An interference agent for spodoptera exigua and mythimna separata and application thereof

Abstract

The application discloses a kind of Spodoptera frugiperda and labra moth interference agent, comprising: cis-9-tetradecene alcohol and auxiliary agent, and the mass ratio of cis-9-tetradecene alcohol and auxiliary agent is (0.005-1):1, different daily release amount of the interference agent can have different interference effects on Spodoptera frugiperda and labra moth, reduce the number of Spodoptera frugiperda or labra moth attracted, accurately capture one kind of pest;And can effectively interfere with the mating of Spodoptera frugiperda male and female, labra moth male and female, reduce mating rate, in turn reduce the number of pests.

Description

Technical Field

[0001] This invention relates to the fields of plant protection and insect control technology, and more specifically to an interfering agent for fall armyworm and armyworm and its application. Background Technology

[0002] The fall armyworm (Spodopterafrugiperda), native to tropical and subtropical regions of the Americas, is a major agricultural pest under global early warning by the Food and Agriculture Organization of the United Nations (FAO). It possesses strong migratory capabilities and, as a major invasive alien species, began spreading to China in January 2019, posing a significant threat to domestic food security. The armyworm (Leucania loryi), distributed in Guangdong, Fujian, Sichuan, Jiangxi, Hunan, Hubei, Zhejiang, Jiangsu, Shandong, and Henan provinces of my country, has larvae with a diverse diet, feeding on various plants, particularly grasses, primarily damaging wheat, rice, and forage crops. Currently, chemical control is the primary method used in China to manage these two pests, but this approach is limited and its effectiveness is limited. Green pest control, a technology vigorously developed and promoted in my country, significantly reduces the use of chemical pesticides and improves their efficacy.

[0003] Applying insect pheromones to control pests is an important green control technology, boasting advantages such as high efficiency, low toxicity, no pollution, and no harm to natural enemies, bringing significant ecological, social, and economic benefits. Specific pests can be captured using specific sex pheromones combined with traps, enabling precise monitoring and guidance for pest control. Furthermore, large-scale trapping of specific pests can also be used for control. Additionally, releasing high concentrations of sex pheromones can disrupt the mating of female and male adult target pests, reducing the population size of the next generation and achieving control.

[0004] Because the fall armyworm and the armyworm have similar pheromone components, trapping the fall armyworm will also trap the armyworm, causing difficulties in accurate monitoring. In addition, domestic and foreign research institutions have developed insect pheromone interference products that can target both the fall armyworm and the armyworm, but these products have more components and are more expensive.

[0005] Therefore, how to provide a single-species attractant product, as well as a new product for interfering with the pheromones of fall armyworm and armyworm that is simple in composition, cost-effective, and highly effective, is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0006] In view of this, the present invention provides an interfering agent for fall armyworm and armyworm with cis-9-tetradecenol as the main component.

[0007] An interfering agent for fall armyworm and armyworm, comprising: cis-9-tetradecenol and an adjuvant, wherein the mass ratio of cis-9-tetradecenol to the adjuvant is (0.005-1):1.

[0008] The technical effects achieved by the above solutions are as follows: cis-9-tetradecenol, as an insect pheromone, can effectively interfere with the attraction of sex pheromones, thereby interfering with the attraction of sex pheromones to male adult *Armoria serratifolia* or *Fall Armyworm*, thus achieving the effect of precise attraction of a pest; or it can interfere with the mating of male and female *Armoria serratifolia* or *Fall Armyworm*, thus achieving the effect of interfering with the mating of pests.

[0009] Moreover, the interfering agent of the present invention has only cis-9-tetradecenol as the main component. The composition is simple, inexpensive and readily available. It can be released into the field through a variety of slow-release technologies, which effectively reduces the production difficulty and cost of interfering agents for fall armyworm and armyworm.

[0010] Meanwhile, this invention controls the daily release of effective components through adjuvants and sustained-release technology to achieve different interference effects and duration of effect in the field.

[0011] The mechanism of action of pheromone attractants: By mimicking the sex pheromones released by female insects in nature, they attract male insects to mate, and through the use of a matching trap, they achieve the purpose of luring and capturing.

[0012] The mechanism of interference with mating: By releasing a large amount of artificially synthesized sex pheromones into the air, male moths are kept in a high-concentration artificial hormone environment for a long time. The antennal receptors of male moths become adaptive and lose their response to sex pheromones, making them unable to locate female moths. This leads to a decrease in the probability of mating between males and females, thereby reducing the number of insect populations.

