Isolated liver perfusion system, connector tube assembly and connection method thereof

Abstract

The present invention relates to an ex vivo liver perfusion system, a joint pipe assembly and a connection method thereof, wherein the joint pipe assembly comprises: an intubation seat and a docking seat. The intubation seat is provided with a first compression end face, and a through hole is located in the middle of the first compression end face. The docking seat is provided with a docking hole, and the docking hole and the through hole are arranged correspondingly. The docking seat is also provided with a second compression end face. The docking hole is located in the middle of the second compression end face, and the second compression end face is compressed and matched with the first compression end face. The docking seat is also provided with a connecting pipe connected to the docking hole, and the connecting pipe is used to be connected to a consumable pipeline. In this way, the inner wall of the portal vein or the proper hepatic artery is not contacted during the perfusion process, thus avoiding damage to the endothelial tissue of the portal vein or the proper hepatic artery, and no suture operation is required, which greatly improves the work efficiency of assembly. In addition, the joint pipe assembly is firmly combined with the artery or portal vein of the ex vivo liver, and there is no risk of falling off during the perfusion process.

Description

Technical Field

[0001] The invention relates to the technical field of perfusion, in particular to an isolated liver perfusion system, a joint pipe assembly and a connection method thereof. Background Art

[0002] Liver transplantation is the only effective means of treating end-stage liver disease. Due to organ shortage, many patients with end-stage liver disease die before they can get a suitable liver transplant. Therefore, the transplantation of marginal livers is of great significance in the field of liver transplantation. The quality of marginal livers is poor, and traditional static cold storage will further aggravate their damage. The ex vivo liver mechanical perfusion (MP) technology has been proposed to effectively improve the quality of marginal livers, thereby increasing the number of transplantable livers.

[0003] Mechanical perfusion of isolated liver is a way to preserve and transport organs. After the liver is harvested, its own blood vessels are connected to the isolated liver mechanical perfusion system. The isolated liver mechanical perfusion system continuously perfuses the liver with perfusion fluid during the liver preservation and transportation stages, while supplying the liver with oxygen and nutrients. The liver reservoir is the core part of the isolated liver mechanical perfusion system. During perfusion, the liver is placed in the liver reservoir and connected to the circulation pipeline through cannulas and connectors on the liver reservoir. The perfusion mode of the liver in the mechanical perfusion system can be divided into two forms according to the pipeline connection method: ① Closed-loop perfusion, that is, the consumable pipeline is connected to the hepatic artery and portal vein of the liver through cannulas, which serve as the inlet for the perfusion fluid to enter the liver; the inferior vena cava of the liver is connected to the consumable pipeline through cannulas, which serves as the outlet for the perfusion fluid to leave the liver, forming a closed circulation pipeline. ② Open-loop perfusion, that is, the consumable pipeline is connected to the hepatic artery and portal vein of the liver through a cannula, which serves as the inlet for the perfusion fluid to enter the liver; the inferior vena cava of the liver is not connected to any cannula, and the perfusion fluid flows directly from the inferior vena cava into the liver reservoir after flowing through the liver, and is then discharged from the outlet of the liver reservoir into the consumable pipeline, forming an open-loop circulation pipeline.

[0004] During perfusion, the liver reservoir acts as an intermediate carrier to connect the liver to the circulation pipeline for circulation perfusion. Therefore, the design of the liver reservoir must not only meet the requirements of comfortable perfusion of the liver, but also meet the requirements of perfect connection to the circulation pipeline for perfusion. The liver arterial cannula or portal vein cannula is the medium that connects the liver to the circulation pipeline. It is usually a long tube. During perfusion, one end of the cannula is connected to the pipeline, and the other end is inserted into the liver portal vein or vein, and then tied with surgical thread for perfusion. Summary of the invention

[0005] Based on this, it is necessary to overcome the defects of the prior art and provide an ex vivo liver perfusion system, a connector tube assembly and a connection method thereof, which can facilitate assembly and connection while avoiding damage to the endothelial tissue of the portal vein or the hepatic artery of the ex vivo liver.

[0006] The technical solution is as follows: a joint pipe assembly, the joint pipe assembly comprising:

[0007] A cannula seat, wherein the cannula seat is provided with a through hole for inserting an artery or portal vein of an isolated liver, and the cannula seat is provided with a first pressing end surface, wherein the through hole is located in the middle of the first pressing end surface;

[0008] The docking seat is provided with a docking hole, the docking hole is arranged corresponding to the position of the through hole, the docking seat is also provided with a second clamping end face, the docking hole is located in the middle of the second clamping end face, the second clamping end face is clamped and matched with the first clamping end face, and the docking seat is also provided with a connecting pipe connected to the docking hole, and the connecting pipe is used to be connected to the consumable pipeline.

[0009] The above-mentioned joint pipe assembly can connect the artery or portal vein of the ex vivo liver to the consumables pipeline, so that the artery or portal vein of the ex vivo liver is connected to the perfusion circulation pipeline. Taking the connection of the artery of the ex vivo liver to the consumables pipeline as an example, the connection process of the artery and the joint pipe assembly is explained. The main trunk of the artery end is retained before perfusion, and the main trunk of the artery end is cut into sheets arranged circumferentially around the artery end. The artery is inserted into the through hole so that the sheet at the artery end is laid flat on the first compression end face, and the first compression end face of the docking seat is compressed and matched with the second compression end face of the cannula seat. At this time, the docking hole is connected to the artery. In addition, the connecting tube is connected to the consumables pipeline, so that the artery of the ex vivo liver is connected to the consumables pipeline. After the ex vivo liver is connected to the perfusion circulation pipeline, the perfusion work can be started. After the perfusion is completed, the docking seat and the cannula seat are separated, and the sheet at the end of the artery is trimmed to complete the perfusion. It can be seen that the cannula is not inserted directly into the artery as in the traditional way. In this way, the inner wall of the artery is not touched during the perfusion process, thus avoiding damage to the endothelial tissue of the artery. At the same time, no suture operation is required, which greatly improves the assembly efficiency. In addition, the connector tube assembly is firmly combined with the artery or portal vein of the isolated liver, and there is no risk of falling off during the perfusion process.

