A microscopic imaging system and a dark-field illuminator for microscopic imaging

Abstract

The present invention relates to a microscopic imaging system, comprising: a plurality of unit microscopic imaging modules arranged according to a preset rule, and a data acquisition card connected to the plurality of unit microscopic imaging modules; wherein each unit microscopic imaging module respectively includes an adjustable lens group capable of independently adjusting focus, and a photosensitive module corresponding to the adjustable lens group; a plurality of image processing modules respectively corresponding to the plurality of unit microscopic imaging modules are provided on the data acquisition card; each image processing module independently controls the focusing of the corresponding adjustable lens group and acquires the data of the corresponding photosensitive module. Implementing the present invention can reduce the requirement for the consistency of unit module assembly and improve the production efficiency of its finished products.

Description

Technical Field

[0001] The present invention belongs to the technical field of optical microscopy imaging, and particularly relates to a dark-field illuminator for microscopy imaging, and a microscopy imaging system including the dark-field illuminator for microscopy imaging. Background Art

[0002] With the development of optical imaging technology, the application fields of optical imaging modules are becoming more and more extensive, and there are more and more products in the fields of super-macro imaging and microscopy imaging. With the application development of artificial intelligence technology in the field of image processing and the mass production of high-performance image processing hardware, microscopy imaging technology is developing in the technical directions of large field of view, high resolution, high throughput, etc. For example, in the wafer inspection technology in the field of industrial inspection and the cervical screening technology in the field of cancer screening, it is usually necessary to image samples of centimeter scale with sub-micron resolution. At the same time, with the development of mobile Internet technology, portable intelligent mobile terminals have been popularized. Integrating traditional large-volume desktop detection instruments into portable terminals will be an important development direction in the future.

[0003] The traditional single-microscope-objective imaging scheme is limited by the manufacturing process and the size of the image sensor, and cannot obtain micron-level image resolution within a centimeter-scale imaging field of view. At present, the method for microscopy imaging of large-area samples is to move the optical system or the observed sample on the basis of a traditional microscope. For example, in the Chinese invention patent publication document with the application number CN201180009191.2, the solution is to add an electromechanical system such as a guide rail or a curved arm to move the optical system. In the Chinese utility model patent with the application number CN201420420879.0 and the Chinese invention patent with the application number CN201610746297.5, the method is to use an added electromechanical device or a manual device to move the placement platform of the observed sample. Both of the above two methods can obtain micron-level resolution images of centimeter-scale samples. However, the traditional microscopy imaging scheme based on a single objective can only obtain images of a large field of view in a serial manner, and is limited by traditional microscopy optical imaging devices. The complexity of the whole system is relatively high, the stability is relatively low and the price is expensive.

[0004] The method of increasing the overall observation area by observing different regions of the sample through an array of multiple objective lenses can also improve the efficiency of sample detection. The array - type solution can achieve large - field - of - view and high - resolution imaging in a parallel imaging mode. However, traditional microscope objective lenses are relatively large in volume, and the array mode will lead to a complex imaging structure, large volume, and high cost. The Chinese invention patent with the application number CN201910585599.2 discloses a novel objective lens array applied to multi - field parallel imaging, and its function realization mainly depends on the small microscope objective lens unit with large field - of - view and high performance mentioned in its disclosure document. The Chinese invention patent with the application number CN201910743158.0 discloses an array - type microscopic image acquisition system with a transmission illumination source, and the Chinese invention patent with the application number CN201910743162.7 discloses an array - type microscopic image acquisition system with a reflective illumination source. This type of imaging solution realizes an array solution with small volume and low cost by changing the structure of traditional microscope objective lenses. However, for an optical imaging system, the accuracy of focusing is the key to ensuring imaging quality. Usually, the depth of field of a high - resolution microscopic imaging system is within dozens of micrometers. For a centimeter - level field of view, ensuring the flatness within dozens of micrometers requires extremely high requirements for the design, processing, assembly, operation, and stability of the imaging system. Moreover, for some samples with large undulations, such as stacked cell samples, the sample itself has unevenness. Even if the array - type imaging system has achieved parfocal design, it is impossible to complete clear imaging of such samples in a single imaging process. It can be seen that due to the relatively large size of the traditional microscope eyepiece body relative to the sample, and the lack of an adjustable - focus micro - microscope product or combined method suitable for this situation, in the existing technical solutions, this type of focusing problem has not been well solved.

[0005] In addition, in the traditional solution with the application number CN201790000885.2, both the dark - field illuminator and the bright - field illuminator have their own light sources, and the bright - field and dark - field are independently separated, resulting in a complex imaging structure, large volume, and inconvenient switching between the bright - field and dark - field, making it difficult to be used in the field of portable microscopic imaging. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to provide a microscopic imaging system for the above - mentioned partial technical defects of the prior art.

[0007] The technical solution adopted by the present invention to solve its technical problems is: constructing a microscopic imaging system, including:

[0008] A plurality of unit microscopic imaging modules arranged according to a preset rule, and a data acquisition card connected to the plurality of unit microscopic imaging modules;

[0009] Wherein, each of the unit microscopic imaging modules respectively includes an adjustable lens group with independently adjustable focusing, and a photosensitive module corresponding to the adjustable lens group;

[0010] The data acquisition card is provided with a plurality of image processing modules respectively corresponding to the plurality of unit microscopic imaging modules one by one; each of the image processing modules independently controls the focusing of the corresponding adjustable lens group and acquires the data of the corresponding photosensitive module.

[0011] Optionally, the microscopic imaging system further includes a fixing mechanism disposed on the data acquisition card, and each of the adjustable lens groups is independently fixed to the data acquisition card through the fixing mechanism.

