A cell culture device with microcurrent stimulation function

Abstract

A cell culture device with micro-current stimulation function comprises a box, an upper rail, an upper magnetic block, an upper slider, a lower rail, a lower magnetic block, a lower slider and a stage; the box is a medical-grade polymer box or a stainless steel box; the box comprises an incubator and a control box; the incubator and the control box are arranged side by side; the upper rail, the upper magnetic block, the upper slider, the lower rail, the lower magnetic block, the lower slider and the stage are all arranged in the incubator; the stage is located in the middle of the upper rail and the lower rail; the stage is arranged horizontally; the upper slider is slidably arranged on the upper rail, and the upper magnetic block is arranged on the lower surface of the upper slider. The present invention achieves the beneficial effect of promoting nerve repair and regeneration by performing electrical stimulation in the process of culturing a variety of cells, such as tendons, ligaments, nerves, blood vessels, skin, muscles and digestive tract cells, etc., especially being able to promote the proliferation, differentiation and growth of nerve cells.

Description

Technical Field

[0001] The invention relates to the field of cell culture devices, and in particular to a cell culture device with a micro-current stimulation function. Background Art

[0002] Peripheral nerve damage is a common clinical disease, and its repair and regeneration have always been a difficult problem that has troubled neurosurgeons and material experts. When damaged over a long distance and for a long time, the damaged nerve cannot be repaired by direct suture; autologous transplantation and allogeneic transplantation have the disadvantages of secondary injury, limited sources, cross-infection and allogeneic rejection. Therefore, researchers have begun to study artificial nerve grafts. In practical applications, artificial nerve grafts are usually co-cultured with nerve cells and then transplanted to the site of nerve defects. The latest research shows that when nerve cells are stimulated by microcurrent during the culture process, they are more conducive to proliferation, differentiation and growth, thereby better promoting the repair and regeneration of damaged axonal nerves.

[0003] The use of artificial electrical stimulation devices to culture nerve cells in vitro can be more conducive to the tissue engineering of nerve conduits. At present, the conventional method for cell electrical stimulation is to use a primary cell to generate an electromotive force through a chemical reaction between an electrode and an electrolyte, thereby generating a weak current. For example, a Chinese patent (publication number: CN201920576816.7) provides a neural electrical stimulation electrode assembly and a neural electrical stimulation device, which generates a weak current by utilizing the chemical reaction between the electrode and the electrolyte in the primary cell. This method of generating current through chemical reactions has defects. Since the chemical reaction rate changes with the reaction process, the generated current may change, and the electrolyte and electrodes need to be replaced within a period of time. In addition, side reactions may occur in the primary cell, resulting in reduced battery charge and discharge efficiency, capacity life loss, and performance degradation. For example, a Chinese patent (publication number: CN92242285.0) provides a copper-zinc primary battery demonstrator composed of a conjoined double cylinder, a salt bridge, a copper sheet, a zinc sheet, a special small light bulb, and positive and negative electrode terminals. This method uses zinc as the negative electrode of the primary cell. When pure zinc is used as an electrode, a small amount of bubbles are generated on the zinc sheet; when impure zinc is used as an electrode, more bubbles are generated on the zinc sheet. This is because the zinc on the zinc sheet forms many tiny primary cells with surface impurities, resulting in side reactions. This cannot provide a stable and continuous power supply for the electrical stimulation device, which is not conducive to the normal culture of cells.

[0004] Therefore, in order to solve the above defects, it is necessary to provide a cell culture device with a continuous, stable, safe and environmentally friendly electrical stimulation function. Summary of the invention

[0005] (I) Purpose of the invention

[0006] In order to solve the technical problems existing in the background technology, the present invention proposes a cell culture device with microcurrent stimulation function, which can better promote various cells, such as tendons, ligaments, nerves, blood vessels, skin, muscles and digestive tract cells, through electrical stimulation, and is particularly beneficial to the proliferation, differentiation and growth of nerve cells, thereby promoting nerve repair and regeneration to achieve a more ideal effect.