[0010] In one of the embodiments, one side of the intubation seat is movably connected to one side of the docking seat, and the other side of the intubation seat is detachably connected to the other side of the docking seat via a mounting member.

[0011] In one of the embodiments, two U-shaped connecting plates are spaced apart on one side of the tube insertion seat, a connecting rod and a rotating shaft connected to the connecting rod are provided on one side of the docking seat, one end of the rotating shaft is rotatably provided in one of the U-shaped connecting plates and can move along one of the U-shaped connecting plates, the other end of the rotating shaft is rotatably provided in the other U-shaped connecting plate and can move along the other U-shaped connecting plate, and the connecting rod can be movably located in the interval between the two U-shaped connecting plates; a screw hole is provided on the other side of the tube insertion seat, and a mounting hole corresponding to the position of the screw hole is provided on the other side of the docking seat, and the mounting member is a screw corresponding to the screw hole.

[0012] In one embodiment, the first pressing end surface is a plane, and the second pressing end surface is a plane.

[0013] In one embodiment, the connector tube assembly further includes a cannula connector provided between the connecting tube and the consumable pipeline, the cannula connector being used to be installed on the wall of the liver reservoir, and the connecting tube is connected to the consumable pipeline via the cannula connector.

[0014] In one embodiment, a side hole Luer connector is provided on the docking seat, and the side hole Luer connector is located on the side wall of the connecting tube and is connected to the connecting tube.

[0015] A method for connecting the joint pipe assembly comprises the following steps:

[0016] When the joint pipe assembly is connected to the artery of the ex vivo liver, the main trunk of the artery end is cut into sheets arranged circumferentially around the artery end, the artery is inserted into the through hole, the sheet at the artery end is laid flat on the first pressing end surface, and the first pressing end surface of the docking seat is pressed and matched with the second pressing end surface of the cannula insertion seat;

[0017] When the connector tube assembly is connected to the portal vein of an ex vivo liver, the main trunk of the portal vein end is cut into sheets arranged circumferentially around the arterial end, the portal vein is inserted into the through hole, the sheet at the portal vein end is laid flat on the first pressing end face, and the first pressing end face of the docking seat is pressed and matched with the second pressing end face of the intubation seat.

[0018] The connection method of the above-mentioned connector tube assembly is not to insert the cannula directly into the artery as in the traditional method. In this way, the inner wall of the artery is not touched during the perfusion process, thus avoiding damage to the endothelial tissue of the artery. At the same time, no suture operation is required, which greatly improves the assembly work efficiency. In addition, the connector tube assembly is firmly combined with the artery or portal vein of the isolated liver, and there is no risk of falling off during the perfusion process.

[0019] An isolated liver perfusion system comprises two of the above-mentioned connector pipe assemblies, a liver reservoir and consumable pipelines; the bottom of the liver reservoir is used to hold perfusion fluid, the liver reservoir is also used to place the isolated liver, one of the connector pipe assemblies is used to communicate with the artery of the isolated liver, the other connector pipe assembly is used to communicate with the portal vein of the isolated liver, and the connecting pipe is connected to the consumable pipeline.

[0020] The above-mentioned isolated liver perfusion system, since it includes the above-mentioned connector tube assembly, has the technical effects brought by the connector tube assembly, and the beneficial effects are the same as those of the connector tube assembly, which will not be described in detail here.

[0021] In one of the embodiments, the liver storage device is further provided with an openable cover, and the joint pipe assembly is located inside the liver storage device; the liver storage device comprises a bottom plate and a side plate connected to the bottom plate, the side plate is circumferentially arranged around the bottom plate, the side plate is a stepped plate, and the opening size of the side plate decreases from the top to the bottom.

[0022] In one embodiment, the liver storage device includes a bottom plate, which is a concave panel, a liquid conducting groove is provided at the bottom of the concave panel, a liquid outlet is provided on the bottom wall of the liquid conducting groove, the bottom wall of the liquid conducting groove is an inclined wall, the liquid outlet is provided at the lower part of the inclined wall, and a liquid outlet joint is provided at the liquid outlet; the concave surface of the concave panel is a semi-ellipsoidal surface or a hemispherical surface. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The drawings constituting a part of this application are used to provide a further understanding of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0025] Figure 1 It is a structural schematic diagram of a joint pipe assembly and an isolated liver combined together according to an embodiment of the present invention;

[0026] Figure 2 It is a structural schematic diagram of a joint pipe assembly according to an embodiment of the present invention;

[0027] Figure 3 It is a structural schematic diagram of a joint pipe assembly according to an embodiment of the present invention;

[0028] Figure 4This is a schematic structural diagram of a liver storage device according to an embodiment of the present invention when the cover is closed;

[0029] Figure 5 It is a structural schematic diagram of a liver storage device from one viewing angle after the cover of the liver storage device according to one embodiment of the present invention is removed;

[0030] Figure 6 It is a structural schematic diagram of a liver storage device according to an embodiment of the present invention after the cover is removed from another perspective;

[0031] Figure 7 It is a structural schematic diagram of a liver storage device from another perspective after the cover of the liver storage device according to one embodiment of the present invention is removed;

[0032] Figure 8 For Figure 7 A schematic diagram of a structure in which an air bag is placed;

[0033] Fig. 9 For Figure 8 A schematic diagram of the structure of an isolated liver placed on top of an air bag from one perspective;

[0034] Fig.10 For Figure 8 Another perspective structural diagram of an isolated liver placed on top of an air bag;

[0035] Fig.11 For Fig.10 Schematic diagram of the structure of placing a moisturizing membrane on the isolated liver;

[0036] Fig.12 FIG. 4 is a schematic structural diagram of an isolated liver perfusion system according to an embodiment of the present invention.