[0012] Optionally, the adjustable lens group includes a second lens group disposed close to the photosensitive module and a first lens group disposed far from the photosensitive module;

[0013] Each of the unit microscopic imaging modules includes a focusing motor, and the focusing motor is connected to any one of the first lens group and the second lens group, and can adjust the relative position of the first lens group and the second lens group to achieve the focusing of the adjustable lens group.

[0014] Optionally,

[0015] The focusing motor and the any one lens group connected thereto are encapsulated together as an integrated package module.

[0016] Optionally, the other lens group of the first lens group and the second lens group is fixedly connected to the fixing mechanism; or

[0017] The fixing mechanism includes a lens group fixing seat, and the other lens group of the first lens group and the second lens group is threadedly connected to the lens group fixing seat.

[0018] Optionally, a microscopic imaging system of the present invention further includes an illumination module corresponding to the plurality of unit microscopic imaging modules, and the illumination module includes:

[0019] A first illumination light source disposed above the unit microscopic imaging module; or

[0020] A second illumination light source uniformly disposed around each of the unit microscopic imaging modules, and a light guide structure fixed above the second illumination light source.

[0021] Optionally,

[0022] The first illumination light source includes a fluorescence excitation light source, and the unit microscopic imaging module further includes a fluorescence excitation filter disposed on the lower end face, inside or upper end face of the adjustable lens group; or

[0023] The first illumination light source includes a first dark-field illuminator disposed above the adjustable lens group and corresponding to the adjustable lens group;

[0024] The second illumination light source further includes a second dark-field illuminator disposed above the adjustable lens group and corresponding to the adjustable lens group.

[0025] Optionally,

[0026] The first dark-field illuminator includes an array of LED light sources with a first circular through-hole in the housing or a white backlight source with a second circular through-hole in the housing, and the first circular through-hole and the second circular through-hole are disposed opposite to the adjustable lens group;

[0027] The second dark-field illuminator includes a bright-dark field substrate and a dark-field black background patch, the dark-field black background patch is matched with the size of the adjustable lens group, and the dark-field black background patch is disposed closer to or farther from the adjustable lens group relative to the bright-dark field substrate.

[0028] Optionally, the white backlight source includes:

[0029] Hole-backlight sources disposed on both sides above the adjustable lens group, and the opening direction of the hole-backlight sources is parallel to the upper surface of the adjustable lens group; and / or

[0030] A black diffused surface semi-transparent thin sheet disposed directly above the adjustable lens group; and / or

[0031] A complete backlight source disposed on the side of the black diffused surface semi-transparent thin sheet away from the adjustable lens group.

[0032] Optionally,

[0033] The image processing module includes an image signal processing unit, a data buffer unit, a motor control unit, and a data transmission interface;

[0034] The image signal processing unit and the motor control unit are respectively and correspondingly connected to the photosensitive module and the focusing motor through a flexible cable;

[0035] The microscopic imaging system further includes a main controller and an image display unit, the main controller is connected to the image processing unit through the data buffer unit, and the main controller is connected to the image signal processing unit through a first bus and to the image display unit through a second bus.

[0036] Optionally,

[0037] The main controller and the image display unit are integrated into an intelligent terminal.

[0038] To achieve the above object, according to one aspect of the present invention, there is also provided a dark field illuminator for microscopic imaging (in the specific implementation part, especially in Examples 7-8 and Figures 15 - 16 in, specifically the second dark field illuminator). The dark field illuminator is disposed above the adjustable lens group of the unit microscopic imaging module corresponding to the adjustable lens group. The surface of the dark field illuminator is attached to the back of the sample slide. The sample slide is located between the dark field illuminator and the adjustable lens group;

[0039] The dark field illuminator includes a bright and dark field substrate and a dark field black background patch. The dark field black background patch is matched with the size of the adjustable lens group, and the dark field black background patch is arranged closer to or farther from the adjustable lens group relative to the bright and dark field substrate.

[0040] Optionally, in the case of dark field illumination, the entire surface of the dark field black background patch is attached to the back of the sample slide.

[0041] Optionally, the dark field illuminator is a reflective dark field illuminator, which is a white diffuser with the dark field black background patch.

[0042] Optionally, the white diffuser surrounds the dark field black background patch.

[0043] Optionally, the dark field black background patch is circular.

[0044] Optionally, the size of the dark field black background patch is larger than the field of view of the unit microscopic imaging module.

[0045] Optionally, the center of the dark field black background patch, the center of the sample, and the optical axis of the unit microscopic imaging module are located on the same axis.

[0046] Optionally, the bright and dark field substrate of the dark field illuminator further has a recessed structure, and the recessed structure has a white diffused surface, so that the dark field illuminator also has a bright field illumination function.

[0047] Optionally, in the case of bright field illumination, the surface of the bright and dark field substrate on the side of the recessed structure is attached to the back of the sample slide, and the opening of the recessed structure faces the unit microscopic imaging module.

[0048] Optionally, the dark field black background patch and the recessed structure are respectively arranged on the front and back sides of the bright and dark field substrate.

[0049] The above preferred technical features can be combined with each other as long as they do not conflict with each other.

[0050] Generally speaking, compared with the prior art, the above technical solutions conceived by the present invention have the following beneficial effects:

[0051] 1. The microscopic imaging system of the present invention has an independently adjustable focus design, which reduces the requirement for the consistency of unit module assembly and improves the production efficiency of its finished products.