[0007] (II) Technical solution

[0008] To solve the above problems, the present invention proposes a cell culture device with micro-current stimulation function, comprising a box, an upper guide rail, an upper magnetic block, an upper slider, a lower guide rail, a lower magnetic block, a lower slider and a stage;

[0009] The box body is a medical grade polymer box body or a stainless steel box body; the box body includes an incubator and a control box; the incubator and the control box are arranged side by side;

[0010] The upper guide rail, the upper magnetic block, the upper slider, the lower guide rail, the lower magnetic block, the lower slider and the loading platform are all arranged in the incubator; the loading platform is located in the middle of the upper guide rail and the lower guide rail; the loading platform is arranged horizontally;

[0011] The upper slider is slidably arranged on the upper guide rail, and the upper magnetic block is arranged on the lower surface of the upper slider; the lower slider is slidably arranged on the lower guide rail, and the lower magnetic block is arranged on the upper surface of the lower slider; the upper magnetic block and the lower magnetic block have opposite magnetic poles facing the stage;

[0012] A control device for controlling the sliding of the upper slider and the lower magnetic block is arranged inside the control box.

[0013] Preferably, the distance between the loading platform and the upper guide rail and the lower guide rail is 1-50 cm; the distance between the bottom surface of the upper magnetic block and the loading platform is X; the distance between the upper surface of the lower magnetic block and the loading platform is X; X is 0.5-50 cm.

[0014] Preferably, the sliding speed of the upper slider and the lower slider is 1-100 cm / s.

[0015] Preferably, the two ends of the upper guide rail and the lower guide rail are respectively M end and N end;

[0016] In the initial state: the upper slider is slidably arranged at the M end position of the upper guide rail, and the lower slider is slidably arranged at the M end position of the lower guide rail;

[0017] In the motion state, the upper slider moves synchronously with the lower slider, along a reciprocating motion from M to N and then from N to M; the moving direction and speed of the upper slider and the lower slider are always the same;

[0018] A conductive device with a length of L and a culture dish inoculated with cells are placed at the center of the stage;

[0019] The moving speed of the upper slider and the lower slider is V; the conductive device cuts the magnetic flux lines, and the calculation formula of the current I1 is:

[0020] I1=B×L×V×Sinα / R;

[0021] α is the angle between the conductive device and the vertical magnetic flux lines;

[0022] R is the resistance of the conductive device;

[0023] B is the magnetic induction intensity between the upper magnetic block and the lower magnetic block;

[0024] The conductive device is a metal wire, a metal tube or a metal rod.

[0025] Preferably, the two ends of the upper guide rail and the lower guide rail are respectively M end and N end;

[0026] In the initial state: the upper slider is slidably set at the M end position of the upper guide rail, and the lower slider is slidably set at the N end position of the lower guide rail;

[0027] In the motion state, the upper slider reciprocates from M to N and then from N to M; the lower slider reciprocates from N to M and then from M to N.

[0028] The upper slider and the lower slider always move in opposite directions and at the same speed;

[0029] A conductive device with a length of L and a culture dish inoculated with cells are placed at the center of the stage;

[0030] The moving speed of the upper and lower sliders is V; the conductive device cuts the magnetic flux lines, and the calculation formula of the current I2 is:

[0031] I2=B×L×V×Sinα / R;

[0032] α is the angle between the conductive device and the vertical magnetic flux lines;

[0033] R is the resistance of the conductive device;

[0034] B is the magnetic induction intensity between the upper magnetic block and the lower magnetic block;

[0035] The conductive device is a metal wire, a metal tube or a metal rod.

[0036] Preferably, the two ends of the upper guide rail and the lower guide rail are respectively M end and N end;

[0037] In the initial state: the lower slider is fixedly set at the M end position or the N end position of the lower guide rail;

[0038] In the moving state, the upper slider reciprocates from M to N and then from N to M; the lower slider remains stationary;

[0039] The conductive device with a length of L and the culture dish with cells are placed at the center of the stage; the moving speed of the upper slider is V; the conductive device cuts the magnetic flux lines, and the calculation formula of the current I3 is:

[0040] I3=B×L×V×Sinα / R;

[0041] α is the angle between the conductive device and the vertical magnetic flux lines;

[0042] R is the resistance of the conductive device;

[0043] B is the magnetic induction intensity between the upper magnetic block and the lower magnetic block;

[0044] The conductive device is a metal wire, a metal tube or a metal rod.