[0037] 10. Connector tube assembly; 11. Insertion seat; 111. Through hole; 112. First pressing end face; 113. Screw hole; 114. U-shaped connecting plate; 1141. Anti-drop flange; 12. Docking seat; 121. Second pressing end face; 122. Connecting tube; 123. Mounting hole; 124. Mounting piece; 125. Side hole Luer connector; 126. Connecting rod; 127. Rotating shaft; 20. Isolated liver; 21. Artery; 22. Sheet; 30. Liver storage device; 31. Cover; 311. Fastener; 32. Bottom plate; 321. Liquid guide groove; 322. Liquid outlet; 323. Liquid outlet Joint; 324, reinforcing rib plate; 33, side panel; 331, front side panel; 332, rear side panel; 333, left side panel; 334, right side panel; 341, 342, 343, 344, 345, through-plate joint; 35, inverted structure; 40, power pump; 50, membrane lung; 61, air-oxygen mixer; 62, oxygen tank; 63, carbon dioxide tank; 64, heat exchange device; 71, micro-embolus filter; 72, pressure sensor; 73, flow sensor; 74, host; 75, display screen; 76, power supply; 77, bile holding device; 81, air bag; 82, moisturizing membrane. DETAILED DESCRIPTION

[0038] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific embodiments disclosed below.

[0039] Generally, conventional cannulas corresponding to the liver artery and portal vein are usually in the shape of strips. After inserting the cannula into the liver artery or portal vein, medical staff still need to tie and fix it with surgical thread, which is cumbersome. There is a risk that the surgical thread is not tied firmly and the cannula will fall off during the perfusion process. In addition, during the process of inserting the cannula into the liver artery or portal vein, the portal vein or arterial endothelial tissue will be damaged.

[0040] Based on this, see Figures 1 to 3 , Figure 1 The schematic diagram of the structure of the joint pipe assembly 10 and the isolated liver 20 according to one embodiment of the present invention is shown. Figure 2 FIG. 1 is a schematic structural diagram of a joint pipe assembly 10 according to an embodiment of the present invention. Figure 3 The schematic diagram of the structure of a joint pipe assembly 10 according to an embodiment of the present invention is shown. An embodiment of the present invention provides a joint pipe assembly 10, which includes: a pipe inserting seat 11 and a docking seat 12.

[0041] The cannula seat 11 is provided with a through hole 111 for inserting the artery 21 or portal vein of the ex vivo liver 20, and the cannula seat 11 is provided with a first pressing end face 112, and the through hole 111 is located in the middle of the first pressing end face 112. The docking seat 12 is provided with a docking hole, and the docking hole and the through hole 111 are arranged in a corresponding position. The docking seat 12 is also provided with a second pressing end face 121. The docking hole is located in the middle of the second pressing end face 121, and the second pressing end face 121 is pressed and matched with the first pressing end face 112. The docking seat 12 is also provided with a connecting pipe 122 connected to the docking hole, and the connecting pipe 122 is used to be connected with the consumable pipeline.

[0042] The above-mentioned joint pipe assembly 10 can connect the artery 21 or portal vein of the ex vivo liver 20 to the consumables pipeline, so that the artery 21 or portal vein of the ex vivo liver 20 is connected to the perfusion circulation pipeline. Taking the connection of the artery 21 of the ex vivo liver 20 to the consumables pipeline as an example, the connection process of the artery 21 and the joint pipe assembly 10 is explained. The trunk at the end of the artery 21 is retained before perfusion, and the trunk at the end of the artery 21 is cut into a sheet 22 arranged circumferentially around the end of the artery 21. The artery 21 is inserted into the through hole 111, so that the sheet 22 at the end of the artery 21 is laid flat on the first pressing end face 112, and the first pressing end face 112 of the docking seat 12 is pressed and matched with the second pressing end face 121 of the cannula seat 11. At this time, the docking hole is connected to the artery 21. In addition, the connecting tube 122 is connected to the consumables pipeline, so that the artery 21 of the ex vivo liver 20 is connected to the consumables pipeline. After the ex vivo liver 20 is connected to the perfusion circulation pipeline, the perfusion work can be started. After the perfusion is completed, the docking seat 12 and the cannula seat 11 are separated, and the sheet 22 at the end of the artery 21 is trimmed to complete the perfusion. It can be seen that the cannula is not directly inserted into the artery 21 as in the traditional way. In this way, the inner wall of the artery 21 is not touched during the perfusion process, which avoids damage to the endothelial tissue of the artery 21. At the same time, no suture operation is required, which greatly improves the assembly work efficiency. In addition, the joint pipe assembly 10 is firmly combined with the artery 21 or portal vein of the ex vivo liver 20, and there is no risk of falling off during the perfusion process.

[0043] See also Figures 1 to 3 Furthermore, one side of the tube insertion seat 11 is movably connected to one side of the docking seat 12, and the other side of the tube insertion seat 11 is detachably connected to the other side of the docking seat 12 through the mounting member 124. In this way, since one side of the tube insertion seat 11 is movably connected to one side of the docking seat 12, when the tube insertion seat 11 and the docking seat 12 are opened and closed, the mounting member 124 is used to assemble the other side of the tube insertion seat 11 and the other side of the docking seat 12 together, so that the opening and closing operations between the tube insertion seat 11 and the docking seat 12 can be easily realized.

[0044] It should be noted that the "movable" in the "movable" connection in this embodiment is relative to "fixed". When two components are "movably" connected, it should be understood that the two components are not "fixedly" connected, but for example: rotationally connected, and / or movable in any direction, etc.