[0052] 2. The dark-field illuminator of the microscopic imaging of the present invention does not have its own light source, but uses the light source of the unit microscopic imaging module, which reduces the volume. Moreover, it can be used alone as a dark-field illuminator, and also has a bright-field illumination structure and bright-field transmission illumination function, and can be used alone as a bright-field illuminator. It can also be switched between dark-field illumination and bright-field illumination. The switching between bright and dark fields is fast and convenient, realizing the integration and miniaturization of the dark-field illuminator and the bright-field illuminator. BRIEF DESCRIPTION OF THE DRAWINGS

[0053] Figure 1 is a schematic structural diagram of a first embodiment of a microscopic imaging system of the present invention;

[0054] Figure 2 is Figure 1 a schematic structural diagram of a first embodiment of the unit microscopic imaging module in

[0055] Figure 3 is Figure 1 a schematic structural diagram of a second embodiment of the unit microscopic imaging module in

[0056] Figure 4 is Figure 1 a schematic structural diagram of a third embodiment of the unit microscopic imaging module in

[0057] Figure 5 is a schematic structural diagram of a second embodiment of a microscopic imaging system of the present invention;

[0058] Figure 6 is a schematic structural diagram of a third embodiment of a microscopic imaging system of the present invention;

[0059] Figure 7 is Figure 5 or Figure 6 a schematic cross-sectional structure diagram of a first embodiment of the unit microscopic imaging module in

[0060] Figure 8 is Figure 5 or Figure 6 a schematic cross-sectional structure diagram of a second embodiment of the unit microscopic imaging module in

[0061] Figure 9 is Figure 5 or Figure 6 a schematic cross-sectional structure diagram of a third embodiment of the unit microscopic imaging module in

[0062] Figure 10 is a schematic cross-sectional structure diagram of a fourth embodiment of a microscopic imaging system of the present invention;

[0063] Figure 11 It is a schematic cross-sectional structure diagram of the fifth embodiment of a microscopic imaging system of the present invention;

[0064] Figure 12 It is a schematic structure diagram of the sixth embodiment of a microscopic imaging system of the present invention;

[0065] Figure 13 is Figure 12 a schematic cross-sectional structure diagram of an embodiment of a microscopic imaging system of

[0066] Figure 14 is Figure 12 a schematic cross-sectional structure diagram of another embodiment of a microscopic imaging system of

[0067] Figure 15 It is a schematic cross-sectional structure diagram of the seventh embodiment of a microscopic imaging system of the present invention (including the dark-field illuminator for microscopic imaging of the present invention);

[0068] Figure 16 It is a schematic cross-sectional structure diagram of the eighth embodiment of a microscopic imaging system of the present invention (including the dark-field illuminator for microscopic imaging of the present invention). Detailed implementation manners

[0069] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other. The present invention will be further described in detail below with reference to the specific implementation manners.

[0070] Such as Figure 1As shown, in an embodiment of a microscopic imaging system of the present invention, it includes: a plurality of unit microscopic imaging modules 101 arranged according to a preset rule, and a data acquisition card 102 connected to the plurality of unit microscopic imaging modules 101; wherein, each unit microscopic imaging module 101 respectively includes an adjustable lens group capable of independently adjusting focus, and a photosensitive module corresponding to the adjustable lens group; on the data acquisition card 102, there are a plurality of image processing modules respectively corresponding to the plurality of unit microscopic imaging modules 101 one by one; each image processing module independently controls the focus of its corresponding adjustable lens group and acquires the data of the corresponding photosensitive module. In this embodiment, the plurality of unit microscopic imaging modules 101 can be arranged in a preset manner, for example, arranged in an array. When arranged in an array, the distance between each unit display imaging module can be reasonably set. In order to minimize the volume of the entire system and ensure accurate imaging of the object to be measured, the distance between the plurality of unit microscopic imaging modules 101 needs to be set as small as possible, and it is usually set to the minimum physical distance in the physical structure. Each unit microscopic imaging module 101 respectively includes an adjustable lens group and a photosensitive module corresponding to the adjustable lens group, and the adjustable lens group of each unit microscopic imaging module 101 can be independently adjusted for focus. The data acquisition card 102 is provided with an image processing module, which corresponds to the unit microscopic imaging module 101 one by one, and is used to control the adjustable lens groups of the plurality of unit microscopic imaging modules 101 to independently focus, and at the same time acquire the corresponding data of the photosensitive module. It constructs a centimeter-level large field of view through the plurality of unit microscopic imaging modules 101, and realizes free adjustment of the focal planes of the areas corresponding to each unit microscopic imaging module 101, and each focal plane is in a discrete state of non-same focus, which can reduce the requirements for ensuring the flatness of the physical assembly during the assembly process of the plurality of unit microscopic imaging modules 101, reduce the pressure in the production assembly process, and improve the assembly efficiency. At the same time, through the independent adjustable focus design of the unit microscopic imaging module 101, it can be used for high-quality imaging of uneven samples, and ensure that the focal planes in the field of view are in the best state during a single imaging process.

[0071] Optionally, the microscopic imaging system of the present invention further includes a fixing mechanism 109 provided on the data acquisition card 102, and each adjustable lens group is independently fixed to the data acquisition card 102 through the fixing mechanism 109. Specifically, the fixing mechanism 109 is provided on the data acquisition card 102, and the adjustable lens group is fixed through the fixing mechanism 109. Each adjustable lens group can be independently fixed through the fixing mechanism 109 without affecting each other.

