single agents and clear water control were set, with a 10-day interval between two applications. The spraying times were the initial ear stage and the flowering stage of wheat respectively. The plot test area for each treatment was 667 m 2 , without repetition. A conventional electric sprayer was used for spraying.

[0124] Investigation method: Five-point sampling was used for fixed-point investigation. For each treatment, 1 m was investigated at each point 2 , and the total ear number and diseased ear number were investigated 10 days before harvest. The disease severity was divided according to the disease situation of wheat ears, with a total of 5 levels:

[0125] Level 0, disease-free;

[0126] Level 1, the number of diseased wheat ears accounts for less than 1 / 4 of all wheat ears;

[0127] Level 2, the number of diseased wheat ears accounts for 1 / 4 - 1 / 2 of all wheat ears;

[0128] Level 3, the number of diseased wheat ears accounts for 1 / 2 - 3 / 4 of all wheat ears;

[0129] Level 4, the number of diseased wheat ears accounts for more than 3 / 4 of all wheat ears.

[0130] The disease index was calculated according to the formula: Disease index = {∑(number of diseased plants at each level × value of that disease level) / (total number of plants surveyed × maximum disease level)} × 100.

[0131] The control effect (%) was calculated according to the formula: Control effect = (control disease index - treatment disease index) / control disease index × 100.

[0132] Calculated according to unit area for 1 m 2 Average number of ears per plant, diseased ear rate.

[0133] Samples were taken at the wheat harvest stage. After drying for 3 days, the 667 m² of each plot was measured 2 Actual measured yield.

[0134] The data was processed using Microsoft Excel 2003, and data statistical analysis and significance test of differences (Duncan method) were performed using IBM SPSS Statistics 2.0.

[0135] Example 3

[0136] Field efficacy test results of aminopyrifen and fluxapyroxad against Fusarium head blight of wheat

[0137] Details of the application rates are shown in Table 3.

[0138] Table 3 Comparison of application rates in field trials

[0139] Treatment Agent <![CDATA[Dosage g a.i / hm 2 > A1 24% Aminopyrifen·Tebuconazole wettable powder (4:20) 100 A2 30% Aminopyrifen·Tebuconazole dispersible oil suspension (5:25) 100 A3 35% Aminopyrifen·Tebuconazole suspension (5:30) 100 A4 40% Aminopyrifen·Tebuconazole dispersible oil suspension (8:32) 100 A5 30% Aminopyrifen suspension 150 A6 400 g / L Tebuconazole suspension 150 A7 Water control /

[0140] Table 4 results showed that through the field efficacy plot trials, the survey results 10 days before harvest showed that the 30% aminopyrifen·fluxapyroxad dispersible oil suspension concentrate (5:25) had the best control effect against Fusarium head blight of wheat. The diseased ear rate in the treated plot was 6.25%, and the control effect was 82.83%. The control effects of the 35% aminopyrifen·fluxapyroxad suspension concentrate (5:30) and 24% aminopyrifen·fluxapyroxad suspension concentrate (4:20) against Fusarium head blight of wheat were 80.64% and 80.48% respectively. At the 0.01 and 0.05 levels, there was no significant difference in the control effects of the two agents against Fusarium head blight of wheat; the control effect of the 40% aminopyrifen·fluxapyroxad water dispersible granule (8:32) was 79.56%. Compared with the control single agents 30% aminopyrifen suspension and 400 g / L fluxapyroxad suspension, it also showed a good control effect. The control effects of the 30% aminopyrifen suspension and 400 g / L fluxapyroxad suspension were 73.31% and 66.58% respectively. Treatment A 1 ~A 4There were significant differences between any bactericidal mixture and two control single agents at the 0.01 level and 0.05 level.

[0141] Table 4 Control of Gibberella zeae in wheat by different combinations and single agents of Aminopyrifen and cyflufenamid

[0142]

[0143] Note: The data of each treatment above were calculated per unit area as 1 m 2 Average number of ears per plant and diseased ear rate; the control effect % in the above table is the average of each repetition; lowercase letters represent significant differences at the 5% level, and uppercase letters represent significant differences at the 1% level.