[0045] Specifically, one side of the docking seat 12 can not only move relative to one side of the intubation seat 11 in a direction perpendicular to the first pressing end face 112, but also rotate relative to one side of the intubation seat 11. In this way, when the docking seat 12 and the intubation seat 11 are in an open state, since one side of the docking seat 12 can move relative to one side of the intubation seat 11 in a direction perpendicular to the first pressing end face 112, the first pressing end face 112 and the second pressing end face 121 are in a relatively separated position, so that the first pressing end face 112 and the second pressing end face 121 can better fix the sheet 22 at the end of the artery 21.

[0046] In a specific embodiment, see Figure 3 Two U-shaped connecting plates 114 are arranged at intervals on one side of the intubation seat 11, and a connecting rod 126 and a rotating shaft 127 connected to the connecting rod 126 are arranged on one side of the docking seat 12. One end of the rotating shaft 127 is rotatably arranged in one of the U-shaped connecting plates 114 and can move along one of the U-shaped connecting plates 114, and the other end of the rotating shaft 127 is rotatably arranged in the other U-shaped connecting plate 114 and can move along the other U-shaped connecting plate 114. The connecting rod 126 is movably located in the interval between the two U-shaped connecting plates 114.

[0047] Furthermore, an anti-slip flange 1141 is provided at the mouth of the U-shaped connecting plate 114. The anti-slip flange 1141 is used to limit and interfere with the rotating shaft 127 to prevent the rotating shaft 127 from moving outward from the mouth of the U-shaped connecting plate 114. The U-shaped connecting plate 114 is specifically made of an elastic material, so that when a force is applied to the rotating shaft 127, the rotating shaft 127 can be moved outward from the mouth of the U-shaped connecting plate 114, that is, the intubation seat 11 and the docking seat 12 are separated for cleaning and maintenance. The intubation seat 11 and the docking seat 12 can also be assembled together by using the opposite operation steps.

[0048] It is understandable that, in order to realize that one side of the docking seat 12 can move relative to one side of the intubation seat 11 in a direction perpendicular to the first clamping end face 112, and can also rotate relative to one side of the intubation seat 11, it is not limited to the above-mentioned U-shaped connecting plate 114, connecting rod 126 and rotating shaft 127. The matching mode of one side of the intubation seat 11 and one side of the docking seat 12 can also adopt other design modes, which are not limited here. For example, one side of the intubation seat 11 is provided with a guide rail, and one side of the docking seat 12 is provided with a slider that cooperates with the guide rail, and the slider can be slidably arranged on the guide rail, so that the docking seat 12 can move relative to the intubation seat 11. For another example, when the rotating shaft 127 is rotatably connected to the connecting rod 126, the docking seat 12 can be rotated and closed on the intubation seat 11.

[0049] See also Figures 1 to 3 Furthermore, a screw hole 113 is provided on the other side of the intubation seat 11, and a mounting hole 123 corresponding to the position of the screw hole 113 is provided on the other side of the docking seat 12, and the mounting member 124 is a screw corresponding to the screw hole 113.

[0050] In an optional embodiment, the intubation seat 11 and the docking seat 12 are not connected in a combination manner in which one side is rotatably connected and the other side is detachably connected, but the intubation seat 11 as a whole is detachably connected to the docking seat 12. Specifically, the connection method between the intubation seat 11 and the docking seat 12 can be, for example, a snap connection, a riveted connection, a screw connection, a bolt connection, a pin connection, etc., which is not limited here.

[0051] See also Figures 1 to 3 In one embodiment, the first pressing end surface 112 is a plane, and the second pressing end surface 121 is a plane. In this way, when the first pressing end surface 112 and the second pressing end surface 121 are pressed together, the sheet 22 at the end of the artery 21 can be firmly pressed to ensure sealing. Optionally, the first pressing end surface 112 can also be an arcuate surface, and the second pressing end surface 121 is an arcuate surface corresponding to the first pressing end surface 112. In addition, the first pressing end surface 112 can also be a surface of other shapes, which is not limited here. As long as the second pressing end surface 121 is adapted to the shape of the first pressing end surface 112, the sheet 22 at the end of the artery 21 can be firmly pressed when the first pressing end surface 112 and the second pressing end surface 121 are pressed together.

[0052] See also Figures 1 to 3 In one embodiment, the connector tube assembly 10 further includes a cannula connector disposed between the connecting tube 122 and the consumables pipeline (specifically corresponding to Figure 4The two through-plate connectors 341 are provided in the liver storage device 30. The cannula connector is used to be installed on the wall of the liver storage device 30, and the connecting tube 122 is connected to the consumables pipeline through the cannula connector. In this way, it is convenient to connect the connecting tube 122 inside the liver storage device 30 to the consumables pipeline outside the liver storage device 30.

[0053] See also Figures 1 to 3 In one embodiment, a side hole Luer connector 125 is provided on the docking seat 12. The side hole Luer connector 125 is located on the side wall of the connecting tube 122 and communicates with the connecting tube 122. In this way, the pressure tube can be directly connected to the side hole Luer connector 125. The pressure connector is arranged on the docking seat 12, which is very close to the position of the portal vein or artery 21, and can more truly measure the pressure of the portal vein or artery 21, avoiding the installation of a connector in the middle of the circulation pipeline to connect the pressure tube, which is far away from the portal vein or artery 21, resulting in a pressure difference and causing a large measurement error.

[0054] In one embodiment, a connection method of a joint pipe assembly 10 according to any of the above embodiments comprises the following steps:

[0055] When the joint tube assembly 10 is connected to the artery 21 of the ex vivo liver 20, the main trunk at the end of the artery 21 is cut into a sheet 22 circumferentially arranged around the end of the artery 21, and the artery 21 is inserted into the through hole 111, so that the sheet 22 at the end of the artery 21 is laid flat on the first pressing end surface 112, and the first pressing end surface 112 of the docking seat 12 is pressed and matched with the second pressing end surface 121 of the cannula seat 11; at this time, the docking hole is connected to the artery 21, and the connecting tube 122 is connected to the consumable pipeline, so that the artery 21 of the ex vivo liver 20 is connected to the consumable pipeline.