[0072] As Figures 2 to 4As shown, optionally, the adjustable lens group includes a second lens group 1012 disposed close to the photosensitive module and a first lens group 1011 disposed away from the photosensitive module; each unit microscopic imaging module 101 includes a focusing motor 1013, and the focusing motor 1013 is connected to any one of the first lens group 1011 and the second lens group 1012, and can adjust the relative positions of the first lens group 1011 and the second lens group 1012 to achieve focusing of the adjustable lens group. In this embodiment, the adjustable lens group includes a first lens group 1011 and a second lens group 1012, wherein the second lens group 1012 is disposed close to the photosensitive module, and the first lens group is disposed away from the photosensitive module. A focusing motor 1013 corresponding to the adjustable lens group is disposed in each unit microscopic imaging module 101, and the relative positions of the first lens group 1011 and the second lens group 1012 are adjusted by the focusing motor 1013 to achieve focusing of the adjustable lens group. The photosensitive module includes a circuit board 1016 fixed on a data acquisition card and a photosensitive chip 1015 disposed on the circuit board 1016. The circuit board 1016 includes, but is not limited to, a printed circuit board, a flexible circuit board, etc. The photosensitive chip 1015 is fixedly placed at the image-side focal plane of the second lens group 1012, and the light converged by the second lens group 1012 is incident on the photosensitive chip 1015, and the photosensitive chip 1015 performs photoelectric conversion on the sensed light. The photosensitive chip 1015 is a planar photoelectric device. For example, the photosensitive chip 1015 is a CMOS image sensor or a CCD image sensor. The first lens group 1011 and the second lens group 1012 form an approximately infinity-corrected microscope structure, and both the first lens group 1011 and the second lens group 1012 have positive optical powers. The first lens group 1011 is equivalent to the objective lens of the microscope, and the second lens group 1011 is equivalent to the tube lens of the microscope. The focusing motor 1013 drives one of the first lens group 1011 and the second lens group 1012 to achieve focusing, that is, to change the object plane or image plane position of the imaging module. The object plane closest to the imaging module is called the near-focus object plane, and the object plane farthest from the imaging module is called the far-focus object plane. And the direction away from this module is defined as the positive direction. There is a limiting surface on the object side of the adjustable lens group. When there is a protective glass on the object side of the adjustable lens group, the object-side surface of the protective glass is the limiting surface. When there is no protective glass on the object side of the lens group, the object-side end face of the housing of the imaging module or other mechanical structures cooperating with the imaging module is the limiting surface. The near-focus object plane is within the range of ±50 μm of the limiting surface, and the distance between the far-focus object plane and the limiting surface is ≥220 μm. The stroke of the focusing motor 1013 is ≥300 μm and ≤600 μm. In the focusing range, the minimum distance between the first lens group 1011 and the second lens group 1012 (the minimum distance between the optical lens surfaces, rather than the distance between the mechanical housings of the lens groups) is ≥50 μm, and when there is a protective glass, the minimum distance between the first lens group and the protective glass is ≥30 μm.There are four beneficial effects of adopting the above parameters: 1) The near-focus object plane can cover the close-range area of the protective glass, enabling imaging of objects in close proximity. At the same time, the far-focus object plane can exceed the commonly used cover glass of the microscope, meeting the requirements of biomedical imaging and also avoiding the offset of the limiting surface caused by the thickness tolerance of the protective glass; 2) The motor stroke can effectively cover the dimensional tolerances of various components in the module due to processing and installation, improving the manufacturability of mass production; 3) A minimum gap is reserved between the first lens group 1011 and the protective glass and the second lens group 1012, enhancing the reliability of the module and preventing internal components of the module from colliding and being damaged when the motor exceeds the rated stroke; 4) While achieving the above two beneficial effects, miniaturization of the module is realized. The focusing motor 1013 of each unit microscopic imaging module 101 can be controlled individually and a single microscopic image can be read, thus ensuring that the imaging focal planes of the entire array system are in a discrete state of non-parallel focus.

[0073] Optionally, the focusing motor 1013 and any one of the lens groups connected thereto are encapsulated into an independent encapsulation module. The focusing motor 1013 can be encapsulated together with the first lens group 1011 or the second lens group 1012 connected thereto to form an independent encapsulation module. This independently encapsulated module can be directly fixed or fixed to the data acquisition card 102 through a fixing mechanism 109. The independently encapsulated module can also be fixed to the fixing mechanism 109 in a snap-fit manner to achieve independent maintenance and replacement of the encapsulation module.

[0074] Optionally, the other lens group in the first lens group 1011 and the second lens group 1012 is fixedly connected to the fixing mechanism 109; specifically, the other lens group not connected to the focusing motor 1013 can be fixedly connected to the fixing mechanism 109. For example, the encapsulation of the lens group and the fixing mechanism 109 are integrally designed.

[0075] Optionally, the fixing mechanism 109 includes a lens group fixing seat 1014, and the other lens group in the first lens group 1011 and the second lens group 1012 is threadedly connected to the lens group fixing seat 1014. Specifically, the fixing mechanism 109 can also be provided with a lens group fixing seat 1014. Threads are provided on the lens group fixing seat 1014, and corresponding threads can be provided on the other lens group not connected to the focusing motor 1013 to achieve threaded connection with the threads of the lens group fixing seat 1014 provided by the fixing mechanism 109.

[0076] Optionally, as Figure 1 、 Figure 5 、 Figure 6 and Figure 12As shown, the microscopic imaging system of the present invention further includes an illumination module corresponding to a plurality of unit microscopic imaging modules 101. In one embodiment, the illumination module includes a first illumination light source 104 disposed above the unit microscopic imaging module 101; that is, transmissive imaging can be achieved through the first illumination light source 104 disposed above the unit microscopic imaging module 101. In another embodiment, the illumination module includes a second illumination light source uniformly disposed around each unit microscopic imaging module 101. By reasonably setting the positions of the light guiding structure 202 and the second illumination light source, reflective imaging or transmissive imaging can be achieved.