[0045] The present invention can better promote the proliferation, differentiation and growth of various cells, such as tendons, ligaments, nerves, blood vessels, skin, muscles and digestive tract cells, especially nerve cells, through electrical stimulation, thereby promoting nerve repair and regeneration to achieve a more ideal effect;

[0046] The present invention has a simple device structure, low production and maintenance costs, and is easy to promote industrialization;

[0047] The present invention sets the movement direction, trajectory and speed of the upper and lower magnetic blocks through a program to change the magnetic field and realize automatic control of the cell culture process;

[0048] The present invention, based on the principle of "magnetoelectricity", can maintain stable changes in the magnetic field and generate stable current without the need for primary batteries, electrolytes and electrodes, and is sustainable, stable and environmentally friendly. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 This is a schematic diagram of the structure of the cell culture device with microcurrent stimulation function proposed by the present invention.

[0050] Figure 2 This is a schematic diagram of the first embodiment of the cell culture device with microcurrent stimulation function proposed by the present invention.

[0051] Figure 3 This is a schematic diagram of a second embodiment of the cell culture device with microcurrent stimulation function proposed by the present invention.

[0052] Figure 4 This is a schematic diagram of a third embodiment of the cell culture device with microcurrent stimulation function proposed by the present invention.

[0053] Figure 5The cell culture device with microcurrent stimulation function proposed by the present invention shows that the nerve cells grow well after being cultured in the device for three days, indicating that the device proposed by the present invention is beneficial to the growth and proliferation of nerve cells.

[0054] Figure 6 The growth state of nerve cells after three days of culture in a normal environment is compared to that of cells cultured in the device of the present invention for the same period of time, indicating that the device proposed by the present invention is conducive to the cultivation of nerve cells.

[0055] Figure 7 In the cell culture device with microcurrent stimulation function proposed by the present invention, the instantaneous electromotive force generated by 10 magnesium wires with a length of 10 cm during the process of passing through the device changes with time. When the magnets of the upper and lower guide rails move in a straight line at a uniform speed in the same direction, the instantaneous electromotive force generated when the magnesium wire cuts the magnetic flux lines is about 0.35V, which lasts for about 4 seconds. At the moment of changing direction, the electromotive force becomes 0.1V. It can be seen from the figure that the change is relatively regular and is applicable to the theoretical calculation formula proposed by the present invention. It proves that the device proposed by the present invention can generate microcurrents that are beneficial to the cultivation of nerve cells. DETAILED DESCRIPTION

[0056] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with specific embodiments and with reference to the accompanying drawings. It should be understood that these descriptions are only exemplary and are not intended to limit the scope of the present invention. In addition, in the following description, the description of well-known structures and technologies is omitted to avoid unnecessary confusion of the concept of the present invention.

[0057] like Figure 1-7 As shown, a cell culture device with microcurrent stimulation function proposed by the present invention includes a box 4, an upper guide rail 11, an upper magnetic block 21, an upper slider 31, a lower guide rail 12, a lower magnetic block 22, a lower slider 32 and a stage 6;

[0058] The box body 4 is a medical grade polymer box body or a stainless steel box body; the box body 4 includes an incubator 41 and a control box 42; the incubator 41 and the control box 42 are arranged side by side;

[0059] The upper guide rail 11, the upper magnetic block 21, the upper slider 31, the lower guide rail 12, the lower magnetic block 22, the lower slider 32 and the stage 6 are all arranged in the incubator 41; the stage 6 is located in the middle of the upper guide rail 11 and the lower guide rail 12; the stage 6 is arranged horizontally;

[0060] The upper slider 31 is slidably disposed on the upper guide rail 11, and the upper magnetic block 21 is disposed on the lower surface of the upper slider 31; the lower slider 32 is slidably disposed on the lower guide rail 12, and the lower magnetic block 22 is disposed on the upper surface of the lower slider 32; the upper magnetic block 21 and the lower magnetic block 22 have opposite magnetic poles facing the stage 6;

[0061] A control device for controlling the sliding of the upper slider 31 and the lower magnetic block 22 is disposed inside the control box 42 .