[0056] When the joint tube assembly 10 is connected to the portal vein of the ex vivo liver 20, the main trunk of the portal vein end is cut into a sheet 22 circumferentially arranged around the end of the artery 21, and the portal vein is inserted into the through hole 111, so that the sheet 22 at the end of the portal vein is laid flat on the first pressing end surface 112, and the first pressing end surface 112 of the docking seat 12 is pressed and matched with the second pressing end surface 121 of the cannula seat 11. At this time, the docking hole is connected to the portal vein, and the connecting tube 122 is connected to the consumables pipeline, so that the portal vein of the ex vivo liver 20 is connected to the consumables pipeline.

[0057] The connection method of the above-mentioned connector tube assembly 10 is not to insert the cannula directly into the artery 21 as in the traditional method. In this way, the inner wall of the artery 21 is not touched during the perfusion process, thus avoiding damage to the inner wall cells of the artery 21. At the same time, no suture operation is required, which greatly improves the assembly work efficiency. In addition, the connector tube assembly 10 is firmly combined with the artery 21 or portal vein of the isolated liver 20, and there is no risk of falling off during the perfusion process.

[0058] It should be noted that the above-mentioned joint pipe assembly 10 can be used in closed-type perfusion as well as in open-loop perfusion. The following will specifically describe the use of the joint pipe assembly 10 in open-loop perfusion as an example.

[0059] See also Fig.12 , Fig.12 The schematic diagram of the structure of the perfusion system of an isolated liver 20 according to an embodiment of the present invention is shown. In one embodiment, a perfusion system of an isolated liver 20 includes two connector tube assemblies 10 of any of the above embodiments, and also includes a liver reservoir 30 and consumables pipelines. The bottom of the liver reservoir 30 is used to install the perfusion fluid, and the liver reservoir 30 is also used to place the isolated liver 20. One of the connector tube assemblies 10 is used to communicate with the artery 21 of the isolated liver 20, and the other connector tube assembly 10 is used to communicate with the portal vein of the isolated liver 20, and the connecting tube 122 is connected to the consumables pipeline.

[0060] The above-mentioned isolated liver 20 perfusion system, since it includes the above-mentioned connector tube assembly 10, its technical effects are brought by the connector tube assembly 10, and the beneficial effects are the same as the beneficial effects of the connector tube assembly 10, which will not be repeated here.

[0061] In addition, it can be understood that there are two consumable pipelines, and the two consumable pipelines are set in one-to-one correspondence with the artery 21 and portal vein of the isolated liver 20. One of the consumable pipelines, the artery 21 of the isolated liver 20 and the liver reservoir 30 form a perfusion circulation loop, and the perfusion liquid flows out through the liver reservoir 30 into the consumable pipeline, and circulates through the consumable pipeline to the artery 21 of the isolated liver 20, and is discharged from the isolated liver 20 to the liver reservoir 30, and so on. The other consumable pipeline, the portal vein of the isolated liver 20 and the liver reservoir 30 form a perfusion circulation loop, and the perfusion liquid flows out through the liver reservoir 30 into the consumable pipeline, and circulates through the consumable pipeline to the portal vein of the isolated liver 20, and is discharged from the isolated liver 20 to the liver reservoir 30, and so on.

[0062] See also Fig.12 Furthermore, a power pump 40 and a membrane lung 50 are provided on the consumable pipeline. Under the action of the power pump 40, the perfusion fluid of the liver reservoir 30 enters the membrane lung 50, and then enters the artery 21 or portal vein of the isolated liver 20 after passing through the membrane lung 50.

[0063] See also Fig.12In one embodiment, the membrane lung 50 is provided with a mixed gas interface for communicating with a mixed gas source pipeline. The perfusion liquid is combined with the mixed gas in the membrane lung 50 to form an oxygenated perfusion liquid to ensure that the oxygen content of the perfusion liquid flowing into the artery 21 is sufficient. Specifically, the mixed gas source is provided by an air-oxygen mixer 61, and the air-oxygen mixer 61 is connected to an oxygen tank 62 and a carbon dioxide tank 63 through pipelines, and the air-oxygen mixer 61 is connected to the mixed gas interface. The oxygen from the oxygen tank 62 and the carbon dioxide from the carbon dioxide pipe are mixed in the air-oxygen mixer 61 to obtain a mixed gas source, and the mixed gas source is delivered to the membrane lung 50 through the mixed gas interface.

[0064] See also Fig.12 In addition, the membrane lung 50 is connected to the heat exchange device 64 through a heat exchange pipe to form a circulating heat exchange loop. The perfusion fluid exchanges heat with the heat exchange medium of the circulating heat exchange loop in the membrane lung 50 to keep the temperature of the perfusion fluid suitable for the isolated organ. The heat exchange device 64 is specifically, for example, a constant temperature water tank, so that the temperature of the perfusion fluid is maintained at a preset temperature, for example, 37°C.

[0065] See also Fig.12 Furthermore, a microembolism filter 71 is also provided on the consumables pipeline, and the microembolism filter 71 can be provided on the pipeline connected to the liquid inlet end of the membrane lung 50, or on the pipeline connected to the liquid outlet end of the membrane lung 50. In this way, the microembolism filter 71 is used to filter out various microemboli in the perfusion fluid, prevent embolism of microvessels of isolated organs caused by various microemboli such as thrombus or air embolism, effectively improve the blood perfusion of human microvessels, and further improve the utilization rate of donor organs and the success rate of transplantation surgery.