[0077] Optionally, the first illumination light source includes a fluorescence excitation light source, and the unit microscopic imaging module further includes a fluorescence excitation filter 302 disposed on the lower end face, inside or upper end face of the adjustable lens group; as Figures 7 to 9 shown, when a fluorescence excitation light source is adopted, a corresponding fluorescence excitation filter 302 also needs to be disposed in the unit microscopic imaging module 101. The setting position of the fluorescence excitation filter 302 can be disposed on the lower end face, inside or upper end face of the adjustable lens group. The fluorescence excitation light source is an obliquely incident laser 1041 or an LED lamp bead 1042 of a specific wavelength band, and is fixed in the microscopic imaging system through a mechanical structure. The light source illuminates the fluorescence detection sample in a transmissive, obliquely incident, side-incident, etc. manner to excite the fluorescence of the sample. A fluorescence excitation filter 302 for fluorescence detection is added in the unit microscopic imaging module 101. The fluorescence excited by the sample passes through the fluorescence excitation filter 302, and the photosensitive module collects the fluorescence detection image of the sample. When the light source adopts an obliquely incident laser 1041, one laser emitter can be used to provide a transmissive light source for all the unit microscopic imaging modules 101. The setting position of the obliquely incident laser 1041 can be arranged to ensure that it can cover all the unit microscopic imaging modules 101 as much as possible. In some embodiments, since the number of unit microscopic imaging modules 101 is relatively large and the area after their arrangement is large, the number of the obliquely incident lasers 1041 can be set to be multiple, and they are incident from different angles to ensure that the imaging light sources of the unit microscopic imaging modules 101 meet the requirements.

[0078] Optionally, as Figure 10 and Figure 11The first illumination light source includes a first dark-field illuminator disposed above the adjustable lens group and corresponding to the adjustable lens group; when the first dark-field illuminator is used, the first dark-field illuminator includes an array of LED light sources 4011 with a first circular through-hole in the housing or a white-light backlight source 4013 with a second circular through-hole in the housing. The first circular through-hole and the second circular through-hole are arranged opposite to the adjustable lens group; that is, the specific structure of its transmissive illumination diffused reflection illuminator can be an LED light source 4011 with a matte black housing with a round hole or a white-light backlight source 4013 with a round hole. The round holes in the housing are arranged corresponding to the unit microscopic imaging module 101. The optical axes of the adjustable lens groups of each unit microscopic imaging module 101 pass through the centers of the round holes in its housing, and the sizes of the round holes are larger than the field-of-view range 10111 of each unit microscopic imaging module 101. The LED light sources 4011 are arranged in an array on a plane inside the housing 4012 at a certain distance from the hole-bearing surface around the round holes in the housing. The range of its array arrangement distance is such that most of the emitted light 40111 does not directly enter each adjustable lens group after obliquely irradiating the sample at a large angle. The surface of the PCB substrate 40112 to which the LED light sources 4011 are circuit-connected to the module should be matte black, or circular through-holes need to be arranged within the range where the field-of-view cone angles 10111 of each adjustable lens group fall on the circuit board after passing through the round holes in the housing, exposing the matte black surface of the housing 4012. When using the backlight 4013 with round holes for illumination, the backlight board is arranged on a plane within a certain distance range from the hole-bearing surface of the housing, and is blocked by the stray light baffle 4014, so that the light emitted from the effective light-emitting plane of the backlight 4013 is directed towards the observed sample and the module lens direction. The round holes on the backlight 4013 are arranged corresponding to the module array form and are coaxial with the round holes on the housing surface. The matte black surface of the housing is exposed within the lens field of view through the round holes in the backlight, and the edges of the holes are outside the range where the lens cone angle falls on the backlight surface after passing through the round holes in the housing. When a complete backlight 4016 is added behind the backlight 4013 with round holes, its light intensity is strong, and the effective light-emitting surface of the complete backlight 4016 covers the semi-transparent black diffused reflection thin sheet material 4015 and faces the sample and the lens direction. The emitted light from a partial area of the light-emitting surface of the complete backlight 4016 exposed behind the round holes of the backlight with round holes can pass through the semi-transparent black diffused reflection thin sheet 4015 to perform transmissive illumination on the sample. Then, this dark-field illuminator simultaneously has the function of bright-field transmissive illumination, realizing the integration and miniaturization of the dark-field illuminator and the bright-field illuminator. The above illumination light source is circuit-connected to each unit microscopic imaging module 101 by means of wires and the like.

[0079] Optionally, such as Figures 13 to 16As shown, the second illumination light source is provided on the upper end surface of the fixing mechanism 109; the second illumination light source may be LED lamp beads 201 arranged in an array around the unit microscopic imaging module 101, and the light emitted by it is propagated through a light guide structure 202 made of a transparent or translucent material. A positioning surface is provided on the object side end surface of the light guide structure 202 to limit the sample, so that the surface of the sample is located on the object side focal plane of the adjustable lens group. A light guide surface with a special surface shape is provided around the adjustable lens group, so that the illumination in the object observation area is sufficient, uniform and soft. The light is reflected by the surface of the sample, and the unit microscopic imaging module 101 collects the reflected illumination image of the sample.

[0080] Optionally, the second illumination light source further includes a second dark field illuminator provided above the adjustable lens group and corresponding to the adjustable lens group. The second dark field illuminator used includes a bright and dark field substrate 4017 and a dark field black background patch 4018. The dark field black background patch 4018 is matched with the size of the adjustable lens group, and the dark field black background patch 4018 is arranged closer to or farther from the adjustable lens group relative to the bright and dark field substrate 4017. Specifically, the second dark field illuminator, that is, the reflective dark field illuminator, can be a white diffuser with a black diffusive circular surface. A plurality of black diffusive circular surfaces are arranged in an array corresponding to the unit microscopic imaging module 101. The centers of the circles of each black diffusive surface pass through the optical axes of the respective adjustable lens groups, and the size of the circles is larger than the field of view range of the unit microscopic imaging module 101. The reflector is close to the back of the observed sample. The above-mentioned reflective illumination light source is used in combination to illuminate the white diffusive surface around the black circular area. Some large-angle diffused light rays 2011 illuminate the observed sample again without directly entering the adjustable lens group, and the diffused light generated on the surface of the observed sample enters the lens to generate a dark field illumination image of the sample.