[0013] The effectiveness of this interfering agent has been verified through field trials. Furthermore, in existing technologies, this substance is merely a precursor, or it needs to interact with other substances to form a pheromone attractant; it cannot be used alone. Moreover, when combined with other substances, it is not specifically targeted at this particular pest. Different insects in different habitats exist in nature, therefore, targeting this specific insect is a new discovery.

[0014] As a preferred technical solution of the present invention, the additives include: antioxidants, UV stabilizers and solvents, and the mass ratio of the three is (1-10):(1-10):(1-1000).

[0015] The technical effects achieved by the above solutions are as follows: the antioxidant prevents the oxidation of cis-9-tetradecenol by competitively binding with oxygen; the UV protectant can protect the structure of cis-9-tetradecenol and prevent its structural changes, thereby maintaining the effect of cis-9-tetradecenol; the solvent can dissolve cis-9-tetradecenol, the UV protectant and the antioxidant to form a homogeneous system, which better maintains the structure of cis-9-tetradecenol.

[0016] As a preferred embodiment of the present invention, the antioxidant includes one or more of BHT, BHA and TBHQ.

[0017] The technical effects achieved by the above technical solutions are as follows: BHT is produced by reacting cresol and isobutanol with concentrated sulfuric acid as a catalyst and alumina as a dehydrating agent. The resulting 2,6-di-tert-butyl-p-cresol has excellent antioxidant properties and can still exert good antioxidant effects even at high temperatures of 100℃; BHA is butylated hydroxyanisole, which is non-toxic and has good thermal stability, and can be used as an antioxidant for pheromones; TBHQ, or tert-butylhydroquinone, is a strong antioxidant that can scavenge free radicals.

[0018] As a preferred embodiment of the present invention, the UV stabilizer includes one or both of UV-531 and phenyl salicylate.

[0019] The technical effects achieved by the above technical solutions are as follows: UV-531 is an ultraviolet absorber that can strongly absorb ultraviolet rays with wavelengths of 270-330nm, thereby preventing ultraviolet rays from inducing mutagenesis of the cis-9-tetradecenol structure; phenyl salicylate is also an ultraviolet absorber that absorbs some ultraviolet rays, thereby achieving the effects of anti-oxidation and anti-mutation.

[0020] As a preferred embodiment of the present invention, the solvent includes one or more of n-heptane, n-hexane, and ethanol.

[0021] The technical effect achieved by the above technical solution is that the solvent is used to dissolve the UV stabilizer, antioxidant and cis-9-tetradecenool. The disclosed substances such as UV-531 and BHT are easily soluble in n-heptane, n-hexane and alcohol solvents. Therefore, the homogeneity of the system is guaranteed, and the structure of cis-9-tetradecenool is protected from being destroyed.

[0022] As a preferred technical solution of the present invention, the sustained-release technology includes one or more implementations of sustained-release materials and sustained-release devices.

[0023] The technical effect achieved by the above technical solution is that the slow-release carrier and slow-release device encapsulate the fall armyworm and armyworm interfering agent alone or together with the fall armyworm and armyworm pheromone attractant in a slow-release container. Carriers, sustained release devicesInternally, and slowly released into the environment, it inhibits the attraction of fall armyworm and armyworm pheromones, suppressing the attraction effect or mating interference of one or both pests.

[0024] The application of the above-mentioned fall armyworm and armyworm interferon in the precise monitoring and control of fall armyworm.

[0025] The application of the above-mentioned fall armyworm and armyworm interfering agents in the precise monitoring and control of armyworm.

[0026] The technical effects achieved by the above solutions are as follows: when interfering agents are applied to the monitoring of fall armyworm and armyworm, they inhibit and attract one of the pests, thus achieving precise monitoring; when interfering agents are applied to the control of fall armyworm and armyworm, they effectively interfere with the mating of male and female fall armyworms and armyworms, reducing the mating rate and thus reducing the number of pests. Attached Figure Description

[0027] Figure 1 The attached figure shows the results of interference with fall armyworm in different test areas according to the present invention. Detailed Implementation

[0028] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0029] Example 1

[0030] An interfering agent for fall armyworm and armyworm, comprising: 1 part cis-9-tetradecenol, 1 part antioxidant BHT, 1 part ultraviolet absorber UV-531, and 100 parts n-heptane.

[0031] Example 2

[0032] An interfering agent for fall armyworm and armyworm, comprising: 100 parts of cis-9-tetradecenol, mixed with 5 parts of antioxidant BHT, 10 parts of ultraviolet absorber phenyl salicylate, and 1000 parts of n-hexane.