[0066] See also Fig.12 Furthermore, a pressure sensor 72 is provided on the consumables pipeline. The pressure sensor 72 is provided at the liquid outlet of the membrane lung 50 and close to the portal vein or artery 21 to measure the pressure of the portal vein or artery 21. Figures 1 to 3 , a side hole Luer connector 125 is provided on the docking seat 12. The side hole Luer connector 125 is located on the side wall of the connecting tube 122 and is connected to the connecting tube 122. In this way, the pressure sensor 72 is directly connected to the side hole Luer connector 125 through the pressure tube. The pressure connector is located on the docking seat 12, and is very close to the position of the portal vein or artery 21. In this way, the pressure of the portal vein or artery 21 can be measured more realistically, avoiding the installation of a connector in the middle of the circulation pipeline to connect the pressure tube, which is far away from the portal vein or artery 21, resulting in a pressure difference and causing a large measurement error.

[0067] See also Fig.12Furthermore, a flow sensor 73 is also provided on the consumables pipeline. The flow sensor 73 is arranged on the pipeline between the power pump 40 and the microembolic filter 71 or on the liquid outlet of the membrane lung 50. The specific arrangement position is not limited here. The flow sensor 73 can obtain the flow rate of the perfusion liquid on the consumables pipeline, and can judge whether the power pump 40, the microembolic filter 71, and the membrane lung 50 are working normally according to the flow rate.

[0068] See also Fig.12 Furthermore, the above-mentioned isolated liver 20 perfusion system also includes a host 74, a display screen 75 and a power supply 76. The power supply 76 is electrically connected to the host 74, the display screen 75, the power pump 40 and the heat exchange device 64 respectively, and the host 74 is electrically connected to the display screen 75, the heat exchange device 64, the pressure sensor 72 and the flow sensor 73 respectively. The host 74 can control the display screen 75, the heat exchange device 64, the pressure sensor 72 and the flow sensor 73 to work accordingly, and the display screen 75 is used to display the detection data of the pressure sensor 72 and the flow sensor 73.

[0069] See also Figure 4 , Figure 4 The structure diagram of the liver storage device 30 when the cover 31 is closed according to an embodiment of the present invention is illustrated. In one embodiment, the liver storage device 30 is further provided with an openable cover 31. The joint pipe assembly 10 is located inside the liver storage device 30. In this way, when the ex vivo liver 20 needs to be taken in and out, the cover 31 is opened. When the ex vivo liver 20 is placed in the liver storage device 30 for perfusion, the cover 31 is closed to seal the liver storage device 30, thereby playing a protective and moisturizing role. The cover 31 is detachably connected to the mouth of the liver storage device 30, for example, by using a plurality of fasteners 311.

[0070] See also Figures 5 to 7 , Figure 5 The structure diagram of the liver storage device 30 in one embodiment of the present invention after the cover 31 is removed is shown in one viewing angle. Figure 6 The structure diagram of the liver storage device 30 according to one embodiment of the present invention after the cover 31 is removed from another perspective is shown. Figure 7 The structure schematic diagram of the liver storage device 30 of one embodiment of the present invention after the cover 31 is removed from another perspective is shown. In addition, the liver storage device 30 includes a bottom plate 32 and a side plate 33 connected to the bottom plate 32. The side plate 33 is arranged circumferentially around the bottom plate 32, and the side plate 33 is a stepped plate, and the opening size of the side plate 33 decreases from the top to the bottom. In this way, the opening size of the top of the side plate 33 is larger than the opening size of the bottom of the side plate 33, and the side plate 33 is stepped, so that there is enough space at the top position of the liver storage device 30 to place the connecting joint, the joint pipe assembly 10 and related pipelines.

[0071] See also Figures 5 to 7In one embodiment, the liver storage device 30 includes a bottom plate 32. The bottom plate 32 is a concave panel. Furthermore, a liquid guide groove 321 is provided at the bottom of the concave panel, and a liquid outlet 322 is provided on the bottom wall of the liquid guide groove 321. The bottom wall of the liquid guide groove 321 is an inclined wall arranged obliquely, and the liquid outlet 322 is arranged at the lower part of the inclined wall. A liquid outlet connector 323 is provided at the liquid outlet 322. Specifically, there are two liquid outlets 322, and there are two liquid outlet connectors 323. The two liquid outlet connectors 323 are arranged one by one at the two liquid outlets 322, and one of the liquid outlet connectors 323 introduces the perfusion liquid into the consumable pipeline corresponding to the artery 21, and the other liquid outlet connector 323 introduces the perfusion liquid into the consumable pipeline corresponding to the portal vein.

[0072] In this way, all the perfusion fluids can participate in the blood circulation during the perfusion process, and there is no accumulating fluid in the container in each cycle. The design of the liquid guide groove 321 can more effectively guide the discharge of the perfusion fluid during the perfusion process, avoid the formation of thrombus by accumulating fluid, and the liquid outlet joint 323 is arranged at the lowest point of the low liquid guide groove 321, so that the perfusion fluid can quickly flow out of the liver storage device 30 for the next blood circulation. Since the matching liver perfusion mode is a liver open-loop perfusion mode, that is, the perfusion fluid passing through the liver is directly discharged into the liver storage device 30, and the liquid outlet 322 of the liver storage device 30 guides the liquid for the next round of perfusion cycle. In order to avoid empty pumping during the perfusion process (empty pumping means that the liquid flow rate flowing out of the isolated liver 20 at the same time is less than the flow rate flowing out of the liver storage device 30), the perfusion fluid needs to store a certain amount of stable perfusion fluid at the bottom of the liver storage device 30 to cover the liquid outlet 322 for a long time, so that it can be replenished in time when there may be a difference in the amount of perfusion fluid flowing out of the liver storage container and the liver.