[0081] Optionally, the image processing module includes an image signal processing unit, a data buffer unit, a motor control unit, and a data transmission interface; the image signal processing unit is directly connected to the on-board circuit of the data acquisition card 102 to form an electrical connection. The data acquisition card 102 and the motor control unit are respectively connected to the photosensitive module and the focusing motor 1013 through a flexible cable 106. The microscopic imaging system further includes a main controller 105 and an image display unit 103. The main controller 105 is connected to the image processing unit through the data buffer unit, and the main controller 105 is connected to the image signal processing unit through a first bus 108 and to the image display unit through a second bus. The image signal processing unit can also be integrated into the photosensitive module. The photosensitive module is connected to the data acquisition card 102 through a flexible cable 106 and then directly connected to the main controller 105 through the interface circuit of its flexible cable 108.

[0082] Optionally, the main controller 105 and the image display unit 103 are integrated into an intelligent terminal. That is, image data can be received through the intelligent terminal for processing and display.

[0083] The following will Figures 1 to 16 describe in detail a specific embodiment of a microscopic imaging system of the present invention.

[0084] Embodiment 1

[0085] As Figure 1 shown, in this embodiment, the microscopic imaging system includes six unit microscopic imaging modules 101, and the six unit microscopic imaging modules 101 are arranged in an array structure with the minimum physical distance and fixed on the data acquisition card 102 through a fixing mechanism 109. The six unit microscopic imaging modules 101 are respectively connected to the data acquisition card 102 through an on-board cable interface 106. Above the unit microscopic imaging module 101, there is a carrier platform for carrying the sample to be measured ( Figure 1 not shown), and the sample 107 to be measured can be arranged on this carrier platform. At the same time, above the carrier platform, there is an illumination light source 104. On the data acquisition card 102, there are six independent image processing chips ( Figure 1 not shown) that respectively and independently control the photosensitive module and the focusing motor 1013 ( Figure 1 not shown) in the six unit microscopic imaging modules 101. The image processing chip obtains photosensitive data through the photosensitive module for corresponding processing. The data acquisition card 102 is provided with an interface bus 108, and the data acquisition card 102 is connected to the main controller 105 through this interface bus 108. The main controller 105 obtains the photosensitive data of the six unit microscopic imaging modules 101 through this interface bus 108 for corresponding processing, and forms an image through the image display unit 103. The image processing chip on the data acquisition card 102 includes an independent RAM and an ISP chip. The main controller 105 is composed of an MTK6797 chip and a data storage ROM, and is used to analyze and store image data and drive the image display unit 103 to display the result. The image display unit 103 uses a 5.5-inch OLED screen with a resolution of 1920*1080. In this embodiment, the illumination light source 104 can use an LCD backlight board based on 2 LED light-emitting chips with a color temperature of 5000k and a power of 0.06W to provide transmitted illumination for the sample 107.

[0086] Figures 2 to 4 For Figure 1 different embodiments of the unit microscopic imaging module 101 in

[0087] As Figure 2As shown, in this embodiment, the adjustable lens group includes a first lens group 1011 close to the photosensitive module and a second lens group 1012 far from the photosensitive module. The focal length f1 of the first lens group 1011 is 2.2 mm, and the focal length f2 of the second lens group 1012 is 3 mm. The distance TTL from the object plane to the image plane of the adjustable lens group on the optical axis is 8 mm, and the distance TD from the object side (close to the object to be measured) surface to the image side (close to the photosensitive module) surface on the optical axis is 6 mm. The first lens group 1011 is mounted on the movable carrier of the focusing motor 1013 by dispensing or other fixing means. The focusing motor 1013 can be a voice coil motor, an ultrasonic motor, a shape memory alloy motor, etc. The stroke of the focusing motor 1013 is 300 μm. There is a lens fixing seat 1014 in the unit microscopic imaging module, which has internal threads, and the second lens group 1012 has external threads, and the two are connected by threads. During actual imaging, the focusing motor 1013 drives the first lens group 1011 to move to achieve the focusing function, while the position of the second lens group 1012 in the imaging optical path is fixed. The photosensitive module is arranged on the circuit board 1016, and the circuit board includes but is not limited to a printed circuit board, a flexible circuit board, etc. The photosensitive module is fixedly placed at the image-side focal plane of the second lens group 1012, and the light converged by the second lens group 1012 is incident on the photosensitive module, and the photosensitive module performs photoelectric conversion on the sensed light. The photosensitive chip 1015 used by the photosensitive module is a planar photoelectric device, and the photosensitive chip 1015 can also be a CMOS image sensor or a CCD image sensor.

[0088] As Figure 3 shown, in this embodiment, on the basis of the above embodiment, the focusing motor 1013 is placed below, and the second lens group 1012 is mounted on the movable carrier of the focusing motor 1013 by dispensing or other fixing means. The first lens group 1012 has external threads and is threadedly connected to the lens fixing seat 1014 with internal threads. During actual imaging, the focusing motor 1013 drives the second lens group 1012 to move to achieve the focusing function, while the position of the first lens group 1011 in the imaging optical path is fixed. As Figure 4 shown, in this embodiment, on the basis of the above embodiment, the first lens group 1011 is connected to the mechanical fixing mechanism 109 through its package. The second lens group 1012 driven by the focusing motor 1013 is a separate module. Although it has a certain impact on the object-side focusing range, different combinations of the first lens group 1011 and the second lens group 1012 can be adopted according to different actual application requirements, and it is not limited to the existing microscopical imaging module with an integral package.