[0033] Example 3

[0034] An interfering agent for fall armyworm and armyworm, comprising: 5 parts of cis-9-tetradecenol, mixed with 10 parts of antioxidant BHT, 10 parts of ultraviolet absorber phenyl salicylate, and 980 parts of n-hexane.

[0035] Example 4

[0036] An interfering agent for fall armyworm and armyworm, comprising: 3 parts of cis-9-tetradecenol, mixed with 2 parts of antioxidant BHT, 2 parts of ultraviolet absorber phenyl salicylate, and 2 parts of n-hexane;

[0037] Example 5

[0038] An interfering agent for fall armyworm and armyworm, comprising: 100 parts of cis-9-tetradecenol, mixed with 2 parts of antioxidant BHT, 2 parts of ultraviolet absorber phenyl salicylate, and 98 parts of n-hexane.

[0039] Example 6 verifies the inhibitory effect of the interfering agent on the attraction of fall armyworm and armyworm pheromone lure cores.

[0040] In a cornfield in Xiangyun Village, Mangshi City, Yunnan Province, an experiment was conducted to investigate the interfering effect of the interfering agent from Example 1 on the attraction of fall armyworm pheromones to fall armyworms. The experiment took place from May 6th to May 15th, 2020. The interfering agent from Example 1 was packaged in a PE bag and suspended outside a barrel-shaped trap (provided by Quanzhou Lvpusen Biotechnology Co., Ltd.). The barrel-shaped trap contained pheromone lures for both fall armyworms and armyworms. Three replicates were set up to form the interference zone. The insect trapping situation was investigated once a day.

[0041] Meanwhile, a regular trapping zone was set up in the same area, with fall armyworm and armyworm pheromone lures placed only inside the barrel-shaped traps (provided by Quanzhou Lvpusen Biotechnology Co., Ltd.), with 3 replicates; the pest trapping situation in the traps was investigated once a day.

[0042] Field trials were conducted to verify the effects of pheromone traps on fall armyworm (represented by "fall armyworm") and armyworm (represented by "Raouli"). Table 1 shows that in the same area, after the presence of interfering agents, the pheromone lures did not trap armyworms, while in the conventionally pheromone-trapping area, the pheromone lures trapped a large number of armyworms. There was no significant difference in the trapping effect of pheromone lures on fall armyworm in both areas. This experiment demonstrates that interfering agents have an inhibitory effect on the attraction of armyworms by pheromone lures, but no effect on the attraction of fall armyworm.

[0043] Table 1. Inhibition of Fall Armyworm and Fall Armyworm Attraction by Sex Pheromone Lure (SLA) by Interfering Agents

[0044]

[0045] Example 7 Field Interference Mating Test of Interference Agent Polyethylene Tube Slow-Release Carrier

[0046] In a cornfield in Xiangyun Village, Mangshi City, Yunnan Province, a mating interference experiment was conducted on the interfering agent in Example 2 from November 15, 2019 to December 31, 2019. A polyethylene tube with a suitable inner diameter was extruded using an extruder as a carrier. The interfering agent was poured into the extruded polyethylene tube, and a 20cm long slow-release tube was prepared using a sealing and cutting device. This yielded the polyethylene tube slow-release carrier samples for interfering with fall armyworm and armyworm in the field trials.

[0047] A field trial was conducted. Polyethylene tubes containing the interferon were tied to corn plants at a dosage of 40 tubes / mu (10g / mu / day). The experimental area was a contiguous 20-mu cornfield, divided into two experimental zones: Experimental Zone 1 and Experimental Zone 2; the control area was 10 mu. Two sets of monitoring traps were placed at the center of each experimental zone, and two sets of control traps were placed in the control zone. All traps were barrel-type (provided by Quanzhou Lvpusen Biotechnology Co., Ltd.). One fall armyworm lure was placed in one barrel-type trap, and one Lawn armyworm lure was placed in the other. Data was collected daily, and the insects in the traps were removed. The interference results are shown in […]. Figure 1 It can be seen that the interfering agent in Example 2 achieved good interference mating effect on both fall armyworm and armyworm in the field.

[0048] The various embodiments in this specification are shown for different situations. Each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0049] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. The application of a Lawn Armyworm disruptor in the precise monitoring of the fall armyworm, characterized in that, The interfering agent is composed of 1 part cis-9-tetradecenol, 1 part antioxidant BHT, 1 part ultraviolet absorber UV-531, and 100 parts n-heptane.

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