[0073] Specifically, the concave surface of the concave panel is a semi-elliptical spherical surface or a hemispherical surface. It should be noted that the concave panel is not limited to the semi-elliptical spherical surface or the hemispherical surface in the present embodiment, and can also be other shapes, which are not limited here. In this way, the bottom design scheme of the semi-elliptical spherical surface or the hemispherical surface, the horizontal cross-sectional area on the vertical height is gradually reduced from top to bottom, so the volume of the lowest part is the smallest, and a lot of perfusion liquid is saved compared to the design of equal cross-sectional area on the vertical height. That is, it can avoid that there are more effusions in the corners of the liver storage device 30, avoid that the liver storage device 30 with a larger bottom area consumes more perfusion liquid during the perfusion process, and can realize that the perfusion liquid in the liver storage device 30 is gathered and concentrated in the liquid guide groove 321, and discharged outwardly into the consumables pipeline through the liquid outlet joint 323. In addition, when the concave surface of the concave panel is a semi-elliptical spherical surface or a hemispherical surface, it can be realized that the perfusion liquid can be better gathered and concentrated in the bottom of the liquid guide groove 321.

[0074] Furthermore, a reinforcing rib plate 324 is provided on the bottom surface of the bottom plate 32 . The reinforcing rib plate 324 is divided into a transversely arranged reinforcing rib plate 324 and a longitudinally arranged reinforcing rib plate 324 . The reinforcing rib plate 324 is equivalent to the base of the liver storage device.

[0075] Generally, during the perfusion process, the ex vivo liver 20 is supported by the bottom of the liver storage device 30, and the support surface is in contact with the surface of the ex vivo liver 20. The contacting portion of the liver surface is under pressure, which can easily cause poor blood circulation in the liver during the perfusion process, thereby causing thrombosis.

[0076] See also Figures 8 to 10 , Figure 8 Indicated in Figure 7 A schematic diagram of the structure in which the air bag 81 is placed, Fig. 9 Indicated in Figure 8 A schematic structural diagram of one viewing angle of the isolated liver 20 placed on the air bag 81, Fig.10 Indicated in Figure 8 Another perspective structural diagram of placing the ex vivo liver 20 on the airbag 81. Further, an airbag 81 is provided at the bottom of the liver reservoir 30. In this way, by adding the airbag 81 at the bottom of the liver reservoir 30, the ex vivo liver 20 is placed on the airbag 81, and the breathing of the human lung is simulated during perfusion, and the airbag 81 is evacuated and inhaled, so that the airbag 81 continues to relax during the perfusion process, and the contact point between the ex vivo liver 20 and the airbag 81 is also changing, so that the blood circulation of the ex vivo liver 20 is smoother during the perfusion process, and thrombosis is avoided on the surface of the ex vivo liver 20 due to long-term fixed pressure.

[0077] See also Figure 8 Further, the airbag 81 is specifically an elliptical ring, and its outermost elliptical curve is consistent with the cross-sectional elliptical curve of the elliptical surface of the concave panel, so that the airbag 81 can be placed in the concave panel, and the airbag 81 has a good fixing effect in the concave panel. There is a hole in the middle of the airbag 81, and the perfusion fluid discharged from the ex vivo liver 20 can pass through the hole and flow into the liquid guide groove 321 at the bottom of the liver storage device 30.

[0078] See also Figures 9 to 11 , Fig.11 Indicated in Fig.10A schematic diagram of the structure of placing a moisturizing membrane 82 above the ex vivo liver 20. Furthermore, the ex vivo liver 20 perfusion system also includes a moisturizing membrane 82. The moisturizing membrane 82 is used to be arranged above the ex vivo liver 20. In this way, during the mechanical perfusion of the ex vivo liver 20, the moisturizing membrane 82 covers the top of the ex vivo liver 20 to prevent the loss of water in the portal vein, artery 21 and liver surface during the perfusion process. In addition, the moisturizing membrane 82 is fixedly matched with the inverted structure 35 provided on the liver reservoir 30, and is fixed on the liver reservoir 30, so that the ex vivo liver 20 can be limited to a certain extent on the vertical plane to prevent the ex vivo liver 20 from moving upward.

[0079] Please refer to Figures 9 to 11 , further, a plurality of through-plate joints (341, 342, 343, 344, 345) are provided on the side plate 33. The through-plate joints (341, 342, 343, 344, 345) can realize the corresponding connection between the connecting tube 122, the side hole Luer joint 125, and the air bag 81 inside the liver storage device 30 and the external structure. Specifically, two of the through-plate joints 341 are cannula joints, and the connecting tube 122 is correspondingly connected to the cannula joints through a hose, and the cannula joints are connected to the consumables pipeline through a pipeline (such as a hose). Two of the through-plate joints 342 are pressure pipe joints, and the side hole Luer joint 125 is connected to the pressure pipe joint through a pipeline (such as a hose), and the pressure pipe joint is connected to the pressure sensor through a pipeline (such as a hose), and the pressure sensor correspondingly senses the pressure at the side hole Luer joint 125. The two through-plate connectors 343 are tracheal connectors, and the air inlet and the air outlet of the airbag 81 are respectively connected to the two tracheal connectors, and the two tracheal connectors are connected to external gas transmission equipment. In addition, one of the through-plate connectors 344 is a bile tube connector, and the bile duct of the isolated liver 20 is connected to the bile tube connector through a pipeline (such as a hose), and the bile duct of the isolated liver 20 discharges bile to the outside world through the hose and the bile tube connector to the bile containing device 77 (such as Fig.12 The redundant through-plate connector 345 is a spare connector.

[0080] See also Figures 5 to 7 as well as Fig.11 Further, the side panel 33 includes a front side panel 331, a rear side panel 332, a left side panel 333 and a right side panel 334. The front side panel 331 and the rear side panel 332 are arranged opposite to each other, and the left side panel 333 and the right side panel 334 are arranged opposite to each other. The front side panel 331, the rear side panel 332, the left side panel 333 and the right side panel 334 are respectively surrounded by the four sides of the bottom panel 32. The front side panel 331 is in a two-step shape, and the cross section of the upper step is wider than the cross section of the lower step. Joint holes are provided on the upper and lower step vertical surfaces of the front side panel 331, and the through-plate joint is installed in the joint hole. Two joint holes are also provided on the left side panel 333.