[0089] Embodiment 2

[0090] As Figure 5As shown, on the basis of Embodiment 1, the illumination light source provided above the bearing platform is a fluorescence excitation light source, and this fluorescence excitation light source can adopt an obliquely incident laser 1041. It can adopt a laser with a wavelength of 488 nm. When using a fluorescence excitation light source, a corresponding fluorescence excitation filter 302 is added to each unit microscopic imaging module 101.

[0091] Embodiment 3

[0092] As Figure 6 shown, on the basis of Embodiment 1, the illumination light source provided above the bearing platform is a fluorescence emission light source, and this fluorescence excitation light source can adopt an LED light-emitting chip 1042. It can adopt an LED light-emitting chip with a wavelength of 488 nm. When using a fluorescence excitation light source, a corresponding fluorescence excitation filter 302 is added to each unit microscopic imaging module 101.

[0093] Figures 7 to 9 For Figure 5 and Figure 6 the different embodiments of the unit microscopic imaging module 101 in the embodiments shown.

[0094] As Figure 7 shown, in this embodiment, the fluorescence excitation filter 302 is located between the first lens group 1011 and the sample 107 in the unit microscopic imaging module 101. As Figure 8 shown, in this embodiment, the fluorescence excitation filter 302 is located between the first lens group 1011 and the second lens group 1012 in the unit microscopic imaging module 101. As Figure 9 shown, the fluorescence excitation filter 302 is located between the second lens group 1012 and the photosensitive module in the unit microscopic imaging module 101.

[0095] Embodiment 4

[0096] As Figure 10 shown, on the basis of Embodiment 1, the illumination light source provided above the bearing platform is a dark-field illuminator. Among them, the dark-field illuminator adopts an array of LED light sources 4011 with a circular hole on the shell, and the number of LED lamp beads arranged evenly around each unit module group is ≥3. Most of the light in the small-angle region of its emission angle is blocked by the shell 4012, and part of the light in the large-angle region irradiates the sample through the circular hole of the shell and does not directly enter the lens after exiting. The diffuse reflection light generated by the edges with a height difference in some areas of the sample enters the lens module, and the background of the field of view in the module is the black matte surface of the housing backplane exposed by the circular through-hole of the LED light source printed circuit board 40112, so as to obtain a dark-field illumination image of the sample sheet.

[0097] Embodiment 5

[0098] As Figure 11As shown, on the basis of Embodiment 1, the illumination light source arranged above the bearing platform is a dark-field illuminator. The dark-field illuminator uses a perforated high-brightness white backlight source 4013. The bottom surface of the hole can be made of a material similar to a black diffuse reflection surface. A stray light baffle 4014 is added to the partial light-emitting annular surface of the hole wall to block it, so as to avoid direct illumination of the black background at the bottom of the hole. At the same time, the light-emitting area and the angle of the light irradiating the sample are controlled. After the sample is irradiated, the diffuse reflection light generated by the edges with a height difference in some areas enters the lens module, thereby obtaining a dark-field illumination image. When the bottom surface of the perforated backlight source 4013 of this dark-field illuminator is a semi-transparent thin sheet 4015 of a black diffuse reflection surface, a complete backlight source 4016 can be added behind it. Close the perforated backlight source 4013 and turn on the complete backlight source 4013, and the sample can be illuminated by transmission, that is, this dark-field illuminator also has the function of bright-field illumination.

[0099] Embodiment 6

[0100] As Figure 12 shown, on the basis of Embodiment 1, its illumination light source uses a reflection illumination light source. The reflection illumination light source uses a white LED lamp bead 201 as the light source. The overall PCB circuit board is fixed to the module array formed by each unit module, and the LED lamp beads are evenly distributed around each unit module on the PCB circuit board to ensure that the illumination conditions of the samples in the field of view of each unit module are the same. The light guide structure 202 is fixed above the reflection illumination light source. The light-transmitting rate of the body material is relatively high, and its surface is subjected to atomization treatment. A conical surface is provided around the lens group to improve the illumination effect, reduce stray light, and make the illumination more uniform. Its upper end face is the sample positioning surface. Good imaging effects can be achieved by observing samples with low light transmittance through reflection illumination.

[0101] Figures 13 to 14 For Figure 12 different embodiments of the unit microscopic imaging module 101 in

[0102] As Figure 13 shown, in this embodiment, its unit microscopic imaging module 101 uses an overall package module in which a focusing motor 1013 drives the first lens group 1011. The reflection illumination light source 201 is connected to the upper end face of the mechanical fixing structure 109, and its emitted light 2011 irradiates the sample surface (the sample surface is limited by the upper end face of the light guide structure 202) after passing through the conical hole surface of the light guide structure 202, and the sample image is obtained through the camera module. An overall package module of the unit microscopic imaging module with the focusing motor 103 placed below can also be used as an embodiment of this reflection illumination.

[0103] As Figure 14As shown, in this embodiment, the unit microscopic imaging module adopts a non-integrally packaged module. The mechanical fixing structure 109 in this embodiment is only used to fix the lower autofocus module and connect the reflective illumination light source 201. The first lens group 1011 is connected to the light guiding structure 202 through its packaging.

[0104] Embodiment 7

[0105] As Figure 15 shown, on the basis of Embodiment 6, the dark field illuminator is a reflector with a circular black background in the middle area, which is larger than the field of view of the unit microscopic imaging module, and the surrounding is a white diffuse reflection surface. In the case of dark field illumination, the surface of the entire plate with the black area side is attached to the back of the sample slide. The centers of the circular black background, the sample, and the lens are on the same axis. The light source is the LED lamp bead 201 of the reflective illumination unit module. Its emitted light 2011 passes through the light guiding structure 202, the air gap, and the sample slide, and then irradiates the white diffuse reflection surface around the circular black background. Part of the diffuse reflected light enters the slide and then irradiates the sample. After the sample is irradiated, the diffuse reflected light generated by the edges with height differences in some areas enters the lens module. However, most of the diffuse reflected light generated by the rest of the reflector in the slide undergoes total reflection due to the large exit angle on the slide surface and cannot exit the slide, but propagates to its edge inside the slide without entering the lens. Thus, the system obtains a dark field illumination image.