[0144] Table 5 Effect of different agents on controlling Gibberella zeae in wheat on wheat yield

[0145] Treatment <![CDATA[Yield / kg / 667m 2 > Difference Safety <![CDATA[A 1 : 24% Aminopyrifen·Tebuconazole wettable powder (4:20)]]> 438.28 b Normal <![CDATA[A 2 : 30% Aminopyrifen·fluxapyroxad dispersible oil suspension concentrate (5:25)]]> 445.17 a Normal <![CDATA[A 3 : 35% Aminopyrifen·Tebuconazole Suspension Concentrate (5:30)]]> 426.14 c Normal <![CDATA[A 4 : 40% Aminopyrifen·Tebuconazole Dispersible Oil Suspension (8:32)]]> 433.44 c Normal <![CDATA[A 5 : 30% Aminopyrifen Suspension Concentrate]]> 431.29 c Normal <![CDATA[A 6 : 400 g / L Pyrifluoxastrobin Suspension Concentrate]]> 429.57 c Normal <![CDATA[A 7 : Clear water control]]> 381.51 d Normal

[0146] The yield in the above table is the average of each repetition; lowercase letters represent significant differences at the 5% level.

[0147] The results of the field efficacy plot test showed that in the process of controlling Gibberella zeae in wheat by the combination of Aminopyrifen and cyflufenamid, not only the diseased ear rate and disease index were reduced, with significant control effects, but also there was not much impact on the yield. Among them, 40% Aminopyrifen·cyflufenamid dispersible oil suspension (8:32) also showed an obvious yield increase effect. In addition, according to the observation after application, the wheat growth in each treatment area A 1 ~A 7 was normal, and there was no phytotoxicity phenomenon, indicating that each agent was safe for wheat growth at the dosage used in this experiment.

[0148] Example 4

[0149] Field efficacy test of Aminopyrifen and prothioconazole against Gibberella zeae in wheat

[0150] Details of the application rates are shown in Table 6.

[0151] Table 6 Comparison table of application rates for field trials

[0152] Treatment Agent Dosage g a.i / hm2 <![CDATA[B 1 > 20% Aminopyrifen·Prothioconazole suspension (5:15) 100 <![CDATA[B 2 > 25% Aminopyrifen·Prothioconazole suspension (10:15) 100 <![CDATA[B 3 > 30% Aminopyrifen·Prothioconazole dispersible oil suspension (10:20) 100 <![CDATA[B 4 > 35% Aminopyrifen·Prothioconazole wettable powder (15:20) 100 <![CDATA[B 5 > 30% Aminopyrifen suspension 150 <![CDATA[B 6 > 30% Prothioconazole dispersible oil suspension 180 <![CDATA[B 7 > <![CDATA[B 9 : Clear water control]]> /

[0153] The results in Table 7 showed that through the field efficacy plot trials, the survey results 10 days before harvest showed that the diseased ear rates in the plots treated with 35% Aminopyrifen·prothioconazole wettable powder (15:20) and 30% Aminopyrifen·prothioconazole dispersible oil suspension (10:20) were 6.35% and 6.23% respectively, and the control efficacies were 86.21% and 86.16% respectively. The control efficacies of 25% Aminopyrifen·prothioconazole suspension (10:15) and 20% Aminopyrifen·prothioconazole suspension (5:15) against Fusarium head blight of wheat were 84.16% and 83.35% respectively. There were significant differences between the two treatments at the 0.01 level and 0.05 level, and the control efficacy of each compounding was more than 80%. The control efficacies of 30% Aminopyrifen suspension and 30% prothioconazole dispersible oil suspension were 75.21% and 66.72% respectively. Treatment B 1 ~B 4 There were significant differences between any bactericidal mixture and the two control single agents at the 0.01 level and 0.05 level.