[0062] In an optional embodiment, the distance between the loading platform 6 and the upper guide rail 11 and the lower guide rail 12 is 1-50 cm;

[0063] The distance between the bottom surface of the upper magnetic block 21 and the stage 6 is X; the distance between the upper surface of the lower magnetic block 22 and the stage 6 is X; X is 0.5-50 cm.

[0064] In an optional embodiment, the sliding speed of the upper slider 31 and the lower slider 32 is 1-100 cm / s.

[0065] In the present invention, according to the principle of "magnetoelectricity", when a part of the conductor of a closed circuit moves to cut the magnetic flux lines, an induced current will be generated on the conductor. In a magnetic field environment, as long as a conductive material (such as medical human tubes, metal wires, etc.) is placed around the cells and the magnetic field is changed, the purpose of automatically cutting the magnetic flux lines can be achieved, thereby generating an induced current, so that the cells receive electrical stimulation, thereby better promoting their proliferation, differentiation and growth. Compared with the original battery method, the device based on the principle of "magnetoelectricity" does not have a chemical reaction, so there is no change in the generated current due to the change in the chemical reaction rate, and it avoids the trouble of replacing the electrolyte and electrodes.

[0066] In the present invention, a stable current can be generated by uniformly cutting the magnetic field through the reciprocating motion of a cell culture dish or a magnetic device, which is particularly suitable for a device for promoting the regeneration of damaged nerves based on the principle of "magnetoelectricity"; a culture dish seeded with nerve cells and a conductive device are placed between the upper magnetic block 21 and the lower magnetic block 22, and the magnetic field is changed by the movement of the upper magnetic block 21 and the lower magnetic block 22, and the conductive device automatically cuts the magnetic lines of force; further, by changing the magnetic field, an induced current is generated around the conductive device, so that the nerve cells receive electrical stimulation and are better cultured, thereby achieving the purpose of promoting the repair and regeneration of damaged nerves.

[0067] The present invention achieves the beneficial effect of promoting nerve repair and regeneration by performing electrical stimulation during the culture process of various cells, such as tendons, ligaments, nerves, blood vessels, skin, muscles and digestive tract cells, etc., especially promoting the proliferation, differentiation and growth of nerve cells.

[0068] Example 1

[0069] In the present invention, the two ends of the upper guide rail 11 and the lower guide rail 12 are respectively M end and N end;

[0070] In the initial state: the upper slider 31 is slidably disposed at the M end position of the upper guide rail 11, and the lower slider 32 is slidably disposed at the M end position of the lower guide rail 12;

[0071] In the moving state, the upper slider 31 and the lower slider 32 move synchronously, along a reciprocating motion from M to N and then from N to M; the moving direction and moving speed of the upper slider 31 and the lower slider 32 are always the same;

[0072] The conductive device with a length of L and the culture dish inoculated with cells are placed at the center of the stage 6;

[0073] The moving speed of the upper slider 31 and the lower slider 32 is V; the conductive device cuts the magnetic flux lines, and the calculation formula of the current I1 is:

[0074] I1=B×L×V×Sinα / R;

[0075] α is the angle between the conductive device and the vertical magnetic flux lines;

[0076] R is the resistance of the conductive device;

[0077] B is the magnetic induction intensity between the upper magnetic block 21 and the lower magnetic block 22;

[0078] The conductive device is a metal wire, a metal tube or a metal rod.

[0079] It should be noted that the role of electric current is to stimulate the growth of nerve cells around the catheter, thereby accelerating the regeneration and repair of damaged tissue.