[0106] Embodiment 8

[0107] As Figure 16 shown, on the basis of Embodiment 6, this bright field diffuse reflection is a concave structure, and the distance from its white diffuse reflection surface to the positioning surface is 0.5 - 5 mm.

[0108] It can be understood that the above embodiments only represent the preferred implementation modes of the present invention. Their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the present invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, the above technical features can be freely combined, and several deformations and improvements can also be made, which all belong to the protection scope of the present invention. Therefore, all equivalent transformations and modifications made to the scope of the claims of the present invention shall fall within the scope covered by the claims of the present invention.

Claims

1. A microscopic imaging system, characterized in that, Including: A plurality of unit microscopic imaging modules arranged according to preset rules, and a data acquisition card connected to the plurality of unit microscopic imaging modules; Wherein, each of the unit microscopic imaging modules respectively includes an adjustable lens group capable of independently adjusting focus, and a photosensitive module corresponding to the adjustable lens group; A plurality of image processing modules respectively corresponding to the plurality of unit microscopic imaging modules are provided on the data acquisition card; each of the image processing modules independently controls the focusing of the corresponding adjustable lens group and acquires data of the corresponding photosensitive module; And an illumination module correspondingly arranged with the plurality of unit microscopic imaging modules, the illumination module including: A second illumination light source uniformly arranged around each of the unit microscopic imaging modules, and a light guide structure fixed above the second illumination light source; The second illumination light source further includes a second dark field illuminator arranged above the adjustable lens group and corresponding to the adjustable lens group; the surface of the second dark field illuminator is attached to the back of the sample slide, and the sample slide is located between the dark field illuminator and the adjustable lens group; The second dark field illuminator includes a bright and dark field substrate and a dark field black background patch; the dark field black background patch is matched with the size of the adjustable lens group, and the dark field black background patch is arranged closer to or farther from the adjustable lens group relative to the bright and dark field substrate; the periphery of the dark field black background patch has a white diffuse reflection surface; The bright and dark field substrate of the dark field illuminator further has a concave structure, and the concave structure has a white diffuse reflection surface, so that the dark field illuminator also has a bright field illumination function.

2. The microscopic imaging system according to claim 1, wherein The microscopic imaging system further includes a fixing mechanism arranged on the data acquisition card, and each of the adjustable lens groups is independently fixed to the data acquisition card through the fixing mechanism.

3. The microscopic imaging system according to claim 2, wherein The adjustable lens group includes a second lens group arranged close to the photosensitive module and a first lens group arranged far from the photosensitive module; Each of the unit microscopic imaging modules includes a focusing motor, and the focusing motor is connected to any one of the first lens group and the second lens group and can adjust the relative position of the first lens group and the second lens group to achieve the focusing of the adjustable lens group.

4. The microscopic imaging system according to claim 3, wherein The focusing motor and the any one lens group connected thereto are packaged together as an integrated packaging module.

5. The microscopic imaging system according to claim 3, characterized in that The other lens group of the first lens group and the second lens group is fixedly connected to the fixing mechanism; or The fixing mechanism includes a lens group fixing seat, and the other lens group of the first lens group and the second lens group is threadedly connected to the lens group fixing seat.

6. The microscopic imaging system according to claim 3, wherein The image processing module includes an image signal processing unit, a data buffer unit, a motor control unit and a data transmission interface; The image signal processing unit and the motor control unit are respectively correspondingly connected to the photosensitive module and the focusing motor through a flexible cable; The microscopic imaging system further includes a main controller and an image display unit. The main controller is connected to the image processing unit via the data buffer unit, and the main controller is connected to the image signal processing unit through a first bus and to the image display unit through a second bus.

7. A dark-field illuminator for microscopic imaging, characterized in that: The dark-field illuminator is disposed above and corresponding to the adjustable lens group of the unit microscopic imaging module. The surface of the dark-field illuminator is attached to the back of the sample slide, and the sample slide is located between the dark-field illuminator and the adjustable lens group. The dark-field illuminator includes a bright-dark field substrate and a dark-field black background patch. The dark-field black background patch is matched in size with the adjustable lens group, and the dark-field black background patch is disposed closer to or farther from the adjustable lens group relative to the bright-dark field substrate; the periphery of the dark-field black background patch has a white diffused reflection surface. The bright-dark field substrate of the dark-field illuminator further has a concave structure with a white diffused reflection surface inside, so that the dark-field illuminator also has a bright-field illumination function.

8. The dark-field illuminator for microscopic imaging according to claim 7, wherein: The dark-field black background patch is circular.

9. The dark-field illuminator for microscopic imaging according to claim 8, wherein: The size of the dark-field black background patch is larger than the field of view of the unit microscopic imaging module.

10. The dark-field illuminator for microscopic imaging according to claim 8, wherein: The center of the dark-field black background patch, the center of the sample, and the optical axis of the unit microscopic imaging module are located on the same axis.

11. The dark-field illuminator for microscopic imaging according to claim 7, wherein: In the case of bright-field illumination, the surface of the bright-dark field substrate on the side of the concave structure is attached to the back of the sample slide, and the opening of the concave structure faces the unit microscopic imaging module.

12. The dark-field illuminator for microscopic imaging according to claim 7, wherein: The dark-field black background patch and the concave structure are respectively disposed on the front and back sides of the bright-dark field substrate.

Images