[0154] Table 7 Control of Fusarium head blight of wheat by different compoundings and single agents of Aminopyrifen and prothioconazole

[0155]

[0156]

[0157] Note: The data of each above treatment were calculated according to the average ear number and diseased ear rate per unit area of 1 m 2 The control efficacy % in the above table was the average value of each repetition; lowercase letters represented significant differences at the 5% level, and uppercase letters represented significant differences at the 1% level.

[0158] Table 8 Effects of different agents on controlling Fusarium head blight of wheat on wheat yield

[0159] Treatment <![CDATA[Yield / kg / 667m 2 > Difference Safety <![CDATA[B 2 : 25% Aminopyrifen · Prothioconazole Suspension Concentrate (10:15)]]> 433.75 a Normal <![CDATA[B 3 : 30% Aminopyrifen · Prothioconazole Dispersible Oil Suspension (10:20)]]> 403.78 c Normal <![CDATA[B 4 : 35% Aminopyrifen · Prothioconazole Wettable Powder (15:20)]]> 433.89 a Normal <![CDATA[B 5 : 30% Aminopyrifen Suspension Concentrate]]> 436.51 a Normal <![CDATA[B 6 : 30% Prothioconazole Dispersible Oil Suspension]]> 429.48 b Normal <![CDATA[B 7 : Clear water control]]> 386.55 d Normal

[0160] The yield in the above table was the average value of each repetition; lowercase letters represented significant differences at the 5% level.

[0161] The results of the field efficacy plot trials showed that in the process of controlling Fusarium head blight of wheat by compounding Aminopyrifen and prothioconazole, not only the diseased ear rate and disease index were reduced, but also there was a significant control effect, and there was not much impact on the yield. Among them, 35% Aminopyrifen·prothioconazole wettable powder (15:20) and 30% Aminopyrifen·prothioconazole dispersible oil suspension (10:20) also showed obvious yield-increasing effects. In addition, according to the observation after application, in each treatment area A -1 ~A7 The wheat growth was normal, and no phytotoxicity occurred, indicating that the wheat was safe for growth under the dosages of each agent in this experiment.

[0162] Example 5

[0163] Indoor bioassay of wheat powdery mildew

[0164] Test materials: The tested wheat variety Yumai 34 is a highly susceptible variety to wheat powdery mildew.

[0165] Test pathogen: Wheat powdery mildew (Blumeria graminis f.sp.tritici)

[0166] After wheat seeds were germinated, the wheat was sown quantitatively in a 30-cm-diameter planting pot. When the seedlings grew to the one-leaf and one-heart stage, they were placed in a 25°C light incubator and cultured with an alternating light-dark ratio of 16:8 h. The wheat growth was uniform and consistent, and it was reserved for use.

[0167] The fresh wheat powdery mildew spore suspension was sprayed onto the reserved wheat seedlings, with 2 pots for each treatment and 15 plants in each pot. After inoculation for 72 h, the agent treatment was carried out. The disease index of each treatment was investigated before the application of the agent, and the disease index was investigated once every 5 days after the application of the agent until all the controls were diseased, and the control effect was calculated.

[0168] Table 9 Treatment concentrations of tested agents for controlling wheat powdery mildew

[0169]

[0170]

[0171] Table 10 The results of the indoor pot experiment showed that 5 days after the application of the agent, the average control effects of the treatments with 30% Aminopyrifen·prothioconazole dispersible oil suspension (10:20) and 30% Aminopyrifen·trifloxystrobin dispersible oil suspension (5:25) were the best, being 75.16% and 74.44% respectively. The control effects of the treatments with 24% Aminopyrifen·trifloxystrobin wettable powder (4:20), 35% Aminopyrifen·trifloxystrobin suspension (5:30), 25% Aminopyrifen·prothioconazole suspension (10:15) and 35% Aminopyrifen·prothioconazole wettable powder (15:20) were 72.58%, 72.26%, 71.93% and 71.99% respectively. There was no significant difference among the treatments, and they were significantly better than the control single-agent treatments with 30% Aminopyrifen suspension, 400 g / L trifloxystrobin suspension and 30% prothioconazole dispersible oil suspension.