[0080] Example 2

[0081] In the present invention, the two ends of the upper guide rail 11 and the lower guide rail 12 are respectively M end and N end;

[0082] In the initial state: the upper slider 31 is slidably disposed at the M end position of the upper guide rail 11, and the lower slider 32 is slidably disposed at the N end position of the lower guide rail 12;

[0083] In the moving state, the upper slider 31 reciprocates from M to N and then from N to M; the lower slider 32 reciprocates from N to M and then from M to N;

[0084] The upper slider 31 and the lower slider 32 always move in opposite directions and at the same speed;

[0085] The conductive device with a length of L and the culture dish inoculated with cells are placed at the center of the stage 6;

[0086] The moving speed of the upper slider 31 and the lower slider 32 is V; the conductive device cuts the magnetic flux lines, and the calculation formula of the current I2 is:

[0087] I2=B×L×V×Sinα / R;

[0088] α is the angle between the conductive device and the vertical magnetic flux lines;

[0089] R is the resistance of the conductive device;

[0090] B is the magnetic induction intensity between the upper magnetic block 21 and the lower magnetic block 22;

[0091] The conductive device is a metal wire, a metal tube or a metal rod.

[0092] It should be noted that the action of electric current can stimulate the damaged axonal nerve cells around the catheter, promote the proliferation, differentiation and growth of the damaged axonal nerve cells, and accelerate the regeneration and repair of the damaged axonal nerves.

[0093] Example 3

[0094] In the present invention, the two ends of the upper guide rail 11 and the lower guide rail 12 are respectively M end and N end;

[0095] In the initial state: the lower slider 32 is fixedly arranged at the M end position or the N end position of the lower guide rail 12;

[0096] In the moving state, the upper slider 31 reciprocates from M to N and then from N to M; the lower slider 32 remains stationary;

[0097] The conductive device with a length of L and the culture dish with cells are placed at the center of the stage 6; the moving speed of the upper slider 31 is V; the conductive device cuts the magnetic flux lines, and the calculation formula of the current I3 is:

[0098] I3=B×L×V×Sinα / R;

[0099] α is the angle between the conductive device and the vertical magnetic flux lines;

[0100] R is the resistance of the conductive device;

[0101] B is the magnetic induction intensity between the upper magnetic block 21 and the lower magnetic block 22;

[0102] The conductive device is a metal wire, a metal tube or a metal rod.

[0103] It should be noted that the effect of electric current can stimulate the growth of nerve cells around the catheter to a certain extent, thereby accelerating the regeneration and repair of peripheral nerves damaged over long distances and for a long time. It can also culture nerve cells in vitro and promote their proliferation, differentiation and growth.

[0104] In summary, the present invention has the following advantages:

[0105] The present invention can better promote the proliferation, differentiation and growth of various cells, such as tendons, ligaments, nerves, blood vessels, skin, muscles and digestive tract cells, especially nerve cells, through electrical stimulation, thereby promoting nerve repair and regeneration to achieve a more ideal effect;

[0106] The present invention has a simple device structure, low production and maintenance costs, and is easy to promote industrialization;

[0107] The present invention sets the motion trajectories of the upper magnetic block 21 and the lower magnetic block 22 through a program to change the magnetic field and realizes automatic control of the cell culture process;

[0108] The present invention, based on the principle of "magnetoelectricity", can maintain stable changes in the magnetic field and generate stable current without the need to use primary batteries, electrolytes and electrodes, and is green and environmentally friendly.

[0109] It should be understood that the above specific embodiments of the present invention are only used to illustrate or explain the principles of the present invention, and do not constitute a limitation of the present invention. Therefore, any modifications, equivalent substitutions, improvements, etc. made without departing from the spirit and scope of the present invention should be included in the protection scope of the present invention. In addition, the appended claims of the present invention are intended to cover all changes and modifications that fall within the scope and boundaries of the appended claims, or the equivalent forms of such scope and boundaries.