[0081] See also Figures 5 to 7 as well as Fig.11 , further, there are four inverted structures 35, two of which are arranged on the stepped bottom plate of the front side plate 331, and the other two are arranged on the horizontal bottom plate of the upper surface of the elliptical surface of the liver storage device 30, and the arranged position is close to the rear side plate 332. In this way, one side of the moisturizing membrane 82 is connected to the two inverted structures 35 on the stepped bottom plate of the front side plate 331, and the other side of the moisturizing membrane 82 is connected to the two inverted structures 35 on the horizontal bottom plate of the upper surface of the elliptical surface of the liver storage device 30. The fixed position of one side of the moisturizing membrane 82 is higher than the fixed position of the other side of the moisturizing membrane 82, which can achieve a better fixing effect on the ex vivo liver 20.

[0082] The technical features of the above embodiments may be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0083] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

[0084] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0085] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0086] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0087] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0088] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used herein are for illustrative purposes only and are not intended to be the only implementation method.

Claims

1. A joint pipe assembly, characterized in that: The joint pipe assembly comprises: A cannula seat, wherein the cannula seat is provided with a through hole for inserting an artery or portal vein of an isolated liver, and the cannula seat is provided with a first pressing end surface, wherein the through hole is located in the middle of the first pressing end surface; A docking seat, wherein the docking seat is provided with a docking hole, wherein the docking hole is arranged corresponding to the position of the through hole, wherein the docking seat is also provided with a second clamping end face, wherein the docking hole is located in the middle of the second clamping end face, wherein the second clamping end face is clamped and matched with the first clamping end face, wherein the docking seat is also provided with a connecting pipe connected to the docking hole, wherein the connecting pipe is used to be connected to the consumable pipeline; wherein one side of the intubation seat is movably connected to one side of the docking seat, and the other side of the intubation seat is detachably connected to the other side of the docking seat through a mounting piece; wherein two U-shaped connecting plates are arranged at intervals on one side of the intubation seat , a connecting rod and a rotating shaft connected to the connecting rod are provided on one side of the docking seat, one end of the rotating shaft is rotatably arranged in one of the U-shaped connecting plates and can move along one of the U-shaped connecting plates, the other end of the rotating shaft is rotatably arranged in the other U-shaped connecting plate and can move along the other U-shaped connecting plate, and the connecting rod can be movably located in the interval between the two U-shaped connecting plates; a screw hole is provided on the other side of the tube insertion seat, and a mounting hole corresponding to the position of the screw hole is provided on the other side of the docking seat, and the mounting piece is a screw corresponding to the screw hole.

2. The joint pipe assembly according to claim 1, characterized in that: The mouth of the U-shaped connecting plate is provided with an anti-slip flange; the anti-slip flange is used for limiting and interfering with the rotating shaft.

3. The joint pipe assembly according to claim 2, characterized in that: The U-shaped connecting plate is made of elastic material.

4. The joint pipe assembly according to claim 1, characterized in that: The first pressing end surface is a plane, and the second pressing end surface is a plane.

5. The joint pipe assembly according to claim 1, characterized in that: The connector pipe assembly also includes a cannula connector provided between the connecting pipe and the consumable pipeline, the cannula connector is used to be installed on the wall of the liver reservoir, and the connecting pipe is connected to the consumable pipeline through the cannula connector.

6. The joint pipe assembly according to any one of claims 1 to 5, characterized in that: The docking seat is provided with a side hole Luer connector, and the side hole Luer connector is located on the side wall of the connecting tube and is connected with the connecting tube.

7. A method for connecting a joint pipe assembly according to any one of claims 1 to 6, characterized in that: The steps include: When the joint pipe assembly is connected to the artery of the ex vivo liver, the main trunk of the artery end is cut into sheets arranged circumferentially around the artery end, the artery is inserted into the through hole, the sheet at the artery end is laid flat on the first pressing end surface, and the first pressing end surface of the docking seat is pressed and matched with the second pressing end surface of the cannula insertion seat; When the connector tube assembly is connected to the portal vein of an ex vivo liver, the main trunk of the portal vein end is cut into sheets arranged circumferentially around the arterial end, the portal vein is inserted into the through hole, the sheet at the portal vein end is laid flat on the first pressing end face, and the first pressing end face of the docking seat is pressed and matched with the second pressing end face of the intubation seat.

8. An isolated liver perfusion system, characterized in that: It comprises two connector tube assemblies according to any one of claims 1 to 6, and also comprises a liver reservoir and consumable pipelines; the bottom of the liver reservoir is used to hold perfusion fluid, and the liver reservoir is also used to place an isolated liver, one of the connector tube assemblies is used to be connected to an artery of the isolated liver, and the other connector tube assembly is used to be connected to a portal vein of the isolated liver, and the connecting tube is connected to the consumable pipeline.

9. The isolated liver perfusion system according to claim 8, characterized in that: The liver storage device is also provided with an openable cover body, and the joint pipe assembly is located inside the liver storage device; the liver storage device includes a bottom plate and a side plate connected to the bottom plate, the side plate is circumferentially arranged around the bottom plate, the side plate is a stepped plate, and the opening size of the side plate decreases from the top to the bottom.

10. The isolated liver perfusion system according to claim 8, characterized in that: The liver storage device comprises a bottom plate, which is a concave panel. A liquid conducting groove is provided at the bottom of the concave panel. A liquid outlet is provided on the bottom wall of the liquid conducting groove. The bottom wall of the liquid conducting groove is an inclined wall arranged obliquely. The liquid outlet is arranged at the lower part of the inclined wall. A liquid outlet joint is provided at the liquid outlet. The concave surface of the concave panel is a semi-ellipsoidal surface or a hemispherical surface.

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