[0172] 10 days after the application, the control effects of 30% Aminopyrifen suspension, 400 g / L clofoconazole suspension and 30% prothioconazole dispersible oil suspension were 64.93%, 62.63% and 63.55% respectively. The control effect increased with the increase of time. 30% Aminopyrifen·Prothioconazole dispersible oil suspension (10:20) had the best control effect, with a control effect of 76.25%, which was significantly better than the control effects of other concentrations. 30% Aminopyrifen·clofoconazole dispersible oil suspension (5:25) had a control effect of 74.62%, which also showed a good control effect.

[0173] Table 10 Treatment concentrations of the tested agents for controlling wheat powdery mildew

[0174]

[0175]

[0176] The protective effects in the above table are the average values ​​of each replicate; lowercase letters represent significant differences at the 5% level.

[0177] Example 6

[0178] Field test on the efficacy of pesticides against wheat powdery mildew

[0179] Test variety: Yumai No. 34; control target: wheat powdery mildew.

[0180] The randomized block arrangement was adopted, and the area of ​​the plot was 25m 2 , repeated 4 times. Sampling was carried out at 5 points in each plot, 10 plants were surveyed at each point, and the flag leaf and the first leaf under the flag leaf were surveyed for each plant.

[0181] The test site was at the planting area in Tongbai County, Nanyang City, Henan Province, and the application time was mid-May 2019.

[0182] Five fixed sampling points were taken on the diagonal of each plot, and each point was 0.25m 2 For wheat plants, investigate 1 to 5 leaves at the base during the jointing stage, and investigate the flag leaf and the first leaf under the flag leaf of each plant after heading. Before applying the pesticide, investigate the occurrence of wheat powdery mildew on fixed plants and record the disease index. Investigate once at 7 and 21 days after applying the pesticide, and calculate the prevention effect.

[0183] The classification survey and classification standards were based on the field efficacy test guidelines GB / T 17980.22-2000, and the test data were analyzed using Duncan's new multiple range method.

[0184] A 9-level grading system is used:

[0185] Grading method of powdery mildew (based on leaves):

[0186] Grade 0: No lesions;

[0187] Grade 1: Lesion area less than 5% of the total leaf area;

[0188] Grade 3: Lesion area 6% - 10% of the total leaf area;

[0189] Grade 5: Lesion area 11% - 20% of the total leaf area;

[0190] Grade 7: Lesion area 21% - 50% of the total leaf area;

[0191] Grade 9: Lesion area more than 50% of the total leaf area.

[0192] The drug efficacy is calculated according to formulas (1) and (2):

[0193] (1) Disease index = ∑{(number of diseased leaves at each level * relative level value) / (total number of leaves surveyed * 9)} * 100

[0194] (2) Control effect (%) = {1 - (disease index before treatment in blank control area * disease index after treatment in treatment area) / (disease index after treatment in blank control area * disease index before treatment in treatment area)} * 100

[0195] Or control effect (without base number before application) % = (disease index in blank control area - disease index in treatment area) / disease index in blank control area * 100

[0196] Data was processed using Microsoft Excel 2003, and data statistical analysis and significance test of differences (Duncan method) were performed using IBM SPSS Statistics 2.0.

[0197] The results of the field medicament test for controlling wheat powdery mildew showed that before application, the disease index in each treatment plot was between 2.16 and 3.45. 14 days after application, the disease indices of 30% Aminopyrifen·Prothioconazole dispersible oil suspension (10:20) and 30% Aminopyrifen·Tebuconazole dispersible oil suspension (5:25) were 6.58 and 5.68 respectively, and the control effects were 85.23% and 86.03% respectively. The control effects of the four treatments of 24% Aminopyrifen·Tebuconazole wettable powder (4:20), 35% Aminopyrifen·Tebuconazole suspension (5:30), 25% Aminopyrifen·Prothioconazole suspension (10:15), and 35% Aminopyrifen·Prothioconazole wettable powder (15:20) were 82.23%, 81.61%, 80.04%, and 80.12% respectively, and the control effects were relatively good, D 1 ~D 8The control effects of each treatment were significantly better than those of the control single agent.