Claims

1. A cell culture device with microcurrent stimulation function, characterized in that: It comprises a box body (4), an upper guide rail (11), an upper magnetic block (21), an upper slider (31), a lower guide rail (12), a lower magnetic block (22), a lower slider (32) and a loading platform (6); The box (4) is a medical-grade polymer box or a stainless steel box; the box (4) comprises a culture box (41) and a control box (42); the culture box (41) and the control box (42) are arranged side by side; The upper guide rail (11), the upper magnetic block (21), the upper slider (31), the lower guide rail (12), the lower magnetic block (22), the lower slider (32) and the loading platform (6) are all arranged in the incubator (41); the loading platform (6) is located at the middle position between the upper guide rail (11) and the lower guide rail (12); the loading platform (6) is arranged horizontally; The upper slider (31) is slidably arranged on the upper guide rail (11), and the upper magnetic block (21) is arranged on the lower surface of the upper slider (31); the lower slider (32) is slidably arranged on the lower guide rail (12), and the lower magnetic block (22) is arranged on the upper surface of the lower slider (32); the upper magnetic block (21) and the lower magnetic block (22) face one side of the stage (6), and the magnetic poles of the upper magnetic block (21) and the lower magnetic block (22) are opposite; A control device for controlling the sliding of the upper slider (31) and the lower magnetic block (22) is provided inside the control box (42); The distance between the loading platform (6) and the upper guide rail (11) and the lower guide rail (12) is 1-50 cm; The distance between the bottom surface of the upper magnetic block (21) and the loading platform (6) is X; the distance between the upper surface of the lower magnetic block (22) and the loading platform (6) is X; X is 0.5-50 cm; The sliding speed of the upper slider (31) and the lower slider (32) is 1-100 cm / s; A conductive device with a length of L and a culture dish seeded with cells are placed at the center of the stage (6); The conductive device is a metal wire, a metal tube or a metal rod.

2. The cell culture device with microcurrent stimulation function according to claim 1, characterized in that: Assume that the two ends of the upper guide rail (11) and the lower guide rail (12) are respectively the M end and the N end; In the initial state: the upper slider (31) is slidably disposed at the M end position of the upper guide rail (11), and the lower slider (32) is slidably disposed at the M end position of the lower guide rail (12); In the moving state, the upper slider (31) and the lower slider (32) move synchronously, along a reciprocating motion from M to N and then from N to M; the moving direction and moving speed of the upper slider (31) and the lower slider (32) are always the same; The moving speed of the upper slider (31) and the lower slider (32) is V; The conductive device cuts the magnetic flux lines, and the calculation formula of the current I1 is: I1=(B×L×V×Sinα) / R; α is the angle between the conductive device and the vertical magnetic flux lines; R is the resistance of the conductive device; B is the magnetic induction intensity between the upper magnetic block (21) and the lower magnetic block (22).

3. The cell culture device with microcurrent stimulation function according to claim 1, characterized in that: Assume that the two ends of the upper guide rail (11) and the lower guide rail (12) are respectively the M end and the N end; In the initial state: the upper slider (31) is slidably disposed at the M end position of the upper guide rail (11), and the lower slider (32) is slidably disposed at the N end position of the lower guide rail (12); In the moving state, the upper slider (31) reciprocates from M to N and then from N to M; the lower slider (32) reciprocates from N to M and then from M to N. The upper slider (31) and the lower slider (32) always move in opposite directions and at the same speed; The moving speed of the upper slider (31) and the lower slider (32) is V; The conductive device cuts the magnetic flux lines, and the calculation formula of the current I2 is: I2=(B×L×V×Sinα) / R; α is the angle between the conductive device and the vertical magnetic flux lines; R is the resistance of the conductive device; B is the magnetic induction intensity between the upper magnetic block (21) and the lower magnetic block (22).

4. The cell culture device with microcurrent stimulation function according to claim 1, characterized in that: Assume that the two ends of the upper guide rail (11) and the lower guide rail (12) are respectively the M end and the N end; In the initial state: the lower slider (32) is fixedly arranged at the M end position or the N end position of the lower guide rail (12); In the moving state, the upper slider (31) reciprocates from M to N and then from N to M; the lower slider (32) remains stationary. The moving speed of the upper slider (31) is V; The conductive device cuts the magnetic flux lines, and the calculation formula of the current I3 is: I3=(B×L×V×Sinα) / R; α is the angle between the conductive device and the vertical magnetic flux lines; R is the resistance of the conductive device; B is the magnetic induction intensity between the upper magnetic block (21) and the lower magnetic block (22).

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