[0198] 21 days after application, the control effects of 30% Aminopyrifen·Cyclofluconazole dispersible oil suspension (5:25) and 30% Aminopyrifen·Prothioconazole dispersible oil suspension (10:20) were improved to 86.51% and 86.24% respectively, showing good persistence. The combination of Aminopyrifen and Prothioconazole was better than 30% Prothioconazole dispersible oil suspension, and the control effect of each combination was higher than 74.65%. The control effects of 24% Aminopyrifen·Cyclofluconazole wettable powder (4:20) and 35% Aminopyrifen·Cyclofluconazole suspension (5:30) were 76.18% and 76.04% respectively, and there was no significant difference in the control effects of the two treatments.

[0199] Table 11 Treatment concentrations of tested agents for controlling wheat powdery mildew

[0200]

[0201] The protective effects in the above table are the average values ​​of each replicate; lowercase letters represent significant differences at the 5% level.

[0202] The results of the above indoor and field tests show that the combination of Aminopyrifen with clofoconazole and prothioconazole has a good control effect on wheat powdery mildew. The control effect of clofoconazole and prothioconazole alone is significantly lower than that of the combined agents. In the test process, each agent is safe to wheat at the test concentration and has no phytotoxicity. Therefore, the combination of Aminopyrifen with clofoconazole and prothioconazole can alleviate the increasing trend of powdery mildew resistance year by year, improve the control effect of wheat powdery mildew and extend the service life of the agent.

[0203] Through indoor toxicity determination and field tests, the mixture of Aminopyrifen and clofoconazole and prothioconazole of the present invention showed good control effect on fungal diseases of crops (wheat fusarium head blight and wheat powdery mildew). The fungicidal mixture or its preparation obtained by compounding of the present invention has significant control effect, and has the characteristics of high efficiency, broad spectrum, low residue, long lasting effect, strong systemicity, etc.; and no phytotoxicity of the compounded agent to crops was found in the test, indicating that when the fungicidal synergistic effect of the obtained fungicidal mixture or preparation is improved, the production cost and use cost can be reduced, and it is safe for crops.

[0204] Although the present invention has been described in detail above with general descriptions and specific embodiments, modifications or improvements can be made to it on the basis of the present invention, which are obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection required by the present invention.

Claims

1. A bactericidal mixture containing triazole compounds, Characterized in that: Containing active ingredient A and active ingredient B, where active ingredient A is a compound represented by formula (I), active ingredient B is the triazole fungicide prothioconazole, and the mass ratio of the compound of formula (I) to prothioconazole is 4:1 to 1:

20. (I).

2. The bactericidal mixture according to claim 1, Characterized in that, The mass ratio of the compound of formula (I) to prothioconazole is 4:1 to 1:

8.

3. The bactericidal mixture according to claim 1, Characterized in that, Based on the total weight of the bactericidal mixture being 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the bactericidal mixture is 1 to 95 wt%.

4. The bactericidal mixture according to claim 3, Characterized in that, Based on the total weight of the bactericidal mixture being 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the bactericidal mixture is 5 to 80 wt%.

5. The bactericidal mixture according to claim 4, Characterized in that, Based on the total weight of the bactericidal mixture being 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the bactericidal mixture is 10 to 60 wt%.

6. The bactericidal mixture according to claim 1, Characterized in that, The bactericidal mixture further comprises adjuvants selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

7. The bactericidal mixture according to claim 6, Characterized in that, The dosage form of the bactericidal mixture can be any one of emulsifiable concentrates, emulsifiable water concentrates, microemulsions, suspensions, oil-dispersible suspensions, wettable powders or water-dispersible granules.

8. The bactericidal mixture according to claim 7, Characterized in that, The dosage form is any one of wettable powders, suspensions, oil-dispersible suspensions.

9. The application of the bactericidal mixture according to any one of claims 1-8 in the prevention and control of plant diseases, Characterized in that, The bactericidal mixture is used for one or more of the plant, the leaves of the plant, the seeds of the plant and the area near the plant, and the plant diseases are wheat head blight and wheat powdery mildew.

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